{"title":"Reconstruction of the thyroid follicle with long-term cultured cells.","authors":"G Fayet, S Hovsépian","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>The reconstruction of the thyroid follicle was studied in vitro using the porcine thyroid cell line ATHOS. When performed monolayers of ATHOS cells grown after more than 50 passages are cultured in a sandwich of collagen in the presence of thyrotropin (10 mU/ml), figures resembling follicles are observed under light microscopy. By electron microscopy cells appear correctly polarized, microvilli facing follicle lumina. These histiotypical figures that mimic the thyroid architecture are maintained 4-5 weeks.</p>","PeriodicalId":77445,"journal":{"name":"Thyroidology","volume":"3 3","pages":"123-5"},"PeriodicalIF":0.0000,"publicationDate":"1991-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Thyroidology","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
The reconstruction of the thyroid follicle was studied in vitro using the porcine thyroid cell line ATHOS. When performed monolayers of ATHOS cells grown after more than 50 passages are cultured in a sandwich of collagen in the presence of thyrotropin (10 mU/ml), figures resembling follicles are observed under light microscopy. By electron microscopy cells appear correctly polarized, microvilli facing follicle lumina. These histiotypical figures that mimic the thyroid architecture are maintained 4-5 weeks.
分享
京公网安备 11010802042870号
京ICP备2023020795号-1
求助内容：
应助结果提醒方式：
扫码关注我们
