Co-metabolic degradation and metabolite detection of hexabromocyclododecane by Shewanella oneidensis MR-1

IF 3.9 3区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Applied Microbiology and Biotechnology Pub Date : 2024-12-01 DOI:10.1007/s00253-023-12905-6
Syed Bilal Shah, Yiting Wang, Naveed Anwar, Syed Zaghum Abbas, Khalid Ali Khan, Song-Mei Wang, Muhammad Wajid Ullah
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Abstract

Hexabromocyclododecane (HBCD) is a widely used brominated flame retardant; however, it is a persistent organic pollutant as well as affects the human thyroid hormones and causes cancer. However, the degradation of HBCD has received little attention from researchers. Due to its bioaccumulative and hazardous properties, an appropriate strategy for its remediation is required. In this study, we investigated the biodegradation of HBCD using Shewanella oneidensis MR-1 under optimized conditions. The Box-Behnken design (BBD) was implemented for the optimization of the physical degradation parameters of HBCD. S. oneidensis MR-1 showed the best degradation performance at a temperature of 30 °C, pH 7, and agitation speed of 115 rpm, with an HBCD concentration of 1125 μg/L in mineral salt medium (MSM). The strain tolerated up to 2000 μg/L HBCD. Gas chromatography-mass spectrometry analysis identified three intermediates, including 2-bromo dodecane, 2,7,10-trimethyldodecane, and 4-methyl-1-decene. The results provide an insightful understanding of the biodegradation of HBCD by S. oneidensis MR-1 under optimized conditions and could pave the way for further eco-friendly applications.

Key points

HBCD biodegradation by Shewanella oneidensis

Optimization of HBCD biodegradation by the Box-Behnken analysis

Identification of useful metabolites from HBCD degradation

Abstract Image

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Shewanella oneidensis MR-1 对六溴环十二烷的共代谢降解和代谢物检测
摘要 六溴环十二烷(HBCD)是一种广泛使用的溴化阻燃剂,但它是一种持久性有机污染物,还会影响人体甲状腺激素并致癌。然而,六溴环十二烷的降解却很少受到研究人员的关注。由于六溴环十二烷具有生物累积性和危害性,因此需要采取适当的策略对其进行补救。在本研究中,我们使用 Shewanella oneidensis MR-1 在优化条件下研究了六溴环十二烷的生物降解。采用方框-贝肯设计(BBD)对六溴环十二烷的物理降解参数进行了优化。在矿物盐培养基(MSM)中,温度为 30 °C、pH 值为 7、搅拌速度为 115 rpm、六溴环十二烷浓度为 1125 μg/L 的条件下,S. oneidensis MR-1 的降解性能最佳。该菌株可耐受高达 2000 μg/L 的六溴环十二烷。气相色谱-质谱分析确定了三种中间产物,包括 2-溴十二烷、2,7,10-三甲基十二烷和 4-甲基-1-癸烯。这些结果使人们对 S. oneidensis MR-1 在优化条件下生物降解六溴环十二烷有了深入的了解,并为进一步的生态友好型应用铺平了道路。 要点 - 一龄单胞菌对六溴环十二烷的生物降解 - 通过盒-贝肯分析法优化六溴环十二烷的生物降解 - 从六溴环十二烷降解中鉴定有用的代谢物
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来源期刊
Applied Microbiology and Biotechnology
Applied Microbiology and Biotechnology 工程技术-生物工程与应用微生物
CiteScore
10.00
自引率
4.00%
发文量
535
审稿时长
2 months
期刊介绍: Applied Microbiology and Biotechnology focusses on prokaryotic or eukaryotic cells, relevant enzymes and proteins; applied genetics and molecular biotechnology; genomics and proteomics; applied microbial and cell physiology; environmental biotechnology; process and products and more. The journal welcomes full-length papers and mini-reviews of new and emerging products, processes and technologies.
期刊最新文献
Development of fluorescence-linked immunosorbent assay for rapid detection of Staphylococcus aureus. Novel reaction systems for catalytic synthesis of structured phospholipids. Motility of Acinetobacter baumannii: regulatory systems and controlling strategies Co-metabolic degradation and metabolite detection of hexabromocyclododecane by Shewanella oneidensis MR-1 Chitosan-based matrix as a carrier for bacteriophages.
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