Thermus thermophilus Argonaute (TtAgo) Cleavage-Aided Isothermal Amplification Strategy (TAC-IAS) for precise detection of locus-specific DNA methylation

IF 4.3 2区 化学 Q2 CHEMISTRY, MULTIDISCIPLINARY Chemical Communications Pub Date : 2025-05-16 DOI:10.1039/d5cc02012c
Fangfang Sun, Bingjie Han, Yi Zhao, Xing Chen, Hui Tian, Chenghui Liu, Yuanyuan Sun
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引用次数: 0

Abstract

A versatile TtAgo cleavage-aided isothermal amplification strategy (TAC-IAS) is devised for DNA methylation precise detection with high flexibility and universality compared to methylation-restriction endonuclease, reliably identifying methylation with an abundance of 0.1% in a large pool of unmethylated fragments.
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热嗜热菌Argonaute (TtAgo)切割辅助等温扩增策略(TAC-IAS)精确检测位点特异性DNA甲基化
与甲基化限制性内切酶相比,设计了一种多功能TtAgo切割辅助等温扩增策略(TAC-IAS),用于DNA甲基化精确检测,具有高灵活性和通用性,在大量未甲基化片段中可靠地鉴定甲基化,富集度为0.1%。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Chemical Communications
Chemical Communications 化学-化学综合
CiteScore
8.60
自引率
4.10%
发文量
2705
审稿时长
1.4 months
期刊介绍: ChemComm (Chemical Communications) is renowned as the fastest publisher of articles providing information on new avenues of research, drawn from all the world''s major areas of chemical research.
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