Harnessing peptide-cellulose interactions to tailor the performance of self-assembled, injectable hydrogels.

IF 3.2 3区 工程技术 Q2 CHEMISTRY, PHYSICAL Molecular Systems Design & Engineering Pub Date : 2025-06-02 DOI:10.1039/d5me00009b
Jessica A Thomas, Alex H Balzer, Subhash Kalidindi, LaShanda T J Korley
{"title":"Harnessing peptide-cellulose interactions to tailor the performance of self-assembled, injectable hydrogels.","authors":"Jessica A Thomas, Alex H Balzer, Subhash Kalidindi, LaShanda T J Korley","doi":"10.1039/d5me00009b","DOIUrl":null,"url":null,"abstract":"<p><p>Taking inspiration from natural systems, such as spider silk and mollusk nacre, that employ hierarchical assembly to attain robust material performance, we leveraged matrix-filler interactions within reinforced polymer-peptide hybrids to create self-assembled hydrogels with enhanced properties. Specifically, cellulose nanocrystals (CNCs) were incorporated into peptide-polyurea (PPU) hybrid matrices to tailor key hydrogel features through matrix-filler interactions. Herein, we examined the impact of peptide repeat length and CNC loading on hydrogelation, morphology, mechanics, and thermal behavior of PPU/CNC composite hydrogels. The addition of CNCs into PPU hydrogels resulted in increased gel stiffness; however, the extent of reinforcement of the nanocomposite gels upon nanofiller inclusion also was driven by PPU architecture. Temperature-promoted stiffening transitions observed in nanocomposite PPU hydrogels were dictated by peptide segment length. Analysis of the peptide secondary structure confirmed shifts in the conformation of peptidic domains (α-helices or β-sheets) upon CNC loading. Finally, PPU/CNC hydrogels were probed for their injectability characteristics, demonstrating that nanofiller-matrix interactions were shown to aid rapid network reformation (∼10 s) upon cessation of high shear forces. Overall, this research showcases the potential of modulating matrix-filler interactions within PPU/CNC hydrogels through strategic system design, enabling the tuning of functional hydrogel characteristics for diverse applications.</p>","PeriodicalId":91,"journal":{"name":"Molecular Systems Design & Engineering","volume":" ","pages":""},"PeriodicalIF":3.2000,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12128039/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Molecular Systems Design & Engineering","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1039/d5me00009b","RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, PHYSICAL","Score":null,"Total":0}
引用次数: 0

Abstract

Taking inspiration from natural systems, such as spider silk and mollusk nacre, that employ hierarchical assembly to attain robust material performance, we leveraged matrix-filler interactions within reinforced polymer-peptide hybrids to create self-assembled hydrogels with enhanced properties. Specifically, cellulose nanocrystals (CNCs) were incorporated into peptide-polyurea (PPU) hybrid matrices to tailor key hydrogel features through matrix-filler interactions. Herein, we examined the impact of peptide repeat length and CNC loading on hydrogelation, morphology, mechanics, and thermal behavior of PPU/CNC composite hydrogels. The addition of CNCs into PPU hydrogels resulted in increased gel stiffness; however, the extent of reinforcement of the nanocomposite gels upon nanofiller inclusion also was driven by PPU architecture. Temperature-promoted stiffening transitions observed in nanocomposite PPU hydrogels were dictated by peptide segment length. Analysis of the peptide secondary structure confirmed shifts in the conformation of peptidic domains (α-helices or β-sheets) upon CNC loading. Finally, PPU/CNC hydrogels were probed for their injectability characteristics, demonstrating that nanofiller-matrix interactions were shown to aid rapid network reformation (∼10 s) upon cessation of high shear forces. Overall, this research showcases the potential of modulating matrix-filler interactions within PPU/CNC hydrogels through strategic system design, enabling the tuning of functional hydrogel characteristics for diverse applications.

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
利用肽-纤维素相互作用来定制自组装的可注射水凝胶的性能。
从自然系统(如蜘蛛丝和软体动物珍珠)中获得灵感,采用分层组装来获得强大的材料性能,我们利用增强聚合物-肽杂化物中的基质-填料相互作用来创造具有增强性能的自组装水凝胶。具体来说,纤维素纳米晶体(cnc)被纳入到肽-聚脲(PPU)混合基质中,通过基质-填料相互作用来定制关键的水凝胶特征。在此,我们研究了肽重复长度和CNC负载对PPU/CNC复合水凝胶的水凝胶化、形态、力学和热行为的影响。在PPU水凝胶中加入cnc导致凝胶刚度增加;然而,纳米填料对纳米复合凝胶的增强程度也受PPU结构的影响。在纳米复合PPU水凝胶中观察到的温度促进的硬化转变是由肽段长度决定的。对肽二级结构的分析证实了CNC加载后肽结构域(α-螺旋或β-片)的构象发生了变化。最后,研究人员对PPU/CNC水凝胶的注射特性进行了探测,结果表明,在高剪切力停止后,纳米填料-基质相互作用有助于快速的网络重构(~ 10 s)。总的来说,这项研究展示了通过战略性系统设计来调节PPU/CNC水凝胶中基质-填料相互作用的潜力,使功能水凝胶特性能够适应不同的应用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Molecular Systems Design & Engineering
Molecular Systems Design & Engineering Engineering-Biomedical Engineering
CiteScore
6.40
自引率
2.80%
发文量
144
期刊介绍: Molecular Systems Design & Engineering provides a hub for cutting-edge research into how understanding of molecular properties, behaviour and interactions can be used to design and assemble better materials, systems, and processes to achieve specific functions. These may have applications of technological significance and help address global challenges.
期刊最新文献
Back cover Enhanced thermal response of 3D-printed bilayer hydrogels via nanoclay incorporation. Back cover Harnessing peptide-cellulose interactions to tailor the performance of self-assembled, injectable hydrogels. DynaMate: leveraging AI-agents for customized research workflows
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1