Journal of Asia-pacific Entomology最新文献

Potential of RNAi targeting β-N-acetylglucosaminidase(NAG1)for controlling Dendroctonus valens LeConte (Coleoptera: Scolytidae) 靶向β- n -乙酰氨基葡萄糖苷酶（NAG1）的RNAi控制白桦尺蠖的潜力

IF 1.5 3区农林科学 Q3 ENTOMOLOGY

Journal of Asia-pacific Entomology

Pub Date : 2026-09-01 Epub Date: 2026-08-11 DOI: 10.1016/j.aspen.2026.102608

Yue Sun, Qin Cao, Yong-Hao Nan, Yang-Nan Hou, Chun-Fang Qi, Ji-Gang Li, Jian-Rong Wei

Dendroctonus valens (Coleoptera) is an invasive pest threatening pine forests in China. Long-term chemical application causes pest resistance and environmental pollution, highlighting the urgent need for environmentally friendly control strategies. A chitin degradation-related gene, DvNAG1, was screened based on genomic and transcriptomic data combined with eggNOG and KEGG annotations, and its potential as an RNAi target was evaluated. Phylogenetic analysis classified DvNAG1 (encoding 594 amino acids) into the NAGs I group, clustering with coleopteran homologs. Spatiotemporal expression was examined by RT-qPCR, revealing significantly higher expression in adults than in eggs, and high expression in the Malpighian tubules and gut of fourth-instar larvae, while expression in the head and integument was almost undetectable. The dsRNA/SPc complex was delivered into fourth-instar larvae via integument permeation at 0.7 μg/larva. At this dose, silencing efficiency reached 90%, larval mortality reached 100%, pupation and emergence rates decreased, and pupal/adult malformations were observed. NAG enzyme activity was significantly reduced after DvNAG1 silencing, whereas chitin content increased, suggesting that DvNAG1 affects chitin metabolism and leads to larval death. RNAi results demonstrate that DvNAG1 is essential for growth and development of D. valens larvae. Taken together, these results indicated that silencing DvNAG1 not only increases larval mortality and malformation rates but also disrupts pupation and emergence. Understanding the function of DvNAG1 in D. valens can provide new insights for developing innovative strategies to control D. valens.

松木齿蛾（Coleoptera）是一种威胁中国松林的入侵害虫。长期使用化学品会造成害虫抗性和环境污染，迫切需要环境友好型防治策略。基于基因组和转录组学数据，结合eggNOG和KEGG注释，筛选了一个几丁质降解相关基因DvNAG1，并评估了其作为RNAi靶点的潜力。系统发育分析将编码594个氨基酸的DvNAG1归为NAGs I族，与鞘翼类同源物聚类。通过RT-qPCR检测其时空表达，发现成虫的表达量明显高于卵，在4龄幼虫的马尔比氏小管和肠道中表达量高，而在头部和被皮中几乎检测不到表达。dsRNA/SPc复合物以0.7 μg/只的剂量通过被膜渗透进入4龄幼虫体内。在该剂量下，沉默率达90%，幼虫死亡率达100%，化蛹率和羽化率下降，蛹/成虫畸形。DvNAG1沉默后，NAG酶活性显著降低，而几丁质含量升高，表明DvNAG1影响了几丁质代谢，导致幼虫死亡。RNAi研究结果表明，DvNAG1对丁丁弧菌幼虫的生长发育至关重要。综上所述，这些结果表明，沉默DvNAG1不仅会增加幼虫的死亡率和畸形率，而且会破坏化蛹和羽化。了解DvNAG1基因在缬草属植物中的作用，可以为开发缬草属植物的创新控制策略提供新的见解。

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引用次数: 0

Integrative analyses of lipidomics and transcriptomics provide insights into host lipid regulation by Scleroderma sichuanensis (Hymenoptera: Bethylidae) venom 脂质组学和转录组学的综合分析为四川硬皮病（膜翅目：白茧蜂科）毒液对宿主脂质调控提供了新的思路

IF 1.5 3区农林科学 Q3 ENTOMOLOGY

Journal of Asia-pacific Entomology

Pub Date : 2026-09-01 Epub Date: 2026-08-20 DOI: 10.1016/j.aspen.2026.102614

Long Liu, Hua Yang

Parasitoid wasps comprise an ecologically vital of insects capable of modulating host metabolic processes to benefit the successful development of their progeny. Parasitoid venoms act as maternally produced virulence determinants that are essential for this process. Despite the well-established roles of lipids in membrane organization, energy homeostasis and cellular signaling, the molecular basis of parasitoid venom-induced modulation of host lipid metabolism remains poorly understood. Here, liquid chromatography-mass spectrometry (LC-MS) together with RNA sequencing (RNA-seq) was employed to examine the effects of Scleroderma sichuanensis venom on lipidomic or transcriptome profiles of its host, Tenebrio molitor. Lipidomic analysis showed that S. sichuanensis venom induced dramatic alterations in both the content and composition of host lipid classes. Totally, 251 significantly different lipids were detected, among which glycerophospholipids and glycerolipids were the most abundant, comprising 149 and 65 species, respectively. Notably, venom induced significant accumulation of glycerolipid and membrane remodeling by altering glycerophospholipid composition. Transcriptomic profiling also revealed that venom modulated genes and pathways associated with lipid metabolism, with 327 genes significantly upregulated and 222 genes significantly downregulated, mainly enriched in glycerolipid biosynthesis and glycerophospholipid metabolism. This study provides new evidence for venom-mediated regulation of host lipid metabolism and sheds light on parasitoid-host coevolutionary interactions.

寄生蜂是一种生态上至关重要的昆虫，能够调节宿主的代谢过程，从而有利于其后代的成功发育。拟寄生虫毒液作为母体产生的毒力决定因素，对这一过程至关重要。尽管脂质在膜组织、能量稳态和细胞信号传导中的作用已经确立，但类寄生虫毒液诱导的宿主脂质代谢调节的分子基础仍然知之甚少。本研究采用液相色谱-质谱联用技术（LC-MS）和RNA测序技术（RNA-seq）研究了四川硬皮病毒液对其宿主黄粉虫（tenbrio molitor）脂质组学和转录组学的影响。脂质组学分析表明，川南蛇毒液诱导宿主脂类含量和组成发生显著变化。共检测到251种存在显著差异的脂类，其中甘油磷脂和甘油脂种类最多，分别为149种和65种。值得注意的是，毒液通过改变甘油磷脂组成诱导了甘油脂的显著积累和膜重塑。转录组学分析还发现，毒液调节脂质代谢相关基因和途径，其中327个基因显著上调，222个基因显著下调，主要富集于甘油脂生物合成和甘油磷脂代谢。本研究为毒液介导的宿主脂质代谢调控提供了新的证据，并揭示了拟寄生物与宿主共同进化的相互作用。

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引用次数: 0

Development of an enhanced baculovirus expression vector based on p10 promoter combinations 基于p10启动子组合的增强型杆状病毒表达载体的研制

IF 1.5 3区农林科学 Q3 ENTOMOLOGY

Journal of Asia-pacific Entomology

Pub Date : 2026-09-01 Epub Date: 2026-08-25 DOI: 10.1016/j.aspen.2026.102617

Hyuk-Jin Moon, Hyo-Gyeong Jang, Soo-Dong Woo

The baculovirus expression system (BES) is widely used for recombinant protein production. However, its reliance on very late viral promoters such as p10 and polyhedrin (polh) imposes temporal limitations that can compromise protein yield and quality due to virus-induced cell death. In this study, a novel p10 promoter-based overexpression vector was developed to enhance recombinant protein production while addressing these intrinsic limitations. To enable earlier production and enhance transcriptional activity, additional viral promoters and homologous region (hr) transcriptional enhancers were systematically combined upstream of the p10 promoter. Among the tested constructs, a synergistic combination of the hr5 sequence and the p6.9 promoter significantly enhanced protein expression, resulting in a 1.9-fold increase compared with the conventional p10 promoter alone. These results indicate that the newly developed p10-based overexpression vector is an effective and versatile tool for improving recombinant protein production in BES by enabling higher expression levels.

杆状病毒表达系统（BES）广泛应用于重组蛋白的生产。然而，它依赖于非常晚的病毒启动子，如p10和polyhedrin (polh)，这造成了时间限制，可能由于病毒诱导的细胞死亡而影响蛋白质产量和质量。在这项研究中，我们开发了一种新的基于p10启动子的过表达载体，以提高重组蛋白的产量，同时解决了这些内在的局限性。为了能够更早地产生并增强转录活性，在p10启动子上游系统地结合了额外的病毒启动子和同源区域（hr）转录增强子。在所测试的构建体中，hr5序列与p6.9启动子的协同组合显著增强了蛋白表达，与单独使用常规p10启动子相比，其表达量增加了1.9倍。这些结果表明，新开发的基于p10的过表达载体是一种有效的、通用的工具，可以通过提高表达水平来提高BES中重组蛋白的产量。

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引用次数: 0

Transcriptomic analysis reveals candidate genes associated with egg-laying capacity in two honey bee subspecies (Apis mellifera mellifera and Apis mellifera ligustica) 转录组学分析揭示了两个蜜蜂亚种（Apis mellifera mellifera和Apis mellifera ligustica）产蛋能力相关的候选基因

IF 1.5 3区农林科学 Q3 ENTOMOLOGY

Journal of Asia-pacific Entomology

Pub Date : 2026-09-01 Epub Date: 2026-08-21 DOI: 10.1016/j.aspen.2026.102616

Lu-Xia Pan, Shi-Qing Zhong, Qing-Sheng Niu, Xiang-Shi Huang, Zhi-Yong Liu, Zhi-Jiang Zeng

Apis mellifera ligustica and Apis mellifera mellifera are two important honey bee subspecies widely kept and studied. But their queens differ greatly in egg-laying capacity. To better understand the mechanisms of this difference, ovarian transcriptomic differences between the two subspecies were analyzed using Illumina sequencing technology. A total of 180 common differentially expressed genes (DEGs) were identified, with 61 up-regulated and 119 down-regulated in A. m. ligustica relative to A. m. mellifera queens. Among these, four candidate DEGs related to oocyte maturation, ovarian development, and sperm storage were identified, such as vg, spr, oocyte zinc finger protein and SPATA6. Moreover, candidate genes exhibited alternative splicing divergence between the two subspecies, with more diverse splicing patterns being observed in A. m. ligustica. This study provides valuable gene expression data for elucidating fecundity differences between two honey bee subspecies.

蜜蜂和蜜蜂是被广泛饲养和研究的两个重要的蜜蜂亚种。但它们的蚁后在产卵能力上差别很大。为了更好地了解这种差异的机制，使用Illumina测序技术分析了两个亚种之间的卵巢转录组差异。共鉴定出180个共同差异表达基因（deg），其中ligustica与蜜蜂蜂相比上调61个，下调119个。其中，鉴定出与卵母细胞成熟、卵巢发育和精子储存相关的4个候选deg，分别为vg、spr、卵母细胞锌指蛋白和SPATA6。此外，候选基因在两个亚种之间表现出选择性剪接差异，在A. m. ligustica中观察到更多样化的剪接模式。本研究为阐明两个蜜蜂亚种的繁殖力差异提供了有价值的基因表达数据。

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引用次数: 0

Chemotype-dependent larvicidal activity of clove, cinnamon, and marjoram essential oils against Culex pipiens: biochemical and molecular insights 丁香、肉桂和马郁兰精油对库蚊的化学型依赖性杀幼虫活性：生化和分子的见解

IF 1.3 3区农林科学 Q3 ENTOMOLOGY

Journal of Asia-pacific Entomology

Pub Date : 2026-06-01 Epub Date: 2026-05-27 DOI: 10.1016/j.aspen.2026.102571

Hanan Salman Alyahya , Habeeb M. Al-Solami , Wafa Mohammed Al-Otaibi , Akram S. Alghamdi , Mohammad M. Aljameeli , Ahmed S. Hashem , Jazem A. Mahyoub

Botanical larvicides are increasingly investigated as environmentally compatible alternatives for mosquito control. In the present study, we evaluated the larvicidal activity of clove, cinnamon, and marjoram essential oils against Culex pipiens larvae and linked oil chemotype to biochemical, histopathological, and molecular responses. GC–MS profiling revealed distinct chemotypes dominated by eugenol in clove oil, (E)-cinnamaldehyde in cinnamon oil, and trans-sabinene hydrate in marjoram oil. Across 150–500 ppm, all oils produced concentration-dependent larval mortality, with clove oil showing the highest potency. Regression-derived overall LC₅₀ values were 280.7, 319.4, and 358.5 ppm for clove, marjoram, and cinnamon, respectively, while the corresponding LC₉₀ values were 491.3, 554.6, and 608.5 ppm. Time-course analysis further indicated that clove LC₅₀ declined from approximately 281.3 ppm on day 1 to 233.7 ppm on day 3. Acute knockdown after 30 min followed the same rank order, with clove (≈85.9%) > marjoram (≈73.0%) > cinnamon (≈62.4%), while the solvent control showed no effect. At LC₅₀-equivalent exposure, larvae exhibited marked suppression of GST, AChE, CAT, and SOD activities, accompanied by elevated MDA levels, indicating disruption of detoxification and redox homeostasis. Histopathological analysis revealed clear midgut epithelial damage in treated larvae, with the most severe changes observed after clove-oil exposure. Multivariate analysis separated the oils according to their integrated biological and biochemical effects and identified GST activity as the strongest discriminating variable (canonical loading = − 0.94; canonical r = 0.92; P < 0.001). Consistently, molecular docking supported favorable interactions of major oil constituents within the GST binding pocket, particularly for clove-associated ligands. Collectively, these findings indicate that the larvicidal activity of these essential oils is chemotype-dependent and involves coordinated disruption of detoxification capacity, oxidative balance, and midgut integrity in C. pipiens larvae.

植物性杀幼虫剂作为一种环境相容的蚊虫控制替代品正受到越来越多的研究。在本研究中，我们评估了丁香、肉桂和马郁兰精油对库蚊幼虫的杀幼虫活性，并将精油的化学型与生化、组织病理学和分子反应联系起来。GC-MS分析显示丁香油中的丁香酚、肉桂油中的(E)-肉桂醛和马郁兰油中的水合反式sabinene具有不同的化学型。在150-500 ppm范围内，所有油都产生了浓度依赖性的幼虫死亡率，丁香油的效力最高。丁香、马郁兰和肉桂的总体LC50值分别为280.7、319.4和358.5 ppm，而相应的LC90值分别为491.3、554.6和608.5 ppm。时间过程分析进一步表明丁香LC50从第1天的281.3 ppm下降到第3天的233.7 ppm。30 min后的急性击倒顺序相同，丁香（≈85.9%）&马郁兰（≈73.0%）&桂皮（≈62.4%），而溶剂对照无效果。在lc50等量暴露下，幼虫表现出GST、AChE、CAT和SOD活性的明显抑制，同时MDA水平升高，表明解毒和氧化还原稳态被破坏。组织病理学分析显示，处理过的幼虫中肠上皮明显受损，丁香油暴露后观察到最严重的变化。多变量分析根据其综合生物学和生化效应对油进行分离，并确定GST活性是最强的判别变量（canonical loading = - 0.94; canonical r = 0.92; P < 0.001）。一致地，分子对接支持GST结合口袋内主要油成分的有利相互作用，特别是丁香相关配体。总之，这些发现表明，这些精油的杀幼虫活性是化学型依赖的，并涉及协调破坏库蚊幼虫的解毒能力、氧化平衡和中肠完整性。

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引用次数: 0

Expression and functional analysis of glutathione S-transferase gene GmGST2a different tissues of from Galleria mellonella larvae 谷胱甘肽s -转移酶基因GmGST2a在不同组织中表达及功能分析

IF 1.3 3区农林科学 Q3 ENTOMOLOGY

Journal of Asia-pacific Entomology

Pub Date : 2026-06-01 Epub Date: 2026-05-28 DOI: 10.1016/j.aspen.2026.102574

Yangyang Liu, Ziliang Zheng, Xuyu Ran, Jiajuan Shen, Shengfeng Liu, Yingying Li, Guoyong Li

Galleria mellonella poses a significant threat to Apis cerana cerana colonies, causing economic losses in apiculture. However, the overuse of chemical insecticides to control this pest has led to pesticide resistance and environmental hazards. To explore eco-friendly control strategies, we cloned and identified the glutathione S-transferase gene GmGST2a (GenBank PX578457) from G. mellonella. The full-length cDNA is 1059 bp with a 615 bp ORF encoding a 204 amino acids protein. Phylogenetic analysis revealed that GmGST2a clustered with the orthologue from Aphomia sociella. RT-qPCR analysis showed that its expression was significantly higher in the larval stage than in the pupal and adult stages (p < 0.05). Tissue-specific expression analysis showed that transcript levels in the foregut and midgut were 8.2- and 9.8-fold higher, respectively, than in the larval epidermis. Following treatment with 30 mg/L chlorantraniliprole, GmGST2a expression increased 4.5-fold compared to the control (p < 0.01). Meanwhile, expression levels in the 50 mg/L treatment group were similar to those of the control group (approximately 1.0-fold). RNAi-mediated knockdown of GmGST2a resulted in a 50% reduction in gene expression within 12 h, accompanied by a 60% decrease in GST enzyme activity. This suppression led to almost 100% mortality or developmental abnormalities, demonstrating that silencing GmGST2a significantly compromises detoxification capacity and increases lethality in G. mellonella. In conclusion, GmGST2a is a promising molecular target for controlling G. mellonella. These findings highlight the potential of developing eco-friendly, RNAi-based pesticides to support sustainable pest management strategies in apiculture.

mellonella对中华蜜蜂（Apis cerana cerana）的种群构成严重威胁，给养蜂业造成经济损失。然而，过度使用化学杀虫剂来控制这种害虫已经导致了农药抗药性和环境危害。为了探索绿色防治策略，我们克隆并鉴定了谷胱甘肽s -转移酶基因GmGST2a （GenBank PX578457）。cDNA全长1059 bp， ORF全长615 bp，编码204个氨基酸的蛋白。系统发育分析显示，GmGST2a与来自社会恐蝇的同源基因聚类。RT-qPCR分析显示，其在幼虫期的表达量显著高于蛹期和成虫期（p < 0.05）。组织特异性表达分析显示，前肠和中肠的转录物水平分别比幼虫表皮高8.2倍和9.8倍。30 mg/L氯虫腈处理后，GmGST2a表达量较对照组增加4.5倍（p < 0.01）。同时，50 mg/L处理组的表达水平与对照组相似（约为1.0倍）。rnai介导的GmGST2a敲低导致基因表达在12小时内降低50%，同时GST酶活性降低60%。这种抑制导致几乎100%的死亡率或发育异常，这表明沉默GmGST2a显着降低了脱毒能力并增加了大麦香杆菌的致死率。综上所述，GmGST2a是一个很有前途的分子靶点。这些发现强调了开发基于rnai的生态友好型农药的潜力，以支持养蜂业的可持续虫害管理战略。

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引用次数: 0

Unlocking the insecticidal potential of a underexplored dryland biome: fixed oil and hydrolate of Croton heliotropiifolius against the maize weevil 释放未开发的旱地生物群系的杀虫潜力：巴豆的固定油和水合物对玉米象鼻虫的作用

IF 1.3 3区农林科学 Q3 ENTOMOLOGY

Journal of Asia-pacific Entomology

Pub Date : 2026-06-01 DOI: 10.1016/j.aspen.2026.102576

Jose Nnehanderson Freitas da Silva, João Paulo Ramos de Melo, Gabriela Fabrizia Diniz Leite, João Carlos Dias dos Santos, Bruno Henrique dos Santos Silva, Jorge Luiz da Silva, José Jêmesson Gomes da Silva, Beatriz de Lima Santos, Maria Victoria Bezerra Batista, Gabriela Alves da Costa, César Auguste Badji

The maize weevil (Sitophilus zeamais) is one of the most damaging pests of stored grains worldwide, and its control relies heavily on synthetic insecticides, which have led to resistance development and environmental concerns. Identifying innovative, plant-derived alternatives is therefore a priority for sustainable pest management. In this context, the Brazilian Caatinga biome, one of the largest seasonally dry tropical forests in the world and still poorly explored for bioactive compounds, represents a valuable source for the discovery of novel biopesticides. This study investigated, the insecticidal potential and behavioral effects of fixed oil and hydrolate derived from Croton heliotropiifolius leaves against adult S. zeamais. Chemical characterization revealed that the fixed oil was dominated by butanoic acid (32.75%) and methyl N-methylanthranilate (32.58%), while the hydrolate was mainly composed of methyl N-methylanthranilate (25.56%) and linalool (14.65%). Both bioproducts exhibited significant toxicity and strong sublethal effects, including reduced food exploration and marked repellency (repellency index = 0.65–0.70). These findings demonstrate that C. heliotropiifolius, a native Caatinga species, is a promising source of innovative bioproducts, combining toxic and behavioral modes of action. Our results highlight the untapped biotechnological potential of Caatinga flora and support the development of plant-based biopesticides as sustainable tools for integrated pest management in stored grains.

玉米象甲（Sitophilus zeamais）是世界范围内储存谷物中最具破坏性的害虫之一，其控制严重依赖于合成杀虫剂，这导致了抗性的发展和环境问题。因此，确定创新的植物源替代品是可持续虫害管理的优先事项。在这种情况下，巴西的Caatinga生物群落是世界上最大的季节性干燥热带森林之一，对生物活性化合物的探索仍然很少，它代表了发现新型生物农药的宝贵来源。研究了巴Croton heliotropiifolius叶片固定油和固定水合物对玉米螟成虫的杀虫潜力和行为效果。化学表征表明，固定油主要由丁酸（32.75%）和n -甲基氰胺酸甲酯（32.58%）组成，而水解产物主要由n -甲基氰胺酸甲酯（25.56%）和芳樟醇（14.65%）组成。两种生物制品均表现出明显的毒性和强亚致死效应，包括减少食物探索和明显的驱避（驱避指数= 0.65-0.70）。这些发现表明，C. heliotropiifolius是一种结合毒性和行为作用模式的创新生物制品。我们的研究结果突出了Caatinga植物群尚未开发的生物技术潜力，并支持开发基于植物的生物农药作为储粮害虫综合治理的可持续工具。

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引用次数: 0

Is there a three-instar pathway in the development of the Japanese pine sawyer? A revised hypothesis for the life cycle 日本松柏的发展是否存在三期路径？生命周期的修正假设

IF 1.3 3区农林科学 Q3 ENTOMOLOGY

Journal of Asia-pacific Entomology

Pub Date : 2026-03-01 Epub Date: 2026-02-21 DOI: 10.1016/j.aspen.2026.102537

Su Bin Kim , Jin Woo Heo , Dong-Soon Kim

The Japanese pine sawyer (JPS), Monochamus alternatus, is the primary vector of the pine wood nematode, the causal agent of pine wilt disease. Despite more than five decades of intensive research, its complete life cycle—particularly the developmental pathways defined by larval instar number prior to pupation—remains incompletely resolved. This study reexamines JPS’s life cycle from the perspective of instar-pathway (IP) selection. While four- and five-instar pathways (4-IP and 5-IP) have been documented, empirical support for a three-instar pathway (3-IP) has remained inconclusive. We tracked the full developmental trajectories of individually marked larvae from egg hatch to pupal chamber construction under near-natural field conditions. Special attention was given to tunnel-digging behavior, a characteristic sequence of boring and entrance-hole plugging that precedes overwintering. Our observations indicate that the initiation of tunnel-digging behavior coincides with the completion of IP selection. All individuals committed to an IP only after reaching at least the fourth instar, most frequently during the mid- to late fourth instar stage. No explicit evidence was found for pupation or adult emergence following a 3-IP. Reanalysis of previously published datasets likewise failed to provide convincing support for a 3-IP. These results suggest that the fourth instar represents a critical developmental threshold for progression toward pupation and adult emergence. Variation beyond this threshold allows some individuals to continue growth into a fifth instar, resulting in alternative 5-IP. The present study redefines JPS’s developmental framework and provides an improved foundation for future modeling of its phenology and population dynamics.

松材线虫是松材线虫的主要传播媒介，而松材线虫是松材枯萎病的致病因子。尽管经过了50多年的深入研究，但其完整的生命周期，特别是由蛹化前的幼虫龄数所定义的发育途径，仍未完全解决。本研究从instar-pathway （IP）选择的角度重新审视了JPS的生命周期。虽然已有文献记载了4-IP和5-IP信号通路，但3-IP信号通路的实证支持仍然没有定论。我们在接近自然的野外条件下，追踪了个体标记幼虫从卵孵化到蛹室形成的完整发育轨迹。特别关注隧道开挖行为，即在越冬之前的钻孔和入口孔堵塞的特征序列。我们的观察表明，隧道掘进行为的开始与IP选择的完成是一致的。所有的个人都是在达到至少第4个阶段之后才开始投入IP，最常见的是在第4个阶段的中后期。没有明确的证据表明3-IP后会出现化蛹或成虫羽化。对先前发布的数据集的重新分析同样未能为3-IP提供令人信服的支持。这些结果表明，四龄代表了向化蛹和成虫羽化发展的关键发育门槛。超过这个阈值的变异允许一些个体继续生长到第五阶段，从而产生替代的5-IP。本研究重新定义了JPS的发育框架，并为其物候和种群动态的未来建模提供了改进的基础。

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引用次数: 0

Efficacy of Orius strigicollis (Poppius) (Hemiptera: Anthocoridae) with extended patch residence time by artificial selection against Frankliniella intonsa (Trybom) (Thysanoptera: Thripidae) in greenhouse strawberries 延长斑块停留时间人工选择对大棚草莓横纹螟（半翅目：蠓科）的防治效果

IF 1.3 3区农林科学 Q3 ENTOMOLOGY

Journal of Asia-pacific Entomology

Pub Date : 2026-03-01 Epub Date: 2026-02-02 DOI: 10.1016/j.aspen.2026.102528

Tomoya Katsuno, Tomokazu Seko, Aki Sagisaka, Ritsuko Murakami

Thrips are major pests in greenhouse strawberries, and their management is increasingly difficult due to the development of resistance to chemical insecticides. Biological control agents represent a promising alternative. In Japan, the predatory bug Orius strigicollis is commercially available for thrips control, but its poor establishment under low prey density in strawberry crops has limited its effectiveness. To address this, selected lines of O. strigicollis with reduced walking activity and extended patch residence time were developed through artificial selection. While these selected lines showed significant performance in eggplants, their efficacy in strawberries remained unclear. In this study, we evaluated their performance in strawberries against Frankliniella intonsa. Laboratory predation assays revealed no significant difference in thrips consumption between selected and wild-type lines on a strawberry flower. In three-year greenhouse trials, the selected lines exhibited improved establishment, resulting in higher numbers of adults and nymphs and thrips suppression compared to wild-type lines. Orius strigicollis nymphs overlapped spatially with thrips larvae in young green fruits, which may have contributed to significant suppression of F. intonsa populations. These findings suggest that artificial selection for extended patch residence time can enhance the efficacy of O. strigicollis as a biological control agent in greenhouse strawberry cultivation. Combining selected lines with other management techniques, such as predatory mites or supplementary food, may further improve thrips control.

蓟马是温室草莓的主要害虫，由于对化学杀虫剂的抗性发展，蓟马的管理越来越困难。生物防治剂是一种很有前途的替代方法。在日本，捕食性臭虫Orius strigicollis在商业上可用于控制蓟马，但在草莓作物中猎物密度低的情况下，其建立不良限制了其效果。为了解决这一问题，通过人工选择，选择了行走活动减少、斑块停留时间延长的疏螺旋藻株系。虽然这些选育的品系在茄子上表现显著，但它们在草莓上的效果尚不清楚。在本研究中，我们评价了它们在草莓中的抗蚜性能。实验室捕食分析显示，在草莓花上，蓟马的食用量在选择系和野生系之间没有显著差异。在为期三年的温室试验中，所选品系表现出较野生型品系更高的成虫和若虫数量以及对蓟马的抑制。鲜绿果实中，鸢尾若虫与蓟马幼虫在空间上重叠，这可能是抑制鸢尾若虫种群的重要原因。上述结果表明，通过人工选择延长田间停留时间，可以提高鸢尾弧菌在温室草莓栽培中的防效。将选定品系与其他管理技术（如捕食性螨或补充食物）相结合，可以进一步改善对蓟马的控制。

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引用次数: 0

Haplotype screening of the pinewood Nematode, Bursaphelenchus xylophilus Cultures in South Korea 韩国松材线虫培养物的单倍型筛选

IF 1.3 3区农林科学 Q3 ENTOMOLOGY

Journal of Asia-pacific Entomology

Pub Date : 2026-03-01 Epub Date: 2026-01-29 DOI: 10.1016/j.aspen.2026.102526

Hyeon Yoo , Seunghyun Lee , Jinbae Seung , Jounga Son , Min-Jung Kim , Seunghwan Lee

The pinewood nematode (PWN), Bursaphelenchus xylophilus, was introduced to South Korea in the 1980 s and initially caused pine wilt disease (PWD) primarily in Pinus densiflora and Pinus thunbergii in the southern regions. The first infestation in Pinus koraiensis was reported in 2008, and severe outbreaks have recently been observed in P. koraiensis stands in the central regions of Korea. To examine whether this new outbreak pattern reflects the presence of distinct haplotypes, we analyzed sequences from 25 PWN isolates maintained through successive breeding. We sequenced three loci: mitochondrial cytochrome b (cytb), the nuclear protein-coding gene cellulase, and the ribosomal DNA internal transcribed spacer (ITS). These regions were selected to maximize the utility of publicly available sequences for comparison. Both cytb and ITS sequences showed complete genetic identity. In contrast, cellulase sequences exhibited five haplotypes differing by up to three nucleotide substitutions, but geographic or host-associated patterns were not detected. These results suggest that the recent PWN outbreak is not attributable to the emergence of a novel haplotype of PWN but may instead be driven by other ecological or environmental factors that have not yet been identified.

松材线虫（Bursaphelenchus xylophilus）于20世纪80年代传入韩国，最初主要在南部地区的密松（Pinus densiflora）和松（Pinus thunbergii）中引起松树枯萎病（PWD）。2008年报告了红松的第一次侵染，最近在韩国中部地区的红松林分中观察到严重的疫情。为了检验这种新的暴发模式是否反映了不同单倍型的存在，我们分析了通过连续育种维持的25株PWN分离株的序列。我们对三个位点进行了测序：线粒体细胞色素b （cytb）、核蛋白编码基因纤维素酶和核糖体DNA内转录间隔区（ITS）。选择这些区域是为了最大限度地利用公开可用的序列进行比较。cytb和ITS序列具有完全的遗传同源性。相比之下，纤维素酶序列表现出五种单倍型，最多有三个核苷酸替换，但没有检测到地理或宿主相关的模式。这些结果表明，最近的PWN暴发不能归因于PWN新单倍型的出现，而是可能由尚未确定的其他生态或环境因素驱动。

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引用次数: 0