Journal of Oral Pathology & Medicine最新文献

Long-Term Care of OLP Patients: A Retrospective Study From a Single Hospital Outpatient Clinic. OLP患者的长期护理：来自一家医院门诊的回顾性研究。

IF 2.3 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Oral Pathology & Medicine

Pub Date : 2026-09-04 DOI: 10.1111/jop.70179

F Scilla, G Gioco, L Ferrero, R Castagnola, A Zafarone, L Cimino, M Tranfa, C Rupe, C Lajolo

Background: Oral lichen planus (OLP) is a chronic immune-mediated disorder with malignant potential. However, its real-world clinical course remains incompletely characterized. This study aimed to describe the natural clinical-histopathological trajectory of OLP in a single tertiary centre and to identify factors associated with dysplasia and malignant transformation over time.

Methods: We conducted a single-centre retrospective cohort study including patients diagnosed with OLP and followed between 2007 and 2024 at the Oral Medicine Unit of Fondazione Policlinico Universitario A. Gemelli IRCCS. Demographic, clinical (red vs. white phenotype and lesion distribution), microbiological (Candida spp.), therapeutic, and histopathological variables were collected across follow-up. Univariate and multivariable logistic regression models were used to evaluate factors associated with dysplasia and malignant transformation.

Results: Among 308 screened patients, 246 met inclusion criteria (173 women, 70.3%; mean age 63.2 ± 15.0 years) with a mean follow-up of 55.5 ± 37.0 months. At baseline, Candida spp. colonization was detected in 55 patients (22.4%) and was more frequent in red-type OLP than white-type lesions (33.3% vs. 17.0%; OR 2.44, 95% CI 1.32-4.53; p = 0.005). During follow-up, 33 patients (13.4%) developed fungal infection despite an initially negative swab. Epithelial dysplasia emerged in 42 patients (17.1%). In multivariable analysis, dysplasia was independently associated with increasing age (OR 1.06, 95% CI 1.03-1.10; p = 0.0001) and red-type OLP(OR 2.24, 95% CI 1.10-4.55; p = 0.026). Eight patients (3.25%) developed infiltrative OSCC after a mean interval of 28.3 months from OLP diagnosis (range 4-55).

Conclusions: In this long-term single-centre cohort, OLP frequently evolved through dysplasia during follow-up, and malignant transformation occurred in a minority of patients. Older age and red-type phenotype characterized patients more likely to develop dysplasia, while malignant transformation was more frequent in the case of previously documented dysplastic lesions, in keeping with the notion of dysplasia as a step along the carcinogenic pathway, older age, and diffuse lesions.

背景：口腔扁平苔藓（OLP）是一种具有恶性潜能的慢性免疫介导疾病。然而，其现实世界的临床过程仍然不完全表征。本研究旨在描述OLP在单一三级中心的自然临床-组织病理学轨迹，并确定与发育不良和恶性转化相关的因素。方法：我们在Fondazione Policlinico Universitario a . Gemelli IRCCS口腔医学部门进行了一项单中心回顾性队列研究，包括2007年至2024年间诊断为OLP的患者。在随访期间收集人口统计学、临床（红色与白色表型和病变分布）、微生物学（念珠菌）、治疗和组织病理学变量。单变量和多变量logistic回归模型用于评估与不典型增生和恶性转化相关的因素。结果：308例患者中，246例符合纳入标准（女性173例，70.3%，平均年龄63.2±15.0岁），平均随访时间55.5±37.0个月。基线时，55例患者（22.4%）检测到念珠菌定植，红色型OLP比白色型病变更常见（33.3%比17.0%;OR 2.44, 95% CI 1.32-4.53; p = 0.005）。在随访期间，33名患者（13.4%）出现真菌感染，尽管最初拭子呈阴性。42例（17.1%）出现上皮发育不良。在多变量分析中，发育不良与年龄增加（OR 1.06, 95% CI 1.03-1.10; p = 0.0001）和红色型OLP（OR 2.24, 95% CI 1.10-4.55; p = 0.026）独立相关。8例（3.25%）患者在OLP诊断后平均间隔28.3个月（范围4-55）发展为浸润性OSCC。结论：在这个长期的单中心队列中，OLP在随访期间经常因发育不良而发展，少数患者发生恶性转化。年龄较大和红型表型的患者更容易发生发育不良，而在先前记录的发育不良病变中，恶性转化更频繁，这与发育不良作为致癌途径的一个步骤、年龄较大和弥漫性病变的概念相一致。

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引用次数: 0

Inhibition of Aurora Kinase A Prevents Malignant Transformation in Oral Leukoplakia. 抑制极光激酶A可预防口腔白斑的恶性转化。

IF 2.3 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Oral Pathology & Medicine

Pub Date : 2026-09-01 DOI: 10.1111/jop.70177

Fengying Yin, Mengjun Li, Yi Xu, Chuan Xin, Yining Li, Qianming Chen

Background: Aurora Kinase A (AURKA), a highly conserved and potent kinase, is frequently overexpressed in diverse cancers. However, its role in the malignant transformation (MT) of oral mucosa remains poorly understood. This study investigated the expression pattern of AURKA and evaluated the therapeutic potential of its inhibitor in oral leukoplakia (OLK), the most representative oral potentially malignant disorder.

Methods: Immunohistochemistry was performed on clinical samples (21 normal controls, 27 OLK, 23 MT of OLK) to examine AURKA expression and its correlation with clinicopathological characteristics. The inhibitory effect of targeting AURKA on tumor proliferation was verified in vitro using two oral squamous cell carcinoma (OSCC) cell lines. Additionally, the chemopreventive effect of the AURKA inhibitor Alisertib on MT was assessed using a 4-nitroquinoline oxide (4NQO)-induced mouse OLK model.

Results: AURKA expression levels positively correlated with the malignant progression of OLK. Significantly higher AURKA expression percentages were observed in non-homogeneous lesions (10.39% ± 4.28% vs. 3.53% ± 4.22% homogeneous, p < 0.001), lesions ≥ 2 cm² (11.19% ± 4.55% vs. 4.69% ± 4.36% < 2 cm², p < 0.01), and lesions with high-risk dysplasia (12.10% ± 4.04% vs. 4.59% ± 4.12% low-risk dysplasia, p < 0.001). Targeting AURKA significantly inhibited the proliferation of OSCC cells in vitro. Furthermore, Alisertib treatment effectively suppressed oral mucosa carcinogenesis in the 4NQO model.

Conclusion: Increased AURKA expression may represent an early molecular event in OLK carcinogenesis, and targeting AURKA with alisertib is a candidate for potential applications in the management of OLK.

背景：极光激酶A （AURKA）是一种高度保守和有效的激酶，在多种癌症中经常过表达。然而，其在口腔黏膜恶性转化（MT）中的作用仍然知之甚少。本研究研究了AURKA的表达模式，并评估了其抑制剂对口腔白斑（OLK）的治疗潜力，口腔白斑是最具代表性的口腔潜在恶性疾病。方法：采用免疫组化方法对临床标本（正常对照21例，OLK 27例，OLK 23 MT）检测AURKA表达及其与临床病理特征的相关性。利用两株口腔鳞状细胞癌（OSCC）细胞株，体外验证了靶向AURKA对肿瘤增殖的抑制作用。此外，利用4-硝基喹啉氧化物（4NQO）诱导的小鼠OLK模型，评估AURKA抑制剂Alisertib对MT的化学预防作用。结果：AURKA表达水平与OLK恶性进展呈正相关。在非均匀病变中，AURKA的表达率显著高于非均匀病变(10.39%±4.28% vs. 3.53%±4.22%,p 2(11.19%±4.55% vs. 4.69%±4.36%)2，p结论：AURKA表达升高可能代表OLK癌变的早期分子事件，alisertib靶向AURKA是OLK治疗的潜在应用候选。

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引用次数: 0

Tempol Protects Against Radiation Injury in the Submandibular Gland Through the β-Catenin Signaling Pathway Tempol通过β-Catenin信号通路保护颌下腺免受辐射损伤。

IF 2.3 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Oral Pathology & Medicine

Pub Date : 2026-09-01 Epub Date: 2026-05-14 DOI: 10.1111/jop.70147

Jinfeng Wang, Mingjun Xu, Jinghua Zhong, Kui Zhong, Zheng Chen, Chunyu Liu, Chao Liu

Background

Radiation damage severely impacts salivary gland function and cell survival, especially in head and neck radiotherapy. Tempol (TPL), a free radical scavenger, has shown protective effects against radiation damage. This research aimed to investigate the protective effects of TPL on radiation-induced damage in Hs917.T cells and the submandibular gland (SMG) of C57BL/6 mice, along with the mechanisms involved.

Methods

Human parotid fibroblasts (Hs917.T) were pre-treated with TPL and exposed to ionizing radiation (IR). Protective effects were evaluated using MTT, clonogenic survival assays, flow cytometry, and intracellular reactive oxygen species levels. In vivo, C57BL/6 mice were pre-treated with TPL (275 mg/kg) and exposed to 15 gray (Gy). Effects were assessed by survival rates, body weight changes, histological analysis, and TUNEL staining. Changes in apoptosis-related markers and β-catenin signaling pathway were analyzed, and the role of TPL was verified using the β-catenin inhibitor XAV939.

Results

TPL pre-treatment increased cell survival, reduced apoptosis, alleviated cell cycle arrest, and decreased intracellular superoxide and hydrogen peroxide levels in Hs917. T cells. In C57BL/6 mice, pre-treatment with TPL improved survival, mitigated weight loss, reduced SMG damage, and decreased apoptosis. TPL inhibited IR-induced apoptosis by increasing Bcl-2 expression and decreasing Bax and caspase-9 levels. TPL exerted anti-apoptotic and protective effects by upregulating the expression of β-catenin, promoting its nuclear translocation, and inhibiting its phosphorylation. These protective effects of TPL were reversed by XAV939.

Conclusions

TPL exerted protective effects against IR-induced damage in Hs917.T cells and the SMG of C57BL/6 mice through activating the β-catenin signaling pathway, inhibiting cell apoptosis, and alleviating oxidative stress.

背景：放射损伤严重影响唾液腺功能和细胞存活，尤其是头颈部放射治疗。天门酚（TPL）是一种自由基清除剂，对辐射损伤具有保护作用。本研究旨在探讨TPL对Hs917辐射损伤的保护作用。T细胞和C57BL/6小鼠的下颌下腺（SMG），以及相关机制。方法：用TPL预处理人腮腺成纤维细胞（Hs917.T），电离辐射（IR）照射。通过MTT、克隆生存测定、流式细胞术和细胞内活性氧水平来评估保护作用。在体内，C57BL/6小鼠接受TPL （275 mg/kg）预处理，暴露于15 Gy （Gy）。通过生存率、体重变化、组织学分析和TUNEL染色来评估效果。分析凋亡相关标志物和β-catenin信号通路的变化，并利用β-catenin抑制剂XAV939验证TPL的作用。结果：TPL预处理可提高Hs917细胞存活率，减少细胞凋亡，减轻细胞周期阻滞，降低细胞内超氧化物和过氧化氢水平。T细胞。在C57BL/6小鼠中，TPL预处理提高了存活率，减轻了体重减轻，减轻了SMG损伤，减少了细胞凋亡。TPL通过提高Bcl-2表达、降低Bax和caspase-9水平抑制ir诱导的细胞凋亡。TPL通过上调β-catenin的表达，促进其核易位，抑制其磷酸化，发挥抗凋亡和保护作用。TPL的这些保护作用被XAV939逆转。结论：TPL对ir致Hs917损伤具有保护作用。通过激活β-catenin信号通路，抑制细胞凋亡，减轻氧化应激，对C57BL/6小鼠T细胞和SMG的影响。

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引用次数: 0

Heterogeneity of miRNAs in Salivary Gland Neoplasms: An Exploratory Pilot Study 唾液腺肿瘤中mirna的异质性：一项探索性初步研究。

IF 2.3 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Oral Pathology & Medicine

Pub Date : 2026-09-01 Epub Date: 2026-05-25 DOI: 10.1111/jop.70150

Dandara Andrade de Santana, Poliana Ramos Braga, Daniel Araki Ribeiro, Patricia Ramos Cury, Ana Carolina Velasco Pondé de Sena, Flávia Caló de Aquino Xavier, Fernando Augusto Soares, Iguaracyra Barreto de Araújo, Fabio Albuquerque Marchi, Valéria Souza Freitas, Cláudia Malheiros Coutinho-Camillo, Jean Nunes dos Santos

Introduction

MicroRNAs (miRNAs) are small RNAs that have been associated with tumourigenesis and tumour progression and that play an important role in the pathogenesis of salivary gland neoplasms. The aim of this study was to evaluate the global miRNA expression profile in a sample of salivary gland neoplasms.

Methods

Cases of pleomorphic adenoma (n = 4), adenoid cystic carcinoma (n = 4) and mucoepidermoid carcinoma (n = 4) were studied by real-time RT-PCR.

Results

The main up-regulated miRNAs were hsa-miR-373, hsa-miR-675, hsa-miR-508, hsa-miR-1290 and hsa-miR−483-3p, while hsa-miR-105 and hsa-let-7f-1 were among the most down-regulated miRNAs compared to the normal salivary gland. Hsa-miR-106b, hsa-miR-142-5p, hsa-miR-622, hsa-miR-642, hsa-miR-574-3p and hsa-miR-486 were significantly differentially expressed (p < 0.05) when each neoplasm and normal glandular tissue were compared.

Conclusion

This study described the global molecular signature of miRNAs in benign and malignant salivary gland neoplasms and provided preliminary evidence of miRNA dysregulation, suggesting a potential association between specific miRNA signatures and molecular pathways implicated in tumour progression.

简介：MicroRNAs （miRNAs）是一种与肿瘤发生和肿瘤进展相关的小rna，在唾液腺肿瘤的发病机制中起着重要作用。本研究的目的是评估唾液腺肿瘤样本中的全局miRNA表达谱。方法：采用实时荧光定量pcr技术对4例多形性腺瘤、4例腺样囊性癌和4例粘液表皮样癌进行分析。结果：与正常唾液腺相比，hsa-miR-373、hsa-miR-675、hsa-miR-508、hsa-miR-1290和hsa-miR-483-3p是主要上调的miRNAs，而hsa-miR-105和hsa-let-7f-1是下调最多的miRNAs。Hsa-miR-106b、hsa-miR-142-5p、hsa-miR-622、hsa-miR-642、hsa-miR-574-3p和hsa-miR-486的表达存在显著差异(p)结论：本研究描述了良性和恶性唾液腺肿瘤中miRNA的全局分子特征，并提供了miRNA失调的初步证据，表明特异性miRNA特征与肿瘤进展相关的分子途径之间存在潜在关联。

{"title":"Heterogeneity of miRNAs in Salivary Gland Neoplasms: An Exploratory Pilot Study","authors":"Dandara Andrade de Santana, Poliana Ramos Braga, Daniel Araki Ribeiro, Patricia Ramos Cury, Ana Carolina Velasco Pondé de Sena, Flávia Caló de Aquino Xavier, Fernando Augusto Soares, Iguaracyra Barreto de Araújo, Fabio Albuquerque Marchi, Valéria Souza Freitas, Cláudia Malheiros Coutinho-Camillo, Jean Nunes dos Santos","doi":"10.1111/jop.70150","DOIUrl":"10.1111/jop.70150","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Introduction</h3>\u0000 \u0000 <p>MicroRNAs (miRNAs) are small RNAs that have been associated with tumourigenesis and tumour progression and that play an important role in the pathogenesis of salivary gland neoplasms. The aim of this study was to evaluate the global miRNA expression profile in a sample of salivary gland neoplasms.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Cases of pleomorphic adenoma (<i>n</i> = 4), adenoid cystic carcinoma (<i>n</i> = 4) and mucoepidermoid carcinoma (<i>n</i> = 4) were studied by real-time RT-PCR.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The main up-regulated miRNAs were hsa-miR-373, hsa-miR-675, hsa-miR-508, hsa-miR-1290 and hsa-miR−483-3p, while hsa-miR-105 and hsa-let-7f-1 were among the most down-regulated miRNAs compared to the normal salivary gland. Hsa-miR-106b, hsa-miR-142-5p, hsa-miR-622, hsa-miR-642, hsa-miR-574-3p and hsa-miR-486 were significantly differentially expressed (<i>p</i> < 0.05) when each neoplasm and normal glandular tissue were compared.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>This study described the global molecular signature of miRNAs in benign and malignant salivary gland neoplasms and provided preliminary evidence of miRNA dysregulation, suggesting a potential association between specific miRNA signatures and molecular pathways implicated in tumour progression.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":"55 8","pages":"906-916"},"PeriodicalIF":2.3,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jop.70150","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148016146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pg-Induced ATR Activation Promotes ESCC Progression via M2 TAM Polarization pg诱导的ATR激活通过M2 TAM极化促进ESCC进展。

IF 2.3 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Oral Pathology & Medicine

Pub Date : 2026-09-01 Epub Date: 2026-06-04 DOI: 10.1111/jop.70157

Yanhua Sun, Lin Yang, Xiaodong Wei

<div> <section> <h3> Background</h3> <p>Emerging evidence suggests that oral pathogens may contribute to the development of systemic malignancies. <i>Porphyromonas gingivalis</i> (Pg), a major periodontal pathogen, has been implicated in several cancers including esophageal squamous cell carcinoma (ESCC). However, the molecular mechanisms underlying this association remain unclear.</p> </section> <section> <h3> Objective</h3> <p>This study aimed to investigate the impact of <i>Porphyromonas gingivalis</i> (Pg) on the growth of esophageal squamous cell carcinoma (ESCC) and its potential mechanisms.</p> </section> <section> <h3> Methods</h3> <p>THP-1 cells were differentiated into M0 macrophages with PMA and divided into control group, Pg group, and Pg + siATR group. THP-1 cells were divided into three groups: control group, Pg group, and Pg + siATR group. The control and Pg groups were transfected with siNC, while the Pg + siATR group was transfected with siATR. After transfection, the Pg group and Pg + siATR group were incubated with 200 MOI pg. The control group was cultured normally. Cells and supernatants were collected, and macrophage polarization status was detected with qRT-PCR, Western blot, and flow cytometry. Macrophages treated differently were co-cultured with human esophageal squamous cell carcinoma cell line KYSE150. The proliferation, invasion, and apoptosis of KYSE150 were examined. KYSE150+shATR and KYSE150+shNC cell lines were constructed with a lentivirus system. Thirty male BALB/c mice aged 6–8 weeks were randomly divided into control group, pg group, and pg+shATR group, with 10 mice in each group. For the Pg and Pg+shATR groups, 200 μL Pg (1 × 10<sup>7</sup> CFU/mouse) was applied to the mandibular molars of mice four times a week for 1 month. The control group was treated with the vehicle (CMC) only. After 3 weeks of bacterial colonization, KYSE150+shATR cells (1 × 10<sup>6</sup> cells/mouse) were inoculated into the right axilla of mice in the Pg+shATR group, while KYSE150 + shNC (1 × 10<sup>6</sup> cells/mouse) were inoculated into the right axilla of mice in the control and Pg groups. Tumor volume was measured with calipers every 7 days. After 4 weeks of experimentation, D-luciferin potassium salt was injected intraperitoneally at 150 mg/kg, and bioluminescence imaging was performed after sodium pentobarbital anesthesia. The mice were euthanized post-imaging, and macrophage polarization in tumor tissues was examined with qRT-PCR, histopathological examination, and Western blot.</p> </section> <section> <h3> Results</h3> <p>In this study, we fou

背景：越来越多的证据表明，口腔病原体可能有助于全身性恶性肿瘤的发展。牙龈卟啉单胞菌（Pg）是一种主要的牙周病原体，与包括食管鳞状细胞癌（ESCC）在内的几种癌症有关。然而，这种关联的分子机制尚不清楚。目的：探讨牙龈卟啉单胞菌（Porphyromonas gingivalis, Pg）对食管鳞状细胞癌（ESCC）生长的影响及其潜在机制。方法：用PMA将THP-1细胞分化为M0巨噬细胞，分为对照组、Pg组和Pg + siATR组。将THP-1细胞分为3组：对照组、Pg组、Pg + siATR组。对照组和Pg组分别转染siNC， Pg + siATR组转染siATR。转染后，Pg组和Pg + siATR组用200 MOI Pg孵育，对照组正常培养。收集细胞及上清液，采用qRT-PCR、Western blot、流式细胞术检测巨噬细胞极化状态。不同处理的巨噬细胞与人食管鳞状细胞癌细胞系KYSE150共培养。检测KYSE150的增殖、侵袭和凋亡情况。用慢病毒系统构建KYSE150+shATR和KYSE150+shNC细胞系。选取6 ~ 8周龄雄性BALB/c小鼠30只，随机分为对照组、pg组和pg+shATR组，每组10只。Pg和Pg+shATR组小鼠下颌骨磨牙注射200 μL Pg （1 × 107 CFU/只），每周4次，连续1个月。对照组仅给予载药（CMC）处理。细菌定植3周后，Pg+shATR组小鼠右腋窝接种KYSE150+ shNC细胞（1 × 106个细胞/只），对照组和Pg组小鼠右腋窝接种KYSE150+ shNC细胞（1 × 106个细胞/只）。每7天用卡尺测量肿瘤体积。实验4周后，腹腔注射d -荧光素钾盐150 mg/kg，戊巴比妥钠麻醉后进行生物发光成像。成像后处死小鼠，采用qRT-PCR、组织病理学检查和Western blot检测肿瘤组织中巨噬细胞极化情况。结果：在本研究中，我们发现Pg可以促进M0巨噬细胞向M2巨噬细胞极化，同时也促进KYSE150细胞的恶性进展。ATR被抑制后，ATR、磷酸化ATR （p-ATR）和M2巨噬细胞标志物（CD206、Arg1和VEGF）的表达水平下降。在BALB/c小鼠中，pg诱导的ATR激活通过募集m2型tam促进皮下植入肿瘤的生长。实验数据还表明M2极化、p-chik减小和ATR之间存在关联。结论：本研究发现Pg可激活共济失调-毛细血管扩张及rad3相关蛋白（ATR）信号通路，诱导M2肿瘤相关巨噬细胞（TAM）极化，从而促进ESCC的生长。这为ESCC的防治提供了新的理论依据。

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引用次数: 0

Predictors of Lesion Detection in a 12-Year Oral Cancer Screening Program: A Cohort Study in Northern Portugal 12年口腔癌筛查项目中病变检测的预测因素：葡萄牙北部的一项队列研究。

IF 2.3 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Oral Pathology & Medicine

Pub Date : 2026-09-01 Epub Date: 2026-06-23 DOI: 10.1111/jop.70162

Miguel Campos-Lopes, Fernanda Estevinho, Margarida Gouveia, Paulo Subtil, David Alfaiate, Paulo Nogueira, Paulo Sousa, João Leite-Moreira

Background

Oral cancer is a significant global public health issue, with high morbidity and mortality often linked to late diagnosis. This study evaluated a 12-year oral cancer screening program in northern Portugal to characterize its implementation and outcomes, identifying predictors of lesion detection. The program's outcomes were analyzed to assess its feasibility and contribution to early detection.

Methods

A retrospective analysis of screening program data from 2012 to 2024 was conducted. Participants were recruited through community outreach and selection of high-risk groups in primary care settings. A standardized oral/oropharyngeal examination protocol was used to classify lesions as benign, suspicious, or malignant based on clinical examination. Demographic data and risk factors were recorded, and individuals with suspicious or malignant lesions were referred for specialized evaluation.

Results

A total of 10 433 participants were screened (median age 63 years; 64% female). Oral lesions were detected in 16.7%, with 6.1% classified as suspicious and 0.2% malignant. Current smoking (OR = 1.65; p < 0.001), former smoking (OR = 1.27; p = 0.010), and previous oncological disease (OR = 1.74; p < 0.001) were associated with lesion detection on multivariable logistic regression. Overall, 16.4% of participants were referred for specialized consultation.

Conclusion

This large-scale screening program successfully reached a broad population, identifying a substantial number of potentially malignant lesions. The association with known risk factors supports the need for targeted screening strategies. Further research should integrate diagnostic confirmation, evaluate long-term patient outcomes, and assess cost-effectiveness to refine oral cancer screening policies and healthcare resource allocation.

背景：口腔癌是一个重要的全球公共卫生问题，其高发病率和死亡率往往与晚期诊断有关。本研究评估了葡萄牙北部一项为期12年的口腔癌筛查计划，以描述其实施和结果，确定病变检测的预测因素。该计划的结果进行了分析，以评估其可行性和对早期检测的贡献。方法：回顾性分析2012 ~ 2024年筛查项目资料。参与者是通过社区外展和选择初级保健机构的高危人群招募的。采用标准化的口腔/口咽检查方案，根据临床检查将病变分为良性、可疑或恶性。记录人口统计数据和危险因素，并将可疑或恶性病变的个体转诊进行专门评估。结果：共筛选了10433名参与者（中位年龄63岁，64%为女性）。口腔病变占16.7%，其中6.1%为可疑，0.2%为恶性。结论：这项大规模的筛查计划成功地覆盖了广泛的人群，发现了大量潜在的恶性病变。与已知危险因素的关联支持了有针对性筛查策略的必要性。进一步的研究应整合诊断确认，评估患者的长期预后，并评估成本效益，以完善口腔癌筛查政策和卫生保健资源分配。

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引用次数: 0

Prognostic Value of Bcl-xL in Head and Neck Squamous Cell Carcinoma: A Systematic Review and Meta-Analysis Bcl-xL在头颈部鳞状细胞癌中的预后价值：系统回顾和荟萃分析。

IF 2.3 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Oral Pathology & Medicine

Pub Date : 2026-09-01 Epub Date: 2026-06-13 DOI: 10.1111/jop.70155

L. Leinonen, R. Tiikkaja, M. Juola, P. Åström, T. Salo, K. Juurikka, K. Korelin

Background

Head and neck squamous cell carcinoma is a heterogeneous disease with poor survival outcomes. Bcl-xL regulates tumor cell survival and apoptosis and has been associated with metastasis and poor prognosis, although its overall role remains undeciphered.

Methods

We systematically evaluated the prognostic value of Bcl-xL in head and neck squamous cell carcinoma following PRISMA 2020 guidelines. We identified 2241 reports from seven databases, of which 20 original studies with overall good quality (REMARK) and low bias (QUIPS) were included.

Results

An association between Bcl-xL levels and survival was rarely observed. However, high Bcl-xL levels correlated with increased risk of lymph node metastasis in 40% of studies. A study on oral tongue cancer identified a significant correlation between high Bcl-xL levels and decreased survival and lymph node metastasis.

Conclusions

Current evidence does not support Bcl-xL as a general prognostic marker in head and neck squamous cell carcinoma. Nevertheless, Bcl-xL may have prognostic relevance in oral tongue cancer if further studies confirm the original finding.

背景：头颈部鳞状细胞癌是一种异质性疾病，生存预后差。Bcl-xL调节肿瘤细胞存活和凋亡，并与肿瘤转移和不良预后相关，但其整体作用尚不清楚。方法：我们按照PRISMA 2020指南系统评估Bcl-xL在头颈部鳞状细胞癌中的预后价值。我们从7个数据库中确定了2241份报告，其中包括20项总体质量良好（REMARK）和低偏倚（QUIPS）的原始研究。结果：很少观察到Bcl-xL水平与生存率之间的关联。然而，在40%的研究中，高Bcl-xL水平与淋巴结转移风险增加相关。一项针对口腔癌的研究发现，高Bcl-xL水平与生存率降低和淋巴结转移之间存在显著相关性。结论：目前的证据不支持Bcl-xL作为头颈部鳞状细胞癌的一般预后标志物。然而，如果进一步的研究证实了最初的发现，Bcl-xL可能与口腔癌的预后有关。

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引用次数: 0

Inhibition of the EP300/Notch Signaling Pathway Regulates Proliferation and Apoptosis in Oral Squamous Cell Carcinoma EP300/Notch信号通路抑制口腔鳞状细胞癌的增殖和凋亡

IF 2.3 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Oral Pathology & Medicine

Pub Date : 2026-09-01 Epub Date: 2026-06-16 DOI: 10.1111/jop.70159

Honglan Wang, Yanzhen Fu, Gaoren Lin, Dandan Zhang, Xiaoxu Nan, Jiaming Liu, Xuelin Mou, Changyue Liu, Yuxuan Fan, Tong Zheng, Ying Liu

Objective

Oral squamous cell carcinoma (OSCC) is highly recurrent and metastatic; EP300 drives tumorigenesis, but its mechanism is unclear.

Materials and Methods

EP300 expression was profiled in OSCC via databases, RT-PCR, and Western blot. Knockdown effects on proliferation (CCK-8, colony), cell cycle, and apoptosis (flow cytometry) were measured. EP300–Notch interplay was probed with Valproic acid, a Notch signaling activator (VPA) rescue assays.

Results

Our experimental results showed that EP300 was upregulated in OSCC Cell Lines. In addition, bioinformatics analysis showed that EP300 upregulation was significantly associated with poor prognosis of OSCC. Prior research and bioinformatics analyses have demonstrated a close relationship between EP300 and the activation of the notch signaling pathway in OSCC. After EP300 knockdown in OSCC cells treated with VPA, our results indicated that VPA could partially reverse the effects of EP300 knockdown on cell proliferation, cell cycle, apoptosis, and EMT processes in OSCC Cells.

Conclusion

In this study, we observed that EP300 knockdown suppressed the Notch signaling pathway, consequently inhibiting OSCC cell proliferation, the cell cycle, and EMT while also promoting apoptosis. These findings suggest that EP300 is crucial for OSCC cell growth and development.

目的：口腔鳞状细胞癌（OSCC）是一种高复发性和转移性的疾病；EP300驱动肿瘤发生，但其机制尚不清楚。材料和方法：通过数据库、RT-PCR和Western blot分析EP300在OSCC中的表达。检测敲低对增殖（CCK-8，菌落）、细胞周期和凋亡（流式细胞术）的影响。用丙戊酸（一种Notch信号激活剂）检测EP300-Notch的相互作用。结果：我们的实验结果显示EP300在OSCC细胞株中表达上调。此外，生物信息学分析显示，EP300上调与OSCC的不良预后显著相关。先前的研究和生物信息学分析表明，EP300与OSCC中notch信号通路的激活密切相关。在VPA处理的OSCC细胞中敲低EP300后，我们的研究结果表明，VPA可以部分逆转EP300敲低对OSCC细胞增殖、细胞周期、细胞凋亡和EMT过程的影响。结论：在本研究中，我们观察到EP300敲低抑制Notch信号通路，从而抑制OSCC细胞增殖、细胞周期和EMT，同时促进细胞凋亡。这些发现表明EP300对OSCC细胞的生长发育至关重要。

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引用次数: 0

Implications of ADP-Ribosylation Factor-Like Protein 4C (ARL4C) in Human Neoplasia With Special Emphasis on Ameloblastoma: A Scoping Review adp -核糖基化因子样蛋白4C （ARL4C）在人类肿瘤中的意义，特别强调成釉细胞瘤：范围综述。

IF 2.3 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Oral Pathology & Medicine

Pub Date : 2026-09-01 Epub Date: 2026-05-28 DOI: 10.1111/jop.70154

Muhammad Nazim Aiman Azhari, Chuey Chuan Tan, Lee Fah Yap, Siti Amalina Inche Zainal Abidin, Yet Ching Goh, Wanninayake Mudiyanselage Tilakaratne

Background

ADP-ribosylation factor-like protein 4C (ARL4C), a member of the ARF small GTP-binding protein subfamily, has emerged from its role as an epithelial growth regulator to become a central molecule in cancer biology. This scoping review was conducted according to the PRISMA-ScR framework, systematically maps literature from PubMed, Scopus and Web of Science up to December 2024 to synthesise evidence on ARL4C's regulatory mechanisms and its implications in tumourigenesis and relevance to ameloblastoma.

Findings

Originally recognised for its role in tissue morphogenesis, ARL4C is now known to drive cancer cell proliferation, migration and invasion when dysregulated. Its expression is modulated by major signalling cascades including Wnt/MAPK, PI3K/AKT and TGF-β, as well as epigenetic factors. While primarily acting as an oncogenic driver, ARL4C exhibits context-dependent roles, occasionally functioning as a tumour suppressor. In ameloblastoma, a locally aggressive odontogenic tumour, ARL4C expression correlates with invasion and bone resorption. Notably, this influence appears independent of BRAF mutation status, suggesting a unique pathological role in this setting.

Conclusion

ARL4C stands at the intersection of fundamental cell biology and clinical oncology. It holds promise as a biomarker for prognosis and recurrence and as a potential therapeutic target. However, its dual nature highlights the complexity of cancer signalling networks. Future research involving larger patient cohorts is essential to validate ARL4C's utility in precision oncology, particularly for the management of ameloblastoma.

背景：adp -核糖基化因子样蛋白4C （ARL4C）是ARF小gtp结合蛋白亚家族的一员，已经从上皮生长调节剂的角色发展成为癌症生物学的核心分子。本综述是根据PRISMA-ScR框架进行的，系统地绘制了截至2024年12月PubMed、Scopus和Web of Science的文献，以综合ARL4C的调控机制及其在肿瘤发生和成膜细胞瘤中的意义的证据。研究发现：ARL4C最初被认为在组织形态发生中起作用，现在已知当失调时，ARL4C可驱动癌细胞增殖、迁移和侵袭。其表达受Wnt/MAPK、PI3K/AKT、TGF-β等主要信号级联通路以及表观遗传因素的调控。虽然ARL4C主要作为致癌驱动因子，但它也表现出上下文依赖的作用，偶尔也会作为肿瘤抑制因子。在成釉细胞瘤（一种局部侵袭性牙源性肿瘤）中，ARL4C的表达与侵袭和骨吸收有关。值得注意的是，这种影响似乎独立于BRAF突变状态，表明在这种情况下具有独特的病理作用。结论：ARL4C处于基础细胞生物学和临床肿瘤学的交叉领域。它有望作为预后和复发的生物标志物以及潜在的治疗靶点。然而，它的双重性质突出了癌症信号网络的复杂性。为了验证ARL4C在精确肿瘤学中的应用，特别是在成釉细胞瘤的治疗中，涉及更大患者队列的未来研究是必不可少的。

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引用次数: 0

Oral Epithelial Dysplasia Information Needs Questionnaire (ODIN-Q): Responsiveness and Insights Into Patient Education 口腔上皮发育不良信息需求问卷（ODIN-Q）：对患者教育的反应性和洞察力。

IF 2.3 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Oral Pathology & Medicine

Pub Date : 2026-09-01 Epub Date: 2026-06-12 DOI: 10.1111/jop.70160

Waleed Alamoudi, Abdullah Alsoghier, Richeal Ni Riordain, Stefano Fedele, Stephen Porter

Background

The Oral Epithelial Dysplasia Informational Needs Questionnaire (ODIN-Q) was developed to assess the informational needs of patients with oral epithelial dysplasia (OED), a precancerous disorder associated with an increased risk of malignant transformation. This study aimed to evaluate the responsiveness of the ODIN-Q following an educational intervention using a written patient information leaflet about OED.

Methods

A prospective pre-post observational study was conducted at the Oral Medicine Unit at University College London Hospitals, between March 2023 and March 2025. Fifty patients with histologically confirmed OED completed the previously validated ODIN-Q before and after reading the leaflet. Differences between pre- and post-reading ODIN-Q scores were analysed using descriptive statistics and Cohen's d to determine effect sizes and responsiveness.

Results

Fifty participants (29 females, 21 males; mean age = 65 years) were included in the analysis. Overall ODIN-Q scores increased from 2.44 to 2.71, with a small overall effect size (d = 0.30; 95% CI: 0.01–0.59). The highest responsiveness was observed in the medical system and access to information domain (+0.42; +19%; d = 0.49; 95% CI: 0.19–0.79), followed by psychosocial aspects (+0.29; +12%; d = 0.38; 95% CI: 0.09–0.67) and physical aspects (+0.28; +11%; d = 0.36; 95% CI: 0.07–0.65). Other domains showed negligible to small responsiveness (investigative tests: d = 0.11; 95% CI: −0.18–0.40).

Conclusions

The ODIN-Q demonstrates preliminary evidence of responsiveness to changes in patients' informational needs following educational interventions, supporting its potential use as a multidomain measure for evaluating and guiding patient-centred education in OED.

背景：口腔上皮性发育不良信息需求问卷（ODIN-Q）的开发是为了评估口腔上皮性发育不良（OED）患者的信息需求，这是一种与恶性转化风险增加相关的癌前病变。本研究旨在评估教育干预后使用书面患者信息手册对OED的反应性。方法：2023年3月至2025年3月，在伦敦大学学院医院口腔医学部门进行了一项前瞻性前后观察性研究。50例组织学证实的OED患者在阅读小册子前后完成了先前验证的ODIN-Q。使用描述性统计和Cohen’s d来分析阅读前和阅读后ODIN-Q分数的差异，以确定效应大小和反应性。结果：50名参与者（女性29人，男性21人，平均年龄65岁）被纳入分析。总体ODIN-Q评分从2.44上升到2.71，总体效应值较小（d = 0.30; 95% CI: 0.01-0.59）。反应性最高的是医疗系统和信息获取领域（+0.42;+19%;d = 0.49; 95% CI: 0.19-0.79），其次是心理社会方面（+0.29;+12%;d = 0.38; 95% CI: 0.09-0.67）和身体方面（+0.28;+11%;d = 0.36; 95% CI: 0.07-0.65）。其他领域的反应性可以忽略不计（调查性测试：d = 0.11; 95% CI: -0.18-0.40）。结论：ODIN-Q初步证明了在教育干预后对患者信息需求变化的反应，支持其作为评估和指导以患者为中心的OED教育的多领域措施的潜在用途。

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引用次数: 0