在模拟失重条件下,大鼠视网膜核外层退化时,Müller 细胞被激活。

IF 5.9 2区 医学 Q2 CELL BIOLOGY Neural Regeneration Research Pub Date : 2025-07-01 Epub Date: 2024-03-01 DOI:10.4103/NRR.NRR-D-23-01035
Yuxue Mu, Ning Zhang, Dongyu Wei, Guoqing Yang, Lilingxuan Yao, Xinyue Xu, Yang Li, Junhui Xue, Zuoming Zhang, Tao Chen
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引用次数: 0

摘要

JOURNAL/nrgr/04.03/01300535-202507000-00032/figure1/v/2024-09-09T124005Z/r/image-tiff微重力环境已被证明会造成眼部损伤并影响视力,但其潜在机制仍不清楚。因此,我们通过尾悬挂建立了失重动物模型,以研究微重力环境下视网膜损伤的病理变化和分子机制。尾悬浮4周后,视网膜功能和形态没有明显改变,而尾悬浮8周后,视网膜功能明显下降,核外层变薄,凋亡细胞增多。为了探究视网膜核外层发生退行性变化的机制,研究人员利用蛋白质组学分析了尾悬8周后大鼠视网膜中不同表达的蛋白质。结果显示,与 Müller 细胞活化密切相关的成纤维细胞生长因子 2(又称碱性成纤维细胞生长因子)和胶质纤维酸性蛋白的表达水平显著上调。此外,在模拟失重 4 周和 8 周后,分别观察到了 Müller 细胞再生和 Müller 细胞胶质增生。这些研究结果表明,Müller细胞在失重期间视网膜核外层退化中发挥着重要的调节作用。
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Müller cells are activated in response to retinal outer nuclear layer degeneration in rats subjected to simulated weightlessness conditions.

JOURNAL/nrgr/04.03/01300535-202507000-00032/figure1/v/2024-09-09T124005Z/r/image-tiff A microgravity environment has been shown to cause ocular damage and affect visual acuity, but the underlying mechanisms remain unclear. Therefore, we established an animal model of weightlessness via tail suspension to examine the pathological changes and molecular mechanisms of retinal damage under microgravity. After 4 weeks of tail suspension, there were no notable alterations in retinal function and morphology, while after 8 weeks of tail suspension, significant reductions in retinal function were observed, and the outer nuclear layer was thinner, with abundant apoptotic cells. To investigate the mechanism underlying the degenerative changes that occurred in the outer nuclear layer of the retina, proteomics was used to analyze differentially expressed proteins in rat retinas after 8 weeks of tail suspension. The results showed that the expression levels of fibroblast growth factor 2 (also known as basic fibroblast growth factor) and glial fibrillary acidic protein, which are closely related to Müller cell activation, were significantly upregulated. In addition, Müller cell regeneration and Müller cell gliosis were observed after 4 and 8 weeks, respectively, of simulated weightlessness. These findings indicate that Müller cells play an important regulatory role in retinal outer nuclear layer degeneration during weightlessness.

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来源期刊
Neural Regeneration Research
Neural Regeneration Research CELL BIOLOGY-NEUROSCIENCES
CiteScore
8.00
自引率
9.80%
发文量
515
审稿时长
1.0 months
期刊介绍: Neural Regeneration Research (NRR) is the Open Access journal specializing in neural regeneration and indexed by SCI-E and PubMed. The journal is committed to publishing articles on basic pathobiology of injury, repair and protection to the nervous system, while considering preclinical and clinical trials targeted at improving traumatically injuried patients and patients with neurodegenerative diseases.
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