TaWRKY24 整合色氨酸代谢途径,参与防御小麦冠腐镰刀菌。

IF 6.2 1区 生物学 Q1 PLANT SCIENCES The Plant Journal Pub Date : 2024-11-05 DOI:10.1111/tpj.17079
Xing Xu, Tai-Fei Yu, Ji-Tong Wei, Xiao-Fei Ma, Yong-Wei Liu, Jin-Peng Zhang, Lei Zheng, Ze-Hao Hou, Jun Chen, Yong-Bin Zhou, Ming Chen, Jian Ma, Yun-Feng Jiang, Hu-Tai Ji, Li-Hui Li, You-Zhi Ma, Zhi-An Zhang, Zhao-Shi Xu
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引用次数: 0

摘要

小麦的生长过程一直面临着不利环境带来的严峻挑战。值得注意的是,由假根腐镰刀菌(Fp)引起的严重土传病害--冠腐镰刀菌(FCR)在各小麦种植区的发病率显著上升,导致粮食减产。然而,抗 FCR 的小麦品种的鉴定和有效基因资源的发掘尚未完成。在此,我们通过转录组和代谢组之间的相关性分析,筛选并确定了参与小麦抗FCR的色氨酸代谢途径,发现色氨酸代谢途径中的两种关键代谢产物吲哚-3-乙醛(IAAld)和褪黑激素在Fp诱导的小麦茎基部显著积累。有趣的是,外源施用这两种代谢物能显著增强小麦对 Fp 的抗性。此外,我们还观察到负责催化 IAAld 生成吲哚-3-乙酸(IAA)的关键酶 TaALDHase 的活性受到抑制。相反,在 Fp 诱导的小麦转录组中,参与褪黑激素生物合成的限速酶 TaMTase 的活性增强。进一步分析表明,TaWRKY24 可分别通过抑制 TaALDHase 的表达和增强 TaMTase 的转录来调控 IAA 和褪黑激素的生物合成。沉默 TaALDHase 可显著提高小麦对 FCR 的抗性。然而,干扰 TaWRKY24 或 TaMTase 则会降低小麦对 FCR 的抗性。总之,我们的研究结果证明了色氨酸代谢途径在赋予小麦抗FCR能力中的关键作用,从而扩大了其在植物系统中的生物功能范围。
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TaWRKY24 integrates the tryptophan metabolism pathways to participate in defense against Fusarium crown rot in wheat.

Wheat growth process has been experiencing severe challenges arising from the adverse environment. Notably, the incidence of Fusarium crown rot (FCR), a severe soil-borne disease caused by Fusarium pseudograminearum (Fp), has significantly intensified in various wheat-growing regions, resulting in a decline in grain yield. However, the identification of wheat varieties and the exploration of effective gene resources resistant to FCR have not yet been accomplished. Here, we screened and identified the tryptophan metabolism pathway to participate in wheat resistance to FCR by correlation analysis between transcriptome and metabolome, and found that indole-3-acetaldehyde (IAAld) and melatonin, two key metabolites in the tryptophan metabolic pathway, were significantly accumulated in Fp-induced wheat stem bases. Interestingly, exogenous application of these two metabolites could significantly enhance wheat resistance against Fp. Additionally, we observed that the activity of TaALDHase, a crucial enzyme responsible for catalyzing IAAld to produce indole-3-acetic acid (IAA), was inhibited. Conversely, the activity of TaMTase, a rate-limiting involved in melatonin biosynthesis, was enhanced in the Fp-induced wheat transcriptome. Further analysis showed that TaWRKY24 could regulate IAA and melatonin biosynthesis by inhibiting the expression of TaALDHase and enhancing the transcription of TaMTase, respectively. Silencing of TaALDHase could significantly increase wheat resistance to FCR. However, interference with TaWRKY24 or TaMTase could decrease wheat resistance to FCR. Collectively, our findings demonstrate the crucial role of the tryptophan metabolism pathway in conferring resistance against FCR in wheat, thereby expanding its repertoire of biological functions within the plant system.

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来源期刊
The Plant Journal
The Plant Journal 生物-植物科学
CiteScore
13.10
自引率
4.20%
发文量
415
审稿时长
2.3 months
期刊介绍: Publishing the best original research papers in all key areas of modern plant biology from the world"s leading laboratories, The Plant Journal provides a dynamic forum for this ever growing international research community. Plant science research is now at the forefront of research in the biological sciences, with breakthroughs in our understanding of fundamental processes in plants matching those in other organisms. The impact of molecular genetics and the availability of model and crop species can be seen in all aspects of plant biology. For publication in The Plant Journal the research must provide a highly significant new contribution to our understanding of plants and be of general interest to the plant science community.
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