Detection of Aminoglycosides Resistance among Strains of Escherichia Coli Isolated From Patients with Urinary Tract Infection

Abstract

Background: Urinary tract infections attack about 150 million people each year globally and nowadays there is a high emergence of antibiotic resistance strains among E. Coli species that cause UTI. The objective of this study was to detect Aminoglycosides resistance among E. Coli strains isolated from patients with urinary tract infection. Method: A total of 70 urine specimens were collected from patient with symptoms of urinary tract infection in sterile urine containers, and then inoculated in CLED media, incubated at 37c for 24 hours. E. Coli species were identified according to their colonial morphology, indirect gram staining reaction, and biochemical tests. Identified species were tested for antimicrobial susceptibility against the following aminoglycosides: Amikacin, Kanamycin, Streptomycin, Gentamicin, and Tobramycin using Kirby-bauer disc diffusion method. Results: The study revealed that 55.7% of urinary tract infections were caused by E. Coli. The study also showed that 12.8% of isolated E. Coli species were resistant to Amikacin, 28.2% to Gentamicin, 43.6% to Streptomycin, 48.7% to Kanamycin, and 53.8% to Tobramycin. Conclusion: The study concluded that the overall resistance of isolated E. coli species to aminoglycosides was 37.4% the most powerful Aminoglycoside against E. Coli species was Amikacin.