{"title":"Sub-Microfluidic Setup to Quantify Cell Surface & Charge Density","authors":"O. Borikov, O. M. Morozova, V. Berest","doi":"10.1109/UKRCON.2019.8879988","DOIUrl":null,"url":null,"abstract":"The purpose of this study is to create a prototype of an experimental setup for studying the electrical properties of biological cell surface by measuring electrophoretic mobility of cells and estimating zeta potential. We improved the microscopic method of observing cell electrophoresis in sub-microfluidic chamber with real-time video capture. The basic approaches to the analysis of received video images were figured out in order to correctly determine the linear and electrophoretic velocity of cells for further calculation of zeta potential. Automated processing of obtained experimental data allows faster statistical analysis of distribution of parameters within a cell population. Test measurements on erythrocytes and guidelines for the operation of the device have been developed.","PeriodicalId":211302,"journal":{"name":"2019 IEEE 2nd Ukraine Conference on Electrical and Computer Engineering (UKRCON)","volume":"41 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2019-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"2019 IEEE 2nd Ukraine Conference on Electrical and Computer Engineering (UKRCON)","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1109/UKRCON.2019.8879988","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 1
Abstract
The purpose of this study is to create a prototype of an experimental setup for studying the electrical properties of biological cell surface by measuring electrophoretic mobility of cells and estimating zeta potential. We improved the microscopic method of observing cell electrophoresis in sub-microfluidic chamber with real-time video capture. The basic approaches to the analysis of received video images were figured out in order to correctly determine the linear and electrophoretic velocity of cells for further calculation of zeta potential. Automated processing of obtained experimental data allows faster statistical analysis of distribution of parameters within a cell population. Test measurements on erythrocytes and guidelines for the operation of the device have been developed.
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