Stereological assessment of resveratrol’s impact on folliculogenesis and sex hormone levels in busulfan-treated rats

IF 1.9 F&S science Pub Date : 2026-07-01 Epub Date: 2026-03-02 DOI:10.1016/j.xfss.2026.02.002

Negin Rafati Nia M.Sc., Morteza Golbashirzadeh Ph.D., Atousa Moradzadegan Ph.D.

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Abstract

Objective

To study the stereological effects of resveratrol on folliculogenesis and sex hormone concentrations in busulfan-treated rats.

Design

Experimental animal study.

Subjects

Twenty-four adult female Sprague–Dawley rats.

Exposure

Animals were randomly assigned to 4 groups: control, busulfan (10 mg/kg, single intraperitoneal dose), busulfan plus resveratrol (10 mg/kg), and busulfan plus resveratrol (20 mg/kg). Resveratrol was administered orally for 35 days.

Main Outcome Measures

Serum concentrations of luteinizing hormone, follicle-stimulating hormone, estradiol, and progesterone were measured. Ovarian stereological analysis assessed follicle counts and corpus luteum volume.

Results

Busulfan exposure caused severe ovarian damage, including reduced estradiol and progesterone levels, diminished corpus luteum volume, and increased gonadotropins and atretic follicles. High-dose resveratrol (20 mg/kg) partially improved estradiol and progesterone concentrations, increased healthy follicle counts, and enhanced corpus luteum volume, while reducing gonadotropins and atretic follicles (P<.05). These findings indicate attenuation but not complete restoration of busulfan-induced ovarian dysfunction.

Conclusion

Busulfan disrupts ovarian function and impairs folliculogenesis. Resveratrol partially mitigates these adverse effects, suggesting its potential as a protective agent against chemotherapy-associated reproductive toxicity.

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白藜芦醇对布苏芬治疗大鼠卵泡发生和性激素水平影响的体视学评价。

目的：研究白藜芦醇对布苏芬治疗大鼠卵泡发生及性激素浓度的体视作用。设计：实验动物研究。对象：24只成年雌性Sprague-Dawley大鼠。暴露：动物随机分为4组：对照组、丁磺凡（10 mg/kg，单次腹腔注射）、丁磺凡加白藜芦醇（10 mg/kg）和丁磺凡加白藜芦醇（20 mg/kg）。口服白藜芦醇35 d。主要观察指标：测定血清黄体生成素、卵泡刺激素、雌二醇和黄体酮浓度。卵巢体视学分析评估卵泡计数和黄体体积。结果：布硫凡暴露导致卵巢严重损伤，包括雌二醇和黄体酮水平降低，黄体体积减少，促性腺激素增加和卵泡闭锁。高剂量白藜芦醇（20 mg/kg）部分提高雌二醇和黄体酮浓度，增加健康卵泡计数，增加黄体体积，同时降低促性腺激素和闭锁卵泡（P < 0.05）。这些结果表明，布苏芬引起的卵巢功能障碍减弱，但没有完全恢复。结论：布苏凡破坏卵巢功能，影响卵泡发生。白藜芦醇部分减轻了这些不良反应，这表明它有可能作为一种保护剂来对抗化疗相关的生殖毒性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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