Detection of deoxyribonucleases I and II (dnases I and II) activities in reproductive organs of male rabbits

International Journal of Biochemistry Pub Date : 1994-08-01 Epub Date: 2003-02-05 DOI:10.1016/0020-711X(94)90074-4
H. Takeshita, T. Yasuda, D. Nadano, E. Tenjo, K. Sawazaki, R. Iida, K. Kishi
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引用次数: 28

Abstract

  • 1.

    1. Deoxyribonucleases (DNases) I and II activities in 13 different organs and body fluids from healthy male rabbits were measured using the single radial enzyme diffusion method.

  • 2.

    2. We now show that testis, epididymis, ampulla, seminal vesicle, vesicular gland, prostate, and semen have both of the DNases I and II activities, whereas spermatozoa do not.

  • 3.

    3. DNase I activities were highest in epididymis and seminal vesicle, whereas DNase II activities were highest in epididymis and prostate among the reproductive organs.

  • 4.

    4. The presence of these two enzyme activities outside the digestive system suggests that they have another biological function in addition to their digestive roles.

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雄性家兔生殖器官脱氧核糖核酸酶I和II (dna酶I和II)活性的检测
1.1. 采用单径向酶扩散法测定了健康雄性家兔13种不同器官和体液中脱氧核糖核酸酶(dnase) I和II的活性。我们现在表明,睾丸、附睾、壶腹、精囊、囊泡腺、前列腺和精液都具有dnase I和dnase II的活性,而精子则没有。在生殖器官中,DNase I活性以附睾和精囊最高,而DNase II活性以附睾和前列腺最高。这两种酶在消化系统外的活性表明,除了消化作用外,它们还有另一种生物学功能。
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