Occurrence of Methicillin-resistant Staphylococcus aureus (MRSA) From Dairy Cows in Kebbi, Nigeria

Q4 Veterinary Iranian Journal of Veterinary Medicine Pub Date : 2023-01-01 DOI:10.32598/ijvm.17.1.1005256

Mohammed Sani Gaddafi, Y. Yakubu, Abdulkadir Usman Usman Junaidu, Muhammad Bashir Bello, Asinamai Athliamai Bitrus, Aliyu Ibrahim Musawa, B. Garba, H. Lawal

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Abstract

Background: Methicillin-resistant Staphylococcus aureus (MRSA) is an important healthcare-associated pathogen that is also an emerging zoonosis. Objectives: A cross-sectional study was conducted to investigate the prevalence of MRSA in dairy cattle farms in Kebbi, North-Western Nigeria. Methods: A total of 200 milk samples, consisting of 50 samples each from lactating cows, were collected and used. The samples were analyzed using bacterial culture and isolation and polymerase chain reaction (PCR). Suspected MRSA isolates were identified via PCR detection of the mecA gene, and antimicrobial susceptibility profiles of the isolates were assessed using the Kirby-Bauer disk diffusion method. Results: Of the two hundred milk samples examined, the prevalence of MRSA was recorded at 18% (36/200) using phenotypic and genotypic characterization methods. The susceptibility to vancomycin was observed in all isolates, and they had a multiple antibiotic resistance (MAR) index of >0.4. Conclusion: This investigation showed the colonization of healthy dairy cows by multidrug-resistant MRSA.

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尼日利亚凯比奶牛耐甲氧西林金黄色葡萄球菌的发生

背景:耐甲氧西林金黄色葡萄球菌(MRSA)是一种重要的卫生保健相关病原体，也是一种新兴的人畜共患病。目的:进行了一项横断面研究，以调查在尼日利亚西北部Kebbi的奶牛养殖场的MRSA流行情况。方法:采集奶牛乳样200份，每组50份。采用细菌培养分离和聚合酶链反应(PCR)对样品进行分析。采用PCR检测mecA基因，鉴定疑似MRSA分离株，采用Kirby-Bauer纸片扩散法评价分离株的药敏谱。结果:在检测的200份牛奶样本中，使用表型和基因型表征方法记录的MRSA患病率为18%(36/200)。所有分离株均对万古霉素敏感，多重抗生素耐药指数(MAR)为bb0 0.4。结论:本调查显示耐多药MRSA在健康奶牛中定植。

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