Isolation of a Variant of the Felid Herpesvirus 1-Live Attenuated Vaccine Strain F2 from Unvaccinated Cats.

IF 1.8 4区医学 Q3 VIROLOGY Intervirology Pub Date : 2026-01-01 Epub Date: 2026-03-30 DOI:10.1159/000551791

Yasuharu Suga, Akihiko Saito, Yasunari Kitamura, Rikio Kirisawa

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Abstract

Introduction: Felid herpesvirus 1 (FeHV-1) is widespread worldwide and is sometimes associated with chronic diseases in cats; therefore, it is clinically important. Vaccination can be used to control FeHV-1 infection. We previously isolated a strain identical to the live attenuated vaccine (LAV) strain F2 from a feline eye with a dendritic ulcer, indicating that the F2 strain may be pathogenic. The origin of the F2 strain that we previously isolated is unknown, and we surmised that the F2 strain may exist in the field. To determine whether the LAV strain F2 exists in the field, we, therefore, performed a genomic analysis of FeHV-1 isolates from cats.

Methods: FeHV-1 isolates from cats were genotyped using single-nucleotide variants (SNVs) within two marker genes related to the F2 strain as indicators. Strains with markers were subjected to next-generation sequencing to determine their whole-genome sequence (WGS).

Results: Of the nine FeHV-1 isolates, none were found to have two SNVs related to the F2 strain, whereas two isolates were found to have one SNV related to the F2 strain. Because the cats from which these strains were isolated lived together, the isolates were considered to be of the same strain, and we determined the WGS of one strain (strain A-19-5). All coding sequences of strain A-19-5 were identical to those of one of the cloned strains of the Merial vaccine (likely a variant of the F2 strain within the Merial vaccine population). The cat from which the strain was isolated had never received vaccination.

Conclusion: The results suggest that a strain identical to a variant of the F2 strain, as found in the FeHV-1-LAV, may exist in the field.

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从未接种疫苗的猫身上分离出一种变种的猫疱疹病毒1-减毒活疫苗株F2。

猫疱疹病毒1型（FeHV-1）在世界范围内广泛传播，有时与猫的慢性疾病有关；因此在临床上具有重要意义。疫苗接种可用于控制FeHV-1感染。我们先前从患有树突状溃疡的猫眼中分离出一株与减毒活疫苗（LAV）菌株F2相同的菌株，表明F2菌株可能具有致病性。我们之前分离到的F2菌株来源不明，我们推测F2菌株可能存在于野外。因此，为了确定LAV菌株F2是否在野外存在，我们对来自猫的FeHV-1分离株进行了基因组分析。方法：采用与F2菌株相关的两个标记基因内的单核苷酸变异（snv）为指标，对猫源FeHV-1分离株进行基因分型。对有标记的菌株进行下一代测序以确定其全基因组序列（WGS）。结果：9株FeHV-1分离株中未发现2株与F2株相关的SNV， 2株分离株发现1株与F2株相关的SNV。由于分离出这些菌株的猫生活在一起，因此这些分离物被认为是同一菌株，我们确定了其中一株（菌株A-19-5）的WGS。菌株a -19-5的所有编码序列与梅里亚疫苗的一个克隆菌株（可能是梅里亚疫苗群体中F2菌株的变体）的编码序列相同。分离出该菌株的那只猫从未接种过疫苗。结论：该菌株可能与FeHV-1-LAV中发现的F2变异株相同。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Intervirology 医学-病毒学

CiteScore

5.40

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： ''Intervirology'' covers progress in both basic and clinical virus research, and aims to provide a forum for the various disciplines within virology. Issues publishing original papers alternate with thematic issues, focusing on clearly defined topics. This thematic concentration serves to make timely reviews, research reports and controversy easily accessible to both specialists in the field and those who want to keep track of the latest developments outside their own area of interest. In addition to original papers, regular issues publish short communications and letters to the editor to provide readers with a forum for the exchange of ideas and comments. The scope encompasses work on the molecular biology of human and animal viruses, including genome organization and regulation, and the structure and function of viral proteins. The pathogenesis, immunology, diagnosis, epidemiology, prophylaxis and therapy of viral diseases are considered.