Induced Oxidative Hemolysis of Erythrocytes As a Model for Estimating the Antioxidant Activity of Pharmaceutical Substances

IF 4.033 Q4 Biochemistry, Genetics and Molecular Biology Biophysics Pub Date : 2026-09-14 DOI:10.1134/S0006350926700259

E. M. Sokolova, N. A. Dubenskaia, B. L. Psikha, N. I. Neshev

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Abstract

The kinetics of oxidative hemolysis of a 0.2% mouse erythrocyte suspension under the action of radical-forming initiators, hydrogen peroxide (HP), 2,2'-azobis(2-amidinopropane) dihydrochloride (AAPH), tert-butyl hydroperoxide (t-BuOOH), and cumene hydroperoxide (Cumyl-OOH), was studied over a wide concentration range. Erythrocyte hemolysis occurred upon activation of lipid peroxidation (LPO) in erythrocyte membranes. Hemolytic activity was characterized by the hemolysis induction period (I₁₀), which was determined graphically by the time it took to reach 10% hemolysis. The hemolytic effect of AAPH, t‑BuOOH was concentration-dependent. No concentration dependence was observed for HP and Cumyl-OOH. The known antioxidants 3-(3',5'-di-tert-butyl-4'-hydroxyphenyl) potassium propionate (fenosan), 6-methyl-2-ethyl-3-hydroxypyridine hydrochloride (epigid), and 4-hydroxy-2,2,6,6-tetramethylpiperidin-1-oxyl (Tempol) caused a concentration-dependent increase in the hemolysis induction period for both AAPH- and t-BuOOH-dependent initiation. The developed model of induced oxidative hemolysis can be used for testing the new pharmacological substances to antioxidant activity.

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诱导红细胞氧化溶血作为评价药物抗氧化活性的模型

研究了自由基形成引发剂过氧化氢（HP）、2,2′-偶氮（2-氨基丙烷）二盐酸（AAPH）、过氧化叔丁基（t-BuOOH）和过氧化异丙烯（Cumyl-OOH）在较宽浓度范围内对0.2%小鼠红细胞悬液氧化溶血的动力学。红细胞溶血发生于红细胞膜脂质过氧化（LPO）活化。溶血活动的特征是溶血诱导期（I10），这是由溶血达到10%所需的时间来确定的。AAPH、t - BuOOH的溶血作用呈浓度依赖性。HP和Cumyl-OOH均无浓度依赖性。已知的抗氧化剂3-（3′，5′-二叔丁基-4′-羟基苯基）丙酸钾（fenosan）、6-甲基-2-乙基-3-羟基吡啶盐酸盐（epigid）和4-羟基-2,2,6,6-四甲基胡椒碱-1-氧（Tempol）在AAPH-和t- buoh -依赖起始的溶血诱导期均引起浓度依赖性增加。所建立的诱导氧化溶血模型可用于检测新药理物质的抗氧化活性。

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来源期刊

Biophysics Biochemistry, Genetics and Molecular Biology-Biophysics

CiteScore

1.20

自引率

0.00%

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期刊介绍： Biophysics is a multidisciplinary international peer reviewed journal that covers a wide scope of problems related to the main physical mechanisms of processes taking place at different organization levels in biosystems. It includes structure and dynamics of macromolecules, cells and tissues; the influence of environment; energy transformation and transfer; thermodynamics; biological motility; population dynamics and cell differentiation modeling; biomechanics and tissue rheology; nonlinear phenomena, mathematical and cybernetics modeling of complex systems; and computational biology. The journal publishes short communications devoted and review articles.