Interaction of Resveratrol with DNA in Cells and Nucleosome-Protein Complexes In Vitro

Abstract

Resveratrol is a natural polyphenol with antibacterial, antifungal, antiviral, and antitumor activity. The mechanisms of resveratrol action require detailed study. In the present work, the ability of resveratrol to penetrate into the nuclei of mammalian cells and bind to DNA there was proven. It was found that in vitro resveratrol changes the conformation of the linker regions of DNA in chromatosomes containing linker histone H1.5, in contrast to chromatosomes with histone H1.0. It was found that resveratrol reduces the ability of the FACT/Nhp6 protein complex to reorganize nucleosomes and blocks the restoration of their structure after dissociation of FACT/Nhp6. The data obtained suggest that resveratrol may influence processes within the cell nucleus by interacting with DNA and altering the interaction between nucleosomes and chromatin-remodeling proteins. Potentially, it may also affect H1.5-dependent gene regulation.