首页 > 最新文献

生命科学最新文献

英文 中文
IF:
Reversible Glc-conjugation/hydrolysis modulates the homeostasis of lunularic acid in Marchantia polymorpha growth.
IF 6.2 1区 生物学 Q1 PLANT SCIENCES Pub Date : 2024-12-03 DOI: 10.1111/tpj.17166
Ting-Ting Zhu, Yu-Liang Xu, He Ta, Jiao-Zhen Zhang, Dan-Dan Xu, Jie Fu, Yue Hao, Ni-Hong Du, Ai-Xia Cheng, Hong-Xiang Lou

The circadian clock efficiently coordinates growth regulators and plant growth in the temporal regulation of physiological processes. The involvement of the growth-regulator-clock in governing the spatio-temporal regulation of plant growth and development remains unexplained in the nonvascular liverworts. In this study, we aimed to assess the relationship between the putative liverwort growth regulator lunularic acid (LA) levels and the growth variation of Marchantia polymorpha according to the circadian clock. LA level exhibited a similar circadian rhythm as gemmalings' accelerated growth during the light phase, and UV-B stress experiments implied that the surge in LA levels at the start of the light phase could serve as a circadian rhythm-based prediction for preempting UV-B injury, with LA serving as a protective shield against UV-B irradiation. Notably, Glc-conjugation/hydrolysis, buffering the anabolism-catabolism of endogenous LA, was rhythmically regulated. Furthermore, the reversible conversion between LA and LA-4'-O-glucoside (mediated by glucosyltransferases MpUGT744A1 and β-glucosidases MpBGLU2/3) was characterized both in vitro and in vivo and evidenced to be relevant to diurnal variation in LA level. Interaction between MpUGT744A1 and MpBGLU2 ensures the efficient metabolic flux between LA and LA-GE. These findings shed light on the regulatory mechanisms that govern LA metabolism and the importance of understanding the temporal aspects of LA for Marchantia's adaptive response to environmental stressors such as UV-B radiation.

{"title":"Reversible Glc-conjugation/hydrolysis modulates the homeostasis of lunularic acid in Marchantia polymorpha growth.","authors":"Ting-Ting Zhu, Yu-Liang Xu, He Ta, Jiao-Zhen Zhang, Dan-Dan Xu, Jie Fu, Yue Hao, Ni-Hong Du, Ai-Xia Cheng, Hong-Xiang Lou","doi":"10.1111/tpj.17166","DOIUrl":"https://doi.org/10.1111/tpj.17166","url":null,"abstract":"<p><p>The circadian clock efficiently coordinates growth regulators and plant growth in the temporal regulation of physiological processes. The involvement of the growth-regulator-clock in governing the spatio-temporal regulation of plant growth and development remains unexplained in the nonvascular liverworts. In this study, we aimed to assess the relationship between the putative liverwort growth regulator lunularic acid (LA) levels and the growth variation of Marchantia polymorpha according to the circadian clock. LA level exhibited a similar circadian rhythm as gemmalings' accelerated growth during the light phase, and UV-B stress experiments implied that the surge in LA levels at the start of the light phase could serve as a circadian rhythm-based prediction for preempting UV-B injury, with LA serving as a protective shield against UV-B irradiation. Notably, Glc-conjugation/hydrolysis, buffering the anabolism-catabolism of endogenous LA, was rhythmically regulated. Furthermore, the reversible conversion between LA and LA-4'-O-glucoside (mediated by glucosyltransferases MpUGT744A1 and β-glucosidases MpBGLU2/3) was characterized both in vitro and in vivo and evidenced to be relevant to diurnal variation in LA level. Interaction between MpUGT744A1 and MpBGLU2 ensures the efficient metabolic flux between LA and LA-GE. These findings shed light on the regulatory mechanisms that govern LA metabolism and the importance of understanding the temporal aspects of LA for Marchantia's adaptive response to environmental stressors such as UV-B radiation.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":" ","pages":""},"PeriodicalIF":6.2,"publicationDate":"2024-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142765131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Inside Front Cover Image
IF 6 1区 生物学 Q1 PLANT SCIENCES Pub Date : 2024-12-03 DOI: 10.1111/pce.15327
Qing Li, Yue He, Jian Feng, Yutong He, Sheng Zhang

Inside Front Cover: The cover image is based on the article Pseudomonas fluorescens Inoculation Enhances Salix matsudana Growth by Modifying Phyllosphere Microbiomes, Surpassing Nitrogen Fertilization by Qing Li et al., https://doi.org/10.1111/pce.15162.

{"title":"Inside Front Cover Image","authors":"Qing Li,&nbsp;Yue He,&nbsp;Jian Feng,&nbsp;Yutong He,&nbsp;Sheng Zhang","doi":"10.1111/pce.15327","DOIUrl":"https://doi.org/10.1111/pce.15327","url":null,"abstract":"<p>Inside Front Cover: The cover image is based on the article <i>Pseudomonas fluorescens Inoculation Enhances Salix matsudana Growth by Modifying Phyllosphere Microbiomes, Surpassing Nitrogen Fertilization</i> by Qing Li et al., https://doi.org/10.1111/pce.15162.\u0000\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure></p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":"48 1","pages":"ii"},"PeriodicalIF":6.0,"publicationDate":"2024-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/pce.15327","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142762253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Establishing a comprehensive web-based analysis platform for Nicotiana benthamiana genome and transcriptome.
IF 6.2 1区 生物学 Q1 PLANT SCIENCES Pub Date : 2024-12-03 DOI: 10.1111/tpj.17178
Ken-Ichi Kurotani, Hideki Hirakawa, Kenta Shirasawa, Koya Tagiri, Moe Mori, Abedelaziz Ramadan, Yasunori Ichihashi, Takamasa Suzuki, Yasuhiro Tanizawa, Jiyuan An, Christopher Winefield, Peter M Waterhouse, Kenji Miura, Yasukazu Nakamura, Sachiko Isobe, Michitaka Notaguchi

Nicotiana benthamiana has long served as a crucial plant material extensively used in plant physiology research, particularly in the field of plant pathology, because of its high susceptibility to plant viruses. Additionally, it serves as a production platform to test vaccines and other valuable substances. Among its approximately 3.1 Gb genome, 57 583 genes have been annotated within a 61 Mb region. We created a comprehensive and easy-to-use platform to use transcriptomes for modern annotation. These tools allow to visualize gene expression profiles, draw molecular evolutionary phylogenetic trees of gene families, perform functional enrichment analyses, and facilitate output downloads. To demonstrate their utility, we analyzed the gene expression profiles of enzymes within the nicotine biosynthesis pathway, a secondary metabolic pathway characteristic of the Nicotiana genus. Using the developed tool, expression profiles of the nicotine biosynthesis pathway genes were generated. The expression patterns of eight gene groups in the pathway were strongly expressed in the roots and weakly expressed in leaves and flowers of N. benthamiana. The results were consistent with the established gene expression profiles in Nicotiana tabacum and provided insights into gene family composition and expression trends. The compilation of this database tool can facilitate genetic analysis of N. benthamiana in the future.

{"title":"Establishing a comprehensive web-based analysis platform for Nicotiana benthamiana genome and transcriptome.","authors":"Ken-Ichi Kurotani, Hideki Hirakawa, Kenta Shirasawa, Koya Tagiri, Moe Mori, Abedelaziz Ramadan, Yasunori Ichihashi, Takamasa Suzuki, Yasuhiro Tanizawa, Jiyuan An, Christopher Winefield, Peter M Waterhouse, Kenji Miura, Yasukazu Nakamura, Sachiko Isobe, Michitaka Notaguchi","doi":"10.1111/tpj.17178","DOIUrl":"https://doi.org/10.1111/tpj.17178","url":null,"abstract":"<p><p>Nicotiana benthamiana has long served as a crucial plant material extensively used in plant physiology research, particularly in the field of plant pathology, because of its high susceptibility to plant viruses. Additionally, it serves as a production platform to test vaccines and other valuable substances. Among its approximately 3.1 Gb genome, 57 583 genes have been annotated within a 61 Mb region. We created a comprehensive and easy-to-use platform to use transcriptomes for modern annotation. These tools allow to visualize gene expression profiles, draw molecular evolutionary phylogenetic trees of gene families, perform functional enrichment analyses, and facilitate output downloads. To demonstrate their utility, we analyzed the gene expression profiles of enzymes within the nicotine biosynthesis pathway, a secondary metabolic pathway characteristic of the Nicotiana genus. Using the developed tool, expression profiles of the nicotine biosynthesis pathway genes were generated. The expression patterns of eight gene groups in the pathway were strongly expressed in the roots and weakly expressed in leaves and flowers of N. benthamiana. The results were consistent with the established gene expression profiles in Nicotiana tabacum and provided insights into gene family composition and expression trends. The compilation of this database tool can facilitate genetic analysis of N. benthamiana in the future.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":" ","pages":""},"PeriodicalIF":6.2,"publicationDate":"2024-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142765128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Inside Back Cover Image
IF 6 1区 生物学 Q1 PLANT SCIENCES Pub Date : 2024-12-03 DOI: 10.1111/pce.15328
Sheng Wang, Chaoyue Zhao, Yun Su, Kangjian Cao, Chao Mou, Fu Xu

Inside Back Cover: The cover image is based on the article A Framework for Measuring Tree Rings Based on Panchromatic Images and Deep Learning by Sheng Wang et al., https://doi.org/10.1111/pce.15091.

{"title":"Inside Back Cover Image","authors":"Sheng Wang,&nbsp;Chaoyue Zhao,&nbsp;Yun Su,&nbsp;Kangjian Cao,&nbsp;Chao Mou,&nbsp;Fu Xu","doi":"10.1111/pce.15328","DOIUrl":"https://doi.org/10.1111/pce.15328","url":null,"abstract":"<p>Inside Back Cover: The cover image is based on the article <i>A Framework for Measuring Tree Rings Based on Panchromatic Images and Deep Learning</i> by Sheng Wang et al., https://doi.org/10.1111/pce.15091.\u0000\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure></p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":"48 1","pages":"iii"},"PeriodicalIF":6.0,"publicationDate":"2024-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/pce.15328","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142762254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Mechanism of action of the toxic proline mimic azetidine 2-carboxylic acid in plants.
IF 6.2 1区 生物学 Q1 PLANT SCIENCES Pub Date : 2024-12-03 DOI: 10.1111/tpj.17154
William Thives Santos, Varun Dwivedi, Ha Ngoc Duong, Madison Miederhoff, Kathryn Vanden Hoek, Ruthie Angelovici, Craig A Schenck

Plants have an amazing capacity to outcompete neighboring organisms for space and resources. Toxic metabolites are major players in these interactions, which can have a broad range of effectiveness by targeting conserved molecular mechanisms, such as protein biosynthesis. However, lack of knowledge about defensive metabolite pathways, their mechanisms of action, and resistance mechanisms limits our ability to manipulate these pathways for enhanced crop resilience. Nonproteogenic amino acids (NPAAs) are a structurally diverse class of metabolites with a variety of functions but are typically not incorporated during protein biosynthesis. Here, we investigate the mechanism of action of the NPAA azetidine-2-carboxylic acid (Aze), an analog of the amino acid proline (Pro). Using a combination of plate-based assays, metabolite feeding, metabolomics, and proteomics, we show that Aze inhibits the root growth of Arabidopsis and other plants. Aze-induced growth reduction was restored by supplementing L-, but not D-Pro, and nontargeted proteomics confirm that Aze is misincorporated for Pro during protein biosynthesis, specifically on cytosolically translated proteins. Gene expression analysis, free amino acid profiling, and proteomics show that the unfolded protein response is upregulated during Aze treatment implicating that Aze misincorporation results in accumulation of misfolded proteins triggering a global stress response. This study demonstrates the mechanism of action of Aze in plants and provides a foundation for understanding the biological functions of proteotoxic metabolites.

{"title":"Mechanism of action of the toxic proline mimic azetidine 2-carboxylic acid in plants.","authors":"William Thives Santos, Varun Dwivedi, Ha Ngoc Duong, Madison Miederhoff, Kathryn Vanden Hoek, Ruthie Angelovici, Craig A Schenck","doi":"10.1111/tpj.17154","DOIUrl":"https://doi.org/10.1111/tpj.17154","url":null,"abstract":"<p><p>Plants have an amazing capacity to outcompete neighboring organisms for space and resources. Toxic metabolites are major players in these interactions, which can have a broad range of effectiveness by targeting conserved molecular mechanisms, such as protein biosynthesis. However, lack of knowledge about defensive metabolite pathways, their mechanisms of action, and resistance mechanisms limits our ability to manipulate these pathways for enhanced crop resilience. Nonproteogenic amino acids (NPAAs) are a structurally diverse class of metabolites with a variety of functions but are typically not incorporated during protein biosynthesis. Here, we investigate the mechanism of action of the NPAA azetidine-2-carboxylic acid (Aze), an analog of the amino acid proline (Pro). Using a combination of plate-based assays, metabolite feeding, metabolomics, and proteomics, we show that Aze inhibits the root growth of Arabidopsis and other plants. Aze-induced growth reduction was restored by supplementing L-, but not D-Pro, and nontargeted proteomics confirm that Aze is misincorporated for Pro during protein biosynthesis, specifically on cytosolically translated proteins. Gene expression analysis, free amino acid profiling, and proteomics show that the unfolded protein response is upregulated during Aze treatment implicating that Aze misincorporation results in accumulation of misfolded proteins triggering a global stress response. This study demonstrates the mechanism of action of Aze in plants and provides a foundation for understanding the biological functions of proteotoxic metabolites.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":" ","pages":""},"PeriodicalIF":6.2,"publicationDate":"2024-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142765129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
WHIRLY1 regulates aliphatic glucosinolate biosynthesis in early seedling development of Arabidopsis.
IF 6.2 1区 生物学 Q1 PLANT SCIENCES Pub Date : 2024-12-03 DOI: 10.1111/tpj.17181
Linh Thuy Nguyen, Pinelopi Moutesidi, Jörg Ziegler, Anike Glasneck, Solmaz Khosravi, Steffen Abel, Götz Hensel, Karin Krupinska, Klaus Humbeck

WHIRLY1 belongs to a family of plant-specific transcription factors capable of binding DNA or RNA in all three plant cell compartments that contain genetic materials. In Arabidopsis thaliana, WHIRLY1 has been studied at the later stages of plant development, including flowering and leaf senescence, as well as in biotic and abiotic stress responses. In this study, WHIRLY1 knockout mutants of A. thaliana were prepared by CRISPR/Cas9-mediated genome editing to investigate the role of WHIRLY1 during early seedling development. The loss-of-function of WHIRLY1 in 5-day-old seedlings did not cause differences in the phenotype and the photosynthetic performance of the emerging cotyledons compared with the wild type. Nevertheless, comparative RNA sequencing analysis revealed that the knockout of WHIRLY1 affected the expression of a small but specific set of genes during this critical phase of development. About 110 genes were found to be significantly deregulated in the knockout mutant, wherein several genes involved in the early steps of aliphatic glucosinolate (GSL) biosynthesis were suppressed compared with wild-type plants. The downregulation of these genes in WHIRLY1 knockout lines led to decreased GSL contents in seedlings and in seeds. Since GSL catabolism mediated by myrosinases was not altered during seed-to-seedling transition, the results suggest that AtWHIRLY1 plays a major role in modulation of aliphatic GSL biosynthesis during early seedling development. In addition, phylogenetic analysis revealed a coincidence between the evolution of methionine-derived aliphatic GSLs and the addition of a new WHIRLY in core families of the plant order Brassicales.

{"title":"WHIRLY1 regulates aliphatic glucosinolate biosynthesis in early seedling development of Arabidopsis.","authors":"Linh Thuy Nguyen, Pinelopi Moutesidi, Jörg Ziegler, Anike Glasneck, Solmaz Khosravi, Steffen Abel, Götz Hensel, Karin Krupinska, Klaus Humbeck","doi":"10.1111/tpj.17181","DOIUrl":"https://doi.org/10.1111/tpj.17181","url":null,"abstract":"<p><p>WHIRLY1 belongs to a family of plant-specific transcription factors capable of binding DNA or RNA in all three plant cell compartments that contain genetic materials. In Arabidopsis thaliana, WHIRLY1 has been studied at the later stages of plant development, including flowering and leaf senescence, as well as in biotic and abiotic stress responses. In this study, WHIRLY1 knockout mutants of A. thaliana were prepared by CRISPR/Cas9-mediated genome editing to investigate the role of WHIRLY1 during early seedling development. The loss-of-function of WHIRLY1 in 5-day-old seedlings did not cause differences in the phenotype and the photosynthetic performance of the emerging cotyledons compared with the wild type. Nevertheless, comparative RNA sequencing analysis revealed that the knockout of WHIRLY1 affected the expression of a small but specific set of genes during this critical phase of development. About 110 genes were found to be significantly deregulated in the knockout mutant, wherein several genes involved in the early steps of aliphatic glucosinolate (GSL) biosynthesis were suppressed compared with wild-type plants. The downregulation of these genes in WHIRLY1 knockout lines led to decreased GSL contents in seedlings and in seeds. Since GSL catabolism mediated by myrosinases was not altered during seed-to-seedling transition, the results suggest that AtWHIRLY1 plays a major role in modulation of aliphatic GSL biosynthesis during early seedling development. In addition, phylogenetic analysis revealed a coincidence between the evolution of methionine-derived aliphatic GSLs and the addition of a new WHIRLY in core families of the plant order Brassicales.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":" ","pages":""},"PeriodicalIF":6.2,"publicationDate":"2024-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142765136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The Turnip Yellows Virus Capsid Protein Promotes Access of Its Main Aphid Vector Myzus persicae to Phloem Tissues.
IF 6 1区 生物学 Q1 PLANT SCIENCES Pub Date : 2024-12-02 DOI: 10.1111/pce.15303
Maxime Verdier, Sylvaine Boissinot, Raymonde Baltenweck, Lise Negrel, Véronique Brault, Véronique Ziegler-Graff, Philippe Hugueney, Daniele Scheidecker, Célia Krieger, Quentin Chesnais, Martin Drucker

Many plant viruses modify the phenotype of their hosts, which may influence the behaviour of their vectors and facilitate transmission. Among them is the turnip yellows virus (TuYV), which can modify the orientation, feeding, and performance of its main aphid vector, Myzus persicae. However, the virus factors driving these mechanisms have not been elucidated. In this study, we compared the feeding behaviour and fecundity of aphids on TuYV-infected and transgenic Arabidopsis thaliana expressing individual TuYV proteins (CP, RT and P0) to define the role of these proteins in aphid-plant interactions. Aphids on TuYV-infected plants had shorter pathway phases and ingested phloem sap for longer times, which is expected to promote the acquisition of the phloem-limited TuYV. No change in aphid fecundity was observed on TuYV-infected plants. The transmission-conducive feeding behaviour changes could be fully reproduced by phloem-specific expression of the capsid protein (CP) in transgenic plants, whereas expression of P0 had minor and RT had no effects on aphid feeding behaviour. We then carried out a metabolomic analysis to determine plant compounds that could be involved in the modification of the aphid behaviour. A few metabolites were specific for TuYV-infected or CP-transgenic A. thaliana, and are good candidates for inducing behavioural changes.

{"title":"The Turnip Yellows Virus Capsid Protein Promotes Access of Its Main Aphid Vector Myzus persicae to Phloem Tissues.","authors":"Maxime Verdier, Sylvaine Boissinot, Raymonde Baltenweck, Lise Negrel, Véronique Brault, Véronique Ziegler-Graff, Philippe Hugueney, Daniele Scheidecker, Célia Krieger, Quentin Chesnais, Martin Drucker","doi":"10.1111/pce.15303","DOIUrl":"https://doi.org/10.1111/pce.15303","url":null,"abstract":"<p><p>Many plant viruses modify the phenotype of their hosts, which may influence the behaviour of their vectors and facilitate transmission. Among them is the turnip yellows virus (TuYV), which can modify the orientation, feeding, and performance of its main aphid vector, Myzus persicae. However, the virus factors driving these mechanisms have not been elucidated. In this study, we compared the feeding behaviour and fecundity of aphids on TuYV-infected and transgenic Arabidopsis thaliana expressing individual TuYV proteins (CP, RT and P0) to define the role of these proteins in aphid-plant interactions. Aphids on TuYV-infected plants had shorter pathway phases and ingested phloem sap for longer times, which is expected to promote the acquisition of the phloem-limited TuYV. No change in aphid fecundity was observed on TuYV-infected plants. The transmission-conducive feeding behaviour changes could be fully reproduced by phloem-specific expression of the capsid protein (CP) in transgenic plants, whereas expression of P0 had minor and RT had no effects on aphid feeding behaviour. We then carried out a metabolomic analysis to determine plant compounds that could be involved in the modification of the aphid behaviour. A few metabolites were specific for TuYV-infected or CP-transgenic A. thaliana, and are good candidates for inducing behavioural changes.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.0,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142765116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Hydrogen Sulphide: A Key Player in Plant Development and Stress Resilience.
IF 6 1区 生物学 Q1 PLANT SCIENCES Pub Date : 2024-12-02 DOI: 10.1111/pce.15309
Saumya Jaiswal, Surendra Pratap Singh, Samiksha Singh, Ravi Gupta, Durgesh Kumar Tripathi, Francisco J Corpas, Vijay Pratap Singh

Based on the research conducted so far, hydrogen sulphide (H2S) plays a crucial role in the development and stress resilience of plants. H2S, which acts as a signalling molecule, responds to different stresses such as heavy metals, drought, and salinity, and it regulates various aspects of plant growth and development including seed germination, root development, stomatal movement, flowering, and fruit ripening. Additionally, H2S is involved in mediating legume-Rhizobium symbiosis signalling. It modulates plant responses to external environmental stimuli by interacting with other signalling molecules like phytohormones, nitric oxide, and reactive oxygen species. Furthermore, H2S exerts these regulations since it can modify protein functions through a reversible thiol-based oxidative posttranslational modification called persulfidation, particularly in stress response and developmental processes. As a result, H2S is recognised as an important emerging signalling molecule with multiple roles in plants. Research in this field holds promise for engineering stress tolerance in crops and may lead to potential biotechnological applications in agriculture and environmental management.

{"title":"Hydrogen Sulphide: A Key Player in Plant Development and Stress Resilience.","authors":"Saumya Jaiswal, Surendra Pratap Singh, Samiksha Singh, Ravi Gupta, Durgesh Kumar Tripathi, Francisco J Corpas, Vijay Pratap Singh","doi":"10.1111/pce.15309","DOIUrl":"https://doi.org/10.1111/pce.15309","url":null,"abstract":"<p><p>Based on the research conducted so far, hydrogen sulphide (H<sub>2</sub>S) plays a crucial role in the development and stress resilience of plants. H<sub>2</sub>S, which acts as a signalling molecule, responds to different stresses such as heavy metals, drought, and salinity, and it regulates various aspects of plant growth and development including seed germination, root development, stomatal movement, flowering, and fruit ripening. Additionally, H<sub>2</sub>S is involved in mediating legume-Rhizobium symbiosis signalling. It modulates plant responses to external environmental stimuli by interacting with other signalling molecules like phytohormones, nitric oxide, and reactive oxygen species. Furthermore, H<sub>2</sub>S exerts these regulations since it can modify protein functions through a reversible thiol-based oxidative posttranslational modification called persulfidation, particularly in stress response and developmental processes. As a result, H<sub>2</sub>S is recognised as an important emerging signalling molecule with multiple roles in plants. Research in this field holds promise for engineering stress tolerance in crops and may lead to potential biotechnological applications in agriculture and environmental management.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.0,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142765105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Repressor MrERF4 and Activator MrERF34 Synergistically Regulate High Flavonol Accumulation Under UV-B Irradiation in Morella rubra Leaves.
IF 6 1区 生物学 Q1 PLANT SCIENCES Pub Date : 2024-12-02 DOI: 10.1111/pce.15310
Jiajia Li, Yunlin Cao, Yuan Meng, Tong Zhang, Jiafei Qian, Yilong Liu, Changqing Zhu, Bo Zhang, Kunsong Chen, Changjie Xu, Xian Li

Flavonols are important plant photoprotectants to defence UV-B irradiation, however, the underlying transcriptional regulatory mechanism of rapid flavonol accumulation in response to UV-B remains unknown. In this study, content of flavonols was significantly induced from 0.11 to 3.80 mg/g fresh weight by UV-B irradiation in leaves of Morella rubra seedlings. MrERF34 was identified as an activator that can regulate the expression of MrFLS2, and promoted flavonol biosynthesis with activator MrMYB12 under UV-B treatment. Transient overexpression of MrERF34 resulted in higher flavonol accumulation, while virus-induced gene silencing of MrERF34 reduced the content of flavonols in bayberry leaves. We further demonstrated that a repressor MrERF4 inhibited the expression of MrERF34 and MrMYB12 as well as MrFLS2 via ERF-associated-amphiphilic repression motif. Exposure to UV-B reduced the promoter activity and transcription of MrERF4, which weakened the inhibitory effect of MrERF4 on MrERF34, MrMYB12, and MrFLS2, leading to a tremendous accumulation of flavonols. Such inhibitory roles of MrERF4 in regulation of flavonol biosynthesis were further validated by transient overexpression in leaves of Nicotiana benthamiana and M. rubra. These findings enrich the synergistical regulatory mechanisms between repressor and activators in flavonol biosynthesis, and provide new insights into photoprotectants biosynthesis to mitigate UV-B stress in plants.

{"title":"Repressor MrERF4 and Activator MrERF34 Synergistically Regulate High Flavonol Accumulation Under UV-B Irradiation in Morella rubra Leaves.","authors":"Jiajia Li, Yunlin Cao, Yuan Meng, Tong Zhang, Jiafei Qian, Yilong Liu, Changqing Zhu, Bo Zhang, Kunsong Chen, Changjie Xu, Xian Li","doi":"10.1111/pce.15310","DOIUrl":"https://doi.org/10.1111/pce.15310","url":null,"abstract":"<p><p>Flavonols are important plant photoprotectants to defence UV-B irradiation, however, the underlying transcriptional regulatory mechanism of rapid flavonol accumulation in response to UV-B remains unknown. In this study, content of flavonols was significantly induced from 0.11 to 3.80 mg/g fresh weight by UV-B irradiation in leaves of Morella rubra seedlings. MrERF34 was identified as an activator that can regulate the expression of MrFLS2, and promoted flavonol biosynthesis with activator MrMYB12 under UV-B treatment. Transient overexpression of MrERF34 resulted in higher flavonol accumulation, while virus-induced gene silencing of MrERF34 reduced the content of flavonols in bayberry leaves. We further demonstrated that a repressor MrERF4 inhibited the expression of MrERF34 and MrMYB12 as well as MrFLS2 via ERF-associated-amphiphilic repression motif. Exposure to UV-B reduced the promoter activity and transcription of MrERF4, which weakened the inhibitory effect of MrERF4 on MrERF34, MrMYB12, and MrFLS2, leading to a tremendous accumulation of flavonols. Such inhibitory roles of MrERF4 in regulation of flavonol biosynthesis were further validated by transient overexpression in leaves of Nicotiana benthamiana and M. rubra. These findings enrich the synergistical regulatory mechanisms between repressor and activators in flavonol biosynthesis, and provide new insights into photoprotectants biosynthesis to mitigate UV-B stress in plants.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.0,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142765108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
CsCBF1/CsZHD9-CsMADS27, a critical gene module controlling dormancy and bud break in tea plants.
IF 6.2 1区 生物学 Q1 PLANT SCIENCES Pub Date : 2024-12-02 DOI: 10.1111/tpj.17165
Xinyuan Hao, Junwei Tang, Yao Chen, Chao Huang, Weifu Zhang, Ying Liu, Chuan Yue, Lu Wang, Changqing Ding, Wenhao Dai, Yajun Yang, David P Horvath, Xinchao Wang

Tea plants are perennial evergreen woody crops that originated in low latitudes but have spread to high latitudes. Bud dormancy is an important adaptation mechanism to low temperatures, and its timing is economically significant for tea production. However, the core molecular networks regulating dormancy and bud break in tea plants remain unclear. In the present study, a MADS-box transcription factor CsMADS27 was identified in tea plants. Gene and phenotype characterizations following ectopic overexpression and endogenous silencing experiments are consistent with a role for CsMADS27 in dormancy and sprouting in different tea cultivars. Furthermore, CsDJC23 was found to be a downstream target of CsMADS27 and implicated in bud sprouting. Based on yeast one-hybrid screening and comprehensive verification, CsCBF1 and CsZHD9 were identified as upstream transcriptional inhibitors and activators of CsMADS27, respectively, with the two proteins showing direct interactions and competitive binding effects. Histone acetylation (H3K27Ac) in the first exon and intron regions of CsMADS27 was associated with a positive role in CsMADS27 expression. These results revealed that CsMADS27 is a key transcription factor involved in the regulation of dormancy and bud break. Furthermore, the CsCBF1/CsZHD9-CsMADS27 module plays a critical role in sensing environmental factors and accurately regulating the growth and development of overwintering buds in tea plants.

{"title":"CsCBF1/CsZHD9-CsMADS27, a critical gene module controlling dormancy and bud break in tea plants.","authors":"Xinyuan Hao, Junwei Tang, Yao Chen, Chao Huang, Weifu Zhang, Ying Liu, Chuan Yue, Lu Wang, Changqing Ding, Wenhao Dai, Yajun Yang, David P Horvath, Xinchao Wang","doi":"10.1111/tpj.17165","DOIUrl":"https://doi.org/10.1111/tpj.17165","url":null,"abstract":"<p><p>Tea plants are perennial evergreen woody crops that originated in low latitudes but have spread to high latitudes. Bud dormancy is an important adaptation mechanism to low temperatures, and its timing is economically significant for tea production. However, the core molecular networks regulating dormancy and bud break in tea plants remain unclear. In the present study, a MADS-box transcription factor CsMADS27 was identified in tea plants. Gene and phenotype characterizations following ectopic overexpression and endogenous silencing experiments are consistent with a role for CsMADS27 in dormancy and sprouting in different tea cultivars. Furthermore, CsDJC23 was found to be a downstream target of CsMADS27 and implicated in bud sprouting. Based on yeast one-hybrid screening and comprehensive verification, CsCBF1 and CsZHD9 were identified as upstream transcriptional inhibitors and activators of CsMADS27, respectively, with the two proteins showing direct interactions and competitive binding effects. Histone acetylation (H3K27Ac) in the first exon and intron regions of CsMADS27 was associated with a positive role in CsMADS27 expression. These results revealed that CsMADS27 is a key transcription factor involved in the regulation of dormancy and bud break. Furthermore, the CsCBF1/CsZHD9-CsMADS27 module plays a critical role in sensing environmental factors and accurately regulating the growth and development of overwintering buds in tea plants.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":" ","pages":""},"PeriodicalIF":6.2,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142765127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1