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Study on the photo/electrochemical bi-functional properties of a coupling interface of Ru[dcbpy]32+-AMT/Au by SECM imaging-based joint analytical method. 利用基于 SECM 成像的联合分析方法研究 Ru[dcbpy]32+-AMT/Au 耦合界面的光/电化学双功能特性。
IF 5.6 1区 化学 Q1 CHEMISTRY, ANALYTICAL Pub Date : 2024-09-01 Epub Date: 2024-06-18 DOI: 10.1016/j.talanta.2024.126423
Zhigang Yu, Yangkejia Xiang, Xianda Han, Yi Guang, Fengqin Li

A photo/electrochemical coupling interface of Ru[dcbpy]32+-AMT/Au (AMT; 5-Amino-1,3,4-thiadiazole-2-thiol) was fabricated using a dehydration condensation sulfhydrating method. For the interface functional properties, a combined dual-signal recording (CDSR) method was applied to characterize the response characteristics, and a scanning electrochemical microscopy-electrochemiluminescence (SECM-ECL) imaging was developed to assess the interface distribution uniformity. The interface biosensing compatibility was validated by constructing a simple DNA sensor. The research results show that the interaction between the two functional parameters follows a synergistic effect mechanism in the coupling conditions and an interference effect mechanism in the detection condition. Under optimized conditions, the saturation dual-signal response values are 156.0 and 86.8 μA, respectively. The statistics and imaging comparison analysis validate good interface distribution uniformity and stability performance. The DNA sensor's dual-signal detection limits to the signal probe (SP) are ∼30 fM and 0.3 pM with linear ranges of 100.0 fM ∼ 1.0 nM and 1.0 pM ∼ 10.0 nM, respectively. The fabricated interface exhibits an effective bi-functional response performance compatible with biosensing. The proposed imaging method has a high technical fit for studying photo/electrochemical coupling interfaces and can also provide a reference for other similar coupling interface analyses.

利用脱水缩合硫水法制备了 Ru[dcbpy]32+-AMT/Au (AMT;5-氨基-1,3,4-噻二唑-2-硫醇)的光/电化学耦合界面。在界面功能特性方面,采用了双信号联合记录(CDSR)方法来表征响应特性,并开发了扫描电化学显微镜-电化学发光(SECM-ECL)成像技术来评估界面分布的均匀性。通过构建一个简单的 DNA 传感器验证了界面生物传感的兼容性。研究结果表明,两个功能参数之间的相互作用在耦合条件下遵循协同效应机制,而在检测条件下则遵循干扰效应机制。在优化条件下,饱和双信号响应值分别为 156.0 和 86.8 μA。统计和成像对比分析验证了良好的界面分布均匀性和稳定性能。DNA 传感器对信号探针(SP)的双信号检测限分别为 30 fM 和 0.3 pM,线性范围分别为 100.0 fM ∼ 1.0 nM 和 1.0 pM ∼ 10.0 nM。制备的界面具有与生物传感兼容的有效双功能响应性能。所提出的成像方法在研究光/电化学耦合界面方面具有很高的技术契合度,也可为其他类似的耦合界面分析提供参考。
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引用次数: 0
Aptamer molecular gate functionalized mesoporous SiO2@MB controlled-release system for pollutant detection using Ti(Ⅲ) self-doped TiO2 NTs as active photoanode coupled with electrostatic modulation. 以 Ti(Ⅲ)自掺杂 TiO2 NTs 为活性光阳极并结合静电调制的 Aptamer 分子门功能化介孔 SiO2@MB 控制释放系统用于污染物检测。
IF 5.6 1区 化学 Q1 CHEMISTRY, ANALYTICAL Pub Date : 2024-09-01 Epub Date: 2024-06-18 DOI: 10.1016/j.talanta.2024.126409
Ziwei Zhang, Yanru Wang, Qichen Chen, Xiaojiang Tan, Xialin Hu, Yarui An, Meichuan Liu

Atrazine (ATZ) is a widely used herbicide that can cause serious harm to organisms and ecosystems. An immobilization-free photoelectrochemical (PEC) aptasensor has been herein developed for ATZ based on aptamer molecular gate functionalized mesoporous SiO2@MB controlled release system. Compared with traditional immobilization-based sensors, immobilization-free sensors (IFSs) avoid the modification of the recognition element on the electrode surface. Mesoporous SiO2 with large surface area and good biocompatibility can be used as nanocontainers to stably encapsulate the signal shuttle molecule methylene blue (MB). The bifunctional aptamer (APT) is used not only as the recognition element for ATZ but also as the signal switch to block or release MB. In the presence of ATZ, the specific recognition between ATZ and APT will cause the detachment of APT from the surface of SiO2, thus the molecular gate will open and release MB. Due to pH modulation, the positively charged MB can reach the surface of the negatively charged Ti(III) self-doped TiO2 NTs (Ti(III)-TiO2 NTs) electrode to act as an electron donor, which increases the photocurrent. The immobilization-free aptasensor has shown ultrasensitive detection of ATZ with a wide linear range from 1.0 pM to 100.0 nM and a low detection limit of 0.1 pM. In addition, the sensor has excellent selectivity, stability and anti-interference ability, and has been used in real water sample analysis successfully. This strategy has provided a new idea for the design of advanced immobilization-free PEC sensors for environmental pollutant detection.

阿特拉津(ATZ)是一种广泛使用的除草剂,可对生物和生态系统造成严重危害。本文开发了一种无固定化光电化学(PEC)适配子传感器,该传感器基于适配子分子门功能化介孔二氧化硅@MB控释系统,用于检测阿特拉津。与传统的固定化传感器相比,无固定化传感器(IFS)避免了对电极表面识别元件的修饰。介孔二氧化硅具有较大的比表面积和良好的生物相容性,可用作纳米容器稳定地包封信号穿梭分子亚甲基蓝(MB)。双功能适配体(APT)不仅可用作 ATZ 的识别元件,还可用作阻断或释放 MB 的信号开关。在 ATZ 存在的情况下,ATZ 与 APT 之间的特异性识别会导致 APT 从 SiO2 表面脱离,从而打开分子门释放 MB。由于 pH 值的调节,带正电荷的 MB 可以到达带负电荷的 Ti(III)自掺杂 TiO2 NTs(Ti(III)-TiO2 NTs)电极表面,充当电子供体,从而增加光电流。这种无固定化诱导传感器对 ATZ 的检测具有超灵敏度,线性范围从 1.0 pM 到 100.0 nM,检测限低至 0.1 pM。此外,该传感器还具有优异的选择性、稳定性和抗干扰能力,并已成功应用于实际水样分析。这一策略为设计先进的无固定化 PEC 传感器检测环境污染物提供了新思路。
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引用次数: 0
Stable detection of diazinon residues in vegetables by an electrochemiluminescent aptasensor based on the in-situ production of H2O2 from dual-catalytic glucose. 基于双催化葡萄糖原位产生 H2O2 的电化学发光适体传感器稳定检测蔬菜中的二嗪农残留。
IF 5.6 1区 化学 Q1 CHEMISTRY, ANALYTICAL Pub Date : 2024-09-01 Epub Date: 2024-06-17 DOI: 10.1016/j.talanta.2024.126443
Jingcheng Huang, Mei Zhang, Xue Huang, He Li, Jie Han, Shancang Zhao, Mohamed Bedair Mohamed Ahmed, Xia Sun, Yemin Guo

Stable detection of diazinon (DZN) residues in vegetables is important for food safety. In this work, an electrochemiluminescence (ECL) aptasensor with dual-catalytic glucose in-situ production of H2O2 was constructed for the stable detection of DZN in vegetables. Firstly, MWCNTs@MB was prepared using π-π stacking interactions between methylene blue (MB) and multi-walled carbon nanotubes (MWCNTs) to enhance the loading of MB on an electrode and thus catalyze the generation of H2O2 from glucose. Secondly, Cu2O@AuNPs was formed by loading AuNPs on the surface of Cu2O through spontaneous reduction reaction, which improved the interfacial charge transfer, Cu2O nano-enzyme had glucose oxidase mimicking activity and could further catalyze the production of more H2O2 from glucose. MWCNTs@MB and Cu2O@AuNPs played a key role in the in-situ generation of co-reacting reagent H2O2, which solved the problem of unstable detection caused by the easy decomposition of the H2O2 solution added to the luminescence system. In addition, the aptamer was immobilized on the electrode surface by forming Au-S bonds with Cu2O@AuNPs. As a result, the ECL aptasensor performed good linearity in 1.00 pg mL-1-1.00 μg mL-1 and a low limit of detection (LOD) to 0.39 pg mL-1 (S/N = 3). This work provided an effective method for the accurate and stable detection of DZN residues in vegetables, which was of great significance in ensuring food safety and assessing the environmental risk of DZN.

稳定检测蔬菜中的二嗪农(DZN)残留对食品安全非常重要。本研究构建了一种具有葡萄糖原位产生 H2O2 双催化的电化学发光(ECL)诱导传感器,用于稳定检测蔬菜中的 DZN。首先,利用亚甲基蓝(MB)与多壁碳纳米管(MWCNTs)之间的π-π堆积相互作用制备了MWCNTs@MB,以提高MB在电极上的负载,从而催化葡萄糖产生H2O2。其次,通过自发还原反应在 Cu2O 表面负载 AuNPs 形成 Cu2O@AuNPs,从而改善了界面电荷转移,Cu2O 纳米酶具有葡萄糖氧化酶模拟活性,可进一步催化葡萄糖产生更多的 H2O2。MWCNTs@MB 和 Cu2O@AuNPs 在原位生成共反应试剂 H2O2 中发挥了关键作用,解决了加入发光系统的 H2O2 溶液易分解导致检测不稳定的问题。此外,通过与 Cu2O@AuNPs 形成 Au-S 键,将适配体固定在电极表面。结果,ECL 快速传感器在 1.00 pg mL-1-1.00 μg mL-1 范围内线性良好,检测限(LOD)低至 0.39 pg mL-1(信噪比为 3)。该研究为准确、稳定地检测蔬菜中DZN的残留提供了有效的方法,对保障食品安全和评估DZN的环境风险具有重要意义。
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引用次数: 0
Serum metabolomics reveals the effectiveness of human placental mesenchymal stem cell therapy for Crohn's disease. 血清代谢组学揭示了人胎盘间充质干细胞疗法对克罗恩病的疗效。
IF 5.6 1区 化学 Q1 CHEMISTRY, ANALYTICAL Pub Date : 2024-09-01 Epub Date: 2024-06-16 DOI: 10.1016/j.talanta.2024.126442
Xiao Wang, Dandan Shang, Junyao Chen, Sheng Cheng, Deying Chen, Zhehua Zhang, Chaoxu Liu, Jiong Yu, Hongcui Cao, Liang Li, Lanjuan Li

Mesenchymal stem cell (MSC) therapy offers a promising cure for Crohn's disease (CD), however, its therapeutic effects vary significantly due to individual differences. Therefore, identifying easily detectable biomarkers is essential to assess the efficacy of MSC therapy. In this study, SAMP1/Yit mice were used as a model of CD, which develop spontaneous chronic ileitis, closely resembling the characteristics present in CD patients. Serum metabolic alterations during treatment were analyzed, through the application of differential 12C-/13C-dansylation labeling liquid chromatography-mass spectrometry. Based on the significant differences and time-varying trends of serum amine/phenol-containing metabolites abundance between the control group, the model group, and the treatment group, four serum biomarkers were ultimately screened for evaluating the efficacy of MSC treatment for CD, namely 4-hydroxyphenylpyruvate, 4-hydroxyphenylacetaldehyde, caffeate, and N-acetyltryptamine, whose abundances both increased in the serum of CD model mice and decreased after MSC treatment. These metabolic alterations were associated with tyrosine metabolism, which was validated by the dysregulation of related enzymes. The discovery of biomarkers may help to improve the targeting and effectiveness of treatment and provide innovative prospects for the clinical application of MSC for CD.

间充质干细胞(MSC)疗法有望治愈克罗恩病(CD),但由于个体差异,其治疗效果存在很大差异。因此,确定易于检测的生物标志物对于评估间充质干细胞疗法的疗效至关重要。本研究使用 SAMP1/Yit 小鼠作为 CD 模型,这种小鼠会发生自发性慢性回肠炎,与 CD 患者的特征非常相似。通过应用差异12C-/13C-丹酰化标记液相色谱-质谱法分析了治疗期间血清代谢的变化。根据对照组、模型组和治疗组血清中含胺类/酚类代谢物丰度的显著差异和时变趋势,最终筛选出4种血清生物标志物,即4-羟基苯基丙酮酸盐、4-羟基苯基乙醛、咖啡酸盐和N-乙酰色胺,用于评估间充质干细胞治疗CD的疗效。这些代谢改变与酪氨酸代谢有关,相关酶的失调验证了这一点。生物标志物的发现可能有助于提高治疗的针对性和有效性,并为间充质干细胞治疗CD的临床应用提供创新前景。
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引用次数: 0
A facile and on-site sensing strategy for phenolic compounds based on a novel nanozyme with high polyphenol oxidase-like activity. 基于具有高多酚氧化酶样活性的新型纳米酶的酚类化合物简便现场传感策略。
IF 5.6 1区 化学 Q1 CHEMISTRY, ANALYTICAL Pub Date : 2024-09-01 Epub Date: 2024-06-18 DOI: 10.1016/j.talanta.2024.126422
Le Wang, Ling Ruan, Hao Zhang, Yue Sun, Wenqi Shi, Hui Huang, Yongxin Li

Phenolic compounds (PCs) are diverse in nature and undergo complex migration and transformations in the environment, making it challenging to use techniques such as chromatography and other traditional methods to determine the concentration of PCs by separation, individual monitoring and subsequent addition. To address this issue, a facile and on-site strategy was developed to measure the concentration of PCs using a novel nanozyme with polyphenol oxidase-like activity. First, the nanozyme was designed by coordinating the asymmetric ligand nicotinic acid with copper to mimic the structure of mononuclear and trinuclear copper clusters of natural laccases. Subsequently, by introducing 2-mercaptonicotinic acid to regulate the valence state of copper, the composite nanozyme CuNA10S was obtained with significantly enhanced activity. Interestingly, CuNA10S was shown to have a broad substrate spectrum capable of catalyzing common PCs. Building upon the superior performance of this nanozyme, a method was developed to determine the concentration of PCs. To enable rapid on-site sensing, we designed and prepared CuNA10S-based test strips and developed a tailored smartphone sensing platform. Using paper strip sensors combined with a smartphone sensing platform with RGB streamlined the sensing process, facilitating rapid on-site analysis of PCs within a range of 0-100 μM. Our method offers a solution for the quick screening of phenolic wastewater at contaminated sites, allowing sensitive and quick monitoring of PCs without the need for standard samples. This significantly simplifies the monitoring procedure compared to more cumbersome large-scale instrumental methods.

酚类化合物(PCs)的性质多种多样,在环境中会发生复杂的迁移和转化,因此使用色谱等技术和其他传统方法通过分离、单独监测和后续添加来确定 PCs 的浓度具有挑战性。为解决这一问题,我们开发了一种简便的现场策略,利用一种具有多酚氧化酶活性的新型纳米酶来测量多氯联苯的浓度。首先,通过将不对称配体烟酸与铜配位,模仿天然裂解酶的单核和三核铜簇结构,设计了这种纳米酶。随后,通过引入 2-巯基烟酸来调节铜的价态,得到了活性显著增强的复合纳米酶 CuNA10S。有趣的是,CuNA10S 具有广泛的底物谱,能够催化常见的 PCs。基于这种纳米酶的卓越性能,我们开发了一种测定多氯联苯浓度的方法。为了实现现场快速检测,我们设计并制备了基于 CuNA10S 的试纸,并开发了一个量身定制的智能手机检测平台。纸条传感器与带 RGB 的智能手机传感平台的结合使用简化了传感过程,有助于现场快速分析 0-100 μM 范围内的多氯联苯。我们的方法为快速筛查受污染场地的酚类废水提供了解决方案,无需标准样品即可灵敏、快速地监测多氯联苯。与更为繁琐的大型仪器方法相比,这大大简化了监测程序。
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引用次数: 0
Photonic crystal-enhanced fluorescence biosensor with logic gate operation based on one-pot cascade amplification DNA circuit for enzyme-free and ultrasensitive analysis of two microRNAs. 基于一次级联放大 DNA 电路的逻辑门操作的光子晶体增强荧光生物传感器,用于对两种 microRNA 进行免酶超灵敏分析。
IF 5.6 1区 化学 Q1 CHEMISTRY, ANALYTICAL Pub Date : 2024-09-01 Epub Date: 2024-06-19 DOI: 10.1016/j.talanta.2024.126428
Ziyi Tian, Jie Luo, Chuyan Zhang, Yongru Li, Shunming Hu, Yongxin Li

The development of sensitive and efficient analytical methods for multiple biomarkers is crucial for cancer screening at early stage. MicroRNAs (miRNAs) are a kind of biomarkers with diagnostic potential for cancer. However, the ultrasensitive and logical analysis of multiple miRNAs with simple operation still faces some challenges. Herein, a photonic crystal (PC)-enhanced fluorescence biosensor with logic gate operation based on one-pot cascade amplification DNA circuit was developed for enzyme-free and ultrasensitive analysis of two cancer-related miRNAs. The fluorescence biosensor was performed by biochemical recognition amplification module (BCRAM) and physical enhancement module (PEM) to achieve logical and sensitive detection. In the BCRAM, one-pot cascade amplification circuit consisted of the upstream parallel entropy-driven circuit (EDC) and the downstream shared catalytic hairpin assembly (CHA). The input of target miRNA would trigger each corresponding EDC, and the parallel EDCs released the same R strand for triggering subsequent CHA; thus, the OR logic gate was obtained with minimization of design and operation. In the PEM, photonic crystal (PC) array was prepared easily for specifically enhancing the fluorescence output from BCRAM by the optical modulation capabilities; meanwhile, the high-throughput signal readout was achieved by microplate analyzer. Benefiting from the integrated advantages of two modules, the proposed biosensor achieved ultrasensitive detection of two miRNAs with easy logic gate operation, obtaining the LODs of 8.6 fM and 6.7 fM under isothermal and enzyme-free conditions. Hence, the biosensor has the advantages of high sensitivity, easy operation, multiplex and high-throughput analysis, showing great potential for cancer screening at early stage.

开发灵敏高效的多种生物标志物分析方法对于早期癌症筛查至关重要。微小核糖核酸(miRNA)是一种具有癌症诊断潜力的生物标志物。然而,如何通过简单的操作对多种 miRNAs 进行超灵敏、合理的分析仍面临一些挑战。本文开发了一种基于一锅级联放大 DNA 电路的逻辑门操作的光子晶体(PC)增强型荧光生物传感器,用于对两种与癌症相关的 miRNA 进行无酶超灵敏分析。该荧光生物传感器通过生化识别放大模块(BCRAM)和物理增强模块(PEM)实现逻辑和灵敏检测。在 BCRAM 中,一锅级联放大电路由上游并联熵驱动电路(EDC)和下游共享催化发夹组件(CHA)组成。目标 miRNA 的输入会触发每个相应的 EDC,并行的 EDC 释放出相同的 R 链以触发后续的 CHA,从而获得 OR 逻辑门,实现了设计和操作的最小化。在 PEM 中,光子晶体(PC)阵列很容易制备,可通过光学调制功能特异性地增强 BCRAM 的荧光输出;同时,微孔板分析仪可实现高通量信号读取。得益于两个模块的集成优势,所提出的生物传感器实现了对两种 miRNA 的超灵敏检测,逻辑门操作简单,在等温和无酶条件下的 LOD 分别为 8.6 fM 和 6.7 fM。因此,该生物传感器具有灵敏度高、操作简便、多路复用和高通量分析等优点,在癌症早期筛查方面具有巨大潜力。
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引用次数: 0
Platinum single atom on CsPbBr3 nanocrystals as electrocatalyst boosts electrochemical sensing of ascorbic acid. CsPbBr3 纳米晶体上的铂单原子作为电催化剂促进了抗坏血酸的电化学传感。
IF 5.6 1区 化学 Q1 CHEMISTRY, ANALYTICAL Pub Date : 2024-09-01 Epub Date: 2024-06-18 DOI: 10.1016/j.talanta.2024.126396
Yuanlong Qin, Hanzhang Chen, Yu Luo, Jiayue Zhang, Kejia Zhou, Yumin Leng, Jia Zheng, Zhengbo Chen

Monitoring ascorbic acid (AA) levels in human body can provide valuable clues for disease diagnosis. Anchoring noble metal single atoms on perovskite substrate is a promising strategy to design electrocatalysts with outstanding electrocatalytic performance. Herein, we design an electrochemical method for detecting AA by utilizing Pt single atoms-doped CsPbBr3 nanocrystals (Pt SA/CsPbBr3 NCs) fixed on a glassy carbon electrode as an electrochemical catalyst. The uncharged 3,5,3',5'-tetramethylbenzidine (TMB) undergoes oxidation to form the positively charged oxidized TMB (oxTMB) owing to the exceptional electrochemical catalytic performance of Pt SA/CsPbBr3 NCs. Subsequently, the target AA reduces oxTMB to TMB, which is then electrocatalytically oxidized to oxTMB, producing significant oxidation current. In this way, such characteristic provides a sensitive electrochemical strategy for AA detection, achieving a concentration range of 50-fold with the detection limit of 0.0369 μM. The developed electrochemical method also successfully generates accurate detection response of AA in complex sample media (urine). Overall, this approach is expected to offer a novel way for early disease diagnosis.

监测人体内的抗坏血酸(AA)水平可为疾病诊断提供有价值的线索。在过氧化物基底上锚定贵金属单原子是设计具有出色电催化性能的电催化剂的一种有前途的策略。在此,我们利用固定在玻璃碳电极上的掺杂铂单原子的 CsPbBr3 纳米晶体(铂 SA/CsPbBr3 NCs)作为电化学催化剂,设计了一种检测 AA 的电化学方法。由于 Pt SA/CsPbBr3 NCs 具有优异的电化学催化性能,不带电的 3,5,3',5'-四甲基联苯胺(TMB)会被氧化,生成带正电的氧化 TMB(oxTMB)。随后,目标 AA 将 oxTMB 还原成 TMB,TMB 再通过电催化氧化成 oxTMB,从而产生巨大的氧化电流。因此,这种特性为 AA 检测提供了一种灵敏的电化学策略,其浓度范围可达 50 倍,检测限为 0.0369 μM。所开发的电化学方法还成功地在复杂的样品介质(尿液)中产生了准确的 AA 检测响应。总之,这种方法有望为早期疾病诊断提供一种新的途径。
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引用次数: 0
A handheld fluorescent lateral flow immunoassay platform for highly sensitive point-of-care detection of methamphetamine and tramadol. 用于高灵敏度床旁检测甲基苯丙胺和曲马多的手持式荧光侧流免疫分析平台。
IF 5.6 1区 化学 Q1 CHEMISTRY, ANALYTICAL Pub Date : 2024-09-01 Epub Date: 2024-06-18 DOI: 10.1016/j.talanta.2024.126438
Weijia Li, Xingsheng Yang, Dongfeng Wang, Jianwei Xie, Shengqi Wang, Zhen Rong

The escalating issue of drug abuse poses a significant threat to public health and societal stability worldwide. An on-site drug detection platform is vital for combating drug abuse and trafficking, as it eliminates the need for additional tools, extensive processes, or specialized training. Therefore, it is imperative to develop a fast, sensitive, non-invasive, and reliable multiplex drug testing platform. In this study, we have presented a silica core@dual quantum dot-shell nanocomposite (SI/DQD)-based fluorescent lateral flow immunoassay (LFIA) platform for the highly sensitive and simultaneous point-of-care (POC) detection of methamphetamine (MET) and tramadol (TR). A 3D-printed attachment was designed to integrate optical and electrical components, facilitating the miniaturization of the instrument and reducing both cost and complexity. The device's advanced hardware and effective fluorescence extraction algorithm with waveform reconstruction enable swift, automatic noise reduction and data analysis. SI/DQD nanocomposites were utilized as fluorescent nanotags in the LFIA strips due to their outstanding luminous efficiency and robustness. This LFIA platform achieves impressive detection limits (LODs) of 0.11 ng mL-1 for MET and 0.017 ng mL-1 for TR. The method has also successfully detected MET and TR in complex biological samples, demonstrating its practical application capabilities. The proposed fluorescent LFIA platform, based on SI/DQD technology, holds significant promise for the swift and accurate POC detection of these substances. Its affordability, compact size, and excellent analytical performance make it suitable for on-site drug testing, including at borders and roadside checks, and open up new possibilities for the design and implementation of drug testing methods.

不断升级的药物滥用问题对全球公共卫生和社会稳定构成了重大威胁。现场药物检测平台对于打击药物滥用和贩运至关重要,因为它无需额外的工具、广泛的流程或专业培训。因此,开发一种快速、灵敏、无创、可靠的多重毒品检测平台势在必行。在这项研究中,我们提出了一种基于硅核@双量子点-贝壳纳米复合材料(SI/DQD)的荧光侧向流免疫分析(LFIA)平台,用于高灵敏度、同时在床旁点(POC)检测甲基苯丙胺(MET)和曲马多(TR)。设计的三维打印附件集成了光学和电子元件,促进了仪器的微型化,降低了成本和复杂性。该设备的先进硬件和有效的荧光提取算法以及波形重建功能可实现快速、自动降噪和数据分析。SI/DQD 纳米复合材料因其出色的发光效率和坚固性被用作 LFIA 条带中的荧光纳米标签。该 LFIA 平台实现了令人印象深刻的检测限 (LOD):MET 为 0.11 ng mL-1,TR 为 0.017 ng mL-1。该方法还成功检测了复杂生物样本中的 MET 和 TR,证明了其实际应用能力。基于 SI/DQD 技术的拟议荧光 LFIA 平台在快速、准确地 POC 检测这些物质方面前景广阔。它价格低廉、体积小巧、分析性能卓越,适用于现场药物检测,包括边境和路边检查,为药物检测方法的设计和实施开辟了新的可能性。
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引用次数: 0
Bifunctional Tb(III)-modified Ce-MOF nanoprobe for colorimetric and fluorescence sensing of α-glucosidase activity. 用于α-葡萄糖苷酶活性比色和荧光传感的双功能锑(III)修饰 Ce-MOF 纳米探针。
IF 5.6 1区 化学 Q1 CHEMISTRY, ANALYTICAL Pub Date : 2024-08-15 Epub Date: 2024-05-22 DOI: 10.1016/j.talanta.2024.126304
Yi Xiao, Pengcheng Huang, Fang-Ying Wu

α-Glucosidase, which directly involves in the metabolism of starch and glycogen and causes an increase in blood sugar level, is the major target enzyme for the precaution and therapy of type II diabetes. Based on the previous work, we adopted a post-synthetic modification method to encapsulate Tb3+ into Ce-MOF nanozyme which owned mixed valence states. Tb@Ce-MOF displayed induced luminescence characteristic and exceptional oxidase-like activity that could oxidize colorless 3,3',5,5'-tetramethylbenzidine (TMB) to blue ox-TMB. α-Glucosidase can hydrolyze the substrate l-ascorbic acid-2-O-α-d-glucopyranosyl (AAG) to generate ascorbic acid (AA), which could increase the Ce3+/Ce4+ redox valence mode in Tb@Ce-MOF, leading to the inhibition of the allochroic reaction of TMB and the decreased absorption of ox-TMB at 652 nm. The energy transfer (EnT) process from Ce3+ to Tb3+ will enhance due to the increased Ce3+/Ce4+ mode in Tb@Ce-MOF, which will result in an enhanced fluorescence signal of Tb@Ce-MOF at 550 nm. But the addition of inhibitor acarbose will inhibit the above process. We have constructed a dual-mode detection platform of α-glucosidase and its inhibitor via colorimetric and fluorometric method. The linear range of α-glucosidase were 0.01-0.5 U/mL (colorimetric mode) and 0.8-1.5 U/mL (fluorometric mode), respectively, with a detection limit as low as 0.0018 U/mL. Furthermore, our approach was also successfully employed to the analysis of α-glucosidase in serum samples.

α-葡萄糖苷酶直接参与淀粉和糖原的代谢,导致血糖升高,是预防和治疗Ⅱ型糖尿病的主要靶酶。在前人工作的基础上,我们采用后合成修饰的方法将 Tb3+ 封装到具有混合价态的 Ce-MOF 纳米酶中。Tb@Ce-MOF具有诱导发光特性和特殊的氧化酶样活性,能将无色的3,3',5,5'-四甲基联苯胺(TMB)氧化成蓝色的ox-TMB。α-葡萄糖苷酶能水解底物 l-抗坏血酸-2-O-α-d-吡喃葡萄糖基(AAG)生成抗坏血酸(AA),从而增加 Tb@Ce-MOF 中的 Ce3+/Ce4+ 氧化还原价态,导致 TMB 的异色反应受到抑制,ox-TMB 在 652 纳米波长处的吸收降低。由于 Tb@Ce-MOF 中 Ce3+/Ce4+ 模式的增加,从 Ce3+ 到 Tb3+ 的能量转移(EnT)过程将增强,这将导致 Tb@Ce-MOF 在 550 纳米波长处的荧光信号增强。但加入抑制剂阿卡波糖会抑制上述过程。我们通过比色法和荧光法构建了α-葡萄糖苷酶及其抑制剂的双模式检测平台。α-葡萄糖苷酶的线性范围分别为 0.01-0.5 U/mL(比色法)和 0.8-1.5 U/mL(荧光法),检测限低至 0.0018 U/mL。此外,我们的方法还成功地应用于血清样品中α-葡萄糖苷酶的分析。
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引用次数: 0
A highly sensitive nanopore platform for measuring RNase A activity. 用于测量 RNase A 活性的高灵敏度纳米孔平台。
IF 5.6 1区 化学 Q1 CHEMISTRY, ANALYTICAL Pub Date : 2024-08-15 Epub Date: 2024-05-22 DOI: 10.1016/j.talanta.2024.126276
Haiyan Zheng, Sathishkumar Munusamy, Pearl Arora, Rana Jahani, Xiyun Guan

Ribonuclease A (RNase A) plays significant roles in several physiological and pathological conditions and can be used as a valuable diagnostic biomarker for human diseases such as myocardial infarction and cancer. Hence, it is of great importance to develop a rapid and cost-effective method for the highly sensitive detection of RNase A. The significance of RNase A assay is further enhanced by the growing attention from the biotechnology and pharmaceutical industries to develop RNA-based vaccines and drugs in large part as a result of the successful development of mRNA vaccines in the COVID-19 pandemic. Herein, we report a label-free method for the detection of RNase A by monitoring its proteolytic cleavage of an RNA substrate in a nanopore. The method is ultra-sensitive with the limit of detection reaching as low as 30 fg per milliliter. Furthermore, sensor selectivity and the effects of temperature, incubation time, metal ion, salt concentration on sensor sensitivity were also investigated.

核糖核酸酶 A(RNase A)在多种生理和病理状态下发挥着重要作用,可用作心肌梗塞和癌症等人类疾病的重要诊断生物标志物。由于在 COVID-19 大流行中成功开发了 mRNA 疫苗,生物技术和制药行业越来越关注开发基于 RNA 的疫苗和药物,这进一步增强了 RNase A 检测的重要性。在此,我们报告了一种通过监测 RNase A 在纳米孔中对 RNA 底物的蛋白水解作用来检测 RNase A 的无标记方法。该方法灵敏度极高,检测限低至每毫升 30 fg。此外,还研究了传感器的选择性以及温度、孵育时间、金属离子、盐浓度对传感器灵敏度的影响。
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引用次数: 0
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