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IEEE Sensors Council

IF 4.3 2区综合性期刊 Q1 ENGINEERING, ELECTRICAL & ELECTRONIC

IEEE Sensors Journal

Pub Date : 2024-11-27 DOI: 10.1109/JSEN.2024.3498799

引用次数: 0

Pulsed Electromagnetic Field-Assisting Reduced Graphene Oxide-Incorporated Nanofibers for Osteogenic Differentiation of Human Dental Pulp Stem Cells 用于人牙髓干细胞成骨分化的脉冲电磁场辅助还原石墨烯氧化物纳米纤维

IF 1.8 Q3 MATERIALS SCIENCE, MULTIDISCIPLINARY

IEEE Open Journal of Nanotechnology

Pub Date : 2024-11-27 DOI: 10.1109/OJNANO.2024.3494770

Juo Lee;Sungmin Lee;Iksong Byun;Myung Chul Lee;Jungsil Kim;Hoon Seonwoo

In bone tissue engineering, various approaches have been investigated to enhance osteogenic regeneration. Previous studies have predominantly employed scaffolds with aligned structures or reduced graphene oxide (RGO) to facilitate bone regeneration. However, current scaffold designs face limitations in combining structural guidance with effective electromagnetic stimulation. Additionally, delivering localized stimulation within scaffolds remains a challenge in maximizing the potential of these materials for bone regeneration. To address these limitations and strengthen previous approaches, this study presents a novel strategy in tissue engineering for enhanced osteogenic differentiation. RGO-incorporated nanofibers (RGO-NFs) were fabricated via electrospinning a 10% polycaprolactone (PCL) solution with RGO concentrations varying. The random fibers were deposited on a planar surface, while the aligned fibers were deposited on a rotating drum. The morphology and orientation of the fibers were confirmed through electron microscopy. X-ray diffraction spectrometry was employed to confirm the integration of RGO and PCL. All groups demonstrated optimal cell adhesion and viability. RGO-NFs exhibited higher osteogenesis-related protein expression than PCL-only scaffolds, further enhanced by pulsed electromagnetic field (PEMF) application. The application of PEMF stimulation within aligned RGO-NFs presents a potentially more efficient alternative to existing methods, offering a novel, non-invasive therapeutic strategy for bone defect regeneration.

在骨组织工程中，人们研究了各种方法来促进成骨再生。以往的研究主要采用具有排列结构或还原氧化石墨烯（RGO）的支架来促进骨再生。然而，目前的支架设计在将结构引导与有效电磁刺激相结合方面存在局限性。此外，在支架内提供局部刺激仍是最大限度发挥这些材料骨再生潜力的一个挑战。为了解决这些局限性并加强以前的方法，本研究提出了一种组织工程中增强成骨分化的新策略。研究人员通过电纺10%的聚己内酯（PCL）溶液（RGO浓度各不相同）来制造RGO掺杂纳米纤维（RGO-NFs）。随机纤维沉积在平面上，而排列整齐的纤维则沉积在旋转的滚筒上。纤维的形态和取向通过电子显微镜进行了确认。X 射线衍射光谱法用于确认 RGO 和 PCL 的整合。所有组都显示出最佳的细胞粘附性和存活率。与仅使用 PCL 的支架相比，RGO-NFs 表现出更高的成骨相关蛋白表达量，应用脉冲电磁场（PEMF）进一步增强了这种表达量。在排列整齐的 RGO-NFs 中应用 PEMF 刺激可能是现有方法的一种更有效的替代方法，为骨缺损再生提供了一种新颖、非侵入性的治疗策略。

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引用次数: 0

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