Bioelectrochemistry最新文献

Precise detection of telomerase reverse transcriptase C228T mutation using label-free and magnetically induced self-assembly electrochemical biosensors. 利用无标记和磁诱导自组装电化学生物传感器精确检测端粒酶逆转录酶C228T突变。

IF 4.9 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2026-10-19 DOI: 10.1016/j.bioelechem.2026.109473

Wentao Li, Min Liu, Zhanyi Qu, Qianqian Deng, Ruijiang Liu, Zhan-Ao Wu

Telomerase reverse transcriptase promoter C228T mutations (TERT_C228T) are strongly associated with poor outcomes in papillary thyroid cancer, necessitating sensitive detection methods. In this work, a "Signal-off" type of label-free and magnetically induced self-assembly electrochemical biosensors based on α-Fe₂O₃/Fe₃O₄@Au magnetic nanocomposites (MNCs) were developed for TERT_C228T detection. Thiolated aptamers (Apts) for specific recognition of TERT_C228T were immobilized on α-Fe₂O₃/Fe₃O₄@Au MNCs via AuS bonds, and bovine serum albumins (BSA) were employed to block non-specific sites and form α-Fe₂O₃/Fe₃O₄@Au-Apt/BSA biosensors. TERT_C228T specifically hybridized with Apts, forming a rigid dsDNA film on the electrode. Enhanced steric hindrance and electrostatic repulsion suppressed electron transfer of [Fe(CN)₆]^3-/4-, leading to decreased current signals. The biosensors were evaluated by cyclic voltammetry, electrochemical impedance spectroscopy, and differential pulse voltammetry, the results demonstrated that the biosensors exhibited a wide linear range (10² pM- 10⁶ pM), high sensitivity (LOD of 0.67 pM), and excellent selectivity, along with high reproducibility (RSD = 1.84%) and long-term stability (signal retention above 95% within 10 days), and enabled reliable detection in diluted human serum (recoveries of 98.1%-107.3%). Overall, the electrochemical biosensors offer a simple, rapid, and ultrasensitive platform for TERT_C228T detection, with promising potential for molecular diagnosis and thyroid cancer risk stratification.

端粒酶逆转录酶启动子C228T突变（TERTC228T）与甲状腺乳头状癌预后不良密切相关，需要灵敏的检测方法。在这项工作中，基于α-Fe2O3/Fe3O4@Au磁性纳米复合材料（MNCs）开发了一种“信号关闭”型无标签磁诱导自组装电化学生物传感器，用于TERTC228T检测。将用于TERTC228T特异性识别的硫代适配体（Apts）通过AuS键固定在α-Fe2O3/Fe3O4@Au MNCs上，利用牛血清白蛋白（BSA）阻断非特异性位点，形成α-Fe2O3/Fe3O4@Au-Apt/BSA生物传感器。TERTC228T与Apts特异性杂交，在电极上形成刚性的dsDNA膜。空间位阻和静电斥力的增强抑制了[Fe(CN)6]3-/4-的电子转移，导致电流信号减弱。通过循环伏安法、电化学阻抗谱法和差分脉冲伏安法对该传感器进行了评价，结果表明，该传感器具有宽线性范围（102 pM ~ 106 pM）、高灵敏度（LOD为0.67 pM）、优良的选择性、高重复性（RSD = 1.84%）和长期稳定性（10 d内信号保留率在95%以上），并能在稀释后的人血清中可靠检测（回收率为98.1% ~ 107.3%）。总的来说，电化学生物传感器为TERTC228T的检测提供了一个简单、快速、超灵敏的平台，在分子诊断和甲状腺癌风险分层方面具有很大的潜力。

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引用次数: 0

Microalgae cells with extracellular gold nanoparticles for enhanced photobioelectrochemical activity 微藻细胞与细胞外金纳米粒子增强光生物电化学活性

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2026-10-01 Epub Date: 2026-03-04 DOI: 10.1016/j.bioelechem.2026.109264

Caio C.G. Silva , Alessandro Cacia , Hernán D. Rojas-Mantilla , Maria V.B. Zanoni , Saulo S. Garrido , Felipe Conzuelo

Photobioelectrochemical systems (PBEs) harness the machinery of photosynthetic microorganisms to convert solar energy into electricity. However, the inefficient electron transfer at the cell-electrode interface remains as the key performance limitation. Herein, we report a plasmonic biohybrid strategy to enhance extracellular electron transfer in Chlorella-based photobioelectrodes by integrating gold nanoparticles (AuNPs) localized on the microalgae membrane. Two approaches are investigated, consisting of physical mixing of isolated cells with AuNPs and cultivating microalgae in a growth medium supplemented with AuNPs, with the second approach allowing to obtain about significantly higher photocurrent responses. Optimized bioelectrodes yield photocurrent densities of up to 132 μA cm⁻², 74% higher than bioelectrodes fabricated using unmodified cells. Spectral response analysis reveals a strong resonance at 525 nm, consistent with the plasmonic properties of AuNPs. Importantly, pigment content, growth kinetics, and membrane integrity are preserved, confirming the biocompatibility of the modification. This work presents a facile and effective route to engineer photosynthetic bioelectrodes using nanomaterials, advancing the design of high-performance PBEs for solar-to-electricity conversion.

光生物电化学系统（PBEs）利用光合微生物的机制将太阳能转化为电能。然而，电池-电极界面的低效率电子转移仍然是主要的性能限制。在此，我们报道了一种等离子体生物杂交策略，通过整合定位在微藻膜上的金纳米颗粒（AuNPs）来增强小球藻基光生物电极的细胞外电子转移。研究人员研究了两种方法，一种是将分离细胞与AuNPs物理混合，另一种是在添加了AuNPs的生长培养基中培养微藻，第二种方法可以获得更高的光电流响应。优化后的生物电极产生的光电流密度高达132 μA cm−2，比使用未修饰的细胞制备的生物电极高74%。光谱响应分析显示在525 nm处有强烈的共振，与AuNPs的等离子体特性一致。重要的是，色素含量、生长动力学和膜完整性都得到了保存，证实了改性的生物相容性。这项工作提出了一种简单有效的途径来利用纳米材料设计光合生物电极，推进了高性能PBEs的设计，用于太阳能到电力的转换。

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引用次数: 0

Anthraquinone disulfonate as a stable redox mediator for efficient air-cathodes at neutral pH in dual-chamber microbial fuel cells 二磺酸蒽醌作为双室微生物燃料电池中性pH下高效空气阴极的氧化还原介质

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2026-10-01 Epub Date: 2026-03-09 DOI: 10.1016/j.bioelechem.2026.109279

Antoine Vautier, James A. Behan, Charlotte Bodin, Florence Geneste, Frédéric Barrière

Microbial Fuel Cells (MFCs) are commonly developed as organic-matter oxidizing bioanodes with abiotic air cathodes. However, O₂ reduction requires active aeration and/or the use of expensive catalysts using noble metals. In this study, 2,7-anthraquinone disulfonate (2,7-AQDS), an organic redox mediator commonly used in aqueous redox flow battery systems (AORFBs), served as a redox-stable intermediate for oxygen reduction. Dual-chamber MFC pilots were developed with 2,7-AQDS in the catholyte under both anoxic and aerobic conditions and compared to pilots with ferricyanide catholytes. In both conditions, cyclic voltammetry studies confirmed similar and efficient electroactivity despite the proximity AQDS formal redox potential to that of acetate oxidation. Mediated air-cathodes achieved open-circuit voltage (OCV) of 510 mV and current densities of 140 μA/cm², nearly double those of air-only cathodes (72 μA/cm²), while delivering a 33% higher power density (12 mW/m² vs. 8 mW/m²). Passive catholyte aeration enabled continuous reoxidation of reduced 2,7-AQDS at 8.8 × 10⁻⁸ mol/s, exceeding the AQDS reduction rate by the bioanode (2.5 × 10⁻¹⁰ mol/s), thus ensuring effective self-regeneration and stable AQDS concentration. These results demonstrate that AQDS coupled with passive oxygen supply sustains biofilm activity with enhances current and power, and allow long-term / low-maintenance MFC operation and organic-matter oxidation.

微生物燃料电池（mfc）通常被开发为有机物氧化生物阳极和非生物空气阴极。然而，O2还原需要主动曝气和/或使用昂贵的贵金属催化剂。在本研究中，2,7-蒽醌二磺酸盐（2,7- aqds）作为水氧化还原液流电池系统（AORFBs）中常用的有机氧化还原介质，作为氧还原的氧化还原稳定中间体。在缺氧和有氧条件下培养具有2,7- aqds的双室MFC飞行员，并与具有铁氰化阴极的飞行员进行比较。在这两种情况下，循环伏安法研究证实，尽管AQDS形式氧化还原电位与醋酸氧化电位接近，但电活性相似且有效。介质空气阴极的开路电压（OCV）为510 mV，电流密度为140 μA/cm2，几乎是纯空气阴极（72 μA/cm2）的两倍，而功率密度（12 mW/m2 vs. 8 mW/m2）提高了33%。被动阴极曝气使还原的2,7-AQDS以8.8 × 10−8 mol/s的速度连续再氧化，超过了生物阳极对AQDS的还原速率（2.5 × 10−10 mol/s），从而保证了有效的自再生和稳定的AQDS浓度。这些结果表明，AQDS与被动供氧相结合，可以在增强电流和功率的情况下维持生物膜的活性，并允许长期/低维护的MFC运行和有机物氧化。

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引用次数: 0

Developing fast scan cyclic voltammetry at carbon fiber microelectrodes to quantify short chain fatty acids in situ 碳纤维微电极快速扫描循环伏安法原位定量短链脂肪酸。

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2026-10-01 Epub Date: 2026-03-04 DOI: 10.1016/j.bioelechem.2026.109266

Terdha Narla , Uma Nudurupati, Yangguang Ou

Acetic, propionic, and butyric acids are short chain fatty acids (SCFAs) and the most abundant metabolites produced by gut bacteria. To uncover mechanistic insights of their function throughout the body, it is critical to measure rapid SCFA fluxes in sites of action such as brain, muscle, and skin. Current approaches have focused on fecal and plasma measurements, where SCFA levels are low and not representative of tissue-specific concentrations or fluxes. Thus, a rapid, in situ methodology is needed. Fast scan cyclic voltammetry (FSCV) at carbon fiber microelectrodes (CFMs) has the spatiotemporal resolution to fill this need. However, the electrochemical response of CFMs to SCFAs have not been explored until now. By coupling FSCV at CFMs with flow injection analysis of each SCFA, we demonstrate there are distinct peaks in the cyclic voltammograms for each fatty acid. Some of these peaks display faradaic behavior in scan rate, holding potential, and switching potential experiments. Interestingly, we identify several peaks that are concentration-sensitive and therefore are promising quantifiable markers of SCFA dynamics and fluxes. This work lays the foundation in understanding the response of CFMs to fatty acids and demonstrate the utility of FSCV at CFMs for the in situ quantitation of SCFAs.

乙酸、丙酸和丁酸是短链脂肪酸（SCFAs），是肠道细菌产生的最丰富的代谢物。为了揭示其在全身功能的机制，测量脑、肌肉和皮肤等作用部位的快速SCFA通量至关重要。目前的方法主要集中在粪便和血浆测量，其中SCFA水平较低，不能代表组织特异性浓度或通量。因此，需要一种快速的就地方法。碳纤维微电极（CFMs）的快速扫描循环伏安法（FSCV）具有满足这一需求的时空分辨率。然而，cfm对scfa的电化学响应目前还没有研究。通过将cfm的FSCV与每种SCFA的流动注射分析相结合，我们发现每种脂肪酸的循环伏安图中都有不同的峰。其中一些峰在扫描速率、保持电位和开关电位实验中显示出法拉第行为。有趣的是，我们发现了几个对浓度敏感的峰，因此是有希望的SCFA动态和通量的可量化标记。这项工作为理解cfm对脂肪酸的反应奠定了基础，并证明了FSCV在cfm中用于scfa原位定量的实用性。

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引用次数: 0

Nanotip acetylcholine biosensor reveals cholinergic differentiated SH-SY5Y cells release partial vesicle content during exocytosis 纳米尖端乙酰胆碱生物传感器显示胆碱能分化的SH-SY5Y细胞在胞吐过程中释放部分囊泡内容物

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2026-10-01 Epub Date: 2026-02-26 DOI: 10.1016/j.bioelechem.2026.109260

Yuanmo Wang , Ajay Pradhan , Pankaj Gupta , Jörg Hanrieder , Henrik Zetterberg , Ann-Sofie Cans

Acetylcholine (ACh) is a central neurotransmitter in cognitive function, motor control, and synaptic modulation, yet its electrochemical inactivity and the rapid kinetics of exocytosis have hindered real-time quantal measurements. Micrometer-scale enzymatic ACh biosensors previously enabled sub-millisecond extracellular recordings but were too large for synaptic positioning and intracellular recordings. Here we present a short, ultrafast and low-noise amperometric ACh biosensor based on a needle-shaped carbon fiber nanotip electrode functionalized with gold nanoparticles and enzymes. The miniaturized geometry allows precise placement at neurite release sites and minimally invasive insertion into the cell cytoplasm, enabling high-temporal resolution monitoring of presynaptic exocytosis together with quantification of intracellular ACh vesicle content. We applied this platform to differentiated human cholinergic SH-SY5Y neuroblastoma cells, an established yet underutilized cell model for cholinergic signaling. The nanotip sensor successfully captured amperometric spikes from both intracellular vesicle burst events and presynaptic ACh release. Intracellular events released a larger amount of ACh than presynaptic exocytosis events, indicating a predominance of partial exocytosis mode in these cells. These results demonstrate the nanotip ACh biosensor as a unique tool for probing fusion pore dynamics at subcellular resolution and for providing quantitative insight into the quantal nature of cholinergic signaling in human neuronal models.

乙酰胆碱（ACh）是认知功能、运动控制和突触调节的中枢神经递质，但它的电化学不活性和胞吐的快速动力学阻碍了实时量子测量。微米级的酶促ACh生物传感器以前可以进行亚毫秒级的细胞外记录，但对于突触定位和细胞内记录来说太大了。本文提出了一种短、超快、低噪声的基于针状碳纤维纳米电极的ACh生物传感器，该电极具有金纳米粒子和酶的功能。小型化的几何结构允许精确放置在神经突释放位点，并微创插入细胞质，从而实现突触前胞吐的高时间分辨率监测以及细胞内ACh囊泡含量的定量。我们将该平台应用于分化的人胆碱能SH-SY5Y神经母细胞瘤细胞，这是一种已建立但未充分利用的胆碱能信号传导细胞模型。纳米尖端传感器成功捕获了细胞内囊泡破裂事件和突触前乙酰胆碱释放的电流峰值。细胞内事件比突触前胞吐事件释放更多的乙酰胆碱，表明这些细胞以部分胞吐模式为主。这些结果表明，纳米尖端ACh生物传感器是一种独特的工具，可以在亚细胞分辨率下探测融合孔动力学，并为人类神经元模型中胆碱能信号的量子性质提供定量的见解。

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引用次数: 0

Electrochemistry of redox enzymes: from functional enzyme immobilization to enzymatic bio-electrochemical devices (tutorial) 氧化还原酶的电化学：从功能酶固定化到酶生物电化学装置（教程）

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2026-10-01 Epub Date: 2026-03-04 DOI: 10.1016/j.bioelechem.2026.109265

A. Guessab, I. Mazurenko, E. Lojou, A. de Poulpiquet

Electrochemistry of redox enzymes is a multidisciplinary field, and students who join labs specialized in bio-electrochemistry have various backgrounds: chemistry, biology, biotechnologies, nanotechnologies, physics, etc., so that their level in the involved disciplines is often heterogeneous, and they sometimes lack the basic knowledge about either of the fields concerned by their project (physical chemistry, electrochemistry, enzymology, etc.). Projects that gather experienced researchers from these different areas also sometimes suffer from a lack of understanding between the partners. This tutorial, which follows a lecture given at the first winter school of the French group of bio-electrochemistry (GFB), aims at guiding newcomers in the field and provides advice for more in-depth and specialized literature. It seeks to provide a solid theoretical and experimental foundation. Redox enzymes and the basic methods of immobilization at the electrode are introduced. The mechanisms of direct and mediated electron transfers are explained. An important electrochemical method, cyclic voltammetry, is explained, and the article describes how to extract information about enzyme/electrode and catalytic reactions. Finally, the tutorial presents two enzymatic bio-electrochemical devices, enzymatic fuel cells and enzymatic biosensors, and provides a guide for their electrochemical characterization.

氧化还原酶的电化学是一个多学科领域，加入生物电化学实验室的学生有不同的背景：化学、生物学、生物技术、纳米技术、物理学等，因此他们在所涉及的学科中的水平往往是异质的，有时他们缺乏项目所涉及的任何一个领域（物理化学、电化学、酶学等）的基础知识。从这些不同领域聚集经验丰富的研究人员的项目有时也会因合作伙伴之间缺乏理解而受到影响。本教程是继法国生物电化学小组（GFB）第一届冬季学校的讲座之后编写的，旨在指导该领域的新手，并为更深入和专业的文献提供建议。它力求提供一个坚实的理论和实验基础。介绍了氧化还原酶及其电极固定化的基本方法。解释了直接和间接电子转移的机理。介绍了一种重要的电化学方法——循环伏安法，并介绍了如何提取酶/电极和催化反应的信息。最后，本教程介绍了两种酶生物电化学装置，酶燃料电池和酶生物传感器，并提供了它们的电化学表征指南。

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引用次数: 0

Proof-of-concept bioelectrochemical characterization of Mycolicibacterium smegmatis for diagnostic applications 耻垢分枝杆菌诊断应用的概念验证生物电化学表征

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2026-10-01 Epub Date: 2026-03-09 DOI: 10.1016/j.bioelechem.2026.109277

Sunday Olakunle Oguntomi , Abhipsa Sahu , Yili Yang , Enrico Marsili

Elevated urease activity in tuberculosis-infected individuals is often suggestive of bacterial virulence by promoting survivability and establishment of the causative pathogen (Mycobacterium tuberculosis) in the host. Thus, heightened urease activity could be a promising biomarker for screening pathogenic mycobacterial strains. In this proof-of-concept study, we evaluate the suitability of electrochemical techniques for direct screening of mycobacterial strains based on urease activity. Mycolicibacterium smegmatis wildtype (WT), a derived knockout mutant of the LCP protein family (∆0107) with high urease activity, and its complement strain (c-0107) were coated in polydopamine (PDA) and immobilized on a screen-printed electrode (SPE) for microscopy and electrochemical characterization, in presence of exogenous redox mediators: potassium ferricyanide, anthraquinone, and riboflavin. Microscopy showed compatibility of PDA as a coating material for mycobacterial cells. Electrochemical analysis results indicated enhanced M. smegmatis electroactivity in the presence of anthraquinone, with higher current output in the strain with high urease activity. Experiments at different urea concentrations suggest that urea is not required for this screening approach. This work shows that electrochemical data can potentially distinguish mycobacterial strains based on urease activity. It also lays a groundwork in the model mycobacteria M. smegmatis, which could facilitate development of assays for pathogenic mycobacterial species.

结核菌感染个体的脲酶活性升高，通常通过促进宿主的生存能力和致病病原体（结核分枝杆菌）的建立，提示细菌的毒力。因此，提高脲酶活性可能是筛选致病性分枝杆菌菌株的一个有希望的生物标志物。在这项概念验证研究中，我们评估了基于脲酶活性的电化学技术直接筛选分枝杆菌菌株的适用性。在外源性氧化还原介质铁氰化钾、蒽醌和核黄素的存在下，将LCP蛋白家族（∆0107）的敲除突变体——耻毛分枝杆菌野生型（WT）及其补体菌株（c-0107）包被聚多巴胺（PDA），并固定在屏幕印刷电极（SPE）上进行显微镜和电化学表征。显微镜显示PDA作为分枝杆菌细胞包衣材料的相容性。电化学分析结果表明，蒽醌的存在增强了耻垢分枝杆菌的电活性，且菌株具有较高的脲酶活性。不同尿素浓度的实验表明，这种筛选方法不需要尿素。这项工作表明电化学数据可以根据脲酶活性潜在地区分分枝杆菌菌株。这也为建立耻垢分枝杆菌模型奠定了基础，为开发致病性分枝杆菌的检测方法奠定了基础。

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引用次数: 0

Optimization of a MWCNTs-AuNPs composite-modified electrochemical immunosensor with anti-fouling property for highly sensitive and rapid detection of BNP in myocardial infarction-induced heart failure diagnosis MWCNTs-AuNPs复合修饰抗污染电化学免疫传感器在心肌梗死性心力衰竭诊断中的高灵敏度和快速检测

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2026-10-01 Epub Date: 2026-02-28 DOI: 10.1016/j.bioelechem.2026.109258

Jiaru Fu , Dewei Huang , Bolu Sun , Chengwen Li , Yuqiong Wu , Huajie Li , Jiali Kang , Haiying He , Xiangdong Wang , Yaru Zhang , Xuanxiu Da , Mian Chen , Lin Yang

Brain natriuretic peptide (BNP) is a sensitive biomarker for acute heart failure (AHF). Rapid, sensitive, and anti-fouling BNP detection in serum remains clinically unmet. Herein, a novel electrochemical immunosensor with synergistic signal amplification and anti-fouling ability was constructed via layered AuNPs/MWCNTs-AuNPs modification. Bottom AuNPs mitigate MWCNTs agglomeration and enable stable Anti-BNP immobilization via AuS bonds, MWCNTs enhance conductivity, and top AuNPs amplify signals, forming an efficient electron-transport network. A nitrocellulose (NC) membrane was laminated for anti-fouling via rapid contaminant sieving. Under optimization, the sensor shows a linear range of 7.81–8 × 10³ pg/mL for BNP, with a detection limit of 8.14 × 10⁻² pg/mL (3σ, n = 11), outperforming ELISA. It exhibits excellent selectivity (vs. cTn-I, glucose, IL-6), repeatability (RSD = 2.3%), and stability (92.4% retention over 30 days). Serum recovery rates of 102–105% confirm anti-fouling reliability. This cost-effective platform promises POCT for AHF and underpins anti-fouling biosensor development.

脑钠肽（BNP）是急性心力衰竭（AHF）的敏感生物标志物。快速、灵敏、抗污染的血清BNP检测仍未在临床上实现。本文通过层状AuNPs/MWCNTs-AuNPs修饰，构建了一种具有协同信号放大和防污能力的新型电化学免疫传感器。底部AuNPs可减轻MWCNTs团聚并通过AuS键实现稳定的Anti-BNP固定化，MWCNTs可增强电导率，顶部AuNPs可放大信号，形成高效的电子传递网络。采用快速过滤的方法制备了一种硝基纤维素（NC）防污膜。优化后，该传感器对BNP的检测线性范围为7.81 ~ 8 × 103 pg/mL，检出限为8.14 × 10-2 pg/mL (3σ, n = 11)，优于ELISA。它具有优良的选择性（相对于ctn - 1、葡萄糖、IL-6）、重复性（RSD = 2.3%）和稳定性（30天内保留率为92.4%）。102-105%的血清回收率证实了抗污染的可靠性。这个具有成本效益的平台为AHF提供了POCT，并支持了防污生物传感器的开发。

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引用次数: 0

Electrochemical cardiac troponin I immunosensor platform integrated with electrochemically deposited Au nanoparticles decorated PtNi bimetallic sponge-like structures. 电化学沉积金纳米粒子修饰PtNi双金属海绵状结构的电化学心脏肌钙蛋白I免疫传感器平台。

IF 4.9 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2026-09-15 DOI: 10.1016/j.bioelechem.2026.109443

Mustafa Ali Güngör, Merve Yıldırım, Filiz Kuralay

In this study, a novel, reproducible, and stable electrochemical detection platform was developed for the detection of cardiac troponin I (cTnI), one of the most common biomarkers of cardiovascular diseases. The immunosensing platform consisted of antibody functionalized electrochemically gold (Au) deposited sponge-like platinum‑nickel (PtNi)-based nanoparticles modified screen-printed carbon electrodes (SPCEs). A sponge-like surface yielded a high surface area that enabled effective Au deposition and enhanced electrochemical properties through a synergistic effect. Electrochemically deposited Au onto this surface not only improved electrochemical characteristics but also created an environment in which the antibody could settle effectively. The final version of the electrode demonstrated high sensitivity. The sensor (anti-cTnI/Au@Pt-Ni/SPCE) performance was achieved in the linear working range of 0.01 pg/mL-100 ng/mL with a low limit of detection (LOD) value of 8.09 fg/mL using EIS technique. The selectivity studies showed that the proposed electrode had successful results in the presence of important interfering species. Good recovery was obtained in diluted serum samples ranging between 97.3 and 103.6%. Amperometric detection strategy was also evaluated with the metal-based nanoparticles modified electrode, and a low LOD value of 3.93 fg/mL was obtained. The modified electrode was successfully characterized using microscopic, spectroscopic, contact angle, and electrochemical techniques.

在这项研究中，开发了一种新的、可重复的、稳定的电化学检测平台，用于检测心脏肌钙蛋白I (cTnI)，这是心血管疾病最常见的生物标志物之一。该免疫传感平台由抗体功能化的电化学金（Au）沉积海绵状铂镍（PtNi）基纳米颗粒修饰的丝网印刷碳电极（spce）组成。海绵状表面产生了高表面积，可以有效沉积金，并通过协同效应增强电化学性能。电化学镀金不仅改善了表面的电化学特性，而且为抗体的有效沉降创造了良好的环境。最终版本的电极显示出高灵敏度。该传感器（anti-cTnI/Au@Pt-Ni/SPCE）在0.01 pg/mL ~ 100 ng/mL的线性工作范围内性能良好，EIS技术的低检出限为8.09 fg/mL。选择性研究表明，该电极在存在重要干扰物质的情况下取得了成功的结果。在稀释后的血清样品中，回收率在97.3 ~ 103.6%之间。对金属基纳米粒子修饰电极的安培检测策略进行了评价，获得了低LOD值3.93 fg/mL。利用显微、光谱、接触角和电化学技术成功地对修饰电极进行了表征。

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引用次数: 0

Performance and functional flora of simultaneous nitrogen removal, desulfurization and electricity generation in sulfide autotrophic denitrification-anammox-MFC coupled system. 硫化物自养反硝化-厌氧氨氧化- mfc耦合系统同时脱氮、脱硫和发电的性能和功能菌群。

IF 4.9 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2026-09-11 DOI: 10.1016/j.bioelechem.2026.109441

Changzi Guo, Dongchan Xi, Liyuan Shao, Xuemei Chen, Dongting Fan, Yong Zhang, Huimin Liu

In this study, a novel SAD-anammox-MFC coupled system was constructed for simultaneous nitrogen removal, desulfurization, and electricity generation. The optimal total nitrogen (TN) removal efficiency of 82.1% was achieved under the following conditions: a sludge inoculation ratio (SADB:AnAOB) of 1:2, external resistance of 500 Ω, influent sulfide concentration of 300 mg S/L, and hydraulic retention time (HRT) of 60 h. The maximum power density (1406.25 mW/m³) occurred at 300 mg S/L and an HRT of 24 h. A longer HRT promoted complete sulfide oxidation and allowed anammox to become the dominant nitrogen removal pathway, with the highest coulombic efficiency (18.20%) at 60 h. Microbial community analysis revealed that the cathode was enriched with the anammox bacterium Candidatus Brocadia (35%) and denitrifying bacterium unclassified_f_PHOS-HE36 (5%), while the anode was dominated by Thiobacillus (15%-20%) and Sulfuricurvum (10%-15%), forming a stable functional zonation. This study provides a low-carbon and resource-oriented solution for treating sulfur- and nitrogen-containing wastewater.

本研究构建了一种新型的SAD-anammox-MFC耦合系统，用于同时脱氮、脱硫和发电。在以下条件下，总氮（TN）的最佳去除率为82.1%：污泥接种比（SADB:AnAOB）为1:2，外部阻力为500 Ω，进水硫化物浓度为300 mg S/L，水力停留时间（HRT）为60 h。在300 mg S/L， HRT为24 h时，功率密度达到1406.25 mW/m3，较长的HRT促进硫化物完全氧化，厌氧氨氧化成为主要的脱氮途径。微生物群落分析表明，阴极富集厌氧细菌Candidatus Brocadia（35%）和反硝化细菌unclassified_f_PHOS-HE36(5%)，阳极以硫杆菌（15% ~ 20%）和硫杆菌（10% ~ 15%）为主，形成稳定的功能区。本研究为含硫含氮废水的处理提供了一种低碳资源化的解决方案。

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