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Outstanding Reviewers for Analytical Methods in 2023. 2023 年分析方法杰出评审员。
IF 2.7 3区 化学 Q2 CHEMISTRY, ANALYTICAL Pub Date : 2024-07-15 DOI: 10.1039/d4ay90075h

We would like to take this opportunity to thank all of Analytical Methods reviewers for helping to preserve quality and integrity in the chemical science literature. We would also like to highlight our Outstanding Reviewers for Analytical Methods in 2023.

我们想借此机会感谢《分析方法》的所有审稿人,感谢他们帮助维护化学科学文献的质量和完整性。我们还想特别介绍一下 2023 年《分析方法》的杰出审稿人。
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引用次数: 0
AuNPs-BP-MWCNTs-COOH-based electrochemical immunosensor for the determination of deoxynivalenol in wheat flour. 基于 AuNPs-BP-MWCNTs-COOH 的电化学免疫传感器用于测定小麦粉中的脱氧雪腐镰刀菌烯醇。
IF 2.7 3区 化学 Q2 CHEMISTRY, ANALYTICAL Pub Date : 2024-07-15 DOI: 10.1039/d4ay00683f
Weiqiang Li, Xiaoxue Liu, Pianpian He, Wentao Hu, Kaijie Tang, Yangping Wen, Qian Zeng, Huiyi Tang, Yaxuan Lei, Xin Liu

Deoxynivalenol (DON) has drawn considerable attention for its obvious pathogenicity and wide use in agro-products, which cause a potential threat to human health. In this work, an electrochemical immunosensor is developed for the highly sensitive and selective detection of DON in wheat flour using AuNPs-BP-MWCNTs-COOH and antibodies. The AuNPs-BP-MWCNTs-COOH nanocomposite was prepared via an in situ reduction reaction and ultrasonic-assisted liquid-phase exfoliation. The nanocomposite exhibits a larger surface area, decent stability, excellent electron transfer capability, good protein binding capability and prominent specificity. The plentiful carboxyl group on the nanocomposite can bind to the amino group of the antibody, and AuNPs have an affinity for the sulfhydryl group of the antibody, which makes it feasible for the nanocomposite to load the antibody. The peak currents are plotted against the logarithm of DON concentration from 0.002 to 80 ng mL-1 with a limit of detection (LOD) of 0.5 pg mL-1. This approach establishes an effective label-free immunosensor platform for the detection of DON with high sensitivity and selectivity in various food and agricultural products.

脱氧雪腐镰刀菌烯醇(DON)因其明显的致病性和在农副产品中的广泛应用而备受关注,对人类健康造成了潜在威胁。本研究利用 AuNPs-BP-MWCNTs-COOH 和抗体开发了一种电化学免疫传感器,用于高灵敏、高选择性地检测小麦粉中的 DON。AuNPs-BP-MWCNTs-COOH 纳米复合材料是通过原位还原反应和超声辅助液相剥离制备的。该纳米复合材料具有较大的比表面积、良好的稳定性、优异的电子传递能力、良好的蛋白质结合能力和突出的特异性。纳米复合材料上丰富的羧基可以与抗体的氨基结合,而 AuNPs 对抗体的巯基具有亲和力,这使得纳米复合材料负载抗体成为可能。峰值电流与 0.002 至 80 ng mL-1 DON 浓度的对数关系图,检测限 (LOD) 为 0.5 pg mL-1。这种方法建立了一个有效的无标记免疫传感器平台,可用于检测各种食品和农产品中的高灵敏度和高选择性 DON。
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引用次数: 0
A novel hollow-electrode glow discharge mass spectrometry for quantitative analysis of protein content in food 用于定量分析食品中蛋白质含量的新型空心电极辉光放电质谱仪
IF 3.1 3区 化学 Q2 CHEMISTRY, ANALYTICAL Pub Date : 2024-07-13 DOI: 10.1039/d4ay01022a
Min Ren, Yingqi Wan, Jiwen Chen
Protein content in food is an important indicator of nutritional value and food safety.Therefore it is great significance to accurately detect protein content in food. In this paper, a combustion furnace and a novel hollow-electrode glow discharge ion source-quadrupole mass spectrometry(HGD-MS) were designed. Use them constructing a "combustion furnace + mass spectrometry" experimental platform to detect the protein content in food. Five food standard samples were selected for analysis. The food samples were combusted in the combustion furnace at a high temperature (1300°C) in an oxygen-rich environment. The gas products were passed into the novel hollow electrode glow discharge ion source-quadrupole mass spectrometry. A standard curve of y = 64365x - 29953 ,R2=0.9997 was plotted by detecting the NO+ ion intensity, relative standard deviation (RSD) of 1.8% to 5.7%.Using the same method, food sample No. 6 and 7 were combusted and NO+ ion intensity was measured to verify the accuracy of the quantitation curve. Subsequently, the protein content was determined using a nitrogen-to-protein conversion factor of 6.25. This method provides a rapid, accurate, and environmentally friendly approach for determining protein content in food
食品中的蛋白质含量是衡量食品营养价值和安全性的重要指标,因此准确检测食品中的蛋白质含量具有重要意义。本文设计了燃烧炉和新型空心电极辉光放电离子源-四极杆质谱(HGD-MS)。利用它们构建了一个 "燃烧炉+质谱 "实验平台来检测食品中的蛋白质含量。选取五种食品标准样品进行分析。食品样品在燃烧炉中高温(1300°C)富氧环境下燃烧。气体产物进入新型空心电极辉光放电离子源-四极杆质谱仪。通过检测 NO+ 离子强度,绘制了 y = 64365x - 29953 的标准曲线,R2=0.9997,相对标准偏差(RSD)为 1.8%-5.7%。随后,使用氮-蛋白质转换系数 6.25 测定蛋白质含量。该方法为测定食品中的蛋白质含量提供了一种快速、准确和环保的方法。
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引用次数: 0
Removal and detection of phenols through an SPE-HPLC method using microporous organic networks as adsorbent. 使用微孔有机网络作为吸附剂,通过 SPE-HPLC 方法去除和检测苯酚。
IF 2.7 3区 化学 Q2 CHEMISTRY, ANALYTICAL Pub Date : 2024-07-12 DOI: 10.1039/d4ay00799a
Azam Ali, Huipeng Sun, Syed Faheem Askari Rizvi, Ding Nana, Haixia Zhang

The design and development of a facile synthesis approach to construct novel materials for the rapid adsorption and removal of environmental pollutants are of significant interest. In this work, we report the rational design and facile synthesis of magnetic core-shell-based microporous organic networks, Fe3O4@MON-TBPT-TEB (TTMON, achieved by reacting 2,4,6-tris(p-bromophenyl) triazine and 1,3,5-triethynylbenzene) and Fe3O4@MON-TBPM-DEBP (TDMON, achieved by reacting tetrakis (4-bromophenyl) methane and 4-4'-diethynylbiphenyl). These MONs possessed excellent dispersity, electrostatic attraction as well as plenty of π-π and hydrophobic interaction sites enabled them to efficiently absorb targeted environmental pollutants. TTMON and TDMON exhibited excellent adsorption capacities of 440 and 510 mg g-1, respectively, at 25 °C for 2,4,6-trichlorophenol (TCP). TCP, 2,4-dichlorophenol (DCP), 2-naphthol (2-NT) and 4-nitrophenol (4-NP) from aqueous solution were treated by both MONs, followed by their analysis with high-performance liquid chromatography (HPLC). For TDMON, the proposed SPE-HPLC-UV method showed an LOD of 0.03 μg L-1, LOQ of 0.11 μg L-1, and a wide linear range of 1-1000 μg L-1 for TCP. The adsorption kinetics, thermodynamics, isotherms, effect of pH and humic acid (HA), ionic strength, regeneration, and reusability of the MONs were also studied. The results revealed that the novel-designed MONs have potential applications as efficient adsorbents in sample pretreatment.

设计和开发一种简便的合成方法来构建快速吸附和去除环境污染物的新型材料具有重要意义。在这项工作中,我们报告了基于磁芯壳的微孔有机网络--Fe3O4@MON-TBPT-TEB(TTMON,由 2,4,6-三(对溴苯基)三嗪和 1,3,5-三乙炔基苯反应实现)和 Fe3O4@MON-TBPM-DEBP(TDMON,由四(4-溴苯基)甲烷和 4-4'-二乙炔基联苯反应实现)的合理设计和简便合成。这些 MONs 具有出色的分散性、静电吸引力以及大量的 π-π 和疏水相互作用位点,使它们能够有效地吸收目标环境污染物。25 °C 时,TTMON 和 TDMON 对 2,4,6-三氯苯酚(TCP)的吸附容量分别为 440 毫克/克和 510 毫克/克。用这两种 MON 处理水溶液中的 TCP、2,4-二氯苯酚(DCP)、2-萘酚(2-NT)和 4-硝基苯酚(4-NP),然后用高效液相色谱法(HPLC)进行分析。对于TDMON,所提出的SPE-HPLC-UV方法的LOD为0.03 μg L-1,LOQ为0.11 μg L-1,TCP的线性范围为1-1000 μg L-1。此外,还研究了 MONs 的吸附动力学、热力学、等温线、pH 值和腐植酸(HA)的影响、离子强度、再生和重复使用性。研究结果表明,设计新颖的 MONs 可作为高效吸附剂用于样品预处理。
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引用次数: 0
LAMP-CRISPR/Cas12b rapid detection platform for canine parvovirus detection 用于检测犬细小病毒的 LAMP-CRISPR/Cas12b 快速检测平台
IF 3.1 3区 化学 Q2 CHEMISTRY, ANALYTICAL Pub Date : 2024-07-12 DOI: 10.1039/d4ay00977k
Yuting Chen, Xinyu Zhang, Gui Hu, Yueying Pan, Yuhong Guan, Jinquan Yang, Hui Chen
Canine parvovirus (CPV) is one of the main pathogens causing toxic diarrhea in Chinese dogs, which is the cause of large-scale epidemic of dogs, and poses a great threat to the dog industry in China. Rapid, sensitive, and specific CPV testing facilitates the timely diagnosis and treatment of sick dogs. The aim of this study was to build a LAMP-CRISPR/Cas12b platform for CPV detection. The loop mediated isothermal amplification (LAMP) technique was combined with CRISPR-Cas12b analysis to establish a “two-step” and “one-tube” CRISPR/Cas12b rapid CPV method, respectively. The detection system was constructed with specific LAMP amplification primers and single guide RNA (sgRNA) for the highly conserved short fragment of CPV gene, which could be detected within 1h without cross-reaction with the other viruses causing canine diarrhea. The detection limits of both “two-step” and “one-tube” CRISPR/Cas12b reactions were 10-1 copies/μL, which was 100 times sensitive than qPCR and LAMP.In order to achieve point-of-care testing (POCT) of CPV, a one-tube LAMP-CRISPR/Cas12b nucleic acid extraction and detection platform based on magnetic nanoparticle enrichment technology was established to achieve "sample in-result out".The results of this method on simulated samples were compared with those of quantitative Real-time PCR,the results showed 100% consistency, and the time was shorter, which could detect the diseased dogs earlier and provide the basis for clinical diagnosis. The LAMP-CRISPR/Cas12b method established in this study provides a sensitive and specific method for rapid detection of CPV, and provides technical support for rapid diagnosis of CPV.
犬细小病毒(CPV)是引起我国犬只中毒性腹泻的主要病原体之一,是造成犬只大规模流行的原因,对我国养犬业构成极大威胁。快速、灵敏、特异的 CPV 检测有助于及时诊断和治疗病犬。本研究旨在建立一个用于CPV检测的LAMP-CRISPR/Cas12b平台。将环介导等温扩增(LAMP)技术与CRISPR-Cas12b分析相结合,分别建立了 "两步法 "和 "一管法 "CRISPR/Cas12b CPV快速检测方法。该检测系统由特异性LAMP扩增引物和针对CPV基因高度保守短片段的单导RNA(sgRNA)构建而成,可在1小时内完成检测,且不会与引起犬腹泻的其他病毒产生交叉反应。为了实现CPV的床旁检测(POCT),建立了基于磁性纳米粒子富集技术的一管式LAMP-CRISPR/Cas12b核酸提取和检测平台,实现了 "样品进,结果出"。该方法对模拟样本的检测结果与实时定量 PCR 检测结果进行了比较,结果一致性达到 100%,且检测时间更短,可更早地发现病犬,为临床诊断提供依据。本研究建立的LAMP-CRISPR/Cas12b方法为CPV的快速检测提供了灵敏、特异的方法,为CPV的快速诊断提供了技术支持。
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引用次数: 0
A novel flexible Magnetoelastic Biosensor Based on PDMS/FeSiB/QDs composite film for the Detection of African Swine Fever Virus P72 protein 基于 PDMS/FeSiB/QDs 复合薄膜的新型柔性磁弹性生物传感器用于检测非洲猪瘟病毒 P72 蛋白
IF 3.1 3区 化学 Q2 CHEMISTRY, ANALYTICAL Pub Date : 2024-07-12 DOI: 10.1039/d4ay01057d
Yuanhang Liu, Shengbo Sang, Dong Zhao, Yang Ge, Juanjuan Xue, Qianqian Duan, Xing Guo
African swine fever (ASF) is a highly contagious and severe hemorrhagic disease caused by the African swine fever virus (ASFV). The continuous spread of ASFV affects the safety of global meat supply, so the establishment of sensitive and specific detection methods for ASFV has become an important hot spot in food safety. Herein, we developed a flexible magnetoelastic (ME) biosensor based on PDMS/FeSiB/QDs composite films for the detection of ASFV. Based on the high luminescence performance of CsPbBr3 quantum dots and the excellent magnetoelastic effect of FeSiB, flexible ME biosensors convert stress signals generated by antibody-antigen-specific binding into optical and electromagnetic signals. The nanostructures covalently linked by quantum dots and PDMS provide biomodification sites for ASFV antibodies, simplifying the functionalization modification process compared to conventional biosensors. The deformation of the PDMS film is amplified and conversion of surface stress signal to electrical signal is enhanced by exposing the biosensor to a uniform magnetic field. The experimental results proved that the flexible ME biosensor has wide linear range of 10 ng/mL - 100 μg/mL, and the detection limit is as low as 0.7933 ng/mL. Moreover, the flexible ME biosensor have also shown to good stability, sensitivity and specificity, confirming the potential for early disease screening.
非洲猪瘟(ASF)是由非洲猪瘟病毒(ASFV)引起的一种传染性极强的严重出血性疾病。非洲猪瘟病毒的持续传播影响着全球肉类供应安全,因此建立灵敏、特异的非洲猪瘟病毒检测方法已成为食品安全领域的重要热点。在此,我们开发了一种基于 PDMS/FeSiB/QDs 复合薄膜的柔性磁弹性(ME)生物传感器,用于检测 ASFV。基于 CsPbBr3 量子点的高发光性能和 FeSiB 的优异磁弹性效应,柔性 ME 生物传感器可将抗体-抗原特异性结合产生的应力信号转化为光学和电磁信号。量子点和 PDMS 共价连接的纳米结构为 ASFV 抗体提供了生物修饰位点,与传统生物传感器相比,简化了功能化修饰过程。将生物传感器置于均匀磁场中,可放大 PDMS 薄膜的形变,并增强表面应力信号到电信号的转换。实验结果证明,柔性 ME 生物传感器具有 10 ng/mL - 100 μg/mL 的宽线性范围,检测限低至 0.7933 ng/mL。此外,柔性 ME 生物传感器还具有良好的稳定性、灵敏度和特异性,证实了其在疾病早期筛查方面的潜力。
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引用次数: 0
Chemically modified graphitic carbon nitride nanosheets for the selective turn-off fluorescence detection of Al(III) ions in crabs (Brachyura). 化学修饰石墨氮化碳纳米片用于螃蟹(Brachyura)体内铝(III)离子的选择性熄灭荧光检测。
IF 2.7 3区 化学 Q2 CHEMISTRY, ANALYTICAL Pub Date : 2024-07-12 DOI: 10.1039/d4ay00806e
Y G Abou El-Reash, Osama El-Awady, Faisal K Algethami, Fathi S Awad

The selective and sensitive detection of Al(III) is critically important for human health since the level of Al(III) is an indicator of many diseases in humans. Herein, we developed a simple and sensitive fluorescent sensor for the detection of Al(III) in an aqueous solution based on the fluorescence of hydroxyl-functionalized graphitic carbon nitride nanosheets (HO/g-CN). OH/g-CN nanosheets were synthesized via the thermal pyrolysis of 1,3,5-triazine-2,4,6-triamine (as raw material) at 550 °C for 2 hours, followed by thermal alkali treatment at 730 °C for 2 min. The fluorescence of HO/g-CN at 377 nm (at 290 nm excitation) can be quenched by Al(III) effectively and selectively, and the linear relationship between the concentration of Al(III) and fluorescence intensity is in the range of 1.85-14.82 μM with a detection limit of 0.272 μM. The fluorescence turn-off effect of the Al(III) ion on the prepared HO/g-CN nanosheets could be attributed to the presence of oxygen- and nitrogen-containing functional groups on the surface of HO/g-CN that have chelating interactions with Al(III), leading to quenching. The surface functional groups of OH/g-CN were confirmed using different characterization techniques (FTIR, EDX, and XPS). Moreover, the prepared HO/g-CN exhibited remarkable long-term fluorescence stability in water (>30 days) and minimal toxicity. Importantly, a prepared novel fluorescent sensor (HO/g-CN) was successfully applied for the detection and determination of Al(III) in commercially available crab (Brachyura) samples.

由于 Al(III)的含量是人类多种疾病的指标,因此选择性灵敏地检测 Al(III)对人类健康至关重要。在此,我们基于羟基功能化氮化石墨碳纳米片(HO/g-CN)的荧光,开发了一种简单灵敏的荧光传感器,用于检测水溶液中的铝(III)。羟基/g-CN 纳米片是以 1,3,5-三嗪-2,4,6-三胺(原料)为原料,在 550 °C 下热解 2 小时,然后在 730 °C 下碱热处理 2 分钟合成的。在 290 nm 激发下,HO/g-CN 在 377 nm 处的荧光可被 Al(III)有效地、选择性地淬灭,Al(III)浓度与荧光强度的线性关系范围为 1.85-14.82 μM,检测限为 0.272 μM。铝(III)离子对制备的 HO/g-CN 纳米片的荧光熄灭效应可能是由于 HO/g-CN 表面存在含氧和含氮的官能团,这些官能团与铝(III)发生螯合作用,从而导致荧光熄灭。利用不同的表征技术(傅立叶变换红外光谱、电离辐射X和 XPS)证实了 OH/g-CN 的表面官能团。此外,所制备的 HO/g-CN 在水中具有显著的长期荧光稳定性(大于 30 天),且毒性极低。重要的是,制备的新型荧光传感器(HO/g-CN)被成功应用于检测和测定市售螃蟹(Brachyura)样品中的铝(III)。
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引用次数: 0
Electrochemical Ratiometric Immunosensor for the Detection of NMP22 Based on ZIF-8@MWCNTs@Chit@Fc@AuNPs and AuPt-MB 基于 ZIF-8@MWCNTs@Chit@Fc@AuNPs 和 AuPt-MB 的检测 NMP22 的电化学比率计量免疫传感器
IF 3.1 3区 化学 Q2 CHEMISTRY, ANALYTICAL Pub Date : 2024-07-11 DOI: 10.1039/d4ay01066c
Zhiren zhou, Yuting Tian, Lina Zou, Yanan Liu, Xueqing Zhang, Xiaojing Huang, Huanyu Ren, Zheng Li, Huiru Niu, Hao Liao, Xiaojing Zhang, Hongzhi Pan, Shengzhong Rong, Hongkun Ma
Nuclear matrix protein 22 (NMP22) is one of the most important tumor marker of bladder cancer, which is significantly elevated in the urine of bladder cancer patients. Therefore, in this work, a highly sensitive ratiometric electrochemical immunosensor was constructed to detect NMP22 based on ZIF-8@MWCNTs@Chit@Fc@AuNPs composite. ZIF-8 had a large surface area and good adsorption ability. Multi-Walled Carbon Nanotubes (MWCNTs) can optimize the electrical conductivity of ZIF-8, so that the electrode surface of ferrocene (Fc) obtains a stable and strong electrochemical signal. In addition, AuPt-MB provided another strong detection signal methylene blue (MB) while immobilizing the secondary antibody (Ab2) through Au-N and Pt-N bonds. A ratiometric electrochemical sensor was formed based on ZIF-8@MWCNTs@Chit@Fc@AuNPs and AuPt-MB, which showed a great linear connection between IMB/IFc and the logarithmic concentration of NMP22 with a detection limit of 3.33 fg mL-1 (S/N=3) under optimized specifications in the concentration interval of 0.01 pg mL-1-1000 ng mL-1. In addition, the ratiometric immunosensor showed good selectivity and stability.
核基质蛋白22(NMP22)是膀胱癌最重要的肿瘤标志物之一,在膀胱癌患者尿液中明显升高。因此,本研究基于 ZIF-8@MWCNTs@Chit@Fc@AuNPs 复合材料构建了一种高灵敏度的比率电化学免疫传感器来检测 NMP22。ZIF-8具有较大的比表面积和良好的吸附能力。多壁碳纳米管(MWCNTs)可以优化 ZIF-8 的导电性,从而使二茂铁(Fc)电极表面获得稳定而强烈的电化学信号。此外,AuPt-MB 在通过 Au-N 和 Pt-N 键固定二抗(Ab2)的同时,还提供了另一种强检测信号亚甲基蓝(MB)。基于 ZIF-8@MWCNTs@Chit@Fc@AuNPs 和 AuPt-MB 制成的比率测量电化学传感器在 0.01 pg mL-1-1000 ng mL-1 的浓度范围内显示出 IMB/IFc 与 NMP22 对数浓度之间的良好线性关系,在优化规格下的检测限为 3.33 fg mL-1 (S/N=3)。此外,该比率计量免疫传感器还具有良好的选择性和稳定性。
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引用次数: 0
Development and Validation of RP-HPLC Method for Quantification of Tavaborole 用于塔伐伯乐定量的 RP-HPLC 方法的开发与验证
IF 3.1 3区 化学 Q2 CHEMISTRY, ANALYTICAL Pub Date : 2024-07-11 DOI: 10.1039/d4ay00943f
SHIV KUMAR Kumar PRAJAPATI, Ankit Jain, Meenakshi Bajpai
The stability-indicating approach for tavaborole quantification was developed and validated to establish a precise, linear, accurate, and robust HPLC method. The development section includes optimizing the detection wavelength, the mobile phase ratio, and the type of column used to achieve the best possible separation and sensitivity for analysis. The chromatographic conditions were established, considering peak symmetry, resolution, and retention time. The mobile phase composition, comprising a buffer: acetonitrile (75:25, %v/v), with an injection volume of 15 µL, showed suitable elution and recovery at 265 nm. A constant column oven temperature of 35°C and a 1 mL/min flow rate were maintained. The pH of the buffer was changed to 3.0 by using orthophosphoric acid. Linearity was observed from 5 to 1000 ppm (r2=1.00000). The capacity (retention) factor (k) of 3.43 was observed, indicating significant interaction and good separation. Forced degradation (FD) or stress tests were performed for chemical and physical photolytic stress conditions, and the results observed were within the specified limits. The stability in the analytical solution was observed for up to 35 hours at 5 °C, confirming the stability of the solution. Validation of the developed HPLC method confirmed the system’s suitability, precision, linearity, accuracy, FD, robustness, and results. All validation criteria for the technique were within acceptable limits.
为建立精确、线性、准确和稳健的高效液相色谱法,我们开发并验证了他伐伯乐定量的稳定性指示方法。开发过程包括优化检测波长、流动相比例和色谱柱类型,以达到最佳的分离度和分析灵敏度。考虑到峰值对称性、分辨率和保留时间,确定了色谱条件。流动相组成为缓冲液:乙腈(75:25,%v/v),进样量为 15 µL,在 265 纳米波长下显示出合适的洗脱和回收率。柱温恒定在 35°C,流速为 1 mL/min。使用正磷酸将缓冲液的 pH 值调至 3.0。线性范围为 5 至 1000 ppm(r2=1.00000)。观察到容量(保留)因子(k)为 3.43,表明相互作用显著,分离效果良好。在化学和物理光解应力条件下进行了强制降解(FD)或应力测试,结果均在规定范围内。在 5 °C 下,分析溶液的稳定性可长达 35 小时,这证实了溶液的稳定性。对所开发的高效液相色谱法的验证证实了该系统的适用性、精密度、线性度、准确度、FD、稳健性和结果。该技术的所有验证标准均在可接受范围内。
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引用次数: 0
Quality and efficiency assessment of five extracellular vesicle isolation methods using resistive pulse sensing strategy 利用电阻脉冲传感策略评估五种细胞外囊泡分离方法的质量和效率
IF 3.1 3区 化学 Q2 CHEMISTRY, ANALYTICAL Pub Date : 2024-07-10 DOI: 10.1039/d4ay01158a
Min Yang, Jia Guo, Ze Chen, Ying Liu, Zepeng Sun, Xin Pang, Yinghua Peng
Extracellular vesicles (EVs) have attracted great interest due to their great potential in disease diagnosis and therapy. The separation of EVs from complex biofluids with high purity is essential for the accurate analysis of EVs. Despite various methods, there is still no consensus on the best method for high-quality EVs isolation and reliable mass production. Therefore, it is important to offer a standardized method for characterizing the properties (size distribution, particle concentration and purity) of EV preparations from different isolation methods. Herein, we employed a NanoCoulter Counter based on resistive pulse sensing (RPS) strategy that enabled multi-parameter analysis of single EV to compare the quality and efficiency of different EV isolation techniques including traditional differential ultracentrifugation, ultrafiltration, size exclusion chromatography, membrane affinity binding and polymer precipitation. The data revealed that the NanoCoulter Counter based on RPS strategy was reliable and effective for the characterization of EVs. The results suggested that although higher particle concentrations were observed in three commercial isolation kits and ultrafiltration, traditional differential ultracentrifugation possessed the highest purity. In conclusion, our results from NanoCoulter Counter provided reliable evidence for the assessment of different EVs isolation method, which contributed to the development of EV-based disease biomarkers and treatments.
由于细胞外囊泡(EVs)在疾病诊断和治疗方面具有巨大潜力,因此引起了人们的极大兴趣。从复杂的生物流体中分离出高纯度的 EVs 对准确分析 EVs 至关重要。尽管方法多种多样,但对于高质量 EVs 分离和可靠的大规模生产的最佳方法仍未达成共识。因此,提供一种标准化的方法来表征来自不同分离方法的 EV 制剂的特性(大小分布、颗粒浓度和纯度)非常重要。在此,我们采用了基于电阻脉冲传感(RPS)策略的 NanoCoulter 计数器,该计数器可对单个 EV 进行多参数分析,以比较不同 EV 分离技术(包括传统的差分超速离心、超滤、尺寸排阻色谱、膜亲和结合和聚合物沉淀)的质量和效率。数据显示,基于 RPS 策略的 NanoCoulter 计数器能可靠、有效地表征 EVs。结果表明,虽然在三种商业分离试剂盒和超滤中观察到的颗粒浓度较高,但传统的差分超速离心法拥有最高的纯度。总之,NanoCoulter 计数器的结果为评估不同的 EVs 分离方法提供了可靠的证据,有助于开发基于 EVs 的疾病生物标记物和治疗方法。
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引用次数: 0
期刊
Analytical Methods
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