Natural Product Reports最新文献

Covering: 2020 to 2022This review described the total synthesis of naturally occurring cyclic peptides with unique structures covering 2020 to 2022, i.e., darobactin A, pyritide A2, decatransin, mannopeptimycin β, α- and β-amanitins, orfamide A, and MA026, paying particular attention to the construction of their unique structures via macrocyclization.

Jasmonates, including jasmonic acid (JA) and its derivatives, are lipid-based signaling molecules critical for plant growth, development, and defense. Among these, jasmonoyl-L-isoleucine (JA-Ile) has been identified as a bioactive plant hormone that mediates various physiological responses. JA-Ile functions in planta as a 'molecular glue' in protein-protein associations to induce the defense-related gene expression for plant-pathogen and plant-insect communications, and it affects many aspects of plant development and stress responses. This review explores the historical journey of jasmonate research, emphasizing the discovery of JA-Ile, its biosynthesis, function as a molecular glue, and the ligand-receptor co-evolutional aspect. The elucidation of the SCFCOI1-JAZ receptor complex and the crystallization of this co-receptor system marked significant advancements in understanding the chemical background of jasmonate biology. This review focuses on the advances in the chemistry and biology of jasmonate bioscience in the past two decades.

Covering: 2019 to early 2025Marine sponges form unique ecosystems through symbiosis with diverse microbial communities, producing natural products including bioactive compounds. This review comprehensively addresses the key steps in the discovery of natural products from sponge-associated microorganisms, encompassing microbial isolation and cultivation, compound identification, and characterisation. Various cultivation methods, such as floating filter cultivation, microcapsule-based cultivation, and in situ systems, are examined to highlight their applications and strategies for overcoming limitations of conventional approaches. Additionally, the integration of genome-based methodologies and compound screening is explored to enhance the discovery of novel bioactive substances and establish a sustainable platform for natural product research. This review provides insights into the latest trends in sponge-associated microbial research and offers practical perspectives for expanding the utilization of marine biological resources.

Covering: 1992 to 2024Type-I polyketide synthase (PKS)-derived metabolites are structurally diverse bioactive natural products containing multiple stereogenic centres. This review focuses on the configurational analysis of type-I PKS-derived natural products, emphasizing the methodologies and challenges associated with determining their stereochemistry due to their complex structures with multiple chiral centres. Key strategies include J-based configuration analysis (JBCA), chemical derivatizations with chiral reagents, degradation methods, NMR spectroscopic analysis, and the exploitation of chiroptical properties. Case studies demonstrate the practical applications of these methods in elucidating the stereochemistry of type-I polyketide natural products.

Covering: 2014 up to February 2025Since the Nagoya Protocol came into force in 2014, scientists working with genetic resources have integrated compliance with Access and Benefit-Sharing (ABS) legislation at international and national levels into their research practices. However, two key gaps left by the Nagoya Protocol are being addressed, introducing new obligations for marine natural product scientists: under the auspices of the Convention on Biological Diversity (CBD), a compromise agreement was reached in November 2024 that regulates the use of Digital Sequence Information (DSI) on Genetic Resources. Within the next few years, the 2023 Biodiversity Beyond National Jurisdiction (BBNJ) Agreement is expected to take effect. This treaty covers the access to and use of marine biodiversity of areas beyond national jurisdiction for research and development. In a time when genetic research and marine biodiversity are key to scientific advancement, these evolving policies affect how genetic information is stored, shared, and used, raising emerging questions for the scientific community about their direct impact and the complexities of compliance. Despite continuous developments and scientific community involvement, there remains a notable gap in communication between policy changes and their accessible dissemination to researchers. Addressing this gap is crucial for the continuation of research and the effective use of relevant resources. The main goal of this viewpoint article is to provide a concise guide to recent policy developments relevant to natural product researchers that should be incorporated and harmonized into ongoing scientific activities.

Covering: 2014 to 2024Cyanobacteria are prolific producers of bioactive natural products, including promising drug leads for FDA-approved cancer therapeutics. Advances in genome sequencing and computational tools have revealed a wealth of cyanobacterial biosynthetic gene clusters (BGCs). However, progress in genome-driven discovery has been hindered by challenges in manipulating native hosts and the limited availability of efficient heterologous expression platforms. This highlight focuses on recent synthetic biology innovations on cyanobacterial systems that address these obstacles, facilitating the production of diverse cyanobacterial natural product families. We discuss key features of widely used cyanobacterial chassis, such as Synechocystis sp. PCC 6803, Synechococcus elongatus UTEX 2973, Anabaena sp. PCC 7120, and emerging hosts. Advances in BGC cloning, combinatorial biosynthesis, transcriptional and translational regulation, and host engineering are also highlighted. Together, these synthetic biology developments provide a powerful framework for expanding cyanobacterial natural product discovery and production.

Covering: up to 2024The ubiquitin-proteasome system (UPS) plays a key role in regulating intracellular protein degradation and maintaining cellular homeostasis. Within the UPS, target proteins are polyubiquitinated through sequential reactions catalyzed by ubiquitination-related enzymes. These ubiquitinated proteins are then recognized and degraded by the 26S proteasome. Deubiquitinating enzymes cleave the formed polyubiquitin chains and regulate protein degradation, thereby contributing to precise regulation of the system. Dysregulation of the UPS is associated with cancer, immune disorders, and neurodegenerative diseases, making it a potential target for drug discovery. To date, a variety of natural products that target the UPS have been discovered and used in pharmaceutical development, and these compounds have provided important insights into the molecular mechanisms of UPS regulation. This review describes natural products that inhibit protein degradation in the UPS and activate protein degradation mediated by the 20S proteasome, thus clarifying their mechanisms of action and exploring their potential applications as therapeutic agents.

Covering: up to February 2025Among the numerous bioactive microbial natural products, a subset of nonribosomal peptides derived from actinobacteria is characterized by their C₂-symmetric macrocyclic scaffolds and referred to as bisintercalators due to their ability to bisintercalate into DNA molecules. This family of compounds exhibits excellent antimicrobial, antitumor and antiviral properties, making them promising candidates for drug development. New members of the bisintercalator family continue to be discovered, and significant advancement has been made in understanding their biosynthesis over the past two decades. These efforts have established the general biosynthetic pathways of bisintercalators, although some chemically intriguing enzymatic transformations remain to be fully elucidated. This review summarizes the sources and chemical structures of known bisintercalators, briefly discussing their bioactivities, and then highlights the biochemical reactions involved in assembling their sophisticated macrocyclic scaffolds.

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Covering: 2000 to 2025This review explores the potential of artificial intelligence (AI) in addressing challenges and accelerating molecular insights in biosynthetic pathway research, which is crucial for developing bioactive natural products with applications in pharmacology, agriculture, and biotechnology. It provides an overview of various AI techniques relevant to this research field, including machine learning (ML), deep learning (DL), natural language processing, network analysis, and data mining. AI-powered applications across three main areas, namely, pathway discovery and mining, pathway design, and pathway optimization, are discussed, and the benefits and challenges of integrating omics data and AI for enhanced pathway research are also elucidated. This review also addresses the current limitations, future directions, and the importance of synergy between AI and experimental approaches in unlocking rapid advancements in biosynthetic pathway research. The review concludes with an evaluation of AI's current capabilities and future outlook, emphasizing the transformative impact of AI on biosynthetic pathway research and the potential for new opportunities in the discovery and optimization of bioactive natural products.