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Advancing Bioactive Material for Mandibular Bone Regeneration: Transformation of Fibrous Mat into 3D Matrix Cotton for Enhanced Shape Retention and Rapid Hemostasis. 促进下颌骨再生的生物活性材料:将纤维垫转化为三维基质棉,以增强形状保持力和快速止血。
IF 5.4 2区 医学 Q2 MATERIALS SCIENCE, BIOMATERIALS Pub Date : 2024-07-18 DOI: 10.1021/acsbiomaterials.4c01148
Shalini Thomas, Ajay Rakkesh Rajendran, Bargavi Purushothaman, Balakumar Subramanian

Transformation of a fibrous mat into a three-dimensional (3D) scaffold opens up abundant innovative prospects in biomedical research, particularly for studying both soft as well as hard tissues. Electrospun nanofibers, which mimic the extracellular matrix have attracted significant attention in various studies. This research focuses on rapidly converting a fibrous mat made of polycaprolactone (PCL)/pluronic F-127 (PF-127) with different percentages of monetite calcium phosphate (MCP) into desirable 3D matrix cotton using a unique gas foaming technology. These matrix cottons possess biomimetic properties and have oriented porous structures. Using this innovative technique, various shapes of 3D matrix cotton, such as squares, hollow tubes, and other customizable forms, were successfully produced. Importantly, these 3D matrix cottons showed a consistent distribution of monetite particles with total porosity ranging from 90% to 98%. The structure of the 3D matrix cotton, its water/blood absorption capacity, the potential for causing non-hemolysis, and rapid hemostatic properties were thoroughly investigated. Additionally, periodontal cells were cultured on the 3D matrix cotton to assess their viability and morphology, revealing promising results. Furthermore, a coculture study involving NIH-3T3 and MG-63 cells on the 3D matrix cotton showed spheroidal formation within 24 h. Notably, in vitro assessments indicated that the matrix cotton containing 15% monetite (PCL-MMC15%) exhibited superior absorbent capabilities, excellent cell viability, and rapid hemostatic characteristics. Subsequently, the effectiveness of PCL-MMC15% in promoting mandibular bone regeneration was evaluated through an in vivo study on rabbits using a mandibular injury model. The results demonstrated that PCL-MMC15% facilitated the resolution of defects in the mandibular region by initiating new bone formation. Therefore, the presented 3D matrix cotton (PCL-MMC15%) shows significant promise for applications in both mandibular bone regeneration and hemostasis.

将纤维垫转化为三维(3D)支架为生物医学研究开辟了丰富的创新前景,尤其是在研究软组织和硬组织方面。模仿细胞外基质的电纺纳米纤维在各种研究中都引起了极大的关注。这项研究的重点是利用一种独特的气体发泡技术,将由聚己内酯(PCL)/pluronic F-127 (PF-127)和不同比例的莫来石磷酸钙(MCP)制成的纤维垫快速转化为理想的三维基质棉。这些基质棉具有仿生物特性和定向多孔结构。利用这种创新技术,成功生产出了各种形状的三维基质棉,如正方形、空心管和其他可定制的形状。重要的是,这些三维基质棉显示出一致的莫尼石颗粒分布,总孔隙率从90%到98%不等。对三维基质棉的结构、吸水/吸血能力、不溶血的潜力以及快速止血特性进行了深入研究。此外,还在三维基质棉上培养了牙周细胞,以评估其存活率和形态,结果令人欣喜。此外,在三维基质棉上进行的 NIH-3T3 和 MG-63 细胞共培养研究显示,在 24 小时内,细胞形成了球形。体外评估结果表明,含有 15% 莫尼石的基质棉(PCL-MMC15%)具有卓越的吸收能力、出色的细胞存活率和快速止血特性。随后,通过使用下颌骨损伤模型对兔子进行体内研究,评估了 PCL-MMC15% 在促进下颌骨再生方面的有效性。结果表明,PCL-MMC15% 通过启动新骨形成,促进了下颌骨区域缺损的愈合。因此,这种三维基质棉(PCL-MMC15%)在下颌骨再生和止血方面的应用前景十分广阔。
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引用次数: 0
Multifunctional Nanosystem Based on Ultrasmall Carbon Dots for the Treatment of Acute Kidney Injury. 基于超小碳点的多功能纳米系统用于治疗急性肾损伤
IF 5.4 2区 医学 Q2 MATERIALS SCIENCE, BIOMATERIALS Pub Date : 2024-07-18 DOI: 10.1021/acsbiomaterials.4c00616
Hanchun Yao, Zhihui Zhu, Mengyu Liu, Fangfang Sun, Mengyu Du, Yilin Sun, Bin Du

Acute kidney injury (AKI) is a critical medical condition characterized by high morbidity and mortality rates. The pathogenesis of AKI potentially involves bursts of reactive oxygen species (ROS) bursts and elevated levels of inflammatory mediators. Developing nanoparticles (NPs) that downregulate ROS and inflammatory mediators is a promising approach to treat AKI. However, such NPs would be affected by the glomerular filtration barrier (GFB). Typically, NPs are too large to penetrate the glomerular system and reach the renal tubules─the primary site of AKI injury. Herein, we report the development of ultrasmall carbon dots-gallic acid (CDs-GA) NPs (∼5 nm). These NPs exhibited outstanding biocompatibility and were shown not only to efficiently eliminate ROS and alleviate oxidative stress but also to suppress the activation of the NF-κB signaling pathway, leading to a reduction in the release of inflammatory factors. Importantly, CDs-GA NPs were shown to be able to rapidly accumulate rapidly in the renal tissues without the need for intricate targeting strategies. In vivo studies demonstrated that CDs-GA NPs significantly reduced the incidence of cisplatin (CDDP)-induced AKI in mice, surpassing the efficacy of the small molecular drug, N-acetylcysteine. This research provides an innovative strategy for the treatment of AKI.

急性肾损伤(AKI)是一种以高发病率和高死亡率为特征的危重病症。急性肾损伤的发病机制可能涉及活性氧(ROS)爆发和炎症介质水平升高。开发能下调 ROS 和炎症介质的纳米粒子(NPs)是治疗急性肾损伤的一种很有前景的方法。然而,这种 NPs 会受到肾小球滤过屏障(GFB)的影响。通常情况下,NPs 体积太大,无法穿透肾小球系统到达肾小管--AKI 的主要损伤部位。在此,我们报告了超小碳点-金属酸(CDs-GA)NPs(∼5 nm)的开发情况。这些 NPs 具有出色的生物相容性,不仅能有效消除 ROS 和缓解氧化应激,还能抑制 NF-κB 信号通路的激活,从而减少炎症因子的释放。重要的是,CDs-GA NPs 无需复杂的靶向策略就能在肾脏组织中迅速积累。体内研究表明,CDs-GA NPs 能显著降低顺铂(CDDP)诱导的小鼠 AKI 的发生率,其疗效超过了小分子药物 N-乙酰半胱氨酸。这项研究为治疗 AKI 提供了一种创新策略。
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引用次数: 0
Enhanced Stability of α-Mangostin-Rich Extract and Selective Cytotoxicity against Cancer Cells via Encapsulation in Antioxidant Nanoparticles (AME@NanoAOX). 通过封装在抗氧化剂纳米颗粒(AME@NanoAOX)中增强富含α-芒果苷的提取物的稳定性和对癌细胞的选择性细胞毒性。
IF 5.4 2区 医学 Q2 MATERIALS SCIENCE, BIOMATERIALS Pub Date : 2024-07-18 DOI: 10.1021/acsbiomaterials.4c00997
Wiwit Suttithumsatid, Takuto Toriumi, Wanida Sukketsiri, Yukio Nagasaki, Pharkphoom Panichayupakaranant

α-Mangostin-rich extract (AME) shows promise as a functional ingredient for cancer chemotherapy. Here, we encapsulated AME in our originally designed antioxidant nanoparticles (NanoAOX) to increase its solubility and prevent oxidative degradation (AME@NanoAOX). In this study, two types of self-assembled polymers containing nitroxide radicals were engineered. These polymers were self-assembled into nanoscale particles in aqueous media, entrapping AME (abbreviated as AME@NanoAOX(B) and AME@NanoAOX(G)). These formulations considerably improved the stability of AME against oxidative degradation and exhibited different release profiles of α-mangostin under different pH conditions. Furthermore, AME-encapsulated nanoparticles exhibited potent cytotoxicity against various cancer cell lines, including human breast cancer (MCF-7), human lung cancer (A549), human colon cancer (Caco-2), human cervical cancer (HeLa), and human liver cancer (HepG2) cell lines, with minimal cytotoxicity in normal human mammary epithelial cells (hTERT-HME1), thus providing a high selectivity index (SI). These results indicated the promising feature of AME-encapsulated antioxidant nanoparticles (AME@NanoAOX) for cancer chemotherapy.

富含α-芒果苷的提取物(AME)有望成为癌症化疗的功能性成分。在这里,我们将 AME 封装在我们最初设计的抗氧化纳米粒子(NanoAOX)中,以增加其溶解度并防止氧化降解(AME@NanoAOX)。本研究设计了两种含有亚硝基自由基的自组装聚合物。这些聚合物在水介质中自组装成纳米级颗粒,夹带 AME(缩写为 AME@NanoAOX(B) 和 AME@NanoAOX(G))。这些制剂大大提高了 AME 抗氧化降解的稳定性,并在不同的 pH 值条件下显示出不同的 α - 芒果苷释放曲线。此外,AME包囊纳米颗粒对多种癌细胞株,包括人乳腺癌(MCF-7)、人肺癌(A549)、人结肠癌(Caco-2)、人宫颈癌(HeLa)和人肝癌(HepG2)细胞株,都表现出了很强的细胞毒性,而对正常人乳腺上皮细胞(hTERT-HME1)的细胞毒性则很小,因此具有很高的选择性指数(SI)。这些结果表明,AME-封装抗氧化剂纳米粒子(AME@NanoAOX)在癌症化疗中具有广阔的应用前景。
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引用次数: 0
Multifunctional Glycopeptide-Based Hydrogel via Dual-Modulation for the Prevention and Repair of Radiation-Induced Skin Injury. 基于糖肽的多功能水凝胶通过双重调制预防和修复辐射引起的皮肤损伤
IF 5.4 2区 医学 Q2 MATERIALS SCIENCE, BIOMATERIALS Pub Date : 2024-07-17 DOI: 10.1021/acsbiomaterials.4c00698
Jiajun Guo, Xiaoguang Zhang, Ruiqi Mao, Hui Li, Yusen Hao, Jiamin Zhang, Wei Wang, Yumin Zhang, Jianfeng Liu

The radiation-induced skin injury (RISI) remains a great challenge for clinical wound management and care after radiotherapy, as patients will suffer from the acute radiation injury and long-term chronic inflammatory damage during the treatment. The excessive ROS in the early acute stage and prolonged inflammatory response in the late healing process always hinder therapeutic efficiency. Herein, we developed an extracellular matrix (ECM)-mimetic multifunctional glycopeptide hydrogel (oCP@As) to promote and accelerate RISI repair via a dual-modulation strategy in different healing stages. The oCP@As hydrogel not only can form an ECM-like nanofiber structure through the Schiff base reaction but also exhibits ROS scavenging and DNA double-strand break repair abilities, which can effectively reduce the acute radiation damage. Meanwhile, the introduction of oxidized chondroitin sulfate, which is the ECM polysaccharide-like component, enables regulation of the inflammatory response by adsorption of inflammatory factors, accelerating the repair of chronic inflammatory injury. The animal experiments demonstrated that oCP@As can significantly weaken RISI symptoms, promote epidermal tissue regeneration and angiogenesis, and reduce pro-inflammatory cytokine expression. Therefore, this multifunctional glycopeptide hydrogel dressing can effectively attenuate RISI symptoms and promote RISI healing, showing great potential for clinical applications in radiotherapy protection and repair.

放射治疗后,患者会遭受急性放射损伤和长期慢性炎症损伤,因此放射诱导的皮肤损伤(RISI)仍是临床伤口管理和护理的一大挑战。早期急性阶段过量的 ROS 和后期愈合过程中长时间的炎症反应始终阻碍着治疗的效率。在此,我们开发了一种仿细胞外基质(ECM)多功能糖肽水凝胶(oCP@As),通过双重调节策略在不同愈合阶段促进和加速 RISI 修复。oCP@As 水凝胶不仅能通过希夫碱反应形成类似 ECM 的纳米纤维结构,还具有清除 ROS 和 DNA 双链断裂修复能力,能有效减轻急性辐射损伤。同时,氧化硫酸软骨素作为 ECM 多糖类成分的引入,可通过吸附炎症因子调节炎症反应,加速慢性炎症损伤的修复。动物实验证明,oCP@As 能明显减轻 RISI 症状,促进表皮组织再生和血管生成,减少促炎细胞因子的表达。因此,这种多功能糖肽水凝胶敷料能有效减轻 RISI 症状,促进 RISI 愈合,在放疗保护和修复方面具有巨大的临床应用潜力。
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引用次数: 0
Microstructural, Fluid Dynamic, and Mechanical Characterization of Zinc Oxide and Magnesium Chloride-Modified Hydrogel Scaffolds. 氧化锌和氯化镁改性水凝胶支架的微结构、流体动力学和机械特性分析
IF 5.4 2区 医学 Q2 MATERIALS SCIENCE, BIOMATERIALS Pub Date : 2024-07-16 DOI: 10.1021/acsbiomaterials.4c00286
Murilo Daniel de Mello Innocentini, Bruno Ribeiro Fuzatto Bueno, Agnieszka Urbaś, Anna Morawska-Chochół

Scaffolds for the filling and regeneration of osteochondral defects are a current challenge in the biomaterials field, and solutions with greater functionality are still being sought. The novel approach of this work was to obtain scaffolds with biologically active additives possessing microstructural, permeability, and mechanical properties, mimicking the complexity of natural cartilage. Four types of scaffolds with a gelatin/alginate matrix modified with hydroxyapatite were obtained, and the relationship between the modifiers and substrate properties was evaluated. They differed in the type of second modifier used, which was hydrated MgCl2 in two proportions, ZnO, and nanohydroxyapatite. The samples were obtained by freeze-drying by using two-stage freezing. Based on microstructural observations combined with X-ray microanalysis, the microstructure of the samples and the elemental content were assessed. Permeability and mechanical tests were also performed. The scaffolds exhibited a network of interconnected pores and complex microarchitecture, with lower porosity at the surface (15 ± 7 to 29 ± 6%) and higher porosity at the center (67 ± 8 to 75 ± 8%). The additives had varying effects on the pore sizes and permeabilities of the samples. ZnO yielded the most permeable scaffolds (5.92 × 10-11 m2), whereas nanohydroxyapatite yielded the scaffold with the lowest permeability (1.18 × 10-11 m2), values within the range reported for trabecular bone. The magnesium content had no statistically significant effect on the permeability. The best mechanical parameters were obtained for ZnO samples and those containing hydrated MgCl2. The scaffold's properties meet the criteria for filling osteochondral defects. The developed scaffolds follow a biomimetic approach in terms of hierarchical microarchitecture and mechanical parameters as well as chemical composition. The obtained composite materials have the potential as biomimetic scaffolds for the regeneration of osteochondral defects.

用于填充和再生骨软骨缺损的支架是生物材料领域目前面临的一项挑战,人们仍在寻求具有更多功能的解决方案。这项工作的新方法是获得含有生物活性添加剂的支架,使其具有微观结构、渗透性和机械性能,模拟天然软骨的复杂性。研究人员获得了四种用羟基磷灰石修饰明胶/海藻酸盐基质的支架,并评估了修饰剂与基质特性之间的关系。它们使用的第二种改性剂类型不同,分别是两种比例的水合氯化镁、氧化锌和纳米羟基磷灰石。样品通过两级冷冻干燥获得。根据微观结构观察和 X 射线显微分析,对样品的微观结构和元素含量进行了评估。此外,还进行了渗透性和力学测试。支架呈现出相互连接的孔隙网络和复杂的微结构,表面孔隙率较低(15 ± 7% 到 29 ± 6%),中心孔隙率较高(67 ± 8% 到 75 ± 8%)。添加剂对样品的孔隙大小和渗透率有不同的影响。氧化锌产生的支架透气性最高(5.92 × 10-11 m2),而纳米羟基磷灰石产生的支架透气性最低(1.18 × 10-11 m2),这些数值都在报告的骨小梁范围内。镁含量对渗透性没有明显的统计学影响。氧化锌样品和含有水合氯化镁的样品获得了最佳力学参数。该支架的特性符合填充骨软骨缺损的标准。所开发的支架在分层微结构和机械参数以及化学成分方面采用了仿生方法。所获得的复合材料具有作为仿生物支架用于骨软骨缺损再生的潜力。
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引用次数: 0
NIR-II-Responsive Versatile Nanozyme Based on H2O2 Cycling and Disrupting Cellular Redox Homeostasis for Enhanced Synergistic Cancer Therapy. 基于 H2O2 循环和破坏细胞氧化还原平衡的近红外-II 反应性多功能纳米酶,用于增强癌症协同治疗。
IF 5.4 2区 医学 Q2 MATERIALS SCIENCE, BIOMATERIALS Pub Date : 2024-07-16 DOI: 10.1021/acsbiomaterials.4c00929
Pinghua Ling, Danjie Song, Pei Yang, Chuanye Tang, Wenwen Xu, Fang Wang

Disturbing cellular redox homeostasis within malignant cells, particularly improving reactive oxygen species (ROS), is one of the effective strategies for cancer therapy. The ROS generation based on nanozymes presents a promising strategy for cancer treatment. However, the therapeutic efficacy is limited due to the insufficient catalytic activity of nanozymes or their high dependence on hydrogen peroxide (H2O2) or oxygen. Herein, we reported a nanozyme (CSA) based on well-defined CuSe hollow nanocubes (CS) uniformly covered with Ag nanoparticles (AgNPs) to disturb cellular redox homeostasis and catalyze a cascade of intracellular biochemical reactions to produce ROS for the synergistic therapy of breast cancer. In this system, CSA could interact with the thioredoxin reductase (TrxR) and deplete the tumor microenvironment-activated glutathione (GSH), disrupting the cellular antioxidant defense system and augmenting ROS generation. Besides, CSA possessed high peroxidase-mimicking activity toward H2O2, leading to the generation of various ROS including hydroxyl radical (OH), superoxide radicals (O2-), and singlet oxygen (1O2), facilitated by the Cu(II)/Cu(I) redox and H2O2 cycling, and plentiful catalytically active metal sites. Additionally, due to the absorption and charge separation performance of AgNPs, the CSA exhibited excellent photothermal performance in the second near-infrared (NIR-II, 1064 nm) region and enhanced the photocatalytic ROS level in cancer cells. Owing to the inhibition of TrxR activity, GSH depletion, high peroxidase-mimicking activity of CSA, and abundant ROS generation, CSA displays remarkable and specific inhibition of tumor growth.

扰乱恶性细胞内的细胞氧化还原平衡,特别是改善活性氧(ROS),是癌症治疗的有效策略之一。基于纳米酶的 ROS 生成是一种很有前景的癌症治疗策略。然而,由于纳米酶的催化活性不足或对过氧化氢(H2O2)或氧气的高度依赖,其治疗效果受到限制。在此,我们报道了一种纳米酶(CSA),它基于均匀覆盖着Ag纳米颗粒(AgNPs)的定义明确的CuSe空心纳米立方体(CS),能扰乱细胞氧化还原平衡,催化一连串细胞内生化反应产生ROS,从而协同治疗乳腺癌。在该系统中,CSA 可与硫氧还原酶(TrxR)相互作用,消耗肿瘤微环境激活的谷胱甘肽(GSH),从而破坏细胞抗氧化防御系统并增加 ROS 的生成。此外,CSA 对 H2O2 具有很高的过氧化物酶模拟活性,在 Cu(II)/Cu(I) 氧化还原和 H2O2 循环以及丰富的催化活性金属位点的作用下,可生成包括羟自由基(-OH)、超氧自由基(-O2-)和单线态氧(1O2)在内的各种 ROS。此外,由于 AgNPs 的吸收和电荷分离性能,CSA 在第二近红外(NIR-II,1064 纳米)区域表现出优异的光热性能,提高了光催化癌细胞中 ROS 的水平。由于 CSA 对 TrxR 活性的抑制、GSH 的耗竭、高过氧化物酶模拟活性以及丰富的 ROS 生成,CSA 对肿瘤生长具有显著的特异性抑制作用。
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引用次数: 0
NIR-Remote Selectively Triggered Buprenorphine Hydrochloride Release from Microneedle Patches for the Treatment of Neuropathic Pain. 用于治疗神经性疼痛的微针贴片的近红外远程选择性触发盐酸丁丙诺啡释放。
IF 5.4 2区 医学 Q2 MATERIALS SCIENCE, BIOMATERIALS Pub Date : 2024-07-16 DOI: 10.1021/acsbiomaterials.4c00733
Huanzhi Han, Bowen Li, Run Yang, Hao-Lin Guo, Qiuya Li, Hua Wang, Bin Zheng, Yang Bai, Yonghao Yu

Neuropathic pain is a prevalent form of intermittent chronic pain, affecting approximately 7-10% of the global population. However, the current clinical administration methods, such as injection and oral administration, are mostly one-time administration, which cannot achieve accurate control of pain degree and drug dose. Herein, we developed near-infrared (NIR) light-responsive microneedle patches (MNPs) to spatiotemporally control the drug dose released to treat neuropathic pain according to the onset state. The mechanism of action utilizes upconversion nanoparticles to convert NIR light into visible and ultraviolet light. This conversion triggers the rapid rotation of the azobenzene molecular motor in the mesoporous material, enabling the on-demand controlled release of a drug dose. Additionally, MNs are used to overcome the barrier of the stratum corneum in a minimally invasive and painless manner, effectively promoting the transdermal penetration of drug molecules. The effectiveness of these patches has been demonstrated through significant results. Upon exposure to NIR light for five consecutive cycles, with each cycle lasting 30 s, the patches achieved a precise release of 318 μg of medication. In a mouse model, maximum pain relief was observed within 1 h of one cycle of NIR light exposure, with the effects lasting up to 6 h. The same level of precise treatment efficacy was maintained for subsequent pain episodes with similar light exposure. The NIR-controlled drugs precision-released MNPs provide a novel paradigm for the treatment of intermittent neuropathic pain.

神经性疼痛是一种常见的间歇性慢性疼痛,约占全球人口的 7-10%。然而,目前临床上的给药方式,如注射和口服,多为一次性给药,无法实现对疼痛程度和药物剂量的精确控制。在此,我们开发了近红外(NIR)光响应微针贴片(MNPs),可根据发病状态时空控制药物释放剂量,治疗神经病理性疼痛。其作用机制是利用上转换纳米粒子将近红外光转换成可见光和紫外光。这种转换会触发介孔材料中的偶氮苯分子马达快速旋转,从而实现药物剂量的按需控制释放。此外,MNs 还能以微创和无痛的方式克服角质层的屏障,有效促进药物分子的透皮渗透。这些贴片的有效性已通过显著结果得到证明。在连续五个周期(每个周期持续 30 秒)的近红外光照射下,药贴实现了 318 μg 药物的精确释放。在小鼠模型中,一个周期的近红外光照射后 1 小时内就能观察到最大程度的疼痛缓解,效果可持续 6 小时。近红外控制药物精确释放 MNPs 为治疗间歇性神经病理性疼痛提供了一种新的范例。
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引用次数: 0
Conductive/Insulating Bioinks with Multitechnology Compatibility and Adjustable Performance. 具有多种技术兼容性和可调性能的导电/绝缘生物墨水。
IF 5.4 2区 医学 Q2 MATERIALS SCIENCE, BIOMATERIALS Pub Date : 2024-07-16 DOI: 10.1021/acsbiomaterials.4c00631
Xi Yang, Yufan Zhong, Liang Zhang, Yulu Liu, Fengling Zhuo, Jianmin Wang, Linyan Ge, Liuhang Zhang, Xiangyu Zeng, Weiqiang Tan, Guanghui Song, Hua Zhang, Xiaozhi Wang

Conducting/insulating inks have received significant attention for the fabrication of a wide range of additive manufacturing technology. However, current inks often demonstrate poor biocompatibility and face trade-offs between conductivity and mechanical stiffness under physiological conditions. Here, conductive/insulating bioinks based on two-dimensional materials are proposed. The conductive bioink, graphene (GR)-poly(lactic-co-glycolic acid) (PLGA), is prepared by introducing conductive GR into a degradable polymer matrix, PLGA, while the insulating bioink, boron nitride (BN)-PLGA, is synthesized by adding insulating BN. By optimizing the material ratios, this work achieves precise control of the electromechanical properties of the bioinks, thereby enabling the flexible construction of conductive networks according to specific requirements. Furthermore, these bioinks are compatible with a variety of manufacturing technologies such as 3D printing, electrospinning, spin coating, and injection molding, expanding their application range in the biomedical field. Overall, the results suggest that these conducting/insulating bioinks offer improved mechanical, electronic, and biological properties for various emerging biomedical applications.

导电/绝缘墨水在各种增材制造技术的制造过程中受到了极大关注。然而,目前的油墨通常生物相容性较差,在生理条件下面临导电性和机械刚度之间的权衡。在此,我们提出了基于二维材料的导电/绝缘生物墨水。导电生物墨水,即石墨烯(GR)-聚(乳酸-共聚-乙醇酸)(PLGA),是通过将导电石墨烯引入可降解聚合物基质 PLGA 而制备的;而绝缘生物墨水,即氮化硼(BN)-PLGA,则是通过添加绝缘 BN 而合成的。通过优化材料配比,这项工作实现了对生物墨水机电特性的精确控制,从而能够根据特定要求灵活构建导电网络。此外,这些生物墨水还与 3D 打印、电纺丝、旋涂和注塑成型等多种制造技术兼容,从而扩大了它们在生物医学领域的应用范围。总之,研究结果表明,这些导电/绝缘生物墨水具有更好的机械、电子和生物特性,适用于各种新兴的生物医学应用。
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引用次数: 0
Corrosion Resistance of Biodegradable Zinc Surfaces Enhanced by UV-Grafted Polydimethylsiloxane Coating. 紫外线接枝聚二甲基硅氧烷涂层增强可生物降解锌表面的抗腐蚀能力
IF 5.4 2区 医学 Q2 MATERIALS SCIENCE, BIOMATERIALS Pub Date : 2024-07-15 DOI: 10.1021/acsbiomaterials.4c00503
Davide Pupillo, Mark P Bruns, Lucia H Prado, Francesco Di Franco, David Böhringer, Anca Mazare, Wolfgang H Goldmann, Sannakaisa Virtanen, Monica Santamaria, Alexander B Tesler

Improved living conditions have led to an increase in life expectancy worldwide. However, as people age, the risk of vascular disease tends to increase due to the accumulation and buildup of plaque in arteries. Vascular stents are used to keep blood vessels open. Biodegradable stents are designed to provide a temporary support vessel that gradually degrades and is absorbed by the body, leaving behind healed blood vessels. However, biodegradable metals can suffer from reduced mechanical strength and/or inflammatory response, both of which can affect the rate of corrosion. Therefore, it is essential to achieve a controlled and predictable degradation rate. Here, we demonstrate that the corrosion resistance of biodegradable Zn surfaces is improved by electroless deposition of zinc hydroxystannate followed by UV-grafting with silicone oil (PDMS). Potentiodynamic polarization, electrochemical impedance spectroscopy, respiratory kinetic measurements, and long-term immersion in three simulated body fluids were applied. Although zinc hydroxystannate improves the corrosion resistance of Zn to some extent, it introduces a high surface area with hydroxyl units used to UV-graft PDMS molecules. Our results demonstrate that hydrophobic PDMS causes a 3-fold reduction in corrosion of Zn-based materials in biological environments and reduces cytotoxicity through the uncontrolled release of Zn ions.

生活条件的改善使全球人口的预期寿命延长。然而,随着年龄的增长,动脉血管内斑块的积累和堆积会增加罹患血管疾病的风险。血管支架用于保持血管通畅。可生物降解支架的设计目的是提供一个临时的支撑血管,逐渐降解并被人体吸收,留下已愈合的血管。然而,生物可降解金属的机械强度和/或炎症反应可能会降低,两者都会影响腐蚀速度。因此,实现可控、可预测的降解率至关重要。在此,我们证明了通过无电解沉积羟基锡酸锌,然后用硅油(PDMS)进行紫外接枝,可生物降解锌表面的耐腐蚀性能得到了改善。实验采用了电位极化、电化学阻抗光谱、呼吸动力学测量以及在三种模拟体液中长期浸泡等方法。虽然羟基锡酸锌在一定程度上提高了锌的耐腐蚀性,但它与用于紫外接枝 PDMS 分子的羟基单元形成了高表面积。我们的研究结果表明,疏水性 PDMS 可使锌基材料在生物环境中的腐蚀性降低 3 倍,并通过不受控制地释放锌离子降低细胞毒性。
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引用次数: 0
Using Microfluidics to Align Matrix Architecture and Generate Chemokine Gradients Promotes Directional Branching in a Model of Epithelial Morphogenesis. 利用微流体技术调整基质结构并生成趋化因子梯度,促进上皮细胞形态发生模型中的定向分枝。
IF 5.4 2区 医学 Q2 MATERIALS SCIENCE, BIOMATERIALS Pub Date : 2024-07-15 DOI: 10.1021/acsbiomaterials.4c00245
Jessanne Y Lichtenberg, Corinne E Leonard, Hazel R Sterling, Valentina Santos Agreda, Priscilla Y Hwang

The mechanical cue of fiber alignment plays a key role in the development of various tissues in the body. The ability to study the effect of these stimuli in vitro has been limited previously. Here, we present a microfluidic device capable of intrinsically generating aligned fibers using the microchannel geometry. The device also features tunable interstitial fluid flow and the ability to form a morphogen gradient. These aspects allow for the modeling of complex tissues and to differentiate cell response to different stimuli. To demonstrate the abilities of our device, we incorporated luminal epithelial cysts into our device and induced growth factor stimulation. We found the mechanical cue of fiber alignment to play a dominant role in cell elongation and the ability to form protrusions was dependent on cadherin-3. Together, this work serves as a springboard for future potential with these devices to answer questions in developmental biology and complex diseases such as cancers.

纤维排列的机械线索在人体各种组织的发育过程中起着关键作用。此前,体外研究这些刺激作用的能力一直受到限制。在这里,我们展示了一种微流体设备,它能够利用微通道的几何形状内在地生成排列整齐的纤维。该装置还具有可调间隙流体流动和形成形态发生梯度的能力。通过这些方面,可以建立复杂组织的模型,并区分细胞对不同刺激的反应。为了证明我们设备的能力,我们将管腔上皮囊肿纳入设备,并诱导生长因子刺激。我们发现,纤维排列的机械线索在细胞伸长中起着主导作用,而形成突起的能力则取决于粘连蛋白-3。总之,这项工作为未来利用这些装置回答发育生物学和癌症等复杂疾病的问题提供了一个跳板。
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引用次数: 0
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