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Correction to "In Situ Oral Metabolism Analysis of Astringent Compounds in Tea by Paper Spray Mass Spectrometry, Electrospray Mass Spectrometry, Turbidimetry, and Sensory Evaluation". 通过纸喷雾质谱法、电喷雾质谱法、浊度测定法和感官评价对茶叶中的涩味化合物进行原位口服代谢分析》的更正。
IF 5.7 1区 农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2024-07-15 DOI: 10.1021/acs.jafc.4c05707
Qiong Chen, Xin Guan, Zhibin Zhang, Xiaoduo Ma, Tianyang Guo, Huanlu Song
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引用次数: 0
A Novel AHAS-Inhibiting Herbicide Candidate for Controlling Leptochloa chinensis: A Devastating Weedy Grass in Rice Fields. 一种新型 AHAS 抑制性除草剂候选品种,可用于控制 Leptochloa chinensis:稻田中的一种毁灭性杂草
IF 5.7 1区 农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2024-07-15 DOI: 10.1021/acs.jafc.4c03891
Yinglu Chen, Yitao Yan, Jie Chen, Ben Zheng, Youwei Jiang, Zhengzhong Kang, Jun Wu

Given the prevalence of the malignant weed Chinese Sprangletop (Leptochloa chinensis (L.) Nees) in rice fields, the development of novel herbicides against this weed has aroused wide interest. Here, we report a novel diphenyl ether-pyrimidine hybrid, DEP-5, serving as a systematic pre/postemergence herbicide candidate for broad-spectrum weed control in rice fields, specifically for L. chinensis. Notably, DEP-5 exhibits over 80% herbicidal activity against the resistant biotypes even at 37.5 g a.i./ha under greenhouse conditions and has complete control of L. chinensis at 150 g a.i./ha in the rice fields. We uncover that DEP-5 acts as a noncompetitive inhibitor of acetohydroxyacid synthase (AHAS) with an inhibition constant (Ki) of 39.4 μM. We propose that DEP-5 binds to AHAS in two hydrophobic-driven binding modes that differ from commercial AHAS inhibitors. Overall, these findings demonstrate that DEP-5 has great potential to be developed into a herbicide for L. chinensis control and inspire fresh concepts for novel AHAS-inhibiting herbicide design.

鉴于稻田中恶性杂草禾本科杂草(Leptochloa chinensis (L.) Nees)的普遍存在,开发新型除草剂来防除这种杂草引起了广泛的兴趣。在此,我们报告了一种新型二苯醚-嘧啶杂交种 DEP-5,它是一种系统的芽前/芽后除草剂候选品种,可广谱防除稻田杂草,特别是禾本科杂草。值得注意的是,即使在温室条件下使用 37.5 克活性成分/公顷,DEP-5 对抗性生物型仍有 80% 以上的除草活性,而在稻田中使用 150 克活性成分/公顷时,DEP-5 能完全控制禾本科杂草。我们发现 DEP-5 是乙酰羟基酸合成酶(AHAS)的非竞争性抑制剂,其抑制常数(Ki)为 39.4 μM。我们认为 DEP-5 与 AHAS 的结合有两种疏水驱动的结合模式,这两种模式与商用 AHAS 抑制剂不同。总之,这些研究结果表明,DEP-5 有很大的潜力被开发成一种用于防治禾本科植物的除草剂,并为新型 AHAS 抑制除草剂的设计提供了新的思路。
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引用次数: 0
Efficient Secretory Expression and Purification on Three Insoluble Amidohydrolases for Ochratoxin A Hydrolysis by Pichia pastoris. 用毕赤酵母高效表达和纯化三种不溶性酰胺水解酶,以水解赭曲霉毒素 A。
IF 5.7 1区 农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2024-07-14 DOI: 10.1021/acs.jafc.4c03804
Xuanjun Zhang, Xue Ma, Guangqing Dai, Xiaojie Fu, Yu Zhou

As a highly toxic mycotoxin, ochratoxin A (OTA) is widely contaminating agricultural products and has various toxicological effects. Bioenzymes for OTA degradation have shown promising potential for detoxification. Other than the efficient amidohydrolase ADH3 previously, two novel amidohydrolases ADH1 and AMD3 were obtained in this study. During Escherichia coli expression, the expressed protein solubility was very low and will limit future industrial application. Here, high copy number integrations were screened, and the amidohydrolases were efficiently secretory expressed by Pichia pastoris GS115. The protein yields from 1.0 L of fermentation supernatant were 53.5 mg for ADH1, 89.15 mg for ADH3, and 79.5 mg for AMD3. The catalytic efficiency (Kcat/Km) of secretory proteins was 124.95 s-1 mM-1 for ADH3, 123.21 s-1 mM-1 for ADH1, and 371.99 s-1 mM-1 for AMD3. In comparison to E. coli expression, the active protein yields substantially increased 15.78-51.53 times. Meanwhile, two novel amidohydrolases (ADH1 and AMD3) showed much higher activity than ADH3 that produced by secretory expression.

作为一种剧毒霉菌毒素,赭曲霉毒素 A(OTA)广泛污染农产品,并具有多种毒理效应。降解赭曲霉毒素 A 的生物酶在解毒方面显示出巨大的潜力。除了之前发现的高效酰胺水解酶 ADH3 外,本研究还发现了两种新型酰胺水解酶 ADH1 和 AMD3。在大肠杆菌表达过程中,所表达的蛋白质溶解度非常低,这将限制未来的工业应用。本研究筛选了高拷贝数整合物,并用 Pichia pastoris GS115 高效分泌表达了酰胺水解酶。1.0 升发酵上清液的蛋白质产量分别为:ADH1 53.5 毫克、ADH3 89.15 毫克和 AMD3 79.5 毫克。分泌蛋白的催化效率(Kcat/Km)分别为:ADH3 124.95 s-1 mM-1、ADH1 123.21 s-1 mM-1、AMD3 371.99 s-1 mM-1。与大肠杆菌表达相比,活性蛋白产量大幅提高了 15.78-51.53 倍。同时,两种新型酰胺水解酶(ADH1 和 AMD3)的活性远高于分泌型表达的 ADH3。
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引用次数: 0
Genome-Wide Association Study Reveals the Genetic Basis of Crude Fiber Components in Brassica napus L. Shoots at Stem Elongation Stage 全基因组关联研究揭示甘蓝型油菜茎伸长期嫩芽粗纤维成分的遗传基础
IF 6.1 1区 农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2024-07-13 DOI: 10.1021/acs.jafc.4c03032
Rui Shi, Yu Cao, Tinghai Yang, Yaping Wang, Yanan Xiang, Feng Chen, Wei Zhang, Xiaoying Zhou, Chengming Sun, Sanxiong Fu, Maolong Hu, Jiefu Zhang, Xiaodong Wang
Brassica napus is currently the principal field crop for producing materials for primary, secondary and tertiary industries. B. napus shoots at stem elongation stage are rich in anthocyanins, vitamin C and mineral elements such as selenium, calcium and zinc, and represent a new type of green vegetable. However, the high crude fiber (CF) content of B. napus shoots affects their taste, and few studies have focused on the quality traits of these vegetables. In this study, we investigated five traits related to the CF components, including neutral detergent fiber (NDF), acid detergent fiber (ADF), acid detergent lignin (ADL), hemicellulose (Hem) and cellulose (Cel), of B. napus shoots. Whole-genome resequencing at a depth of ∼20× was utilized to genotype an association panel of 202 diverse accessions, which resulted in the identification of 6,093,649 single nucleotide polymorphisms (SNPs) and 996,252 indels, respectively. A genome-wide association study (GWAS) was performed for the five CF-related traits based on the phenotypic data observed in four environments. A total of 1,285 significant SNPs were detected at the threshold of -log10 (p) = 5.16, and 97 significant association regions were obtained. In addition, seven candidate genes located on chromosomes A2 (one gene), A8 (three genes), A9 (two genes) and C9 (one gene) related to CF traits were identified, and ten lines containing low CF contents were selected as excellent germplasm resources for breeding. Our results contributed new insights into the genetic basis of CF traits and suggested germplasm resources for the quality improvement of B. napus shoots.
油菜是目前生产第一、第二和第三产业材料的主要大田作物。处于茎伸长阶段的油菜嫩芽富含花青素、维生素 C 和硒、钙、锌等矿物质元素,是一种新型绿色蔬菜。然而,油菜芽的粗纤维(CF)含量较高,影响了其口感,很少有研究关注这类蔬菜的品质性状。在这项研究中,我们调查了五种与油菜芽的粗纤维成分有关的性状,包括中性洗涤纤维(NDF)、酸性洗涤纤维(ADF)、酸性洗涤木质素(ADL)、半纤维素(Hem)和纤维素(Cel)。利用深度为 20× 的全基因组重测序技术对 202 个不同品种进行了基因分型,分别鉴定出 6,093,649 个单核苷酸多态性(SNPs)和 996,252 个嵌合体。根据在四种环境中观察到的表型数据,对五种 CF 相关性状进行了全基因组关联研究(GWAS)。在-log10 (p) = 5.16的阈值下,共检测到1,285个显著的SNPs,并获得了97个显著的关联区域。此外,还发现了位于染色体 A2(1 个基因)、A8(3 个基因)、A9(2 个基因)和 C9(1 个基因)上与 CF 性状相关的 7 个候选基因,并筛选出 10 个 CF 含量低的品系作为优良种质资源用于育种。我们的研究结果有助于深入了解CF性状的遗传基础,并为提高油菜芽的品质提供了种质资源。
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引用次数: 0
Exploring the Health Benefits of Boletus aereus Polysaccharides: Extraction, Structural Characterization, and Antiproliferative Properties against Non-Hodgkin's Lymphomas (NHLs). 探索牛肝菌多糖对健康的益处:牛肝菌多糖的提取、结构特征和对非霍奇金淋巴瘤(NHLs)的抗增殖特性。
IF 5.7 1区 农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2024-07-12 DOI: 10.1021/acs.jafc.4c03945
Haidi Wang, Linyu Yang, Qiang Qiu, Qianru Rao, Li Liu, Yuchen Cui, Liang Zhang, Yucheng Ma, Xi Jin, Dongxue Yang, Shiqian Qi, Kunjie Wang, Yong Li, Xufeng Zhang, Min Zhao

Boletus aereus Fr. ex Bull. stands out as a delectable edible mushroom with high nutritional and medicinal values, featuring polysaccharides as its primary nutrient composition. In our continuous exploration of its beneficial substances, a novel polysaccharide (BAPN-1) with a molecular weight of 2279 kDa was prepared. It was identified as a glucan with a backbone composed of the residues →4)-α-Glcp-(1→ and →4,6)-α-Glcp-(1→ connected in a proportion of 5:1 and a β-Glcp-(1→ side residue attached at C6 of the →4,6)-α-Glcp-(1→ residue. Biologically, BAPN-1 exhibited broad-spectrum antiproliferative activities against various NHL cells, including HuT-78, OCI-LY1, OCI-LY18, Jurkat, RL, and Karpas-299, with IC50 values of 0.73, 1.21, 3.18, 1.52, 3.34, and 4.25 mg/mL, respectively. Additionally, BAPN-1 significantly induced cell cycle arrest in the G2/M phase and caused apoptosis of NHL cells. Mechanistically, bulk RNA sequencing and Western blot analysis revealed that BAPN-1 could upregulate cyclin B1 and enhance cleaved caspase-9 expression through the inhibition of FGFR3 and RAF-MEK-ERK signaling pathways. This work supports the improved utilization of B. aereus in high-value health products.

牛肝菌(Boletus aereus Fr. ex Bull.在不断探索其有益物质的过程中,我们制备出了一种分子量为 2279 kDa 的新型多糖(BAPN-1)。经鉴定,BAPN-1 是一种葡聚糖,其骨架由 →4)-α-Glcp-(1→和 →4,6)-α-Glcp-(1→以 5:1 的比例连接而成,在 →4,6)-α-Glcp-(1→残基的 C6 处连接有一个 β-Glcp-(1→ 侧残基。生物学上,BAPN-1 对各种 NHL 细胞(包括 HuT-78、OCI-LY1、OCI-LY18、Jurkat、RL 和 Karpas-299)具有广谱抗增殖活性,IC50 值分别为 0.73、1.21、3.18、1.52、3.34 和 4.25 mg/mL。此外,BAPN-1 还能显著诱导细胞周期停滞在 G2/M 期,并导致 NHL 细胞凋亡。从机理上讲,大量 RNA 测序和 Western 印迹分析表明,BAPN-1 可通过抑制 FGFR3 和 RAF-MEK-ERK 信号通路,上调细胞周期蛋白 B1 并增强裂解的 Caspase-9 表达。这项研究成果有助于提高 B. aereus 在高价值保健产品中的利用率。
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引用次数: 0
Characterization of Eurotium cristatum Fermented Thinned Young Apple and Mechanisms Underlying Its Alleviating Impacts on Experimental Colitis. 冠突散囊菌发酵变薄嫩苹果的特性及其缓解实验性结肠炎的机制
IF 5.7 1区 农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2024-07-12 DOI: 10.1021/acs.jafc.4c02005
Wei Song, Lanqi Zhou, Tianqi Liu, Guoze Wang, Jiayao Lv, Shiyi Zhang, Xiaoshuang Dai, Meng Wang, Lin Shi

A hundred million tons of young apples are thinned and discarded in the orchard per year, aiming to increase the yield and quality of apples. We fermented thinned young apples using a potential probiotic fungus, Eurotium cristatum, which notably disrupted the microstructure of raw samples, as characterized by the scanning electron microscope. Fermentation substantially altered the metabolite profiles of samples, which are predicted to alleviate colitis via regulating inflammatory response and response to lipopolysaccharide by using network pharmacology analysis. In vivo, oral gavage of water extracts of E. cristatum fermented young apples (E.YAP) effectively alleviated DSS-induced colitis, restored the histopathology damage, reduced the levels of inflammatory cytokines, and promoted colonic expressions of tight junction proteins. Moreover, E.YAP ameliorated gut dysbacteriosis by increasing abundances of Lactobacillus,Blautia, Muribaculaceae, and Prevotellaceae_UCG-001 while inhibiting Turicibacter, Alistipes, and Desulfovibrio. Importantly, E.YAP increased colonic bile acids, such as CA, TCA, DCA, TUDCA, and LCA, thereby alleviating colitis via PXR/NF-κB signaling. Furthermore, a synbiotic combination with Limosilactobacillus reuteri WX-94, a probiotic strain isolated from feces of healthy individuals with anti-inflammatory properties, augmented anticolitis capacities of E.YAP. Our findings demonstrate that E.YAP could be a novel, potent, food-based anti-inflammatory prebiotic for relieving inflammatory injuries.

为了提高苹果的产量和质量,每年有一亿吨幼苹果被疏果后丢弃在果园里。我们使用一种潜在的益生菌--Eurotium cristatum--对疏果后的幼苹果进行发酵,通过扫描电子显微镜观察,这种益生菌明显破坏了原始样品的微观结构。发酵大大改变了样品的代谢物谱,通过网络药理学分析,这些代谢物可通过调节炎症反应和对脂多糖的反应来缓解结肠炎。在体内,口服E. cristatum发酵幼苹果水提取物(E.YAP)可有效缓解DSS诱导的结肠炎,恢复组织病理学损伤,降低炎性细胞因子水平,促进结肠紧密连接蛋白的表达。此外,E.YAP 还能提高乳酸杆菌、布劳氏菌、穆里巴库科菌和前螺旋体_UCG-001 的丰度,同时抑制图里茨菌、阿利斯蒂普斯菌和脱硫弧菌,从而改善肠道菌群失调。重要的是,E.YAP 增加了结肠胆汁酸,如 CA、TCA、DCA、TUDCA 和 LCA,从而通过 PXR/NF-κB 信号传导缓解了结肠炎。此外,与从健康人粪便中分离出的具有抗炎特性的益生菌株--Limosilactobacillus reuteri WX-94--的合成益生菌组合增强了 E.YAP 的抗结肠炎能力。我们的研究结果表明,E.YAP 可作为一种新型、强效、基于食物的抗炎益生元,用于缓解炎症损伤。
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引用次数: 0
Bta-miR-200a Regulates Milk Fat Biosynthesis by Targeting IRS2 to Inhibit the PI3K/Akt Signal Pathway in Bovine Mammary Epithelial Cells. Bta-miR-200a 通过靶向 IRS2 抑制牛乳腺上皮细胞中的 PI3K/Akt 信号通路来调节乳脂的合成。
IF 5.7 1区 农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2024-07-12 DOI: 10.1021/acs.jafc.4c02508
Jianbing Tan, Benshun Yang, Liang Qiu, Ruiying He, Zhangqing Wu, Miaomiao Ye, Linsen Zan, Wucai Yang

Milk fat synthesis has garnered significant attention due to its influence on the quality of milk. Recently, an increasing amount of proofs have elucidated that microRNAs (miRNAs) are important post-transcriptional factor involved in regulating gene expression and play a significant role in milk fat synthesis. MiR-200a was differentially expressed in the mammary gland tissue of dairy cows during different lactation periods, which indicated that miR-200a was a candidate miRNA involved in regulating milk fat synthesis. In our research, we investigated the potential function of miR-200a in regulating milk fat biosynthesis in bovine mammary epithelial cells (BMECs). We discovered that miR-200a inhibited cellular triacylglycerol (TAG) synthesis and suppressed lipid droplet formation; at the same time, miR-200a overexpression suppressed the mRNA and protein expression of milk fat metabolism-related genes, such as fatty acid synthase (FASN), peroxisome proliferator-activated receptor gamma (PPARγ), sterol regulatory element-binding protein 1 (SREBP1), CCAAT enhancer binding protein alpha (CEBPα), etc. However, knocking down miR-200a displayed the opposite results. We uncovered that insulin receptor substrate 2 (IRS2) was a candidate target gene of miR-200a through the bioinformatics online program TargetScan. Subsequently, it was confirmed that miR-200a directly targeted the 3'-untranslated region (3'-UTR) of IRS2 via real-time fluorescence quantitative PCR (RT-qPCR), western blot analysis, and dual-luciferase reporter gene assay. Additionally, IRS2 knockdown in BMECs has similar effects to miR-200a overexpression. Our research set up the mechanism by which miR-200a interacted with IRS2 and discovered that miR-200a targeted IRS2 and modulated the activity of the PI3K/Akt signaling pathway, thereby taking part in regulating milk fat synthesis in BMECs. Our research results provided valuable information on the molecular mechanisms for enhancing milk quality from the view of miRNA-mRNA regulatory networks.

乳脂合成因其对牛奶质量的影响而备受关注。最近,越来越多的证据表明,微RNA(miRNA)是参与调控基因表达的重要转录后因子,在乳脂合成中发挥着重要作用。MiR-200a在不同泌乳期奶牛乳腺组织中的表达存在差异,这表明miR-200a是参与调控乳脂合成的候选miRNA。我们研究了 miR-200a 在牛乳腺上皮细胞(BMECs)中调控乳脂生物合成的潜在功能。我们发现,miR-200a能抑制细胞三酰甘油(TAG)的合成,抑制脂滴的形成;同时,miR-200a的过表达能抑制乳脂代谢相关基因,如脂肪酸合成酶(FASN)、过氧化物酶体增殖激活受体γ(PPARγ)、甾醇调节元件结合蛋白1(SREBP1)、CCAAT增强子结合蛋白α(CEBPα)等的mRNA和蛋白表达。然而,敲除 miR-200a 则显示出相反的结果。我们通过生物信息学在线程序 TargetScan 发现,胰岛素受体底物 2(IRS2)是 miR-200a 的候选靶基因。随后,通过实时荧光定量 PCR(RT-qPCR)、Western 印迹分析和双荧光素酶报告基因检测,证实 miR-200a 直接靶向 IRS2 的 3'- 非翻译区(3'-UTR)。此外,在 BMECs 中敲除 IRS2 与过表达 miR-200a 有相似的效果。我们的研究建立了miR-200a与IRS2相互作用的机制,发现miR-200a以IRS2为靶点,调节PI3K/Akt信号通路的活性,从而参与调控BMECs的乳脂合成。我们的研究成果从miRNA-mRNA调控网络的角度为提高牛奶质量的分子机制提供了有价值的信息。
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引用次数: 0
Regulatory Peptide Encoded by the Primary Transcript of miR396a Influences Gene Expression and Root Development in Solanum lycopersicum. miR396a 主转录本编码的调控肽影响茄果类植物的基因表达和根系发育
IF 5.7 1区 农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2024-07-12 DOI: 10.1021/acs.jafc.4c03588
Zhengjie Wang, Ruili Lv, Chenglin Su, Yan Li, Sizhe Fang, Ruirui Yang, Jiaxuan Zhu, Ruiming Wang, Jun Meng, Yushi Luan

MicroRNAs (miRNAs) are the processing products of primary miRNAs (pri-miRNAs) that regulate the expression of target genes. Recent studies have demonstrated that some pri-miRNAs can encode small peptides (miPEPs) that perform significant biological functions. The function of miPEPs in tomatoes, an important model horticultural crop, remains to be investigated. Here, we characterized the primary sequence of tomato miR396a using 5' RACE and confirmed the presence of miPEP396a in tomato by verifying the translational activity of the start codon. It primarily resides in the nucleus to exert its function and additionally regulates the expression of pri-miR396a, miR396a, and its target genes. Transcriptomic and metabolomic analyses showed that in vitro synthesis of miPEP396a significantly increased the expression of genes related to phenylpropanoid biosynthesis and hormones in tomato. Meanwhile, our in vitro application of miPEP396a in tomato significantly inhibited the elongation of tomato primary roots. In conclusion, our results indicate that miPEP396a regulates root growth in tomato by specifically promoting miR396a expression, provide insight into the function of miPEPs in tomato and potential applications.

微小RNA(miRNA)是初级miRNA(pri-miRNA)的加工产物,可调节靶基因的表达。最近的研究表明,一些 pri-miRNAs 可以编码小肽(miPEPs),从而发挥重要的生物学功能。miPEPs在番茄这种重要的示范园艺作物中的功能仍有待研究。在此,我们利用 5' RACE 鉴定了番茄 miR396a 的主序列,并通过验证起始密码子的翻译活性证实了 miPEP396a 在番茄中的存在。它主要驻留在细胞核中发挥功能,此外还调控 pri-miR396a、miR396a 及其靶基因的表达。转录组和代谢组分析表明,体外合成 miPEP396a 能显著提高番茄中与苯丙类生物合成和激素相关基因的表达。同时,在番茄体外应用 miPEP396a 能明显抑制番茄主根的伸长。总之,我们的研究结果表明,miPEP396a通过特异性地促进miR396a的表达来调控番茄根的生长,为了解miPEPs在番茄中的功能和潜在应用提供了启示。
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引用次数: 0
Cadmium Maximum Levels and Residue Situation in the German Wheat and Rye Harvest from 1975 to 2021. 1975 年至 2021 年德国小麦和黑麦收成中的镉最高含量和残留情况。
IF 5.7 1区 农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2024-07-12 DOI: 10.1021/acs.jafc.4c03560
Alexandra Hüsken, Lidia Arent, Regina Lohmayer

For a better understanding of cadmium (Cd) accumulation over long time periods in cereals, Cd levels of the German wheat and rye harvest from 1975 to 2021 were analyzed. Overall, wheat had higher grain Cd concentrations than rye. Comparing mean values from different time periods showed that Cd levels in winter rye have stabilized, while Cd concentrations in winter wheat have decreased. Furthermore, Cd concentrations in almost all samples were below the newly introduced European Commission limits specifying the maximum permissible contaminant levels in foodstuffs (Cd in grains: rye 50 μg/kg FW; wheat 100 μg/kg FW). However, it is important to note that Cd is still ubiquitous in the German wheat and rye harvest. Although there has been a significant reduction in emissions and imissions for around 30 years, the extraordinarily long biological half-life and carcinogenicity of Cd still make it a relevant substance to food safety and human health.

为了更好地了解镉(Cd)在谷物中长期积累的情况,我们分析了 1975 年至 2021 年德国小麦和黑麦收获时的镉含量。总体而言,小麦的谷物镉浓度高于黑麦。比较不同时期的平均值发现,冬黑麦的镉含量趋于稳定,而冬小麦的镉含量则有所下降。此外,几乎所有样本中的镉浓度都低于欧盟委员会新出台的规定食品中最大允许污染物含量的限值(谷物中的镉:黑麦 50 μg/kg FW;小麦 100 μg/kg FW)。不过,值得注意的是,在德国收获的小麦和黑麦中,镉仍然无处不在。尽管 30 年来,镉的排放量和隐含量已大幅减少,但由于镉的生物半衰期极长且具有致癌性,因此它仍然是一种与食品安全和人类健康息息相关的物质。
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引用次数: 0
Polyunsaturated Fatty Acids in Quinoa Induce Ferroptosis of Colon Cancer by Suppressing Stemness. 藜麦中的多不饱和脂肪酸通过抑制干性诱导结肠癌的铁变态反应
IF 5.7 1区 农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2024-07-11 DOI: 10.1021/acs.jafc.4c00118
Songtao Li, Man Ding, Mangmang Feng, Xiaxia Fan, Zhuoyu Li

Polyunsaturated fatty acids (PUFAs) are essential nutrients for the human body, playing crucial roles in reducing blood lipids, anti-inflammatory responses, and anticancer effect. Quinoa is a nutritionally sound food source, rich in PUFAs. This study investigates the role of quinoa polyunsaturated fatty acids (QPAs) on quelling drug resistance in colorectal cancer. The results reveal that QPA downregulates the expression of drug-resistant proteins P-gp, MRP1, and BCRP, thereby enhancing the sensitivity of colorectal cancer drug-resistant cells to the chemotherapy drug. QPA also inhibits the stemness of drug-resistant colorectal cancer cells by reducing the expression of the stemness marker CD44. Consequently, it suppresses the downstream protein SLC7A11 and leads to ferroptosis. Additionally, QPA makes the expression of ferritin lower and increases the concentration of free iron ions within cells, leading to ferroptosis. Overall, QPA has the dual-function reversing drug resistance in colorectal cancer by simultaneously inhibiting stemness and inducing ferroptosis. This study provides a new option for chemotherapy sensitizers and establishes a theoretical foundation for the development and utilization of quinoa.

多不饱和脂肪酸(PUFA)是人体必需的营养素,在降低血脂、抗炎和抗癌方面发挥着重要作用。藜麦是一种营养丰富的食物来源,富含 PUFAs。本研究探讨了藜麦多不饱和脂肪酸(QPA)对抑制结直肠癌耐药性的作用。结果发现,QPA能下调耐药蛋白P-gp、MRP1和BCRP的表达,从而提高结直肠癌耐药细胞对化疗药物的敏感性。QPA 还能通过减少干性标志物 CD44 的表达来抑制耐药结直肠癌细胞的干性。因此,它抑制了下游蛋白 SLC7A11 并导致铁变态反应。此外,QPA 还能降低铁蛋白的表达,增加细胞内游离铁离子的浓度,从而导致铁突变。总之,QPA具有双重功能,可同时抑制干性和诱导铁变态,从而逆转结直肠癌的耐药性。这项研究为化疗增敏剂提供了一种新的选择,并为开发和利用藜麦奠定了理论基础。
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引用次数: 0
期刊
Journal of Agricultural and Food Chemistry
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