首页 > 最新文献

Lab on a Chip最新文献

英文 中文
Outstanding Reviewers for Lab on a Chip in 2023. 2023 年芯片实验室杰出评审员。
IF 6.1 2区 工程技术 Q1 BIOCHEMICAL RESEARCH METHODS Pub Date : 2024-07-15 DOI: 10.1039/d4lc90056a

We would like to take this opportunity to thank all of Lab on a Chip's reviewers for helping to preserve quality and integrity in the chemical science literature. We would also like to highlight our Outstanding Reviewers for Lab on a Chip in 2023.

我们想借此机会感谢 Lab on a Chip 的所有审稿人,感谢他们帮助维护化学科学文献的质量和完整性。此外,我们还想重点介绍一下 2023 年芯片实验室的杰出审稿人。
{"title":"Outstanding Reviewers for <i>Lab on a Chip</i> in 2023.","authors":"","doi":"10.1039/d4lc90056a","DOIUrl":"https://doi.org/10.1039/d4lc90056a","url":null,"abstract":"<p><p>We would like to take this opportunity to thank all of <i>Lab on a Chip</i>'s reviewers for helping to preserve quality and integrity in the chemical science literature. We would also like to highlight our Outstanding Reviewers for <i>Lab on a Chip</i> in 2023.</p>","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141615306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Electric field temporal interference stimulation of neurons in vitro. 体外神经元的电场时间干扰刺激。
IF 6.1 2区 工程技术 Q1 BIOCHEMICAL RESEARCH METHODS Pub Date : 2024-07-12 DOI: 10.1039/d4lc00224e
Annika Ahtiainen, Lilly Leydolph, Jarno M A Tanskanen, Alexander Hunold, Jens Haueisen, Jari A K Hyttinen

Electrical stimulation (ES) techniques, such as deep brain and transcranial electrical stimulation, have shown promise in alleviating the symptoms of depression and other neurological disorders in vivo. A new noninvasive ES method called temporal interference stimulation (TIS), possesses great potential as it can be used to steer the stimulation and possibly selectively modulate different brain regions. To study TIS in a controlled environment, we successfully established an in vitro 'TIS on a chip' setup using rat cortical neurons on microelectrode arrays (MEAs) in combination with a current stimulator. We validated the developed TIS system and demonstrated the spatial steerability of the stimulation by direct electric field measurements in the chip setup. We stimulated cultures of rat cortical neurons at 28 days in vitro (DIV) by two-channel stimulation delivering 1) TIS at 653 Hz and 643 Hz, resulting in a 10 Hz frequency envelope, 2) low-frequency stimulation (LFS) at 10 Hz and 3) high-frequency stimulation (HFS) at 653 Hz. Unstimulated cultures were used as control/sham. We observed the differences in the electric field strengths during TIS, HFS, and LFS. Moreover, HFS and LFS had the smallest effects on neuronal activity. Instead, TIS elicited neuronal electrophysiological responses, especially 24 hours after stimulation. Our 'TIS on a chip' approach eludicates the applicability of TIS as a method to modulate neuronal electrophysiological activity. The TIS on a chip approach provides spatially steerable stimuli while mitigating the effects of high stimulus fields near the stimulation electrodes. Thus, the approach opens new avenues for stimulation on a chip applications, allowing the study of neuronal responses to gain insights into the potential clinical applications of TIS in treating various brain disorders.

脑深部电刺激和经颅电刺激等电刺激(ES)技术在减轻抑郁症和其他神经系统疾病的症状方面已显示出良好的前景。一种名为颞叶干扰刺激(TIS)的新型无创脑电刺激方法具有巨大的潜力,因为它可用于引导刺激,并可能选择性地调节不同的脑区。为了在受控环境中研究 TIS,我们利用微电极阵列(MEA)上的大鼠皮质神经元结合电流刺激器,成功建立了体外 "芯片上的 TIS "装置。我们验证了所开发的 TIS 系统,并通过芯片装置中的直接电场测量证明了刺激的空间可转向性。我们对体外培养 28 天(DIV)的大鼠大脑皮层神经元进行了双通道刺激:1)653 Hz 和 643 Hz 的 TIS,产生 10 Hz 频率包络;2)10 Hz 的低频刺激(LFS);3)653 Hz 的高频刺激(HFS)。未受刺激的培养物被用作对照组。我们观察到 TIS、HFS 和 LFS 期间电场强度的差异。此外,HFS 和 LFS 对神经元活动的影响最小。相反,TIS 会引起神经元电生理反应,尤其是在刺激 24 小时后。我们的 "芯片上的 TIS "方法阐明了 TIS 作为神经元电生理活动调节方法的适用性。芯片上的 TIS 方法提供了空间可调的刺激,同时减轻了刺激电极附近高刺激场的影响。因此,这种方法为芯片上的刺激应用开辟了新途径,使神经元反应研究能够深入了解 TIS 在治疗各种脑部疾病方面的潜在临床应用。
{"title":"Electric field temporal interference stimulation of neurons <i>in vitro</i>.","authors":"Annika Ahtiainen, Lilly Leydolph, Jarno M A Tanskanen, Alexander Hunold, Jens Haueisen, Jari A K Hyttinen","doi":"10.1039/d4lc00224e","DOIUrl":"https://doi.org/10.1039/d4lc00224e","url":null,"abstract":"<p><p>Electrical stimulation (ES) techniques, such as deep brain and transcranial electrical stimulation, have shown promise in alleviating the symptoms of depression and other neurological disorders <i>in vivo</i>. A new noninvasive ES method called temporal interference stimulation (TIS), possesses great potential as it can be used to steer the stimulation and possibly selectively modulate different brain regions. To study TIS in a controlled environment, we successfully established an <i>in vitro</i> 'TIS on a chip' setup using rat cortical neurons on microelectrode arrays (MEAs) in combination with a current stimulator. We validated the developed TIS system and demonstrated the spatial steerability of the stimulation by direct electric field measurements in the chip setup. We stimulated cultures of rat cortical neurons at 28 days <i>in vitro</i> (DIV) by two-channel stimulation delivering 1) TIS at 653 Hz and 643 Hz, resulting in a 10 Hz frequency envelope, 2) low-frequency stimulation (LFS) at 10 Hz and 3) high-frequency stimulation (HFS) at 653 Hz. Unstimulated cultures were used as control/sham. We observed the differences in the electric field strengths during TIS, HFS, and LFS. Moreover, HFS and LFS had the smallest effects on neuronal activity. Instead, TIS elicited neuronal electrophysiological responses, especially 24 hours after stimulation. Our 'TIS on a chip' approach eludicates the applicability of TIS as a method to modulate neuronal electrophysiological activity. The TIS on a chip approach provides spatially steerable stimuli while mitigating the effects of high stimulus fields near the stimulation electrodes. Thus, the approach opens new avenues for stimulation on a chip applications, allowing the study of neuronal responses to gain insights into the potential clinical applications of TIS in treating various brain disorders.</p>","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141588954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Early detection of hypo/hyperglycemia using microneedles electrode array-based biosensor for glucose ultrasensitive monitoring in interstitial fluid 使用基于微针电极阵列的生物传感器及早检测低血糖/高血糖,对组织间液中的葡萄糖进行超灵敏监测
IF 6.1 2区 工程技术 Q1 BIOCHEMICAL RESEARCH METHODS Pub Date : 2024-07-10 DOI: 10.1039/d4lc00365a
Samar Elbahy, Mohamed Mansour, Ahmed Soltan, Alyaa Ibrahem Salim
Diabetes is a common chronic metabolic disease with a wide range of clinical symptoms and consequences and one of the main causes of death. For the management of diabetes, painless and continuous interstitial fluid (ISF) glucose monitoring is ideal. Here, we demonstrate the continuous diabetes monitoring using an integrated microneedles (MNs) biosensor with emergency alert system. MN is a novel technique in the field of biomedical engineering because of its ability to analyze bioinformation with minimal invasion. In this work we developed a poly(methyl methacrylate) (PMMA) based MNs glucose sensor. The device was produced by 3D printing technique, microfabrication, electrodeposition, and enzyme immobilization step. color{blue} The in vitro test for the glucose MN sensor showed a linear range from 1.5 to 14 mM with a sensitivity of 1.51 $mu$A/mM, limit of detection (LOD) of 0.35 mM and a good selectivity color{black}. Highly repeatable sensing is observed with good reproducibility. The interference-free detection of glucose in the presence of physiologically relevant concentrations of ascorbic acid, uric acid, and mannose is demonstrated, along with the operational stability of the array. After resolving the biofouling consequences linked to on-body sensing, this MN platform would be appealing for color{red} minimally invasive color{black} electrochemical glucose monitoring. An alert is sent to confidants via email or SMS when these values are abnormal. The application is also able to display the recorded values in the form of a graph to help determine the state of health of the user over a period of time. It can be ended up that continuous monitoring and an emergency alert system is important for keeping an eye on diabetic patients and can alert in case of an abnormal situation of the patient.
糖尿病是一种常见的慢性代谢性疾病,具有多种临床症状和后果,也是导致死亡的主要原因之一。要治疗糖尿病,理想的方法是进行无痛、连续的组织间液(ISF)葡萄糖监测。在这里,我们展示了利用集成微针(MNs)生物传感器和紧急报警系统对糖尿病进行连续监测的方法。微针是生物医学工程领域的一项新技术,因为它能以最小的侵入量分析生物信息。在这项工作中,我们开发了一种基于聚甲基丙烯酸甲酯(PMMA)的 MNs 葡萄糖传感器。该装置是通过三维打印技术、微细加工、电沉积和酶固定步骤制作而成的。葡萄糖 MN 传感器的体外测试表明,其线性范围为 1.5 至 14 mM,灵敏度为 1.51 美元/mu$A/mM,检测限(LOD)为 0.35 mM,并具有良好的选择性。检测结果具有很高的重复性和再现性。在存在生理相关浓度的抗坏血酸、尿酸和甘露糖的情况下,葡萄糖的无干扰检测以及阵列的运行稳定性都得到了证实。在解决了与体外传感相关的生物污染问题后,这种 MN 平台将成为微创电化学葡萄糖监测的理想选择。当这些值出现异常时,会通过电子邮件或短信向知情人发出警报。应用程序还能以图表的形式显示记录的数值,以帮助确定用户在一段时间内的健康状况。由此可见,持续监测和紧急报警系统对于密切关注糖尿病患者非常重要,可以在患者出现异常情况时发出警报。
{"title":"Early detection of hypo/hyperglycemia using microneedles electrode array-based biosensor for glucose ultrasensitive monitoring in interstitial fluid","authors":"Samar Elbahy, Mohamed Mansour, Ahmed Soltan, Alyaa Ibrahem Salim","doi":"10.1039/d4lc00365a","DOIUrl":"https://doi.org/10.1039/d4lc00365a","url":null,"abstract":"Diabetes is a common chronic metabolic disease with a wide range of clinical symptoms and consequences and one of the main causes of death. For the management of diabetes, painless and continuous interstitial fluid (ISF) glucose monitoring is ideal. Here, we demonstrate the continuous diabetes monitoring using an integrated microneedles (MNs) biosensor with emergency alert system. MN is a novel technique in the field of biomedical engineering because of its ability to analyze bioinformation with minimal invasion. In this work we developed a poly(methyl methacrylate) (PMMA) based MNs glucose sensor. The device was produced by 3D printing technique, microfabrication, electrodeposition, and enzyme immobilization step. color{blue} The in vitro test for the glucose MN sensor showed a linear range from 1.5 to 14 mM with a sensitivity of 1.51 $mu$A/mM, limit of detection (LOD) of 0.35 mM and a good selectivity color{black}. Highly repeatable sensing is observed with good reproducibility. The interference-free detection of glucose in the presence of physiologically relevant concentrations of ascorbic acid, uric acid, and mannose is demonstrated, along with the operational stability of the array. After resolving the biofouling consequences linked to on-body sensing, this MN platform would be appealing for color{red} minimally invasive color{black} electrochemical glucose monitoring. An alert is sent to confidants via email or SMS when these values are abnormal. The application is also able to display the recorded values in the form of a graph to help determine the state of health of the user over a period of time. It can be ended up that continuous monitoring and an emergency alert system is important for keeping an eye on diabetic patients and can alert in case of an abnormal situation of the patient.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141566048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Multioxide combinatorial libraries: fusing synthetic approaches and additive technologies for highly orthogonal electronic noses 多氧化物组合文库:融合合成方法和添加技术,打造高度正交的电子鼻
IF 6.1 2区 工程技术 Q1 BIOCHEMICAL RESEARCH METHODS Pub Date : 2024-07-08 DOI: 10.1039/d4lc00252k
Vishalkumar Rajeshbhai Gohel, Margarita Chetyrkina, Andrey Gaev, Nikolay P. Simonenko, Tatiana L Simonenko, Philipp Yu Gorobtsov, Nikita Fisenko, Darya A. Dudorova, Valeriy Zaytsev, Anna Lantsberg, Elizaveta P. Simonenko, Albert G. Nasibulin, Fedor Fedorov
This study evaluates the performance advancement of electronic noses, on-chip engineered multisensor systems, exploiting a combinatorial approach. We analyze a spectrum of metal oxide semiconductor materials produced by individual methods of liquid-phase synthesis and a combination of chemical deposition, and sol-gel method, with hydrothermal treatment. These methodologies are demonstrated to enable obtaining a fairly wide range of nanomaterials that differ significantly in chemical composition, crystal structure, and morphological features. While synthesis routes foster diversity in materials properties, microplotter printing ensures targeted precision in making on-chip arrays for evaluation of combinatorial selectivity concept in the task of organic vapors, like alcohol homologs, acetone, and benzene, classification. The synthesized nanomaterials demonstrate a high chemiresistive response, with limit of detection beyond a ppm level. A specific combination of materials is demonstrated to be relevant when the number of sensors is low, however, such importance diminishes with an increase in the sensor number. We show that on-chip material combinations could favor selectivity to a specific analyte, disregarding the others. Hence, modern synthesis methods, and printing protocols supported by combinatorial analysis might pave the way for fabricating on-the-chip orthogonal multisensor systems.
本研究利用组合方法评估了电子鼻、片上工程多传感器系统的性能进步。我们分析了通过液相合成法、化学沉积法和溶胶-凝胶法结合水热处理法生产的金属氧化物半导体材料。结果表明,这些方法可以获得相当广泛的纳米材料,这些材料在化学成分、晶体结构和形态特征方面都有显著差异。合成路线促进了材料特性的多样性,而微绘图仪打印技术则确保了制作片上阵列的目标精确性,以便在有机蒸汽(如酒精同系物、丙酮和苯)分类任务中评估组合选择性概念。合成的纳米材料具有很高的化学电阻响应,检测极限超过 ppm 级。当传感器数量较少时,特定的材料组合被证明具有相关性,然而,随着传感器数量的增加,这种重要性就会降低。我们的研究表明,芯片上的材料组合可以提高对特定分析物的选择性,而忽略其他分析物。因此,在组合分析的支持下,现代合成方法和打印协议可能会为制造片上正交多传感器系统铺平道路。
{"title":"Multioxide combinatorial libraries: fusing synthetic approaches and additive technologies for highly orthogonal electronic noses","authors":"Vishalkumar Rajeshbhai Gohel, Margarita Chetyrkina, Andrey Gaev, Nikolay P. Simonenko, Tatiana L Simonenko, Philipp Yu Gorobtsov, Nikita Fisenko, Darya A. Dudorova, Valeriy Zaytsev, Anna Lantsberg, Elizaveta P. Simonenko, Albert G. Nasibulin, Fedor Fedorov","doi":"10.1039/d4lc00252k","DOIUrl":"https://doi.org/10.1039/d4lc00252k","url":null,"abstract":"This study evaluates the performance advancement of electronic noses, on-chip engineered multisensor systems, exploiting a combinatorial approach. We analyze a spectrum of metal oxide semiconductor materials produced by individual methods of liquid-phase synthesis and a combination of chemical deposition, and sol-gel method, with hydrothermal treatment. These methodologies are demonstrated to enable obtaining a fairly wide range of nanomaterials that differ significantly in chemical composition, crystal structure, and morphological features. While synthesis routes foster diversity in materials properties, microplotter printing ensures targeted precision in making on-chip arrays for evaluation of combinatorial selectivity concept in the task of organic vapors, like alcohol homologs, acetone, and benzene, classification. The synthesized nanomaterials demonstrate a high chemiresistive response, with limit of detection beyond a ppm level. A specific combination of materials is demonstrated to be relevant when the number of sensors is low, however, such importance diminishes with an increase in the sensor number. We show that on-chip material combinations could favor selectivity to a specific analyte, disregarding the others. Hence, modern synthesis methods, and printing protocols supported by combinatorial analysis might pave the way for fabricating on-the-chip orthogonal multisensor systems.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141556741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A centrifugal-driven spiral microchannel microfiltration chip for emulsion and deformable particle sorting. 用于乳液和可变形颗粒分选的离心驱动螺旋微通道微过滤芯片。
IF 6.1 2区 工程技术 Q1 BIOCHEMICAL RESEARCH METHODS Pub Date : 2024-07-04 DOI: 10.1039/d4lc00260a
Yongchao Cai, Zekun Li, Cuimin Sun, Xuan Zhao, Shixiong Wu, Guangyong Huang, shengchang Tang, Peng Dai, Xiangfu Wei, Hui You
Droplet sorting and enrichment, as a prominent field within microfluidic technology, represent a pivotal stage in the manipulation of droplets and particles. In recent times, droplet sorting methods based on lab-on-disk (LOD) have garnered significant interest among researchers for their inherent merits, including high throughput, ease of operation, seamless device integration, and independence from supplementary driving forces. This study introduces a centrifugal force-driven microfluidic chip comprising spiral microchannels. The chip incorporates microhole arrays along the sidewall of the spiral channels, enabling size-based sorting and enrichment of microdroplets under the influence of multiple forces. Firstly, a comparative analysis was performed to assess the influence of the separation port structure and rotational speed on efficiency, and a mechanical modeling approach was employed to conduct kinetic analyses of droplet behavior during instantaneous separation. Those findings demonstrated a good agreement with the experimental results at ω <100 rpm. Subsequently, sorting experiments on homogeneous droplets indicated that repetitive sorting could increase the recovery ratios, RT(α), of high-concentration droplets (20.7%) from 35.3% to over 80%. We also conducted a sorting experiment on three-component homogeneous-phase emulsions using a serially connected chip array, and the sorting throughput was 0.58 mL/min. As a result, the RT(α) for 60 and 160 μm droplets were 99.4% and 88.9%, respectively. Lastly, we conducted elution experiments and dualsample sorting on a single chip, and the fluorescence results demonstrated that this study provided an efficient and non-cross-contaminating sorting method for non-homogenous phase multi-sample microreactor units.
液滴分选和富集是微流体技术中的一个重要领域,代表着液滴和颗粒操作的一个关键阶段。近来,基于盘上实验室(LOD)的液滴分选方法因其固有的优点,包括高通量、易操作、无缝设备集成以及不受辅助驱动力影响等,引起了研究人员的极大兴趣。本研究介绍了一种由螺旋微通道组成的离心力驱动微流控芯片。该芯片沿螺旋通道的侧壁集成了微孔阵列,可在多种力的作用下实现基于尺寸的微滴分拣和富集。首先进行了比较分析,以评估分离口结构和转速对效率的影响,并采用机械建模方法对瞬时分离过程中的液滴行为进行动力学分析。这些研究结果表明与 ω <100 rpm 时的实验结果非常吻合。随后,对均匀液滴进行的分拣实验表明,重复分拣可将高浓度液滴(20.7%)的回收率 RT(α) 从 35.3% 提高到 80% 以上。我们还利用串联芯片阵列对三组分均相乳液进行了分拣实验,分拣吞吐量为 0.58 mL/min。结果,60 和 160 μm 液滴的 RT(α) 分别为 99.4% 和 88.9%。最后,我们在单个芯片上进行了洗脱实验和双样品分选,荧光结果表明该研究为非均相多样品微反应器单元提供了一种高效、无交叉污染的分选方法。
{"title":"A centrifugal-driven spiral microchannel microfiltration chip for emulsion and deformable particle sorting.","authors":"Yongchao Cai, Zekun Li, Cuimin Sun, Xuan Zhao, Shixiong Wu, Guangyong Huang, shengchang Tang, Peng Dai, Xiangfu Wei, Hui You","doi":"10.1039/d4lc00260a","DOIUrl":"https://doi.org/10.1039/d4lc00260a","url":null,"abstract":"Droplet sorting and enrichment, as a prominent field within microfluidic technology, represent a pivotal stage in the manipulation of droplets and particles. In recent times, droplet sorting methods based on lab-on-disk (LOD) have garnered significant interest among researchers for their inherent merits, including high throughput, ease of operation, seamless device integration, and independence from supplementary driving forces. This study introduces a centrifugal force-driven microfluidic chip comprising spiral microchannels. The chip incorporates microhole arrays along the sidewall of the spiral channels, enabling size-based sorting and enrichment of microdroplets under the influence of multiple forces. Firstly, a comparative analysis was performed to assess the influence of the separation port structure and rotational speed on efficiency, and a mechanical modeling approach was employed to conduct kinetic analyses of droplet behavior during instantaneous separation. Those findings demonstrated a good agreement with the experimental results at ω &lt;100 rpm. Subsequently, sorting experiments on homogeneous droplets indicated that repetitive sorting could increase the recovery ratios, RT(α), of high-concentration droplets (20.7%) from 35.3% to over 80%. We also conducted a sorting experiment on three-component homogeneous-phase emulsions using a serially connected chip array, and the sorting throughput was 0.58 mL/min. As a result, the RT(α) for 60 and 160 μm droplets were 99.4% and 88.9%, respectively. Lastly, we conducted elution experiments and dualsample sorting on a single chip, and the fluorescence results demonstrated that this study provided an efficient and non-cross-contaminating sorting method for non-homogenous phase multi-sample microreactor units.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141546238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Modelling the innate immune system in microphysiological systems. 在微物理系统中模拟先天性免疫系统。
IF 6.1 2区 工程技术 Q1 BIOCHEMICAL RESEARCH METHODS Pub Date : 2024-07-03 DOI: 10.1039/d3lc00812f
Michael J Rupar, Hannah Hanson, Stephanie Rogers, Brianna Botlick, Steven Trimmer, James J Hickman

This critical review aims to highlight how modeling of the immune response has adapted over time to utilize microphysiological systems. Topics covered here will discuss the integral components of the immune system in various human body systems, and how these interactions are modeled using these systems. Through the use of microphysiological systems, we have not only expanded on foundations of basic immune cell information, but have also gleaned insight on how immune cells work both independently and collaboratively within an entire human body system.

这篇评论旨在强调免疫反应建模如何随着时间的推移而适应利用微生理系统。这里涉及的主题将讨论人体各系统中免疫系统不可或缺的组成部分,以及如何利用这些系统对这些相互作用进行建模。通过使用微观生理学系统,我们不仅扩展了免疫细胞基本信息的基础,还深入了解了免疫细胞如何在整个人体系统中独立和协同工作。
{"title":"Modelling the innate immune system in microphysiological systems.","authors":"Michael J Rupar, Hannah Hanson, Stephanie Rogers, Brianna Botlick, Steven Trimmer, James J Hickman","doi":"10.1039/d3lc00812f","DOIUrl":"https://doi.org/10.1039/d3lc00812f","url":null,"abstract":"<p><p>This critical review aims to highlight how modeling of the immune response has adapted over time to utilize microphysiological systems. Topics covered here will discuss the integral components of the immune system in various human body systems, and how these interactions are modeled using these systems. Through the use of microphysiological systems, we have not only expanded on foundations of basic immune cell information, but have also gleaned insight on how immune cells work both independently and collaboratively within an entire human body system.</p>","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141490046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Microfluidic finger-actuated mixer for ultrasensitive electrochemical measurements of protein biomarkers for point-of-care testing 用于超灵敏电化学测量蛋白质生物标记物的微流控指动混合器,可用于床旁检测
IF 6.1 2区 工程技术 Q1 BIOCHEMICAL RESEARCH METHODS Pub Date : 2024-07-02 DOI: 10.1039/d4lc00207e
Benjamin Utzinger, Desh Deepak Dixit, Peter B. Lillehoj
Current diagnostic tests for high sensitivity detection of protein biomarkers involve long incubation times or require bulky/expensive instrumentation, hindering their use for point-of-care testing. Here, we report a microfluidic electrochemical immunosensor that employs a unique finger-actuated mixer for rapid, ultrasensitive measurements of protein biomarkers. Mixing was implemented during the incubation steps, which accelerated biomolecular transport and promoted immunocomplex formation, leading to enhanced analytical sensitivity and a shortened detection time. Electrochemical measurements were performed using a handheld diagnostic device consisting of a smartphone and miniature potentiostat. Proof of principle was demonstrated by using this platform for quantitative measurements of C-X-C motif chemokine ligand 9 (CXCL9), a serological biomarker for autoimmune and inflammatory diseases, which could be detected in human plasma at concentrations as low as 4.7 pg mL-1 in < 25 min. The ability to rapidly detect protein biomarkers with high sensitivity in a point-of-care format makes this device a promising tool for diagnostic testing, particularly in resource-limited settings.
目前用于高灵敏度检测蛋白质生物标记物的诊断测试需要较长的孵育时间,或需要笨重/昂贵的仪器,这阻碍了它们在护理点测试中的应用。在这里,我们报告了一种微流控电化学免疫传感器,它采用了独特的指动混合器,可快速、超灵敏地测量蛋白质生物标记物。在孵育步骤中进行混合,可加速生物分子运输并促进免疫复合物的形成,从而提高分析灵敏度并缩短检测时间。电化学测量是使用由智能手机和微型恒电位仪组成的手持诊断设备进行的。利用该平台对自身免疫性疾病和炎症性疾病的血清生物标志物 C-X-C motif 趋化因子配体 9 (CXCL9)进行了定量测量,证明了其原理,在 < 25 分钟内可检测到人体血浆中低至 4.7 pg mL-1 的浓度。该设备能以护理点的形式快速、高灵敏度地检测蛋白质生物标记物,因此是一种很有前景的诊断检测工具,尤其是在资源有限的环境中。
{"title":"Microfluidic finger-actuated mixer for ultrasensitive electrochemical measurements of protein biomarkers for point-of-care testing","authors":"Benjamin Utzinger, Desh Deepak Dixit, Peter B. Lillehoj","doi":"10.1039/d4lc00207e","DOIUrl":"https://doi.org/10.1039/d4lc00207e","url":null,"abstract":"Current diagnostic tests for high sensitivity detection of protein biomarkers involve long incubation times or require bulky/expensive instrumentation, hindering their use for point-of-care testing. Here, we report a microfluidic electrochemical immunosensor that employs a unique finger-actuated mixer for rapid, ultrasensitive measurements of protein biomarkers. Mixing was implemented during the incubation steps, which accelerated biomolecular transport and promoted immunocomplex formation, leading to enhanced analytical sensitivity and a shortened detection time. Electrochemical measurements were performed using a handheld diagnostic device consisting of a smartphone and miniature potentiostat. Proof of principle was demonstrated by using this platform for quantitative measurements of C-X-C motif chemokine ligand 9 (CXCL9), a serological biomarker for autoimmune and inflammatory diseases, which could be detected in human plasma at concentrations as low as 4.7 pg mL-1 in &lt; 25 min. The ability to rapidly detect protein biomarkers with high sensitivity in a point-of-care format makes this device a promising tool for diagnostic testing, particularly in resource-limited settings.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141495611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Microreactor designed for efficient plasma-liquid segmented flows 专为高效等离子体-液体分段流设计的微反应器
IF 6.1 2区 工程技术 Q1 BIOCHEMICAL RESEARCH METHODS Pub Date : 2024-07-02 DOI: 10.1039/d4lc00315b
Pierre Dedieu, Gabriel Morand, Karine Loubière, Stéphanie Ognier, Michael Tatoulian
Microreactors were designed for gas-liquid plasma chemical processes and operated under segmented flows in a high aspect ratio (8.76) rectangular microchannel. First, the hydrodynamics of the gas-liquid flows generated at a T-junction was investigated for fifteen solvents commonly used in organic synthesis. The classical literature scaling laws were revised to describe the dependence of bubble and slug lengths, and bubble residence time on the liquid nature by introducing their liquid vapour pressure. Liquid film thickness and liquid residence time were estimated from residence time distribution experiments. Secondly, plasma could be successfully generated in these segmented flows for all the liquids. Due to the plasma dissipation of thermal energy, gas phase temperature increased and induced the lengthening of bubbles and the decrease in bubble residence time. Flow pattern was also impacted by the gas temperature increase. A flow map describing the evolution of flow pattern under plasma conditions was built, enabling prediction of the flow pattern based on liquid boiling point and dielectric constant. These microreactors have demonstrated great potential, and by adapting the synthesis solvent or the operating plasma conditions, they could find promising applications in gas-liquid plasma chemical processes.
设计了用于气液等离子化学过程的微反应器,并在高纵横比(8.76)矩形微通道中的分段流下运行。首先,针对有机合成中常用的 15 种溶剂,研究了 T 型交界处产生的气液流的流体力学。通过引入液体蒸汽压,对经典文献的缩放定律进行了修订,以描述气泡和蛞蝓长度以及气泡停留时间对液体性质的依赖性。根据停留时间分布实验估算了液膜厚度和液体停留时间。其次,所有液体都能在这些分段流中成功产生等离子体。由于等离子体耗散热能,气相温度升高,导致气泡延长,气泡停留时间缩短。流动模式也受到气体温度升高的影响。我们绘制了等离子条件下流动模式演变的流动图,从而能够根据液体沸点和介电常数预测流动模式。这些微反应器显示出巨大的潜力,通过调整合成溶剂或操作等离子条件,它们在气液等离子化学过程中的应用前景广阔。
{"title":"Microreactor designed for efficient plasma-liquid segmented flows","authors":"Pierre Dedieu, Gabriel Morand, Karine Loubière, Stéphanie Ognier, Michael Tatoulian","doi":"10.1039/d4lc00315b","DOIUrl":"https://doi.org/10.1039/d4lc00315b","url":null,"abstract":"Microreactors were designed for gas-liquid plasma chemical processes and operated under segmented flows in a high aspect ratio (8.76) rectangular microchannel. First, the hydrodynamics of the gas-liquid flows generated at a T-junction was investigated for fifteen solvents commonly used in organic synthesis. The classical literature scaling laws were revised to describe the dependence of bubble and slug lengths, and bubble residence time on the liquid nature by introducing their liquid vapour pressure. Liquid film thickness and liquid residence time were estimated from residence time distribution experiments. Secondly, plasma could be successfully generated in these segmented flows for all the liquids. Due to the plasma dissipation of thermal energy, gas phase temperature increased and induced the lengthening of bubbles and the decrease in bubble residence time. Flow pattern was also impacted by the gas temperature increase. A flow map describing the evolution of flow pattern under plasma conditions was built, enabling prediction of the flow pattern based on liquid boiling point and dielectric constant. These microreactors have demonstrated great potential, and by adapting the synthesis solvent or the operating plasma conditions, they could find promising applications in gas-liquid plasma chemical processes.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141489489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Sample preparation and detection methods in point-of-care devices towards future at-home testing. 面向未来家庭检测的护理点设备中的样品制备和检测方法。
IF 6.1 2区 工程技术 Q1 BIOCHEMICAL RESEARCH METHODS Pub Date : 2024-07-02 DOI: 10.1039/d3lc00943b
George Adedokun, Morteza Alipanah, Z Hugh Fan

Timely and accurate diagnosis is critical for effective healthcare, yet nearly half the global population lacks access to basic diagnostics. Point-of-care (POC) testing offers partial solutions by enabling low-cost, rapid diagnosis at the patient's location. At-home POC devices have the potential to advance preventive care and early disease detection. Nevertheless, effective sample preparation and detection methods are essential for accurate results. This review surveys recent advances in sample preparation and detection methods at POC. The goal is to provide an in-depth understanding of how these technologies can enhance at-home POC devices. Lateral flow assays, nucleic acid tests, and virus detection methods are at the forefront of POC diagnostic technology, offering rapid and sensitive tools for identifying and measuring pathogens, biomarkers, and viral infections. By illuminating cutting-edge research on assay development for POC diagnostics, this review aims to accelerate progress towards widely available, user-friendly, at-home health monitoring tools that empower individuals in personalized healthcare in the future.

及时、准确的诊断对有效的医疗保健至关重要,然而全球近一半人口无法获得基本的诊断服务。护理点(POC)检测提供了部分解决方案,可在患者所在地进行低成本的快速诊断。家用 POC 设备具有推进预防保健和早期疾病检测的潜力。然而,有效的样本制备和检测方法对获得准确的结果至关重要。本综述探讨了 POC 样品制备和检测方法的最新进展。目的是深入了解这些技术如何增强家用 POC 设备。侧流检测、核酸测试和病毒检测方法是 POC 诊断技术的前沿,为识别和测量病原体、生物标记物和病毒感染提供了快速、灵敏的工具。本综述介绍了 POC 诊断化验开发方面的前沿研究,旨在加快普及用户友好型居家健康监测工具的进程,使个人在未来能够获得个性化的医疗保健服务。
{"title":"Sample preparation and detection methods in point-of-care devices towards future at-home testing.","authors":"George Adedokun, Morteza Alipanah, Z Hugh Fan","doi":"10.1039/d3lc00943b","DOIUrl":"https://doi.org/10.1039/d3lc00943b","url":null,"abstract":"<p><p>Timely and accurate diagnosis is critical for effective healthcare, yet nearly half the global population lacks access to basic diagnostics. Point-of-care (POC) testing offers partial solutions by enabling low-cost, rapid diagnosis at the patient's location. At-home POC devices have the potential to advance preventive care and early disease detection. Nevertheless, effective sample preparation and detection methods are essential for accurate results. This review surveys recent advances in sample preparation and detection methods at POC. The goal is to provide an in-depth understanding of how these technologies can enhance at-home POC devices. Lateral flow assays, nucleic acid tests, and virus detection methods are at the forefront of POC diagnostic technology, offering rapid and sensitive tools for identifying and measuring pathogens, biomarkers, and viral infections. By illuminating cutting-edge research on assay development for POC diagnostics, this review aims to accelerate progress towards widely available, user-friendly, at-home health monitoring tools that empower individuals in personalized healthcare in the future.</p>","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141475477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Deformation under flow and morphological recovery of cancer cells 癌细胞的流动变形和形态恢复
IF 6.1 2区 工程技术 Q1 BIOCHEMICAL RESEARCH METHODS Pub Date : 2024-07-01 DOI: 10.1039/d4lc00246f
Emile Gasser, Emilie Su, Kotryna Vaidžiulytė, Nassiba Abbade, Hamizah Cognart, Jean-Baptiste Manneville, Jean-Louis Viovy, Matthieu Piel, Jean-Yves Pierga, Kyohei Terao, Catherine Villard
The metastatic cascade includes a blood circulation step for cells detached from the primary tumor. This stage involves significant shear stress as well as large and fast deformations as the cells circulate through the microvasculature. These mechanical stimuli are well reproduced in microfluidic devices. However, the recovery dynamics after deformation is also pivotal to understand how a cell can pass through the multiple capillary constrictions encountered during a single hemodynamic cycle. The microfluidic system developed in this work allows to study single cell recovery in flow-free conditions following pressure-actuated cell deformation inside constricted microchannels. We used three breast cancer cell lines - namely MCF-7, SK-BR3 and MDA-MB231 - as cellular models representative of different cancer phenotypes. Changing the size of the constriction allows to explore moderate to strong deformation regimes, the latter being associated to the formation of plasma membrane blebs. In the regime of moderate deformation, all cell types display a fast elastic recovery behavior followed by a slower viscoelastic regime, well described by a double exponential decay. Among the three cell types, cells of the mesenchymal phenotype, i.e. the MDA-MB231 cells, are softer and the most fluid-like, in agreement with previous studies. Our main finding here is that the fast elastic recovery regime revealed by our novel microfluidic system is under the control of cell contractility ensured by the integrity of the cell cortex. Our results suggest that the cell cortex plays a major role during the transit of circulating tumor cells by allowing their fast morphological recovery after deformation in blood capillaries.
转移过程包括脱离原发肿瘤的细胞的血液循环步骤。在这一阶段,细胞在微血管中循环时会产生巨大的剪切应力以及巨大而快速的变形。微流控设备可以很好地再现这些机械刺激。然而,变形后的恢复动态也是了解细胞如何通过单个血液动力学周期中遇到的多个毛细血管收缩的关键。这项工作中开发的微流体系统可在无流条件下研究收缩微通道内压力作用下细胞变形后的单细胞恢复情况。我们使用了三种乳腺癌细胞系--即 MCF-7、SK-BR3 和 MDA-MB231,作为代表不同癌症表型的细胞模型。通过改变收缩的大小,可以探索从中等到强烈的变形机制,后者与质膜出血点的形成有关。在中度变形机制中,所有类型的细胞都表现出快速弹性恢复行为,随后是较慢的粘弹性机制,这可以用双指数衰减很好地描述。在这三种细胞类型中,间充质表型细胞(即 MDA-MB231 细胞)更柔软,最像流体,这与之前的研究一致。我们在此的主要发现是,我们的新型微流控系统所揭示的快速弹性恢复机制是由细胞皮层的完整性所确保的细胞收缩性控制的。我们的研究结果表明,细胞皮层在循环肿瘤细胞的转运过程中发挥了重要作用,使其在毛细血管中变形后能够快速恢复形态。
{"title":"Deformation under flow and morphological recovery of cancer cells","authors":"Emile Gasser, Emilie Su, Kotryna Vaidžiulytė, Nassiba Abbade, Hamizah Cognart, Jean-Baptiste Manneville, Jean-Louis Viovy, Matthieu Piel, Jean-Yves Pierga, Kyohei Terao, Catherine Villard","doi":"10.1039/d4lc00246f","DOIUrl":"https://doi.org/10.1039/d4lc00246f","url":null,"abstract":"The metastatic cascade includes a blood circulation step for cells detached from the primary tumor. This stage involves significant shear stress as well as large and fast deformations as the cells circulate through the microvasculature. These mechanical stimuli are well reproduced in microfluidic devices. However, the recovery dynamics after deformation is also pivotal to understand how a cell can pass through the multiple capillary constrictions encountered during a single hemodynamic cycle. The microfluidic system developed in this work allows to study single cell recovery in flow-free conditions following pressure-actuated cell deformation inside constricted microchannels. We used three breast cancer cell lines - namely MCF-7, SK-BR3 and MDA-MB231 - as cellular models representative of different cancer phenotypes. Changing the size of the constriction allows to explore moderate to strong deformation regimes, the latter being associated to the formation of plasma membrane blebs. In the regime of moderate deformation, all cell types display a fast elastic recovery behavior followed by a slower viscoelastic regime, well described by a double exponential decay. Among the three cell types, cells of the mesenchymal phenotype, i.e. the MDA-MB231 cells, are softer and the most fluid-like, in agreement with previous studies. Our main finding here is that the fast elastic recovery regime revealed by our novel microfluidic system is under the control of cell contractility ensured by the integrity of the cell cortex. Our results suggest that the cell cortex plays a major role during the transit of circulating tumor cells by allowing their fast morphological recovery after deformation in blood capillaries.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141475185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
Lab on a Chip
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1