ACS Applied Bio Materials最新文献

The development of simple and versatile approaches for the fabrication of DNA-based composite nanomaterials, endowed with defined morphologies and specific functionalities, is of paramount importance for various applications. Herein, we report a simple approach for the synthesis of multifunctional copper-DNA nanoflowers (Cu-DNF) that exclusively consist of rolling circle polymerized nanoflowers (DNF) and in situ synthesized concatemeric fluorescence copper nanoparticles. Through meticulous regulation of the assembly process, it is possible to generate Cu-DNF with precise sizes and stable fluorescence properties. The obtained Cu-DNF possesses robust biostability to resist degradation by nuclease, presumably resulting from the dense structure of the Cu-DNF. The Cu-DNF were also encoded with polyvalent tandem CD63 aptamer sequences, which enhanced their binding affinity and internalization efficiency into tumor cells. We demonstrate that the multifunctional Cu-DNF can efficiently internalize tumor cells for tracking and imaging analysis of intracellular microRNA. This approach may be beneficial for creating multifunctional DNA-based composite nanomaterials for various technological applications.

Corneal lenticules can be deposited and retrieved for vision-restoring surgeries. Extended transportation logistical delays from the lenticule bank to the clinic could be a concern. To investigate this, corneal lenticules were cryopreserved at a Ministry of Health of Singapore-licensed lenticule bank for 1 year and were then transported at 4 °C. The transparency was measured daily until significant degradation was notable, compared to fresh lenticules from donor corneas (n = 3). The molecular and ultrastructural integrity of lenticules after 1 day in transport (n = 3) and on the day of transparency deterioration (n = 3) was evaluated by histochemistry and transmission electron microscopy (TEM). In addition, 9 rabbits were implanted with these lenticules to assess the difference in postoperative outcomes by corneal imaging, immunofluorescence staining, and TEM. Lenticules showed a significant transparency reduction after 6 days in transit (p = 0.002). The change was not caused by collagen, glycosaminoglycan, and glycoprotein alterations but by a significantly greater shift in the distribution of the interfibrillar distance (IFD) (Z = 4.419; p < 0.001) and fibrillar diameter (FD) (Z = 6.435; p < 0.001). When implanted, day 6 lenticules exhibited greater light reflectivity and slower recovery of clarity compared to fresher lenticules, despite a combination of corneal imaging and immunofluorescence staining showing no fibrosis, inflammation, or vascularization in either group. With TEM, the discrepancy was revealed due to the difference in the restoration of IFD and FD distribution. In conclusion, banked lenticules can maintain transparency for up to 5 days of transportation at 4 °C. Further delays compromise their ultrastructural integrity and postoperative clarity, emphasizing the need to factor in transportation in lenticule banking logistics.

This research aimed to evaluate the potency of preparation based on heparinized iron oxide nanoparticles (hIONPs) in combination with radiation therapy, including magnetic delivery via the applied magnetic field (AMF), in sarcoma and cervical cancer models. For in vitro studies, cells of rhabdomyosarcoma (RD), fibrosarcoma (HT1080), and cervical cancer (HeLa S3) were treated with hIONPs and analyzed for survival rate and hIONP uptake. Then, cell morphology, cell cycle, increase of reactive oxygen species, mitochondria depolarization, and ability to form colonies were assessed for combined treatment (hIONPs + 3Gy). For in vivo research, hIONPs were administered once in the hybrids of CBAxC57Bl/6j mice, grafted with sarcoma (S37) and cervical cancer (CC5) strains. The ultimate in vivo treatment modes were: (1) i.v. hIONPs (14 μg/kg) + 5 Gy; (2) i.v. hIONPs (14 μg/kg) + AMF + 5 Gy; and (3) i.t. hIONPs (2,8 μg/kg) + 5 Gy. The overall survival rates, increase in life expectancy, inhibition of tumor growth (tumor growth inhibition), and degree of inhibition (T/C) were determined, and pathomorphological changes were assessed in experimental groups. The combined treatment in vitro (hIONPs + 3Gy) promotes multiple tumor cell death with high-severity peroxide effects compared with other groups. The sarcoma cells were more sensitive than cervical cancer cells. For in vivo, an enhancing effect was revealed by the combination of radiotherapy and magnetic-delivered hIONPs. For S37 tumor, the treatment regimen was characterized as having a high antitumor effect, ≪++++ ≫, with a 20% cure rate of mice. For the CC5 tumor, the effect was accompanied by the inhibition of tumor growth, an increase in the life expectancy of animals, and was characterized as a significant antitumor effect, ≪+++/++ ≫. From the data obtained, it can be concluded that the radiosensitizing potential of hIONPs may be taken as a basis of combined radiation treatment protocols.

With the recent upsurge of data-driven technology, the demand for storage elements has pushed the researchers to explore design of nobel nonvolatile memory devices with diverse functionalities. However, the management of electronic waste has become a prominent challenge due to the rapid growth of the solid-state electronics industry. Biomaterial-based Resistive Random Access Memory (Bio-RRAM) has become one of the most promising devices that can augment the quality of memory devices because of their environmentally benign behavior, biocompatible, nontoxic, transient, transferable, flexible, dissolvable, and biodegradable nature. In this work, we report the fabrication of MIM-structured RRAM devices based on two biomaterials, namely, ovalbumin liquid and acemannan polysaccharide gel, as switching layers. Further, they are characterized by several analytical techniques. The electrical transport measurement revealed bipolar resistive switching behavior, sustainable over 1000 consecutive cycles. The devices demonstrated supreme endurance over 1000 switching cycles with a maximum ON/OFF ratio of ∼10²-10³. The switching process can be explained through the formation and rupture of conducting filaments formed by the migration of Ag ions. Design of neuro-memristive synapse has been further been explored to demonstrate various neuromorphic functionalities such as long/short-term potentiation, depression, and plasticity. Due to simultaneous presence of resistive switching with the negative differential resiatance (NDR) effect, remarkable endurance, ease of fabrication, cost reduction, and environmental compatibility, neuromorphic functionalities, the RRAM structures could be of potential interest for bioelectronic memory design, wearable and flexible electronics and neuromorphic computing.

In this study, we initially assessed the antioxidant activities of various HG-type hawthorn pectin-iron(III) complexes, which were synthesized in our previous research, utilizing methods such as reducing assay, 2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and scavenging, and hydroxyl radical scavenging. The results showed that the antioxidant capacity of the hawthorn pectin iron(III) complexes exhibited a positive correlation with increasing concentrations, ranging from 0.2 to 1.0 mg/mL. Notably, the pectin complex chelated with iron via ultrasonic extraction and de-esterification for 20 min (designated as DU20-Fe) showed the most significant antioxidant activity. Subsequently, DU20-Fe was chosen for in vivo activity assessment in a mouse model of iron-deficiency anemia (IDA). The findings indicated that DU20-Fe significantly enhanced levels of red blood cells (RBC), mean corpuscular volume (MCV), and mean corpuscular hemoglobin (MCH) content in the mice with IDA. Furthermore, histological analysis of the liver demonstrated that DU20-Fe effectively alleviated liver damage in the IDA mice. These results validate the potential of hawthorn pectin-iron complexes in exhibiting anti-iron-deficiency anemia activity, suggesting that the hawthorn pectin-iron(III) complex may be developed into a bio-polysaccharide-based iron supplement with multiple health benefits.

Bone defects arising from trauma, disease, or surgical intervention represent significant challenges. Developing effective bone tissue engineering strategies to address these issues and promote repair is crucial. β-Tricalcium phosphate (β-TCP) has emerged as a promising synthetic graft due to its porous, degradable structure and excellent biocompatibility. However, the lack of biological cues in β-TCP limits its functionality, requiring different surface modification strategies. Bone morphogenetic protein-2 mimetic peptide (BMP; NSVNSKIPKACCVPTELSAI) and collagen mimetic peptide (CMP; GTPGPQGIAGQRGVV) have a known significant therapeutic potential due to their ability to enhance cell attachment and osteogenic differentiation. Herein, a peptide functionalization strategy for β-TCP scaffolds was introduced. Briefly, β-TCP was treated with cold atmospheric plasma (CAP) to create functional hydroxyl groups on the surface of the β-TCP. Subsequently, peptides were conjugated by using a three-step method: (1) silanization with APTES, (2) EDC activation, and (3) peptide conjugation. The successful surface modification with CAP and peptide conjugation was confirmed via XRD, FTIR, and Raman analysis. Furthermore, the effects of BMP and CMP peptides on osteogenic differentiation after CAP treatment were investigated in human mesenchymal stem cells (hMSCs). Both β-TCP/BMP and β-TCP/CMP scaffolds demonstrated excellent biocompatibility with hMSCs, enhancing cell proliferation and promoting osteogenic differentiation. Remarkably, β-TCP/CMP showed better results in terms of proliferation and differentiation compared with β-TCP/BMP. These findings highlight the clinical potential of peptide-functionalized β-TCP scaffolds for bone tissue engineering while also providing a promising methodology for β-TCP functionalization.

Objective: To compare masticatory muscle activity between people with cystic fibrosis (pwCF) and healthy controls and to verify whether craniocervical dysfunction is associated with the presence of CF.

Methods: Fifty-six participants were assessed and divided into pwCF and healthy control (HC) groups, each one composed of 13 children and adolescents at 9 (SD 3) years old and 15 adults at 25 (SD 6) years old. Craniocervical Dysfunction Index assessed symptoms of dysfunction and cervical spine mobility. Electromyography was used to evaluate the jaw and neck muscle activity during chewing.

Results: Muscle activity during chewing was not statistically different between groups. Prevalence of craniocervical dysfunction was 75% for pwCF vs 64% for healthy controls. Individuals with CF are 1.53 [1.260, 1.870] times more likely to have reduced cervical mobility compared to healthy controls (p = 0.000).

Conclusion: These results reinforce the need for musculoskeletal disorders treatment in the management of pwCF.

Objective: To compare the temporomandibular joint (TMJ) position and shape between skeletal Class I subjects with different degrees of deep overbite or open bite and unaffected peers.

Methods: Cone beam computed tomography (CBCT) images of 90 participants, equally divided into deep bite, open bite, and control groups (DBG, OBG, and CG, respectively), were analyzed. The DBG and OBG were further subdivided into three subgroups based on the severity. Linear and angular measurements were used to determine the positional and morphological characteristics of the TMJ.

Results: DBG showed significantly larger superior joint space, anterior inclination of the condyle, and articular eminence inclination than CG and OBG. The severity of deep bite and open bite significantly influenced the TMJ position and morphology.

Conclusion: In severe deep overbite and open bite cases, there were considerable alterations in TMJ components, most likely as a result of functional demands.

Objective: To identify the relationship between the use of selective serotonin reuptake inhibitors (SSRIs) as pharmacological treatment and the development of bruxism.

Methods: A systematic literature search was performed in PubMed and Scopus databases, which included articles written within the last 30 years. The working hypothesis was that there is a significant association between using SSRIs as an antidepressant medication and developing bruxism.

Results: A total of four articles were included in the systematic review. All the articles are related to SSRI antidepressants and bruxism.

Conclusion: The results indicate that there is an apparent association between SSRI drug treatment and the development of bruxism. The methods of evaluation and analysis were different in each article and cannot be considered conclusive; however, there is sufficient information to elucidate the impact of SSRI drugs on the development of bruxism.

Background: Pterygoid hamulus syndrome, a painful oral and facial syndrome, has been described in literature to be correlated with morphological changes in the length of the pterygoid hamulus of the sphenoid bone.

Clinical presentation: The current case report describes the treatment for severe, continuous pain in the posterior right palate. Despite numerous conservative treatments given to the patient, no improvement was seen. Cone beam computed tomography (CBCT) measurements revealed an elongation as well as a significant medial deviation of the lower extremity of the medial pterygoid plate. The surgical resection was performed under local anesthesia. The pain subsided two days after the surgery, and there were no relapses in the weeks that followed.

Conclusion: The medial deviation of the hamulus appeared to be important in the etiology of this painful syndrome. Additional research based on CBCT measurements will be required.