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β-Cyclodextrin-Tethered Butein, a Greener Redox-Active Biomaterial for Electrochemical Enzymatic Sensing of Sialic Acid. β-Cyclodextrin-Tethered Butein--一种更绿色的氧化还原活性生物材料,可用于硅酸的电化学酶传感。
IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS Pub Date : 2024-07-15 Epub Date: 2024-06-13 DOI: 10.1021/acsabm.4c00474
Ramya Kanagaraj, Vinoth Krishnan, Shanmugam Senthil Kumar, Murugan Veerapandian

Biocompatible, industrially scalable, and opto/electrochemically active biomaterials are promising for biosensor platform design and application. Herein, cyclic oligosaccharide, β-cyclodextrin (BCD), is conjugated with Butein, a chalcone-type polyphenol, via dehydration reaction of the hydroxyl groups of BCD and the benzoyl ring of Butein. Functional group changes in the conjugated BCD-Butein were comprehensively studied using UV-visible absorbance, Fourier transform-infrared, and X-ray photoelectron spectroscopic techniques. The electrochemical characteristics of BCD-Butein were explored using cyclic voltammetry, showing the reversible redox behavior (2e-/2H+) attributed to the catecholic OH group of Butein. The BCD-Butein-modified electrode exhibits a surface-confined redox process (R2 = 0.99, Ipa and Ipc) at the interface, suitable for external mediatorless sensor studies. An enzymatic biomolecular sensor has been constructed using BCD-Butein-modified glassy carbon and a screen-printed electrode targeting sialic acid as the model clinical biomarker. With the enzyme sialic acid aldolase, BCD-Butein-modified substrate exhibited a selective conversion of sialic acid to N-acetyl-d-mannosamine and pyruvate, with a wide linear detection range (1-100 nM), the lowest detection limit of 0.2 nM, and a quantification limit of 0.69 nM, convenient for clinical threshold diagnosis.

具有生物兼容性、工业可扩展性和光电化学活性的生物材料在生物传感器平台的设计和应用中大有可为。本文通过 BCD 的羟基和 Butein 的苯甲酰基环的脱水反应,将环状低聚糖 β-环糊精(BCD)与查尔酮类多酚 Butein 共轭。利用紫外-可见吸收光谱、傅立叶变换红外光谱和 X 射线光电子能谱技术对共轭 BCD-Butein 的官能团变化进行了全面研究。利用循环伏安法研究了 BCD-芸香素的电化学特性,结果表明芸香素的儿茶酚羟基具有可逆的氧化还原行为(2e-/2H+)。经 BCD-Butein 修饰的电极在界面上表现出表面封闭的氧化还原过程(R2 = 0.99,Ipa 和 Ipc),适合于外部无介质传感器研究。利用 BCD-Butein 改性玻璃碳和丝网印刷电极构建了一种酶生物分子传感器,目标是作为临床生物标记物模型的硫辛酸。在乙酰丙酸醛缩酶的作用下,BCD-Butein修饰的底物可将乙酰丙酸选择性地转化为N-乙酰-d-甘露胺和丙酮酸,其线性检测范围宽(1-100 nM),最低检测限为0.2 nM,定量限为0.69 nM,便于临床阈值诊断。
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引用次数: 0
Mango Leaves (Mangifera indica)-Derived Highly Florescent Green Graphene Quantum Dot Nanoprobes for Enhanced On-Off Dual Detection of Cholesterol and Fe2+ Ions Based on Molecular Logic Operation. 基于分子逻辑运算的芒果叶(Mangifera indica)衍生高荧光绿色石墨烯量子点纳米探针用于增强胆固醇和 Fe2+ 离子的开关双重检测。
IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS Pub Date : 2024-07-15 Epub Date: 2024-06-14 DOI: 10.1021/acsabm.4c00292
Ratneshwar Kumar Ratnesh, Mrityunjay Kumar Singh, Vinay Kumar, Snigdha Singh, Ramesh Chandra, Mandeep Singh, Jay Singh

In the present study, we have engineered a molecular logic gate system employing both Fe2+ ions and cholesterol as bioanalytes for innovative detection strategies. We utilized a green-synthesis method employing the mango leaves extract to create fluorescent graphene quantum dots termed "mGQDs". Through techniques like HR-TEM, i.e., high-resolution transmission electron microscopy, Raman spectroscopy, and XPS, i.e., X-ray photoelectron spectroscopy, the successful formation of mGQDs was confirmed. The photoluminescence (PL) characteristics of mGQDs were investigated for potential applications in metal ion detection, specifically Fe2+ traces in water, by using fluorescence techniques. Under 425 nm excitation, mGQDs exhibited emission bands at 495 and 677 nm in their PL spectrum. Fe2+-induced notable quenching of mGQDs' PL intensity decreased by 97% with 2.5 μM Fe2+ ions; however, adding 20 mM cholesterol resulted in a 92% recovery. Detection limits were established through a linear Stern-Volmer (S-V) plot at room temperature, yielding values of 4.07 μM for Fe2+ ions and 1.8 mM for cholesterol. Moreover, mGQDs demonstrated biocompatibility, aqueous solubility, and nontoxicity, facilitating the creation of a rapid nonenzymatic cholesterol detection method. Selectivity and detection studies underscored mGQDs' reliability in cholesterol level monitoring. Additionally, a molecular logic gate system employing Fe2+ metal ions and cholesterol as a bioanalyte was established for detection purposes. Overall, this research introduces an ecofriendly approach to craft mGQDs and highlights their effectiveness in detecting metal ions and cholesterol, suggesting their potential as versatile nanomaterials for diverse analytical and biomedical applications.

在本研究中,我们设计了一种分子逻辑门系统,将 Fe2+ 离子和胆固醇作为生物分析物,用于创新检测策略。我们利用芒果叶提取物的绿色合成方法制造出荧光石墨烯量子点,称为 "mGQDs"。通过 HR-TEM(即高分辨率透射电子显微镜)、拉曼光谱和 XPS(即 X 射线光电子能谱)等技术,证实了 mGQDs 的成功形成。利用荧光技术研究了 mGQDs 的光致发光(PL)特性在金属离子检测(特别是水中的 Fe2+ 痕量)中的潜在应用。在 425 nm 的激发下,mGQDs 的光致发光光谱在 495 和 677 nm 处显示出发射带。在 2.5 μM Fe2+ 离子的作用下,Fe2+ 引起的 mGQDs 聚光强度显著淬灭降低了 97%;然而,加入 20 mM 胆固醇后,其恢复率为 92%。室温下通过线性斯特恩-沃尔默(S-V)图确定了检测限,得出 Fe2+ 离子的检测限为 4.07 μM,胆固醇的检测限为 1.8 mM。此外,mGQDs 还具有生物相容性、水溶性和无毒性,有助于创建一种快速的非酶胆固醇检测方法。选择性和检测研究强调了 mGQDs 在监测胆固醇水平方面的可靠性。此外,还建立了一个采用 Fe2+ 金属离子和胆固醇作为生物分析物的分子逻辑门系统,用于检测目的。总之,这项研究介绍了一种制作 mGQDs 的生态友好型方法,并强调了它们在检测金属离子和胆固醇方面的有效性,表明它们具有作为多功能纳米材料用于各种分析和生物医学应用的潜力。
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引用次数: 0
Ultralow-Power DST-TFET pH Sensor Exceeding the Nernst Limit with Influence of Temperature on Sensitivity. 超低功耗 DST-TFET pH 传感器超越奈恩斯特极限,温度对灵敏度的影响
IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS Pub Date : 2024-07-15 Epub Date: 2024-06-18 DOI: 10.1021/acsabm.4c00428
Nuzhat Yousf, Aadil Anam, Zuber Rasool, S Intekhab Amin

In this paper, a dual-source T-channel TFET (DST-TFET)-based pH sensor demonstrating the pH change in the electrolyte has been studied. The proposed device exceeds the Nernst limit of 59 mV/pH by ∼5 folds. The simulation is performed on an ATLAS TCAD tool (SILVACO) and pH is determined by calculating the interface charge density (Δσ) using appropriate physics models. The voltage sensitivities, using various oxides (SiO2, HfO2, Al2O3) with a maximum achieved sensitivity (SV) of 297.66 mV/pH ∼5× Nernst limit, have been calculated. Moreover, this high value of SV is achieved at an ultralow operating voltage of 0.1 V. The results have been validated by proper calibration of models with the experimental data. It is evident from the results that DST-TFET performs well at ultralow power compared to several previously reported devices. Furthermore, the temperature study has been implemented in the proposed sensor to investigate the IDS-VGS characteristics and sensing performance of the device. The pH sensitivity and voltage sensitivity decreased with increase in temperature. The proposed pH sensor with such high sensitivity is an exclusive choice for pH-sensing applications.

本文研究了一种基于双源 T 沟道 TFET(DST-TFET)的 pH 传感器,它能显示电解液中的 pH 值变化。所提出的器件超过了 59 mV/pH 的 Nernst 限值 5 倍。模拟是在 ATLAS TCAD 工具 (SILVACO) 上进行的,pH 值是通过使用适当的物理模型计算界面电荷密度 (Δσ) 来确定的。使用各种氧化物(SiO2、HfO2、Al2O3)计算出的电压灵敏度最大达到 297.66 mV/pH ∼ 5× Nernst 极限。此外,这一高灵敏度值是在 0.1 V 的超低工作电压下实现的。根据实验数据对模型进行了适当校准,从而验证了计算结果。从结果中可以看出,与之前报道的几种器件相比,DST-TFET 在超低功率下表现良好。此外,还在拟议的传感器中实施了温度研究,以调查该器件的 IDS-VGS 特性和传感性能。pH 灵敏度和电压灵敏度随着温度的升高而降低。所提出的 pH 传感器具有如此高的灵敏度,是 pH 传感应用的不二之选。
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引用次数: 0
Titania-Graphene Oxide Nanocomposite-Based Philadelphia-Positive Leukemia Therapy. 基于钛-石墨烯氧化物纳米复合材料的费城阳性白血病疗法
IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS Pub Date : 2024-07-15 Epub Date: 2024-06-20 DOI: 10.1021/acsabm.4c00207
Maria Batool, Rida-E-Maria Qazi, Muhammad Ahmad Mudassir, Zahra Sajid, Rena Zaman, Mhd Ahmar Rauf, Shazia Kousar, Israr Ahmad, Fawad Ur Rehman, Afsar Ali Mian

Philadelphia-positive (Ph+) leukemia is a type of blood cancer also known as acute lymphoblastic leukemia (ALL), affecting 20-30% of adults diagnosed worldwide and having an engraved prognosis as compared to other types of leukemia. The current treatment regimens mainly rely on tyrosine kinase inhibitors (TKIs) and bone marrow transplants. To date, several generations of TKIs have been developed due to associated resistance and frequent relapse, with cardiovascular system anomalies being the most devastating complication. Nanotechnology has the potential to address these limitations by the targeted drug delivery and controlled release of TKIs. This study focused on the titanium dioxide (TiO2) and graphene oxide (GO) nanocomposite employment to load nilotinib and ponatinib TKIs for therapy of Ph+ leukemia cell line (K562) and Ba/F3 cells engineered to express BCR-ABL oncogene. Meanwhile, after treatment, the oncogene expressing fibroblast cells (Rat-1 P185) were evaluated for their colony formation ability under 3D conditions. To validate the nanocomposite formation, the TiO2-GO nanocomposites were characterized by scanning electron microscope, DLS, XRD, FTIR, zeta potential, EDX, and element mapping. The TKI-loaded TiO2-GO was not inferior to the free drugs after evaluating their effects by a cell viability assay (XTT), apoptosis induction, and colony formation inhibition. The cell signaling pathways of the mammalian target of rapamycin (mTOR), signal transducers and activators of transcription 5 (STAT5), and extracellular signal-regulated kinase (Erk1/2) were also investigated by Western blot. These signaling pathways were significantly downregulated in the TKI-loaded TiO2-GO-treated groups. Based on the findings above, we can conclude that TiO2-GO exhibited excellent drug delivery potential that can be used for Ph+ leukemia therapy in the future, subject to further investigations.

费城阳性(Ph+)白血病是一种血癌,也被称为急性淋巴细胞白血病(ALL),在全球确诊的成人患者中占 20-30%,与其他类型的白血病相比,其预后不容乐观。目前的治疗方案主要依靠酪氨酸激酶抑制剂(TKIs)和骨髓移植。迄今为止,已经开发了几代 TKIs,但都存在耐药性和频繁复发的问题,其中心血管系统异常是最具破坏性的并发症。纳米技术有可能通过 TKIs 的靶向给药和控制释放来解决这些局限性。本研究重点研究了二氧化钛(TiO2)和氧化石墨烯(GO)纳米复合材料负载尼罗替尼和泊纳替尼TKIs,用于治疗Ph+白血病细胞系(K562)和表达BCR-ABL癌基因的Ba/F3细胞。同时,在三维条件下评估了表达癌基因的成纤维细胞(Rat-1 P185)在治疗后的集落形成能力。为了验证纳米复合材料的形成,对 TiO2-GO 纳米复合材料进行了扫描电子显微镜、DLS、XRD、傅立叶变换红外光谱、ZETA 电位、EDX 和元素图谱表征。通过细胞存活率测定(XTT)、细胞凋亡诱导和集落形成抑制等方法评估了TKI负载TiO2-GO的效果,结果显示TiO2-GO并不逊色于游离药物。此外,还通过 Western 印迹研究了雷帕霉素哺乳动物靶标(mTOR)、转录信号转导和激活因子 5(STAT5)以及细胞外信号调节激酶(Erk1/2)的细胞信号通路。这些信号通路在TKI负载的TiO2-GO处理组中明显下调。根据上述研究结果,我们可以得出结论:TiO2-GO 具有出色的药物输送潜力,未来可用于 Ph+ 白血病的治疗,但还有待于进一步研究。
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引用次数: 0
Pro-angiogenic Terbium Hydroxide Nanorods Improve Critical Limb Ischemia in Part by Amelioration of Ischemia-Induced Endothelial Injury. 氢氧化铽纳米棒部分通过改善缺血诱导的内皮损伤来改善重度肢体缺血。
IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS Pub Date : 2024-07-15 Epub Date: 2024-06-07 DOI: 10.1021/acsabm.4c00252
Papia Basuthakur, Arpita Roy, Soumya Ghosh, Vincy Vijay, Debiprasad Sinha, Mydhili Radhakrishnan, Arvind Kumar, Chitta Ranjan Patra, Sumana Chakravarty

Critical limb ischemia (CLI) refers to a severe condition resulting from gradual obstruction in the supply of blood, oxygen, and nutrients to the limbs. The most promising clinical solution to CLI is therapeutic angiogenesis. This study explored the potency of pro-angiogenic terbium hydroxide nanorods (THNR) for treatment of CLI, with a major focus on their impact on ischemia-induced maladaptive alterations in endothelial cells as well as on vascularization in ischemic limbs. This study demonstrated that, in hypoxia-exposed endothelial cells, THNR improve survival and promote proliferation, migration, restoration of nitric oxide production, and regulation of vascular permeability. Based on molecular studies, these attributes of THNR can be traced to the stimulation of PI3K/AKT/eNOS signaling pathways. Besides, Wnt/GSK-3β/β-catenin signaling pathways may also play a role in the therapeutic actions of THNR. Furthermore, in the murine model of CLI, THNR administration can integrally re-establish blood perfusion with concomitant reduction of muscle damage and inflammation. Additionally, improvement of locomotor activities and motor coordination in ischemic limbs in THNR treated mice is also evident. Overall, the study demonstrates that THNR have the potential to be developed as an efficacious and cost-effective alternative clinical therapy for CLI, using a nanomedicine approach.

危重肢体缺血(CLI)是指肢体血液、氧气和营养供应逐渐受阻而导致的严重病症。治疗性血管生成是治疗肢体缺血最有希望的临床解决方案。本研究探讨了氢氧化铽纳米棒(THNR)治疗慢性缺血性心肌梗死的功效,重点关注其对缺血诱导的内皮细胞不良适应性改变以及对缺血肢体血管化的影响。这项研究表明,在缺氧暴露的内皮细胞中,THNR 可提高存活率,促进增殖、迁移、一氧化氮生成的恢复以及血管通透性的调节。根据分子研究,THNR 的这些特性可追溯到对 PI3K/AKT/eNOS 信号通路的刺激。此外,Wnt/GSK-3β/β-catenin 信号通路也可能在 THNR 的治疗作用中发挥作用。此外,在 CLI 小鼠模型中,服用 THNR 可以综合重建血液灌注,同时减少肌肉损伤和炎症。此外,经 THNR 治疗的小鼠缺血肢体的运动活动和运动协调性也有明显改善。总之,该研究表明 THNR 有潜力被开发成一种有效且具有成本效益的替代临床疗法,用于采用纳米医学方法治疗慢性缺血性心肌梗死。
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引用次数: 0
ε-Poly-l-lysine: A Naturally Occurring Biodegradable Polypeptide for Selective Detection of 5-Nitroimidazole Antibiotics in Animal Products and Living Cells via Fluorescence. ε-聚赖氨酸:一种通过荧光选择性检测动物产品和活细胞中 5-硝基咪唑类抗生素的天然生物可降解多肽。
IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS Pub Date : 2024-07-15 Epub Date: 2024-06-12 DOI: 10.1021/acsabm.4c00503
Vishnu N Vijayan, Karthika Kannan, Revathy Sahadevan, Anna Jose, Mintu Porel, Sushabhan Sadhukhan

The 5-nitroimidazole (5-NI) class of antibiotics, such as metronidazole, ornidazole, secnidazole, and tinidazole, are widely used to prevent bacterial infection in humans and livestock industries. However, their overuse contaminates the farmed animal products and water bodies. Hence, a selective, sensitive, and cost-effective method to detect 5-NI antibiotics is the need of the hour. Herein, we report a rapid, inexpensive, and efficient sensing system to detect 5-NI drugs using an as-prepared solution of ε-poly-l-lysine (ε-PL), a naturally occurring and biodegradable homopolypeptide that has an intrinsic fluorescence via clustering-triggered emission. The low nanomolar detection limit (3.25-3.97 nM) for the aforementioned representative 5-NI drugs highlights the sensitivity of the system, outperforming most of the reported sensors alike. The resulting fluorescence quenching was found to be static in nature. Importantly, excellent recovery (100.26-104.41%) was obtained for all real samples and animal products tested. Visual detection was demonstrated by using paper strips and silica gel for practical applications. Furthermore, ε-PL could detect 5-NI antibiotics in living 3T3-L1 mouse fibroblast cells via cellular imaging. Taken together, the present work demonstrates the detection of 5-NI antibiotics using a biocompatible natural polypeptide, ε-PL, and represents a simple and inexpensive analytical tool for practical application.

5-硝基咪唑(5-NI)类抗生素,如甲硝唑、奥硝唑、昔硝唑和替硝唑,被广泛用于预防人类和畜牧业中的细菌感染。然而,它们的过度使用会污染养殖的动物产品和水体。因此,现在需要一种选择性强、灵敏度高、成本效益高的方法来检测 5-NI 抗生素。ε-poly-l-lysine(ε-PL)是一种天然存在的可生物降解的同源多肽,具有通过簇触发发射的本征荧光。对上述代表性 5-NI 药物的检测限低至纳摩尔(3.25-3.97 nM),这凸显了该系统的灵敏度,优于大多数已报道的同类传感器。所产生的荧光淬灭是静态的。重要的是,在测试的所有真实样品和动物产品中都获得了极高的回收率(100.26-104.41%)。在实际应用中,使用纸条和硅胶进行了目视检测。此外,ε-PL 还能通过细胞成像检测活体 3T3-L1 小鼠成纤维细胞中的 5-NI 抗生素。综上所述,本研究利用一种生物相容性天然多肽--ε-PL--证明了对 5-NI 抗生素的检测,并为实际应用提供了一种简单而廉价的分析工具。
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引用次数: 0
Customizable Fabrication of Photothermal Microneedles with Plasmonic Nanoparticles Using Low-Cost Stereolithography Three-Dimensional Printing. 利用低成本立体光刻三维打印技术定制带有等离子纳米粒子的光热微针。
IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS Pub Date : 2024-07-15 Epub Date: 2024-06-15 DOI: 10.1021/acsabm.4c00411
Jill Ziesmer, Isabel Sondén, Justina Venckute Larsson, Padryk Merkl, Georgios A Sotiriou

Photothermal microneedle (MN) arrays have the potential to improve the treatment of various skin conditions such as bacterial skin infections. However, the fabrication of photothermal MN arrays relies on time-consuming and potentially expensive microfabrication and molding techniques, which limits their size and translation to clinical application. Furthermore, the traditional mold-and-casting method is often limited in terms of the size customizability of the photothermal array. To overcome these challenges, we fabricated photothermal MN arrays directly via 3D-printing using plasmonic Ag/SiO2 (2 wt % SiO2) nanoaggregates dispersed in ultraviolet photocurable resin on a commercial low-cost liquid crystal display stereolithography printer. We successfully printed MN arrays in a single print with a translucent, nanoparticle-free support layer and photothermal MNs incorporating plasmonic nanoaggregates in a selective fashion. The photothermal MN arrays showed sufficient mechanical strength and heating efficiency to increase the intradermal temperature to clinically relevant temperatures. Finally, we explored the potential of photothermal MN arrays to improve antibacterial therapy by killing two bacterial species commonly found in skin infections. To the best of our knowledge, this is the first time describing the printing of photothermal MNs in a single step. The process introduced here allows for the translatable fabrication of photothermal MN arrays with customizable dimensions that can be applied to the treatment of various skin conditions such as bacterial infections.

光热微针(MN)阵列具有改善各种皮肤病(如细菌性皮肤感染)治疗的潜力。然而,光热微针阵列的制造依赖于耗时且可能昂贵的微细加工和成型技术,这限制了其尺寸和临床应用。此外,传统的模铸方法往往限制了光热阵列的尺寸定制。为了克服这些挑战,我们在商用低成本液晶显示器立体光刻打印机上,使用分散在紫外线光固化树脂中的等离子体Ag/SiO2(2 wt % SiO2)纳米聚合体,通过3D打印技术直接制造了光热MN阵列。我们成功地在一次打印中打印出了带有半透明、无纳米颗粒支撑层的 MN 阵列,并以选择性方式打印出了含有等离子纳米沉积物的光热 MN。光热 MN 阵列显示出足够的机械强度和加热效率,可将皮内温度提高到临床相关温度。最后,我们探索了光热 MN 阵列通过杀死皮肤感染中常见的两种细菌来改善抗菌治疗的潜力。据我们所知,这是首次描述光热 MN 的单步打印。本文介绍的工艺可以转化制造出尺寸可定制的光热 MN 阵列,可用于治疗细菌感染等各种皮肤病。
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引用次数: 0
3D-Printed Demineralized Bone Matrix-Based Conductive Scaffolds Combined with Electrical Stimulation for Bone Tissue Engineering Applications. 基于脱矿物质骨基质的三维打印导电支架与骨组织工程应用中的电刺激相结合。
IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS Pub Date : 2024-07-15 Epub Date: 2024-06-21 DOI: 10.1021/acsabm.4c00236
Damion T Dixon, Erika N Landree, Cheryl T Gomillion

Bone is remodeled through a dynamic process facilitated by biophysical cues that support cellular signaling. In healthy bone, signaling pathways are regulated by cells and the extracellular matrix and transmitted via electrical synapses. To this end, combining electrical stimulation (ES) with conductive scaffolding is a promising approach for repairing damaged bone tissue. Therefore, "smart" biomaterials that can provide multifunctionality and facilitate the transfer of electrical cues directly to cells have become increasingly more studied in bone tissue engineering. Herein, 3D-printed electrically conductive composite scaffolds consisting of demineralized bone matrix (DBM) and polycaprolactone (PCL), in combination with ES, for bone regeneration were evaluated for the first time. The conductive composite scaffolds were fabricated and characterized by evaluating mechanical, surface, and electrical properties. The DBM/PCL composites exhibited a higher compressive modulus (107.2 MPa) than that of pristine PCL (62.02 MPa), as well as improved surface properties (i.e., roughness). Scaffold electrical properties were also tuned, with sheet resistance values as low as 4.77 × 105 Ω/sq for our experimental coating of the highest dilution (i.e., 20%). Furthermore, the biocompatibility and osteogenic potential of the conductive composite scaffolds were tested using human mesenchymal stromal cells (hMSCs) both with and without exogenous ES (100 mV/mm for 5 min/day four times/week). In conjunction with ES, the osteogenic differentiation of hMSCs grown on conductive DBM/PCL composite scaffolds was significantly enhanced when compared to those cultured on PCL-only and nonconductive DBM/PCL control scaffolds, as determined through xylenol orange mineral staining and osteogenic protein analysis. Overall, these promising results suggest the potential of this approach for the development of biomimetic hybrid scaffolds for bone tissue engineering applications.

骨骼是通过支持细胞信号传导的生物物理线索促进的动态过程进行重塑的。在健康的骨骼中,信号通路由细胞和细胞外基质调节,并通过电突触传递。为此,将电刺激(ES)与导电支架相结合是修复受损骨组织的一种很有前景的方法。因此,在骨组织工程中,越来越多的人开始研究能提供多功能性并促进电线索直接传递到细胞的 "智能 "生物材料。本文首次评估了由脱矿骨基质(DBM)和聚己内酯(PCL)组成的三维打印导电复合支架与 ES 的结合在骨再生中的应用。通过评估力学、表面和电学特性,制作并鉴定了导电复合支架。与原始 PCL(62.02 兆帕)相比,DBM/PCL 复合材料表现出更高的压缩模量(107.2 兆帕)以及更好的表面特性(即粗糙度)。脚手架的电气性能也得到了调整,在最高稀释度(即 20%)的实验涂层中,片层电阻值低至 4.77 × 105 Ω/sq。此外,我们还使用人间质基质细胞(hMSCs)测试了导电复合材料支架的生物相容性和成骨潜能,包括外源性 ES(100 mV/mm,5 分钟/天,4 次/周)和不外源性 ES(100 mV/mm,5 分钟/天,4 次/周)。通过二甲酚橙矿物质染色和成骨蛋白分析确定,与在纯 PCL 和非导电 DBM/PCL 对照支架上培养的 hMSCs 相比,在添加 ES 的情况下,生长在导电 DBM/PCL 复合支架上的 hMSCs 的成骨分化能力明显增强。总之,这些令人鼓舞的结果表明,这种方法具有开发骨组织工程应用的仿生混合支架的潜力。
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引用次数: 0
Nanofibrous Microspheres: A Biomimetic Platform for Bone Tissue Regeneration. 纳米纤维微球:骨组织再生的仿生平台。
IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS Pub Date : 2024-07-15 Epub Date: 2024-07-01 DOI: 10.1021/acsabm.4c00613
Nimeet Desai, Shreya Pande, Lalitkumar K Vora, Nagavendra Kommineni

Bone, a fundamental constituent of the human body, is a vital scaffold for support, protection, and locomotion, underscoring its pivotal role in maintaining skeletal integrity and overall functionality. However, factors such as trauma, disease, or aging can compromise bone structure, necessitating effective strategies for regeneration. Traditional approaches often lack biomimetic environments conducive to efficient tissue repair. Nanofibrous microspheres (NFMS) present a promising biomimetic platform for bone regeneration by mimicking the native extracellular matrix architecture. Through optimized fabrication techniques and the incorporation of active biomolecular components, NFMS can precisely replicate the nanostructure and biochemical cues essential for osteogenesis promotion. Furthermore, NFMS exhibit versatile properties, including tunable morphology, mechanical strength, and controlled release kinetics, augmenting their suitability for tailored bone tissue engineering applications. NFMS enhance cell recruitment, attachment, and proliferation, while promoting osteogenic differentiation and mineralization, thereby accelerating bone healing. This review highlights the pivotal role of NFMS in bone tissue engineering, elucidating their design principles and key attributes. By examining recent preclinical applications, we assess their current clinical status and discuss critical considerations for potential clinical translation. This review offers crucial insights for researchers at the intersection of biomaterials and tissue engineering, highlighting developments in this expanding field.

骨骼是人体的基本组成成分,是支撑、保护和运动的重要支架,在维持骨骼完整性和整体功能方面发挥着关键作用。然而,创伤、疾病或衰老等因素都会损害骨骼结构,因此必须采取有效的再生策略。传统方法往往缺乏有利于高效组织修复的仿生环境。纳米纤维微球(NFMS)通过模拟原生细胞外基质结构,为骨再生提供了一个前景广阔的仿生平台。通过优化制造技术和加入活性生物分子成分,NFMS 可以精确复制促进骨生成所必需的纳米结构和生化线索。此外,NFMS 还具有多种特性,包括可调形态、机械强度和可控释放动力学,使其更适用于定制骨组织工程应用。NFMS 可增强细胞募集、附着和增殖,同时促进成骨分化和矿化,从而加速骨愈合。本综述强调了 NFMS 在骨组织工程中的关键作用,阐明了它们的设计原理和关键属性。通过研究最近的临床前应用,我们评估了它们目前的临床状态,并讨论了潜在临床转化的关键考虑因素。这篇综述为生物材料和组织工程交叉领域的研究人员提供了重要的见解,突出了这一不断扩大的领域的发展。
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引用次数: 0
Graphene Multiplexed Sensor for Point-of-Need Viral Wastewater-Based Epidemiology. 基于病毒性废水流行病学的石墨烯复用传感器
IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS Pub Date : 2024-07-15 Epub Date: 2024-07-02 DOI: 10.1021/acsabm.4c00484
Michael Geiwitz, Owen Rivers Page, Tio Marello, Marina E Nichols, Narendra Kumar, Stephen Hummel, Vsevolod Belosevich, Qiong Ma, Tim van Opijnen, Bruce Batten, Michelle M Meyer, Kenneth S Burch

Wastewater-based epidemiology (WBE) can help mitigate the spread of respiratory infections through the early detection of viruses, pathogens, and other biomarkers in human waste. The need for sample collection, shipping, and testing facilities drives up the cost of WBE and hinders its use for rapid detection and isolation in environments with small populations and in low-resource settings. Given the ubiquitousness and regular outbreaks of respiratory syncytial virus, SARS-CoV-2, and various influenza strains, there is a rising need for a low-cost and easy-to-use biosensing platform to detect these viruses locally before outbreaks can occur and monitor their progression. To this end, we have developed an easy-to-use, cost-effective, multiplexed platform able to detect viral loads in wastewater with several orders of magnitude lower limit of detection than that of mass spectrometry. This is enabled by wafer-scale production and aptamers preattached with linker molecules, producing 44 chips at once. Each chip can simultaneously detect four target analytes using 20 transistors segregated into four sets of five for each analyte to allow for immediate statistical analysis. We show our platform's ability to rapidly detect three virus proteins (SARS-CoV-2, RSV, and Influenza A) and a population normalization molecule (caffeine) in wastewater. Going forward, turning these devices into hand-held systems would enable wastewater epidemiology in low-resource settings and be instrumental for rapid, local outbreak prevention.

基于废水的流行病学(WBE)可以通过早期检测人类排泄物中的病毒、病原体和其他生物标记物来帮助减少呼吸道感染的传播。样本收集、运输和检测设施的需求增加了污水流行病学的成本,阻碍了其在人口稀少和资源匮乏环境中的快速检测和隔离。鉴于呼吸道合胞病毒、SARS-CoV-2 和各种流感病毒无处不在且定期爆发,人们越来越需要一种低成本、易于使用的生物传感平台,以便在病毒爆发前进行本地检测,并监测其进展情况。为此,我们开发了一种易于使用、经济高效的多重平台,能够检测废水中的病毒载量,其检测限比质谱法低几个数量级。这得益于晶圆级生产和与连接分子预连接的适配体,一次可生产 44 块芯片。每个芯片可同时检测四个目标分析物,使用 20 个晶体管,每个分析物分为四组,每组五个,以便立即进行统计分析。我们展示了我们的平台快速检测废水中三种病毒蛋白(SARS-CoV-2、RSV 和甲型流感)和一种群体归一化分子(咖啡因)的能力。展望未来,将这些设备转化为手持式系统将有助于在资源匮乏的环境中开展废水流行病学研究,并有助于快速预防当地疫情爆发。
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