Food & Function最新文献

Increasing evidence suggests that carotenoids play an important role in visual and cognitive development during early life. This study aimed to depict the carotenoid profile in maternal/cord plasma and breast milk in three northern cities of China while investigating the association between dietary carotenoid intake and breast milk carotenoid levels. We enrolled 990 lactating mothers from three urban northern Chinese cities to collect breast milk (including colostrum, transitional milk, early mature milk, middle mature milk, and late mature milk). Among them, 90 participants also provided maternal/cord blood. The dietary carotenoid intake was recorded in the corresponding stages according to the Food Frequency Questionnaire and 24-hour dietary recall. The levels of carotenoids (lutein, zeaxanthin, β-cryptoxanthin, β-carotene, and lycopene) in maternal/cord plasma and breast milk were analyzed using high-performance liquid chromatography. We found a significant correlation between carotenoid concentrations in maternal and cord plasma. Moreover, carotenoid concentrations in maternal plasma were significantly higher than those in cord plasma. The total carotenoid levels in breast milk decreased from colostrum to late mature milk. β-Carotene predominated in colostrum, whereas lutein dominated in transitional and mature milk. We also found that breast milk carotenoid levels correlated with dietary carotenoid intake to a certain extent, particularly during the early stages of lactation. Therefore, we encourage women to appropriately increase their dietary intake of carotenoid-rich vegetables and fruits during pregnancy and lactation, particularly during work hours after lactation leave. Moreover, the variance of the carotenoid profile in maternal/cord plasma and breast milk across the three cities implied the necessity of further depicting the carotenoid status and influencing factors across different areas in China.

Low molecular weight galactomannan (LMGM), a soluble dietary fibre derived from guar gum, is recognized for its prebiotic functions, including promoting the growth of beneficial intestinal bacteria and the production of short-chain fatty acids, but the mechanism of alleviating diarrhea is not fully understood. This study established an acute diarrhea mouse model using senna leaf decoction and evaluated the therapeutic effects of LMGM by monitoring diarrhea scores, loose stool prevalence, intestinal tissue pathology and gene expression, and gut microbiota composition and metabolisms. The results indicated that LMGM significantly reduced diarrhea scores and loose stool prevalence within two hours post-treatment. Hematoxylin and eosin staining and quantitative real-time polymerase chain reaction analysis revealed that LMGM improved intestinal epithelial structure and up-regulated the expression of zonula occludens 1, occludin, mucin 2, aquaporin 3, and aquaporin 4 in ileum, jejunum, and colon tissues. Moreover, LMGM increased the abundance of beneficial bacteria such as Lactobacillaceae and Lachnospiraceae, and decreased Prevotellaceae in the cecum. Furthermore, LMGM promoted short-chain fatty acid production and reduced ammonia nitrogen and skatole concentrations in the intestinal content. The study suggests that LMGM could serve as a functional prebiotic for diarrhea alleviation, potentially by enhancing the intestinal barrier, modulating water transportation, and regulating the microbiota composition.

Over the last decade, research has emphasized the role of the microbiome in regulating cardiovascular physiology and disease progression. Understanding the interplay between wine polyphenols, the gut microbiota, and cardiovascular health could provide valuable insights for uncovering novel therapeutic strategies aimed at preventing and managing cardiovascular disease. In this study, two commercial red wines were subjected to in vitro dynamic gastrointestinal digestion (GIS) to monitor the flavanol-microbiota interaction by evaluating the resulting microbial metabolites. Furthermore, the cardiovascular protective activity of wine flavanol microbial metabolites was investigated, integrating their effects on antihypertensive activity, cholesterol metabolism and insulin resistance into human endothelial (EA.hy926) and hepatic (HepG2) cell lines. A significant production of microbial flavanol metabolites, with a prevalence of phenylpropionic and phenylacetic acids, valerolactones and short chain fatty acids such as butyric acid, was observed, particularly in the transverse and descending colon sections. Incubating HAECs and HepG2 cells with the colon improved cardioprotective parameters. Specifically, an increase in the vasodilator NO, an improvement in the LDL receptors and the HMGCoA enzyme, with positive effects on cholesterol metabolism, and the reduction of glycogen levels improving insulin resistance were observed.

Bacillus amyloliquefaciens, a potential probiotic for use in food and feed production, can exert anti-aging effects in a strain-specific manner. However, the molecular mechanisms underlying its anti-aging effects remain poorly understood. This study explored the effects of B. amyloliquefaciens WF2020 (WF2020), isolated from Chinese fermented pickles, on longevity and health and investigated the underlying mechanisms in Caenorhabditis elegans. Interestingly, WF2020 was found to increase mean lifespan, worm length, body bends, and resistance to heat, oxidative stress, and Staphylococcus aureus infection. It also improved mitochondrial transmembrane potential and antioxidative status, reduced lipid accumulation and oxidative damage, and altered the expression of several genes involved in cell apoptosis, fat metabolism, host tolerance to heat and oxidative stress, and immune responses, compared to Escherichia coli OP50 (OP50), a standard food source for C. elegans. Moreover, WF2020-fed loss-of-function mutants for nsy-1, sek-1, pmk-1, jkk-1, jnk-1, daf-16, and hsf-1 did not exhibit lifespan extension. Additionally, WF2020-fed pmk-1 and jnk-1 mutants showed similar worm length, body bends, lipid accumulation, mitochondrial transmembrane potential, and antioxidative properties to the OP50 group. Correspondingly, WF2020 significantly upregulated the expression of nsy-1, sek-1, pmk-1, jkk-1, and jnk-1 and increased the proportion of DAF-16::GFP in the nucleus, along with the expression of HSP-16.2::GFP. In conclusion, WF2020 activated the p38 MAPK and JNK pathways to regulate the functions of HSF-1 and DAF-16, thereby promoting longevity and health in C. elegans. These findings suggest that WF2020 could be a potential probiotic or a starter for use in food and feed production to delay aging and promote health.

Lactopontin (LPN) is an important milk protein with the potential to improve bone health; however, its specific effects have not been determined. This study aims to investigate the effects of LPN on early bone growth and development. 3 week-old SD rats (n = 32) were assigned to the control group, whey protein concentration (WPC) group, LPN-L (low-dose LPN) group, and LPN-H (high-dose LPN) group, with intragastric administration of deionized water, 65.8 mg kg^-1 WPC, and 5 and 45 mg kg^-1 LPN for 30 days, respectively. LPN-H supplementation increased body length and femur length, improved femoral bone volume and microarchitecture, and upregulated osteoblast activity-related mRNA (Bmp2, Smad8, and Runx-2) expression in the femur. The content of secondary bile acid glycolithocholic acid (GLCA) in stool and serum was significantly increased after LPN-H intervention and positively correlated with the increased abundance of Parabacteroides in feces. In addition, the bile acid receptor FXR in femur was also activated in the LPN-H group, suggesting that LPN may regulate bone health through the microbiota-metabolite-bone axis. The results of this study suggest that LPN has potential application value in regulating bone metabolism during growth.

This study is the first to explore the effects of the novel yellow pigment monascinol (Msol) from red mold rice (RMR) on reducing body fat and to compare its effects with those of monascin (MS) and ankaflavin (AK). In a high-fat diet-induced rat model, different doses of RMR fermented rice (RL, RM, RH) and purified Msol, MS, and AK were administered over an 8-week period. The results showed that all treatment groups significantly reduced body weight and fat mass. Msol, in particular, activated acetyl-CoA carboxylase (ACC), inhibiting fatty acid synthesis and reducing triglyceride accumulation. All treatments suppressed the differentiation of preadipocytes into mature adipocytes by inhibiting CCAAT/enhancer-binding proteins β (C/EBPβ) and C/EBPα, as well as peroxisome proliferator-activated receptor γ (PPARγ). In the liver, RL, RM, RH, MS, and AK enhanced the expression of AMP-activated protein kinase (AMPK), ACC, peroxisome proliferator-activated receptor α (PPARα), and carnitine palmitoyl transferase-1α (CPT-1α), thereby promoting fatty acid metabolism. Additionally, RMR and its active components, MS and Msol, reduced body fat by modulating gut microbiota. These compounds significantly decreased the abundance of bacteria associated with fat storage, such as Oliverpabstia intestinalis, while increasing the abundance of bacteria linked to energy expenditure and lipid breakdown, such as Akkermansia muciniphila and Ruminococcus callidus. Moreover, MS and Msol upregulated proteins involved in fat degradation, such as UCP1, thereby enhancing fat burning and reducing fat accumulation. These regulatory effects suggest that Monascus and its components have potential in managing metabolic health and reducing obesity.

Rheumatoid arthritis (RA) is a systemic, chronic autoimmune disease. Many studies have shown that microorganisms may be an important pathological factor leading to the onset of RA. Some infectious or non-infectious pathogenic microorganisms and their metabolites may be the initiating factors of the early onset of RA. The aim of this study was to alleviate RA by regulating the imbalance of the gut microbiota in the early stage of RA using mixed bacterial strains. The mixed strains of Lactobacillus acidophilus (BD18, NCFM, BD1802, BD3545, BD5032) significantly reduced the clinical score and swelling thickness of the paws of collagen-induced arthritis (CIA) rats. The expression of TNF-α and MMP-13 proteins in the joints of CIA rats was also inhibited. The levels of specific antibodies (anti-CII IgG, anti-CII IgG1, anti-CII IgG2a, and anti-CII IgG2b) and inflammatory factor IL-6 in the serum of CIA rats were also significantly reduced. The relative abundance of Lactobacillus, Clostridia_UCG-014, Ruminococcus, Candidatus_Saccharimonas, Romboutsia, Turicibacter, and Clostridium_sensu_stricto_1 in the gut microbiota of CIA rats receiving oral administration of the mixed strains of L. acidophilus was significantly reduced, bringing their microbiota closer to that of healthy rats. Moreover, the levels of intestinal metabolites of short chain fatty acids in CIA rats, acetic acid and butyric acid, were significantly increased. The mixed strains of L. acidophilus could recover the relative abundance of Ligilactobacillus, Clostridia_UCG-014_unclassified, Ruminococcus, Candidatus_Saccharimonas, Romboutsia and Turicibacter, affecting purine metabolism, transcription factors and alanine metabolism, and reducing the levels of specific antibodies and inflammatory factors in the blood, finally slowing the development of experimental arthritis induced by CII, which exerts the protective effect of probiotics. Mixed L. acidophilus strains are more effective than a single strain, and the effect is not the result of a single strain, but of a combination of strains.

Pectin is an acidic heteropolysaccharide with natural, green, and inexpensive characteristics. Compared to polysaccharides, oligosaccharides are more easily utilized by the body, and the physiological function of hawthorn pectin oligosaccharides (POS) may vary depending on their degree of polymerization (DP). Therefore, we mainly studied the effects of hawthorn pectin (HP) and POS with different DP on gut microbiota disorders induced by high-fat diet (HFD). HP and POS both improved weight gain, dyslipidemia, and glucose homeostasis caused by HFD, and increased serum GLP-1 levels. Meanwhile, the increased expression of Gcg and Pcsk1 genes in the ileum of the treatment group further confirmed this result. In addition, HP and POS reduced certain opportunistic pathogens, while restoring the richness and diversity of the gut microbiota. Meanwhile, HP and POS can improve intestinal barrier dysfunction by increasing the claudin-1, occludin, ZO-1, and MUC2 genes. Furthermore, fecal metabolomics suggests that POS may enhance linoleic acid synthesis and improve lipid metabolism by upregulating 9,10-DHOME ((12Z)-9,10-dihydroxyoctadec-12-enoic acid), while HP cannot. Overall, the research results indicate that both HP and POS can improve the weight phenotype changes, gut microbiota disruption, and metabolites changes caused by HFD. Particularly, POS has a better effect than HP, and there are differences in the improvement effect of POS with different DP, among which POS with DP 5 has the most significant improvement effect. This discovery enhances a deeper comprehension of the biological activity of different POS, providing an important basis for further optimizing the application of POS as a functional food.

Impairment of gut barrier integrity is associated with the pathogenesis of gastrointestinal diseases, including inflammatory bowel disease, colorectal cancer, and coeliac disease. While many aspects of diet have been linked to improved barrier function, (poly)phenols, a broad group of bioactive phytochemicals, are of potential interest. The (poly)phenolic sub-class, flavan-3-ols, have been investigated in some detail owing to their abundance in commonly consumed foods, including grapes, tea, apples, cocoa, berries, and nuts. This review summarises studies on the effects of flavan-3-ols, their microbiome-mediated metabolites, and food sources of these compounds, on gut barrier structure. Extensive evidence demonstrates that flavan-3-ol rich foods, individual flavan-3-ols (e.g., (epi)catechin, epi(gallo)catechin-3-O-gallate, and pro(antho)cyanidins), and their related microbiota-mediated metabolites, could be effective in protecting and restoring the integrity of the gut barrier. In this context, various endpoints are assessed, including transepithelial electrical resistance of the epithelial layer and expression of tight junction proteins and mucins, in ex vivo, in vitro, and animal models. The differences in bioactivity reported for barrier integrity are structure-function dependent, related to the (poly)phenolic source or the tested compound, as well as their dose, exposure time, and presence or absence of a stressor in the experimental system. Overall, these results suggest that flavan-3-ols and related compounds could help to maintain, protect, and restore gut barrier integrity, indicating that they might contribute to the beneficial properties associated with the intake of their dietary sources. However, rigorous and robustly designed human intervention studies are needed to confirm these experimental observations.

Background: carbohydrate-restricted diets (CRDs) have gained attention to address metabolic dysregulation commonly observed in dyslipidemia, a condition posing significant risks to cardiovascular health. However, the effectiveness of CRDs in improving cardiovascular health remains contentious. This meta-analysis comprehensively evaluated the long-term effects of CRDs on glucolipid metabolism and weight loss in individuals with dyslipidemia. Methods: extensive searches were conducted in PubMed, Web of Science, Scopus, the Cochrane Library, and EMBASE. Randomized controlled trials examining the effects of CRDs on glucolipid metabolism and weight loss in adults with dyslipidemia over a minimum of three weeks were included. This analysis compared the differential effects between moderate-low carbohydrate diets (MLCDs) and low carbohydrate diets (LCDs), including a targeted evaluation of animal-based CRDs and dyslipidemic individuals based on the BMI status, thereby addressing gaps in current knowledge. Results: Our findings indicated that CRDs significantly enhanced lipid profiles, including total cholesterol (TC), triglycerides (TG), high-density lipoprotein cholesterol (HDL-C), and apolipoprotein B (ApoB), and contributed to weight management in individuals with dyslipidemia. MLCDs were more effective than LCDs in improving all lipid profiles except for TG, which was more effectively managed by LCDs. Animal-based CRDs did not significantly impact lipid profiles. Dyslipidemic individuals with overweight and obesity showed significant changes in TG and ApoB. A noteworthy negative correlation was also observed between TC, TG, and low-density lipoprotein cholesterol levels with higher dietary fiber intake, supporting the beneficial impact of fiber on cardiovascular health. Conclusions: These results for the first time highlighted the potential of adopting MLCDs, particularly those with sufficient fiber content, as a powerful strategy for reducing the risk of cardiovascular diseases in patients suffering from dyslipidemia.