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Outstanding Reviewers for Food & Function in 2023. 2023 年《食品与功能》杰出评论员。
IF 5.1 1区 农林科学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-07-15 DOI: 10.1039/d4fo90060j

We would like to take this opportunity to thank all of Food & Function reviewers for helping to preserve quality and integrity in the chemical science literature. We would also like to highlight our Outstanding Reviewers for Food & Function in 2023.

我们想借此机会感谢《食品与功能》的所有审稿人,感谢他们帮助维护化学科学文献的质量和完整性。此外,我们还想重点介绍 2023 年《食品与功能》的杰出审稿人。
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引用次数: 0
Biomarkers of food intake and their relevance to metabolic syndrome. 食物摄入的生物标志物及其与代谢综合征的关系。
IF 5.1 1区 农林科学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-07-15 DOI: 10.1039/d4fo00721b
Miguel Cifuentes, Farhad Vahid, Yvan Devaux, Torsten Bohn

Metabolic syndrome (MetS) constitutes a prevalent risk factor associated with non communicable diseases such as cardiovascular disease and type 2 diabetes. A major factor impacting the etiology of MetS is diet. Dietary patterns and several individual food constituents have been related to the risk of developing MetS or have been proposed as adjuvant treatment. However, traditional methods of dietary assessment such as 24 h recalls rely greatly on intensive user-interaction and are subject to bias. Hence, more objective methods are required for unbiased dietary assessment and efficient prevention. While it is accepted that some dietary-derived constituents in blood plasma are indicators for certain dietary patterns, these may be too unstable (such as vitamin C as a marker for fruits/vegetables) or too broad (e.g. polyphenols for plant-based diets) or reflect too short-term intake only to allow for strong associations with prolonged intake of individual food groups. In the present manuscript, commonly employed biomarkers of intake including those related to specific food items (e.g. genistein for soybean or astaxanthin and EPA for fish intake) and novel emerging ones (e.g. stable isotopes for meat intake or microRNA for plant foods) are emphasized and their suitability as biomarker for food intake discussed. Promising alternatives to plasma measures (e.g. ethyl glucuronide in hair for ethanol intake) are also emphasized. As many biomarkers (i.e. secondary plant metabolites) are not limited to dietary assessment but are also capable of regulating e.g. anti-inflammatory and antioxidant pathways, special attention will be given to biomarkers presenting a double function to assess both dietary patterns and MetS risk.

代谢综合征(MetS)是与心血管疾病和 2 型糖尿病等非传染性疾病相关的普遍风险因素。影响代谢综合征病因的一个主要因素是饮食。膳食模式和几种食物成分与 MetS 的发病风险有关,或被建议作为辅助治疗手段。然而,传统的膳食评估方法(如 24 小时回忆)在很大程度上依赖于大量的用户互动,而且容易产生偏差。因此,需要更客观的方法来进行无偏见的膳食评估和有效的预防。虽然血浆中的某些膳食成分被认为是某些膳食模式的指标,但这些指标可能过于不稳定(如作为水果/蔬菜标志物的维生素 C)或过于宽泛(如植物性膳食中的多酚),或仅反映短期摄入量,无法与个别食物组的长期摄入量建立密切联系。本手稿强调了常用的摄入量生物标志物,包括与特定食物有关的生物标志物(如大豆中的染料木素或鱼类摄入量中的虾青素和 EPA)和新出现的生物标志物(如肉类摄入量中的稳定同位素或植物性食物中的微 RNA),并讨论了它们作为食物摄入量生物标志物的适用性。此外,还强调了有望替代血浆测量方法的其他方法(例如,用头发中的葡萄糖醛酸乙酯来衡量乙醇摄入量)。由于许多生物标志物(如次生植物代谢物)不仅限于膳食评估,还能调节抗炎和抗氧化途径等,因此将特别关注具有双重功能的生物标志物,以评估膳食模式和 MetS 风险。
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引用次数: 0
Greater adherence to the Mediterranean Dietary Approaches to Stop Hypertension Intervention for Neurodegenerative Delay (MIND) diet is associated with lower risk of inflammatory bowel disease: a prospective cohort study. 更多坚持地中海饮食法治疗高血压干预神经退行性延迟(MIND)与降低炎症性肠病风险相关:一项前瞻性队列研究。
IF 5.1 1区 农林科学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-07-15 DOI: 10.1039/d4fo00641k
Shuyu Ye, Yuhao Sun, Xuejie Chen, Tian Fu, Xixian Ruan, Lintao Dan, Jie Chen, Zhongyan Du, Xiaoyan Wang

Background: The Mediterranean-DASH Intervention for Neurodegenerative Delay (MIND) diet is emerging as a promising candidate for preventive measures against inflammatory bowel disease (IBD), though there is currently no direct evidence from population-based studies. This study aims to bridge the gap in understanding of the association of the MIND diet with IBD risk. Methods: We utilized data from 187 490 participants in the UK Biobank who provided dietary information and were free of IBD at baseline. Dietary information was obtained using a validated web-based 24-hour dietary recall questionnaire. A MIND diet score was evaluated based on the intake of ten beneficial and five unhealthy food groups and the scores were further grouped into tertiles. The outcome of interest was incident IBD, Crohn's disease (CD), and ulcerative colitis (UC). Multivariable-adjusted hazard ratios (HRs) and 95% confidence intervals (CIs) were estimated using Cox proportional hazard models adjusted for demographic characteristics, lifestyle factors, cancer history, and other dietary factors. Mediation analyses were performed to evaluate the role of systemic inflammation and metabolic disorders represented by the integrated biomarkers in the MIND diet-IBD association. Results: After a mean follow-up of 10.7 years, we documented 825 incident IBD cases (250 CD and 575 UC). The average age of the participants was 56.2 years, of which 55.0% were females. We found that greater adherence to the MIND diet, represented by a higher diet score, was associated with a lower risk of IBD (HRcomparing extreme tertiles 0.74, 95% CI 0.62-0.90, p = 0.002; p for trend = 0.005), CD (HR 0.66, 95% CI 0.47-0.94, p = 0.022; p for trend = 0.023), and UC (HR 0.78, 95% CI 0.62-0.98, p = 0.031; p for trend = 0.022). The associations were partially mediated by metabolic and inflammation status (mediation proportion: 5.5-15.9%). Conclusion: We found higher adherence to the MIND diet was associated with a lower risk of IBD, and that inflammatory and metabolic conditions may play an important role in the underlying mechanistic pathways.

背景:地中海-DASH 神经退行性延迟干预疗法(MIND)饮食正在成为预防炎症性肠病(IBD)的一种有希望的候选饮食,尽管目前还没有来自人群研究的直接证据。本研究旨在弥补人们对 MIND 饮食与 IBD 风险之间关系的认识差距。研究方法我们利用了英国生物库中 187 490 名参与者的数据,这些参与者提供了饮食信息,并且在基线时没有 IBD。饮食信息通过经过验证的基于网络的 24 小时饮食回忆问卷获得。根据十种有益食物和五种不健康食物的摄入量评估了 MIND 饮食得分,并将得分进一步分为三等分。研究的结果是IBD、克罗恩病(CD)和溃疡性结肠炎(UC)的发病率。使用Cox比例危险模型估算了经多变量调整的危险比(HRs)和95%置信区间(CIs),并对人口统计学特征、生活方式因素、癌症病史和其他饮食因素进行了调整。还进行了中介分析,以评估综合生物标志物所代表的全身炎症和代谢紊乱在 MIND 饮食-鼻咽癌关联中的作用。研究结果经过平均 10.7 年的随访,我们记录了 825 例 IBD 病例(250 例 CD 和 575 例 UC)。参与者的平均年龄为 56.2 岁,其中 55.0% 为女性。我们发现,对 MIND 饮食的坚持程度越高(即饮食得分越高),患 IBD 的风险就越低(与极端三等分比较的 HR 值为 0.74,95% CI 为 0.62-0.90,p = 0.002;趋势 p = 0.005)、CD(HR 0.66,95% CI 0.47-0.94,p = 0.022;趋势 p = 0.023)和 UC(HR 0.78,95% CI 0.62-0.98,p = 0.031;趋势 p = 0.022)。新陈代谢和炎症状况对上述关联有部分调节作用(调节比例:5.5%-15.9%)。结论我们发现,较高的 MIND 饮食坚持率与较低的 IBD 风险相关,而炎症和代谢状况可能在潜在的机理途径中发挥重要作用。
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引用次数: 0
Hop (Humulus lupulus L.) extract reverts glycaemic imbalance and cognitive impairment in an animal model of obesity. 蛇麻草(Humulus lupulus L.)提取物可恢复肥胖动物模型的血糖失衡和认知障碍。
IF 5.1 1区 农林科学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-07-15 DOI: 10.1039/d4fo02062f
Mariana da Rocha Alves, Roberto de Paula Nascimento, Ana Paula da Fonseca Machado, Philipe Dos Santos, Eduardo Aledo, Milena Morandi Vuolo, Carolina Oliveira Cavalheiro, Vinícius Oliveira Giaculi, Patrícia Berilli, Nathália Medina Dos Santos, Mario Roberto Marostica Junior

The rates of overweight and obesity around the world have increased in past years. The body's adipose tissue stimulates the antioxidant and oxidation imbalance capacity at the cellular level. This scenario favors an inflammatory low-grade systemic condition starting with insulin resistance, which in turn may involve diabetes mellitus type 2 and cognitive decline afterward. Neurological diseases have been correlated to senile age diseases over time. This scenario calls for a change in the incidence of obesity in the younger generation. An unhealthy dietary consumption together with sedentary habits might lead to poor gut absorption of nutrients. Several plants and foods have bioactive compounds that can reduce or inhibit radical scavengers, reactive oxygen species, and metal ion complexes that threaten the cerebral defense system. The bitter acids from hops (Humulus lupulus L.) have been demonstrated to have promising effects on lipid and carbohydrate metabolism improvement, reducing inflammatory responses through alpha acids, beta acids, and analogs action. Therefore, the current study aimed to investigate the bioactivity of hop bitter acids in obese and lean mice. For that, a dry hop extract (DHE) was obtained by applying carbon dioxide as the fluid of supercritical extraction. Afterward, seventy-eight male mice of the C57BL/6J strain were weighed and randomly distributed into six groups of 13 animals each according to the diet offered: (NO) normolipidic diet, (NO1) normolipidic diet containing 0.35% alpha acids, (NO2) normolipidic diet containing 3.5% alpha acids, (HP) hyperlipidic diet, (HP1) hyperlipidic diet containing 0.35% alpha acids, and (HP2) hyperlipidic diet containing 3.5% alpha acids. After applying the glycemic tolerance and insulin tolerance tests, a better stabilization of glycemia levels and weight gain among those animals fed with DHE (NO2 and HP2) were observed in comparison to the obese control group (HP) (p < 0.05). There was also an amelioration of antioxidant capacity observed by checking the enzymatic profile by SOD and an apparent mitigation of brain degeneration by checking GSK3β and p-IRS1 proteins expression (p < 0.05). The y-maze cognitive test applied to highlight possible obesity-harmful animal brains did not indicate a statistical difference between the groups. Although the weekly dietary intake between the obese HP2 group (33.32 ± 4.11, p < 0.05) and control HP (42.3 ± 5.88, p < 0.05) was different. The bioactive compounds present in DHE have demonstrated relevant effects on glycemic control, insulin signaling, and the consequent modulatory action of the obesity-related markers with the brain's inflammatory progression.

近年来,全球超重和肥胖的比例不断上升。人体的脂肪组织会在细胞水平上刺激抗氧化和氧化失衡能力。这种情况有利于从胰岛素抵抗开始的低级系统炎症,进而可能导致 2 型糖尿病和认知能力下降。随着时间的推移,神经系统疾病已与老年疾病相关联。这种情况要求改变年轻一代的肥胖发生率。不健康的饮食消费和久坐不动的习惯可能会导致肠道对营养物质的吸收不良。一些植物和食物中的生物活性化合物可以减少或抑制威胁大脑防御系统的自由基清除剂、活性氧和金属离子复合物。啤酒花(Humulus lupulus L.)中的苦味酸已被证明对改善脂质和碳水化合物代谢有积极作用,并能通过α酸、β酸和类似物的作用减少炎症反应。因此,本研究旨在调查啤酒花苦味酸在肥胖和瘦小鼠体内的生物活性。为此,研究人员采用二氧化碳作为超临界萃取液,获得了干啤酒花提取物(DHE)。随后,称量78只C57BL/6J品系雄性小鼠的体重,并根据提供的饮食随机分为6组,每组13只:(NO)正常脂质饮食、(NO1)含0.35%α-酸的正常脂质饮食、(NO2)含3.5%α-酸的正常脂质饮食、(HP)高脂质饮食、(HP1)含0.35%α-酸的高脂质饮食和(HP2)含3.5%α-酸的高脂质饮食。在进行血糖耐受性和胰岛素耐受性测试后,与肥胖对照组(HP)相比,喂食 DHE 的动物(NO2 和 HP2)的血糖水平和体重增加的稳定性更好(P < 0.05)。通过检查 SOD 的酶谱,还观察到抗氧化能力有所改善;通过检查 GSK3β 和 p-IRS1 蛋白的表达,明显减轻了脑退化(p < 0.05)。为突显肥胖可能对动物大脑造成的伤害而进行的y-迷宫认知测试并未显示各组之间存在统计学差异。尽管肥胖 HP2 组(33.32 ± 4.11,p < 0.05)和对照组 HP(42.3 ± 5.88,p < 0.05)的每周饮食摄入量不同。DHE 中的生物活性化合物对血糖控制、胰岛素信号转导以及由此产生的肥胖相关标记物与大脑炎症进展的调节作用具有相关影响。
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引用次数: 0
In vitro gastrointestinal stability and intestinal absorption of ACE-1 and DPP4 inhibitory peptides from poultry by-product hydrolysates. 家禽副产品水解物中的 ACE-1 和 DPP4 抑制肽的体外胃肠道稳定性和肠道吸收。
IF 5.1 1区 农林科学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-07-15 DOI: 10.1039/d4fo01214c
Liudmila Sorokina, Nina Therese Solberg, Shiori Koga, Sissel Beate Rønning, Nils Kristian Afseth, Steven Ray Wilson, Anne Rieder, Sileshi Gizachew Wubshet

Bioactive peptides derived from food are promising health-promoting ingredients that can be used in functional foods and nutraceutical formulations. In addition to the potency towards the selected therapeutic target, the bioavailability of bioactive peptides is a major factor regarding clinical efficacy. We have previously shown that a low molecular weight peptide fraction (LMWPF) from poultry by-product hydrolysates possesses angiotensin-1-converting enzyme (ACE-1) and dipeptidyl-peptidase 4 (DPP4) inhibitory activities. The present study aimed to investigate the bioavailability of the bioactive peptides in the LMWPF. Prior to the investigation of bioavailability, a dipeptide YA was identified from this fraction as a dual inhibitor of ACE-1 and DPP4. Gastrointestinal (GI) stability and intestinal absorption of the bioactive peptides (i.e., YA as well as two previously reported bioactive dipeptides (VL and IY)) in the LMWPF were evaluated using the INFOGEST static in vitro digestion model and intestinal Caco-2 cell monolayer, respectively. Analysis of peptides after in vitro digestion confirmed that the dipeptides were resistant to the simulated GI conditions. After 4 hours of incubation, the concentration of the peptide from the apical side of the Caco-2 cell monolayer showed a significant decrease. However, the corresponding absorbed peptides were not detected on the basolateral side, suggesting that the peptides were not transported across the intestinal monolayer but rather taken up or metabolized by the Caco2 cells. Furthermore, when analyzing the gene expression of the Caco-2 cells upon peptide stimulation, a down-regulation of peptide transporters, the transcription factor CDX2, and the tight junction protein-1 (TJP1) was observed, suggesting the specific effects of the peptides on the Caco-2 cells. The study demonstrated that bioactive dipeptides found in the LMWPF were stable through in vitro GI digestion; however, the overall bioavailability may be hindered by inadequate uptake across the intestinal barrier.

从食物中提取的生物活性肽是很有前景的健康促进成分,可用于功能食品和营养保健品配方。除了对所选治疗靶点的效力外,生物活性肽的生物利用度也是影响临床疗效的一个重要因素。我们之前已经证明,从家禽副产品水解物中提取的低分子量肽组分(LMWPF)具有血管紧张素-1 转化酶(ACE-1)和二肽基肽酶 4(DPP4)抑制活性。本研究旨在调查 LMWPF 中生物活性肽的生物利用度。在研究生物利用度之前,从该馏分中发现了一种二肽 YA,它是 ACE-1 和 DPP4 的双重抑制剂。利用 INFOGEST 静态体外消化模型和肠道 Caco-2 细胞单层,分别评估了 LMWPF 中生物活性肽(即 YA 以及之前报道的两种生物活性二肽(VL 和 IY))的胃肠道(GI)稳定性和肠道吸收情况。体外消化后对肽的分析表明,二肽对模拟的消化条件具有抵抗力。培养 4 小时后,Caco-2 细胞单层顶端的肽浓度显著下降。然而,在基底侧并没有检测到相应被吸收的多肽,这表明多肽并没有通过肠道单层运输,而是被 Caco2 细胞吸收或代谢掉了。此外,在分析 Caco-2 细胞在多肽刺激下的基因表达时,观察到多肽转运体、转录因子 CDX2 和紧密连接蛋白-1(TJP1)下调,这表明多肽对 Caco-2 细胞有特殊作用。该研究表明,LMWPF 中的生物活性二肽在体外消化过程中是稳定的;但是,总体生物利用度可能会因肠道屏障吸收不足而受到影响。
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引用次数: 0
Macronutrient digestion and polyphenol bioaccessibility in oat milk tea products: an in vitro gastrointestinal tract study. 燕麦奶茶产品中的宏量营养素消化和多酚生物可及性:体外胃肠道研究。
IF 5.1 1区 农林科学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-07-15 DOI: 10.1039/d4fo01439a
Sirui Qin, Ruyi Li, David Julian McClements, Ying Chen, Zhihao Duan, Mianhong Chen, Yaping Dai, Liangkun Liao, Wei Zhou, Jihua Li

People are increasingly preparing milk tea using plant-based milks rather than cow's milk, e.g., vegans, those with lactose intolerance, and those with flavor preferences. However, adding plant-based milks to tea may impact the digestion, release, and bioaccessibility of nutrients and nutraceuticals in both the tea and milk. In this study, oat milk tea model systems (OMTMSs) containing different fat and tea polyphenol concentrations were used to explore the impact of tea on macronutrient digestion in oat milk, as well as the impact of oat milk matrix on the polyphenol bioaccessibility in the tea. An in vitro gastrointestinal model that mimics the mouth, stomach, and small intestine was used. Tea polyphenols (>0.25%) significantly reduced the glucose and free fatty acids released from oat milk after intestinal digestion. Tea polyphenols (>0.10%) also inhibited protein digestion in oat milk during gastric digestion but not during intestinal digestion. The bioaccessibility of the polyphenols in the tea depended on the fat content of oat milk, being higher for medium-fat (3.0%) and high-fat (5.8%) oat milk than low-fat (1.5%) oat milk. Liquid chromatography-tandem mass spectrometry (UPLC-ESI-MS/MS) analysis showed that lipids improved the tea polyphenol bioaccessibility by influencing the release of flavonoids and phenolic acids from the food matrices. These results provide important information about the impact of tea on the gastrointestinal fate of oat milk, and vice versa, which may be important for enhancing the healthiness of plant-based beverages.

越来越多的人开始使用植物奶而不是牛奶来制作奶茶,例如素食主义者、乳糖不耐症患者和有口味偏好的人。然而,在奶茶中添加植物奶可能会影响奶茶和牛奶中营养成分和营养保健品的消化、释放和生物可及性。本研究使用含有不同脂肪和茶多酚浓度的燕麦奶茶模型系统(OMTMSs)来探讨茶对燕麦奶中宏量营养素消化的影响,以及燕麦奶基质对茶中茶多酚生物可及性的影响。研究使用了一个模拟口腔、胃和小肠的体外胃肠道模型。茶多酚(>0.25%)能显著降低燕麦奶经肠道消化后释放的葡萄糖和游离脂肪酸。茶多酚(>0.10%)还能抑制燕麦奶在胃消化过程中对蛋白质的消化,但不能抑制肠道消化。茶多酚的生物可及性取决于燕麦奶的脂肪含量,中脂(3.0%)和高脂(5.8%)燕麦奶的生物可及性高于低脂(1.5%)燕麦奶。液相色谱-串联质谱(UPLC-ESI-MS/MS)分析表明,脂质通过影响类黄酮和酚酸从食物基质中的释放,提高了茶多酚的生物可及性。这些结果提供了有关茶对燕麦奶胃肠道转归影响的重要信息,反之亦然,这对于提高植物饮料的健康性可能非常重要。
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引用次数: 0
Resistant starch and tannic acid synergistically ameliorated dextran sulfate sodium-induced ulcerative colitis, particularly in the distal colon. 抗性淀粉和单宁酸协同改善了右旋糖酐硫酸钠诱发的溃疡性结肠炎,尤其是在远端结肠。
IF 5.1 1区 农林科学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-07-15 DOI: 10.1039/d4fo00531g
Zijun Liu, Shunjing Luo, Chengmei Liu, Xiuting Hu

We previously confirmed that tannic acid could delay the metabolism of resistant starch in vitro, which suggested that tannic acid might deliver resistant starch to the distal colon in vivo. Accordingly, co-supplementation of resistant starch and tannic acid might be beneficial for keeping the distal colon healthy. Thus, this study compared the effects of resistant starch, tannic acid and their mixtures on dextran sulfate sodium (DSS)-induced ulcerative colitis in mice. It was found that the mixtures had a more profound effect on ameliorating DSS-induced ulcerative colitis than resistant starch or tannic acid. In particular, the mixtures reversed the histology damage of the distal colon induced by DSS, while resistant starch or tannic acid alone did not. The mixtures also had a stronger ability to resist oxidative stress and inhibit inflammation in the distal colon. These results suggested that resistant starch and tannic acid synergistically alleviated DSS-induced ulcerative colitis, particularly in the distal colon. On the other hand, DSS decreased the production of short-chain fatty acids and induced significant microbial disorder, while the administration of resistant starch, tannic acid and their mixtures reversed the above shifts caused by DSS. In particular, the mixtures exhibited stronger prebiotic activity, as indicated by the microbial composition and production of short-chain fatty acids. Therefore, it was inferred that tannic acid delivered resistant starch to the distal colon of mice, and thus the mixtures had stronger prebiotic activity. As a result, the mixtures effectively alleviated ulcerative colitis in the whole colon.

我们之前证实,单宁酸可延缓抗性淀粉在体外的代谢,这表明单宁酸可在体内将抗性淀粉输送到远端结肠。因此,同时补充抗性淀粉和单宁酸可能有利于保持远端结肠的健康。因此,本研究比较了抗性淀粉、单宁酸及其混合物对葡聚糖硫酸钠(DSS)诱导的小鼠溃疡性结肠炎的影响。研究发现,与抗性淀粉或单宁酸相比,混合物对改善右旋糖酐硫酸钠诱发的溃疡性结肠炎有更深远的影响。特别是,混合物能逆转 DSS 诱导的远端结肠组织学损伤,而单独使用抗性淀粉或单宁酸则不能。混合物还具有更强的抗氧化应激和抑制远端结肠炎症的能力。这些结果表明,抗性淀粉和单宁酸协同缓解了 DSS 诱导的溃疡性结肠炎,尤其是在远端结肠。另一方面,DSS 减少了短链脂肪酸的产生,并诱发了严重的微生物紊乱,而抗性淀粉、单宁酸及其混合物的施用则逆转了 DSS 引起的上述变化。特别是,从微生物组成和短链脂肪酸的产生情况来看,混合物表现出更强的益生元活性。因此,可以推断单宁酸向小鼠的远端结肠输送了抗性淀粉,因此混合物具有更强的益生元活性。因此,混合物能有效缓解全结肠的溃疡性结肠炎。
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引用次数: 0
Lower plasma linoleic acids as a risk factor for gout: an integrated analysis of population-based cohort and genetic data. 较低的血浆亚油酸是痛风的风险因素:基于人群的队列和遗传数据的综合分析。
IF 5.1 1区 农林科学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-07-15 DOI: 10.1039/d4fo00987h
Hao-Wei Tao, Zhong-Yue Liu, Wei Jiang, Meng-Yuan Miao, Jie-Qiong Lyu, Miao Zhao, Jie Zhu, Jin-Si Chen, Hong-Zhen Du, Zeng-Ning Li, Zhengbao Zhu, Jing Yang, Li-Qiang Qin, Wei Chen, Guo-Chong Chen

Background: Gout is a nutrition-related, highly prevalent inflammatory arthritis with undesirable effects on the quality of life. The relationships between circulating fatty acids (FAs) and gout remain poorly understood. Method: We included 268 174 participants with plasma FAs measured using nuclear magnetic resonance at the baseline (2006-2010) from the UK Biobank, of which 15 194 participants had repeated measures of FAs between 2012 and 2013. Cox proportional hazards models were used to assess the association of the baseline and longitudinal changes in relative levels of plasma FAs (% total FAs) with incident gout. Mendelian randomization (MR) analyses were conducted to assess the potential causality of the examined association. Results: Over a median follow-up of 12.8 years, 5160 incident cases of gout occurred. Baseline polyunsaturated fatty acids (PUFAs), n-6 PUFAs, and linoleic acids (LAs) were inversely associated with incident gout (all P-trend values < 0.0001). Baseline monounsaturated fatty acids (MUFAs), n-3 PUFAs, and docosahexaenoic acids (DHAs) were positively associated with incident gout (all P-trend values < 0.0001). Longitudinal increments of n-6 PUFAs and LAs were associated with a lower risk of subsequent gout, whereas an increment of n-3 PUFAs was associated with a higher risk. In two-sample MR analyses, genetically determined higher levels of PUFAs, n-6 PUFAs, and LAs were associated with a decreased risk of gout (all P values < 0.05). Conclusions: Our findings consistently indicate a causal relationship of elevated levels of n-6 PUFAs, especially LAs, with a reduced risk of gout.

背景:痛风是一种与营养有关的高发炎症性关节炎,对生活质量有不良影响。人们对循环脂肪酸(FA)与痛风之间的关系仍然知之甚少。研究方法我们纳入了英国生物库中 268 174 名在基线(2006-2010 年)使用核磁共振测量血浆脂肪酸的参与者,其中 15 194 名参与者在 2012 年至 2013 年期间重复测量了脂肪酸。采用Cox比例危险模型评估血浆脂肪酸相对水平(总脂肪酸百分比)的基线和纵向变化与痛风发病率的关系。还进行了孟德尔随机化(MR)分析,以评估所研究关联的潜在因果关系。研究结果中位随访时间为 12.8 年,共发现 5160 例痛风病例。基线多不饱和脂肪酸(PUFAs)、n-6 PUFAs和亚油酸(LAs)与痛风的发病率成反比(所有P趋势值均小于0.0001)。基线单不饱和脂肪酸(MUFAs)、n-3 PUFAs和二十二碳六烯酸(DHAs)与痛风发病率呈正相关(所有P趋势值均小于0.0001)。n-6 PUFAs 和 LAs 的纵向增加与痛风发生风险的降低有关,而 n-3 PUFAs 的增加与痛风发生风险的升高有关。在双样本 MR 分析中,由基因决定的较高水平的 PUFAs、n-6 PUFAs 和 LAs 与痛风风险的降低有关(所有 P 值均小于 0.05)。结论:我们的研究结果一致表明,n-6 PUFAs(尤其是 LAs)水平升高与痛风风险降低之间存在因果关系。
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引用次数: 0
Porphyra haitanensis glycoprotein regulates glucose homeostasis: targeting the liver. Porphyra haitanensis 糖蛋白调节葡萄糖稳态:以肝脏为目标。
IF 5.1 1区 农林科学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-07-15 DOI: 10.1039/d4fo01544d
Yujia Ou, Yuehong Guo, Haoyu Wang, Zebin Guo, Baodong Zheng

In this study, we investigated the effects of glycoprotein (PG)-mediated regulation of Porphyra haitanensis on liver glucose metabolism in hyperglycemic mouse models, and sought to establish the underlying mechanism, as determined by the changes in liver gene expression and metabolic profiles. The results showed that 30-300 mg kg-1 PG upregulated the expression of the liver genes Ins1, Ins2, Insr, Gys2, Gpi1, Gck, and downregulated the expression of G6pc, G6pc2, and G6pc3, in a concentration-dependent manner. 300 mg kg-1 PG downregulated the concentrations of glucose-related metabolites in the liver, but upregulated lactic acid, 2-aminoacetic acid, and glucose-1-phosphate concentrations. It was assumed that PG regulated liver glucose metabolism by enriching insulin secretion, glycolysis/gluconeogenesis, and the AMPK signaling pathway, and promoting insulin secretion, glycogen synthesis, and glycolysis. Our findings supported the development of P. haitanensis and its glycoproteins as novel natural antidiabetic compounds that regulated blood glucose homeostasis.

本研究探讨了由糖蛋白(PG)介导的海腥草对高血糖小鼠模型肝糖代谢的调控作用,并试图通过肝脏基因表达和代谢谱的变化来确定其潜在机制。结果表明,30-300 毫克/千克-1 PG 能上调肝脏基因 Ins1、Ins2、Insr、Gys2、Gpi1、Gck 的表达,下调 G6pc、G6pc2 和 G6pc3 的表达,且呈浓度依赖性。300 mg kg-1 PG 下调了肝脏中葡萄糖相关代谢物的浓度,但上调了乳酸、2-氨基乙酸和葡萄糖-1-磷酸的浓度。据推测,PG 通过丰富胰岛素分泌、糖酵解/糖原生成和 AMPK 信号通路,以及促进胰岛素分泌、糖原合成和糖酵解来调节肝脏葡萄糖代谢。我们的研究结果支持将海金沙及其糖蛋白开发为新型天然抗糖尿病化合物,以调节血糖平衡。
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引用次数: 0
Urolithin B protects mice from diet-induced obesity, insulin resistance, and intestinal inflammation by regulating gut microbiota composition. 尿石素 B 通过调节肠道微生物群的组成,保护小鼠免受饮食引起的肥胖、胰岛素抵抗和肠道炎症的影响。
IF 5.1 1区 农林科学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-07-15 DOI: 10.1039/d4fo02545h
Peng Chen, Rong Wang, Jiexin Lei, Lihua Feng, Benhong Zhou

The increasing prevalence of obesity and type 2 diabetes (T2D) signifies the failure of conventional treatments for these diseases. The gut microbiota has been proposed as a key player in the pathophysiology of diet-induced T2D. Urolithin B (Uro B), a gut microbiota-derived polyphenol metabolite, exerts several beneficial health effects. In this study, we investigated the metabolic effects of Uro B on high-fat/high-sucrose (HFHS)-fed mice and determined whether its antidiabetic effects are related to the modulation of the gut microbiota. C57BL/6J mice were fed either a chow or HFHS diet. HFHS-fed mice were administered daily with either a vehicle (water) or different doses of Uro B (100 or 200 mg kg-1) for eight weeks. The composition of the gut microbiota was assessed by 16S rRNA sequencing. The results showed that Uro B treatment reduced HFHS-induced weight gain and visceral obesity and decreased liver weight and triglyceride accumulation associated with blunted hepatic oxidative stress and inflammation. Furthermore, Uro B administration improved insulin sensitivity as revealed by improved insulin tolerance, a lower homeostasis model assessment of insulin resistance, and decreased glucose-induced hyperinsulinemia during the oral glucose tolerance test. Uro B treatment was found to lower the intestinal triglyceride content and alleviate intestinal inflammation and oxidative stress. Remarkably, Uro B treatment markedly increased the proportion of the mucin-degrading bacterium Akkermansia in metagenomic samples. In conclusion, Uro B exerts beneficial metabolic effects by alleviating HFHS diet-induced features of metabolic syndrome, which is associated with a proportional increase in the population of Akkermansia spp.

肥胖症和 2 型糖尿病(T2D)发病率的不断上升表明,治疗这些疾病的传统方法已经失效。肠道微生物群被认为是饮食诱发 T2D 的病理生理学中的关键角色。尿石素 B(Uro B)是一种源于肠道微生物群的多酚代谢物,具有多种有益健康的作用。在这项研究中,我们调查了尿石素 B 对高脂/高蔗糖(HFHS)喂养小鼠的代谢影响,并确定其抗糖尿病作用是否与肠道微生物群的调节有关。C57BL/6J 小鼠以饲料或 HFHS 为食。喂食 HFHS 的小鼠每天服用载体(水)或不同剂量的 Uro B(100 或 200 mg kg-1),连续服用八周。通过 16S rRNA 测序评估了肠道微生物群的组成。结果表明,Uro B治疗可减少HFHS诱发的体重增加和内脏肥胖,降低肝脏重量和甘油三酯累积,同时减轻肝脏氧化应激和炎症反应。此外,胰岛素耐受性的改善、胰岛素抵抗平衡模型评估值的降低以及口服葡萄糖耐量试验中葡萄糖诱导的高胰岛素血症的减少都表明,服用 Uro B 提高了胰岛素敏感性。研究发现 Uro B 治疗可降低肠道甘油三酯含量,缓解肠道炎症和氧化应激。值得注意的是,Uro B 处理明显增加了元基因组样本中粘蛋白降解菌 Akkermansia 的比例。总之,Uro B 通过缓解 HFHS 膳食诱发的代谢综合征特征而对代谢产生有益影响,而代谢综合征特征与 Akkermansia 菌群的比例增加有关。
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引用次数: 0
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Food & Function
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