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Classification of 3D Multicellular Organization in Phase Microscopy for High Throughput Screening of Therapeutic Targets. 用于高通量筛选治疗靶点的三维多细胞组织的相显微镜分类。
Pub Date : 2015-01-01 DOI: 10.1109/WACV.2015.64
Hang Chang, Bahram Parvin

The current trend in high throughput screening is the utilization of more complex model systems that mimic both structural and functional properties of cellular processes in vivo. In this context, 3D cell culture models have emerged as effective systems to study tumor initiation and cancer behavior, where colony organization represents distinct phenotypic signatures that enable differentiation of cancer cells in culture using phase imaging and in the absence of clinical markers. If the colony organization can be classified into different phenotypes, it will enable rapid drug screening using phase microscopy. In this paper, we propose a novel method based on locality-constrained dictionary learning for the discrimination of aberrant colony organization in phase images, which encodes original SIFT (Scale-Invariant Feature Transform) features into high dimensional sparse codes with locality-preserving landmark points on the nonlinear manifold, and summarizes the sparse features at various locations and scales through spatial pyramid matching for robust representation. Experimental results demonstrate the significant improvement of performance, compared to the state-of-art in the field.

目前高通量筛选的趋势是利用更复杂的模型系统来模拟体内细胞过程的结构和功能特性。在这种情况下,3D细胞培养模型已经成为研究肿瘤起始和癌症行为的有效系统,其中集落组织代表了不同的表型特征,可以在缺乏临床标志物的情况下使用相成像技术在培养中区分癌细胞。如果菌落组织可以分为不同的表型,它将使快速药物筛选使用相显微镜。本文提出了一种基于位置约束字典学习的相位图像异常群体组织判别方法,该方法将原始SIFT特征编码为非线性流形上具有位置保持特征点的高维稀疏编码,并通过空间金字塔匹配对不同位置和尺度上的稀疏特征进行汇总,实现鲁棒表示。实验结果表明,与该领域的最新技术相比,该系统的性能有了显著提高。
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引用次数: 1
Image Alignment for Multiple Camera High Dynamic Range Microscopy. 多相机高动态范围显微镜的图像对齐。
Pub Date : 2012-01-09 DOI: 10.1109/WACV.2012.6163015
Brian S Eastwood, Elisabeth C Childs

This paper investigates the problem of image alignment for multiple camera high dynamic range (HDR) imaging. HDR imaging combines information from images taken with different exposure settings. Combining information from multiple cameras requires an alignment process that is robust to the intensity differences in the images. HDR applications that use a limited number of component images require an alignment technique that is robust to large exposure differences. We evaluate the suitability for HDR alignment of three exposure-robust techniques. We conclude that image alignment based on matching feature descriptors extracted from radiant power images from calibrated cameras yields the most accurate and robust solution. We demonstrate the use of this alignment technique in a high dynamic range video microscope that enables live specimen imaging with a greater level of detail than can be captured with a single camera.

研究了多相机高动态范围(HDR)成像中的图像对准问题。HDR成像结合了不同曝光设置下拍摄的图像信息。组合来自多个相机的信息需要一个对图像强度差异具有鲁棒性的校准过程。使用有限数量的组件图像的HDR应用程序需要一种对大曝光差具有鲁棒性的校准技术。我们评估了三种曝光稳健技术对HDR校准的适用性。我们得出结论,基于从校准相机的辐射功率图像中提取的匹配特征描述符的图像对齐产生最准确和鲁棒的解决方案。我们演示了在高动态范围视频显微镜中使用这种对准技术,该技术可以使活体标本成像具有比单个摄像机更大的细节水平。
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引用次数: 0
Localizing Blurry and Low-Resolution Text in Natural Images. 在自然图像中定位模糊和低分辨率文本。
Pub Date : 2011-02-10 DOI: 10.1109/WACV.2011.5711546
Pannag Sanketi, Huiying Shen, James M Coughlan

There is a growing body of work addressing the problem of localizing printed text regions occurring in natural scenes, all of it focused on images in which the text to be localized is resolved clearly enough to be read by OCR. This paper introduces an alternative approach to text localization based on the fact that it is often useful to localize text that is identifiable as text but too blurry or small to be read, for two reasons. First, an image can be decimated and processed at a coarser resolution than usual, resulting in faster localization before OCR is performed (at full resolution, if needed). Second, in real-time applications such as a cell phone app to find and read text, text may initially be acquired from a lower-resolution video image in which it appears too small to be read; once the text's presence and location have been established, a higher-resolution image can be taken in order to resolve the text clearly enough to read it.We demonstrate proof of concept of this approach by describing a novel algorithm for binarizing the image and extracting candidate text features, called "blobs," and grouping and classifying the blobs into text and non-text categories. Experimental results are shown on a variety of images in which the text is resolved too poorly to be clearly read, but is still identifiable by our algorithm as text.

有越来越多的工作解决了在自然场景中出现的打印文本区域的本地化问题,所有这些工作都集中在需要本地化的文本足够清晰地被OCR读取的图像上。本文介绍了文本本地化的另一种方法,基于这样一个事实,即本地化那些可识别为文本但太模糊或太小而无法阅读的文本通常很有用,原因有两个。首先,图像可以在比通常更粗的分辨率下进行抽取和处理,从而在OCR执行之前更快地定位(如果需要,可以在全分辨率下)。第二,在实时应用程序中,例如查找和读取文本的手机应用程序,文本最初可能从分辨率较低的视频图像中获取,其中文本看起来太小而无法读取;一旦确定了文本的存在和位置,就可以拍摄更高分辨率的图像,以便清晰地解析文本以进行阅读。我们通过描述一种用于图像二值化和提取候选文本特征(称为“blob”)的新算法,并将blob分组和分类为文本和非文本类别,来证明这种方法的概念。实验结果显示在各种图像中,其中的文本分辨率太低,无法清晰阅读,但仍然可以被我们的算法识别为文本。
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引用次数: 0
Real-Time Detection and Reading of LED/LCD Displays for Visually Impaired Persons. 为视障人士实时检测和读取 LED/LCD 显示屏。
Pub Date : 2011-01-05 DOI: 10.1109/WACV.2011.5711544
Ender Tekin, James M Coughlan, Huiying Shen

Modern household appliances, such as microwave ovens and DVD players, increasingly require users to read an LED or LCD display to operate them, posing a severe obstacle for persons with blindness or visual impairment. While OCR-enabled devices are emerging to address the related problem of reading text in printed documents, they are not designed to tackle the challenge of finding and reading characters in appliance displays. Any system for reading these characters must address the challenge of first locating the characters among substantial amounts of background clutter; moreover, poor contrast and the abundance of specular highlights on the display surface - which degrade the image in an unpredictable way as the camera is moved - motivate the need for a system that processes images at a few frames per second, rather than forcing the user to take several photos, each of which can take seconds to acquire and process, until one is readable.We describe a novel system that acquires video, detects and reads LED/LCD characters in real time, reading them aloud to the user with synthesized speech. The system has been implemented on both a desktop and a cell phone. Experimental results are reported on videos of display images, demonstrating the feasibility of the system.

微波炉和 DVD 播放器等现代家用电器越来越多地要求用户通过阅读 LED 或 LCD 显示屏来进行操作,这给失明或视力受损者造成了严重障碍。虽然支持 OCR 的设备正在兴起,以解决阅读印刷文件中文本的相关问题,但它们并不是为解决查找和阅读家电显示屏中的字符这一难题而设计的。任何读取这些字符的系统都必须首先解决在大量杂乱背景中定位字符的难题;此外,显示屏表面对比度差和大量镜面高光(随着摄像头的移动,图像会以不可预测的方式衰减)促使我们需要一个能以每秒几帧的速度处理图像的系统,而不是强迫用户拍摄多张照片,每张照片的获取和处理都需要几秒钟的时间,直到其中一张可以读取为止。我们介绍了一种新型系统,该系统可实时获取视频、检测和读取 LED/LCD 字符,并通过合成语音向用户朗读这些字符。该系统已在台式机和手机上实现。报告了显示图像视频的实验结果,证明了该系统的可行性。
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引用次数: 0
Groupwise Pose Normalization for Craniofacial Applications. 颅面应用分组位姿归一化。
Pub Date : 2011-01-01 DOI: 10.1109/WACV.2011.5711510
Jiun-Hung Chen, Linda G Shapiro

A general framework is proposed for solving groupwise pose normalization problems and is analyzed in detail under different feature spaces. The analysis shows that using principal component analysis for pose normalization is a special case of using the proposed framework under a special feature space. The experimental results on two craniofacial datasets show the proposed method achieved promising results for solving groupwise pose normalization problems for craniofacial applications.

提出了求解群姿态归一化问题的一般框架,并对不同特征空间下群姿态归一化问题进行了详细分析。分析表明,利用主成分分析进行位姿归一化是在特定特征空间下使用该框架的一种特殊情况。在两个颅面数据集上的实验结果表明,该方法在解决颅面应用中分组姿态归一化问题方面取得了良好的效果。
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引用次数: 2
An Algorithm Enabling Blind Users to Find and Read Barcodes. 一种使盲人用户能够查找和读取条形码的算法。
Pub Date : 2009-12-07 DOI: 10.1109/WACV.2009.5403098
Ender Tekin, James M Coughlan

Most camera-based systems for finding and reading barcodes are designed to be used by sighted users (e.g. the Red Laser iPhone app), and assume the user carefully centers the barcode in the image before the barcode is read. Blind individuals could benefit greatly from such systems to identify packaged goods (such as canned goods in a supermarket), but unfortunately in their current form these systems are completely inaccessible because of their reliance on visual feedback from the user.To remedy this problem, we propose a computer vision algorithm that processes several frames of video per second to detect barcodes from a distance of several inches; the algorithm issues directional information with audio feedback (e.g. "left," "right") and thereby guides a blind user holding a webcam or other portable camera to locate and home in on a barcode. Once the barcode is detected at sufficiently close range, a barcode reading algorithm previously developed by the authors scans and reads aloud the barcode and the corresponding product information. We demonstrate encouraging experimental results of our proposed system implemented on a desktop computer with a webcam held by a blindfolded user; ultimately the system will be ported to a camera phone for use by visually impaired users.

大多数用于查找和读取条形码的基于摄像头的系统都是为视力正常的用户设计的(例如Red Laser iPhone应用程序),并假设用户在读取条形码之前仔细地将条形码放在图像的中心。盲人可以从这种识别包装商品(如超市里的罐头商品)的系统中受益匪浅,但不幸的是,在目前的形式下,这些系统完全无法使用,因为它们依赖于用户的视觉反馈。为了解决这个问题,我们提出了一种计算机视觉算法,该算法每秒处理几帧视频以检测几英寸远的条形码;该算法通过音频反馈发出定向信息(例如:“左”、“右”),从而引导手持网络摄像头或其他便携式摄像头的盲人用户定位并锁定条形码。一旦在足够近的范围内检测到条形码,作者先前开发的条形码读取算法就会扫描并大声读出条形码和相应的产品信息。我们展示了我们提出的系统在台式计算机上实现的令人鼓舞的实验结果,该系统由蒙眼用户持有网络摄像头;最终,该系统将被移植到一部手机上,供视障用户使用。
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引用次数: 0
Reading Challenging Barcodes with Cameras. 用相机读取具有挑战性的条形码。
Pub Date : 2009-12-01 DOI: 10.1109/WACV.2009.5403090
Orazio Gallo, Roberto Manduchi

Current camera-based barcode readers do not work well when the image has low resolution, is out of focus, or is motion-blurred. One main reason is that virtually all existing algorithms perform some sort of binarization, either by gray scale thresholding or by finding the bar edges. We propose a new approach to barcode reading that never needs to binarize the image. Instead, we use deformable barcode digit models in a maximum likelihood setting. We show that the particular nature of these models enables efficient integration over the space of deformations. Global optimization over all digits is then performed using dynamic programming. Experiments with challenging UPC-A barcode images show substantial improvement over other state-of-the-art algorithms.

当前基于摄像头的条形码读取器在图像分辨率低、失焦或运动模糊时不能很好地工作。一个主要原因是,几乎所有现有的算法都执行某种二值化,要么是通过灰度阈值,要么是通过寻找条形边缘。我们提出了一种新的条形码读取方法,它不需要对图像进行二值化。相反,我们在最大似然设置中使用可变形的条形码数字模型。我们证明了这些模型的特殊性质使得在变形空间上的有效集成成为可能。然后使用动态规划对所有数字进行全局优化。具有挑战性的UPC-A条形码图像的实验表明,与其他最先进的算法相比,有了实质性的改进。
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引用次数: 0
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Proceedings. IEEE Workshop on Applications of Computer Vision
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