Pub Date : 2025-12-01DOI: 10.1016/j.jfutfo.2025.12.006
Jiahui Zhu, Shilong Lv, Mo-Lin Li, W. Zhu, Yushen Liu, Lu‐Liang Wang
• A novel core-shell magnetic covalent organic framework (Fe 3 O 4 @TZ-DMTP) was synthesized via in situ hydrothermal growth. • Effective extraction performance for ornidazole was achieved by π-π, hydrogen bonding and hydrophobic interaction sites of Fe 3 O 4 @TZ-DMTP adsorbent . • The established Fe 3 O 4 @TZ-DMTP-MSPE-HPLC method for ornidazole was validated and applied in real food samples analysis. Ornidazole (ONZ), a widely used nitroimidazole antibiotic, has raised significant environmental and health concerns due to its persistence and potential carcinogenicity. Existing detection methods often suffer from matrix interference and cumbersome procedures. In this study, a novel magnetic covalent organic framework (Fe 3 O 4 @TZ-DMTP) with a core-shell structure was synthesized via in situ hydrothermal growth of 2,4,6-tri(4-aminophenyl)-1,3,5-triazine (TZ) and 2,5-dimethoxy-terephthalaldehyde (DMTP) on magnetic nanoparticles. The composite exhibited high surface area, superparamagnetism and excellent adsorption capacity for ONZ through π-π interactions, hydrogen bonding, and hydrophobic effects. Coupled with HPLC-DAD, a robust magnetic solid-phase extraction (MSPE) method was developed, achieving a wide linear range (0.01-10 µg mL⁻¹) and low detection limit (0.003 µg mL⁻¹). The method demonstrated superior anti-interference capability and reusability (>90% recovery after 7 cycles). Successful application to tap water, lake water, aquaculture water, milk and honey samples confirmed its practicality with recoveries of 80.8-103.2% and RSDs < 13.8%. This work provides a sustainable and efficient strategy for monitoring nitroimidazole residues in complex matrices.
•通过原位水热生长合成了一种新型核壳磁性共价有机骨架（fe3o4 @TZ-DMTP）。•fe3o4 @TZ-DMTP吸附剂的π-π、氢键和疏水相互作用位点对奥硝唑的萃取效果较好。验证了建立的Fe 3 O 4 @TZ-DMTP-MSPE-HPLC检测奥硝唑的方法，并将其应用于实际食品样品的分析。奥硝唑（Ornidazole， ONZ）是一种广泛使用的硝基咪唑类抗生素，由于其持久性和潜在的致癌性，引起了严重的环境和健康问题。现有的检测方法往往存在矩阵干扰和繁琐的程序。在本研究中，通过在磁性纳米颗粒上原位水热生长，合成了具有核壳结构的新型磁性共价有机骨架（Fe 3 O 4 @TZ-DMTP）。该复合材料通过π-π相互作用、氢键和疏水效应对ONZ具有高表面积、超顺磁性和优异的吸附能力。与HPLC-DAD相结合，建立了一种可靠的磁固相萃取（MSPE）方法，具有宽的线性范围（0.01-10µg mL⁻¹）和低的检出限（0.003µg mL⁻¹）。该方法具有良好的抗干扰能力和可重复使用性（循环7次后回收率高达90%）。成功应用于自来水、湖水、水产养殖水、牛奶和蜂蜜样品，回收率为80.8 ~ 103.2%,rsd < 13.8%，证实了该方法的实用性。这项工作为监测复杂基质中硝基咪唑残留提供了一种可持续和有效的策略。
{"title":"Facile Synthesis of Magnetic Covalent Organic Frameworks for Highly Efficient Extraction and Trace Determination of Ornidazole in Food Samples","authors":"Jiahui Zhu, Shilong Lv, Mo-Lin Li, W. Zhu, Yushen Liu, Lu‐Liang Wang","doi":"10.1016/j.jfutfo.2025.12.006","DOIUrl":"https://doi.org/10.1016/j.jfutfo.2025.12.006","url":null,"abstract":"• A novel core-shell magnetic covalent organic framework (Fe 3 O 4 @TZ-DMTP) was synthesized via in situ hydrothermal growth. • Effective extraction performance for ornidazole was achieved by π-π, hydrogen bonding and hydrophobic interaction sites of Fe 3 O 4 @TZ-DMTP adsorbent . • The established Fe 3 O 4 @TZ-DMTP-MSPE-HPLC method for ornidazole was validated and applied in real food samples analysis. Ornidazole (ONZ), a widely used nitroimidazole antibiotic, has raised significant environmental and health concerns due to its persistence and potential carcinogenicity. Existing detection methods often suffer from matrix interference and cumbersome procedures. In this study, a novel magnetic covalent organic framework (Fe 3 O 4 @TZ-DMTP) with a core-shell structure was synthesized via in situ hydrothermal growth of 2,4,6-tri(4-aminophenyl)-1,3,5-triazine (TZ) and 2,5-dimethoxy-terephthalaldehyde (DMTP) on magnetic nanoparticles. The composite exhibited high surface area, superparamagnetism and excellent adsorption capacity for ONZ through π-π interactions, hydrogen bonding, and hydrophobic effects. Coupled with HPLC-DAD, a robust magnetic solid-phase extraction (MSPE) method was developed, achieving a wide linear range (0.01-10 µg mL⁻¹) and low detection limit (0.003 µg mL⁻¹). The method demonstrated superior anti-interference capability and reusability (>90% recovery after 7 cycles). Successful application to tap water, lake water, aquaculture water, milk and honey samples confirmed its practicality with recoveries of 80.8-103.2% and RSDs < 13.8%. This work provides a sustainable and efficient strategy for monitoring nitroimidazole residues in complex matrices.","PeriodicalId":100784,"journal":{"name":"Journal of Future Foods","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147333083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-11-01Epub Date: 2025-01-29DOI: 10.1016/j.jfutfo.2024.11.009
Yifeng Lu , Jing Li , Zihao He , Linyun Chen , Huixin Tian , Chen Xu , Xinglian Xu , Minyi Han
Changes in the quality of eggs during storage relate to their shelf life and economic value. Factors such as temperature, relative humidity, the operation of cleaning, and microorganisms have been shown to play a role in the storage quality of eggs. This study thus aimed at investigating the effect of hen age on the storage quality of egg, and predicting egg shelf life using back propagation artificial neural network (BP-ANN) based models. Eggs laid by Jingfen No.1 (27 and 58 weeks of age) and Jingfen No.6 (26 and 57 weeks of age) hens were stored under ambient conditions and evaluated by physicochemical properties. It was found that the shelf life of the lower age group was significantly longer than that of the higher age group. A novel hybrid model combining BP-ANN, cuckoo search and adaptive boosting (CS-BP-AdaBoost) was proposed for predicting the remaining egg shelf life, with the input being Haugh unit, yolk index, air cell depth, albumen pH, hen age, and breed. The tuning process of hyperparameters such as learning rate, training function, and transfer function was presented in detail. Results show that CS-BP-AdaBoost had satisfactory performance on the test set with root mean square error (RMSE) and coefficient of determination (R2) of 0.68 and 0.97, respectively. And it outperformed BP-ANN by reducing RMSE by 0.39 and improving R2 by 0.05. The model used solved the problem that the traditional BP-ANN tends to fall into local minima. The removal of hen age from the input parameters caused a decrease in prediction accuracy (R2 = 0.95, RMSE = 1.00), suggesting an important role of hen age in shelf life prediction. This study demonstrates the great potential of applying combinatorial modeling approaches to predict egg shelf life and the crucial impact of hen age on egg shelf life prediction.
{"title":"Egg freshness during storage: the effect of laying hen age and shelf life prediction using a novel hybrid modeling method","authors":"Yifeng Lu , Jing Li , Zihao He , Linyun Chen , Huixin Tian , Chen Xu , Xinglian Xu , Minyi Han","doi":"10.1016/j.jfutfo.2024.11.009","DOIUrl":"10.1016/j.jfutfo.2024.11.009","url":null,"abstract":"<div><div>Changes in the quality of eggs during storage relate to their shelf life and economic value. Factors such as temperature, relative humidity, the operation of cleaning, and microorganisms have been shown to play a role in the storage quality of eggs. This study thus aimed at investigating the effect of hen age on the storage quality of egg, and predicting egg shelf life using back propagation artificial neural network (BP-ANN) based models. Eggs laid by Jingfen No.1 (27 and 58 weeks of age) and Jingfen No.6 (26 and 57 weeks of age) hens were stored under ambient conditions and evaluated by physicochemical properties. It was found that the shelf life of the lower age group was significantly longer than that of the higher age group. A novel hybrid model combining BP-ANN, cuckoo search and adaptive boosting (CS-BP-AdaBoost) was proposed for predicting the remaining egg shelf life, with the input being Haugh unit, yolk index, air cell depth, albumen pH, hen age, and breed. The tuning process of hyperparameters such as learning rate, training function, and transfer function was presented in detail. Results show that CS-BP-AdaBoost had satisfactory performance on the test set with root mean square error (RMSE) and coefficient of determination (<em>R</em><sup>2</sup>) of 0.68 and 0.97, respectively. And it outperformed BP-ANN by reducing RMSE by 0.39 and improving <em>R</em><sup>2</sup> by 0.05. The model used solved the problem that the traditional BP-ANN tends to fall into local minima. The removal of hen age from the input parameters caused a decrease in prediction accuracy (<em>R</em><sup>2</sup> = 0.95, RMSE = 1.00), suggesting an important role of hen age in shelf life prediction. This study demonstrates the great potential of applying combinatorial modeling approaches to predict egg shelf life and the crucial impact of hen age on egg shelf life prediction.</div></div>","PeriodicalId":100784,"journal":{"name":"Journal of Future Foods","volume":"5 6","pages":"Pages 614-627"},"PeriodicalIF":5.2,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143104291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-11-01Epub Date: 2025-01-29DOI: 10.1016/j.jfutfo.2024.11.007
Yichen Liu , Arome Solomon Odiba , Qi Yu , Jin Xie , Nkwachukwu Oziamara Okoro , Guiyan Liao , Xinchen Zhu , Bin He , Cheng Jin , Wenxia Fang , Bin Wang
Due to the increase in aging populations and the prevalence of aging-associated diseases, there is a growing interest in finding therapeutic interventions. Antioxidants play a vital role in mitigating the adverse conditions associated with aging. In this study, we investigated the impact of Dendrobium officinale alkaloids (DOA) cultivated in diverse environments on antioxidative and anti-aging responses in Caenorhabditis elegans. Three distinct sources of DOA, represented as tree (TR)-DOA, greenhouse (GH)-DOA, and rock (RK)-DOA, were examined. Following initial testing of three DOA concentrations, 10 µg/mL was selected, which increased the TJ1060 lifespan by 23%, and exhibited no toxicity. TR-DOA, GH-DOA, and RK-DOA exhibited robust antioxidative effects, significantly reducing reactive oxygen species levels by 37%, 54%, and 60%, respectively on day 1, and 68%, 73%, and 75% on day 5, respectively. On day 4, TR-DOA, GH-DOA, and RK-DOA significantly reduced lipofuscin levels by 36%, 51%, and 39%, respectively (P < 0.000 1). On day 8, lipofuscin levels were significantly reduced by 34% (P < 0.01), 32% (P < 0.05), and 33% (P < 0.05), respectively. TR-DOA, GH-DOA, and RK-DOA, likewise, reduced the level of the endoplasmic reticulum stress marker, HSP-4::GFP, by 36%, 37%, and 35%, respectively (P < 0.000 1) on day 1, and by 38%, 40%, and 45%, respectively (P < 0.000 1) on day 8. Compared to the control, RK-DOA, GH-DOA, and TR-DOA significantly upregulated HSP-6::GFP (P < 0.0001, P < 0.001, and P < 0.05, respectively), and enhanced heat stress resistance (P < 0.0001, P < 0.001, and P < 0.01, respectively). RK-DOA, GH-DOA, and TR-DOA significantly reduced α-synuclein aggregation by 31%, 25%, and 18% respectively on day 1 (P < 0.0001, P < 0.0001, and P < 0.01, respectively), and by 32%, 27%, and 12% on day 3 (P < 0.01, P < 0.01, and P > 0.05, respectively). RK-DOA, GH-DOA, and TR-DOA delayed paralysis (P < 0.0001, in all cases) and significantly increased worm activity (P < 0.001, P < 0.001, and P < 0.01, respectively). RK-DOA, GH-DOA, and TR-DOA also significantly increased the rate of body bend on day 5 (P < 0.001, P < 0.01, P < 0.0001) and day 10 (P < 0.001, P < 0.0001, P < 0.01). Our findings suggest that DOA, particularly RK-DOA, offers promising antioxidative and anti-aging benefits in C. elegans. The differential responses among DOA variants highlight the importance of the cultivation environment in shaping the bioactivity of natural compounds.
由于老龄化人口的增加和与衰老有关的疾病的流行，人们对寻找治疗干预措施越来越感兴趣。抗氧化剂在减轻与衰老有关的不利条件方面起着至关重要的作用。本文研究了不同环境培养的铁皮石斛生物碱（DOA）对秀丽隐杆线虫抗氧化和抗衰老反应的影响。研究了三种不同的DOA来源，分别为树木(TR)-DOA、温室(GH)-DOA和岩石(RK)-DOA。在初始测试三种DOA浓度后，选择10µg/mL， TJ1060寿命延长23%，且无毒性。TR-DOA、GH-DOA和RK-DOA表现出强大的抗氧化作用，在第1天分别显著降低37%、54%和60%的活性氧水平，在第5天分别显著降低68%、73%和75%。第4天，TR-DOA、GH-DOA和RK-DOA分别显著降低了36%、51%和39%的脂褐素水平(P <；第8天，脂褐素水平显著降低34% (P <；0.01), 32% (P <；0.05), 33% (P <；分别为0.05)。同样，TR-DOA、GH-DOA和RK-DOA分别使内质网应激标志物HSP-4::GFP水平降低36%、37%和35% (P <；0.000 1)，分别增加38%、40%和45% (P <；1)第8天。与对照组相比，RK-DOA、GH-DOA和TR-DOA显著上调HSP-6::GFP (P <；0.0001, P <；0.001, P <；0.05)，耐热性增强(P <；0.0001, P <；0.001, P <；分别为0.01)。RK-DOA、GH-DOA和TR-DOA在第1天显著降低α-突触核蛋白聚集，分别降低31%、25%和18% (P <；0.0001, P <；0.0001, P <；0.01)，第3天分别增加32%、27%和12% (P <；0.01, P <；0.01, P >；分别为0.05)。RK-DOA、GH-DOA和TR-DOA延迟性麻痹(P <；0.0001，所有病例)，蠕虫活性显著增加(P <；0.001, P <；0.001, P <；分别为0.01)。RK-DOA、GH-DOA和TR-DOA也显著提高了第5天的体弯曲率(P <；0.001, P <；0.01, P <；0.0001)和第10天(P <；0.001, P <；0.0001, P <；0.01)。我们的研究结果表明，DOA，特别是RK-DOA，在秀丽隐杆线虫中具有很好的抗氧化和抗衰老作用。不同DOA变体之间的差异反应突出了培养环境在形成天然化合物生物活性方面的重要性。
{"title":"Alkaloids extracted from Dendrobium officinale grown in diverse environments exhibited robust antioxidative and antiaging activity","authors":"Yichen Liu , Arome Solomon Odiba , Qi Yu , Jin Xie , Nkwachukwu Oziamara Okoro , Guiyan Liao , Xinchen Zhu , Bin He , Cheng Jin , Wenxia Fang , Bin Wang","doi":"10.1016/j.jfutfo.2024.11.007","DOIUrl":"10.1016/j.jfutfo.2024.11.007","url":null,"abstract":"<div><div>Due to the increase in aging populations and the prevalence of aging-associated diseases, there is a growing interest in finding therapeutic interventions. Antioxidants play a vital role in mitigating the adverse conditions associated with aging. In this study, we investigated the impact of <em>Dendrobium officinale</em> alkaloids (DOA) cultivated in diverse environments on antioxidative and anti-aging responses in <em>Caenorhabditis elegans</em>. Three distinct sources of DOA, represented as tree (TR)-DOA, greenhouse (GH)-DOA, and rock (RK)-DOA, were examined. Following initial testing of three DOA concentrations, 10 µg/mL was selected, which increased the TJ1060 lifespan by 23%, and exhibited no toxicity. TR-DOA, GH-DOA, and RK-DOA exhibited robust antioxidative effects, significantly reducing reactive oxygen species levels by 37%, 54%, and 60%, respectively on day 1, and 68%, 73%, and 75% on day 5, respectively. On day 4, TR-DOA, GH-DOA, and RK-DOA significantly reduced lipofuscin levels by 36%, 51%, and 39%, respectively (<em>P</em> < 0.000 1). On day 8, lipofuscin levels were significantly reduced by 34% (<em>P</em> < 0.01), 32% (<em>P</em> < 0.05), and 33% (<em>P</em> < 0.05), respectively. TR-DOA, GH-DOA, and RK-DOA, likewise, reduced the level of the endoplasmic reticulum stress marker, HSP-4::GFP, by 36%, 37%, and 35%, respectively (<em>P</em> < 0.000 1) on day 1, and by 38%, 40%, and 45%, respectively (<em>P</em> < 0.000 1) on day 8. Compared to the control, RK-DOA, GH-DOA, and TR-DOA significantly upregulated HSP-6::GFP (<em>P</em> < 0.0001, <em>P</em> < 0.001, and <em>P</em> < 0.05, respectively), and enhanced heat stress resistance (<em>P</em> < 0.0001, <em>P</em> < 0.001, and <em>P</em> < 0.01, respectively). RK-DOA, GH-DOA, and TR-DOA significantly reduced <em>α</em>-synuclein aggregation by 31%, 25%, and 18% respectively on day 1 (<em>P</em> < 0.0001, <em>P</em> < 0.0001, and <em>P</em> < 0.01, respectively), and by 32%, 27%, and 12% on day 3 (<em>P</em> < 0.01, <em>P</em> < 0.01, and <em>P</em> > 0.05, respectively). RK-DOA, GH-DOA, and TR-DOA delayed paralysis (<em>P</em> < 0.0001, in all cases) and significantly increased worm activity (<em>P</em> < 0.001, <em>P</em> < 0.001, and <em>P</em> < 0.01, respectively). RK-DOA, GH-DOA, and TR-DOA also significantly increased the rate of body bend on day 5 (<em>P</em> < 0.001, <em>P</em> < 0.01, <em>P</em> < 0.0001) and day 10 (<em>P</em> < 0.001, <em>P</em> < 0.0001, <em>P</em> < 0.01). Our findings suggest that DOA, particularly RK-DOA, offers promising antioxidative and anti-aging benefits in <em>C. elegans</em>. The differential responses among DOA variants highlight the importance of the cultivation environment in shaping the bioactivity of natural compounds.</div></div>","PeriodicalId":100784,"journal":{"name":"Journal of Future Foods","volume":"5 6","pages":"Pages 591-604"},"PeriodicalIF":5.2,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143171715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
• Soy protein isolate softened jumbo squid gel texture, reduced gel hardness and enhanced strain value. • The mixed protein gels became loose and porous as the ratio of JSP/SPI decreased from 40:3 to 10:1, • The mixed protein gel when JSP:SPI=10:1 meets IDDSI level 5. As the global aging trend intensifies and the prevalence of dysphagia among the elderly rises, the need to develop food products suitable for elderly individuals with dysphagia becomes increasingly imperative. Therefore, this study established a dysphagia food gel system based on squid protein by adjusting the ratios between jumbo squid ( Dosidicus Gigas) protein (JSP) and soy protein isolate (SPI). As the JSP:SPI ratio decreased, the hardness and chewiness of the samples significantly decreased ( p < 0.05). These were consistent with the observed decrease in the storage modulus (G'), the increase in the strain value and the α-helix structure content. When the JSP:SPI ratio was 10:1, the hardness, chewiness, G' reached their minimum values, and the microstructure showed a loose and porous structure. Meanwhile, the strain and the content of disulfide bonds values peaked. Concurrently, the mixed protein gel achieved the softest texture, corresponding to level 5 according to the International Dysphagia Diet Standardisation Initiative. In conclusion, the addition of SPI softened the mixed protein gel, meeting international dysphagia standards and offering a novel approach for the development of dysphagia-friendly foods.
{"title":"Dysphagia-friendly gel from jumbo squid (Dosidicus Gigas) and soy protein isolate: synergistic softening effect, swallowing characteristics, textural and rheological properties","authors":"Lingyu Kong, Tingting Hao, Tingyu Feng, Ying Ren, Shu Hu, Xinghe Li, Songgang Xia, Zhaojie Li, Changhu Xue, Xiaoming Jiang","doi":"10.1016/j.jfutfo.2025.09.037","DOIUrl":"https://doi.org/10.1016/j.jfutfo.2025.09.037","url":null,"abstract":"• Soy protein isolate softened jumbo squid gel texture, reduced gel hardness and enhanced strain value. • The mixed protein gels became loose and porous as the ratio of JSP/SPI decreased from 40:3 to 10:1, • The mixed protein gel when JSP:SPI=10:1 meets IDDSI level 5. As the global aging trend intensifies and the prevalence of dysphagia among the elderly rises, the need to develop food products suitable for elderly individuals with dysphagia becomes increasingly imperative. Therefore, this study established a dysphagia food gel system based on squid protein by adjusting the ratios between jumbo squid ( Dosidicus Gigas) protein (JSP) and soy protein isolate (SPI). As the JSP:SPI ratio decreased, the hardness and chewiness of the samples significantly decreased ( p < 0.05). These were consistent with the observed decrease in the storage modulus (G'), the increase in the strain value and the α-helix structure content. When the JSP:SPI ratio was 10:1, the hardness, chewiness, G' reached their minimum values, and the microstructure showed a loose and porous structure. Meanwhile, the strain and the content of disulfide bonds values peaked. Concurrently, the mixed protein gel achieved the softest texture, corresponding to level 5 according to the International Dysphagia Diet Standardisation Initiative. In conclusion, the addition of SPI softened the mixed protein gel, meeting international dysphagia standards and offering a novel approach for the development of dysphagia-friendly foods.","PeriodicalId":100784,"journal":{"name":"Journal of Future Foods","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147332430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-11-01Epub Date: 2025-01-29DOI: 10.1016/j.jfutfo.2024.11.006
Keke Suo , Chaoqiang Zheng , Zhipeng Li , Limin Hao , Jiaqing Zhu , Changcheng Zhao , Yanling Shi , Juanjuan Yi , Jike Lu
Ionizing radiation (IR) can produce superfluous reactive oxygen species (ROS) and induce oxidative damage to human health. In view of the shortcomings of chemosynthetic radioprotectors, natural radioprotectors have garnered attention due to their effectiveness, safety, and suitability for long-term use. Natural active substances, such as polysaccharides, polyphenols and alkaloids, have been proved exert good radioprotective effect. In the present study, the main components and monosaccharide compositions of the polysaccharides from Poria cocos peels (PCPP) and its radioprotective activities against 60Co-γ induced oxidative damage in vitro and in vivo were evaluated. The results showed that PCPP contained (63.13 ± 3.19)% of total sugar and was composed of mannose, arabinose, glucuronic acid, galacturonic acid, glucose, galactose and fucose in a molar radio of 104.84:1.34:2.83:1.00:290.48:243.30:79.63. Moreover, PCPP exhibited significant antioxidant activity and could significantly reduce the damage of AML-12 cells under IR. Animal experiment results showed that PCPP could effectively reduce IR-induced oxidative damage of spleens and livers in mice, and alleviate the damage to the hematopoietic system. Furthermore, PCPP could greatly increase the activity of superoxide dismutase (SOD) and the content of glutathione (GSH) in serum, livers and spleens of 60Co-γ induced mice, and correspondingly reduce the accumulation of malondialdehyde (MDA), along with the prominent reduction of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in serum and livers in mice. Above results comprehensively indicated that PCPP exerted significant antioxidant activity and could effectively reduce the 60Co-γ induced damage in vitro and in vivo.
{"title":"Radioprotective effects of polysaccharides from Poria cocos peels against 60Co-γ induced oxidative damage in vitro and in vivo","authors":"Keke Suo , Chaoqiang Zheng , Zhipeng Li , Limin Hao , Jiaqing Zhu , Changcheng Zhao , Yanling Shi , Juanjuan Yi , Jike Lu","doi":"10.1016/j.jfutfo.2024.11.006","DOIUrl":"10.1016/j.jfutfo.2024.11.006","url":null,"abstract":"<div><div>Ionizing radiation (IR) can produce superfluous reactive oxygen species (ROS) and induce oxidative damage to human health. In view of the shortcomings of chemosynthetic radioprotectors, natural radioprotectors have garnered attention due to their effectiveness, safety, and suitability for long-term use. Natural active substances, such as polysaccharides, polyphenols and alkaloids, have been proved exert good radioprotective effect. In the present study, the main components and monosaccharide compositions of the polysaccharides from <em>Poria cocos</em> peels (PCPP) and its radioprotective activities against <sup>60</sup>Co-γ induced oxidative damage <em>in vitro</em> and <em>in vivo</em> were evaluated. The results showed that PCPP contained (63.13 ± 3.19)% of total sugar and was composed of mannose, arabinose, glucuronic acid, galacturonic acid, glucose, galactose and fucose in a molar radio of 104.84:1.34:2.83:1.00:290.48:243.30:79.63. Moreover, PCPP exhibited significant antioxidant activity and could significantly reduce the damage of AML-12 cells under IR. Animal experiment results showed that PCPP could effectively reduce IR-induced oxidative damage of spleens and livers in mice, and alleviate the damage to the hematopoietic system. Furthermore, PCPP could greatly increase the activity of superoxide dismutase (SOD) and the content of glutathione (GSH) in serum, livers and spleens of <sup>60</sup>Co-γ induced mice, and correspondingly reduce the accumulation of malondialdehyde (MDA), along with the prominent reduction of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in serum and livers in mice. Above results comprehensively indicated that PCPP exerted significant antioxidant activity and could effectively reduce the <sup>60</sup>Co-γ induced damage <em>in vitro</em> and <em>in vivo</em>.</div></div>","PeriodicalId":100784,"journal":{"name":"Journal of Future Foods","volume":"5 6","pages":"Pages 582-590"},"PeriodicalIF":5.2,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143104271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
• L. rhamnosus S2-1 with better hypoglycaemic and hypolipidemic properties than other strains was obtained from healthy infant faeces. • L. rhamnosus S2-1 increased expression levels of PI3K and AKT genes to alleviatet insulin resistance on HepG2 cells. • L. rhamnosus S2-1 raised expression levels of PPAR α and CPT1A genes to attenuate abnormal lipid metabolism on HepG2 cells. • L. rhamnosus S2-1 simultaneously ameliorated oxidative stress of abnormal glucose-lipid metabolism on HepG2. Disorders of glucose metabolism promote abnormalities in lipid metabolism, while abnormalities in lipid metabolism exacerbate imbalances in glucose metabolism. Together, these contribute to the development of metabolic syndrome, type 2 diabetes mellitus (T2DM), obesity, and cardiovascular disease. In this study, we isolated Lactobacillus rhamnosus S2-1 from fecal samples of healthy infants, based on its potential hypoglycemic and lipid-lowering properties. The underlying mechanisms through which this strain improves glucose and lipid metabolic disorders were further investigated using both in vitro cell models and in vivo animal experiments. The results demonstrated that L. rhamnosu S2-1 could improve body, liver and epididymal fat weight, liver/epididymal fat index, blood glucose and insulin, liver lipid accumulation and antioxidant parameters high-fat-fructose diet combined with a streptozotocin-induced (HFFD)-fed mice. It also modulated hepatic mRNA expression associated with glucose and lipid metabolism disorders. Meanwhile, in HepG2 cells from the insulin-resistant/high-fat model, the cell-free supernatant (CFS) and cell-free extracts (CFE) of S2-1 yielded similar results of animal studies. In both insulin-resistant/high-fat cell models and T2DM mice models, S2-1 effectively alleviated oxidative stress by suppressing free radicals and enhancing antioxidant enzyme activity, thereby mitigating glucose-lipid metabolic disorders. Notably, the CFS demonstrated superior efficacy overall compared to the CFE ( P < 0.05). This finding provides a theoretical basis and a therapeutic strategy to improve the vicious cycle process of glucose-lipid metabolism disorder interactions and to intervene in diabetes mellitus and hyperlipidaemia.
{"title":"Research on the regulatory mechanisms of Lactobacillus rhamnosus S2-1 on cellular lucose and lipid metabolism","authors":"Haoran Song, Wenjun Liu, Junrui Wu, Zhi Zhong, Yumeng Liu, Huijuan Li, Xing Wei, Shuang Zhang, Rina Wu, Yongfu Chen","doi":"10.1016/j.jfutfo.2025.09.039","DOIUrl":"https://doi.org/10.1016/j.jfutfo.2025.09.039","url":null,"abstract":"• L. rhamnosus S2-1 with better hypoglycaemic and hypolipidemic properties than other strains was obtained from healthy infant faeces. • L. rhamnosus S2-1 increased expression levels of PI3K and AKT genes to alleviatet insulin resistance on HepG2 cells. • L. rhamnosus S2-1 raised expression levels of PPAR α and CPT1A genes to attenuate abnormal lipid metabolism on HepG2 cells. • L. rhamnosus S2-1 simultaneously ameliorated oxidative stress of abnormal glucose-lipid metabolism on HepG2. Disorders of glucose metabolism promote abnormalities in lipid metabolism, while abnormalities in lipid metabolism exacerbate imbalances in glucose metabolism. Together, these contribute to the development of metabolic syndrome, type 2 diabetes mellitus (T2DM), obesity, and cardiovascular disease. In this study, we isolated Lactobacillus rhamnosus S2-1 from fecal samples of healthy infants, based on its potential hypoglycemic and lipid-lowering properties. The underlying mechanisms through which this strain improves glucose and lipid metabolic disorders were further investigated using both in vitro cell models and in vivo animal experiments. The results demonstrated that L. rhamnosu S2-1 could improve body, liver and epididymal fat weight, liver/epididymal fat index, blood glucose and insulin, liver lipid accumulation and antioxidant parameters high-fat-fructose diet combined with a streptozotocin-induced (HFFD)-fed mice. It also modulated hepatic mRNA expression associated with glucose and lipid metabolism disorders. Meanwhile, in HepG2 cells from the insulin-resistant/high-fat model, the cell-free supernatant (CFS) and cell-free extracts (CFE) of S2-1 yielded similar results of animal studies. In both insulin-resistant/high-fat cell models and T2DM mice models, S2-1 effectively alleviated oxidative stress by suppressing free radicals and enhancing antioxidant enzyme activity, thereby mitigating glucose-lipid metabolic disorders. Notably, the CFS demonstrated superior efficacy overall compared to the CFE ( P < 0.05). This finding provides a theoretical basis and a therapeutic strategy to improve the vicious cycle process of glucose-lipid metabolism disorder interactions and to intervene in diabetes mellitus and hyperlipidaemia.","PeriodicalId":100784,"journal":{"name":"Journal of Future Foods","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147331412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-11-01Epub Date: 2025-01-29DOI: 10.1016/j.jfutfo.2024.11.004
Ying Xu , Yueyan Zhang , Shiqing Song , Lingyun Yao , Min Sun , Huatian Wang , Chuang Yu , Qian Liu , Jun Lu , Tao Feng
Irradiation as a cold sterilization technology is increasingly used in chicken breast. The odor of animal food produced by medium dose irradiation is rarely reported. The purpose of this study was to investigate the effect of irradiation intensity on the distribution of volatile compounds in chicken breast, and to provide the appropriate irradiation methods for reference to decontamination of chicken breast in practice. The volatiles in chicken breast at different irradiation doses were investigated by headspace solid-phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS) and GC-ion mobility spectrometry (GC-IMS). There were 91 aroma compounds identified in this study, including 12 esters, 13 ketones, 15 aldehydes, 14 alcohols and so on. The volatiles in the irradiated chicken breasts were different from those in the non-irradiated chicken breasts. Sensory evaluation was conducted on irradiated chicken breasts, and partial least squares regression (PLSR) analysis was performed in combination with the volatile compound concentration to identify the characteristic compounds that contributed significantly to the fruity, musty, greasy, chicken flavor and paint flavor. It was indicated that (E)-2-octenal (odor activity value: 0.13−2.13), 1-octen-3-ol (7.66−113.44), nonanal (3.03−215.35), and acetoin (0.85−1.43) might be the characteristic compounds for irradiate-generated off-flavors. The irradiation intensity of 4 and 6 kGy had little negative effect on the flavor of chicken breast. In practical application, the irradiation dose of 4−6 kGy can be selected. Our study provided a reference for the selection of irradiation intensity in the processing of chicken breast, and revealed that the volatile substances had a great influence on the flavor of irradiated chicken breast, which laid a foundation for subsequent flavor regulation.
{"title":"Identification of flavor generated from irradiation of chicken breast via SPME-GC-MS and GC-IMS","authors":"Ying Xu , Yueyan Zhang , Shiqing Song , Lingyun Yao , Min Sun , Huatian Wang , Chuang Yu , Qian Liu , Jun Lu , Tao Feng","doi":"10.1016/j.jfutfo.2024.11.004","DOIUrl":"10.1016/j.jfutfo.2024.11.004","url":null,"abstract":"<div><div>Irradiation as a cold sterilization technology is increasingly used in chicken breast. The odor of animal food produced by medium dose irradiation is rarely reported. The purpose of this study was to investigate the effect of irradiation intensity on the distribution of volatile compounds in chicken breast, and to provide the appropriate irradiation methods for reference to decontamination of chicken breast in practice. The volatiles in chicken breast at different irradiation doses were investigated by headspace solid-phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS) and GC-ion mobility spectrometry (GC-IMS). There were 91 aroma compounds identified in this study, including 12 esters, 13 ketones, 15 aldehydes, 14 alcohols and so on. The volatiles in the irradiated chicken breasts were different from those in the non-irradiated chicken breasts. Sensory evaluation was conducted on irradiated chicken breasts, and partial least squares regression (PLSR) analysis was performed in combination with the volatile compound concentration to identify the characteristic compounds that contributed significantly to the fruity, musty, greasy, chicken flavor and paint flavor. It was indicated that (<em>E</em>)-2-octenal (odor activity value: 0.13−2.13), 1-octen-3-ol (7.66−113.44), nonanal (3.03−215.35), and acetoin (0.85−1.43) might be the characteristic compounds for irradiate-generated off-flavors. The irradiation intensity of 4 and 6 kGy had little negative effect on the flavor of chicken breast. In practical application, the irradiation dose of 4−6 kGy can be selected. Our study provided a reference for the selection of irradiation intensity in the processing of chicken breast, and revealed that the volatile substances had a great influence on the flavor of irradiated chicken breast, which laid a foundation for subsequent flavor regulation.</div></div>","PeriodicalId":100784,"journal":{"name":"Journal of Future Foods","volume":"5 6","pages":"Pages 561-571"},"PeriodicalIF":5.2,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143171714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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