Advanced Healthcare Materials最新文献

Multifunctional Hydrogel for Wound Healing

The cover illustrates a multifunctional La,N-CD-loaded hydrogel dressing for an infected wound. Blue-emissive carbon dots within the network disrupt bacterial membranes, while the hydrogel absorbs exudate and maintains moisture, supporting tissue repair. The artwork highlights antibacterial activity, moisture retention, mechanical robustness, and biocompatibility. More details can be found in the Research Article by Chun Che Lin and co-workers (DOI: 10.1002/adhm.71608).

Antioxidant MOF nanozyme-engineered PDLSCs

In their Research Article (DOI: 10.1002/adhm.71523), Daiyong Chao, Gang Ding, and co-workers develop an antioxidant nanozyme-engineered periodontal ligament stem cells (PDLSCs) strategy for diabetic wound healing. CuNi-MOF nanozymes protect PDLSCs from oxidative stress and senescence via JNK/ERK axis. The engineered cells accelerate re-pithelialization, angiogenesis, collagen deposition, and M2 macrophage polarization, thus promoting the healing of diabetes skin wounds.

The tumor microenvironment (TME) is a complex and heterogeneous milieu encompassing biological and biochemical events and diverse physical, chemical, and mechanical interactions between tumor and stromal cells. This heterogeneity plays a pivotal role in regulating tumor progression, metastasis, and resistance to therapy. In breast cancer, cancer-associated fibroblasts, adipocytes, endothelial cells, and immune cells all contribute to this intricate environment, necessitating precise spatial and cellular modeling to better replicate physiological conditions. Moreover, angiogenesis and metastasis are key contributors to breast cancer mortality. Therefore, three-dimensional (3D) models that incorporate co-cultures of cancer and stromal cells are essential for recreating realistic TMEs. This review highlights the advances in 3D bioprinting technologies for modeling the breast cancer TME. We discuss their potential in drug screening, explore their advantages and limitations, and outline future directions to enhance their fidelity and translational relevance.

Meniscal injury is a leading cause of early-onset osteoarthritis, yet regenerative options remain limited. This study investigates a nonwoven polyethylene terephthalate (PET) scaffold for meniscus tissue engineering and assesses its capacity to support mesenchymal stromal cell (MSC) proliferation and chondrogenic differentiation under dynamic loading. Human MSCs are seeded onto PET scaffolds (400–420 g/m², 85% porosity) and cultured for up to 21 days under basal medium (Ctr), chondrogenic differentiation conditions (ChD), or ChD combined with dynamic loading (ChD + Dyn, 12% strain, 1 Hz, 1 h/day, 5 days/week). PET scaffolds support uniform MSC adhesion and colonization. Compared with ChD alone, ChD+Dyn significantly increases cell proliferation and transiently upregulated chondrogenic markers (SOX9, ACAN, COL1A1, COL2A1) while suppressing the hypertrophic marker COL10A1. Although collagen deposition and construct biomechanics remained unchanged over 21 days, glycosaminoglycan accumulation was reduced in the ChD + Dyn group compared with ChD. RNA sequencing revealed distinct mechanosensitive transcriptional signatures induced by dynamic loading, particularly in genes associated with extracellular matrix remodeling, mechanotransduction, and developmental signaling pathways. These findings demonstrate that nonwoven PET provides a mechanically robust scaffold for meniscus tissue engineering and that dynamic loading promotes MSC proliferation while transiently regulating chondrogenic differentiation and mechanoadaptive matrix remodeling toward a meniscus-like phenotype.

As in vitro tissue models, including organoids, become thicker and more complex, diffusion-limited three-dimensional (3D) culture increasingly fails to sustain long-term viability, nutrient supply, and spatial microenvironmental regulation. Existing vascularization, perfusion, and chip-based strategies still struggle to integrate complex 3D architecture, durable perfusion, mechanical integrity, and controlled trans-wall exchange in thick constructs. Here, a fibril-reinforced, 3D-printed solid-walled zein vascular interface is developed to couple long-term perfusion stability with wall-mediated mass transport. Homologous zein fibrils enable direct printing of branched perfusable conduits with tunable porous walls, avoiding sacrificial hollow-channel templating while improving deformation resistance and preserving permeability during 30 days of continuous perfusion. Wall architecture establishes two application-specific transport regimes. In a regulated-exchange regime, the interface supports nutrient delivery in 33 × 21 × 11 mm cell-laden constructs, maintaining long-term survival and enhancing CD31 expression within a continuously perfused peri-conduit microenvironment. In a high-permeability regime, enlarged porous walls generate spatial transport gradients, distance-dependent heterogeneity, and enhanced permeability and retention (EPR)-relevant trans-wall nanocarrier transport with peri-conduit accumulation, enabling evaluation of photoresponsive liposomal delivery and polyaniline-mediated photothermal responses. This zein platform integrates printable biomaterial architecture, perfusion durability, and tunable wall-mediated transport for thick tissue support and EPR-relevant therapeutic evaluation in advanced in vitro model systems.

Wearable Bioelectronics for Smart Healthcare

In their Research Article (DOI: 10.1002/adhm.71548), Jiangtao Sun, Lijun Xu, Chengfeng Pan, and co-workers report a large language model (LLM)-driven throat-wearable sensing system (TWSS) for real-time laryngeal monitoring and swallowing assessment. TWSS integrates a flexible dual-sensitive sensor with wireless electronics and an S3-LLM framework for structured signal encoding and few-shot learning. In 20 participants, TWSS achieved 92.4% activity recognition and 87.6% swallowing assessment accuracy, demonstrating potential for continuous monitoring of swallowing disorders.

There is a critical need for novel therapeutic strategies to tackle multidrug-resistant bacterial infections. Artificial sweeteners (AS) specifically acesulfame potassium, sodium saccharin, and sodium cyclamate, have recently demonstrated antimicrobial activity against multidrug-resistant bacteria. In this study, polyvinyl alcohol (PVA)-borate hydrogel is developed as a carrier for antimicrobial AS to combat wound infections. Through extensive optimization, we developed cytocompatible 8% AS-loaded hydrogels with 3% PVA + 3% borax that reduced the viability of multidrug-resistant Acinetobacter baumannii AB5075 by 99.9% colony-forming unit enumeration following 1 h hydrogel treatment. Most currently available wound dressings have limited efficacy against bacterial biofilms, but we demonstrate that each of these sweeteners can attenuate A. baumannii and Pseudomonas aeruginosa dual-microbial biofilms. Haemolysis and cell viability assays demonstrate excellent blood compatibility and non-cytotoxic behavior of the sweetener-loaded hydrogels. To further evaluate the clinical potential of these AS-loaded hydrogels, we conducted a pilot Phase I clinical study with human volunteers focused on evaluating short-term safety and irritancy. This study revealed that the dressings had no adverse effects on the volunteers. This research underscores the translational potential of hydrogels augmented with AS and their capacity to overcome many of the hurdles that typically lead to wound dressing failure.