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Interaction of Bacteriocin-Capped Silver Nanoparticles with Food Pathogens and Their Antibacterial Effect 细菌素包覆银纳米颗粒与食品病原体的相互作用及其抑菌效果
Pub Date : 2012-06-15 DOI: 10.1080/19430892.2012.678757
T. Sharma, M. Sapra, Aradhana Chopra, Rekha Sharma, Supriya Deepak Patil, R. Malik, R. Pathania, N. Navani
ABSTRACT With the emergence of multiple-drug-resistant pathogens, the antibacterial property of silver in colloidal form has emerged as a potential candidate for combating infectious diseases. A combination of antibacterial agents along with nanosilver could prove to be more potent due to broadened antibacterial spectrum with possibly lower doses. In the present study, a facile single-step green method of synthesizing silver nanoparticles functionalized with an antibacterial peptide from a food-grade lactic acid bacterium is reported. The synthesized enterocin-coated silver nanoparticles showed broad-spectrum inhibition against a battery of food-borne pathogenic bacteria without any detectable toxicity to red blood cells. The present results evince that a new category of biocide based on silver nanoparticles coated with food-grade antibacterials can be developed using simple methods.
随着多重耐药病原体的出现,胶体银的抗菌特性已成为对抗传染病的潜在候选物。抗菌剂与纳米银的组合可能被证明更有效,因为抗菌谱更宽,剂量可能更低。在本研究中,报告了一种简单的单步绿色方法,合成了具有食品级乳酸菌抗菌肽功能化的纳米银。合成的肠球蛋白包覆银纳米颗粒对一系列食源性致病菌具有广谱抑制作用,对红细胞没有任何可检测到的毒性。目前的研究结果表明,可以用简单的方法开发出一种新型的基于银纳米粒子包被食品级抗菌药物的杀菌剂。
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引用次数: 38
Humidity Sensing Investigations on Nanostructured Zinc Stannate Synthesized via Chemical Precipitation Method 化学沉淀法合成纳米锡酸锌的湿度传感研究
Pub Date : 2012-03-20 DOI: 10.1080/19430892.2012.654742
B. Yadav, Rama Singh, Satyendra Singh, P. Dwivedi
ABSTRACT In this paper, nanoparticles of zinc stannate in different compositions were synthesized via chemical precipitation method. The as-synthesized material was characterized using differential scanning calorimetry. For the formation of crystalline zinc stannate, the synthesized material was annealed at 600°C. Surface morphologies of the samples were analyzed using scanning electron microscopy. The XRD pattern indicates the formation of nanocrystalline zinc stannate and it has a perovskite phase with an orthorhombic structure having a minimum crystallite size 4 nm. Pellets of the sensing materials were made by hydraulic press machine under a pressure of 616 MPa. Furthermore, the humidity sensing investigations of these sensing pellets were completed. Our results illustrate that zinc stannate in 1:4 weight ratio was most sensitive to humidity in comparison to other compositions under same conditions. Maximum sensitivity of the sensor was 3 GΩ/%RH. The results were reproducible up to 72% after 2 mon of ...
摘要:本文采用化学沉淀法合成了不同组成的锡酸锌纳米颗粒。用差示扫描量热法对合成材料进行了表征。为了得到结晶锡酸锌,将合成的材料在600℃下退火。用扫描电子显微镜分析了样品的表面形貌。XRD谱图表明,锡酸锌形成了纳米晶,具有正交结构的钙钛矿相,最小晶粒尺寸为4 nm。在616 MPa的压力下,采用液压机对传感材料进行制粒。此外,还完成了这些传感球团的湿度传感研究。结果表明,在同等条件下,锡酸锌在重量比为1:4时对湿度最敏感。传感器的最大灵敏度为3 GΩ/%RH。2个月后,结果的重现率高达72%。
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引用次数: 7
Nanotech Reinforcement onto Cellulosic Fibers: Green Remediation of Toxic Metals 纤维素纤维的纳米强化:有毒金属的绿色修复
Pub Date : 2012-03-20 DOI: 10.1080/19430892.2012.654744
S. Srivastava, Abhishek Kardam, K. R. Raj
ABSTRACT Strengthening the functional (COO−) group and nanotech reinforcement for binding of cationic metal species has been applied to cellulosic fibers. Modified Nano cellulosic fibers (NCFs) have been used for the remediation of cationic toxic metals from water bodies. Different polymerized NCFs exhibited enhancement in sorption efficiency (3 to 10%) and stability in terms of increased (3 to 5) regeneration cycles for Cd (II), Pb (II), Ni (II), and Cr (III) ions in single and multi-metal solutions. Novel NCFs have been characterized on the basis of Optical, TEM, FTIR, and TGA techniques and found to have enough potential for environmental clean-up.
增强官能团(COO−)和纳米技术增强阳离子金属物种的结合已被应用于纤维素纤维。改性纳米纤维素纤维(nfc)已被用于水体中阳离子有毒金属的修复。不同聚合nfc对Cd (II)、Pb (II)、Ni (II)和Cr (III)离子在单金属和多金属溶液中的再生周期增加3 ~ 5次,其吸附效率(3 ~ 10%)和稳定性均有所提高。基于光学、TEM、FTIR和TGA技术对新型nfc进行了表征,发现其具有足够的环境净化潜力。
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引用次数: 60
Green Synthesis and Antimicrobial Potential of Silver Nanoparticles 纳米银的绿色合成及其抗菌潜力
Pub Date : 2012-03-20 DOI: 10.1080/19430892.2012.656040
T. Sharma, Aradhana Chopra, M. Sapra, D. Kumawat, Supriya Deepak Patil, R. Pathania, N. Navani
ABSTRACT Developments in nanotechnology are leading to acceptance of this technology in day-to-day life as it continues to provide solutions and alternatives to technological, environmental, and health challenges. Central to all nanotechnology based applications are nanostructures, among which nanoparticles (NPs) are the ones most widely used. With a huge demand for various NPs, it is imperative that environmentally benign synthesis methods are developed. The majority of the existing procedures used for nanoparticle synthesis rely upon physical and chemical methods that sometimes utilize toxic and hazardous chemicals. The integration of the principles of green chemistry to nanotechnology toward the synthesis of “green” nanoparticles is a current requirement. One of the bioactive roles being investigated for NPs is their potential as antimicrobials. This review focuses on biomolecules, microbes, and phytoproducts in mediated green approaches for the synthesis of green silver nanoparticles (GSNPs) with due ...
纳米技术的发展正在导致人们在日常生活中接受这项技术,因为它继续为技术、环境和健康挑战提供解决方案和替代方案。所有基于纳米技术的应用的核心是纳米结构,其中纳米粒子(NPs)是应用最广泛的纳米结构。随着对各种NPs的巨大需求,开发对环境无害的合成方法势在必行。目前用于纳米颗粒合成的大多数程序依赖于物理和化学方法,有时使用有毒和危险的化学品。将绿色化学原理与纳米技术相结合,合成“绿色”纳米粒子是当前的需求。正在研究的NPs的生物活性作用之一是它们作为抗菌剂的潜力。本文综述了生物分子、微生物和植物产物介导的绿色方法合成绿色纳米银(GSNPs)的研究进展。
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引用次数: 18
Silver Nanoparticles Shoot Up from the Root of Daucus carrota (L.) 纳米银粒子从胡萝卜根部萌发
Pub Date : 2012-03-20 DOI: 10.1080/19430892.2012.654745
K. Mukunthan, S. Balaji
Extensive work has been done to explore the potential promises of plant systems in the synthesis of metal nanoparticles. Herein, a convenient and rapid process for the formation of silver nanoparticles with natural reducing and capping agents of Daucus carrota (L.) is reported. The synchronization of secondary metabolites and enzymes as reducing/capping agents plays a vital role in the synthesis and stability of silver nanoparticles for approximately two months. Stable silver nanoparticles were formed by reacting tap root extract of D. carrota with an aqueous solution of AgNO3, which reduced Ag++ to Ag0. The synthesized silver nanoparticles were further characterized using UV spectrophotometry, Energy Dispersive X-ray Spectroscopy (EDAX), Scanning Electron Microscopy (SEM), and X-ray Diffraction (XRD) methods. The synthesized silver nanoparticles were spherical and stable in aqueous medium ranges from 31–52 nm.
为了探索植物系统在金属纳米颗粒合成中的潜在前景,已经做了大量的工作。本文报道了一种利用天然胡萝卜还原封盖剂快速制备纳米银的方法。次级代谢物和酶作为还原/封盖剂的同步作用在大约两个月的银纳米颗粒的合成和稳定性中起着至关重要的作用。通过将胡萝卜根茎提取物与AgNO3水溶液反应,将ag++还原为Ag0,制备出稳定的纳米银颗粒。利用紫外分光光度法、能量色散x射线能谱法(EDAX)、扫描电镜(SEM)和x射线衍射(XRD)等方法对合成的纳米银进行了进一步的表征。合成的银纳米粒子呈球形,在31 ~ 52 nm的水介质中稳定存在。
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引用次数: 15
Synthesis and Characterization Techniques of Nanomaterials 纳米材料的合成与表征技术
Pub Date : 2012-03-20 DOI: 10.1080/19430892.2012.654738
R. Srivastava
ABSTRACT Earlier studies have shown that nanomaterials are the most promising materials for modern development of science and technology. In this brief review, attention will be focused on a detailed introduction, the synthesis methods to prepare ceramic nanoparticles, and various characterization techniques.
早期的研究表明,纳米材料是现代科学技术发展中最有前途的材料。本文将详细介绍纳米陶瓷的制备方法,以及各种表征技术。
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引用次数: 36
Consolidation of Sand Particles by Nanoparticles of Calcite after Concentrating Ureolytic Bacteria In Situ 溶尿细菌原位富集后方解石纳米颗粒对砂粒的固结作用
Pub Date : 2012-03-20 DOI: 10.1080/19430892.2012.654741
S. Al-Thawadi, R. Cord-Ruwisch, M. Bououdina
ABSTRACT Ureolytic bacteria in the presence of high concentration of urea and calcium ions will precipitate calcite crystals in the form of nanocrystal aggregates. Those aggregates consolidate sandy materials by forming bridging points between sand particles. Bacterial cells that were isolated in a previous study, Bacillus sp. MCP11 (DSM 23526), were successfully concentrated in situ by up-loading 3-void volumes of cells followed by cementation solution (calcium/urea). Sandstone with UCS of 1200 kPa was formed with three applications of cells and cementation solution without clogging the void spaces resulting in a permeable consolidated sand core. ESEM images, EDS, and XRD analysis for the consolidated sandstone showed nanoparticles of rhombohedral calcite crystals aggregates of very fine particles at the nanoscale forming point-to-point contact between the sand particles.
摘要:溶尿菌在高浓度尿素和钙离子存在下,会析出方解石晶体,以纳米晶聚集体的形式析出。这些集料通过在沙粒之间形成桥接点来巩固沙质物质。在先前的研究中分离的细菌细胞芽孢杆菌sp. MCP11 (DSM 23526),通过上载3空隙体积的细胞,然后用胶结液(钙/尿素)成功地原位浓缩。砂岩的UCS为1200 kPa,通过三次细胞和胶结液的应用形成,而不会堵塞空隙,从而形成渗透性固结砂芯。固结砂岩的ESEM图像、EDS和XRD分析显示,方解石晶体的纳米颗粒在纳米尺度上形成了非常细的颗粒聚集体,在砂粒之间形成了点对点接触。
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引用次数: 22
Biocompatible Hydrophilic CdSe Quantum Dots: Single-Step Synthesis 生物相容性亲水性CdSe量子点:单步合成
Pub Date : 2012-03-20 DOI: 10.1080/19430892.2012.654748
P. Khanna, K. Dhanabalan, P. More, S. Viswanathan, V. Renugopalakrishnan
ABSTRACT We herein present a simple one-step synthesis of CdSe quantum dots in aqueous medium using Na2SeSO3 as a source of Se and PVP and sodium succinate as the surfactant. The isolated orange to dark-red particles are re-dispersible in alcoholic and aq. medium. The optical spectra indicated blue shift of >150 nm. The hydrophilic quantum dots show excellent FWHM of approximately 44–60 nm in their photoluminescence spectra and the emission bands are centered around 570–590 nm. Absorption spectra maxima are observed in the range of 530–550 nm. The particle size from absorption wavelength is calculated to be about 5 nm and the XRD indicate cubic CdSe with impurities of selenium. It is observed that Se impurities enhanced PL intensity. Facile synthesis of CdSe QD has increasing value in biomedical applications.
摘要本文提出了一种在水介质中以Na2SeSO3为Se和PVP源,琥珀酸钠为表面活性剂,一步合成CdSe量子点的方法。分离的橙色至暗红色颗粒在酒精和水溶液中可再分散。光谱显示蓝移> 150nm。亲水性量子点的光致发光峰宽为44 ~ 60 nm,发射带集中在570 ~ 590 nm附近。吸收光谱最大值在530 ~ 550 nm范围内。从吸收波长计算得到的CdSe的粒径约为5 nm, XRD表明其为含硒的立方CdSe。观察到Se杂质增强了发光强度。快速合成CdSe量子点在生物医学上的应用价值越来越大。
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引用次数: 2
Fabrication and Calibration of an Ag Nanoparticles Sensor for Detecting the Melamine and NaCl Levels in Raw Milk 用于检测原料奶中三聚氰胺和氯化钠含量的银纳米颗粒传感器的制备和校准
Pub Date : 2012-02-07 DOI: 10.1080/19430892.2012.739475
R. Bagheri, A. Kompany, N. Shahtahmasbi
ABSTRACT A colorimetric, label-free, and aggregation-based Ag nanoparticles (AgNPs) probe was developed for the detection of melamine and NaCl. A color change from yellow to blue with increasing melamine concentration was observed. The plasmon absorbance of the formed AgNPs allowed quantitative detection of melamine. A sensitive nonlinear correlation existed between the absorbance and the logarithm of melamine concentration, ranging from 1000 to 0.25 ppm. Detection of NaCl at a concentration of 10 ppm in raw milk was also observed.
建立了一种比色、无标记、基于聚合的银纳米颗粒(AgNPs)探针,用于检测三聚氰胺和NaCl。随着三聚氰胺浓度的增加,颜色由黄色变为蓝色。形成的AgNPs的等离子体吸光度允许对三聚氰胺进行定量检测。吸光度与三聚氰胺浓度的对数之间存在敏感的非线性相关关系,范围为1000 ~ 0.25 ppm。还观察了原料奶中NaCl浓度为10ppm时的检测结果。
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引用次数: 0
The Effect of 4-Nitroaniline Concentration on Ag Nanoparticles for Detecting the Melamine Level in Milk 4-硝基苯胺浓度对银纳米粒子检测牛奶中三聚氰胺的影响
Pub Date : 2012-02-07 DOI: 10.1080/19430892.2012.738579
R. Bagheri, A. Kompany, N. Shahtahmasbi
ABSTRACT Silver nanoparticles were prepared by reduction of AgNO3 in dilute aqueous solutions of 4-nitroaniline. The effect of 4-nitroaniline concentration on Ag nanoparticles was investigated, and the sensitivity and selectivity of the prepared Ag nanoparticles solution on the presence of melamine in milk were studied. A color change from yellow to blue was observed with increasing melamine concentration. The characterization methods used were transmission electron microscopy (TEM) and ultraviolet-visible (UV-Vis) spectroscopy. The TEM images indicated that the silver nanoparticles solution consisted of well-dispersed agglomerates of spherical-shaped Ag nanoparticles with an average size of about 32 nm. The plasmon absorption peak of the silver nanoparticles was found at 397 nm. In addition, it was revealed that a sensitive nonlinear correlation existed between the absorbance and the logarithm of melamine concentrations ranging from 50 to 10 ppm.
在4-硝基苯胺的稀水溶液中还原AgNO3制备纳米银。研究了4-硝基苯胺浓度对银纳米粒子的影响,并研究了制备的银纳米粒子溶液对牛奶中三聚氰胺的敏感性和选择性。随着三聚氰胺浓度的增加,颜色由黄色变为蓝色。表征方法为透射电子显微镜(TEM)和紫外可见光谱(UV-Vis)。TEM图像表明,银纳米粒子溶液由均匀分布的球形银纳米粒子团块组成,平均尺寸约为32 nm。在397 nm处发现了银纳米粒子的等离子体吸收峰。此外,在50 ~ 10 ppm范围内,三聚氰胺浓度的吸光度与对数之间存在敏感的非线性相关关系。
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引用次数: 1
期刊
International Journal of Green Nanotechnology
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