Nanomedicine : nanotechnology, biology, and medicine最新文献_第5页

Nanotechnology-based delivery strategies for drugs and vaccines targeting blood stage malaria: A systematic review 针对血期疟疾的基于纳米技术的药物和疫苗递送策略：系统综述。

IF 4.6 2区医学 Q2 MEDICINE, RESEARCH & EXPERIMENTAL

Nanomedicine : nanotechnology, biology, and medicine

Pub Date : 2025-11-01 Epub Date: 2025-10-13 DOI: 10.1016/j.nano.2025.102869

Yohana Amos , Clarence Rubaka , Elingarami Sauli , Hulda Swai , Anneth Tumbo

The blood stage of malaria, where Plasmodium parasites invade red blood cells, accounts for most clinical symptoms and severe complications. However, current drugs and vaccines remain limited by drug resistance, toxicity, poor stability, and reduced overall efficacy. This review aimed to synthesize evidence on nanotechnology-based delivery systems for improving targeting specificity, enhancing drug and antigen stability, and optimizing therapeutic outcomes. Forty (40) studies from 2005 to 2025 were systematically analyzed, focusing on lipid, polymeric, inorganic, and protein-based nanoparticles targeting the blood stage. Results showed that functionalized nanocarriers with ligands targeting infected red blood cells significantly enhanced drug efficacy and reduced systemic toxicity. In vaccine development, nanoparticles used as antigen carriers elicited strong immune responses, achieving up to 83.3 % survival in in vivo preclinical models. Despite these promising outcomes, challenges such as scalable production, clinical translation, and regulatory approval persist. Overall, the findings highlight nanomedicine's transformative potential for malaria treatment and prevention.

疟疾的血液阶段，疟原虫侵入红细胞，是大多数临床症状和严重并发症的原因。然而，目前的药物和疫苗仍然受到耐药性、毒性、稳定性差和总体疗效降低的限制。本文综述了基于纳米技术的递送系统在提高靶向特异性、增强药物和抗原稳定性以及优化治疗结果方面的研究进展。从2005年到2025年，我们系统地分析了40项研究，重点是针对血液阶段的脂质、聚合物、无机和蛋白质纳米颗粒。结果表明，功能化纳米载体与配体靶向感染红细胞显著提高药物疗效和降低全身毒性。在疫苗开发中，用作抗原载体的纳米颗粒引发了强烈的免疫反应，在体内临床前模型中实现了高达83.3 %的存活率。尽管取得了这些有希望的成果，但诸如可扩展生产、临床转化和监管批准等挑战仍然存在。总的来说，这些发现突出了纳米医学在疟疾治疗和预防方面的变革潜力。

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引用次数: 0

Chitosan-based drug delivery system targeting the myocardial microenvironment: Delivery strategy and mechanism exploration for precise treatment of myocardial injury 基于壳聚糖的靶向心肌微环境给药系统：精准治疗心肌损伤的给药策略及机制探索

IF 4.6 2区医学 Q2 MEDICINE, RESEARCH & EXPERIMENTAL

Nanomedicine : nanotechnology, biology, and medicine

Pub Date : 2025-11-01 Epub Date: 2025-10-21 DOI: 10.1016/j.nano.2025.102868

YuanYuan Zuo , Yanyan Zhu , Xuying Ding , Leiyi Wang , Jiaxin Zheng , Qucheng Huang , Hewei Xu , Chang Liu

Myocardial injury (MI) is the primary pathogenic process in many cardiovascular disorders. And inadequate medication targeting, unregulated release, and severe side effects are major issues in treatment. Recently, chitosan-based nano-delivery systems have gained popularity in the precision treatment of cardiovascular diseases due to their high biocompatibility, biodegradability, and ease of chemical modification. These devices can be functionally tuned to respond to the cardiac microenvironment, resulting in tailored drug enrichment and sustained release. In this paper, we provide a systematic assessment of the progress of chitosan and its derivatives in MI treatments, including material design approach, environmental response mechanism, drug loading and release management, and mode of action. It also examines the limitations of clinical translation and predicts future development directions. The study shows that chitosan-based nanosystems have significant potential in regulating inflammation and anti-oxidative stress, as well as promoting vascular neovascularization and myocardial repair.

心肌损伤是许多心血管疾病的主要致病过程。治疗中的主要问题是药物靶向不充分、药物释放不规范以及严重的副作用。近年来，基于壳聚糖的纳米递送系统因其高生物相容性、可生物降解性和易于化学修饰而在心血管疾病的精确治疗中得到了广泛的应用。这些装置可以在功能上调整以响应心脏微环境，从而产生量身定制的药物富集和持续释放。本文综述了壳聚糖及其衍生物在心肌梗死治疗中的研究进展，包括材料设计方法、环境反应机制、药物加载与释放管理、作用方式等。探讨了临床翻译的局限性，并预测了未来的发展方向。研究表明，壳聚糖纳米系统在调节炎症和抗氧化应激、促进血管新生和心肌修复方面具有重要的潜力。

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引用次数: 0

Impact of carbon nanotubes on pulmonary disorders attributed to occupational and environmental exposures 碳纳米管对职业和环境暴露引起的肺部疾病的影响。

IF 4.6 2区医学 Q2 MEDICINE, RESEARCH & EXPERIMENTAL

Nanomedicine : nanotechnology, biology, and medicine

Pub Date : 2025-11-01 Epub Date: 2025-10-24 DOI: 10.1016/j.nano.2025.102871

Afzaal Nadeem Mohammad , Yesenia Moreno , Garrett Grischo , Ying Liang , Stephanie Iusim , Sally Suliman , Ting Wang , Vladimir V. Kalinichenko , Kenneth S. Knox , Mrinalini Kala

With widespread use of carbon nanotubes (CNTs) in manufacturing, the public is increasingly exposed to these materials being released into the environment, with concerns of potential adverse effects on respiratory health. Studies have demonstrated that exposure to CNTs initiates inflammatory cascades and oxidative stress. CNT inhalation challenge in rodents often produces granulomatous inflammation and lung fibrosis. CNT exposure causes TH2 asthmatic inflammation in animal models. CNTs are implicated in disrupting the delicate balance of extracellular matrix homeostasis, contributing to fibrotic remodeling. Limited mechanistic studies exist but epidemiological data suggest a link between CNT exposure and the development of fibrotic and granulomatous lung diseases. In this review, we will discuss the impact of CNT exposure on the respiratory system and how CNT can be used in modeling lung disease.

随着碳纳米管（CNTs）在制造业中的广泛使用，公众越来越多地接触到这些被释放到环境中的材料，并担心对呼吸健康产生潜在的不利影响。研究表明，暴露于碳纳米管会引发炎症级联反应和氧化应激。啮齿动物CNT吸入刺激常产生肉芽肿性炎症和肺纤维化。在动物模型中，碳纳米管暴露会引起TH2哮喘性炎症。碳纳米管与破坏细胞外基质稳态的微妙平衡有关，有助于纤维化重塑。目前存在有限的机制研究，但流行病学数据表明碳纳米管暴露与纤维化和肉芽肿性肺部疾病的发展之间存在联系。在这篇综述中，我们将讨论碳纳米管暴露对呼吸系统的影响以及碳纳米管如何用于模拟肺部疾病。

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引用次数: 0

Dual metabolic targeting liposomes potentiate triple-negative breast cancer radiosensitivity via glucose and glutathione starvation 双代谢靶向脂质体通过葡萄糖和谷胱甘肽饥饿增强三阴性乳腺癌放射敏感性。

IF 4.6 2区医学 Q2 MEDICINE, RESEARCH & EXPERIMENTAL

Nanomedicine : nanotechnology, biology, and medicine

Pub Date : 2025-11-01 Epub Date: 2025-10-19 DOI: 10.1016/j.nano.2025.102870

Henan Zhang , Yanbin Liu , Xuezhi Gao , Guoli Ji , Yanzhen Zheng , Fei Luo , Xing Qi , Shasha Zhao , Shanghui Guan , Cong Wang , Ming Lu

Triple-negative breast cancer (TNBC) frequently develops resistance to radiotherapy, while its metabolic reliance on glucose and glutamine presents new therapeutic targets for radiotherapy sensitization. This study developed a targeted nanoliposome (G/B-Lip-R) co-delivering glucose oxidase (GOD) and buthionine sulfoximine (BSO) to enhance radiotherapy through dual metabolic intervention. GOD catalyzes glucose oxidation to generate hydrogen peroxide (H₂O₂) while depleting tumor energy supplies, whereas BSO inhibits glutathione (GSH) synthesis to disrupt redox homeostasis. Their synergistic action significantly elevates intracellular reactive oxygen species (ROS) levels, thereby potentiating radiosensitivity. Both in vitro and in vivo studies demonstrated that G/B-Lip-R effectively targets tumors and significantly improves radiotherapy outcomes. This work innovatively combines nanocarriers with dual metabolic pathway modulation, offering a novel strategy to overcome TNBC radioresistance with important clinical translation potential.

三阴性乳腺癌（TNBC）经常对放疗产生耐药性，而其对葡萄糖和谷氨酰胺的代谢依赖为放疗增敏提供了新的治疗靶点。本研究开发了一种靶向纳米脂质体（G/ b - lipr）共同递送葡萄糖氧化酶（GOD）和丁硫氨酸亚砜胺（BSO），通过双代谢干预增强放疗。GOD催化葡萄糖氧化生成过氧化氢（H2O2），同时消耗肿瘤能量供应，而BSO抑制谷胱甘肽（GSH）合成，破坏氧化还原稳态。它们的协同作用显著提高细胞内活性氧（ROS）水平，从而增强放射敏感性。体外和体内研究均表明G/B-Lip-R能有效靶向肿瘤，显著改善放疗效果。本研究创新性地将纳米载体与双代谢途径调制相结合，为克服TNBC放射耐药提供了一种具有重要临床转化潜力的新策略。

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引用次数: 0

4HPR nanoformulation regulates MAPKAPK3/3pK signaling to control Bax phosphorylation and mitochondrial translocation to execute apoptosis in neuroblastoma 4HPR纳米制剂调节MAPKAPK3/3pK信号，控制Bax磷酸化和线粒体易位，实现神经母细胞瘤细胞凋亡。

IF 4.6 2区医学 Q2 MEDICINE, RESEARCH & EXPERIMENTAL

Nanomedicine : nanotechnology, biology, and medicine

Pub Date : 2025-11-01 Epub Date: 2025-09-19 DOI: 10.1016/j.nano.2025.102858

Angela Sharma, Atul Dev, Subhasree Roy Choudhury, Surajit Karmakar

Neuroblastoma (NB) is a neuroendocrine tumor derived from neural crest progenitor cells, commonly arising along the sympathetic nervous system, especially in the adrenal medulla. Despite therapeutic advances, the prognosis for advanced-stage NB remains poor, necessitating improved treatment options. 4HPR has demonstrated cytotoxicity in various tumors, including NB, with low systemic toxicity; however, its clinical use is restricted by poor solubility and bioavailability. To address this, we developed a human serum albumin-based nanoformulation of 4HPR using a simple desolvation method. This formulation effectively induced apoptosis in NB cells, marked by increased ROS generation, elevated Bax/Bcl-2 ratio, and enhanced cell detachment. Notably, we identified for the first time that MAPKAPK3 downregulation leads to reduced Bax phosphorylation and increased mitochondrial translocation. Co-immunoprecipitation confirmed a direct MAPKAPK3–Bax interaction, indicating MAPKAPK3 regulates Bax via phosphorylation. Our nanoformulation modulates this cross-talk, demonstrating promising translational potential as a novel therapeutic strategy for neuroblastoma.

神经母细胞瘤（Neuroblastoma， NB）是一种源自神经嵴祖细胞的神经内分泌肿瘤，常见于交感神经系统，尤其是肾上腺髓质。尽管治疗进展，晚期NB的预后仍然很差，需要改进治疗方案。4HPR对包括NB在内的多种肿瘤均有细胞毒性，但具有较低的全身毒性；然而，其临床应用受到溶解度和生物利用度差的限制。为了解决这个问题，我们开发了一种基于人血清白蛋白的4HPR纳米制剂，使用简单的溶解方法。该制剂有效诱导NB细胞凋亡，表现为ROS生成增加，Bax/Bcl-2比值升高，细胞脱离增强。值得注意的是，我们首次发现MAPKAPK3下调导致Bax磷酸化减少和线粒体易位增加。共免疫沉淀证实了MAPKAPK3-Bax的直接相互作用，表明MAPKAPK3通过磷酸化调节Bax。我们的纳米制剂可以调节这种串扰，作为神经母细胞瘤的一种新的治疗策略，显示出有希望的转化潜力。

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引用次数: 0

Zebrafish as a model for assessing the nanotechnology-based approaches in cancer diagnosis, therapy and theranostic: A historical review, trends and challenges 斑马鱼作为评估基于纳米技术的癌症诊断、治疗和治疗方法的模型：历史回顾、趋势和挑战。

IF 4.6 2区医学 Q2 MEDICINE, RESEARCH & EXPERIMENTAL

Nanomedicine : nanotechnology, biology, and medicine

Pub Date : 2025-11-01 Epub Date: 2025-10-10 DOI: 10.1016/j.nano.2025.102867

Jaqueline Cardoso Jacintho , Ana Clara Nogueira Ferreira , Thiago Lopes Rocha

Nanotechnology-based approaches (NBA) can improve tumor diagnosis and treatment. Thus, zebrafish (Danio rerio) emerge as a model system to investigate antitumoral effects, biodistribution and mechanism of action of nanomaterials (NMs). The current study aimed to summarize and critically analyze the literature concerning the use of zebrafish as an in vivo model for assessing the NBA in diagnosis, therapy and theranostics of cancer. Revised data (n = 95) showed an increasing number of publications in recent years. The main study approach was therapeutic (83.16 %), while diagnosis and theranostics represented 9.47 % and 7.37 %, respectively. 95.8 % used the embryo-larval stage of zebrafish. The most studied NM was the nanoparticles (NPs). Breast cancer, liver and melanoma were the tumors most studied. Overall, NMs can reduce chemotherapeutic drug toxicity, inhibit tumor growth, metastasis and angiogenesis, also promote tumor imaging and tracking. Zebrafish is a suitable emerging model system in cancer nanomedicine research.

基于纳米技术的方法（NBA）可以改善肿瘤的诊断和治疗。因此，斑马鱼（Danio rerio）成为研究纳米材料（NMs）的抗肿瘤作用、生物分布和作用机制的模型系统。本研究旨在总结和批判性分析有关使用斑马鱼作为体内模型来评估NBA在癌症诊断、治疗和治疗中的作用的文献。订正数据（n = 95）显示近年来出版物的数量不断增加。研究方法以治疗为主（83.16% %），诊断和治疗分别占9.47 %和7.37 %。95.8% 采用斑马鱼胚胎幼虫期。研究最多的纳米是纳米颗粒（NPs）。乳腺癌、肝癌和黑色素瘤是研究最多的肿瘤。总体而言，NMs可以降低化疗药物毒性，抑制肿瘤生长、转移和血管生成，促进肿瘤成像和跟踪。斑马鱼是肿瘤纳米医学研究中一种合适的新兴模型系统。

{"title":"Zebrafish as a model for assessing the nanotechnology-based approaches in cancer diagnosis, therapy and theranostic: A historical review, trends and challenges","authors":"Jaqueline Cardoso Jacintho , Ana Clara Nogueira Ferreira , Thiago Lopes Rocha","doi":"10.1016/j.nano.2025.102867","DOIUrl":"10.1016/j.nano.2025.102867","url":null,"abstract":"<div><div>Nanotechnology-based approaches (NBA) can improve tumor diagnosis and treatment. Thus, zebrafish (<em>Danio rerio</em>) emerge as a model system to investigate antitumoral effects, biodistribution and mechanism of action of nanomaterials (NMs). The current study aimed to summarize and critically analyze the literature concerning the use of zebrafish as an <em>in vivo</em> model for assessing the NBA in diagnosis, therapy and theranostics of cancer. Revised data (n = 95) showed an increasing number of publications in recent years. The main study approach was therapeutic (83.16 %), while diagnosis and theranostics represented 9.47 % and 7.37 %, respectively. 95.8 % used the embryo-larval stage of zebrafish. The most studied NM was the nanoparticles (NPs). Breast cancer, liver and melanoma were the tumors most studied. Overall, NMs can reduce chemotherapeutic drug toxicity, inhibit tumor growth, metastasis and angiogenesis, also promote tumor imaging and tracking. Zebrafish is a suitable emerging model system in cancer nanomedicine research.</div></div>","PeriodicalId":19050,"journal":{"name":"Nanomedicine : nanotechnology, biology, and medicine","volume":"70 ","pages":"Article 102867"},"PeriodicalIF":4.6,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145275425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dual-targeting exosome vaccine confers efficient protection against CVB3-induced myocarditis 双靶向外泌体疫苗可有效预防cvb3诱导的心肌炎。

IF 4.6 2区医学 Q2 MEDICINE, RESEARCH & EXPERIMENTAL

Nanomedicine : nanotechnology, biology, and medicine

Pub Date : 2025-11-01 Epub Date: 2025-10-02 DOI: 10.1016/j.nano.2025.102865

Qinghui Cao , Yihui Ge , Tong Wang, Ke Lin, Sidong Xiong, Yan Yue

Coxsackievirus B3 (CVB3) is a common cause of viral myocarditis, necessitating the development of prophylactic vaccines.. AD-Exo, a dual-targeting exosome vaccine, was developed to induce immune responses via targeting draining lymph nodes (dLNs) and dendritic cells (DCs). By incorporating an albumin-binding domain (ABD) peptide and a DC-guiding peptide (DCpep) into our previous CVB3 exosome vaccine (Exo), the AD-Exo vaccine demonstrated superior immunogenicity. It efficiently bound to mouse serum albumin, amplified antigen enrichment in dLNs, and enhanced DC uptake and maturation. Subcutaneous immunization in mice elicited significantly higher CVB3-specific serum neutralizing IgG with greater affinity than Exo and single-targeting vaccines. It induced robust T cell proliferation and CTL responses, increasing IFN-γ-producing CD4⁺ and CD8⁺ T cells. Ultimately, the AD-Exo vaccine reduced cardiac viral load, minimized histopathological damage, and significantly improved survival in challenged mice. This strategy provided novel perspectives for the development of dual-targeting prophylactic vaccines against viral myocarditis.

柯萨奇病毒B3 （CVB3）是病毒性心肌炎的常见病因，需要开发预防性疫苗。AD-Exo是一种双靶向外泌体疫苗，通过靶向引流淋巴结（dln）和树突状细胞（dc）诱导免疫应答。通过将白蛋白结合域（ABD）肽和dc引导肽（DCpep）整合到我们之前的CVB3外泌体疫苗（Exo）中，AD-Exo疫苗显示出优越的免疫原性。它能有效地与小鼠血清白蛋白结合，增强dln中抗原的富集，并促进DC的摄取和成熟。小鼠皮下免疫诱导的cvb3特异性血清中和IgG明显高于Exo和单靶向疫苗。它诱导T细胞增殖和CTL反应，增加产生IFN-γ的CD4+和CD8+ T细胞。最终，AD-Exo疫苗降低了心脏病毒载量，减少了组织病理学损伤，并显著提高了小鼠的存活率。这一策略为病毒性心肌炎双靶向预防疫苗的开发提供了新的视角。

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引用次数: 0

Schisandrin-loaded β-cyclodextrin nanoparticles for atherosclerosis therapy 五味子载β-环糊精纳米颗粒用于动脉粥样硬化治疗。

IF 4.6 2区医学 Q2 MEDICINE, RESEARCH & EXPERIMENTAL

Nanomedicine : nanotechnology, biology, and medicine

Pub Date : 2025-11-01 Epub Date: 2025-10-10 DOI: 10.1016/j.nano.2025.102866

Qiuxia Huang, Xinyao Liu, Jinjin Yu, Xinya Zhang, Siqi Wang, Lili Zhou, Xiaofeng Niu, Weifeng Li

Nanoparticle delivery systems have been extensively investigated as novel therapeutic strategies to promote drug-resistant disease. These nanoparticle formulations demonstrated improved bioavailability and enhanced tissue targeting. Also, there is growing acceptance of the value of traditional Chinese medicine in fighting disease. In this study, combining the advantages of nanomedicine with the characteristics of the acidic inflammatory microenvironment of atherosclerosis, a nanoplasmonic platform encapsulating the unstable drug Sch was designed for the treatment of atherosclerotic lesions. pH-responsive nanocarriers, an acid-labile material of acetylated β-cyclodextrin (β-CD) (Ac-bCD) were synthesized by chemical modification of β-CD. The resulting nanoparticles loaded with Sch (Sch-NPs) were prepared using a solvent evaporation method. In ApoE^−/− mice fed a high-fat diet, Sch-NPs alleviated arterial damage, inhibited lipid metabolism disorders, reduced plaque area, and promoted plaque stability. In addition, Sch-NPs effectively reduced inflammatory infiltration and oxidative stress by modulating the MAPK pathway. Our findings demonstrate the promising applications of pH-responsive nanoparticles loaded with Sch for enhanced disease therapies such as atherosclerosis.

纳米颗粒递送系统作为促进耐药疾病的新治疗策略已被广泛研究。这些纳米颗粒制剂显示出更好的生物利用度和增强的组织靶向性。此外，越来越多的人接受了中医在对抗疾病方面的价值。本研究结合纳米医学的优势和动脉粥样硬化酸性炎症微环境的特点，设计了包封不稳定药物Sch的纳米等离子体平台，用于动脉粥样硬化病变的治疗。以乙酰化β-环糊精（β-CD）（Ac-bCD）为原料，对β-CD进行化学修饰，合成了ph响应型纳米载体。采用溶剂蒸发法制备了Sch- nps纳米粒子。在喂食高脂肪饮食的ApoE-/-小鼠中，Sch-NPs减轻动脉损伤，抑制脂质代谢紊乱，减少斑块面积，促进斑块稳定性。此外，Sch-NPs通过调节MAPK通路有效减少炎症浸润和氧化应激。我们的研究结果表明，负载Sch的ph响应纳米颗粒在增强疾病治疗（如动脉粥样硬化）方面具有很好的应用前景。

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引用次数: 0

3D correlative light and electron microscopy reveals the uptake and processing of inorganic-organic hybrid nanoparticles into cancer cells 三维相关光镜和电子显微镜揭示了无机-有机杂化纳米颗粒在癌细胞中的吸收和加工过程。

IF 4.6 2区医学 Q2 MEDICINE, RESEARCH & EXPERIMENTAL

Nanomedicine : nanotechnology, biology, and medicine

Pub Date : 2025-11-01 Epub Date: 2025-10-22 DOI: 10.1016/j.nano.2025.102872

Louisa Herbsleb , David Wild , Henriette Gröger , Tim Schubert , Anna Maria Steyer , Julian Hennies , Frauke Alves , Claus Feldmann , Andreas Walter

Inorganic–organic hybrid nanoparticles (IOH-NPs) are a promising drug delivery system in oncology due to their high drug-load capacity. In this study, we established a 3D correlative light and electron microscopy (CLEM) workflow that combines confocal fluorescence microscopy (FM) with focused ion beam scanning electron microscopy (FIBSEM) to unambiguously identify and visualize the (sub)cellular uptake and processing of reference fluorescently labeled and zirconium-based IOH-NPs in murine H8N8 breast cancer cells. The 3D-CLEM workflow was set up without the need to add external fiducial markers since image correlation was achieved using lipid droplets as intrinsic correlative landmarks. We observed that all H8N8 breast cancer cells had taken up IOH-NPs after 4 h, and most IOH-NPs were found in clusters within the H8N8 cells. IOH-NPs were internalized by endocytosis within 2 h with increasing cellular concentrations over time and accumulated in endolysosomal vesicles over 24 h, while the overall endolysosomal volume increased between 2 and 6 h after IOH-NP incubation and returned to its original value thereafter, remaining stable for up to 48 h. The 3D-CLEM workflow also revealed changes in the morphology and density of the IOH-NPs inside endolysosomal vesicles, suggesting the dissolution of IOH-NPs after 2 h. We also observed mitochondrial swelling in IOH-NP exposed cells, suggesting stress responses even without drug load. The 3D-CLEM workflow provides new insights into the cellular tracking and processing of IOH-NPs and supports the development of novel nanomedicine strategies.

无机-有机杂化纳米颗粒（IOH-NPs）因其高载药量而成为一种很有前途的肿瘤药物递送系统。在这项研究中，我们建立了三维相关光学和电子显微镜（CLEM）工作流程，结合共聚焦荧光显微镜（FM）和聚焦离子束扫描电子显微镜（FIBSEM），明确地识别和可视化小鼠H8N8乳腺癌细胞中参考荧光标记和锆基IOH-NPs的（亚）细胞摄取和加工。3D-CLEM工作流程的建立无需添加外部基准标记，因为使用脂滴作为内在相关标记实现了图像相关性。我们观察到，在4 h (h)后，所有H8N8乳腺癌细胞都吸收了IOH-NPs，并且大多数IOH-NPs在H8N8细胞内呈簇状分布。IOH-NP在2 h内通过内吞作用内化，随着时间的推移细胞浓度增加，并在24 h内积聚在内溶酶体囊泡中，而IOH-NP孵育后的2至6 h内溶酶体总体体积增加，此后恢复到初始值，保持稳定长达48 h。3D-CLEM工作流程还揭示了内溶酶体囊泡内IOH-NPs的形态和密度的变化，表明IOH-NPs在2 h后溶解。我们还观察到IOH-NP暴露细胞的线粒体肿胀，表明即使没有药物负荷也会出现应激反应。3D-CLEM工作流程为IOH-NPs的细胞跟踪和处理提供了新的见解，并支持了新型纳米医学策略的开发。

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引用次数: 0

Inhibition of growth of the human chronic leukemia cancer cell line K562 using capsaicin-containing nanofibers: an in vitro study 含辣椒素纳米纤维抑制人慢性白血病K562细胞生长的体外研究

IF 4.6 2区医学 Q2 MEDICINE, RESEARCH & EXPERIMENTAL

Nanomedicine : nanotechnology, biology, and medicine

Pub Date : 2025-11-01 Epub Date: 2025-10-03 DOI: 10.1016/j.nano.2025.102864

Mohadeseh Heidarzadeh , Habib Hamidinezhad , Fatemeh Hedayati Tabari , Mohammad Karimian

This study aims to investigate the anticancer properties of capsaicin, the active substance of red pepper, in different concentrations (2 %, 6 %, and 10 %) by chitosan and polyvinyl alcohol nanofiber substrate, against the K562 leukemia cell line. For this purpose, chitosan (Cs) and polyvinyl alcohol (PVA) polymers were used to produce nanofibers with a 20/80 ratio by electrospinning, with capsaicin serving as the anticancer drug. The properties of the fabricated nanofibers were evaluated by field emission scanning electron microscopy. Also, gold nanoparticles were used to analyze and compare its effectiveness against the K562 cancer cell line. This cell line was prepared from Pasteur cell bank and cultured in DMEM medium. Subsequently, the anticancer effect of synthetic nanofibers in different concentrations was assessed by performing survival test, apoptosis by annexin and propidium iodide staining, and cell scratch assay. The electron microscopy study demonstrated the uniformity and purity of the nanofiber structure. The results showed that capsaicin, dose-dependently, reduced the viability of K562 cells after 72 h (P < 0.01). The apoptotic assay also indicated that the induction of apoptosis significantly increased by PVA/Cs/Caps(2 %) and PVA/Cs/Au(5 %)/Caps(10 %) compounds in the studied cell line (P < 0.0001). Furthermore, scratch assay at 24, 48, and 72 h demonstrated that the mentioned compounds possess anti-migration potential, particularly at 48 h. Our results suggest that capsaicin in nanofiber substrate can show anticancer properties against the K562 leukemia cell line. Therefore, this compound can be considered a potential candidate for the treatment of leukemia.

以壳聚糖和聚乙乙醇纳米纤维为底物，研究不同浓度（2 %、6 %和10 %）的红辣椒活性物质辣椒素对K562白血病细胞的抗癌作用。为此，以壳聚糖（Cs）和聚乙烯醇（PVA）聚合物为原料，以辣椒素为抗癌药物，采用静电纺丝法制备了20/80比例的纳米纤维。利用场发射扫描电镜对制备的纳米纤维的性能进行了评价。此外，还利用金纳米颗粒对K562癌细胞的杀伤效果进行了分析和比较。该细胞系由巴斯德细胞库制备，在DMEM培养基中培养。随后，通过存活实验、膜联蛋白和碘化丙啶染色细胞凋亡实验和细胞划痕实验来评估不同浓度合成纳米纤维的抗癌作用。电镜研究表明，纳米纤维结构均匀，纯度高。结果表明，辣椒素在72 h (P

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引用次数: 0