Process Biochemistry最新文献_第2页

Protein hydrolysates from black soldier fly larvae (Hermetia illucens): Experimental design, ultrafiltration fractionation, and kinetics of bioactive peptide production 黑兵蝇幼虫（Hermetia illucens）蛋白质水解物：实验设计、超滤分离和生物活性肽生产动力学

IF 4 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Process Biochemistry

Pub Date : 2026-04-01 Epub Date: 2026-02-09 DOI: 10.1016/j.procbio.2026.02.005

Yuri Matheus Silva Amaral , Leonardo Buzaneli Cristianini, Ruann Janser Soares de Castro

The improvement of the biological properties of insect proteins has been widely studied with a view to their inclusion in foods for humans and animals. The aim of this work was to study the production of bioactive peptides through enzymatic hydrolysis of proteins from black soldier fly larvae. For this purpose, an experimental mixture design was employed using the proteases Flavourzyme, Alcalase and Neutrase as factors, and antioxidant activity of the protein hydrolysates as the response of interest. The results revealed that the combination of Flavourzyme (2/3), Alcalase (1/6) and Neutrase (1/6) resulted in protein hydrolysates with the highest values of antioxidant activity, quantified by the ABTS (588.04 µmol TE/g), DPPH (30.15 µmol TE/g) and FRAP (76.29 µmol TE/g) methods. The kinetic study demonstrated that the proteases in combination maintained 80 % of the enzymatic activity through 180 min of hydrolysis. Furthermore, the hydrolysates were ultrafiltered and the peptides between 5 and 3 kDa showed greater antioxidant activity. According to data obtained from liquid chromatography-mass spectrometry (LC-MS/MS) and in silico analysis, the peptides YPGEL, AIGGW, LAWQF, and LGPPGAG showed the most promising antioxidant activity. The results obtained demonstrated the potential of enzymatic hydrolysis to selectively modify the proteins of black soldier fly larvae.

改善昆虫蛋白的生物学特性已被广泛研究，以期将其纳入人类和动物的食品中。本研究的目的是研究通过酶解黑兵蝇幼虫蛋白质生产生物活性肽。为此，采用混合试验设计，以风味酶、碱性酶和中和酶为因子，以蛋白水解产物的抗氧化活性为目标反应。结果表明，Flavourzyme（2/3）、Alcalase（1/6）和Neutrase（1/6）组合产生的蛋白水解产物具有最高的抗氧化活性，通过ABTS（588.04µmol TE/g）、DPPH（30.15µmol TE/g）和FRAP（76.29µmol TE/g）方法测定。动力学研究表明，在180 min的水解过程中，蛋白酶组合保持了80 %的酶活性。对水解产物进行超滤，发现5 ~ 3 kDa之间的肽具有较强的抗氧化活性。液相色谱-质谱联用（LC-MS/MS）和硅质谱分析结果显示，YPGEL、AIGGW、LAWQF和LGPPGAG肽具有较好的抗氧化活性。结果表明，酶解对黑兵蝇幼虫蛋白有选择性修饰的潜力。

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引用次数: 0

Polyhydroxyalkanoate production: Surplus storage stimulating selective growth; feasible soap digestion for downstream recovery and characterization 聚羟基烷酸酯生产：剩余储存刺激选择性生长；可行的肥皂消化下游回收和表征

IF 4 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Process Biochemistry

Pub Date : 2026-04-01 Epub Date: 2026-02-05 DOI: 10.1016/j.procbio.2026.02.004

Sydney T-Thy Nguyen-Huynh , Li Wan Yoon , Adeline Seak May Chua , Yin Hui Chow

Process enhancement for microbial PHA production by mixed cultures is still limited in approaching the industrial scale, which expects better performance in terms of PHA volumetric productivity (Q_vol) and sustainable PHA recovery. This study investigated feasibility of pulse feeding (PF) mode for PHA accumulation, soap digestion and characterization. The store-grow relationship in mixed cultures highlighted importance of PHB surplus storage (supply-dependence but no competition with other metabolic activities) in stimulating the selective growth of PHB storing bacteria. PF mode (3233 mg glycerol/pulse) significantly improved Q_vol to 246 mg/(L.h) with lowered PHB content in biomass to 62 % due to effective mobilization of stored PHB. When reducing dosage per pulse by half, PHB content maximized at 98 % was compromised by a slight decrease in Q_vol. to 209 mg/(L.h). With approximately 90 % PHB yield and purity, the alternative soap digestion was highly comparable to the halogenated solvent extraction. However, PHB degradation constituted to 17 % PHB loss, low Mw at nearly 900 Da, T_m of 89–133℃ and crystallization of 50–123 %, affecting PHB quality. The PHB bioplastic product was characterized for their thermal and mechanical properties. This study demonstrated the feasible PHB process configuration, i.e., culture enrichment by coupled feeding, PHB accumulation under PF mode in the upstream followed by soap digestion in downstream recovery.

通过混合培养提高微生物PHA生产的工艺在接近工业规模方面仍然受到限制，这在PHA体积生产率（Qvol）和可持续PHA回收率方面期望有更好的表现。本研究探讨了脉冲加料（PF）模式在PHA积累、肥皂消化和表征方面的可行性。混合培养中的储存-生长关系突出了PHB剩余储存（依赖供应但不与其他代谢活动竞争）在刺激PHB储存细菌选择性生长方面的重要性。PF模式（3233 mg甘油/脉冲）显著提高Qvol至246 mg/(L.h)，由于有效动员储存的PHB，生物量中PHB含量降低至62 %。当每脉冲剂量减少一半时，PHB含量在98 %时达到最大值，Qvol略有下降。到209年 毫克/ (L.h)。替代皂溶法的PHB收率和纯度约为90 %，与卤化溶剂萃取法相当。PHB的降解造成PHB损失17 %，Mw低，接近900 Da， Tm 89 ~ 133℃，结晶50 ~ 123 %，影响了PHB的质量。对PHB生物塑料制品的热性能和力学性能进行了表征。本研究论证了可行的PHB工艺配置，即通过耦合投料进行培养富集，在PF模式下进行上游PHB积累，下游回收进行肥皂消化。

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引用次数: 0

Solid-state fermentation of Daldinia eschscholtzii ZZNS-Y2, an approach to produce jasminodiol by Gardenia jasminoides-derived fungus 栀子属真菌固态发酵制备栀子二醇的研究

IF 4 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Process Biochemistry

Pub Date : 2026-04-01 Epub Date: 2026-02-11 DOI: 10.1016/j.procbio.2026.02.015

Jian-Wei Dong , Xiao-Xin Xu , Xue-Jiao Li , Liang Deng , Xiao-Yan Wang , Meng-yao Lv

Jasminodiol, an iridoid (monoterpenoid) compound isolated from the fruit of Gardenia jasminoides, has been reported to exhibit potent antityrosinase and anxiolytic activities. Currently, it is obtained exclusively through extraction from plant sources, with no microbial fermentation route established to date. This study aims to produce iridoids via endophytic fungi isolated from G. jasminoides. As a result, jasminodiol was successfully obtained from the solid-state fermentation (SSF) products of Daldinia eschscholtzii ZZNS-Y2, an endophytic fungus derived from G. jasminoides. The chemical structure of jasminodiol was elucidated based on spectroscopic data from ¹H and ¹³C NMR analyses. Furthermore, key fermentation parameters including substrate type, temperature, and fermentation time, were investigated. The high yield of jasminodiol (6.72 ± 0.20 mg/g) was achieved when SSF was conducted using corn niblet as the substrate at 28 °C for 40 d. This is the first report on the production of jasminodiol using microorganisms rather than plant extraction.

Jasminodiol是一种从栀子果实中分离出来的环烯醚萜类化合物，具有有效的抗酪氨酸酶和抗焦虑活性。目前，它完全是通过从植物中提取而获得的，迄今为止还没有建立微生物发酵途径。本研究旨在利用栀子内生真菌生产环烯醚萜。结果表明，从栀子内生真菌Daldinia eschscholtzii ZZNS-Y2的固态发酵（SSF）产物中获得了栀子二醇。利用1H和13C核磁共振光谱对茉莉二醇的化学结构进行了分析。此外，还研究了发酵的关键参数，包括底物类型、温度和发酵时间。以玉米片为底物，在28℃条件下发酵40 d，茉莉二醇的产率最高（6.72 ± 0.20 mg/g）。这是利用微生物而非植物提取生产茉莉二醇的首次报道。

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引用次数: 0

Enhanced denitrification in microbial electrolysis cells by employing riboflavin and reduced graphene oxide modified cathodes 利用核黄素和还原氧化石墨烯修饰阴极增强微生物电解细胞的反硝化作用

IF 4 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Process Biochemistry

Pub Date : 2026-04-01 Epub Date: 2026-02-03 DOI: 10.1016/j.procbio.2026.02.001

Xiang Li , Tingting Zhao , Xiaomin Hu , Xinyue Wang , Zhengdong Liu , Haimeng Hou

Nitrate pollution poses a significant threat to aquatic ecosystems and public health due to its high solubility and mobility in groundwater. Microbial electrolysis cells (MECs) offer a promising approach for nitrate removal from water; however, their denitrification efficiency remains limited. A composite cathode consisting of reduced graphene oxide-modified carbon cloth further electrodeposited with riboflavin (RF/rGO/CC) was employed in MECs to enhance denitrification performance. Over a 10-day operational period, the residual nitrate concentration in the catholyte of the RF/rGO/CC group decreased to 34.48 % of the initial level, compared with 54.33 % in the unmodified carbon cloth group. Scanning electron microscopy and confocal laser scanning microscopy revealed significantly increased biofilm density and microbial viability on the modified cathode. Electrochemical analyses confirmed that the RF/rGO/CC electrode exhibited superior electrochemical activity, which was reflected by a higher nitrate reduction peak observed in CV and a lower charge transfer resistance revealed by EIS. Moreover, the modified cathode enriched electroactive denitrifying bacteria and significantly increased the abundances of key functional genes involved in the denitrification pathway, including napA, narG, nirK, nirS, and nosZ. This study presents a promising strategy for enhancing nitrate removal efficiency in bioelectrochemical systems through cathode surface modification.

硝酸盐污染由于其在地下水中的高溶解度和流动性，对水生生态系统和公众健康构成重大威胁。微生物电解池（MECs）是一种很有前途的去除水中硝酸盐的方法。然而，它们的脱氮效率仍然有限。将还原氧化石墨烯-核黄素修饰碳布复合阴极（RF/rGO/CC）用于mec中，以提高其脱氮性能。在10天的运行周期内，RF/rGO/CC组阴极电解液中的残留硝酸盐浓度降至初始水平的34.48 %，而未改性碳布组则为54.33 %。扫描电镜和共聚焦激光扫描显微镜显示，改性阴极上的生物膜密度和微生物活力显著增加。电化学分析证实RF/rGO/CC电极具有优异的电化学活性，这体现在CV中观察到较高的硝酸盐还原峰和EIS显示的较低的电荷转移电阻。此外，改性后的阴极富集了电活性反硝化细菌，并显著增加了反硝化途径中关键功能基因的丰度，包括napA、narG、nirK、nirS和nosZ。本研究提出了一种通过阴极表面修饰来提高生物电化学系统中硝酸盐去除效率的有前景的策略。

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引用次数: 0

Heterologous expression of phospholipase A1 in Streptomyces mobaraensis and its application in crude oil degumming 磷脂酶A1在莫巴拉链霉菌中的异源表达及其在原油脱胶中的应用

IF 4 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Process Biochemistry

Pub Date : 2026-04-01 Epub Date: 2026-02-12 DOI: 10.1016/j.procbio.2026.02.009

Hongjian Xue , Jingwen Zhou , Guocheng Du , Song Liu

Phospholipase A1 (PLA1) selectively hydrolyzes the sn-1 ester of phospholipids and is ideal for enzymatic oil degumming. To efficiently produce PLA1, this study heterologously expressed the saPLA1 gene from Streptomyces albidoflavus NA297 in the genome-reduced strain Streptomyces mobaraensis smYS1-Δtg, and analyzed the enzymatic properties and degumming performance of the recombinant enzyme. Results showed that under the control of the strong promoter Pgapdh and the transglutaminase (TGase) signal peptide, secretory activity peaked at 39.4 U/mL within 48 h. It exhibited optimal activity at 50°C and pH 6.0, with good stability over pH 5–8. Additionally, saPLA1 was significantly activated by Mn^2 + , Fe²⁺ and citrate, and functioned in a Ca²⁺-independent manner. Under optimized process conditions, saPLA1 reduced the phosphorus content in crude soybean oil from 113.01 to 7.27 mg/kg within 2.5 h, meeting the physical refining requirement. This study provides a novel strategy for the industrial production and application of food-grade saPLA1.

磷脂酶A1 （PLA1）选择性地水解磷脂的sn-1酯，是理想的酶促油脂脱胶。为了高效生产PLA1，本研究将产自白黄链霉菌NA297的saPLA1基因异源表达到基因组还原菌株mobaraensis smYS1-Δtg中，并对重组酶的酶学性质和脱胶性能进行了分析。结果表明，在强启动子Pgapdh和转谷氨酰胺酶（TGase）信号肽的控制下，48 h内的分泌活性最高达到39.4 U/mL。在50℃、pH 6.0条件下活性最佳，在pH 5 ~ 8范围内稳定性较好。此外，saPLA1被Mn2 +、Fe2+和柠檬酸盐显著激活，并以Ca2+独立的方式发挥作用。在优化后的工艺条件下，saPLA1在2.5 h内将粗豆油中的磷含量从113.01降低到7.27 mg/kg，满足物理精炼要求。本研究为食品级saPLA1的工业化生产和应用提供了新的策略。

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引用次数: 0

An enzymatic approach for producing chitin from snow crab and American lobster seashells 用酶法从雪蟹和美洲龙虾贝壳中制备几丁质

IF 4 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Process Biochemistry

Pub Date : 2026-03-01 Epub Date: 2025-12-25 DOI: 10.1016/j.procbio.2025.12.015

Pauline Potier, Gabriela Vollet Marson, Ariane Tremblay, Alain Doyen, Lucie Beaulieu

Shellfish processing plants generate large volumes of by-products rich in high-value biomolecules such as proteins, minerals, and chitin. Currently, chitin extraction is mainly carried out by chemical hydrolysis using strong acids and bases, a method with a large environmental impact. To address these concerns, a sustainable enzymatic approach was developed for extracting chitin from snow crab and American lobster shells. Different acid-active proteases were tested to identify the most effective enzyme for simultaneous deproteinization and demineralization of the shells. Pepsin demonstrated the highest efficiency, achieving over 95 % demineralization and 78 % deproteinization. The process was further optimized to minimize enzyme concentration and hydrolysis time. The respective optimum parameters for crab and lobster were 3130 and 2070 U of pepsin per gram of protein for 2.5 h. In these conditions, deproteinization rates of 74.0 % and 81.1 % and demineralization rates of 94.5 % and 90.8 % were achieved for crab and lobster shells, respectively. Additionally, soluble proteins from the enzymatic hydrolysis were recovered and characterized, demonstrating their potential for use in animal feed and for bioactive peptides production.

贝类加工厂产生大量富含高价值生物分子的副产品，如蛋白质、矿物质和几丁质。目前，甲壳素的提取主要是利用强酸强碱进行化学水解，这种方法对环境影响较大。为了解决这些问题，开发了一种可持续的酶法从雪蟹和美国龙虾壳中提取甲壳素的方法。测试了不同的酸活性蛋白酶，以确定同时进行壳脱蛋白和脱矿的最有效酶。胃蛋白酶表现出最高的效率，达到95% %以上的脱矿和78% %以上的脱蛋白。进一步优化工艺，使酶浓度和水解时间降至最低。在2.5 h条件下，螃蟹和龙虾的最佳酶解参数分别为3130和2070 U / g蛋白质。在此条件下，蟹壳和龙虾壳的脱蛋白率分别为74.0 %和81.1 %，脱矿率分别为94.5 %和90.8 %。此外，从酶解中回收并表征了可溶性蛋白，证明了它们在动物饲料和生物活性肽生产中的潜力。

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引用次数: 0

Revealing and manipulating the LmbU-mediated connection between lincomycin production and the ectABCD gene cluster in S. lincolnensis 揭示和操纵lmbu介导的林肯葡萄球菌林可霉素生产与ectABCD基因簇之间的联系

IF 4 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Process Biochemistry

Pub Date : 2026-03-01 Epub Date: 2026-01-07 DOI: 10.1016/j.procbio.2026.01.002

Yue Mao , Jiang Ye , Ruida Wang , Haizhen Wu , Huizhan Zhang

Lincomycin, an antibiotic produced by Streptomyces lincolnensis, is widely used to treat Gram-positive bacterial and anaerobic infections. This study found that LmbU, a positive regulatory factor within the lincomycin biosynthesis gene cluster, can positively regulate the extracellular target gene cluster ectABCD (ect), which is involved in the synthesis of ectoine/hydroxyectoine. By knocking out ect, the lincomycin production was increased 2.8-fold. The addition of exogenous L-Asp in both xbl1 and Δect strains confirmed that the increased lincomycin production in Δect was due to enhanced precursor supply. Furthermore, osmotic stress experiments demonstrated that moderate NaCl concentrations could increase both ect expression and lincomycin production, while higher osmotic pressures resulted in decreased production and biomass. Together, these findings provide new mechanistic insights and offer a theoretical basis for the rational design of high-production industrial strains.

林可霉素是一种由林肯链霉菌产生的抗生素，广泛用于治疗革兰氏阳性细菌和厌氧感染。本研究发现LmbU是林可霉素生物合成基因簇内的正调控因子，可以正调控参与外托因/羟外托因合成的细胞外靶基因簇ectABCD （ect）。通过敲除等，林可霉素的产量提高了2.8倍。在xbl1和Δect菌株中添加外源L-Asp证实Δect菌株林可霉素产量的增加是由于前体供应的增加。此外，渗透胁迫实验表明，中等NaCl浓度可以增加ect表达和林可霉素产量，而较高的渗透压会导致林可霉素产量和生物量下降。总之，这些发现提供了新的机理见解，并为合理设计高产工业菌株提供了理论基础。

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引用次数: 0

Ultrasonic-alkaline-thermal pretreatment on cassava sago waste for the energy efficient biomethane production 超声波-碱-热预处理木薯西米废渣的高效生物甲烷生产

IF 4 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Process Biochemistry

Pub Date : 2026-03-01 Epub Date: 2026-01-28 DOI: 10.1016/j.procbio.2026.01.018

Preethi, Harshitha Nainar, V. Karunya, G. Neha, R. Preetha, Rajeev Kumar Bhaskar, P. Rajaguru, J. Rajesh Banu

Globally, cassava tuber is considered a staple food in many developing countries, and it is processed to extract starch from the tubers. Industrial production of sago from cassava tuber generates a considerable quantity of waste with high organic content. However, its intricate structure limits hydrolysis, the first step during bioenergy production. In this study, ultrasonic pretreatment and combinations of ultrasonic-alkali or ultrasonic-alkali-thermal pretreatment were tried to improve hydrolysis. Ultrasonic pretreatment of sago industrial waste (SIW) resulted in 1.53 % solubilization at 140 W ultrasonic power. While the combination of alkali (pH 11) with ultrasonic power of 140 W resulted in a mild increment in solubilization to 2 %. The introduction of thermal treatment with ultrasonic alkali pretreatment caused a drastic improvement in COD solubilization to 29.6 % at 80°C. Improvement in COD solubilization subsequently resulted in greater biomethane generation (303.4 mL/g VS_consumed) for ultrasonic-alkaline-thermal pretreatment compared to others. Similarly, the energy analysis shows a positive net energy of 109.55 kWh and an energy ratio of 1.54. Hence, the proposed combination is scalable for industrial biomethane production.

在全球范围内，木薯块茎被认为是许多发展中国家的主食，木薯块茎经过加工，从块茎中提取淀粉。从木薯块茎中提取西米的工业生产产生了大量有机含量高的废物。然而，其复杂的结构限制了水解，这是生物能源生产的第一步。本研究尝试了超声波预处理以及超声-碱或超声-碱-热联合预处理来改善水解。在140 W的超声功率下，西米工业废渣（SIW）的增溶率为1.53 %。而碱（pH 11）与140 W的超声功率组合，可使增溶量轻度增加至2 %。在80℃时，超声波碱预处理的引入使COD的增溶率显著提高到29.6 %。与其他方法相比，超声-碱-热预处理的COD增溶作用的改善导致了更多的生物甲烷生成（303.4 mL/g vs消耗量）。同样，能量分析显示净能量为109.55 kWh，能量比为1.54。因此，提议的组合是可扩展的工业生物甲烷生产。

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引用次数: 0

Impact of variable aeration on partial nitritation/anammox (PN/A): Insights from batch testing and microbial analysis 可变曝气对部分硝化/厌氧氨氧化（PN/A）的影响：来自批量试验和微生物分析的见解

IF 4 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Process Biochemistry

Pub Date : 2026-03-01 Epub Date: 2026-01-14 DOI: 10.1016/j.procbio.2026.01.007

Weronika Borowska , Martyna Godzieba , Slawomir Ciesielski , Monika Zubrowska-Sudol

This study explores the influence of variable aeration strategies on microbial interactions within the partial nitritation/anammox (PN/A) process. To overcome the limitations of relying solely on microbial abundance, this research integrates high-throughput DNA sequencing, reverse transcription-quantitative polymerase chain reaction (RT-qPCR), and batch tests to link microbial function and adaptation to variable aeration strategies. Four operational stages were investigated: continuous aeration with a dissolved oxygen (DO) concentration of 1.5 mg O₂/L; intermittent aeration at a ratio of 1:2 with the same DO; increased DO to 2.5 mg O₂/L while maintaining the intermittent regime; and equal duration of aerated and non-aerated phases (1:1) with DO at 2.5 mg O₂/L. Among these, the third stage intermittent aeration at 2.5 mg O₂/L proved to be the most favorable for the PN/A process, yielding the highest anammox activity and effective suppression of nitrite-oxidizing bacteria. Results show that the rate of nitrogen conversion is related to the particular structure of microbial communities, as revealed by network analysis. Importantly, even low-abundance functional groups contributed significantly to nitrogen removal under favorable environmental conditions. The combined methodological approach allowed for an effective correlation between microbial structure and activity, offering a valuable framework for interpreting microbial responses to operational changes.

本研究探讨了不同曝气策略对部分硝化/厌氧氨氧化（PN/A）过程中微生物相互作用的影响。为了克服单纯依赖微生物丰度的局限性，本研究整合了高通量DNA测序、逆转录-定量聚合酶链反应（RT-qPCR）和批量测试，将微生物的功能和适应与不同的曝气策略联系起来。研究了四个操作阶段：溶解氧（DO）浓度为1.5 mg O₂/L的连续曝气；在相同DO条件下，以1:2的比例间歇曝气；将DO增加到2.5 mg O₂/L，同时保持间歇状态；在2.5 mg O₂/L的DO下，充气相和非充气相（1:1）的持续时间相等。其中，第三阶段间歇曝气2.5 mg O₂/L最有利于PN/A工艺，厌氧氨氧化活性最高，对亚硝酸盐氧化菌的抑制效果最好。结果表明，氮转化速率与微生物群落的特定结构有关。重要的是，在有利的环境条件下，即使是低丰度的官能团也能显著地促进氮的去除。综合方法学方法允许微生物结构和活动之间的有效关联，为解释微生物对操作变化的反应提供了有价值的框架。

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引用次数: 0

Mitigation of antibiotic-resistant bacteria isolated from hospital effluent by lytic bacteriophage infection 通过溶解噬菌体感染减少医院流出物中分离的耐药细菌

IF 4 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Process Biochemistry

Pub Date : 2026-03-01 Epub Date: 2026-01-09 DOI: 10.1016/j.procbio.2026.01.001

Hayeon Lee, Jeongdong Choi

Antibiotic-resistant bacteria (ARB) released from hospital wastewater pose a significant risk to public health and receiving water bodies. This study evaluated the applicability of phage-based biocontrol for selectively suppressing hospital-derived ARB. Raoultella spp. isolated from treated hospital wastewater exhibited multidrug resistance, including resistance to β-lactams, colistin, and vancomycin. A lytic bacteriophage (Raoul-phage) infecting the isolated ARB was characterized, and its stability was confirmed across a broad range of temperatures, pH levels, and toxic chemical exposures. Batch-reactor assays demonstrated that the abundance of Raoultella spp. was reduced from 75 % to 38 % within 24 h, indicating effective host-specific lytic activity. These results highlight the feasibility of employing lytic bacteriophages for targeted ARB mitigation in hospital wastewater and suggest potential applicability for integration or retrofitting within existing wastewater treatment systems.

医院废水释放的耐药细菌（ARB）对公共卫生和接收水体构成重大风险。本研究评估了基于噬菌体的生物防治在选择性抑制医院源性ARB中的适用性。从处理过的医院废水中分离的Raoultella spp表现出多药耐药，包括对β-内酰胺类、粘菌素和万古霉素的耐药。对感染分离的ARB的裂解噬菌体（Raoul-phage）进行了表征，并在广泛的温度、pH值和有毒化学物质暴露范围内证实了其稳定性。批反应器实验表明，Raoultella spp.丰度在24 h内从75 %降低到38 %，表明有效的宿主特异性裂解活性。这些结果强调了在医院废水中使用裂解噬菌体靶向缓解ARB的可行性，并表明在现有废水处理系统中集成或改造的潜在适用性。

引用次数: 0