Biosensors and Bioelectronics最新文献_第7页

Active-site rich nanostructure with dual-enhanced electron transfer for ultra-sensitive electrochemiluminescence detection of DNA methylation in colon cancer 具有双增强电子转移的富活性位点纳米结构用于超灵敏电化学发光检测结肠癌DNA甲基化。

IF 10.5 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2026-05-15 Epub Date: 2026-01-31 DOI: 10.1016/j.bios.2026.118470

Xiao Tian , Yuting Hou , Liang Luan , Lingzhi Ye , Junjie Yuan , Yating Zhang , Mingliang Li , Xiangmeng Qu , Nan Wan

Traditional DNA methylation assays for colon cancer still face challenges of insufficient sensitivity, limited specificity, and low cost-efficiency in clinical samples. Herein, we develop an electrochemiluminescence biosensor based on a dual-enhanced electron transfer mechanism using DNA tetrahedral nanostructure probes for the ultrasensitive detection of methylated DNA related to colon cancer. The DNA tetrahedral structure probe overcomes the shortcomings of poor adaptability caused by the low binding efficiency and high spatial steric hindrance in complex clinical samples. The electrochemiluminescence biosensor uses active-site-rich TiO₂-Ti₃C₂ MXenes@AgNPs hybrid nanocomposites as the electro-active substrate. These nanocomposites exhibited accelerated electron transfer and shortened the reaction paths due to the intrinsic photoelectric activity of TiO₂ and the surface plasmon resonance (SPR) effect between AgNPs and TiO₂. Furthermore, the three-dimensional (3D) self-assembled AuNPs-ABEI nanoluminous spheres substantially improved the luminescence intensity by concentrating more AuNPs to accelerate electron transfer while immobilizing additional ABEI molecules. The dual-enhanced electron transfer strategy significantly accelerated the kinetics of interfacial electron transfer. Metal nanoparticles (such as AgNP, AuNP, and TiO₂) generate SPR-induced local electric fields under electrochemical excitation. The electric fields enhance the electron transfer and amplify the electrochemiluminescence intensity, thereby achieving ultra-sensitive detection of the methylated Septin9 gene with a detection limit as low as 8.2 aM. Clinical sample validation using clinical serum samples from healthy controls (n = 100) and colon cancer patients (n = 100) has shown that the biosensor achieves comparable performance to the gold standard method in terms of both sensitivity and specificity, demonstrating equivalent detection efficacy to pyrosequencing.

传统的结肠癌DNA甲基化检测方法在临床样品中仍然面临灵敏度不足、特异性有限、成本效益低等挑战。在此，我们开发了一种基于双增强电子传递机制的电化学发光生物传感器，使用DNA四面体纳米结构探针进行与结肠癌相关的甲基化DNA的超灵敏检测。DNA四面体结构探针克服了在复杂临床样品中结合效率低、空间位阻高而导致适应性差的缺点。电化学发光生物传感器采用富活性位点TiO2-Ti3C2 MXenes@AgNPs杂化纳米复合材料作为电活性衬底。由于TiO2的固有光电活性和AgNPs与TiO2之间的表面等离子体共振（SPR）效应，这些纳米复合材料具有加速电子转移和缩短反应路径的特性。此外，三维（3D）自组装的AuNPs-ABEI纳米发光球通过聚集更多的AuNPs来加速电子转移，同时固定额外的ABEI分子，从而大大提高了发光强度。双增强电子转移策略显著加快了界面电子转移动力学。金属纳米粒子（如AgNP、AuNP和TiO2）在电化学激发下产生spr诱导的局部电场。电场增强了电子传递，放大了电化学发光强度，从而实现了对甲基化的Septin9基因的超灵敏检测，检测限低至8.2 aM。使用健康对照（n = 100）和结肠癌患者（n = 100）的临床血清样本进行的临床样品验证表明，该生物传感器在灵敏度和特异性方面的性能与金标准方法相当，具有与焦磷酸测序相当的检测效果。

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引用次数: 0

Ion-induced plasmonic microneedle patches for ultrasensitive SERS detection Hemoporfin in interstitial fluids 离子诱导等离子体微针贴片用于超灵敏SERS检测间质液中的血红蛋白

IF 10.5 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2026-05-05 DOI: 10.1016/j.bios.2026.118778

Dan Li , Shuai Zhao , Weida Zhang , Yongsheng Li , Tanemura Masaki , Gang Ma , Yusi Peng , Yong Yang

Currently, the “one-size-fits-all” therapeutic window strategy for Hemoporfin-mediated photodynamic therapy of port-wine stains overlooks intralesional heterogeneity and interindividual pharmacokinetic differences. Simultaneously, this strategy lacks real-time feedback and personalized adjustment mechanisms. Therefore, the real-time monitoring of hemoporfin distribution in blood vessel and interstitial fluid is very important for finding the best laser illumination time window depended on personalized characterizes including the height, weight and so on. This study developed an ultra-sensitive sensor by integrating surface-enhanced Raman spectroscopy technology with microneedles (SERS-MNs) for monitoring the distribution dynamics of hemoporfin in blood and interstitial fluid within animal skin. Specifically, calcium ions were employed as an aggregating agent to induce the aggregation of gold/silver nanocage particles on the microneedle surface, thereby forming abundant SERS-enhanced “hot spots”. Using this sensor, characteristic Raman fingerprint signals of hemoporfin were successfully obtained for the first time, with a detection limit as low as 50 pg/mL. Furthermore, the SERS-MNs sensor not only quantitatively detected hemoporfin in pig skin but also successfully captured characteristic hemoporfin Raman signals in mouse interstitial fluid, blood, and treated patient blood. This revealed the spatiotemporal distribution mechanism of hemoporfin within the vascular system and interstitial fluid. This advancement will aid in determining the optimal therapeutic window for patients, thereby preventing both overtreatment and undertreatment, ultimately achieve precise therapy characterized by “individualization, visualization, and dynamization”.

目前，“一刀切”的治疗窗口策略用于hemopor蛋白介导的葡萄酒斑光动力治疗，忽略了病灶内的异质性和个体间的药代动力学差异。同时，该策略缺乏实时反馈和个性化调整机制。因此，根据身高、体重等个性化特征，实时监测血管和组织间液中的毛细孔蛋白分布，对于寻找最佳的激光照射时间窗具有重要意义。本研究将表面增强拉曼光谱技术与微针（SERS-MNs）相结合，开发了一种超灵敏传感器，用于监测动物皮肤内血液和间质液中的血孔蛋白分布动态。具体而言，利用钙离子作为聚集剂，诱导金/银纳米笼粒子在微针表面聚集，形成丰富的sers增强“热点”。利用该传感器首次成功获得了血红蛋白的特征拉曼指纹信号，检出限低至50 pg/mL。此外，SERS-MNs传感器不仅可以定量检测猪皮中的气孔蛋白，还可以成功捕获小鼠间质液、血液和治疗后患者血液中的特征气孔蛋白拉曼信号。这揭示了血管系统和间质液中孔血蛋白的时空分布机制。这一进步将有助于确定患者的最佳治疗窗口，从而防止过度治疗和治疗不足，最终实现以“个体化、可视化和动态化”为特征的精确治疗。

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引用次数: 0

Single-atom co anchored UiO-66 to boost electrochemiluminescence of MoS2 for bioanalysis 单原子共锚定UiO-66增强MoS2电化学发光用于生物分析

IF 10.5 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2026-05-01 Epub Date: 2026-01-24 DOI: 10.1016/j.bios.2026.118432

Jinwen Zhao , Guomin Yang , Yifan Ruan , Ruo Yuan , Shihong Chen

Single-atom catalysts (SACs) hold significant promise for improving the electrochemiluminescence (ECL) efficiency. However, the preparation of SACs reported in the ECL field usually relies on carbon-nitrogen-based materials as substrates and requires high-temperature pyrolysis (>800 °C), suffering from the limitation in the anchoring materials and the deficiency of insufficient active site density caused by high-temperature calcination. This work developed zirconium-based metal-organic framework UiO-66 as the carrier to successively anchor the Co single-atom (Co_SA) and the ECL emitter MoS₂ under mild conditions (not exceeding 200 °C). Co_SA not only can optimize the electronic structure of MoS₂ to effectively promote the formation of Co(IV)=O, but also can act as the co-reactant accelerator to efficiently catalyze the reduction of coreactant S₂O₈²⁻. Meanwhile, the Mo-O-Zr bimetallic sites formed between UiO-66 and MoS₂ can enhance the charge carrier mobility within MoS₂. As a result, the ECL emission of MoS₂ at −1.6 V was remarkably boosted when using step pulse as the electrochemical method. The synthesized MoS₂@Co_SA/UiO-66 composite integrated an aptamer recognition-driven cascaded polymerase amplification to achieve ultrasensitive ECL detection of p-Tau as crucial biomarker for Alzheimer's disease (AD). Co_SA/UiO-66 exhibits broad application prospects in improving ECL efficiency. MoS₂@Co_SA/UiO-66 builds a highly sensitive ECL sensing platform for detecting the biomarkers of AD and other diseases.

单原子催化剂（SACs）在提高电化学发光效率方面具有重要的前景。然而，ECL领域报道的SACs的制备通常依赖于碳氮基材料作为底物，并且需要高温热解（>800 °C），这受到锚定材料的限制以及高温煅烧导致活性位点密度不足的缺点。本工作开发了锆基金属有机骨架uuo -66作为载体，在温和条件下（不超过200 °C）连续锚定Co单原子（CoSA）和ECL发射极MoS2。CoSA不仅可以优化MoS2的电子结构，有效促进Co(IV)=O的形成，还可以作为助反应物促进剂，有效催化助反应物S2O82−的还原。同时，在UiO-66与MoS2之间形成的Mo-O-Zr双金属位可以提高MoS2内部载流子的迁移率。结果表明，在−1.6 V条件下，采用步进脉冲的电化学方法可以显著提高MoS2的ECL发射量。合成的MoS2@CoSA/UiO-66复合物整合了适体识别驱动的级联聚合酶扩增，实现了p-Tau作为阿尔茨海默病（AD）关键生物标志物的超灵敏ECL检测。CoSA/UiO-66在提高ECL效率方面具有广阔的应用前景。MoS2@CoSA/UiO-66构建了一个高灵敏度的ECL传感平台，用于检测AD等疾病的生物标志物。

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引用次数: 0

Routine monitoring of microRNAs in salivary exosomes using a cellulose nanofiber sheet 使用纤维素纳米纤维片对唾液外泌体中的microrna进行常规监测

IF 10.5 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2026-05-01 Epub Date: 2026-01-22 DOI: 10.1016/j.bios.2026.118436

Taiga Ajiri , Min Zhang , Naoya Mizukami , Mikiko Iida , Shota Kawaguchi , Yurie Sekihara , Kunanon Chattrairat , Zetao Zhu , Yoshinobu Baba , Hirotaka Koga , Takao Yasui

Liquid biopsy is a promising approach for non-invasive disease monitoring, offering the potential for frequent sampling and early risk assessment. Among various biofluids, saliva is particularly well-suited for daily sampling due to its ease of collection and minimal invasiveness. Here, we present a cellulose nanofiber sheet as a novel platform for the rapid, low-volume recovery and stable storage of miRNAs associated with small extracellular vesicles in saliva. The sheet captures vesicles via its nanoscale porous networks and preserves their contents at room temperature, enabling efficient extraction of vesicle-associated miRNAs. Compared to ultracentrifugation, the method demonstrated superior miRNA recovery and required only 10 μL of saliva and less than 1 min of processing. Analysis of salivary miRNA profiles from healthy individuals and cancer patients revealed distinct expression patterns, enabling identification of candidate cancer-related miRNAs. Furthermore, daily saliva sampling during 20 days demonstrated the feasibility of longitudinal miRNA profiling and highlighted the effects of factors such as food intake. These findings underscore the potential of cellulose nanofiber sheets as a practical tool for salivary miRNA monitoring, with applications in personalized health management, early risk detection, and decentralized testing environments.

液体活检是一种很有前途的非侵入性疾病监测方法，提供了频繁采样和早期风险评估的潜力。在各种生物液体中，唾液因其易于收集和最小的侵入性而特别适合于日常采样。在这里，我们提出了一种纤维素纳米纤维片作为快速、小体积回收和稳定储存唾液中与细胞外小泡相关的mirna的新平台。该薄片通过其纳米级多孔网络捕获囊泡，并在室温下保存其内容物，从而能够有效提取囊泡相关的mirna。与超离心相比，该方法的miRNA回收率更高，仅需10 μL唾液，处理时间不到1 min。对健康个体和癌症患者唾液miRNA谱的分析揭示了不同的表达模式，从而鉴定出候选的癌症相关miRNA。此外，20天内每日唾液采样证明了纵向miRNA分析的可行性，并强调了食物摄入等因素的影响。这些发现强调了纤维素纳米纤维片作为唾液miRNA监测实用工具的潜力，可用于个性化健康管理、早期风险检测和分散测试环境。

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引用次数: 0

Roll-to-roll printed in-pad biosensors for colorimetric detection of key biomarkers in artificial vaginal fluid 用于人工阴道液中关键生物标志物比色检测的卷对卷印刷衬垫生物传感器

IF 10.5 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2026-05-01 Epub Date: 2026-01-26 DOI: 10.1016/j.bios.2026.118433

Ziheng Wang , Semin Kwon , Tianhao Yu , Yumin Dai , Young L. Kim , Chi Hwan Lee

Gynecological conditions often lack accessible, non-invasive diagnostic tools, leading to delayed detection and treatment, especially in resource-limited settings. Vaginal discharge and menstrual blood offer abundant but underutilized biomarkers for diagnosing gynecological disorders. Although sanitary pads represent an ideal platform for continuous fluid monitoring, direct high-throughput fabrication of sensors onto commercial pads remains challenging. Here, we introduce a roll-to-roll (R2R) wax-printing method optimized for large-scale production of fluidic colorimetric sensor patches designed for integration into sanitary pads. Utilizing specially formulated wax inks, this scalable approach enables consistent, cost-effective sensor fabrication. The sensor patches were functionalized with colorimetric reagents, incorporating enzymatic sensing elements for uric acid and glucose and physicochemical indicators for pH and moisture. This configuration enabled robust multi-analyte detection with clear and interpretable visual outputs, observable directly by the naked eye or via smartphone-based analysis. Validation in artificial vaginal fluid (AVF) confirmed reliable sensor performance, underscoring a practical and scalable platform with potential to advance proactive women's health monitoring in both home-based and point-of-care settings.

妇科疾病往往缺乏可获得的非侵入性诊断工具，导致检测和治疗延迟，特别是在资源有限的环境中。阴道分泌物和经血为诊断妇科疾病提供了丰富但未充分利用的生物标志物。尽管卫生巾是连续流体监测的理想平台，但在商业卫生巾上直接制造高通量传感器仍然具有挑战性。在这里，我们介绍了一种卷对卷（R2R）蜡印方法，该方法针对大规模生产设计用于集成到卫生巾中的流体比色传感器贴片进行了优化。利用特殊配方的蜡油墨，这种可扩展的方法可以实现一致的，具有成本效益的传感器制造。用比色试剂对传感器贴片进行功能化，包括尿酸和葡萄糖的酶敏元件以及pH和水分的理化指标。这种配置实现了强大的多分析物检测，具有清晰和可解释的视觉输出，可直接通过肉眼或基于智能手机的分析观察到。在人工阴道液（AVF）中的验证证实了传感器的可靠性能，强调了一个实用且可扩展的平台，具有在家庭和护理点环境中推进主动妇女健康监测的潜力。

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引用次数: 0

Dual-strain engineering in Pd metallene for ultrasensitive tetracycline electrochemical biosensing 超灵敏四环素电化学生物传感用金属钯双应变工程

IF 10.5 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2026-05-01 Epub Date: 2026-01-27 DOI: 10.1016/j.bios.2026.118443

Yi Wen , Yinan Dong , Xuejie Shen , Xingyu Luo , Ziyi Wang , Ruirui Guo , Zhituo Geng , Peng Li , Yongping Huang , Xinyu Zhu , Jiatong Liu , Wangyang Fu , Zehui Li , Xiaoyan Zhang

Precise modulation of strain and electronic structure via atomic-level Mo and Cr co-doping in Pd metallene (MoCr-Pdene) significantly enhances electrocatalytic tetracycline (TC) sensing. Conventional Pd-based sensors suffer from weak TC affinity and sluggish kinetics due to surface inertness and biofouling. MoCr-Pdene overcomes these limitations through synergistic dual-strain effects, including compressive strain (from Cr) stabilizing the lattice and tensile strain (from Mo) redistributing surface charge. This strain-charge synergy shortens the Pd-O adsorption distance (1.82 Å), enhances adsorption energy (0.86 eV), and accelerates electron transfer, enabling an ultra-low detection limit of 27 nM, which represents ten-fold improvement over commercial Pd/C. The co-doped metallene exhibits exceptional stability (retaining 80 % of its signal after 120 days) and anti-biofouling capability in matrices. Importantly, MoCr-Pdene serves as a dual-functional platform for clinical diagnostics and environmental monitoring, quantitatively assessing TC biodegradation by black soldier fly larvae (BSFL) and gut microbiota (degradation rates: 97.96 % in frass, 90.67 % in BSFL). These results pioneer atomic-scale strain-electronic engineering for next-generation antibiotic sensing and sustainable bioremediation.

通过原子水平Mo和Cr共掺杂金属钯（MoCr-Pdene），精确调制应变和电子结构，显著增强电催化四环素（TC）传感。由于表面惰性和生物污染，传统的基于pd的传感器具有较弱的TC亲和力和缓慢的动力学。MoCr-Pdene通过协同的双应变效应克服了这些限制，包括压缩应变（来自Cr）稳定晶格和拉伸应变（来自Mo）重新分配表面电荷。这种菌株-电荷协同作用缩短了Pd- o的吸附距离（1.82 Å），提高了吸附能（0.86 eV），并加速了电子转移，实现了27 nM的超低检测限，比商用Pd/C提高了10倍。共掺杂的金属烯在基质中表现出优异的稳定性（120天后仍能保持80% %的信号）和抗生物污染能力。重要的是，MoCr-Pdene作为临床诊断和环境监测的双重功能平台，定量评估黑兵蝇幼虫（BSFL）和肠道微生物群对TC的生物降解（降解率：在草中为97.96 %，在BSFL中为90.67 %）。这些结果开创了下一代抗生素传感和可持续生物修复的原子尺度菌株电子工程。

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引用次数: 0

An ultrasensitive FEN1-aided LCR-electrochemical biosensor enables detection of BCR/ABL1 fusion transcripts in clinical samples for early diagnosis and minimal residual monitoring of CML 一种超灵敏的fen1辅助lcr -电化学生物传感器可以检测临床样品中的BCR/ABL1融合转录物，用于CML的早期诊断和最小残留监测

IF 10.5 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2026-05-01 Epub Date: 2026-01-27 DOI: 10.1016/j.bios.2026.118442

Liang-Yong Yang , Guang-Hui Mei , Xin-Ru Lin , Cai-Hong Wang , Rui-Long Lan , Chen-Liu Ye , Yan-Fang Xu , Xin-Hua Lin , Jin-Yuan Chen

Ligase chain reaction (LCR) is a promising nucleic acid amplification technique widely applied in molecular diagnosis. However, a critical limitation of LCR is target-independent amplification, primarily caused by the blunt-end ligation activity of thermophilic DNA ligases, which results in false positives and compromises sensitivity. To overcome this challenge, we developed a thermostable flap endonuclease 1 (FEN1)-aided LCR (FALCR) employing DNA probes with non-phosphorylated sticky ends. In each amplification cycle, FEN1 selectively cleaves the 5′ DNA flap of the downstream probe in the presence of a DNA template, generating 5′-phosphorylated DNA nicks that enable ligation. In the absence of a DNA template, the non-phosphorylated sticky ends of the probes prevent ligation, effectively eliminating nonspecific amplification. We further integrated FALCR with a magnetic platform-based electrochemical biosensor (FA-eLCR), achieving ultrasensitive detection of DNA targets with a limit of detection (LOD) of 0.1 aM and a dynamic range spanning 10 aM to 10 fM. Furthermore, the designing of a duplex FALCR enabled the detection of BCR/ABL1^p210 comprising e13a2/e14a2/both isoforms in a single assay. Finally, the duplex FA-eLCR successfully detected the BCR/ABL1^p210 transcripts in patients with chronic myeloid leukemia (CML) and monitored the change of transcripts during treatment, with 100 % concordance to reverse transcription-quantitative polymerase chain reaction, highlighting its high potential in the early diagnosis and minimal residual disease (MRD) monitoring of CML. This work presents a novel strategy to address nonspecific amplification in LCR and introduces a cost-effective, highly sensitive platform for molecular diagnosis in clinical setting.

连接酶链反应（LCR）是一种具有广泛应用前景的核酸扩增技术。然而，LCR的一个关键限制是不依赖靶标的扩增，这主要是由嗜热DNA连接酶的钝端连接活性引起的，这会导致假阳性和降低灵敏度。为了克服这一挑战，我们利用具有非磷酸化粘性末端的DNA探针开发了一种耐热皮瓣内切酶1 （FEN1）辅助LCR （FALCR）。在每个扩增周期中，FEN1在DNA模板存在的情况下选择性地切割下游探针的5 ‘ DNA瓣，产生5 ’磷酸化的DNA缺口，从而实现连接。在没有DNA模板的情况下，探针的非磷酸化粘性末端防止结扎，有效地消除了非特异性扩增。我们进一步将FALCR与基于磁性平台的电化学生物传感器（FA-eLCR）集成在一起，实现了对DNA靶标的超灵敏检测，检测限（LOD）为0.1 aM，动态范围为10 aM至10 fM。此外，双相FALCR的设计使BCR/ABL1p210包含e13a2/e14a2/两种亚型的检测成为可能。最后，双链FA-eLCR成功检测了慢性髓性白血病（CML）患者的BCR/ABL1p210转录本，并监测了治疗过程中转录本的变化，与逆转录-定量聚合酶链反应的一致性为100 %，突出了其在CML早期诊断和微小残留病（MRD）监测中的巨大潜力。这项工作提出了一种解决LCR非特异性扩增的新策略，并引入了一种具有成本效益，高度敏感的临床分子诊断平台。

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引用次数: 0

A novel self-powered electrochemical sensor based on zinc-air battery for thrombin detection 一种基于锌-空气电池的自供电电化学凝血酶检测传感器

IF 10.5 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2026-05-01 Epub Date: 2026-01-24 DOI: 10.1016/j.bios.2026.118429

Zhengchun Sun , Weili Zhang , Junhong Liu , Shina Du , Yuebo Wang , Zhongfang Li , Xueliang Niu , Wei Sun

As an emerging sensing mode, self-powered electrochemical sensor (SPES) has gained much attention for its advantages of without external power supply and portable detection. However, developing SPES with enhanced output signals and anti-influence characteristics is still a challenge. To address these issues, herein, a novel zinc-air battery (ZAB)-based SPES with aptamer as a specific recognition element was constructed. In this case, bimetallic Fe/Ni and N co-doping carbon and in-situ grown carbon nanotubes (Fe-Ni/N-C/CNT) was synthesized by mixing Fe, Ni precursors with previously prepared ZIF-8. Under neutral conditions, Fe-Ni/N-C/CNT exhibits a high half-wave potential of 0.831 V (vs. RHE) and the ZAB assembled with Fe-Ni/N-C/CNT as an air cathode generates a considerable maximum peak power density (P_max) of 84.1 mW cm⁻². As a concept, thrombin (TB) was selected as a detection target, a “signal-off” SPES based on the interfacial steric hindrance effect generated by aptamers structure bending while capturing the target was constructed with P_max as a sensing signal. P_max values of the prepared ZAB-based SPES show a good linear relationship with logarithm of TB concentrations, accompanied by a linear range from 2 × 10⁻⁴ nM to 2 × 10⁻² nM, and a detection limit of 66.7 fM (LOD). The constructed ZAB-based SPES shows good anti-interference ability, stability, and reproducibility, making it feasible and efficient in practical applications. Furthermore, this SPES strategy displays a promising generalizability for other targets detection by replacing the aptamers modified on the sensing cathode surface.

自供电电化学传感器（SPES）作为一种新兴的传感方式，以其无需外接电源和检测便携等优点而备受关注。然而，开发具有增强输出信号和抗影响特性的spe仍然是一个挑战。为了解决这些问题，本文构建了一种新的基于锌空气电池（ZAB）的SPES，并以适配体作为特定的识别元素。在这种情况下，通过将Fe， Ni前驱体与先前制备的ZIF-8混合，合成了双金属Fe/Ni和N共掺杂碳纳米管和原位生长碳纳米管（Fe-Ni/N- c /CNT）。在中性条件下，Fe-Ni/N-C/CNT表现出0.831 V（相对于RHE）的高半波电位，与Fe-Ni/N-C/CNT组装的ZAB作为空气阴极产生了相当大的峰值功率密度（Pmax），为84.1 mW cm−2。选取凝血酶（TB）作为检测靶点，利用适体结构弯曲产生的界面位阻效应构建了“信号关闭”SPES，并以Pmax作为传感信号捕获靶点。所制备的zab基SPES的Pmax值与TB浓度的对数呈良好的线性关系，线性范围为2 × 10−4 nM ~ 2 × 10−2 nM，检出限为66.7 fM （LOD）。基于zab构建的SPES具有良好的抗干扰能力、稳定性和重复性，在实际应用中具有可行性和高效性。此外，通过替换传感阴极表面修饰的适体，该策略在其他目标检测中显示出很好的推广前景。

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引用次数: 0

Dual CRISPR/Cas-driven amplification-free surface-enhanced Raman scattering biosensor combined with a smartphone for simultaneous detection of total and live target bacteria 双CRISPR/ cas驱动的无扩增表面增强拉曼散射生物传感器与智能手机相结合，用于同时检测总细菌和活目标细菌

IF 10.5 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2026-05-01 Epub Date: 2026-01-27 DOI: 10.1016/j.bios.2026.118446

Ruibao Jiao , Qun Ni , Ruirui Zhao , Xiaofan Zhu , Lixia Wang , Jingran Jiao , Han Jiang , Qian Wu , Shuang Yao , Li Yao , Kaiyong Liu , Panzhu Qin

Simultaneous detection of total and live counts of target bacteria is significant but challenging. To address this challenge, here we proposed a dual CRISPR/Cas-driven amplification-free surface-enhanced Raman scattering (SERS) biosensor, termed cc-SERS. The biosensor was constructed based on the property that DNA remains stable while some RNA degrades rapidly after bacterial death. Briefly, the target DNA and RNA from live bacteria could activate both CRISPR/Cas12a and CRISPR/Cas13a, while dead bacteria could only activate CRISPR/Cas12a through the target DNA. In the absence of the target bacteria, neither CRISPR/Cas12a nor CRISPR/Cas13a could be activated. Therefore, the characteristic Raman signal at 1079 cm⁻¹ generated by the target DNA-activated CRISPR/Cas12a indicated the presence of the target bacteria (the sum of dead and live), while the characteristic Raman signal at 593 cm⁻¹ produced by the target RNA-activated CRISPR/Cas13a indicated the presence of the live target bacteria. With this unique signaling pattern, the biosensor is capable of detecting both total and live target bacteria in a single tube with a detection limit of ∼10 CFU/mL. The introduction of a rapid pre-processing procedure and a smartphone-assisted portable Raman spectrometer enabled the entire process to be completed in the field within 45 min. Thanks to the excellent programmability of CRISPR/Cas systems, the biosensor has been successfully applied to the detection of Staphylococcus aureus and Cronobacter sakazakii, respectively. As a proof-of-concept, this work opens a promising avenue for the simultaneous detection of total and live target bacteria.

同时检测目标细菌的总数和活计数是重要的，但具有挑战性。为了解决这一挑战，我们提出了一种双CRISPR/ cas驱动的无扩增表面增强拉曼散射（SERS）生物传感器，称为cc-SERS。该生物传感器是基于细菌死亡后DNA保持稳定而一些RNA迅速降解的特性而构建的。简而言之，活菌的靶DNA和RNA可以同时激活CRISPR/Cas12a和CRISPR/Cas13a，而死菌只能通过靶DNA激活CRISPR/Cas12a。在没有目标细菌的情况下，CRISPR/Cas12a和CRISPR/Cas13a都不能被激活。因此，靶dna激活的CRISPR/Cas12a产生的1079 cm−1处的特征拉曼信号表明存在目标菌（死菌和活菌之和），而靶rna激活的CRISPR/Cas13a产生的593 cm−1处的特征拉曼信号表明存在活的目标菌。凭借这种独特的信号模式，该生物传感器能够在单管中检测总目标细菌和活目标细菌，检测限为~ 10 CFU/mL。快速预处理程序和智能手机辅助便携式拉曼光谱仪的引入使整个过程在45 min内完成。由于CRISPR/Cas系统出色的可编程性，该生物传感器已分别成功应用于金黄色葡萄球菌和阪崎克罗诺杆菌的检测。作为概念验证，这项工作为同时检测总目标细菌和活目标细菌开辟了一条有希望的途径。

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引用次数: 0

Photoelectrochemical and colorimetric dual-mode immunosensor based on Cu-BTC-stabilized hemin nanozyme for point-of-care detection of neuronal-specific enolase in serum 基于cu - btc稳定血红蛋白纳米酶的光电化学和比色双模免疫传感器用于即时检测血清中神经元特异性烯醇化酶

IF 10.5 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2026-05-01 Epub Date: 2026-01-23 DOI: 10.1016/j.bios.2026.118439

Ke Xiao , Sitong Huang , Jia Sun , Zhen Liu , Leixia Meng

As a primary cause of global mortality, cerebral stroke (CS) necessitates prompt diagnosis, where detecting neuron-specific enolase (NSE) rapidly and accurately is key to effective intervention. To resolve this issue, a novel dual-mode photoelectrochemical and colorimetric (PEC-CL) immunosensor was proposed for point-of-care detection of NSE. The sensing platform utilizes magnetic Fe₃O₄@Bi₂S₃ nanoparticles (NPs) functionalized with primary antibody as PEC-active capture probes and hemin-encapsulated Cu-based metal-organic framework (Cu-BTC@hemin) conjugated with secondary antibody as multi-functional signal probes. A specific dual-signal transduction is triggered by the formation of the sandwich immunocomplex upon NSE binding. This process involves electron transfer from hemin to Bi₂S₃, leading to enhanced attenuation of the PEC signal, and CL response from hemin of supernatant mediated catalytic of 3,3′,5,5′-tetramethylbenzidine (TMB) oxidation. This sensor demonstrates outstanding performance and inherent self-verification capabilities. Furthermore, the dual-mode sensor displays robust consistency in NSE quantification across clinical samples. Its demonstrated excellence in specificity, reproducibility, and robustness in complex serum matrices establishes a solid groundwork for next-generation point-of-care CS diagnostics. Moreover, it offers a generalizable blueprint for creating multimodal biosensors for other biomarkers, thereby accelerating progress accelerating progress in precision medicine for cerebrovascular diseases.

作为全球死亡的主要原因，脑卒中（CS）需要及时诊断，其中快速准确地检测神经元特异性烯醇化酶（NSE）是有效干预的关键。为了解决这一问题，提出了一种新的双模光电化学和比色（PEC-CL）免疫传感器，用于即时检测NSE。传感平台采用一抗功能化的磁性Fe3O4@Bi2S3纳米粒子（NPs）作为pec活性捕获探针，采用二抗偶联的血红素包封铜基金属有机框架（Cu-BTC@hemin）作为多功能信号探针。特异的双信号转导是由NSE结合后三明治免疫复合物的形成触发的。这一过程包括从hemin到Bi2S3的电子转移，导致PEC信号的衰减增强，并且上清中hemin的CL响应介导了3,3 ',5,5 ' -四甲基联苯胺（TMB）氧化。该传感器具有出色的性能和固有的自我验证能力。此外，双模传感器在临床样本的NSE量化中显示出强大的一致性。它在复杂血清基质中表现出卓越的特异性、可重复性和稳健性，为下一代即时诊断CS奠定了坚实的基础。此外，它为为其他生物标志物创建多模态生物传感器提供了一个通用的蓝图，从而加速了脑血管疾病精准医学的进展。

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引用次数: 0