Environment International最新文献

{"title":"A national-scale assessment of hospital contributions to chemical loads in wastewater and the implications for wastewater-based epidemiology","authors":"Neil Andrew Byrnes,&nbsp;Reshma Silvester,&nbsp;Gareth Cross,&nbsp;Andrew J. Weightman,&nbsp;Davey L. Jones,&nbsp;Barbara Kasprzyk-Hordern","doi":"10.1016/j.envint.2026.110476","DOIUrl":"10.1016/j.envint.2026.110476","url":null,"abstract":"<div><div>Wastewater-based epidemiology (WBE) is an established approach for assessing population health through the quantification of pharmaceuticals and lifestyle chemicals in wastewater. As hospitals are a potential point source for these chemical indicators (CIs), assessment of population health within hospitals may be possible via WBE while hospitals’ chemical contributions have the potential to impact community wide WBE assessments. Untreated hospital wastewater and corresponding community influent wastewater were collected from six sites across Wales through a national sampling campaign capturing 18 % of the Welsh population and 23 % of hospital in-patients nationally in summer 2023. Using liquid chromatography and targeted mass spectrometry, the mass loads of over 50 CIs were quantified. An exploratory analysis of sewer catchment parameters and hospital parameters identified possible trends. From these insights, further examination of data from published literature demonstrated a statistically significant inverse relationship between catchment population and hospitals’ average contribution to influent wastewater flow. This suggests that hospitals located in smaller wastewater catchments may have more influence on the total CI load in influent wastewater. Across all CIs assessed, antimicrobials were the only chemical class to have a substantial influence on influent wastewater composition. The median percent contribution of sulfamethoxazole, trimethoprim, and fluconazole across studied sites ranged from 5 to 50 %, 6–66 %, and 2–33 %, respectively. Hospital contributions of antimicrobials can meaningfully impact the conclusions from WBE assessments of community antimicrobial use. These findings may help identify priority sites for future interventions to reduce antimicrobial loads in wastewater environments and their potential contribution to antimicrobial resistance.</div></div>","PeriodicalId":308,"journal":{"name":"Environment International","volume":"215 ","pages":"Article 110476"},"PeriodicalIF":10.2,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148838735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Effects of ambient fine particulates, nitrogen dioxide, and ozone on maturation of functional brain networks across early adolescence” [Environ. Int. 177 (2023) 108001]","authors":"Devyn L. Cotter,&nbsp;Claire E. Campbell,&nbsp;Kirthana Sukumaran,&nbsp;Rob McConnell,&nbsp;Kiros Berhane,&nbsp;Joel Schwartz,&nbsp;Daniel A. Hackman,&nbsp;Hedyeh Ahmadi,&nbsp;Jiu-Chiuan Chen,&nbsp;Megan M. Herting","doi":"10.1016/j.envint.2026.110417","DOIUrl":"10.1016/j.envint.2026.110417","url":null,"abstract":"","PeriodicalId":308,"journal":{"name":"Environment International","volume":"215 ","pages":"Article 110417"},"PeriodicalIF":10.2,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148629183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Severe winter PM2.5 pollution in remote Canadian mountain communities: Insights into the role of condensable particulate matter under subzero conditions","authors":"Qinchu Fan,&nbsp;Xiaohong Yao,&nbsp;Leiming Zhang","doi":"10.1016/j.envint.2026.110472","DOIUrl":"10.1016/j.envint.2026.110472","url":null,"abstract":"<div><div>Fine particulate matter (PM_2.5) remains a major air-quality and public-health concern despite increasingly stringent emission regulations. While PM_2.5 pollution has been extensively studied in urban and industrial regions, severe episodes in remote communities remain poorly characterized. Using observations from the Canadian National Air Pollution Surveillance (NAPS) network, we identified severe PM_2.5 pollution episodes in two remote mountain communities in British Columbia, Canada. These episodes were associated primarily with residential space-heating emissions during winter and episodic wildfire smoke impacts during summer. Wintertime daily PM_2.5 exceedance frequencies varied substantially among years, reaching seasonal maxima of approximately 30%. Winter exceedances exhibited recurrent nocturnal episodes of rapid PM_2.5 accumulation under low wind speeds, occurring at consistent times of day but with highly variable hourly growth rates. This behavior is consistent with a dominant contribution from primary emissions, particularly condensable particulate matter (CPM) formed during the early evolution of residential-combustion plumes under subzero conditions. Regression analyses suggested that CPM contributions may increase by as much as ∼100 μg m⁻³ as ambient temperature decreased from 0 °C to −30 °C. Paired PM_2.5 and PM₁₀ measurements further revealed substantial evaporation of semi-volatile CPM during plume transport, implying that wintertime PM_2.5 variability was governed largely by the accumulation and partial evaporation of primary particulate matter. In the absence of concurrent PM_2.5 chemical composition measurements at the two study sites, chemical composition data from other NAPS sites were used to provide supplementary evidence for the analysis. The findings obtained from this study highlight the importance of improving CPM quantification under subzero conditions for effective air-quality management in cold-climate communities.</div></div>","PeriodicalId":308,"journal":{"name":"Environment International","volume":"215 ","pages":"Article 110472"},"PeriodicalIF":10.2,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148853314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Repeated oral exposure to polyethylene microplastics induces thyroid toxicity in rats: Evidence from in vivo toxicokinetics and multi-omics analyses","authors":"Tingting Sun,&nbsp;Xinyu Yang,&nbsp;Zhixiong Zhong,&nbsp;Shicong Yang,&nbsp;Zhibiao Huang,&nbsp;Guoqun He,&nbsp;Jieling Wang,&nbsp;Yayi Huang,&nbsp;Yuenan Wang,&nbsp;Yujia Jiang,&nbsp;Xianglin Chen,&nbsp;Jiaming Du,&nbsp;Jianbin Tan,&nbsp;Wenjuan Zhang,&nbsp;Min Zhao","doi":"10.1016/j.envint.2026.110499","DOIUrl":"10.1016/j.envint.2026.110499","url":null,"abstract":"<div><div>The widespread use and non-biodegradable nature of polyethylene microplastics (PE-MPs) have prompted significant public concern about their safety. However, the mechanisms underlying their adverse effects on human health remain largely elusive. Male rats were orally administered 34-μm PE-MPs via daily gavage for 28 consecutive days, with three dose groups set at 0.6, 6.0, and 60.0 mg/kg body weight (bw), respectively. The results showed that exposure to PE-MPs induced a decrease in serum triiodothyronine (T3) levels, accompanied by an increased incidence of thyroid follicular dilation, both of which displayed a distinct dose–response relationship. The 28-day oral toxicity study in male rats identified a no-observed-adverse-effect level (NOAEL) of 0.6 mg/kg bw and a lowest-observed-adverse-effect level (LOAEL) of 6.0 mg/kg bw for PE-MPs, corresponding to approximately 10 and 100 times the estimated daily human intake of microplastics, respectively. Toxicokinetics revealed that PE-MPs were absorbed and markedly accumulated in major organs, and the organ burdens correlated strongly with the severity of thyroid toxicity. Integrative multi-omics analysis implied that PE-MPs exposure altered the expression of thyroid toxicity-related genes <em>Ddit3</em>, <em>Zbtb16</em> and the levels of associated metabolites. This study provides exploratory experimental evidence identifying the thyroid as a target organ for PE-MPs-induced toxicity. It should be noted that conclusions drawn from multi-omics and toxicokinetic analyses are limited by the relatively small sample size in the present work. Further validation with larger sample sizes is required in future studies. These findings contribute valuable<!--> <!-->data to human health risk assessment and regulatory oversight of PE-MPs.</div></div>","PeriodicalId":308,"journal":{"name":"Environment International","volume":"215 ","pages":"Article 110499"},"PeriodicalIF":10.2,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148878607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cross-sectional and longitudinal relationships between exposure to polychlorinated biphenyls and predicted 10-year atherosclerotic cardiovascular disease risk: Integrated epidemiological and bioinformatics analysis","authors":"Yuxin Yao,&nbsp;Bingdong Chen,&nbsp;Jianghong Dai,&nbsp;Ruyi Liang,&nbsp;Jiake Zhang,&nbsp;Mengyi Wang,&nbsp;Qiyou Tan,&nbsp;Xuejie Ding,&nbsp;Yanjun Guo,&nbsp;Dongming Wang,&nbsp;Bin Wang,&nbsp;Haoyu Yin,&nbsp;Bingxin Shang,&nbsp;Xiaojie You,&nbsp;Jixuan Ma,&nbsp;Min Zhou,&nbsp;Weihong Chen","doi":"10.1016/j.envint.2026.110493","DOIUrl":"10.1016/j.envint.2026.110493","url":null,"abstract":"<div><div>Polychlorinated biphenyls (PCBs) are persistent organic pollutants of global concern, yet their associations with predicted cardiovascular risk remain unclear. In this prospective Wuhan-Zhuhai cohort study, concentrations of seven serum PCBs (PCB-28, 52, 101, 118, 138, 153, and 180) were measured using GC–MS/MS. The validated China-PAR equation was used to calculate predicted 10-year atherosclerotic cardiovascular disease (ASCVD) risk, and urinary 8-<em>iso</em>-prostaglandin F2α (8-<em>iso</em>-PGF_2α) served as a marker of oxidative stress. Multivariable linear and logistic regression models were fitted to evaluate PCB-related differences in continuous predicted risk and predicted high-risk status. Cross-sectionally, each one-unit increase in ln-transformed PCB-118, PCB-138, PCB-153, and ΣPCB was associated with 1.249-, 1.253-, 1.457-, and 1.829-percentage-point higher predicted 10-year ASCVD risk, respectively (all <em>P</em> &lt; 0.05). The associations for PCB-118, PCB-138, and PCB-153 remained significant after FDR correction. Longitudinally, persistent high PCB-118 exposure was associated with a 0.510 percentage-point greater increase in predicted 10-year ASCVD risk (95 % confidence interval [CI]: 0.023–0.996, <em>P</em> &lt; 0.05) and a higher risk of transitioning to predicted high-risk status (RR = 1.432, 95 % CI: 1.012–2.027, <em>P</em> &lt; 0.05) compared with persistent low exposure, although neither association remained significant after FDR correction. Exploratory mediation analysis suggested that urinary 8-<em>iso</em>-PGF_2α was statistically consistent with partial indirect pathways for the associations, with estimated indirect proportions ranging from 3.29 % to 11.62 %. Database-derived bioinformatics analyses highlighted <em>TNF</em>/<em>NF-κB/IL-17</em> pathways and identified <em>IL-6</em>, <em>TNF</em>, <em>IL-1β</em>, and <em>ICAM1</em> as candidate hub genes. Overall, several PCB measures were cross-sectionally associated with higher predicted ASCVD risk, whereas longitudinal evidence was limited and was strongest for persistent high PCB-118 exposure. Exploratory mediation and bioinformatics findings highlighted possible oxidative-stress and inflammation-related pathways that require further validation.</div></div>","PeriodicalId":308,"journal":{"name":"Environment International","volume":"215 ","pages":"Article 110493"},"PeriodicalIF":10.2,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148883850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Occurrence, mixture neurotoxicity risk, and prioritization of neonicotinoid and diamide insecticides in urban residential soils","authors":"Zuhai Chen,&nbsp;Jiajun Cao,&nbsp;Bingyi Fu,&nbsp;Menglian Zeng,&nbsp;Yiqian Luo,&nbsp;Bingjun Lu,&nbsp;Shaochong Liu,&nbsp;Yi Chen","doi":"10.1016/j.envint.2026.110507","DOIUrl":"10.1016/j.envint.2026.110507","url":null,"abstract":"<div><div>The widespread use of neonicotinoid (NEO) and diamide (DAI) insecticides has led to their frequent environmental occurrence, yet their presence and associated health risks in urban residential soils remain poorly understood. This study collected 200 residential soils from 10 provinces of China, revealing widespread detection of both NEOs and DAIs. Concentrations of ∑₁₁NEOs and ∑₈DAIs ranged from 0.31 − 1878 ng/g and ND − 6.27 ng/g, respectively, with imidacloprid (IMI) and chlorantraniliprole (CTP) as the dominant compounds. Both NEOs and DAIs exhibited marked spatial variability across cities, with relatively higher concentrations observed in several northern cities. While conventional exposure assessment indicated negligible health risks via soil-related pathways, a field-informed computational framework integrating mixture quantitative structure–activity relationship (QSAR) and toxicological priority index (ToxPi) was developed to characterize predicted mixture neurotoxicity potential and identify priority compounds. QSAR modeling indicated that DAI mixtures exhibited higher predicted mixture neurotoxicity potential than NEO mixtures despite substantially lower environmental concentrations, whereas NEO mixtures were characterized by higher residue levels and exposure-weighted intake. ToxPi analysis identified IMI and CTP as top-ranked priority compounds within NEOs and DAIs, respectively. This study advances mixture-level risk prioritization of co-occurring insecticides by integrating exposure characterization with QSAR-based neurotoxicity predictions, providing a complementary approach to conventional concentration-based monitoring.</div></div>","PeriodicalId":308,"journal":{"name":"Environment International","volume":"215 ","pages":"Article 110507"},"PeriodicalIF":10.2,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148928473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prenatal and childhood exposure to mixtures of endocrine-disrupting chemicals in association with measures of adiposity in childhood and adolescence","authors":"Nuria Güil-Oumrait,&nbsp;Vishal Midya,&nbsp;Sandra India-Aldana,&nbsp;Augusto Anguita-Ruiz,&nbsp;Léa Maitre,&nbsp;Mariona Bustamante,&nbsp;Parisa Montazeri,&nbsp;Jose Urquiza,&nbsp;Line Småstuen Haug,&nbsp;Anne Lise Brantsæter,&nbsp;Barbara Heude,&nbsp;John Wright,&nbsp;Tiffany C. Yang,&nbsp;Rosemary R.C. McEachan,&nbsp;Euripides G. Stephanou,&nbsp;Regina Grazuleviciene,&nbsp;Rémy Slama,&nbsp;Leda Chatzi,&nbsp;Martine Vrijheid,&nbsp;Damaskini Valvi","doi":"10.1016/j.envint.2026.110495","DOIUrl":"10.1016/j.envint.2026.110495","url":null,"abstract":"<div><h3>Background</h3><div>Childhood obesity is a global public health crisis. Endocrine-disrupting chemicals (EDCs) − widespread pollutants that interfere with hormonal and metabolic systems − may contribute to excessive adiposity. The combined, long-term, and mechanistic effects of multi-class EDC mixtures remain unexplored.</div></div><div><h3>Methods</h3><div>We analyzed 1301 mother–child pairs from the Human Early Life Exposome (HELIX) project, spanning six European cohorts. Prenatal and childhood exposures to 45 EDCs across nine chemical classes were measured in maternal and child biospecimens. Thirty-six plasma proteins were quantified in childhood using targeted proteomic panels. Adiposity outcomes in childhood (6–11 years) and adolescence (12–18 years) included BMI z-scores, waist circumference, and body fat percentage. We applied penalized Lasso-type Bayesian Weighted Quantile Sum Regression to assess mixtures, a signed iterative random forest for interactions, and mediation analyses for biological pathways.</div></div><div><h3>Results</h3><div>Prenatal metal and organochlorine pesticide mixtures were associated with higher BMI z-scores in childhood and adolescence and 47–48 % higher odds of overweight. Prenatal PFAS exposure was associated with higher body-fat percentage in both periods. Childhood phenol and high-molecular-weight phthalate exposures were associated with higher adolescent zBMI, whereas parabens and persistent compounds showed inverse associations. Childhood metal exposures were associated with higher body-fat percentage and overweight odds. An inflammatory-metabolic protein pattern mediated ∼ 30 % of the prenatal metal-adolescent zBMI association.</div></div><div><h3>Conclusions</h3><div>Early-life exposure to EDC mixtures may reprogram metabolic and inflammatory pathways, predisposing children to obesity that persists into adolescence. These findings provide mechanistic evidence linking chemical exposures to the global obesity epidemic and underscore the urgent need for policies preventing EDC exposure during critical developmental periods.</div></div>","PeriodicalId":308,"journal":{"name":"Environment International","volume":"215 ","pages":"Article 110495"},"PeriodicalIF":10.2,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148895550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retraction notice to “High-throughput screening of ancient forest plant extracts shows cytotoxicity towards triple-negative breast cancer” [Environ. Int. 181 (2023) 108279]","authors":"Yiyang Li,&nbsp;Nyuk Ling Ma,&nbsp;Huiling Chen,&nbsp;Jiateng Zhong,&nbsp;Dangquan Zhang,&nbsp;Wanxi Peng,&nbsp;Su Shiung Lam,&nbsp;Yafeng Yang,&nbsp;Xiaochen Yue,&nbsp;Lijun Yan,&nbsp;Ting Wang,&nbsp;Bjarne Styrishave,&nbsp;Tomasz Maciej Ciesielski,&nbsp;Christian Sonne","doi":"10.1016/j.envint.2026.110443","DOIUrl":"10.1016/j.envint.2026.110443","url":null,"abstract":"","PeriodicalId":308,"journal":{"name":"Environment International","volume":"215 ","pages":"Article 110443"},"PeriodicalIF":10.2,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148885820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nitrogen-containing organic compounds in household solid-fuel combustion PM and residential urinary inflammatory and targeted amino-acid profiles: an exploratory personal exposure study","authors":"Yunxuan Gu,&nbsp;Hongmei Xu,&nbsp;Sulin Chen,&nbsp;Yuqi Pang,&nbsp;Bei Han,&nbsp;Lu Bai,&nbsp;Steven Sai Hang Ho,&nbsp;Jian Sun,&nbsp;Zhenxing Shen,&nbsp;Junji Cao","doi":"10.1016/j.envint.2026.110491","DOIUrl":"10.1016/j.envint.2026.110491","url":null,"abstract":"<div><div>Nitrogen-containing organic compounds (NOCs) are important constituents of fine particulate matter emitted from household solid-fuel combustion, yet their personal-exposure characteristics and potential biological relevance remain insufficiently understood. We conducted an exploratory cross-sectional study among 15 rural homemakers, including 12 solid-fuel users and three electric-heating controls. Personal breathing-zone PM_2.5 and PM_0.25 samples were collected during two consecutive 24-h periods, and six classes of filter-collected PM-associated NOCs were quantified. Urinary inflammatory and oxidative-stress-related biomarkers and a targeted panel of 62 amino-acid-related metabolites were also analyzed. Personal exposure concentrations of the measured NOC classes were substantially higher in solid-fuel households than in electric-heating households, with particularly pronounced differences in free amino acids (FAAs) and C6-C20 alkyl nitriles. Urea dominated the total measured NOC mass, whereas FAAs constituted the largest non-urea fraction in the solid-fuel group. Correlation and exploratory mixture-response analyses indicated that FAAs and alkyl nitriles were the NOC classes most consistently associated with urinary C-reactive protein and vascular endothelial growth factor. Targeted urinary amino-acid profiling identified lower urinary concentrations of 4-hydroxyphenyllactic acid and methylguanidine in solid-fuel users based on nominal criteria, together with a borderline reduction in serotonin. Exploratory KEGG analysis further organized the measured metabolites into broad amino-acid-related biochemical categories, but was not interpreted as evidence of pathway activation or impairment. Overall, household solid-fuel use was associated with an NOC-rich personal PM exposure profile and selected urinary biomarker and targeted amino-acid-related patterns. Given the small and imbalanced sample and cross-sectional design, these findings should be interpreted as hypothesis-generating associations rather than confirmed biomarkers, causal effects, or mechanistic evidence.</div></div>","PeriodicalId":308,"journal":{"name":"Environment International","volume":"215 ","pages":"Article 110491"},"PeriodicalIF":10.2,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148878589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PFAS disrupt adult hippocampal neurogenesis and synaptic remodeling associated with NOX2/ROS/Ferroptosis activation","authors":"Xingcheng Li,&nbsp;Xiao Li,&nbsp;Cimei Li,&nbsp;Lingfen Cao,&nbsp;Siteng Li,&nbsp;Xiaoping Wang,&nbsp;Kangchao Du,&nbsp;Yaoyao Cui,&nbsp;Yuxiang Ma,&nbsp;Xu Wang,&nbsp;Xing Xing,&nbsp;Huigen Feng,&nbsp;Ciqing Yang,&nbsp;Xianwei Wang,&nbsp;Cixia Li","doi":"10.1016/j.envint.2026.110503","DOIUrl":"10.1016/j.envint.2026.110503","url":null,"abstract":"<div><div>Per- and polyfluoroalkyl substances (PFAS) accumulate in the hippocampus, yet their effects on adult hippocampal neurogenesis (AHN) remain unclear. In this study, adult male mice were orally exposed to PFOA or GenX (2 or 10 mg/kg/day) for 28 days. Behavioral performance, AHN, synaptic remodeling, microglial morphology, and redox status were evaluated using behavioral assays, Nissl and Golgi staining, BrdU immunolabeling, microglial morphometric analyses, and Western blotting. PFOA exposure and high-dose GenX exposure reduced time spent and distance traveled in the center area of the open field and impaired spatial learning and memory performance in the Morris water maze, as indicated by increased escape latency and fewer platform crossings. In contrast, PFOA and low-dose GenX exposure reduced exploration of the open arms in the elevated plus maze. PFOA and GenX reduced dendritic spine density with fewer mushroom/thin spines and more stubby spines. PFOA preferentially decreased PSD95 (postsynaptic marker), whereas GenX reduced synaptophysin (presynaptic marker). In the subgranular zone (SGZ), survival and neuronal differentiation of neural stem cells were diminished, and asymmetric divisions of radial glia-like cells increased, suggesting stem-cell pool depletion. Microglia exhibited a hyper-ramified/bushy reactive phenotype. Hippocampal ROS/MDA rose, NOX2 components (GP91^phox/P22^phox) were upregulated, and ferroptosis defenses (SLC7A11/GLS2/GPX4) were downregulated. Collectively, PFOA and GenX disrupt hippocampal synaptic remodeling and AHN and are associated with increased oxidative stress and ferroptosis-related alterations. Differential pre- versus postsynaptic vulnerabilities may contribute to the distinct behavioral alterations observed following PFOA and GenX exposure. This work provides new insights into the neurotoxic effects of PFOA and GenX and identifies potential pathways involved in PFAS-induced hippocampal dysfunction.</div></div>","PeriodicalId":308,"journal":{"name":"Environment International","volume":"215 ","pages":"Article 110503"},"PeriodicalIF":10.2,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148883919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}