Pub Date : 2024-01-01Epub Date: 2023-11-27DOI: 10.56507/brev5387
Marc A Pizzimenti, Xiaoliu Zhang, Jessica E Goetz, Punam K Saha
Analytical strategies to interpret computed tomography data for the study of osteoporosis require gross anatomical specimens that closely approximate the bone marrow matrix while maintaining the structural integrity of the trabecular network. Here we investigated if paraffin, gelatin-matrix, or silicone polymer impregnated bone specimens maintained the trabecular network and bone density of specimens used for comparative studies in osteoporosis. Distal tibia specimens (n = 12) from cadaveric human legs were used for this study. Multi-row detector computed tomography (MDCT) scans were completed on each specimen, with selected specimens re-scanned using micro-computed tomography (μCT), at different times to evaluate trabecular integrity. Once scanned, bones were de-marrowed using a solvent-based procedure. De-marrowed specimens were then impregnated, under vacuum controlled conditions, with either a silicone polymer, gelatin matrix solution, or paraffin wax, and re-scanned. μCT analysis demonstrated a high Pearson correlation coefficient in trabecular bone network area density between the native and de-marrowed states (r = 0.99). Quantitatively, paraffin impregnated specimens demonstrated the highest congruence in MCDT bone volume fraction (r = 0.92) and trabecular network area (r = 0.94) measures. These results were more robust in the μCT data (bone volume fraction, r = 0.97; and trabecular network area, r = 0.99). Additionally, of the impregnating procedures, paraffin wax provided the best qualitative specimens for handling, storage, and processing time. These data suggest that paraffin demonstrates a space occupying matrix that effectively protects the trabecular network and maintains the ability to quantify important biomechanical measures for assessing bone integrity. The preserved specimens will serve as standard models for comparison when developing algorithms for studying osteoporosis.
{"title":"Preserving Trabecular Bone Structure in Gross Specimens with Paraffin Wax for the Study of Osteoporosis.","authors":"Marc A Pizzimenti, Xiaoliu Zhang, Jessica E Goetz, Punam K Saha","doi":"10.56507/brev5387","DOIUrl":"10.56507/brev5387","url":null,"abstract":"<p><p>Analytical strategies to interpret computed tomography data for the study of osteoporosis require gross anatomical specimens that closely approximate the bone marrow matrix while maintaining the structural integrity of the trabecular network. Here we investigated if paraffin, gelatin-matrix, or silicone polymer impregnated bone specimens maintained the trabecular network and bone density of specimens used for comparative studies in osteoporosis. Distal tibia specimens (n = 12) from cadaveric human legs were used for this study. Multi-row detector computed tomography (MDCT) scans were completed on each specimen, with selected specimens re-scanned using micro-computed tomography (μCT), at different times to evaluate trabecular integrity. Once scanned, bones were de-marrowed using a solvent-based procedure. De-marrowed specimens were then impregnated, under vacuum controlled conditions, with either a silicone polymer, gelatin matrix solution, or paraffin wax, and re-scanned. μCT analysis demonstrated a high Pearson correlation coefficient in trabecular bone network area density between the native and de-marrowed states (r = 0.99). Quantitatively, paraffin impregnated specimens demonstrated the highest congruence in MCDT bone volume fraction (r = 0.92) and trabecular network area (r = 0.94) measures. These results were more robust in the μCT data (bone volume fraction, r = 0.97; and trabecular network area, r = 0.99). Additionally, of the impregnating procedures, paraffin wax provided the best qualitative specimens for handling, storage, and processing time. These data suggest that paraffin demonstrates a space occupying matrix that effectively protects the trabecular network and maintains the ability to quantify important biomechanical measures for assessing bone integrity. The preserved specimens will serve as standard models for comparison when developing algorithms for studying osteoporosis.</p>","PeriodicalId":36740,"journal":{"name":"Journal of Plastination","volume":"36 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13295180/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148346648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Optimal temperature and duration for impregnation and curing of epoxy blocks for ultra-thin sections","authors":"","doi":"10.56507/awac9285","DOIUrl":"https://doi.org/10.56507/awac9285","url":null,"abstract":"","PeriodicalId":36740,"journal":{"name":"Journal of Plastination","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46640519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Implementation of a plastination laboratory in a public university in Northeastern Brazil: current challenges and future perspectives","authors":"","doi":"10.56507/wpbg4492","DOIUrl":"https://doi.org/10.56507/wpbg4492","url":null,"abstract":"","PeriodicalId":36740,"journal":{"name":"Journal of Plastination","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48820107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Vascular variations of the suprarenal gland: a cadaveric and postmortem study","authors":"","doi":"10.56507/dgzj6845","DOIUrl":"https://doi.org/10.56507/dgzj6845","url":null,"abstract":"","PeriodicalId":36740,"journal":{"name":"Journal of Plastination","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42068170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Influence of silicone viscosity on shrinkage during room and cold temperature impregnation","authors":"","doi":"10.56507/tqmh6049","DOIUrl":"https://doi.org/10.56507/tqmh6049","url":null,"abstract":"","PeriodicalId":36740,"journal":{"name":"Journal of Plastination","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44347120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Preparation of three-dimensional corrosion casts of the bronchovascular tree in man","authors":"","doi":"10.56507/fsnz3092","DOIUrl":"https://doi.org/10.56507/fsnz3092","url":null,"abstract":"","PeriodicalId":36740,"journal":{"name":"Journal of Plastination","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47021930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Fungal growth on a plastinated anatomical specimen","authors":"","doi":"10.56507/caxm7316","DOIUrl":"https://doi.org/10.56507/caxm7316","url":null,"abstract":"","PeriodicalId":36740,"journal":{"name":"Journal of Plastination","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70818714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Comparative analysis of possibilities for contactless monitoring of tissue condition in bodies undergoing long-term preservation","authors":"","doi":"10.56507/atma6059","DOIUrl":"https://doi.org/10.56507/atma6059","url":null,"abstract":"","PeriodicalId":36740,"journal":{"name":"Journal of Plastination","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46082584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
K. Hashida, K. Shirahata, J. Thompson, C. Liang, M. Takakusagi, K. Stenger, B. Lozanoff, S. Labrash, C. Rettenmeier, M. Gestewitz, U. Lee, S. Doll, S. Lozanoff
O. Melnyk, G. Tkach, Y. Guminskyi, V. Kovalchuk, Maria V Melnyk
: The body of N.I. Pirogov has been entombed in the Church of St. Nicolas in Vinnytsia, Ukraine, since 1881, when it was embalmed by Professor Vyvodtsev. During the re-embalming of the body of N.I. Pirogov in 2018, microbiological swabs from the body and its surroundings were taken and, among other things, skin, subcutaneous tissue, and muscle and bone tissues were taken for ultramicroscopic examination. Hyphae of fungi of the genus Penicillium citrinum were found at two sites during this examination. The occurrence of fungi can be explained by the already weakened immunity before death due to cancer, and its subsequent postmortem growth due to the non-use of formaldehyde and the placement of the body in a non-sterile and relatively humid environment of the tomb without regular control.
{"title":"Microbiological aspects of the examination of the 140-year-old embalmed body of N.I. Pirogov","authors":"O. Melnyk, G. Tkach, Y. Guminskyi, V. Kovalchuk, Maria V Melnyk","doi":"10.56507/nspx3350","DOIUrl":"https://doi.org/10.56507/nspx3350","url":null,"abstract":": The body of N.I. Pirogov has been entombed in the Church of St. Nicolas in Vinnytsia, Ukraine, since 1881, when it was embalmed by Professor Vyvodtsev. During the re-embalming of the body of N.I. Pirogov in 2018, microbiological swabs from the body and its surroundings were taken and, among other things, skin, subcutaneous tissue, and muscle and bone tissues were taken for ultramicroscopic examination. Hyphae of fungi of the genus Penicillium citrinum were found at two sites during this examination. The occurrence of fungi can be explained by the already weakened immunity before death due to cancer, and its subsequent postmortem growth due to the non-use of formaldehyde and the placement of the body in a non-sterile and relatively humid environment of the tomb without regular control.","PeriodicalId":36740,"journal":{"name":"Journal of Plastination","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46444329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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