Background
Tree nuts are common nutritional foods in daily life, but are also major allergenic foods, seriously affecting human health. Tree nut allergies are associated with allergens and epitopes. Thus, it is urgent to identify tree nut allergens and their epitopes, and to develop strategies to reduce allergenicity.
Scope and approach
The review summarizes the allergen composition, characteristics, and epitopes of ten types of tree nuts, especially almond, walnut, hazelnut, pecan and cashew. The impact of processing techniques on tree nut allergenicity is discussed. Future perspectives on addressing tree nut allergies are also provided.
Key findings and conclusions
Tree nut allergenicity is primarily determined by the properties, conformation, and epitopes of major allergens such as Pru du 6 for almond, Cor a 1, Cor a 8, Cor a 9 for hazelnut, Jug r 1∼Jug r 4 for walnut, Ana o 1∼Ana o 3 for cashew, Car i 1 and Car i 4 for pecan. The reduction of allergenicity in tree nuts mainly focuses on the thermal processing such as baking, boiling and autoclaving. However, thermal processing can generate advanced glycation end products, enhancing allergenic potential and harmful byproducts affecting human health. Thus, nonthermal processing (e.g., high-pressure, ultrasonication, fermentation, polyphenol modification, glycation, enzymatic hydrolysis, irradiation) and combined technologies should be comprehensively investigated to decrease tree nut allergenicity in the future. The correlation between “processing-structure-epitope-allergenicity” needs to be further elucidated to investigate the mechanism for decreasing tree nut allergenicity. Allergenicity alteration should be further verified through animal and clinical trials.


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