O. L. Shestopal, I. S. Zambriborshch, V. A. Traskovetskaya, O. A. Vasiliev, L. T. Babayants, M. S. Chekalova, O. A. Afinogenov
Aim. Obtaining homozygous linear material of winter soft wheat of different genetic origin (complex hybrids), which are resistant to different types of rust by androgenesis in vitro. Methods. In vitro culture of isolated anthers of wheat. The percentage of callus and regeneration of green plants for each genotype calculated as a percentage of the planted anthers. Methods of phytopathological assessment of resistance to complex of various diseases. Results. The differences in the frequency of сallus induction (from 0.99 to 16.26 % of planted anthers) and the ability to regenerate plants (from 0 to 3.05 % of planted anthers) in the process of androgenesiss in vitro of winter soft wheat were detected. Fertile regenerants in the anthers culture 5 out of 9 studied hybrids have been obtained. The effectiveness of the last process was different: from 12.5 to 60.0 % (an average of 29.41 %) from the received regenerants. The high degree (8–9 points) of the resistance of the obtained dihaploids to a complex of diseases was shown. Conclusions. Genotype-specific of microspores morphogenetic reactions of soft winter wheat in the process of androgenesis in vitro were revealed. The highest level of callus formation were shown for samples 120/20 and 132/20. 15 dihaploid lines with complex rust resistance and hard smut were obtained.
{"title":"Obtaining dihaploid lines of soft winter wheat with complex resistance to rust and hard smut by anther culture in vitro","authors":"O. L. Shestopal, I. S. Zambriborshch, V. A. Traskovetskaya, O. A. Vasiliev, L. T. Babayants, M. S. Chekalova, O. A. Afinogenov","doi":"10.7124/feeo.v32.1548","DOIUrl":"https://doi.org/10.7124/feeo.v32.1548","url":null,"abstract":"Aim. Obtaining homozygous linear material of winter soft wheat of different genetic origin (complex hybrids), which are resistant to different types of rust by androgenesis in vitro. Methods. In vitro culture of isolated anthers of wheat. The percentage of callus and regeneration of green plants for each genotype calculated as a percentage of the planted anthers. Methods of phytopathological assessment of resistance to complex of various diseases. Results. The differences in the frequency of сallus induction (from 0.99 to 16.26 % of planted anthers) and the ability to regenerate plants (from 0 to 3.05 % of planted anthers) in the process of androgenesiss in vitro of winter soft wheat were detected. Fertile regenerants in the anthers culture 5 out of 9 studied hybrids have been obtained. The effectiveness of the last process was different: from 12.5 to 60.0 % (an average of 29.41 %) from the received regenerants. The high degree (8–9 points) of the resistance of the obtained dihaploids to a complex of diseases was shown. Conclusions. Genotype-specific of microspores morphogenetic reactions of soft winter wheat in the process of androgenesis in vitro were revealed. The highest level of callus formation were shown for samples 120/20 and 132/20. 15 dihaploid lines with complex rust resistance and hard smut were obtained.","PeriodicalId":475640,"journal":{"name":"Faktori eksperimentalʹnoï evolûcìï organìzmìv","volume":"88 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135427656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"To the 70th anniversary of the DNA structure discovery","authors":"N. E. Volkova","doi":"10.7124/feeo.v32.1528","DOIUrl":"https://doi.org/10.7124/feeo.v32.1528","url":null,"abstract":"the structure of DNA","PeriodicalId":475640,"journal":{"name":"Faktori eksperimentalʹnoï evolûcìï organìzmìv","volume":"103 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135428129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R. A. Vozhehova, T. Yu. Marchenko, S. V. Chebotar, Yu. O. Lavrynenko, H. G. Bazalii, A. Yu. Zhupina, I. O. Bidnina, V. V. Bazaliy
Aim. To establish correlation-regression models of dependences of grain protein with the duration of the interphase period "flowering- grain maturity" and grain yield in elite numbers in breeding nurseries. Methods. Field research was carried out at the Institute of Irrigated Agriculture of the National Academy of Sciences in 2019-2021. The object of research was modern winter wheat varieties of the Institute; collection samples of the Western European ecotype that were introduced from France and hybrids created with their participation Research methods are field, laboratory, biochemical, selection-genetic, statistical. Results. The nature of the manifestation of the trait "grain protein" in selection numbers selected from hybrid populations with the participation of Western European ecotypes of soft winter wheat, correlation-regression models of the dependence of protein, grain yield and the duration of the "flowering-maturity" period were established. The duration of the period "flowering-ripeness" ranged from 45 to 52 days in the best numbers, and each original hybrid combination had its own optimum, which determined high yield and protein content of the grain. Conclusions. For each hybrid population created with the participation of parental components contrasting in duration of vegetation, it is necessary to develop a specific selection plan taking into account intra-population correlation-regression models of productivity, grain protein content and the duration of the "flowering-maturity" period.
{"title":"Correlation of grain protein content with utilitarian characters in breeding samples of winter wheat, created with the involvement of western european ecotypes","authors":"R. A. Vozhehova, T. Yu. Marchenko, S. V. Chebotar, Yu. O. Lavrynenko, H. G. Bazalii, A. Yu. Zhupina, I. O. Bidnina, V. V. Bazaliy","doi":"10.7124/feeo.v33.1559","DOIUrl":"https://doi.org/10.7124/feeo.v33.1559","url":null,"abstract":"Aim. To establish correlation-regression models of dependences of grain protein with the duration of the interphase period \"flowering- grain maturity\" and grain yield in elite numbers in breeding nurseries. Methods. Field research was carried out at the Institute of Irrigated Agriculture of the National Academy of Sciences in 2019-2021. The object of research was modern winter wheat varieties of the Institute; collection samples of the Western European ecotype that were introduced from France and hybrids created with their participation Research methods are field, laboratory, biochemical, selection-genetic, statistical. Results. The nature of the manifestation of the trait \"grain protein\" in selection numbers selected from hybrid populations with the participation of Western European ecotypes of soft winter wheat, correlation-regression models of the dependence of protein, grain yield and the duration of the \"flowering-maturity\" period were established. The duration of the period \"flowering-ripeness\" ranged from 45 to 52 days in the best numbers, and each original hybrid combination had its own optimum, which determined high yield and protein content of the grain. Conclusions. For each hybrid population created with the participation of parental components contrasting in duration of vegetation, it is necessary to develop a specific selection plan taking into account intra-population correlation-regression models of productivity, grain protein content and the duration of the \"flowering-maturity\" period.","PeriodicalId":475640,"journal":{"name":"Faktori eksperimentalʹnoï evolûcìï organìzmìv","volume":"99 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135428281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. I. Mykhalska, A. G. Komisarenko, V. M. Kurchii, L. I. Bronnikova
Aim. Analysis of physiological and biochemical characteristics of genetically modified wheat with partially suppressed activity of the proline dehydrogenase gene. Methods. Determination of proline dehydrogenase (PDH) activity, free L-proline (Pro); carbohydrate content (sucrose and fructose). Results. It was established that under conditions of water deficit in genetically modified wheat plants with integrated elements forming the double-stranded RNA suppressor of the pdh gene, there is a partial inhibition of the enzyme activity and an increase in the content of free proline. Changes in carbohydrate metabolism under water deficit stress and in the first hours after rehydration were noted, while the sucrose / fructose ratio in control plants significantly decreases during dehydration and normalizes when water supply is restored, in genetically modified plants this indicator almost does not change under short-term water deficit stress. Conclusions. Partial suppression of the proline dehydrogenase gene leads to an increase in the free proline content, the fluctuations of which contribute to the maintenance of carbohydrate balance.
{"title":"Physiological and biochemical characteristics of genetically modified winter wheat","authors":"S. I. Mykhalska, A. G. Komisarenko, V. M. Kurchii, L. I. Bronnikova","doi":"10.7124/feeo.v32.1545","DOIUrl":"https://doi.org/10.7124/feeo.v32.1545","url":null,"abstract":"Aim. Analysis of physiological and biochemical characteristics of genetically modified wheat with partially suppressed activity of the proline dehydrogenase gene. Methods. Determination of proline dehydrogenase (PDH) activity, free L-proline (Pro); carbohydrate content (sucrose and fructose). Results. It was established that under conditions of water deficit in genetically modified wheat plants with integrated elements forming the double-stranded RNA suppressor of the pdh gene, there is a partial inhibition of the enzyme activity and an increase in the content of free proline. Changes in carbohydrate metabolism under water deficit stress and in the first hours after rehydration were noted, while the sucrose / fructose ratio in control plants significantly decreases during dehydration and normalizes when water supply is restored, in genetically modified plants this indicator almost does not change under short-term water deficit stress. Conclusions. Partial suppression of the proline dehydrogenase gene leads to an increase in the free proline content, the fluctuations of which contribute to the maintenance of carbohydrate balance.","PeriodicalId":475640,"journal":{"name":"Faktori eksperimentalʹnoï evolûcìï organìzmìv","volume":"23 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135428294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
А. Yu. Buziashvili, L. О. Biliavska, V. А. Tsygankova, G. O. Iutynska, А. І. Yemets
Aim. Studying of the influence of avermectin-containing polyfunctional preparations Avercom and Avercom Nova on the enhancement of the resistance to fusarium wilt of tomato plants in vitro. Methods. Tomato plants were cultivated in vitro for 30 days in the presence of 25 μl/l of preparations Avercom and Avercom Nova. Analysis of the resistance of tomato plants to fusarium wilt was carried out by spraying with a conidia suspension of Fusarium oxysporum f. sp. lycopersici and F. solani. Resistance to fusarium wilt has been evaluated for 15 days after inoculation with the use of a 9-point scale. Results. It was established that cultivation in vitro of tomato plants cv. Money Maker in the presence of preparations Avercom and Avercom Nova at concentration 25 μl/l enhances the resistance of the selected plants to fusarium wilt caused by F. oxysporum f. sp. lycopersici and F. solani. In particular, resistance to these phytopathogens increased on average from 4 to 6 points on the 10th day after inoculation with F. oxysporum f. sp. lycopersici, and from 2 to 6 points on the 6th day after inoculation with F. solani. Conclusions. The results of the study show the enhancement of the resistance of tomato plants grown in the presence of Avercom and Avercom Nova preparations to the causative agents of the tomato fusarium wilt which indicates the prospects and promising opportunities of the use of microbial biopreparations for the protection of plants against biotic stress.
{"title":"Investigation of the influence of avermectin-containing preparations on the resistance of tomato lines to fusarium blight in vitro","authors":"А. Yu. Buziashvili, L. О. Biliavska, V. А. Tsygankova, G. O. Iutynska, А. І. Yemets","doi":"10.7124/feeo.v32.1539","DOIUrl":"https://doi.org/10.7124/feeo.v32.1539","url":null,"abstract":"Aim. Studying of the influence of avermectin-containing polyfunctional preparations Avercom and Avercom Nova on the enhancement of the resistance to fusarium wilt of tomato plants in vitro. Methods. Tomato plants were cultivated in vitro for 30 days in the presence of 25 μl/l of preparations Avercom and Avercom Nova. Analysis of the resistance of tomato plants to fusarium wilt was carried out by spraying with a conidia suspension of Fusarium oxysporum f. sp. lycopersici and F. solani. Resistance to fusarium wilt has been evaluated for 15 days after inoculation with the use of a 9-point scale. Results. It was established that cultivation in vitro of tomato plants cv. Money Maker in the presence of preparations Avercom and Avercom Nova at concentration 25 μl/l enhances the resistance of the selected plants to fusarium wilt caused by F. oxysporum f. sp. lycopersici and F. solani. In particular, resistance to these phytopathogens increased on average from 4 to 6 points on the 10th day after inoculation with F. oxysporum f. sp. lycopersici, and from 2 to 6 points on the 6th day after inoculation with F. solani. Conclusions. The results of the study show the enhancement of the resistance of tomato plants grown in the presence of Avercom and Avercom Nova preparations to the causative agents of the tomato fusarium wilt which indicates the prospects and promising opportunities of the use of microbial biopreparations for the protection of plants against biotic stress.","PeriodicalId":475640,"journal":{"name":"Faktori eksperimentalʹnoï evolûcìï organìzmìv","volume":"28 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135428640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aim. Production of genetically modified plants of new promising winter wheat genotypes with partial suppression of the proline dehydrogenase gene in in vitro culture and determination of proline content in transgenic and control plants. Methods. Agrobacterium-mediated transformation in culture in vitro, molecular genetic analysis; biochemical determination of proline content; of mathematical statistics. Results. Using the method of Agrobacterium-mediated transformation of callus cultures of new promising genotypes of winter wheat, transgenic plants carrying a double-stranded RNA suppressor of the proline dehydrogenase gene were obtained. The frequency of transformation with the use of the LBA 4404 strain in the studied genotypes was 1.7-2.0 %, and with the use of the AGL0 strain, it was 2.0-2.3 %. It was established that plants with reduced activity of proline dehydrogenase are characterized by a significantly higher content of free L-proline compared to the control. Conclusions. The relatively greater efficiency of using the AGL0 strain for obtaining transgenic plants of winter wheat genotypes with partial suppression of the proline dehydrogenase gene in in vitro culture is shown. The presence of a double-stranded RNA suppressor of the ProDH gene in transgenic plants leads to an increase in the level of free L-proline accumulation.
{"title":"Obtaining genetically modified winter wheat plants with partial suppression of the prolin dehydrogenase gene","authors":"O. V. Dubrovna, L. V. Slyvka, L. G. Velikozhon","doi":"10.7124/feeo.v33.1586","DOIUrl":"https://doi.org/10.7124/feeo.v33.1586","url":null,"abstract":"Aim. Production of genetically modified plants of new promising winter wheat genotypes with partial suppression of the proline dehydrogenase gene in in vitro culture and determination of proline content in transgenic and control plants. Methods. Agrobacterium-mediated transformation in culture in vitro, molecular genetic analysis; biochemical determination of proline content; of mathematical statistics. Results. Using the method of Agrobacterium-mediated transformation of callus cultures of new promising genotypes of winter wheat, transgenic plants carrying a double-stranded RNA suppressor of the proline dehydrogenase gene were obtained. The frequency of transformation with the use of the LBA 4404 strain in the studied genotypes was 1.7-2.0 %, and with the use of the AGL0 strain, it was 2.0-2.3 %. It was established that plants with reduced activity of proline dehydrogenase are characterized by a significantly higher content of free L-proline compared to the control. Conclusions. The relatively greater efficiency of using the AGL0 strain for obtaining transgenic plants of winter wheat genotypes with partial suppression of the proline dehydrogenase gene in in vitro culture is shown. The presence of a double-stranded RNA suppressor of the ProDH gene in transgenic plants leads to an increase in the level of free L-proline accumulation.","PeriodicalId":475640,"journal":{"name":"Faktori eksperimentalʹnoï evolûcìï organìzmìv","volume":"9 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135428626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aim. The purpose of the work is to analyze the data available in the literature on the mechanisms of formation of mycorrhizal symbiosis, in particular, the participation of strigolactones in this process and the influence of mycorrhizal symbiosis on the possibility of plant infection by parasitic plants. Results. The article provides a brief overview of the formation of arbuscular mycorrhizal symbiosis and the role of strigolactones in this process. Evidence is provided that strigolactones secreted by roots into the rhizosphere are signal molecules for AM fungi, as they stimulate their metabolism and induce spore germination, growth and branching of hyphae of AM fungi. In turn, AM fungi release signaling molecules that initiate processes related to the formation of symbiosis in plants. Conclusions. Data available in the literature indicate that strigolactones play an important role in the formation of mycorrhizal symbiosis. Mycorrhizal plants are often less susceptible to infection by parasitic plants, which may depend on the species of both the host plant and the AM fungus. The formation of mycorrhizal symbiosis can be a factor that weakens the infection by parasitic plants, the study of this issue can be important for increasing the effectiveness of the fight against parasitic plants.
{"title":"Formation of arbuscular mycorrhizal symbiosis and its effect on the possibility of infection by parasitic plants","authors":"Zh. Z. Guralchuk, Ye. Yu. Morderer","doi":"10.7124/feeo.v33.1588","DOIUrl":"https://doi.org/10.7124/feeo.v33.1588","url":null,"abstract":"Aim. The purpose of the work is to analyze the data available in the literature on the mechanisms of formation of mycorrhizal symbiosis, in particular, the participation of strigolactones in this process and the influence of mycorrhizal symbiosis on the possibility of plant infection by parasitic plants. Results. The article provides a brief overview of the formation of arbuscular mycorrhizal symbiosis and the role of strigolactones in this process. Evidence is provided that strigolactones secreted by roots into the rhizosphere are signal molecules for AM fungi, as they stimulate their metabolism and induce spore germination, growth and branching of hyphae of AM fungi. In turn, AM fungi release signaling molecules that initiate processes related to the formation of symbiosis in plants. Conclusions. Data available in the literature indicate that strigolactones play an important role in the formation of mycorrhizal symbiosis. Mycorrhizal plants are often less susceptible to infection by parasitic plants, which may depend on the species of both the host plant and the AM fungus. The formation of mycorrhizal symbiosis can be a factor that weakens the infection by parasitic plants, the study of this issue can be important for increasing the effectiveness of the fight against parasitic plants.","PeriodicalId":475640,"journal":{"name":"Faktori eksperimentalʹnoï evolûcìï organìzmìv","volume":"29 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135427658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
O. I. Terpyliak, D. V. Zastavna, K. O. Sosnina, O. L. Filenko
Aim.To study the immunogenetic prerequisites of idiopathic recurrent spontaneous pregnancy losses in humans of allo- and autoimmune genesis. Methods. PCR-SSP (polymerase chain reaction with sequence-specific primers). Results. A comprehensive analysis of the distribution and frequency of allelic variants of the HLA-DRB1, HLA-DQA1, HLA-DQB1 genes testifies to an increased risk of recurrent pregnancy losses with a total homology of 50 % or more in couples and presence of the DQ2.5 genotype in a woman. The OR indicates that the risk of idiopathic recurrent spontaneous pregnancy loss increases 2.68 times when the homology of the couples is 50 % or more for two loci (HLA-DRB1, HLA-DQA1) and 12.8 times when the homology is 50 % or more for by three loci (HLA-DRB1, HLA-DQA1, HLA-DQB1), and if the DQ2.5 genotype is present in women, this risk increases 4 times. Conclusions. HLA genotyping of married couples with a history of spontaneous pregnancy loss is important for preconceptional prevention of recurrent pregnancy losses.
{"title":"The role of the HLA-complex genes in genesis of human idiopathic recurrent pregnancy loss","authors":"O. I. Terpyliak, D. V. Zastavna, K. O. Sosnina, O. L. Filenko","doi":"10.7124/feeo.v33.1573","DOIUrl":"https://doi.org/10.7124/feeo.v33.1573","url":null,"abstract":"Aim.To study the immunogenetic prerequisites of idiopathic recurrent spontaneous pregnancy losses in humans of allo- and autoimmune genesis. Methods. PCR-SSP (polymerase chain reaction with sequence-specific primers). Results. A comprehensive analysis of the distribution and frequency of allelic variants of the HLA-DRB1, HLA-DQA1, HLA-DQB1 genes testifies to an increased risk of recurrent pregnancy losses with a total homology of 50 % or more in couples and presence of the DQ2.5 genotype in a woman. The OR indicates that the risk of idiopathic recurrent spontaneous pregnancy loss increases 2.68 times when the homology of the couples is 50 % or more for two loci (HLA-DRB1, HLA-DQA1) and 12.8 times when the homology is 50 % or more for by three loci (HLA-DRB1, HLA-DQA1, HLA-DQB1), and if the DQ2.5 genotype is present in women, this risk increases 4 times. Conclusions. HLA genotyping of married couples with a history of spontaneous pregnancy loss is important for preconceptional prevention of recurrent pregnancy losses.","PeriodicalId":475640,"journal":{"name":"Faktori eksperimentalʹnoï evolûcìï organìzmìv","volume":"134 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135428124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. O. Kozub, I. O. Sozinov, H. Ya. Bidnyk, N. A. Demianova, O. I. Sozinova, Y. B. Blume
Aim. The aim of the study was to analyze the diversity and frequencies of alleles at the minor gliadin locus Gli-A3 in groups of common winter wheat cultivars developed in different breeding institutions of Ukraine in different periods of time. Methods. Acid polyacrylamide gel electrophoresis of gliadins was carried out. To identify the Gli-A3 allele of the cultivar Myronivska storichna, segregation in a sample of F2 seeds from crossing with the cultivar Bezosta 1 was analyzed. Results. Alleles at Gli-A3 were identified in 511 Ukrainian cultivars. In addition to the alleles a-d, a new allele Gli-A3e encoding two omega-gliadins was identified in Myronivska storichna. The alleles a and b predominate in the total group of cultivars. Differences in the allele composition and frequencies were revealed between some groups of cultivars. A significant reduction in the frequency of Gli-A3b was detected for the groups of the Plant Breeding and Genetics Institute (PBGI) and the V. Ya. Yuryev Plant Production Institute (IPP) cultivars released after 2010. Conclusions. The frequencies of the predominant alleles a and b are similar in the groups of the Central Forest-Steppe cultivars in different periods of breeding. An increase in the frequency of the allele a was noted for the groups of PBGI (the Steppe) and IPP (the Eastern Forest-Steppe) cultivars, which may indicate its adaptive value under conditions of an increase in the average annual temperature in these zones.
{"title":"Alleles at the Gli-A3 locus in groups of ukrainian winter common wheat cultivars of different origin","authors":"N. O. Kozub, I. O. Sozinov, H. Ya. Bidnyk, N. A. Demianova, O. I. Sozinova, Y. B. Blume","doi":"10.7124/feeo.v33.1561","DOIUrl":"https://doi.org/10.7124/feeo.v33.1561","url":null,"abstract":"Aim. The aim of the study was to analyze the diversity and frequencies of alleles at the minor gliadin locus Gli-A3 in groups of common winter wheat cultivars developed in different breeding institutions of Ukraine in different periods of time. Methods. Acid polyacrylamide gel electrophoresis of gliadins was carried out. To identify the Gli-A3 allele of the cultivar Myronivska storichna, segregation in a sample of F2 seeds from crossing with the cultivar Bezosta 1 was analyzed. Results. Alleles at Gli-A3 were identified in 511 Ukrainian cultivars. In addition to the alleles a-d, a new allele Gli-A3e encoding two omega-gliadins was identified in Myronivska storichna. The alleles a and b predominate in the total group of cultivars. Differences in the allele composition and frequencies were revealed between some groups of cultivars. A significant reduction in the frequency of Gli-A3b was detected for the groups of the Plant Breeding and Genetics Institute (PBGI) and the V. Ya. Yuryev Plant Production Institute (IPP) cultivars released after 2010. Conclusions. The frequencies of the predominant alleles a and b are similar in the groups of the Central Forest-Steppe cultivars in different periods of breeding. An increase in the frequency of the allele a was noted for the groups of PBGI (the Steppe) and IPP (the Eastern Forest-Steppe) cultivars, which may indicate its adaptive value under conditions of an increase in the average annual temperature in these zones.","PeriodicalId":475640,"journal":{"name":"Faktori eksperimentalʹnoï evolûcìï organìzmìv","volume":"42 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135428299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aim. The effect of chronic ultraviolet B (UV-B) radiation and cytokinin benzylaminopurine (6-BAP) on the growth, content of photosynthetic pigments and hydrogen peroxide (HP) in leaves of pea plants (Pisum sativum L.) was studied. Methods. Pea plants cultivar Gotivsky were irradiated by chronic UV-B at a dose of 3.5 kJ/m2 per day during 5 days. Groups of the irradiated and non-irradiated plants were treated with 6-BAP after the end of the UV-B radiation. Length of shoots and roots of pea plants were measured during all time of experiment. Content of photosynthetic pigments and HP in leaves were determined during the experiment. Results. It was shown that plant growth delayed, content of photosynthetic pigments reduced, content of HP increased in the leaves after the chronic UV-B radiation of pea plants. The content of pigments increased and content of HP stabilized in pea leaves after the treatment of plants with 6-BAP. Conclusions. It was shown that chronic UV-B radiation of pea plants by dose of 3.5 kJ/m2 per day caused inhibition of plants growth, increasing HP content, decreasing photosynthetic pigments content in mature leaves. The BAP treatment of irradiated plants stimulated the restoration of photosynthetic pigments content in leaves.
{"title":"The response of pea plants to ultraviolet B radiation and cytokinin","authors":"V. V. Zhuk, A. N. Mikheev, L. G. Ovsyannikova","doi":"10.7124/feeo.v32.1541","DOIUrl":"https://doi.org/10.7124/feeo.v32.1541","url":null,"abstract":"Aim. The effect of chronic ultraviolet B (UV-B) radiation and cytokinin benzylaminopurine (6-BAP) on the growth, content of photosynthetic pigments and hydrogen peroxide (HP) in leaves of pea plants (Pisum sativum L.) was studied. Methods. Pea plants cultivar Gotivsky were irradiated by chronic UV-B at a dose of 3.5 kJ/m2 per day during 5 days. Groups of the irradiated and non-irradiated plants were treated with 6-BAP after the end of the UV-B radiation. Length of shoots and roots of pea plants were measured during all time of experiment. Content of photosynthetic pigments and HP in leaves were determined during the experiment. Results. It was shown that plant growth delayed, content of photosynthetic pigments reduced, content of HP increased in the leaves after the chronic UV-B radiation of pea plants. The content of pigments increased and content of HP stabilized in pea leaves after the treatment of plants with 6-BAP. Conclusions. It was shown that chronic UV-B radiation of pea plants by dose of 3.5 kJ/m2 per day caused inhibition of plants growth, increasing HP content, decreasing photosynthetic pigments content in mature leaves. The BAP treatment of irradiated plants stimulated the restoration of photosynthetic pigments content in leaves.","PeriodicalId":475640,"journal":{"name":"Faktori eksperimentalʹnoï evolûcìï organìzmìv","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135428122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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