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Chemical Stability and Bond Strength of Endodontic Sealers Exposed to Acidic, Alkaline and Protein-Rich Environments: An In Vitro Investigation 牙髓密封剂在酸性、碱性和富含蛋白质环境中的化学稳定性和结合强度:一项体外研究。
IF 1.5 4区 医学 Q3 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2026-04-05 Epub Date: 2025-11-22 DOI: 10.1111/aej.70038
Walter Raucci-Neto, Ronaldo Artacho Venter, Antônio Secco Martorano, Victória Gabriela Louzada, Carlos Eduardo Saraiva Miranda, Elias Daniel Covas Rodrigues, Larissa Moreira Spinola de Castro-Raucci

The sealing performance of endodontic sealers may be influenced by environmental factors such as pH and blood-derived proteins. This in vitro study evaluated the effects of pH variation and protein contamination on calcium ion (Ca2+) release and push-out bond strength (BS) of a calcium silicate-based sealer (Bio-C Sealer) and an epoxy resin-based sealer (AH Plus). Standardised bovine root dentine discs were immersed for 28 days in phosphate-buffered saline (PBS) at pH 5, 7 or 12, with or without 10% fetal bovine serum (FBS). Bio-C Sealer showed greater Ca2+ release and conductivity than AH Plus, with enhanced ion release in acidic and reduced in alkaline pH. Both sealers exhibited decreased BS in non-neutral pH, though Bio-C Sealer was more affected by FBS. These findings suggest that pH and protein exposure significantly alter sealer performance, with bioceramic materials being more sensitive to environmental changes than epoxy resin-based sealers.

根管密封剂的密封性能可能受到环境因素的影响,如pH值和血源性蛋白。本体外研究评估了pH变化和蛋白质污染对钙硅酸盐基密封剂(Bio-C sealer)和环氧树脂基密封剂(AH Plus)的钙离子(Ca2+)释放和推出结合强度(BS)的影响。标准牛根牙本质盘在pH值为5、7或12的磷酸盐缓冲盐水(PBS)中浸泡28天,含或不含10%胎牛血清(FBS)。与AH Plus相比,Bio-C Sealer的Ca2+释放和电导率更高,在酸性条件下离子释放增强,在碱性条件下离子释放降低。两种封口剂在非中性pH下均表现出BS降低,但Bio-C Sealer受FBS的影响更大。这些发现表明,pH值和蛋白质暴露会显著改变密封剂的性能,生物陶瓷材料比环氧树脂基密封剂对环境变化更敏感。
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引用次数: 0
Effect of Different Drying Protocols on the Bond Strength of a Bioceramic Root Canal Sealer 不同干燥方式对生物陶瓷根管密封器粘结强度的影响。
IF 1.5 4区 医学 Q3 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2026-04-05 Epub Date: 2025-11-02 DOI: 10.1111/aej.70032
Gizem Kadı, Esin Özlek, Yousef M. K. Saed

This study aimed to investigate how varying levels of dentine moisture affect the push-out bond strength of a bioceramic root canal sealer. Forty-eight root canals were randomly divided into four groups according to drying protocol. Moist group: canals were dried until the last paper point appeared dry, Dry group: 95% ethanol was applied for 10 s, Half-dry group: canals were dried with a single paper point for 5 s, Wet group: canals were left completely flooded. All root canals were obturated with iRoot SP. Root slices were prepared from each sample for push-out testing. The data were statistically analysed using the Kruskal-Wallis and Mann-Whitney U test for pairwise comparisons at a significance level of p < 0.05. The dry group had significantly higher bond strength compared to the wet and half-dry groups (p < 0.05), although no statistically significant difference was observed between the moist and dry groups (p > 0.05).

本研究旨在探讨不同程度的牙本质水分如何影响生物陶瓷根管密封器的推出结合强度。48根管按干燥方案随机分为4组。湿组:干燥至最后一个纸点干燥,干组:95%乙醇干燥10 s,半干组:单纸点干燥5 s,湿组:管道完全淹没。用iRoot SP封闭所有根管。从每个样本中制备根片用于推出测试。数据采用Kruskal-Wallis和Mann-Whitney U检验进行统计学分析,两两比较的显著性水平为p 0.05)。
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引用次数: 0
Micro-CT Evaluation of the Shaping Outcomes of Different Instruments in Oval-Shaped Maxillary Premolar Canals 不同器械在椭圆形上颌前磨牙管内整形效果的微ct评价。
IF 1.5 4区 医学 Q3 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2026-04-05 Epub Date: 2025-11-03 DOI: 10.1111/aej.70028
Merve Yeniçeri Özata, Seda Falakaloğlu, Ali Keleş, Özkan Adıgüzel, Sadullah Kaya

This study evaluated four file systems (WaveOne Gold, Reciproc Blue, ProTaper Next and TruNatomy) for their shaping efficacy in oval-shaped maxillary premolar root canals using micro-CT. Twenty-four extracted premolars, each with two roots, were randomly divided into four groups (n = 6 teeth/group) and instrumented following manufacturer guidelines. Pre- and post-instrumentation micro-CT scans were performed. Key parameters analysed included unprepared canal surface area (% Un-PrepCA), changes in canal area and volume (ΔCA, ΔCV and their percentages), canal transportation and centring ability. Statistical significance was set at p < 0.05. While no significant differences were found in transportation, centring ability or most 3D parameters (ΔCA, ΔCV and ΔSMI), the TRN group exhibited a significantly higher % Un-PrepCA. Conversely, the WOG group achieved a significantly greater % ΔCA compared to TRN and PTN. All systems performed comparably without procedural errors; TRN resulted in the least canal wall contact, while WOG provided the largest prepared canal area.

本研究利用微ct评价了WaveOne Gold、Reciproc Blue、ProTaper Next和TruNatomy四种文件系统在椭圆形上颌前磨牙根管整形中的效果。24颗拔除的前磨牙,每颗有两个牙根,随机分为4组(n = 6颗/组),并按照制造商指南进行矫治。进行仪器前和仪器后的微ct扫描。分析的关键参数包括未准备运河表面积(% Un-PrepCA)、运河面积和体积变化(ΔCA、ΔCV及其百分比)、运河运输和集中能力。统计学意义设为p
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引用次数: 0
Evaluation of Histological Response of Human Pulp to MTA as DPC Agent With LLLT-Split Mouth Randomised Controlled Trial 用lllt -裂口随机对照试验评价MTA作为DPC剂对人牙髓的组织学反应。
IF 1.5 4区 医学 Q3 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2026-04-05 Epub Date: 2025-09-26 DOI: 10.1111/aej.70023
M. Shanmuga Priya, Darshan Dakshindas, Manjusha Warhandpande, Akshay Dhobley, Suchitra Gosavi, Aditi Wasule, Dipak Ghatage

This study examined the histological response of human dental pulp to Mineral Trioxide Aggregate (MTA) as a pulp-capping agent, with or without Low Level Laser Therapy (LLLT). Thirty-two premolars from 16 patients were treated in a split-mouth design: one side received MTA alone, the other MTA with diode laser-assisted haemostasis and decontamination. After 6 weeks, the teeth were extracted for histological analysis. Results showed that the group with LLLT had a higher incidence of complete dentine bridges (87.5% vs. 68.8%) and thicker bridges (> 0.25 mm: 81.3% vs. 68.8%), though differences were not statistically significant. Inflammation was significantly lower in the LLLT group, with all samples showing moderate inflammation compared to 68.8% in controls, and some severe inflammation cases. Tissue organisation was similar between groups. The study suggests that adding LLLT to MTA reduces pulpal inflammation and promotes more uniform, thicker dentine bridges, indicating improved pulp healing.

本研究检测了人类牙髓在低水平激光治疗(LLLT)下对三氧化矿物骨料(MTA)作为牙髓覆盖剂的组织学反应。16例患者的32颗前磨牙采用裂口设计:一侧单独接受MTA治疗,另一侧MTA配合二极管激光辅助止血和去污。6周后拔牙进行组织学分析。结果显示,LLLT组牙本质全桥发生率较高(87.5% vs. 68.8%),牙桥较厚(> 0.25 mm: 81.3% vs. 68.8%),但差异无统计学意义。与对照组的68.8%相比,LLLT组的炎症反应明显较低,所有样本均显示中度炎症,还有一些严重炎症病例。两组间组织结构相似。研究表明,在MTA中加入LLLT可以减少牙髓炎症,促进更均匀、更厚的牙本质桥,表明牙髓愈合得到改善。
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引用次数: 0
Effect of Intracanal Medications on the Viability of Human Periodontal Ligament-Derived Mesenchymal Stem Cells 管内药物对人牙周韧带源性间充质干细胞活力的影响。
IF 1.5 4区 医学 Q3 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2026-04-05 Epub Date: 2025-09-16 DOI: 10.1111/aej.70020
Arian Braido, Walbert de Andrade Vieira, Bruno Cazotti Pereira, Karina Gonzales Silvério, Paulo Henrique Gabriel, Aline Cristine Gomes Matta, Emerson Alves Martins, Adriana de Jesus Soares

This in vitro study evaluated the effects of intracanal medications on the metabolic activity and adhesion of human periodontal ligament-derived mesenchymal stem cells. Tested groups included Control (without medication), Triple Antibiotic Paste (TAP), Double Antibiotic Paste (DAP), calcium hydroxide with saline, calcium hydroxide with 2% chlorhexidine gel and Bio-C Temp. After conditioning, roots were stored in phosphate-buffered saline at 37°C for 21 days. Medications were then removed using 17% EDTA and saline, and dentine samples were used as substrates for cell culture at 3, 5 and 7 days. Metabolic activity was assessed via MTT assay, and adhesion via scanning electron microscopy. None of the medications negatively affected cell viability or adhesion compared to control. TAP showed higher metabolic activity than calcium hydroxide with saline at Day 7. DAP induced earlier cell morphology changes. Bio-C Temp and chlorhexidine gel showed no significant differences at Day 7, confirming overall biocompatibility.

本体外研究评估了管内药物对人牙周韧带源性间充质干细胞代谢活性和粘附的影响。实验组包括对照组(不用药组)、三抗生素膏(TAP)、双抗生素膏(DAP)、氢氧化钙加生理盐水组、氢氧化钙加2%氯己定凝胶组和Bio-C Temp组,调理后将根置于37℃磷酸盐缓冲盐水中保存21 d。然后用17% EDTA和生理盐水去除药物,并将牙本质样本作为底物在3,5和7天进行细胞培养。MTT法测定代谢活性,扫描电镜测定粘附力。与对照组相比,没有一种药物对细胞活力或粘附产生负面影响。在第7天,TAP的代谢活性高于氢氧化钙加生理盐水。DAP诱导早期细胞形态改变。生物温度和氯己定凝胶在第7天无显著差异,证实了整体生物相容性。
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引用次数: 0
Laboratory Evaluation of the Physicochemical Properties of Novel Pre-Mixed Putty-Type Bioceramic Materials 新型预混合腻子型生物陶瓷材料理化性能的实验室评价。
IF 1.5 4区 医学 Q3 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2026-04-05 Epub Date: 2025-10-29 DOI: 10.1111/aej.70031
So-Mang Lee, Minju Song, Sang Won Kwak, Hyeon-Cheol Kim

This study aimed to compare the physicochemical properties of EndoSequence Root Repair Material (ERRM) Fast and CeraPutty with ProRoot MTA and Biodentine, focusing on setting time, radiopacity, microhardness, and pH. Setting time was measured using a Gilmore-type indenter applied every 5 min until no indentation was observed (n = 15 per group). Radiopacity was assessed via aluminium step wedge, microhardness using Vickers hardness, and pH changes with a digital pH meter. Nonparametric statistical analyses were conducted with a significance level of 95%. Biodentine exhibited the shortest setting time and highest microhardness, while ERRM Fast showed the highest radiopacity. High alkaline pH values were measured for all materials. Within the limitations of this study, the new pre-mixed calcium silicate materials exhibited physicochemical properties comparable to traditionally mixed materials, indicating their potential suitability for endodontic treatments. However, careful selection based on clinical requirements, including handling, setting time, and mechanical properties, is essential.

本研究旨在比较EndoSequence根修复材料(ERRM) Fast和CeraPutty与prooroot MTA和Biodentine的理化性质,重点关注凝固时间、透光度、显微硬度和ph。使用gilmore型压头测量凝固时间,每5分钟使用一次,直到没有观察到压痕(每组n = 15)。通过铝阶梯楔评估放射不透明度,显微硬度采用维氏硬度,pH值变化采用数字pH计。进行非参数统计分析,显著性水平为95%。生物牙定凝固时间最短,显微硬度最高,ERRM Fast放射透明度最高。所有材料均测得高碱性pH值。在本研究的限制下,新的预混合硅酸钙材料表现出与传统混合材料相当的物理化学性质,表明它们在牙髓治疗方面的潜在适用性。然而,根据临床要求,包括处理、凝固时间和机械性能,仔细选择是必不可少的。
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引用次数: 0
Clinical Relevance of Large Language Models in Endodontics: Diagnostic Appropriateness Based on 50 Simulated Case Scenarios 牙髓学中大型语言模型的临床相关性:基于50个模拟病例情景的诊断适当性。
IF 1.5 4区 医学 Q3 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2026-04-05 Epub Date: 2025-11-21 DOI: 10.1111/aej.70040
Büşra Karaca, Yunus Emre Çakmak, Damla Erkal

Large language models (LLMs) are increasingly used in healthcare, but their performance in endodontic decision-making remains unclear. This study aimed to compare six LLMs in terms of diagnostic appropriateness for endodontic treatment planning. Fifty clinical scenarios were developed and entered into six LLMs (ChatGPT-4o, ChatGPT-3.5, Claude 4, Copilot, DeepSeek-V3, Gemini 2.5). Two specialists scored responses as appropriate or inappropriate. Repeated measures ANOVA and chi-square tests were used for analysis. Claude showed the highest accuracy (76%), followed by DeepSeek and Gemini. ChatGPT-3.5 had the lowest (40%). Significant differences were found between models (p < 0.05). Performance was better on straightforward cases than on complex scenarios. LLMs vary widely in diagnostic accuracy for endodontic cases. While some models show promise, others may provide confidently incorrect recommendations. Caution and human oversight remain essential until domain-specific, fine-tuned models are developed.

大型语言模型(llm)越来越多地用于医疗保健,但它们在牙髓决策中的表现尚不清楚。本研究旨在比较六种llm在牙髓治疗计划诊断的适宜性。开发了50个临床场景,并进入6个llm (chatgpt - 40, ChatGPT-3.5, Claude 4, Copilot, DeepSeek-V3, Gemini 2.5)。两位专家对回答进行了适当或不适当的评分。采用重复测量方差分析和卡方检验进行分析。Claude的准确率最高(76%),其次是DeepSeek和Gemini。ChatGPT-3.5最低(40%)。模型间差异有统计学意义(p
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引用次数: 0
Effects of Access Cavity Design on the Shaping Ability and Dentine Thickness Following Canal Preparation Using XP-Endo Shaper or Reciproc XP-Endo塑形器和Reciproc塑形器对根管预备后牙本质厚度和塑形能力的影响
IF 1.5 4区 医学 Q3 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2026-04-05 Epub Date: 2025-12-05 DOI: 10.1111/aej.70045
Renata Muniz Alvez Cruz, Ana Flávia Almeida Barbosa, Carolina Oliveira de Lima, Ricardo Tadeu Lopes, Marco Aurélio Versiani, Emmanuel João Nogueira Leal da Silva, Luciana Moura Sassone

This study compared the shaping ability and remaining dentine thickness in mandibular molars prepared with traditional (TradAC) or ultraconservative (UltraAC) access cavities using Reciproc or XP-endo Shaper instruments. Thirty-two molars were scanned with micro-CT before and after preparation to assess canal surface, volume, untouched walls, and dentine thickness. Both access cavity designs showed a significant reduction in dentine thickness after preparation, but no differences were found between TradAC and UltraAC in either mesial or distal roots (p > 0.05). In the distal root, Reciproc produced a greater reduction in dentine thickness compared with XP-endo Shaper (p < 0.05), whereas shaping ability parameters did not differ significantly between instruments (p > 0.05). Some canals exhibited residual dentine thickness below 0.5 mm, particularly when preoperative thickness was already minimal. Within the limitations of this laboratory study, access cavity design had no influence on dentine preservation, while the choice of instrument affected dentine reduction only in the distal root.

本研究比较了在传统(TradAC)和超保守(UltraAC)通道腔中使用Reciproc和XP-endo Shaper器械制备的下颌磨牙的成形能力和剩余牙本质厚度。在预备前后用micro-CT扫描32颗磨牙,评估根管表面、体积、未触及管壁和牙本质厚度。两种通道腔设计均显示制备后牙本质厚度显著降低,但TradAC和UltraAC在近端根和远端根上均无差异(p < 0.05)。在远端牙根,与XP-endo Shaper相比,Reciproc对牙本质厚度的减少更大(p 0.05)。一些根管显示残留牙本质厚度小于0.5 mm,特别是术前厚度已经很小的情况下。在本实验室研究的限制范围内,通道腔的设计对牙本质保存没有影响,而器械的选择仅影响远端牙根的牙本质还原。
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引用次数: 0
Finite Element Analysis of Endodontically Treated Mandibular Second Molars With Variable Root Morphologies: Endocrown vs. Post-And-Core Crown Restorations 根管治疗下颌第二磨牙不同根形态的有限元分析:内冠与桩核冠修复。
IF 1.5 4区 医学 Q3 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2026-04-05 Epub Date: 2025-09-10 DOI: 10.1111/aej.70019
Shenghao Qiu, Ziteng Long, Min Chen, Weiwei Wu, Jingzhi Ma, Yuan Gao, Lin Peng

This study aimed to investigate the biomechanical impact of root canal anatomical variations and restoration techniques on endodontically treated mandibular second molars using finite element analysis. Five root morphologies were modelled: separated-rooted (S), fused-rooted with V-shaped (F-V), U-shaped (F-U) or Ω-shaped (F-Ω) radicular grooves and single-canal fused-rooted (F-O). Micro-CT scans were performed before and after endodontic instrumentation to generate the finite element models: intact teeth, post-and-core crowns with 2- to 3-mm ferrules and endocrowns with 3- to 4-mm pulp chamber extensions. Under axial/oblique loading, von Mises stress distribution was evaluated. Results showed endocrowns had lower peak stresses than post-and-core crowns in common anatomical variations (S, F-U, F-Ω); F-V showed comparable peak stresses; post-and-core crowns with sufficient ferrule height minimised oblique-load stresses in single-canal teeth (F-O). Pronounced stress concentrations were observed at Ω-shaped groove in post-and-core restored teeth. Comprehensive evaluation of root morphology is critical for optimal restoration planning.

本研究旨在通过有限元分析探讨根管解剖变异和修复技术对根管治疗后下颌第二磨牙的生物力学影响。模拟了五种根形态:分离根(S)、融合根与v形(F- v)、u形(F- u)或Ω-shaped (F-Ω)根槽和单管融合根(F- o)。在根管固定前后进行Micro-CT扫描以生成有限元模型:完整的牙齿,2至3毫米卡箍的桩核冠和3至4毫米牙髓腔延伸的根管冠。在轴向/斜向加载下,评估von Mises应力分布。结果表明,在常见的解剖变异中,内冠的峰值应力低于桩核冠(S, F- u, F-Ω);F-V表现出相似的峰值应力;桩核冠有足够的卡圈高度,可以减少单根牙(F-O)的斜负荷应力。柱核修复牙Ω-shaped槽处可见明显的应力集中。根系形态的综合评价是制定最佳修复计划的关键。
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引用次数: 0
Biomechanical Behaviour of Simulated External Cervical Resorption Lesions With Varying 3D Extent Before and After Restoration: Finite Element Analysis Study 不同三维范围模拟颈椎外吸收病变修复前后的生物力学行为:有限元分析研究。
IF 1.5 4区 医学 Q3 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2026-04-05 Epub Date: 2025-09-02 DOI: 10.1111/aej.70017
Mohit Machiwal, Ruchika Roongta Nawal, Sudha Yadav, Sangeeta Talwar, Seema Yadav

This study investigated the biomechanical behaviour of simulated external cervical resorption (ECR) lesions of varying 3D extent, before and after restoration, using finite element analysis (FEA). Seven maxillary incisors were modelled, representing Patel's classification stages: Group 1–2Bp, 2–2Cp, 3–2Cd, 4–3 Bd, 5–3Cd, 6–3Bp and 7–Control. ECR lesions were restored using Biodentine, with endodontic treatment when the pulp was involved. CBCT scans were used to generate 3D models, and a 240 N bite force was applied to evaluate stress via Von Mises and principal stress values. Pre-treatment, all ECR lesions showed concentrated stress, highest in Group 3Bp (538.11 MPa), correlating directly with lesion height, circumferential spread and pulp proximity. Post-treatment, biodentine reduced stress and improved the safety factor (SF), although most groups still had a failure index > 1, indicating residual fracture risk. Pulp involvement had the greatest biomechanical impact, highlighting the importance of early detection and classification-based treatment planning.

本研究利用有限元分析(FEA)研究了不同三维程度的模拟颈椎外吸收(ECR)病变在修复前后的生物力学行为。7个上颌切牙模型,分别代表Patel分类阶段:1-2Bp组、2-2Cp组、3-2Cd组、4-3 Bd组、5-3Cd组、6-3Bp组和7-Control组。当牙髓受损时,采用根管治疗,使用百牙妥定修复ECR病变。采用CBCT扫描生成三维模型,并施加240 N咬合力,通过Von Mises和主应力值评估应力。治疗前,所有ECR病变均表现为应力集中,3Bp组应力最高(538.11 MPa),与病变高度、周向扩散和牙髓接近度直接相关。治疗后,生物牙汀降低了应力,提高了安全系数(SF),尽管大多数组仍有失败指数>.1,表明存在残余骨折风险。牙髓受累具有最大的生物力学影响,突出了早期发现和基于分类的治疗计划的重要性。
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引用次数: 0
期刊
Australian Endodontic Journal
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