Pub Date : 2026-04-05Epub Date: 2025-11-22DOI: 10.1111/aej.70038
Walter Raucci-Neto, Ronaldo Artacho Venter, Antônio Secco Martorano, Victória Gabriela Louzada, Carlos Eduardo Saraiva Miranda, Elias Daniel Covas Rodrigues, Larissa Moreira Spinola de Castro-Raucci
The sealing performance of endodontic sealers may be influenced by environmental factors such as pH and blood-derived proteins. This in vitro study evaluated the effects of pH variation and protein contamination on calcium ion (Ca2+) release and push-out bond strength (BS) of a calcium silicate-based sealer (Bio-C Sealer) and an epoxy resin-based sealer (AH Plus). Standardised bovine root dentine discs were immersed for 28 days in phosphate-buffered saline (PBS) at pH 5, 7 or 12, with or without 10% fetal bovine serum (FBS). Bio-C Sealer showed greater Ca2+ release and conductivity than AH Plus, with enhanced ion release in acidic and reduced in alkaline pH. Both sealers exhibited decreased BS in non-neutral pH, though Bio-C Sealer was more affected by FBS. These findings suggest that pH and protein exposure significantly alter sealer performance, with bioceramic materials being more sensitive to environmental changes than epoxy resin-based sealers.
{"title":"Chemical Stability and Bond Strength of Endodontic Sealers Exposed to Acidic, Alkaline and Protein-Rich Environments: An In Vitro Investigation","authors":"Walter Raucci-Neto, Ronaldo Artacho Venter, Antônio Secco Martorano, Victória Gabriela Louzada, Carlos Eduardo Saraiva Miranda, Elias Daniel Covas Rodrigues, Larissa Moreira Spinola de Castro-Raucci","doi":"10.1111/aej.70038","DOIUrl":"10.1111/aej.70038","url":null,"abstract":"<p>The sealing performance of endodontic sealers may be influenced by environmental factors such as pH and blood-derived proteins. This in vitro study evaluated the effects of pH variation and protein contamination on calcium ion (Ca<sup>2+</sup>) release and push-out bond strength (BS) of a calcium silicate-based sealer (Bio-C Sealer) and an epoxy resin-based sealer (AH Plus). Standardised bovine root dentine discs were immersed for 28 days in phosphate-buffered saline (PBS) at pH 5, 7 or 12, with or without 10% fetal bovine serum (FBS). Bio-C Sealer showed greater Ca<sup>2+</sup> release and conductivity than AH Plus, with enhanced ion release in acidic and reduced in alkaline pH. Both sealers exhibited decreased BS in non-neutral pH, though Bio-C Sealer was more affected by FBS. These findings suggest that pH and protein exposure significantly alter sealer performance, with bioceramic materials being more sensitive to environmental changes than epoxy resin-based sealers.</p>","PeriodicalId":55581,"journal":{"name":"Australian Endodontic Journal","volume":"52 1","pages":"139-147"},"PeriodicalIF":1.5,"publicationDate":"2026-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13051046/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145575010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}