首页 > 最新文献
paperpass

La Chirurgia degli organi di movimento最新文献

英文 中文
Anterolateral soft tissue sparing approach to the hip. Surgical technique. 保留髋关节前外侧软组织入路。手术技术。
Pub Date : 2008-05-01 Epub Date: 2008-04-12 DOI: 10.1007/s12306-008-0040-9
Aldo Toni, Francesco Traina, Marcello De Fine, Lorenzo Garagnani, Armando Cervini, Enrico Tassinari

The long-term successful outcomes of total hip replacement led to an extension of the surgical indications to young and active patients. To achieve a prompt functional recovery, varying surgical techniques have been proposed. In this study we present our anterolateral soft tissue sparing approach characterised by a lesser dissection of the muscles, a capsular repair and an accurate soft tissue tensioning and reconstruction. The target of this procedure is a full and quick restoration of hip function, reducing the rate of early complications such as hip dislocation.

全髋关节置换术的长期成功结果导致手术指征扩展到年轻和活跃的患者。为了实现功能的迅速恢复,不同的手术技术已被提出。在这项研究中,我们提出了我们的前外侧软组织保留入路,其特点是较少的肌肉夹层,包膜修复和准确的软组织张紧和重建。该手术的目标是全面快速地恢复髋关节功能,减少早期并发症如髋关节脱位的发生率。
{"title":"Anterolateral soft tissue sparing approach to the hip. Surgical technique.","authors":"Aldo Toni,&nbsp;Francesco Traina,&nbsp;Marcello De Fine,&nbsp;Lorenzo Garagnani,&nbsp;Armando Cervini,&nbsp;Enrico Tassinari","doi":"10.1007/s12306-008-0040-9","DOIUrl":"https://doi.org/10.1007/s12306-008-0040-9","url":null,"abstract":"<p><p>The long-term successful outcomes of total hip replacement led to an extension of the surgical indications to young and active patients. To achieve a prompt functional recovery, varying surgical techniques have been proposed. In this study we present our anterolateral soft tissue sparing approach characterised by a lesser dissection of the muscles, a capsular repair and an accurate soft tissue tensioning and reconstruction. The target of this procedure is a full and quick restoration of hip function, reducing the rate of early complications such as hip dislocation.</p>","PeriodicalId":76085,"journal":{"name":"La Chirurgia degli organi di movimento","volume":"92 1","pages":"23-7"},"PeriodicalIF":0.0,"publicationDate":"2008-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s12306-008-0040-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27379666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 5
Rupture of the tibialis posterior tendon in a closed ankle fracture: a case report. 闭合性踝关节骨折致胫骨后肌腱断裂1例。
Pub Date : 2008-04-01 Epub Date: 2008-05-21 DOI: 10.1007/s12306-007-0028-x
Francesco Ceccarelli, Cesare Faldini, Stavroula Pagkrati, Sandro Giannini

Rupture of the tibialis posterior tendon may occur during a trauma in pronation-external rotation of the foot or, less commonly, during a direct trauma of the ankle. When an isolated fracture of the medial malleolus is present, it is more likely that a direct trauma has occurred. A 36-year-old man with a non-displaced medial malleolar fracture was evaluated. Repair of the tendon and reduction of the fracture were performed. Twenty-four months after the operation, the fracture was completely healed, the patient was asymptomatic, he had a normal ankle range of motion, and the function and strength of the tibialis posterior tendon were equal to those on the contralateral side. Early surgical repair of the tibialis posterior tendon combined with malleolar fracture reduction is recommended to avoid progression to a plano-valgus foot.

胫骨后肌腱断裂可发生在足内旋-外旋创伤中,或较少发生在踝关节直接创伤中。当内踝出现孤立性骨折时,更有可能发生直接创伤。一位36岁男性非移位内踝骨折被评估。进行肌腱修复和骨折复位。术后24个月,骨折完全愈合,患者无症状,踝关节活动范围正常,胫骨后腱功能和强度与对侧相当。建议早期手术修复胫骨后腱并结合踝骨折复位,以避免进展为平外翻足。
{"title":"Rupture of the tibialis posterior tendon in a closed ankle fracture: a case report.","authors":"Francesco Ceccarelli,&nbsp;Cesare Faldini,&nbsp;Stavroula Pagkrati,&nbsp;Sandro Giannini","doi":"10.1007/s12306-007-0028-x","DOIUrl":"https://doi.org/10.1007/s12306-007-0028-x","url":null,"abstract":"<p><p>Rupture of the tibialis posterior tendon may occur during a trauma in pronation-external rotation of the foot or, less commonly, during a direct trauma of the ankle. When an isolated fracture of the medial malleolus is present, it is more likely that a direct trauma has occurred. A 36-year-old man with a non-displaced medial malleolar fracture was evaluated. Repair of the tendon and reduction of the fracture were performed. Twenty-four months after the operation, the fracture was completely healed, the patient was asymptomatic, he had a normal ankle range of motion, and the function and strength of the tibialis posterior tendon were equal to those on the contralateral side. Early surgical repair of the tibialis posterior tendon combined with malleolar fracture reduction is recommended to avoid progression to a plano-valgus foot.</p>","PeriodicalId":76085,"journal":{"name":"La Chirurgia degli organi di movimento","volume":"91 3","pages":"167-70"},"PeriodicalIF":0.0,"publicationDate":"2008-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s12306-007-0028-x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27455333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 21
Methods to avoid gamma nail complications. 方法:避免gamma甲并发症。
Pub Date : 2008-04-01 Epub Date: 2008-05-21 DOI: 10.1007/s12306-007-0030-3
Raffaele Pascarella, Giuseppe Cucca, Alessandra Maresca, Matteo Commessatti, Giovanni Bracci, Stefano Boriani, Enrico Gozzi

Femoral neck fractures are progressively increasing, due to higher survival rates, particularly among the female population. The gamma nail was created to treat intertrochanteric fracture types 31-A1, 31-A2 and 31-A3 and in some cases basicervical fractures of type 31-B2-1. Complications can be classified as intraoperative and postoperative. The intraoperative might be related to the nail's introduction site, lag and distal locking screw positions. Postoperative complications depend mostly on an incorrect surgical technique, which can lead to an inaccurate nail position and consequent implant failure. We rarely observe failures caused by severe bone osteoporosis.

股骨颈骨折正逐渐增加,由于较高的存活率,特别是在女性人群中。gamma钉用于治疗31-A1、31-A2和31-A3型粗隆间骨折以及一些31-B2-1型基础颈椎骨折。并发症可分为术中和术后。术中可能与钉入位置、迟滞和远端锁定螺钉位置有关。术后并发症主要取决于不正确的手术技术,这可能导致不准确的指甲位置和随后的种植失败。我们很少观察到严重骨质疏松引起的失败。
{"title":"Methods to avoid gamma nail complications.","authors":"Raffaele Pascarella,&nbsp;Giuseppe Cucca,&nbsp;Alessandra Maresca,&nbsp;Matteo Commessatti,&nbsp;Giovanni Bracci,&nbsp;Stefano Boriani,&nbsp;Enrico Gozzi","doi":"10.1007/s12306-007-0030-3","DOIUrl":"https://doi.org/10.1007/s12306-007-0030-3","url":null,"abstract":"<p><p>Femoral neck fractures are progressively increasing, due to higher survival rates, particularly among the female population. The gamma nail was created to treat intertrochanteric fracture types 31-A1, 31-A2 and 31-A3 and in some cases basicervical fractures of type 31-B2-1. Complications can be classified as intraoperative and postoperative. The intraoperative might be related to the nail's introduction site, lag and distal locking screw positions. Postoperative complications depend mostly on an incorrect surgical technique, which can lead to an inaccurate nail position and consequent implant failure. We rarely observe failures caused by severe bone osteoporosis.</p>","PeriodicalId":76085,"journal":{"name":"La Chirurgia degli organi di movimento","volume":"91 3","pages":"133-9"},"PeriodicalIF":0.0,"publicationDate":"2008-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s12306-007-0030-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27453267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 14
Pathological dislocation of the hip in neurofibromatosis: a case report. 神经纤维瘤病致髋关节病理性脱位1例。
Pub Date : 2008-04-01 Epub Date: 2008-05-21 DOI: 10.1007/s12306-007-0027-y
Manuele Lampasi, Tiziana Greggi, Alessandra Sudanese

The Authors report the case of a 28-year-old patient with type 1 neurofibromatosis, who presented a pathological dislocation of the hip. This event was preceded by the development of a neurological pattern of spastic paraparesis and decreased pain sensation in both lower limbs, secondary to the progression of a dystrophic kyphoscoliosis. Pathological dislocation of the hip in neurofibromatosis has been reported in the literature as a consequence of intra-articular growth of neurofibromas. Conversely, in the case described here, clinical and radiographic features and the absence of neurofibromas in the joint, verified by means of radiographic and histological examinations, suggested the diagnosis of neuropathic arthropathy. The treatment of hip dislocation should be differentiated according to the aetiology, given the underlying articular instability in cases of neuropathic arthropathy.

作者报告的情况下,28岁的患者1型神经纤维瘤病,谁提出了病理性脱位的髋关节。在此事件发生之前,神经学模式的发展为痉挛性截瘫和双下肢疼痛感觉减少,继发于营养不良性后凸脊柱侧凸的进展。神经纤维瘤病中髋关节病理性脱位的文献报道是神经纤维瘤在关节内生长的结果。相反,在本病例中,临床和影像学特征以及关节内没有神经纤维瘤,通过影像学和组织学检查证实,提示神经性关节病的诊断。考虑到神经性关节病患者潜在的关节不稳定,髋关节脱位的治疗应根据病因进行区分。
{"title":"Pathological dislocation of the hip in neurofibromatosis: a case report.","authors":"Manuele Lampasi,&nbsp;Tiziana Greggi,&nbsp;Alessandra Sudanese","doi":"10.1007/s12306-007-0027-y","DOIUrl":"https://doi.org/10.1007/s12306-007-0027-y","url":null,"abstract":"<p><p>The Authors report the case of a 28-year-old patient with type 1 neurofibromatosis, who presented a pathological dislocation of the hip. This event was preceded by the development of a neurological pattern of spastic paraparesis and decreased pain sensation in both lower limbs, secondary to the progression of a dystrophic kyphoscoliosis. Pathological dislocation of the hip in neurofibromatosis has been reported in the literature as a consequence of intra-articular growth of neurofibromas. Conversely, in the case described here, clinical and radiographic features and the absence of neurofibromas in the joint, verified by means of radiographic and histological examinations, suggested the diagnosis of neuropathic arthropathy. The treatment of hip dislocation should be differentiated according to the aetiology, given the underlying articular instability in cases of neuropathic arthropathy.</p>","PeriodicalId":76085,"journal":{"name":"La Chirurgia degli organi di movimento","volume":"91 3","pages":"163-6"},"PeriodicalIF":0.0,"publicationDate":"2008-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s12306-007-0027-y","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27455332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 5
Histological and immunohistochemical analysis of an allogenic bone graft engineered with autologous bone marrow mononuclear cells in the treatment of a large segmental defect of the ulna. A case report. 自体骨髓单核细胞异体骨移植治疗尺骨大节段缺损的组织学和免疫组织化学分析。一份病例报告。
Pub Date : 2008-04-01 Epub Date: 2008-05-21 DOI: 10.1007/s12306-007-0029-9
Sandro Giannini, Francesca Vannini, Gina Lisignoli, Andrea Facchini

The treatment of large (6 cm) segmental bone defects, especially in areas associated with soft tissue damages and exposure, is challenging. Massive bone allografts are widely used in reconstructive surgery to replace missing bone parts such as critical size defects, nevertheless, early vascular invasion is a critical factor in bone allograft incorporation and in the consequent success of the surgery. Unfortunately, it has been proven that only slow and incomplete bone integration is possible by using frozen allografts in the treatment of large bone defects. An allogenic frozen bone graft was engineered with bone marrow mononuclear cells (BMMCs) to repair a large ulna defect in a male patient. When infection and sinus developed at the graft site 4 months after implantation, the partially reabsorbed graft was removed and send to the lab for examination. The histological and immunohistochemical analysis performed on a graft removed from the central zone of the defect demonstrated areas of neo-vascularisation, indicating that a remodelling process was actively occurring even in an area not usually repopulated with the conventional techniques. For these reasons, we may hypothesise that the use of BMMCs has a role in the re-building of large segmental defects.

大(6厘米)节段性骨缺损的治疗,特别是与软组织损伤和暴露相关的区域,是具有挑战性的。大量同种异体骨移植被广泛应用于重建手术,以取代缺失的骨部分,如临界大小的缺陷,然而,早期血管入侵是同种异体骨植入和手术成功的关键因素。不幸的是,已经证明只有缓慢和不完全的骨整合是可能的,使用冷冻同种异体移植物治疗大骨缺损。用骨髓单核细胞(BMMCs)工程化的同种异体冷冻骨移植修复了一名男性患者的大面积尺骨缺损。植入4个月后,当移植物部位出现感染和鼻窦时,将部分重吸收的移植物取出送实验室检查。对从缺损中心区域移除的移植物进行的组织学和免疫组织化学分析显示了新血管化的区域,这表明即使在传统技术通常无法重建的区域,重建过程也在积极发生。基于这些原因,我们可以假设bmmc的使用在大节段缺损的重建中起作用。
{"title":"Histological and immunohistochemical analysis of an allogenic bone graft engineered with autologous bone marrow mononuclear cells in the treatment of a large segmental defect of the ulna. A case report.","authors":"Sandro Giannini,&nbsp;Francesca Vannini,&nbsp;Gina Lisignoli,&nbsp;Andrea Facchini","doi":"10.1007/s12306-007-0029-9","DOIUrl":"https://doi.org/10.1007/s12306-007-0029-9","url":null,"abstract":"<p><p>The treatment of large (6 cm) segmental bone defects, especially in areas associated with soft tissue damages and exposure, is challenging. Massive bone allografts are widely used in reconstructive surgery to replace missing bone parts such as critical size defects, nevertheless, early vascular invasion is a critical factor in bone allograft incorporation and in the consequent success of the surgery. Unfortunately, it has been proven that only slow and incomplete bone integration is possible by using frozen allografts in the treatment of large bone defects. An allogenic frozen bone graft was engineered with bone marrow mononuclear cells (BMMCs) to repair a large ulna defect in a male patient. When infection and sinus developed at the graft site 4 months after implantation, the partially reabsorbed graft was removed and send to the lab for examination. The histological and immunohistochemical analysis performed on a graft removed from the central zone of the defect demonstrated areas of neo-vascularisation, indicating that a remodelling process was actively occurring even in an area not usually repopulated with the conventional techniques. For these reasons, we may hypothesise that the use of BMMCs has a role in the re-building of large segmental defects.</p>","PeriodicalId":76085,"journal":{"name":"La Chirurgia degli organi di movimento","volume":"91 3","pages":"171-5"},"PeriodicalIF":0.0,"publicationDate":"2008-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s12306-007-0029-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27455334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 10
The use of fluoride cement: preliminary experimental study and clinical application. 氟化水泥的应用:初步实验研究及临床应用。
Pub Date : 2008-04-01 Epub Date: 2008-05-21 DOI: 10.1007/s12306-007-0023-2
Claudia Di Bella, Enrico Lucarelli, Milena Fini, Roberto Giardino, Mario Mercuri, Davide Donati

One of the most common complications of cemented prostheses is aseptic loosening of the implant, which is often due to mechanical breakdown at the bone-cement interface. To improve the characteristics of PMMA, the addition of fluoride has been proposed, which, without jeopardising its mechanical qualities, should provide better integration with the surrounding bone. An experimental study was performed in 4 sheep. A cylinder of cement (with and without fluoride) was inserted in the meta-epiphysis of the distal femur. A clinical study followed 7 patients. The cement with added fluoride was used as a filler after the curettage of a benign tumour located in the meta-epiphysis around the knee. Histological results in animals combined with long-term radiographic results in humans have shown the positive influence of fluoride in the cement, as it allows better integration of the cement with the surrounding bone.

骨水泥假体最常见的并发症之一是植入物的无菌性松动,这通常是由于骨水泥界面的机械破坏。为了改善PMMA的特性,已经提出添加氟化物,这在不损害其机械质量的情况下,应该能更好地与周围的骨骼结合。对4只羊进行了实验研究。在股骨远端骨骺后端插入一柱骨水泥(含氟和不含氟)。一项临床研究随访了7例患者。加入氟化物的水泥被用作膝关节周围骨骺后良性肿瘤刮除后的填充物。动物的组织学结果和人类的长期放射学结果都显示了骨水泥中氟化物的积极影响,因为它可以使骨水泥更好地与周围骨骼结合。
{"title":"The use of fluoride cement: preliminary experimental study and clinical application.","authors":"Claudia Di Bella,&nbsp;Enrico Lucarelli,&nbsp;Milena Fini,&nbsp;Roberto Giardino,&nbsp;Mario Mercuri,&nbsp;Davide Donati","doi":"10.1007/s12306-007-0023-2","DOIUrl":"https://doi.org/10.1007/s12306-007-0023-2","url":null,"abstract":"<p><p>One of the most common complications of cemented prostheses is aseptic loosening of the implant, which is often due to mechanical breakdown at the bone-cement interface. To improve the characteristics of PMMA, the addition of fluoride has been proposed, which, without jeopardising its mechanical qualities, should provide better integration with the surrounding bone. An experimental study was performed in 4 sheep. A cylinder of cement (with and without fluoride) was inserted in the meta-epiphysis of the distal femur. A clinical study followed 7 patients. The cement with added fluoride was used as a filler after the curettage of a benign tumour located in the meta-epiphysis around the knee. Histological results in animals combined with long-term radiographic results in humans have shown the positive influence of fluoride in the cement, as it allows better integration of the cement with the surrounding bone.</p>","PeriodicalId":76085,"journal":{"name":"La Chirurgia degli organi di movimento","volume":"91 3","pages":"141-6"},"PeriodicalIF":0.0,"publicationDate":"2008-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s12306-007-0023-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27455328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Charcot's joint secondary to neurologic complications of epidural anaesthesia: a case report. 硬膜外麻醉继发神经系统并发症Charcot关节一例报告。
Pub Date : 2008-04-01 Epub Date: 2008-05-21 DOI: 10.1007/s12306-007-0026-z
Alessandra Sudanese, Federico Giardina, Federico Biondi, Francesco Traina, Franco Bertoni, Aldo Toni

Neurogenic arthropathy or Charcot's Joint is an articular pathology that can strike patient with neurological effects or can be the result of a neurological lesion with deficit of the superficial and deep sensitivity. In this article we introduce one case Charcot's Joint of the hip, caused by an epidural anaesthesia performed for a surgery of the other hip.

神经源性关节病或夏可氏关节是一种关节病变,可使患者受到神经系统的影响,也可由神经系统损伤引起浅表和深层敏感性的缺陷。在这篇文章中,我们介绍了一例髋关节沙科氏关节,由硬膜外麻醉引起的另一个髋关节的手术。
{"title":"Charcot's joint secondary to neurologic complications of epidural anaesthesia: a case report.","authors":"Alessandra Sudanese, Federico Giardina, Federico Biondi, Francesco Traina, Franco Bertoni, Aldo Toni","doi":"10.1007/s12306-007-0026-z","DOIUrl":"10.1007/s12306-007-0026-z","url":null,"abstract":"<p><p>Neurogenic arthropathy or Charcot's Joint is an articular pathology that can strike patient with neurological effects or can be the result of a neurological lesion with deficit of the superficial and deep sensitivity. In this article we introduce one case Charcot's Joint of the hip, caused by an epidural anaesthesia performed for a surgery of the other hip.</p>","PeriodicalId":76085,"journal":{"name":"La Chirurgia degli organi di movimento","volume":"91 3","pages":"159-62"},"PeriodicalIF":0.0,"publicationDate":"2008-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27455331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cell manipulation in autologous chondrocyte implantation: from research to cleanroom. 自体软骨细胞植入中的细胞操作:从研究到洁净室。
Pub Date : 2008-04-01 Epub Date: 2008-05-21 DOI: 10.1007/s12306-007-0024-1
Livia Roseti, Marta Serra, Domenico Tigani, Irene Brognara, Annamaria Lopriore, Alessandra Bassi, Pier Maria Fornasari

In the field of orthopaedics, autologous chondrocyte implantation is a technique currently used for the regeneration of damaged articular cartilage. There is evidence of the neo-formation of tissue displaying characteristics similar to hyaline cartilage. In vitro chondrocyte manipulation is a crucial phase of this therapeutic treatment consisting of different steps: cell isolation from a cartilage biopsy, expansion in monolayer culture and growth onto a three-dimensional biomaterial to implant in the damaged area. To minimise the risk of in vitro cell contamination, the manipulation must be performed in a controlled environment such as a cleanroom. Moreover, the choice of reagents and raw material suitable for clinical use in humans and the translation of research protocols into standardised production processes are important. In this study we describe the preliminary results obtained by the development of chondrocyte manipulation protocols (isolation and monolayer expansion) in cleanrooms for the application of autologous implantation.

在骨科领域,自体软骨细胞植入是目前用于损伤关节软骨再生的一种技术。有证据表明新形成的组织显示出与透明软骨相似的特征。体外软骨细胞操作是该治疗的关键阶段,包括不同的步骤:从软骨活检中分离细胞,单层培养中扩增,生长到三维生物材料上并植入受损区域。为了尽量减少体外细胞污染的风险,操作必须在受控环境中进行,如洁净室。此外,选择适合人类临床使用的试剂和原料以及将研究方案转化为标准化生产过程也很重要。在这项研究中,我们描述了通过在洁净室中开发用于自体植入的软骨细胞操作方案(分离和单层扩增)获得的初步结果。
{"title":"Cell manipulation in autologous chondrocyte implantation: from research to cleanroom.","authors":"Livia Roseti,&nbsp;Marta Serra,&nbsp;Domenico Tigani,&nbsp;Irene Brognara,&nbsp;Annamaria Lopriore,&nbsp;Alessandra Bassi,&nbsp;Pier Maria Fornasari","doi":"10.1007/s12306-007-0024-1","DOIUrl":"https://doi.org/10.1007/s12306-007-0024-1","url":null,"abstract":"<p><p>In the field of orthopaedics, autologous chondrocyte implantation is a technique currently used for the regeneration of damaged articular cartilage. There is evidence of the neo-formation of tissue displaying characteristics similar to hyaline cartilage. In vitro chondrocyte manipulation is a crucial phase of this therapeutic treatment consisting of different steps: cell isolation from a cartilage biopsy, expansion in monolayer culture and growth onto a three-dimensional biomaterial to implant in the damaged area. To minimise the risk of in vitro cell contamination, the manipulation must be performed in a controlled environment such as a cleanroom. Moreover, the choice of reagents and raw material suitable for clinical use in humans and the translation of research protocols into standardised production processes are important. In this study we describe the preliminary results obtained by the development of chondrocyte manipulation protocols (isolation and monolayer expansion) in cleanrooms for the application of autologous implantation.</p>","PeriodicalId":76085,"journal":{"name":"La Chirurgia degli organi di movimento","volume":"91 3","pages":"147-51"},"PeriodicalIF":0.0,"publicationDate":"2008-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s12306-007-0024-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27455329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 16
Vancomycin and meropenem in acrylic cement: elution kinetics of in vitro bactericidal action. 万古霉素和美罗培南在丙烯酸水泥中的体外杀菌作用的洗脱动力学。
Pub Date : 2008-04-01 Epub Date: 2008-05-21 DOI: 10.1007/s12306-007-0025-0
Agnese Andollina, Giorgia Bertoni, Franco Bertoni, Carola Zolezzi, Federico Trentani, Paolo Trentani, Anna Maria Borrelli, Domenico Tigani

The Authors present a paper with a dual goal: in vitro evaluation of the elution of vancomycin and monitoring of its bactericidal action when the antibiotic is used in acrylic cement. Discs of cement with different concentrations of vancomycin alone or combined with meropenem were prepared. To assess the elution of vancomycin the discs were kept in physiological solution and periodically sampled for five weeks. The bactericidal action was assessed by putting the antibiotic discs in contact with colonies of Staphylococcus, Enterococcus, Pseudomonas and Escherichia coli. Two combinations of antibiotic-loaded cement were tested: the first one to act as a spacer and the second to stabilise the revision prosthesis.

作者提出了一篇具有双重目的的论文:体外评价万古霉素在丙烯酸水泥中的洗脱作用和监测万古霉素在丙烯酸水泥中的杀菌作用。制备了不同浓度万古霉素单用或美罗培南联用的水泥片。为了评估万古霉素的洗脱效果,将圆盘保存在生理溶液中并定期取样5周。将抗生素片与葡萄球菌、肠球菌、假单胞菌和大肠杆菌菌落接触,评价其杀菌作用。测试了两种含抗生素的水泥组合:第一种用作间隔物,第二种用于稳定修复假体。
{"title":"Vancomycin and meropenem in acrylic cement: elution kinetics of in vitro bactericidal action.","authors":"Agnese Andollina,&nbsp;Giorgia Bertoni,&nbsp;Franco Bertoni,&nbsp;Carola Zolezzi,&nbsp;Federico Trentani,&nbsp;Paolo Trentani,&nbsp;Anna Maria Borrelli,&nbsp;Domenico Tigani","doi":"10.1007/s12306-007-0025-0","DOIUrl":"https://doi.org/10.1007/s12306-007-0025-0","url":null,"abstract":"<p><p>The Authors present a paper with a dual goal: in vitro evaluation of the elution of vancomycin and monitoring of its bactericidal action when the antibiotic is used in acrylic cement. Discs of cement with different concentrations of vancomycin alone or combined with meropenem were prepared. To assess the elution of vancomycin the discs were kept in physiological solution and periodically sampled for five weeks. The bactericidal action was assessed by putting the antibiotic discs in contact with colonies of Staphylococcus, Enterococcus, Pseudomonas and Escherichia coli. Two combinations of antibiotic-loaded cement were tested: the first one to act as a spacer and the second to stabilise the revision prosthesis.</p>","PeriodicalId":76085,"journal":{"name":"La Chirurgia degli organi di movimento","volume":"91 3","pages":"153-8"},"PeriodicalIF":0.0,"publicationDate":"2008-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s12306-007-0025-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27455330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 24
The use of pre-contoured plates in the treatment of C2-C3 fractures of the distal humerus: clinical experience. 预成形钢板治疗肱骨远端C2-C3骨折的临床经验
Pub Date : 2008-02-01 Epub Date: 2008-03-03 DOI: 10.1007/s12306-007-0022-3
Andrea Celli, Maria Teresa Donini, Claudio Minervini

Open reduction and internal fixation is the treatment of choice in distal humerus fractures. The aims of the treatment are to obtain anatomic reconstruction and restoration of the elbow's geometry followed by stable internal fixation that allows immediate rehabilitation. The plates are pre-contoured to fit the natural anatomy of the elbow and in the case of complex fractures they provide a guide for the anatomic restoration of the distal humerus. The two plates are placed parallel with the screws locked together by interdigitation creating a fixed angle in the distal fragments that provides stability to the entire distal humerus. Using this method we obtained good results according to the Mayo Elbow Performance Score (MEPS) and we are able to reduce the incidence of complications. Our experience shows also that it can be useful to obtain an anatomical reduction of distal humeral fractures using the pre-contoured plates in a parallel position recovering the anatomical antiversion of the articular surface.

切开复位内固定是肱骨远端骨折的首选治疗方法。治疗的目的是获得解剖重建和肘关节几何形状的恢复,然后进行稳定的内固定,以便立即康复。钢板是预先成形的,以适应肘关节的自然解剖结构,在复杂骨折的情况下,它们为肱骨远端解剖恢复提供指导。两个钢板平行放置,螺钉通过指间锁在一起,在远端碎片中形成固定角度,为整个肱骨远端提供稳定性。根据梅奥肘关节功能评分(MEPS),采用这种方法我们获得了良好的结果,并且我们能够减少并发症的发生率。我们的经验还表明,使用平行位置的预轮廓钢板恢复关节面解剖前倾,可以有效地获得肱骨远端骨折的解剖复位。
{"title":"The use of pre-contoured plates in the treatment of C2-C3 fractures of the distal humerus: clinical experience.","authors":"Andrea Celli, Maria Teresa Donini, Claudio Minervini","doi":"10.1007/s12306-007-0022-3","DOIUrl":"10.1007/s12306-007-0022-3","url":null,"abstract":"<p><p>Open reduction and internal fixation is the treatment of choice in distal humerus fractures. The aims of the treatment are to obtain anatomic reconstruction and restoration of the elbow's geometry followed by stable internal fixation that allows immediate rehabilitation. The plates are pre-contoured to fit the natural anatomy of the elbow and in the case of complex fractures they provide a guide for the anatomic restoration of the distal humerus. The two plates are placed parallel with the screws locked together by interdigitation creating a fixed angle in the distal fragments that provides stability to the entire distal humerus. Using this method we obtained good results according to the Mayo Elbow Performance Score (MEPS) and we are able to reduce the incidence of complications. Our experience shows also that it can be useful to obtain an anatomical reduction of distal humeral fractures using the pre-contoured plates in a parallel position recovering the anatomical antiversion of the articular surface.</p>","PeriodicalId":76085,"journal":{"name":"La Chirurgia degli organi di movimento","volume":"91 2","pages":"57-64"},"PeriodicalIF":0.0,"publicationDate":"2008-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27304601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
La Chirurgia degli organi di movimento
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
﹀
mjeditor
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1