Various cellular factors in which their proliferative functions are inter-related (i.e., genes, proteins, miRNA) have been increasingly reported, both in normal cell and cancer. Increase in cellular proliferative rate in cancer is attributed to deregulation of mechanisms related to cell cycle, tumor suppressor and apoptotic control pathways. In this regard, there must be some error occurring within the functional molecules in one or more of these pathways. For instances, gene mutation or amplification, chromosome aberration, epigenetic change, abnormal increase or decrease of some miRNA or derangement of interacting proteins. In breast cancer, alike other cancers, cell cycle driving genes usually express at the level higher than normal and sometimes known as “proliferative or cancer signature” genes. Noteworthy, some cancer-associated genes express at a low level in cancer and are not recognized as the proliferative or cancer signature in spite of their obvious roles on tumorigenesis. The genes include those known to express for cell cycle inhibitors, intercellular adhesive molecules, proteins which function for DNA repairing and genome stability and molecules that contribute in apoptosis. This review gathers and concludes the roles of key molecules believed to be breast cancer associated to date. Cumulative knowledge of molecular cross-talking signals in normal mammary epithelium guides us to understand how deviated molecules and distorted regulations occur in breast cancer. In addition, no single molecule can provide full cellular proliferative function and this is also true in cancer. Hence, targeted therapy for cancer with highly specific inhibitor to such a single molecule expected to be the leading cancer actor is generally not guarantee of the therapeutic successful, and should be performed with careful consideration.
{"title":"Core breast cancer-associated molecules: The Essence","authors":"P. Patmasiriwat","doi":"10.14456/TJG.2013.144","DOIUrl":"https://doi.org/10.14456/TJG.2013.144","url":null,"abstract":"Various cellular factors in which their proliferative functions are inter-related (i.e., genes, proteins, miRNA) have been increasingly reported, both in normal cell and cancer. Increase in cellular proliferative rate in cancer is attributed to deregulation of mechanisms related to cell cycle, tumor suppressor and apoptotic control pathways. In this regard, there must be some error occurring within the functional molecules in one or more of these pathways. For instances, gene mutation or amplification, chromosome aberration, epigenetic change, abnormal increase or decrease of some miRNA or derangement of interacting proteins. In breast cancer, alike other cancers, cell cycle driving genes usually express at the level higher than normal and sometimes known as “proliferative or cancer signature” genes. Noteworthy, some cancer-associated genes express at a low level in cancer and are not recognized as the proliferative or cancer signature in spite of their obvious roles on tumorigenesis. The genes include those known to express for cell cycle inhibitors, intercellular adhesive molecules, proteins which function for DNA repairing and genome stability and molecules that contribute in apoptosis. This review gathers and concludes the roles of key molecules believed to be breast cancer associated to date. Cumulative knowledge of molecular cross-talking signals in normal mammary epithelium guides us to understand how deviated molecules and distorted regulations occur in breast cancer. In addition, no single molecule can provide full cellular proliferative function and this is also true in cancer. Hence, targeted therapy for cancer with highly specific inhibitor to such a single molecule expected to be the leading cancer actor is generally not guarantee of the therapeutic successful, and should be performed with careful consideration.","PeriodicalId":89652,"journal":{"name":"Advances in genomics and genetics","volume":"99 1","pages":"128-140"},"PeriodicalIF":0.0,"publicationDate":"2013-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75531460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Thitiya Poonpet, S. Honsawek, N. Tammachote, S. Kanitnate, R. Tammachote
Osteoarthritis (OA) is the most common chronic joint disease in aging people. It is characterized by the degeneration and loss of the cartilage at the joint surface, accompanied by subchondral bone changes. Since the first characteristic of OA is the loss of collagen network, matrix metalloproteinases (MMPs) with collagenase activity are of interest as the possible cause of OA. The objective of this study was to investigate the association between a deletion SNP in MMP1 promoter (rs1799750; 1G/2G) and knee OA susceptibility in a Thai population. The study subjects consisted of 108 knee OA patients and 119 healthy controls. SNP genotyping was carried out by high resolution melting (HRM) analysis on the CFX96 TM real-time PCR system (Bio-Rad). The melt profiles from all DNA samples were processed by Precision Melt Analysis TM software. The allele frequencies (2G and 1G) and genotype distributions (2G/2G, 2G/1G and 1G/1G) were compared between patients and controls using the StatCalc program. The results showed that all genotypes are normally distributed, and the 2G allele is the major allele in this study population. No statistically significant association was found from all alleles and genotypes tested, both in the whole group and gender stratified groups. In conclusion, a SNP in the promoter of MMP1 was not associated with knee OA susceptibility in a Thai population.
{"title":"Association study of the MMP1 gene polymorphism with knee osteoarthritis in Thais by high resolution melting (HRM) analysis","authors":"Thitiya Poonpet, S. Honsawek, N. Tammachote, S. Kanitnate, R. Tammachote","doi":"10.14456/TJG.2013.115","DOIUrl":"https://doi.org/10.14456/TJG.2013.115","url":null,"abstract":"Osteoarthritis (OA) is the most common chronic joint disease in aging people. It is characterized by the degeneration and loss of the cartilage at the joint surface, accompanied by subchondral bone changes. Since the first characteristic of OA is the loss of collagen network, matrix metalloproteinases (MMPs) with collagenase activity are of interest as the possible cause of OA. The objective of this study was to investigate the association between a deletion SNP in MMP1 promoter (rs1799750; 1G/2G) and knee OA susceptibility in a Thai population. The study subjects consisted of 108 knee OA patients and 119 healthy controls. SNP genotyping was carried out by high resolution melting (HRM) analysis on the CFX96 TM real-time PCR system (Bio-Rad). The melt profiles from all DNA samples were processed by Precision Melt Analysis TM software. The allele frequencies (2G and 1G) and genotype distributions (2G/2G, 2G/1G and 1G/1G) were compared between patients and controls using the StatCalc program. The results showed that all genotypes are normally distributed, and the 2G allele is the major allele in this study population. No statistically significant association was found from all alleles and genotypes tested, both in the whole group and gender stratified groups. In conclusion, a SNP in the promoter of MMP1 was not associated with knee OA susceptibility in a Thai population.","PeriodicalId":89652,"journal":{"name":"Advances in genomics and genetics","volume":"23 1","pages":"181-192"},"PeriodicalIF":0.0,"publicationDate":"2013-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86830598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Gene based SNP markers for oil palm were developed from cloned candidate genes that are involved in fatty acid (FA), triacylglycerol and pyruvate-acetyl CoA synthesis pathways of oil palm. Based on exon/introns splice junctions, a total of 28 specific primer pairs corresponding to 22 gene loci were designed and tested for polymorphism between 23 parents (13 dura and 10 pisifera ) used in an oil palm breeding programme. Single strand conformation polymorphism polyacrylamide gel electrophoresis (SSCP) was used to detect the polymorphism. More than 80% of the gene loci showed clear polymorphic patterns in target parents which progenies of at least 3, up to all 14 crosses would display segregation for the markers. The gene based SNP markers developed in this study do not only provide potential marker tools for further QTL mapping analysis in multiple crosses but can also be used as anchor points for further comparing and integrating oil palm linkage maps developed from individual progenies.
{"title":"Development of gene based markers for mapping analysis in oil palm","authors":"S. Jeennor","doi":"10.14456/TJG.2013.116","DOIUrl":"https://doi.org/10.14456/TJG.2013.116","url":null,"abstract":"Gene based SNP markers for oil palm were developed from cloned candidate genes that are involved in fatty acid (FA), triacylglycerol and pyruvate-acetyl CoA synthesis pathways of oil palm. Based on exon/introns splice junctions, a total of 28 specific primer pairs corresponding to 22 gene loci were designed and tested for polymorphism between 23 parents (13 dura and 10 pisifera ) used in an oil palm breeding programme. Single strand conformation polymorphism polyacrylamide gel electrophoresis (SSCP) was used to detect the polymorphism. More than 80% of the gene loci showed clear polymorphic patterns in target parents which progenies of at least 3, up to all 14 crosses would display segregation for the markers. The gene based SNP markers developed in this study do not only provide potential marker tools for further QTL mapping analysis in multiple crosses but can also be used as anchor points for further comparing and integrating oil palm linkage maps developed from individual progenies.","PeriodicalId":89652,"journal":{"name":"Advances in genomics and genetics","volume":"14 5 1","pages":"168-180"},"PeriodicalIF":0.0,"publicationDate":"2013-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83017897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Charadrius spp. are migratory shorebirds in Thailand during the non-breeding season inwhich adult plumages are not clearly sexually dimorphic. In this work, a molecular approach was used to determine the gender and genetic diversity. Amplification of intron length polymorphisms in chromo-helicase-DNA-binding ( CHD ) genes on both Z and W chromosomes of three Charadrius spp. included 87 lesser sand plover ( Charadrius mongolus ), 27 greater sand plover ( C. leschenaultii ) and 3 kentish plover ( C. alexandrinus ). The resulting PCR products from 2550F/2718R primer showed fragments on a conventional agarose gel electrophoresis with size differences ranging from 200 bp between the two respective ZW allele. Males were identified by the presence of a single band about 650 bp ( CHD-Z ). Heterogametic females were identified by the presence of a second additional fragment length of approximately 450 bp ( CHD-W ). The CHD-Z fragments were purified and analysed by sequencing. The multiple sequence comparison was accomplished using ClustalX and PHYLIP software. These data demonstrate that the CHD-Z sequence related with morphology and can be used for genetic diversity study. The results of molecular sex determination and genetic diversity analyses will also have implications for shorebird conservation.
{"title":"Sexing and genetic diversity in the genus Charadrius based on CHD gene","authors":"S. Poeaim","doi":"10.14456/TJG.2013.114","DOIUrl":"https://doi.org/10.14456/TJG.2013.114","url":null,"abstract":"Charadrius spp. are migratory shorebirds in Thailand during the non-breeding season inwhich adult plumages are not clearly sexually dimorphic. In this work, a molecular approach was used to determine the gender and genetic diversity. Amplification of intron length polymorphisms in chromo-helicase-DNA-binding ( CHD ) genes on both Z and W chromosomes of three Charadrius spp. included 87 lesser sand plover ( Charadrius mongolus ), 27 greater sand plover ( C. leschenaultii ) and 3 kentish plover ( C. alexandrinus ). The resulting PCR products from 2550F/2718R primer showed fragments on a conventional agarose gel electrophoresis with size differences ranging from 200 bp between the two respective ZW allele. Males were identified by the presence of a single band about 650 bp ( CHD-Z ). Heterogametic females were identified by the presence of a second additional fragment length of approximately 450 bp ( CHD-W ). The CHD-Z fragments were purified and analysed by sequencing. The multiple sequence comparison was accomplished using ClustalX and PHYLIP software. These data demonstrate that the CHD-Z sequence related with morphology and can be used for genetic diversity study. The results of molecular sex determination and genetic diversity analyses will also have implications for shorebird conservation.","PeriodicalId":89652,"journal":{"name":"Advances in genomics and genetics","volume":"159 1","pages":"141-149"},"PeriodicalIF":0.0,"publicationDate":"2013-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73277193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Multiplex PCR เหมาะสาหรับการศึกษาระบาดวิทยาของไรฝุ่น","authors":"ณัฐ มาลัยนวล","doi":"10.14456/TJG.2013.16","DOIUrl":"https://doi.org/10.14456/TJG.2013.16","url":null,"abstract":"","PeriodicalId":89652,"journal":{"name":"Advances in genomics and genetics","volume":"11 1","pages":"418"},"PeriodicalIF":0.0,"publicationDate":"2013-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73453893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"การตรวจการกลายของยีน BRAF ในโรคมะเร็งต่อมไทรอยด์","authors":"อัญชลี ไหลพิริยกุล","doi":"10.14456/TJG.2013.82","DOIUrl":"https://doi.org/10.14456/TJG.2013.82","url":null,"abstract":"","PeriodicalId":89652,"journal":{"name":"Advances in genomics and genetics","volume":"134 1","pages":"146-151"},"PeriodicalIF":0.0,"publicationDate":"2013-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88905481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：