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Aspects, Needs and Usefulness of Forests in Human Life from Ancient Time Till Now 古往今来,森林在人类生活中的方面、需要和用途
Pub Date : 2021-01-01 DOI: 10.11648/J.JHER.20210701.15
Gulistan Khairandish
The main objective of writing this paper is to find out the vital important role of forests in humane life and make them aware from such amazing benefits of natural forests. The information all about this topic has been collected from the authentic scientific books and the result show that the vegetation cover, pasture, forests and green space is source of a country. Conservation of water and soil, prevention of soil erosion, increase of ground water resources, providing of the clear air for breathing of organisms, producing of deferent type of fruits, producing of raw materials for woodcraft and environmental protection is the non-forgettable benefits of forests. Malika Hoshmand (2019) says” trees are the lifeblood of humans and animals are in fact the breath of earth, by absorbing toxic gases such as carbon dioxide and producing oxygen, which is one of the most basic needs of humans and animal’s life, and save the natural cycle”. Forests were the home of ancient human and same human today, trees are producing fruits to save humans from hunger, protects soil to keep avoid from huge floods and absorbs dusts to save human heaths.
写这篇论文的主要目的是找出森林在人类生活中的重要作用,并使他们意识到天然森林的惊人好处。有关本课题的所有信息都是从权威的科学书籍中收集的,结果表明植被覆盖、牧场、森林和绿地是一个国家的源泉。保持水土,防止水土流失,增加地下水资源,为生物呼吸提供清新的空气,生产各种水果,生产木材原料,保护环境,是森林不可忘记的效益。Malika Hoshmand(2019)说:“树木是人类和动物的命脉,实际上是地球的呼吸,通过吸收二氧化碳等有毒气体并产生氧气,这是人类和动物生命最基本的需求之一,并拯救了自然循环”。森林是古代人类的家园,也是今天人类的家园,树木结出果实,使人类免于饥饿,保护土壤,防止洪水泛滥,吸收灰尘,拯救人类健康。
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引用次数: 0
An Artificial Intelligence Approach to Modeling in Social Science 社会科学建模的人工智能方法
Pub Date : 2021-01-01 DOI: 10.11648/J.JHER.20210701.20
J. C. Vázquez, J. Castillo, Leticia E. Constable, Marina E. Cardenas, J. C. Vazquez
Computer Science has contributed to social sciences since decades ago: connecting people that build virtual communities where the interactions can be investigated, developing tools for statistically analytics, designing models that allow the analysis and simulation of the most diverse types, among many others. In this article, we describe an artificial neural network to model a theoretical framework for risk, housing, and health problematic, called DRVS (Diagnostic methodology for risk determination of urban housing for health), which uses a holistic approach for community and environmental health. The methodology also exposes digital clinic history for families and communities, developed to support the acquisition of necessary data. This software has advantages for the transference and application of the DRVS in different locations since it constitutes an expert system for the determination of local social indexes and supports the quantitative validation process for the underlying social theory. On the other hand, as many artificial intelligence techniques, it has constraints: unlike explicit logic inferences, artificial neural networks work as «black boxes», not explaining how they got the result; they have a strong dependency of the representativeness of training data and introducing new knowledge that may improve their results and performance is difficult (new data, addition or remotion of determining factors for the underlying social model, weighting factors, etc.). This article also shows some techniques and ideas on how to deal with the identified constraints.
从几十年前起,计算机科学就对社会科学做出了贡献:将人们联系起来,建立虚拟社区,在那里可以调查互动,开发统计分析工具,设计模型,允许对最多样化的类型进行分析和模拟,等等。在本文中,我们描述了一个人工神经网络来模拟风险、住房和健康问题的理论框架,称为DRVS(城市住房健康风险确定诊断方法),它使用了社区和环境健康的整体方法。该方法还暴露了家庭和社区的数字临床病史,为支持必要数据的获取而开发。该软件构成了一个确定当地社会指标的专家系统,为基础社会理论的定量验证过程提供支持,有利于DRVS在不同地区的迁移和应用。另一方面,与许多人工智能技术一样,它也有局限性:与明确的逻辑推理不同,人工神经网络像“黑匣子”一样工作,不解释它们是如何得到结果的;它们对训练数据的代表性有很强的依赖性,引入可能改善其结果和性能的新知识是困难的(新数据,增加或删除底层社会模型的决定因素,加权因素等)。本文还展示了一些关于如何处理已确定的约束的技术和思想。
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引用次数: 0
Hospitals Surfaces and Sites as a Reservoir for Pathogenic Bacteria That Play a Role in Transmission of Infectious Diseases 医院表面和场所作为病原细菌的储存库,在传染病传播中起作用
Pub Date : 2021-01-01 DOI: 10.11648/j.jher.20210703.14
Maina Susan Muthoni
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引用次数: 0
Seasonal Microbial Quality of Drinking Water in Shendi Town, River Nile State, Sudan 苏丹尼罗河州申迪镇饮用水季节性微生物质量研究
Pub Date : 2021-01-01 DOI: 10.11648/j.jher.20210703.17
Abdallah Ahmed Adam Belal, Basheer Mohammed El hassan, Ahmed Mohammed Hussein
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引用次数: 0
Spatial Distribution and Associated Risk Factors of Soil-Transmitted Helminth Infection in a Semi-urban Community in Southwest Nigeria 尼日利亚西南部半城市社区土壤传播蠕虫感染的空间分布及相关危险因素
Pub Date : 2021-01-01 DOI: 10.11648/j.jher.20210704.13
Oluwatoba Olufunke Abiodun, Taiwo Olalekan John, Nwuba Roseangela Ifeyinwa
: Parasite distribution exhibit heterogeneity within households and communities. The aim of this study is to identify the spatial distribution, clustering and associated risk factors of Soil-transmitted Helminths infection in a semi-urban area in Southwest Nigeria. Geographical information System (GIS) was used to map the town into three density areas; low, medium and high density areas. Two hundred and thirty six soil samples were obtained from the surroundings of 137 households and stool samples were collected from the inhabitant of the households. Moran index was used to analyze the spatial distribution of STH within the community. A total of 137 households were sampled for human STH and environmental soil samples from the household were analyzed. The prevalence of human STH infection was 41.7% while that of soil parasite was 27.7% within the selected household. Ascaris lumbricoides was the predominant STH infection in the community sampled with 24.1% prevalence followed by Hookworm. Spatial analysis showed that A. lumbricoides infection was significantly clustered only in the GIS mapped medium density area of Igbo-Ora (z-score 2.302, p=0.021), while hookworm infection was randomly distributed in all the GIS mapped areas of Igbo-Ora. There was a statistically significant association between human STH infection and presence of parasite in the household soil ( x 2 5.74, p-value 0.017). None of the risk factors evaluated was found to have significant association for the clustering pattern of infection found in the medium density area of the community. Meanwhile, factors such as; the physical presence of a popularly patronized local market in the area, lack of any visible public toilet and the use of bushes around the area by the people for defecation contribute to the reasons for the clustering of the Ascaris infection. Also, the ability of Ascaris to withstand environmental conditions than other STH parasite may contribute to the pattern observed. The differences in the pattern of distribution and associated risk factors revealed that environmental conditions and exposure factors are major determinant of STH infection.
寄生虫在家庭和社区中的分布呈现异质性。本研究的目的是确定尼日利亚西南部半城市地区土壤传播蠕虫感染的空间分布、聚集性和相关危险因素。利用地理信息系统(GIS)将城镇划分为三个密度区;低、中、高密度地区。在137户家庭周围采集了236份土壤样本,并采集了住户粪便样本。采用Moran指数分析了STH在社区内的空间分布。共对137个家庭进行了人体STH采样,并对家庭的环境土壤样本进行了分析。家庭内人STH感染率为41.7%,土壤寄生虫感染率为27.7%。以蚓状蛔虫为主,感染率为24.1%,其次为钩虫。空间分析结果显示,蛔虫感染仅在Igbo-Ora的GIS中密度区显著聚集(z-score 2.302, p=0.021),而钩虫感染则随机分布在Igbo-Ora的所有GIS地图区。人类STH感染与家庭土壤中寄生虫存在有统计学意义的相关性(χ 2 5.74, p值0.017)。评估的危险因素均未发现与社区中密度地区感染聚集模式有显著关联。同时,诸如;该地区有一个受欢迎的当地市场,没有任何可见的公共厕所,以及人们在该地区周围的灌木丛中排便,这些都是造成蛔虫感染聚集的原因。此外,蛔虫比其他STH寄生虫更能承受环境条件,这可能是观察到的模式的原因之一。分布模式和相关危险因素的差异表明,环境条件和暴露因素是STH感染的主要决定因素。
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引用次数: 0
Analysis of Pulmonary and Physical Function Three Months After Discharge for Moderate to Severe COVID-19 中重度COVID-19患者出院后3个月肺功能和身体功能分析
Pub Date : 2021-01-01 DOI: 10.11648/j.jher.20210703.15
Bas Floris Maria Van Raaij, Jordy Lauran Stöger, Maarten Sebastiaan Werkman, Michiel Alexander De Graaf, Maria Louisa Antoni, Geert Hendrik Groeneveld, Anna Helena Elvire Roukens, Frederikus Albertus Klok, Soerindra Rajen Soeniel Ramai, Jacomina Jessica Miranda Geelhoed
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引用次数: 0
Effects of School-based Smoking Prevention Education by Physician After Eight Years: A School Randomized Controlled Trial 八年后医师学校预防吸烟教育的效果:一项学校随机对照试验
Pub Date : 2020-12-08 DOI: 10.11648/J.JHER.20200604.13
Endo Masamitsu, Iwaki Norio, Fukuda Tamutsu, Yoshida Kenji, Nomura Hideki, Kido Teruhiko, Takahashi Yuko, Ikeda Michiko, Nakashima Motoko, Morita Masato, Douniwa Kenichi, Kita Toshiyuki
Numerous studies have examined whether school-based smoking prevention education for minors reduces smoking rates, but consensus has not been established. Moreover, there are few reports about non-smoking classes offered by physicians with long-term results. Volunteer doctors have provided tobacco prevention classes in Kanazawa, Japan since 1998. The aim of this article is to assess whether the education by physicians was effective after eight years. A community area having 14 elementary schools was selected for this study. The study groups comprised randomized schools receiving the 45 minutes non-smoking education by physicians for 12-year-old sixth graders once a year and control schools with no intervention during three years from 2007 to 2009. A randomized controlled trial was conducted among 4,014 (1,615 educated and 2,399 control) students. After eight years, a questionnaire survey on the smoking behaviors of 20-year-old young adults within the community was conducted and 1,634 (631 educated and 1,003 control) replies were collected. The smoking rates of the two groups were compared. At the eight-year follow-up, the smoking rates in the educated group significantly decreased compared to those in the control, especially among males. The school-based smoking prevention education by physicians was effective in reducing the smoking rates among 12-year-old students 8 years later.
许多研究调查了以学校为基础的未成年人预防吸烟教育是否能降低吸烟率,但尚未达成共识。此外,很少有关于医生提供的戒烟课程有长期效果的报道。自1998年以来,志愿医生一直在日本金泽提供烟草预防课程。这篇文章的目的是评估医师在8年后的教育是否有效。本研究选取了一个拥有14所小学的社区区域。研究小组包括随机学校,这些学校每年一次接受医生对12岁的六年级学生进行45分钟的禁烟教育,而对照学校在2007年至2009年的三年中没有任何干预。一项随机对照试验在4014名学生中进行(1615名受教育学生和2399名对照组)。8年后,对社区内20岁青年的吸烟行为进行问卷调查,收集回执1634份(受教育者631份,对照组1003份)。比较两组的吸烟率。在8年的随访中,与对照组相比,受教育组的吸烟率显著下降,尤其是男性。以学校为基础的医生预防吸烟教育在8年后有效降低了12岁学生的吸烟率。
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引用次数: 0
The Effects of Socio-cultural Factors on Medical College Students' Self-Rated Health Status and Health-Promoting Lifestyles in Eritrea: A Cross-sectional Study 社会文化因素对厄立特里亚医科大学生自评健康状况和促进健康生活方式的影响:一项横断面研究
Pub Date : 2020-03-02 DOI: 10.21203/rs.3.rs-15692/v1
Isayas Afewerki Abraham, Xu-fu Zhang, Zewdi Amanuel Dagnew, E. Tesfamariam, G. G. Beraki, Oliver Okoth Achila, Ying-chun Dai
Background: Sub-optimal health status (SHS) and unhealthy lifestyle among college students have become a major focus for research on public health worldwide. Hence, this study was designed to assess the effects of socio-cultural factors on medical college students' self-rated health status (SRH) and health-promoting lifestyles (HPL) in Eritrea.Methods: A descriptive cross-sectional study was conducted at Orotta School of Medicine (OSM), using a self-administered questionnaire. Data was collected from all medical students who had fulfilled the inclusion criteria using Sub-Optimal Health Measurement Scale (SHMS V1.0) and Health-Promoting Lifestyle Profile-II (HPLP-II) scale questionnaires. Independent samples t-test, ANOVA, Chi-square test, Pearson’s correlation coefficient and Binary multivariate logistic regression were performed. Data analysis was undertaken using SPSS version 22.Results: The overall SRH was significantly higher among college students with BMI of 25 to 29.9 (M=88.23, SD=6.28) as compared to <18.5 (p=0.027) and 18.5 to 24.9 (p=0.027). Moreover, physiological subscale of SRH was significantly related to gender (M males= 88.96 Vs M females= 85.41, p=0.001) and monthly stipend (M income = 89.52 Vs M no-income = 87.28, p=0.040). On the other hand, physical activity of HPL was found to vary by gender (M males= 2.12 Vs M females=1.87, p=0.005) and alcohol consumption (M drinkers= 2.22 Vs M non-drinkers = 1.99, p=0.016). Nutrition differences of HPL were observed in BMI of 25 to 29.9 (M =2.40, p=0.028), internship students (M=2.15, p=0.027), and monthly income (M=2.11, p=0.029). The overall SRH was significantly correlated with the overall HPL (r=0.493, p<0.001). In-addition, students who had good and excellent HPL had self-rated themselves as almost five times (AOR=4.97, 95% CI: 1.28, 19.32), and were 3.73 times (AOR=3.73, 95% CI: 1.09, 12.80) healthier than those who had poor HPL, respectively.Conclusions: Based on our study, gender, BMI and monthly stipend play a major role in the SRH whereas gender, alcohol consumption, BMI, study year and monthly stipend play a major role in the HPL. Moreover, there was a significant correlation between SRH and HPL in this study. Therefore, our findings provide evidence that support the role of socio-cultural influences’ on SRH and HPL of medical students.
背景:大学生亚理想健康状况和不健康生活方式已成为全球公共卫生研究的热点。因此,本研究旨在评估社会文化因素对厄立特里亚医科大学生自评健康状况(SRH)和促进健康生活方式(HPL)的影响。方法:在Orotta医学院(OSM)进行了一项描述性横断面研究,采用自填问卷。采用次优健康量表(SHMS V1.0)和健康促进生活方式问卷(HPLP-II)对所有符合纳入标准的医学生进行数据收集。进行独立样本t检验、方差分析、卡方检验、Pearson相关系数和二元多元logistic回归。使用SPSS 22进行数据分析。结果:BMI为25 ~ 29.9 (M=88.23, SD=6.28)的大学生总体SRH高于BMI <18.5 (p=0.027)和BMI <18.5 ~ 24.9 (p=0.027)的大学生。此外,SRH生理分量表与性别(男M = 88.96 Vs女M = 85.41, p=0.001)和月收入(男M = 89.52 Vs无收入M = 87.28, p=0.040)显著相关。另一方面,HPL的体力活动因性别(男性M = 2.12 Vs女性M =1.87, p=0.005)和饮酒(饮酒者M = 2.22 Vs非饮酒者M = 1.99, p=0.016)而异。在25 ~ 29.9的BMI (M= 2.40, p=0.028)、实习学生(M=2.15, p=0.027)、月收入(M=2.11, p=0.029)方面观察HPL的营养差异。总SRH与总HPL显著相关(r=0.493, p<0.001)。此外,健康水平良好和优秀的学生比健康水平差的学生分别自我评价健康水平近5倍(AOR=4.97, 95% CI: 1.28, 19.32)和3.73倍(AOR=3.73, 95% CI: 1.09, 12.80)。结论:根据我们的研究,性别、BMI和每月津贴在SRH中起主要作用,而性别、饮酒、BMI、学习年份和每月津贴在HPL中起主要作用。此外,在本研究中,SRH与HPL之间存在显著相关。因此,我们的研究结果提供了支持社会文化影响对医学生的SRH和HPL的作用的证据。
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引用次数: 1
Prevalence and Epidemiological Profile of Accidents with Exposure to Blood Among Health Professionals in Two Hospitals in the North of Togo 多哥北部两家医院医务人员血液接触事故的流行病学概况
Pub Date : 2019-12-05 DOI: 10.11648/J.JHER.20190504.11
Wasungu Bassokla Ditorguena, Djalogue Prisca, Agbobli Yawo Apelete, Dadjo Soukouna Francis, S. A. Dia, M. Fall, E. Didier, Wognin Sangah, N. Mor, Bonny Jean-Sylvain, Sow Mamadou Lamine
Accidents with exposure to blood (AEBs) remain a reality in healthcare settings and are, by their frequency, a major concern for health professionals. This study was conducted to evaluate the prevalence of AEBs history, to identify the types, circumstances and mechanisms of occurrence and to describe the practices of health professionals with respect to AEBs. We conducted a descriptive cross-sectional study over a period of two (02) months (September-October 2018) in two hospitals, the Kara’s teaching hospital and Kara’s regional hospital, both located in the north, 418 kilometers from Lome, economic capital of Togo. The study population was represented by health professionals practicing in the district pediatrics, surgery, gynecology-obstetrics, emergencies and laboratories of the said centers. Were included in the study health professionals presents and available in the above-mentioned services at the time of the survey. Hospital staff not directly involved in patient care (administrative, mortuary staff, vigils, pharmacy salesmen) were excluded from the study. This research was a descriptive-analytical technique using interviews and questionnaires anonymized and adapted in such a way that it meets our objectives. Methods of data analysis were made using the Sphinx V5 software version 5.1.0.2. The Chi-square statistical test was used to compare the proportions with a significance threshold of 5%. The prevalence of AEBs was estimated at 67.6%. The results show that AEBs were frequent among men compared to women (72.7% vs 58.3%), without significant difference. Age, occupational qualification and seniority in the medical profession were significantly associated to AEBs. The most common mechanism of occurrence was the skin break (89.1%). The equipment or sharp objects handled at the time of the accident were a hollow needle (58.8%), and the most incriminated body fluid was blood (71.7%). AEBs are a reality in health care in Togo with a very high prevalence and concern daily all socio-professional categories especially the nurses and the midwives during the care tasks. Exposure is roughly daily, however, the amount of vaccination coverage in these two hospitals is low. In addition, for health care workers to some dangerous actions, such as disposal of used needles, lack of attention to wearing PPE will be accepted in certain circumstances without risk, it is observed.
血液接触事故(aeb)在卫生保健机构中仍然是一个现实,并且由于其频率,是卫生专业人员关注的主要问题。本研究旨在评估AEBs病史的患病率,确定发生的类型、情况和机制,并描述卫生专业人员对AEBs的做法。我们在两家医院(2018年9月至10月)进行了为期两(02)个月的描述性横断面研究,这两家医院分别是卡拉的教学医院和卡拉的地区医院,均位于北部,距离多哥经济首都洛美418公里。研究人群由在上述中心的地区儿科、外科、妇产科、急诊和实验室执业的卫生专业人员代表。被纳入研究的卫生专业人员在调查时出现在上述服务中。不直接参与病人护理的医院工作人员(行政人员、停尸房工作人员、守夜人员、药房销售人员)被排除在研究之外。这项研究是一种描述性分析技术,使用匿名访谈和问卷调查,并以符合我们目标的方式进行调整。采用Sphinx V5软件版本5.1.0.2进行数据分析。采用卡方统计检验,显著性阈值为5%。AEBs的患病率估计为67.6%。结果显示,男性与女性AEBs发生率较高(72.7% vs 58.3%),差异无统计学意义。年龄、职业资格和医学专业年资与AEBs显著相关。最常见的发生机制是皮肤破裂(89.1%)。事故发生时处理的器械或利器以空心针(58.8%)为主,最主要的犯罪体液是血液(71.7%)。在多哥的卫生保健中,急性腹膜炎的发病率非常高,每天都涉及所有社会专业类别,特别是护理任务中的护士和助产士。但是,这两家医院的疫苗接种率很低。此外,据观察,对于卫生保健工作者的一些危险行为,如处置用过的针头,不注意佩戴个人防护装备在某些情况下是可以接受的,没有风险。
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引用次数: 1
Regulation of TGF-β1-Mediated Wnt/β-catenin Pathways Plays an Important Role in EMT-like Transformation Induced by Cadmium TGF-β1介导的Wnt/β-catenin通路调控在镉诱导的emt样转化中起重要作用
Pub Date : 2019-10-11 DOI: 10.11648/J.JHER.20190503.13
Yanlin Zhang, Jiandong Wu, Lulu Ren, Guning Wang, Lihong Yuan, Zhihui Zou
Backgrounds Some studies have reported that different dosage of cadmium (Cd) had a biphasic effect on cells viability, Cd at a dose of more than 20 μM can induce excessive apoptosis which past studies were mainly concentrated on while there has been few reports on a relatively lower dose of Cd causing abnormal proliferation. Kidney is the most sensitive target organ of Cd, however, the mechanism how Cd at a relatively lower dose affects the proliferation of renal tubular epithelial cells (RTEC) is not yet clear at present. Objectives To explore the toxic effect of Cd at a relatively lower dose on RTEC and study the mechanism how the TGF-β1-mediated Wnt/β-catenin pathways involved in EMT-like transformation induced by Cadmium. Methods The RTEC were isolated by mixed collagenase digestion methods and the effect of Cd at different dose on cell viability was detected by MTT assay. The effect of Cd at Hormesis zone dose on the expression of c-myc, cyclinD1, α-SMA, TGF-β1, Wnt and β-catenin were determined by qRT-PCR. Results When exposed for 24h-72h, Cd at a dose of 2.5 μM had maximum proliferation promoting effect. Cd at Hormesis zone dose could up-regulate the expression of cell cycle and proliferation key regulators c-myc and cyclinD1 in an obvious time-dependent manner. Cd at Hormesis zone dose could significantly promote α-SMA, TGF-β1, Wnt and β-catenin mRNA expression. Conclusions Cd at Hormesis zone dose could induce RTEC significant abnormal proliferation and EMT-like transformation. The activation of TGF-β1-mediated Wnt/β-catenin pathways might play a key role in EMT-like transformation induced by Cd at Hormesis zone dose.
已有研究报道不同剂量的镉(Cd)对细胞活力有双相影响,过去的研究主要集中在20 μM以上的镉可诱导细胞过度凋亡,而相对较低剂量的镉引起细胞异常增殖的报道较少。肾脏是Cd最敏感的靶器官,但相对较低剂量Cd影响肾小管上皮细胞(RTEC)增殖的机制目前尚不清楚。目的探讨相对低剂量Cd对RTEC的毒性作用,研究TGF-β1介导的Wnt/β-catenin通路参与镉诱导的emt样转化的机制。方法采用混合胶原酶消化法分离RTEC细胞,采用MTT法检测不同剂量Cd对细胞活力的影响。采用qRT-PCR检测激效区剂量Cd对c-myc、cyclinD1、α-SMA、TGF-β1、Wnt、β-catenin表达的影响。结果暴露24h-72h时,2.5 μM的Cd对细胞增殖的促进作用最大。激效区剂量Cd可上调细胞周期和增殖关键调控因子c-myc和cyclinD1的表达,且呈明显的时间依赖性。激效区剂量Cd可显著促进α-SMA、TGF-β1、Wnt和β-catenin mRNA的表达。结论激效区剂量Cd可诱导RTEC显著异常增殖和emt样转化。TGF-β1介导的Wnt/β-catenin通路的激活可能在激发区剂量Cd诱导的emt样转化中起关键作用。
{"title":"Regulation of TGF-β1-Mediated Wnt/β-catenin Pathways Plays an Important Role in EMT-like Transformation Induced by Cadmium","authors":"Yanlin Zhang, Jiandong Wu, Lulu Ren, Guning Wang, Lihong Yuan, Zhihui Zou","doi":"10.11648/J.JHER.20190503.13","DOIUrl":"https://doi.org/10.11648/J.JHER.20190503.13","url":null,"abstract":"Backgrounds Some studies have reported that different dosage of cadmium (Cd) had a biphasic effect on cells viability, Cd at a dose of more than 20 μM can induce excessive apoptosis which past studies were mainly concentrated on while there has been few reports on a relatively lower dose of Cd causing abnormal proliferation. Kidney is the most sensitive target organ of Cd, however, the mechanism how Cd at a relatively lower dose affects the proliferation of renal tubular epithelial cells (RTEC) is not yet clear at present. Objectives To explore the toxic effect of Cd at a relatively lower dose on RTEC and study the mechanism how the TGF-β1-mediated Wnt/β-catenin pathways involved in EMT-like transformation induced by Cadmium. Methods The RTEC were isolated by mixed collagenase digestion methods and the effect of Cd at different dose on cell viability was detected by MTT assay. The effect of Cd at Hormesis zone dose on the expression of c-myc, cyclinD1, α-SMA, TGF-β1, Wnt and β-catenin were determined by qRT-PCR. Results When exposed for 24h-72h, Cd at a dose of 2.5 μM had maximum proliferation promoting effect. Cd at Hormesis zone dose could up-regulate the expression of cell cycle and proliferation key regulators c-myc and cyclinD1 in an obvious time-dependent manner. Cd at Hormesis zone dose could significantly promote α-SMA, TGF-β1, Wnt and β-catenin mRNA expression. Conclusions Cd at Hormesis zone dose could induce RTEC significant abnormal proliferation and EMT-like transformation. The activation of TGF-β1-mediated Wnt/β-catenin pathways might play a key role in EMT-like transformation induced by Cd at Hormesis zone dose.","PeriodicalId":91558,"journal":{"name":"Journal of safety, health and environmental research","volume":"66 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83626110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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