{"title":"Replication of SARS CoV2 in Pancreatic Cells via ACE2 Cell Surface Receptors","authors":"K. Jamil","doi":"10.18689/mjcrs-1000148","DOIUrl":"https://doi.org/10.18689/mjcrs-1000148","url":null,"abstract":"","PeriodicalId":355232,"journal":{"name":"Madridge Journal of Case Reports and Studies","volume":"63 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116786254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Transgender Healthcare in Medical Curriculum","authors":"D. R","doi":"10.18689/mjcrs-1000147","DOIUrl":"https://doi.org/10.18689/mjcrs-1000147","url":null,"abstract":"","PeriodicalId":355232,"journal":{"name":"Madridge Journal of Case Reports and Studies","volume":"67 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128367767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Telomerase is a cellular reverse transcriptase which stabilizes telomere length by adding hexameric TTAGGG repeats onto the telomeric ends of chromosomes. Expression of telomerase occurs in germ cells and stem cells but not in normal (somatic) cells. However, in many tumors, telomerase is activated, which is one of the immortalization steps. In humans, telomerase expression is regulated by a telomerase subunit called hTERT. The promoter region of the gene that encodes hTERT is located in a CpG island and was shown to be regulated, at least in part, by DNA methylation. In this study, we have analyzed the methylation status of the hTERT gene in primary squamous cell carcinomas, SCC-1 and SCC-2, and HPV+ cervical tissue scrapes, moderate dyskaryosis and mild dyskaryosis. By using the bisulfite genomic sequencing assay, the region between +90 bp and +566 bp within the gene was analyzed. We found that the primary squamous cell carcinoma SCC-1, which consists of 70% carcinoma cells, was more methylated than SCC-2, which consisted of 40% carcinoma cells. However, the number of methylated CpGs in cervical scrapes classified as moderate dyskaryosis and mild dyskaryosis were higher than in SCC-2. In the present study, we demonstrated the importance of DNA methylation profile at hTERT promoter site as a promising biomarker for cervical cancer. Further, it also provides a possible mechanism into the epigenetic regulation of hTERT in various other cancer developments.
端粒酶是一种细胞逆转录酶，它通过在染色体端粒末端添加六聚体TTAGGG重复序列来稳定端粒长度。端粒酶在生殖细胞和干细胞中表达，但在正常(体细胞)细胞中不表达。然而，在许多肿瘤中，端粒酶被激活，这是不朽的步骤之一。在人类中，端粒酶的表达是由端粒酶亚基hTERT调节的。编码hTERT的基因的启动子区域位于CpG岛，并被证明至少部分受到DNA甲基化的调节。在本研究中，我们分析了hTERT基因在原发性鳞状细胞癌、SCC-1和SCC-2、HPV+宫颈组织刮擦、中度核不良和轻度核不良中的甲基化状态。采用亚硫酸氢盐基因组测序法，分析了该基因+90 bp ~ +566 bp之间的区域。我们发现原发性鳞状细胞癌SCC-1的甲基化程度高于SCC-2, SCC-1由70%的癌细胞组成，SCC-1由40%的癌细胞组成。然而，中度核不良和轻度核不良的宫颈刮擦中甲基化CpGs的数量高于SCC-2。在本研究中，我们证明了hTERT启动子位点DNA甲基化谱作为宫颈癌有前景的生物标志物的重要性。此外，它还为hTERT在各种其他癌症发展中的表观遗传调控提供了可能的机制。
{"title":"Studies on the Role of hTERT – a Telomerase Regulator of DNA Methylation in Cervical Cancer","authors":"Kaiser Jamil M Asimuddin, S. Suresh, K. Jamil","doi":"10.18689/MJCRS-1000146","DOIUrl":"https://doi.org/10.18689/MJCRS-1000146","url":null,"abstract":"Telomerase is a cellular reverse transcriptase which stabilizes telomere length by adding hexameric TTAGGG repeats onto the telomeric ends of chromosomes. Expression of telomerase occurs in germ cells and stem cells but not in normal (somatic) cells. However, in many tumors, telomerase is activated, which is one of the immortalization steps. In humans, telomerase expression is regulated by a telomerase subunit called hTERT. The promoter region of the gene that encodes hTERT is located in a CpG island and was shown to be regulated, at least in part, by DNA methylation. In this study, we have analyzed the methylation status of the hTERT gene in primary squamous cell carcinomas, SCC-1 and SCC-2, and HPV+ cervical tissue scrapes, moderate dyskaryosis and mild dyskaryosis. By using the bisulfite genomic sequencing assay, the region between +90 bp and +566 bp within the gene was analyzed. We found that the primary squamous cell carcinoma SCC-1, which consists of 70% carcinoma cells, was more methylated than SCC-2, which consisted of 40% carcinoma cells. However, the number of methylated CpGs in cervical scrapes classified as moderate dyskaryosis and mild dyskaryosis were higher than in SCC-2. In the present study, we demonstrated the importance of DNA methylation profile at hTERT promoter site as a promising biomarker for cervical cancer. Further, it also provides a possible mechanism into the epigenetic regulation of hTERT in various other cancer developments.","PeriodicalId":355232,"journal":{"name":"Madridge Journal of Case Reports and Studies","volume":"28 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126055531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A Novel Technique to Prevent Head and Neck Injuries during Prone Positioning Gyanesh Jain1, Amit Sharma2*, and Lalit Gupta3 1Consultant Anaesthesiologist, Primus Super speciality Hospital, New Delhi, India 2Senior Resident, Department of Anaesthesiology and Intensive Care, Maulana Azad Medical College, New Delhi, India 3Associate Professor, Department of Anaesthesiology and Intensive Care, Maulana Azad Medical College, New Delhi, India
一种防止俯卧位时头颈部损伤的新技术Gyanesh Jain1, Amit Sharma2*， Lalit gupta 1印度新德里Primus超级专科医院麻醉顾问医师2印度新德里毛拉阿扎德医学院麻醉与重症监护部高级住院医师3印度新德里毛拉阿扎德医学院麻醉与重症监护部副教授
{"title":"A Novel Technique to Prevent Head and Neck Injuries during Prone Positioning","authors":"Amit Jain, L. Gupta, A. Sharma","doi":"10.18689/MJCRS-1000145","DOIUrl":"https://doi.org/10.18689/MJCRS-1000145","url":null,"abstract":"A Novel Technique to Prevent Head and Neck Injuries during Prone Positioning Gyanesh Jain1, Amit Sharma2*, and Lalit Gupta3 1Consultant Anaesthesiologist, Primus Super speciality Hospital, New Delhi, India 2Senior Resident, Department of Anaesthesiology and Intensive Care, Maulana Azad Medical College, New Delhi, India 3Associate Professor, Department of Anaesthesiology and Intensive Care, Maulana Azad Medical College, New Delhi, India","PeriodicalId":355232,"journal":{"name":"Madridge Journal of Case Reports and Studies","volume":"20 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127234842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Rajesh R Havaldar Nitin R Ankle, Samanvaya Soni, R. Havaldar
Computed Tomography (CT) is imperative in establishing a diagnosis in sino-nasal disease and in planning surgery. Various CT parameters like the infundibular, osteomeatal, sphenoethmoid and sino-nasal polyposis types were studied in a total of 30 patients i.e. 60 sides by using standard protocol. The study was done over a period of one year in a tertiary care hospital. The infundibular pattern was the commonest with a prevalence of 25%. Various authors in different regions of the world have studied similar patterns and it can be concluded that there is a wide inter and intra regional variation. This also helps us to understand that sino-nasal disease affliction is limited by race and region and hence, a clear cut pattern of prevalence of various parameters is not possible. Hence, this also stresses on the additional use of CT scan in assessing the pattern of pathology in order to achieve better disease clearance thus avoiding morbidity associated with recurrence.
{"title":"Study of various Anatomical and Pathological Parameters using Computed Tomography in Sino-Nasal Disease-a 1-year Hospital-based Observation Study","authors":"Rajesh R Havaldar Nitin R Ankle, Samanvaya Soni, R. Havaldar","doi":"10.18689/MJCRS-1000144","DOIUrl":"https://doi.org/10.18689/MJCRS-1000144","url":null,"abstract":"Computed Tomography (CT) is imperative in establishing a diagnosis in sino-nasal disease and in planning surgery. Various CT parameters like the infundibular, osteomeatal, sphenoethmoid and sino-nasal polyposis types were studied in a total of 30 patients i.e. 60 sides by using standard protocol. The study was done over a period of one year in a tertiary care hospital. The infundibular pattern was the commonest with a prevalence of 25%. Various authors in different regions of the world have studied similar patterns and it can be concluded that there is a wide inter and intra regional variation. This also helps us to understand that sino-nasal disease affliction is limited by race and region and hence, a clear cut pattern of prevalence of various parameters is not possible. Hence, this also stresses on the additional use of CT scan in assessing the pattern of pathology in order to achieve better disease clearance thus avoiding morbidity associated with recurrence.","PeriodicalId":355232,"journal":{"name":"Madridge Journal of Case Reports and Studies","volume":"47 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133453994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nr Ankle Rajesh R Havaldar O Padmavathy PH Patil, Adarsh D Kumar, R. Havaldar
Nasal obstruction is one of the most common rhinological problems. It can be either unilateral or bilateral. Nasal polyposis and inverted papilloma are two differential diagnosis of unilateral nasal obstruction. Both of these are different in terms of clinical features and appearance. A condition of co-existence of both these pathologies in a single nasal cavity is a rare finding. Moreover, radiological assessment often fails to determine the presence of both these pathologies due to the similar appearance on Computed Tomography. Hence a high index of suspicion and complete clearance of the disease with a histopathological confirmation is of paramount importance to avoid disease related morbidity and mortality.
{"title":"Dual Pathology in a Single Nasal Cavity-A Clinical Case Report","authors":"Nr Ankle Rajesh R Havaldar O Padmavathy PH Patil, Adarsh D Kumar, R. Havaldar","doi":"10.18689/MJCRS-1000143","DOIUrl":"https://doi.org/10.18689/MJCRS-1000143","url":null,"abstract":"Nasal obstruction is one of the most common rhinological problems. It can be either unilateral or bilateral. Nasal polyposis and inverted papilloma are two differential diagnosis of unilateral nasal obstruction. Both of these are different in terms of clinical features and appearance. A condition of co-existence of both these pathologies in a single nasal cavity is a rare finding. Moreover, radiological assessment often fails to determine the presence of both these pathologies due to the similar appearance on Computed Tomography. Hence a high index of suspicion and complete clearance of the disease with a histopathological confirmation is of paramount importance to avoid disease related morbidity and mortality.","PeriodicalId":355232,"journal":{"name":"Madridge Journal of Case Reports and Studies","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124033499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Rajesh R Havaldar Nitin R Ankle, Samanvaya Soni, R. Havaldar
Published by Madridge Publishers Abstract The lateral wall of the nose and the paranasal sinuses has intricate relationship with several adjacent structures such that anatomical variations are a very common entity. This urges the operating surgeon to tailor make the proposed endoscopic sinus surgery to every patient, in order to avoid inadvertent injuries and sequelae formation. The key areas being the anterior ethmoid which is the most susceptible for infection of the anterior group of sinuses and the sphenoethmoid recess for the posterior group of sinuses. Identification of these anatomical variations is the key for optimal disease clearance thus preventing morbidity associated with recurrence.
{"title":"Variations in Intranasal Anatomical Parameters and Its Association with Rhinosinusitis – a One Year Hospital based Observational Study","authors":"Rajesh R Havaldar Nitin R Ankle, Samanvaya Soni, R. Havaldar","doi":"10.18689/MJCRS-1000142","DOIUrl":"https://doi.org/10.18689/MJCRS-1000142","url":null,"abstract":"Published by Madridge Publishers Abstract The lateral wall of the nose and the paranasal sinuses has intricate relationship with several adjacent structures such that anatomical variations are a very common entity. This urges the operating surgeon to tailor make the proposed endoscopic sinus surgery to every patient, in order to avoid inadvertent injuries and sequelae formation. The key areas being the anterior ethmoid which is the most susceptible for infection of the anterior group of sinuses and the sphenoethmoid recess for the posterior group of sinuses. Identification of these anatomical variations is the key for optimal disease clearance thus preventing morbidity associated with recurrence.","PeriodicalId":355232,"journal":{"name":"Madridge Journal of Case Reports and Studies","volume":"151 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127285979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Owaisul Haq Zamin Ahmed Kaiser Jamil, S. Joshi, K. Jamil
Published by Madridge Publishers Abstract Background: Diabetic Nephropathy (DN) is one of the complications in patients with prolonged diabetes. Among the genetic risk factors for DN, TNF-α, an anti-inflammatory cytokine is proposed to act in a paracrine/autocrine manner and is hypothesized to be associated with insulin resistance. In the current study, the relationship of the G ↆC variant of the TNF-α gene in patients, associated with other biochemical parameters with DN was investigated.
{"title":"Polymorphism of TNF-α as a Biomarker of Diabetic Nephropathy in patients of Telangana Region","authors":"Owaisul Haq Zamin Ahmed Kaiser Jamil, S. Joshi, K. Jamil","doi":"10.18689/MJCRS-1000141","DOIUrl":"https://doi.org/10.18689/MJCRS-1000141","url":null,"abstract":"Published by Madridge Publishers Abstract Background: Diabetic Nephropathy (DN) is one of the complications in patients with prolonged diabetes. Among the genetic risk factors for DN, TNF-α, an anti-inflammatory cytokine is proposed to act in a paracrine/autocrine manner and is hypothesized to be associated with insulin resistance. In the current study, the relationship of the G ↆC variant of the TNF-α gene in patients, associated with other biochemical parameters with DN was investigated.","PeriodicalId":355232,"journal":{"name":"Madridge Journal of Case Reports and Studies","volume":"113 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"120813030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: Dental implants treatment has been used in the dentistry for the last few years. The present study was conducted to assess effect of osteoporosis on survival rate of dental implants. Materials and methods: The present study was conducted on 54 osteoporosis patients of both genders who underwent dental implants in the last 5 years. Equal number of age and gender matched control was taken. Patients were recalled regularly after 6 months for the evaluation of mobility, pain, infection around the implant, presence of neuropathy and paresthesia. The level of bone loss at both sides of dental implants was measured. Results: 5 patients in group I and 1 in group II had mobility, 4 in group I and 2 in group II had pain, 1 in group I had presence of neuropathy and 2 in group I and 1 in group II had paresthesia. In group I, most common bone type was III seen in 25 patients and in group II was type I seen in 24 patients. The difference was significant (P<0.05). In group I there was 3.4 mm bone loss on mesial side and 3.6 mm on left side. In group II, there was 1.2 mm bone loss on mesial side and 1.4 mm on distal side. The difference was significant (P<0.05). There were 12 dental implants failure in group I and 4 in group II. The difference was significant (P<0.05). Conclusion: Authors suggested that osteoporosis has great impact on survival rate of dental implant. There were more dental implants failures in these patients as compared to controls.
{"title":"To Assess the effect of Osteoporosis on Survival Rate of Dental Implants-A Clinical Study","authors":"Harshad Mahant Manoj Kr Meena Ashish Pandey, Ankit Galav, A. Pandey","doi":"10.18689/MJCRS-1000140","DOIUrl":"https://doi.org/10.18689/MJCRS-1000140","url":null,"abstract":"Background: Dental implants treatment has been used in the dentistry for the last few years. The present study was conducted to assess effect of osteoporosis on survival rate of dental implants. Materials and methods: The present study was conducted on 54 osteoporosis patients of both genders who underwent dental implants in the last 5 years. Equal number of age and gender matched control was taken. Patients were recalled regularly after 6 months for the evaluation of mobility, pain, infection around the implant, presence of neuropathy and paresthesia. The level of bone loss at both sides of dental implants was measured. Results: 5 patients in group I and 1 in group II had mobility, 4 in group I and 2 in group II had pain, 1 in group I had presence of neuropathy and 2 in group I and 1 in group II had paresthesia. In group I, most common bone type was III seen in 25 patients and in group II was type I seen in 24 patients. The difference was significant (P<0.05). In group I there was 3.4 mm bone loss on mesial side and 3.6 mm on left side. In group II, there was 1.2 mm bone loss on mesial side and 1.4 mm on distal side. The difference was significant (P<0.05). There were 12 dental implants failure in group I and 4 in group II. The difference was significant (P<0.05). Conclusion: Authors suggested that osteoporosis has great impact on survival rate of dental implant. There were more dental implants failures in these patients as compared to controls.","PeriodicalId":355232,"journal":{"name":"Madridge Journal of Case Reports and Studies","volume":"25 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125249851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. Thao, Tran Hau Thach Lam, L. H. Doanh, Tran Thi Linh, Trần Hậu Khang
Hematohidrosis is a rare clinical entity of sweating blood. It may occur in an individual who is suffering from extreme levels of stress, strain or any sort of exertion with underlying bleeding disorders. A limited reports of hematohidrosis was published. We describe a case where bloody sweat was discharged from a whole body episodically in a 26-year-old healthy male with no bleeding disorders or any other underlying cause. The patient was reported with no abnormal investigations and has marked improvement in severity with propranolol, diazepam and vitamin C therapy.
{"title":"A Vietnamese Case of Hematohidrosis","authors":"N. Thao, Tran Hau Thach Lam, L. H. Doanh, Tran Thi Linh, Trần Hậu Khang","doi":"10.18689/mjcrs-1000139","DOIUrl":"https://doi.org/10.18689/mjcrs-1000139","url":null,"abstract":"Hematohidrosis is a rare clinical entity of sweating blood. It may occur in an individual who is suffering from extreme levels of stress, strain or any sort of exertion with underlying bleeding disorders. A limited reports of hematohidrosis was published. We describe a case where bloody sweat was discharged from a whole body episodically in a 26-year-old healthy male with no bleeding disorders or any other underlying cause. The patient was reported with no abnormal investigations and has marked improvement in severity with propranolol, diazepam and vitamin C therapy.","PeriodicalId":355232,"journal":{"name":"Madridge Journal of Case Reports and Studies","volume":"38 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125188854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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