{"title":"Understanding the role of GsWRKY transcription factors modulating the biosynthesis of schaftoside in Grona styracifolia","authors":"Fangqin Yu, Zhiqiang Wang, Dingding Shi, Ting Liu, Ying Wang, Ting Peng, S. Zeng","doi":"10.1007/s44281-023-00022-3","DOIUrl":"https://doi.org/10.1007/s44281-023-00022-3","url":null,"abstract":"","PeriodicalId":491988,"journal":{"name":"Horticulture Advances","volume":"139 46","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138953325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract Fruit color influences fruit quality and commodity value. Most longan ( Dimocarpus longan Lour.) varieties have a yellowish-brown or grayish-yellow pericarp, and the discovery of red pericarp (RP) longan expanded the color varieties of longan fruit. Our previous research showed that the red pericarp of RP fruit was mainly caused by anthocyanin accumulation; however, its underlying regulatory mechanism remains unknown. Herein, DlMYB113, an R2R3-MYB transcription factor, was discovered by examining differentially expressed genes in two longan cultivars. Dimocarpus longan MYB (v-myb avian myeloblastosis viral) 113 ( DlMYB113 ) expression was significantly higher in the pericarp and leaves of RP longan than in ‘Shixia’ longan. Sequence alignment analysis revealed two amino acid substitutions in the R3 domain between DlMYB113rp in RP longan and DlMYB113sx in ‘Shixia’ longan. Transient expression of DlMYB113rp significantly increased anthocyanin accumulation in tobacco leaves, whereas DlMYB113sx had negligible effect. Meanwhile, DlMYB113 overexpression promotes anthocyanin accumulation in Arabidopsis and longan calli. Site-directed mutation detection revealed divergence in DlMYB113 function when the R3 repeat 197-position base T was replaced with G, and the 317- and 318-position AT bases were replaced with GA. Our findings indicate that DlMYB113 can regulate anthocyanin production in RP longan, and three mutations in its nucleic acid sequence lead to anthocyanin accumulation, thereby developing molecular markers associated with the anthocyanin accumulation trait in RP longan. This study will facilitate early screening of longan hybrids with desirable fruit color and be significant for breeding new characteristic varieties.
{"title":"DlMYB113 mutation affects anthocyanin accumulation in red pericarp longan (Dimocarpus longan Lour.)","authors":"Ying-Hua Huang, Li-Qin Liu, De-Bao Yi, Yi-Ying Lu, Li-Fei Zhu, Meng-Ya Chen, Yi-Cheng Wang, Jian-Nan Zhou, Xiao-Wen Hu, Yong-Zan Wei, Sheng-You Shi","doi":"10.1007/s44281-023-00014-3","DOIUrl":"https://doi.org/10.1007/s44281-023-00014-3","url":null,"abstract":"Abstract Fruit color influences fruit quality and commodity value. Most longan ( Dimocarpus longan Lour.) varieties have a yellowish-brown or grayish-yellow pericarp, and the discovery of red pericarp (RP) longan expanded the color varieties of longan fruit. Our previous research showed that the red pericarp of RP fruit was mainly caused by anthocyanin accumulation; however, its underlying regulatory mechanism remains unknown. Herein, DlMYB113, an R2R3-MYB transcription factor, was discovered by examining differentially expressed genes in two longan cultivars. Dimocarpus longan MYB (v-myb avian myeloblastosis viral) 113 ( DlMYB113 ) expression was significantly higher in the pericarp and leaves of RP longan than in ‘Shixia’ longan. Sequence alignment analysis revealed two amino acid substitutions in the R3 domain between DlMYB113rp in RP longan and DlMYB113sx in ‘Shixia’ longan. Transient expression of DlMYB113rp significantly increased anthocyanin accumulation in tobacco leaves, whereas DlMYB113sx had negligible effect. Meanwhile, DlMYB113 overexpression promotes anthocyanin accumulation in Arabidopsis and longan calli. Site-directed mutation detection revealed divergence in DlMYB113 function when the R3 repeat 197-position base T was replaced with G, and the 317- and 318-position AT bases were replaced with GA. Our findings indicate that DlMYB113 can regulate anthocyanin production in RP longan, and three mutations in its nucleic acid sequence lead to anthocyanin accumulation, thereby developing molecular markers associated with the anthocyanin accumulation trait in RP longan. This study will facilitate early screening of longan hybrids with desirable fruit color and be significant for breeding new characteristic varieties.","PeriodicalId":491988,"journal":{"name":"Horticulture Advances","volume":" 13","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135192293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract Fruit ripening (FR) is attributed to the selective expression of several genes precisely governed by various specific transcription factors (TFs). The NAC (NAM, ATAF, and CUC) TF, MaNAC029, positively regulated banana ripening by directly inducing ethylene biosynthesis and transcription of fruit quality-related genes. However, its upstream regulatory mechanism still needs to be clarified. Herein, yeast one-hybrid screening revealed that a SQUAMOSA promoter binding protein-like (SPL) TF, MaSPL16, was a potentially upstream regulator of Musa acuminata N A C ( NAM, ATAF, CUC ) 0 2 9 ( MaNAC029 ). Furthermore, gel mobility shift assay revealed that MaSPL16 can directly bound with the “GTAC” element of the MaNAC029 promoter. The gene expression and promoter activity assays demonstrated that Musa acuminata SPL ( SQUAMOSA promoter binding protein-like ) 16 ( MaSPL16 ) expression was inducible by ethylene and ripening. MaSPL16 was localized to the nucleus, displayed a potenial capacity for transcriptional activation of MaNAC029 . More critically, the transient expression of MaSPL16 in bananas accelerated FR via the upregulation of MaNAC029 and its downstream genes. Collectively, the mechanistic basis of a regulatory cascade involving MaSPL16-MaNAC029 that governed ethylene biosynthesis and fruit quality throughout the entire process of banana fruit ripening was unveiled. These outcomes increase the understanding of the gene-transcriptional regulatory mechanisms in FR. They are envisaged to help devise molecular techniques to regulate maturation and improve future fruit quality.
摘要果实成熟是由多种特定转录因子(TFs)精确调控的基因选择性表达的结果。NAC (NAM, ATAF和CUC) TF, MaNAC029通过直接诱导乙烯生物合成和果实品质相关基因转录，正向调控香蕉成熟。但其上游监管机制仍有待厘清。本文通过酵母单杂交筛选发现，SQUAMOSA启动子结合蛋白样(SPL) TF MaSPL16可能是Musa acuminata N a C (NAM, ATAF, CUC) 029 (MaNAC029)的上游调控因子。此外，凝胶迁移率转移实验显示，MaSPL16可以直接结合MaNAC029启动子的“GTAC”元件。基因表达和启动子活性分析表明，乙烯和催熟诱导了Musa acuminata SPL (SQUAMOSA启动子结合蛋白样)16 (MaSPL16)的表达。MaSPL16定位于细胞核，显示出潜在的转录激活MaNAC029的能力。更关键的是，香蕉中MaSPL16的瞬时表达通过上调MaNAC029及其下游基因加速FR。总的来说，涉及MaSPL16-MaNAC029的调控级联的机制基础在香蕉果实成熟的整个过程中控制乙烯生物合成和果实质量。这些结果增加了对FR基因转录调控机制的理解。他们被设想为帮助设计分子技术来调节成熟和提高未来的果实质量。
{"title":"MaSPL16 positively regulates fruit ripening in bananas via the direct transcriptional induction of MaNAC029","authors":"Wei Wei, Ying-ying Yang, Chao-jie Wu, Jian-fei Kuang, Jian-ye Chen, Wei Shan","doi":"10.1007/s44281-023-00013-4","DOIUrl":"https://doi.org/10.1007/s44281-023-00013-4","url":null,"abstract":"Abstract Fruit ripening (FR) is attributed to the selective expression of several genes precisely governed by various specific transcription factors (TFs). The NAC (NAM, ATAF, and CUC) TF, MaNAC029, positively regulated banana ripening by directly inducing ethylene biosynthesis and transcription of fruit quality-related genes. However, its upstream regulatory mechanism still needs to be clarified. Herein, yeast one-hybrid screening revealed that a SQUAMOSA promoter binding protein-like (SPL) TF, MaSPL16, was a potentially upstream regulator of Musa acuminata N A C ( NAM, ATAF, CUC ) 0 2 9 ( MaNAC029 ). Furthermore, gel mobility shift assay revealed that MaSPL16 can directly bound with the “GTAC” element of the MaNAC029 promoter. The gene expression and promoter activity assays demonstrated that Musa acuminata SPL ( SQUAMOSA promoter binding protein-like ) 16 ( MaSPL16 ) expression was inducible by ethylene and ripening. MaSPL16 was localized to the nucleus, displayed a potenial capacity for transcriptional activation of MaNAC029 . More critically, the transient expression of MaSPL16 in bananas accelerated FR via the upregulation of MaNAC029 and its downstream genes. Collectively, the mechanistic basis of a regulatory cascade involving MaSPL16-MaNAC029 that governed ethylene biosynthesis and fruit quality throughout the entire process of banana fruit ripening was unveiled. These outcomes increase the understanding of the gene-transcriptional regulatory mechanisms in FR. They are envisaged to help devise molecular techniques to regulate maturation and improve future fruit quality.","PeriodicalId":491988,"journal":{"name":"Horticulture Advances","volume":"101 7","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135390060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract Grape production in China is significantly impacted by white rot disease, which is caused by Coniella diplodiella (Speg.) Sacc . This study analyzes the differences in leaf transcriptomes and phenotypes of two grape species, ‘Manicure Finger ( Vitis vinifera L.)’ and ‘0940 ( Vitis davidii Foex)’, following inoculation with C. diplodiella . Leaf anatomy and H 2 O 2 content confirm the greater resistance of '0940' to C. diplodiella compared to 'Manicure Finger.' Comparative transcriptome analysis reveals that the defense mechanism of '0940' against C. diplodiella involves sesquiterpenoid and triterpenoid biosynthesis, plant-pathogen interactions, sulfur relay systems, suberin and wax biosynthesis, monoterpenoid biosynthesis, as well as flavonoid and flavonol biosynthesis pathways. Using Weighted Gene Co-expression Network Analysis (WGCNA), we identified three modules highly correlated with C. diplodiella resistance and 125 candidate genes, including resistant genes ( R genes), pattern-recognition receptors ( PRR s), and pathogenesis-related proteins genes ( PR genes), which may play important roles in grape resistance to this disease.
{"title":"Transcriptome analysis of resistant and susceptible grapes reveals molecular mechanisms underlying resistance of white rot disease","authors":"Peng Li, Xibei Tan, None Wanghao, Lei Sun, Jianfu Jiang, Xiucai Fan, Chonghuai Liu, Ying Zhang","doi":"10.1007/s44281-023-00011-6","DOIUrl":"https://doi.org/10.1007/s44281-023-00011-6","url":null,"abstract":"Abstract Grape production in China is significantly impacted by white rot disease, which is caused by Coniella diplodiella (Speg.) Sacc . This study analyzes the differences in leaf transcriptomes and phenotypes of two grape species, ‘Manicure Finger ( Vitis vinifera L.)’ and ‘0940 ( Vitis davidii Foex)’, following inoculation with C. diplodiella . Leaf anatomy and H 2 O 2 content confirm the greater resistance of '0940' to C. diplodiella compared to 'Manicure Finger.' Comparative transcriptome analysis reveals that the defense mechanism of '0940' against C. diplodiella involves sesquiterpenoid and triterpenoid biosynthesis, plant-pathogen interactions, sulfur relay systems, suberin and wax biosynthesis, monoterpenoid biosynthesis, as well as flavonoid and flavonol biosynthesis pathways. Using Weighted Gene Co-expression Network Analysis (WGCNA), we identified three modules highly correlated with C. diplodiella resistance and 125 candidate genes, including resistant genes ( R genes), pattern-recognition receptors ( PRR s), and pathogenesis-related proteins genes ( PR genes), which may play important roles in grape resistance to this disease.","PeriodicalId":491988,"journal":{"name":"Horticulture Advances","volume":"3 7","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135869259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-30DOI: 10.1007/s44281-023-00012-5
Junren Meng, Shihang Sun, Ang Li, Lei Pan, Wenyi Duan, Guochao Cui, Juan Xu, Liang Niu, Zhiqiang Wang, Wenfang Zeng
Abstract Red leaves in autumn are characteristic of a very early-maturing ripening variety of peach ( Prunus persica ). Analysis of the genetic factors and molecular mechanisms associated with the red-leaf phenotype can help breed peach very early maturing peach varieties. This study investigated the mechanisms underlying the red- and green-leaf phenotypes in autumn. Red compounds accumulated in the older but not younger leaves in the extremely early-maturing peach variety, ‘99-30-33’, and the leaves of the medium-maturing variety, ‘Zhongtao5 (CP5)’. Metabolic analysis showed that cyanidin-3- O -glucoside was the most abundant anthocyanin in the red leaves. The segregation of the progenies obtained from crossing revealed that the red leaves are a unique hereditary phenomenon not in line with Mendel’s law. BSA-seq and RNA-seq analyses suggest that PpNAC1 was essential for enhancing anthocyanin biosynthesis and was highly upregulated in red than in green leaves. Similar to PpNAC1 , the anthocyanin activator, PpMYB10.1 , was the only gene highly expressed in red leaves. Moreover, the functional genes involved in anthocyanin biosynthesis, such as Prunus persica Flavonoid 3'-hydroxylase ( PpF3'H ), Prunus persicaDihydroflavonol reductase ( PpDFR ), Prunus persica Leucoantho-cyanidin dioxygenase ( Pp LDOX ), Prunus persica Glutathione S-transferase ( PpGST ), and Prunus persica UDP-glucose , flavonoid-3-O-glucosyltransferase ( PpUFGT ), were upregulated in the older red leaves of 99-30-33 but downregulated in the younger 99-30-33 and green CP5 leaves. Yeast one-hybrid and dual-luciferase assays further confirmed that PpNAC1 , which refers to ' Prunus persica NAC ( NAM、A TAF1/2、CUC1/2 )' bound to the promoter of PpMYB10.1 , PpMYB10.1 stands for ' Prunus persica MYB ( v-myb avian myeloblastosis viral ) 10.1 ' and activated its expression along with those of PpGST and PpUFGT. These results provide insights into the mechanisms responsible for the development of red color in peach leaves in autumn.
秋天的红叶是桃(Prunus persica)早熟成熟品种的特征。分析与红叶表型相关的遗传因素和分子机制，有助于桃极早熟品种的选育。本研究探讨了秋季红叶和绿叶表型的机制。极早熟品种‘99-30-33’和中熟品种‘中桃5号(CP5)’的叶片中红色化合物在老叶中积累，而在幼叶中不积累。代谢分析表明，花青素-3- O -葡萄糖苷是红叶中含量最多的花青素。杂交获得的后代分离表明，红叶是一种独特的遗传现象，不符合孟德尔定律。BSA-seq和RNA-seq分析表明，PpNAC1在促进花青素生物合成中是必需的，并且在红色叶片中比在绿色叶片中表达水平更高。与PpNAC1相似，花青素激活因子PpMYB10.1是红叶中唯一高表达的基因。此外，参与花青素生物合成的功能基因，如桃李类黄酮3′-羟化酶(ppf3′h)、桃李二氢黄酮醇还原酶(PpDFR)、桃李亮色-花青素双加氧酶(Pp LDOX)、桃李谷胱甘肽s -转移酶(PpGST)和桃李udp -葡萄糖类黄酮-3- o-葡萄糖基转移酶(ppfgt)，在桃李99-30-33老红叶中表达上调，而在桃李99-30-33嫩叶和绿CP5叶中表达下调。酵母单杂交和双荧光素酶实验进一步证实，PpNAC1 (Prunus persica NAC (NAM, A TAF1/2, CUC1/2))与PpMYB10.1的启动子结合，PpMYB10.1代表‘Prunus persica MYB (v-myb禽成髓细胞病病毒)10.1’，并激活了PpGST和pppufgt的表达。这些结果为了解秋季桃叶红色形成的机制提供了新的思路。
{"title":"A NAC transcription factor, PpNAC1, regulates the expression of PpMYB10.1 to promote anthocyanin biosynthesis in the leaves of peach trees in autumn","authors":"Junren Meng, Shihang Sun, Ang Li, Lei Pan, Wenyi Duan, Guochao Cui, Juan Xu, Liang Niu, Zhiqiang Wang, Wenfang Zeng","doi":"10.1007/s44281-023-00012-5","DOIUrl":"https://doi.org/10.1007/s44281-023-00012-5","url":null,"abstract":"Abstract Red leaves in autumn are characteristic of a very early-maturing ripening variety of peach ( Prunus persica ). Analysis of the genetic factors and molecular mechanisms associated with the red-leaf phenotype can help breed peach very early maturing peach varieties. This study investigated the mechanisms underlying the red- and green-leaf phenotypes in autumn. Red compounds accumulated in the older but not younger leaves in the extremely early-maturing peach variety, ‘99-30-33’, and the leaves of the medium-maturing variety, ‘Zhongtao5 (CP5)’. Metabolic analysis showed that cyanidin-3- O -glucoside was the most abundant anthocyanin in the red leaves. The segregation of the progenies obtained from crossing revealed that the red leaves are a unique hereditary phenomenon not in line with Mendel’s law. BSA-seq and RNA-seq analyses suggest that PpNAC1 was essential for enhancing anthocyanin biosynthesis and was highly upregulated in red than in green leaves. Similar to PpNAC1 , the anthocyanin activator, PpMYB10.1 , was the only gene highly expressed in red leaves. Moreover, the functional genes involved in anthocyanin biosynthesis, such as Prunus persica Flavonoid 3'-hydroxylase ( PpF3'H ), Prunus persicaDihydroflavonol reductase ( PpDFR ), Prunus persica Leucoantho-cyanidin dioxygenase ( Pp LDOX ), Prunus persica Glutathione S-transferase ( PpGST ), and Prunus persica UDP-glucose , flavonoid-3-O-glucosyltransferase ( PpUFGT ), were upregulated in the older red leaves of 99-30-33 but downregulated in the younger 99-30-33 and green CP5 leaves. Yeast one-hybrid and dual-luciferase assays further confirmed that PpNAC1 , which refers to ' Prunus persica NAC ( NAM、A TAF1/2、CUC1/2 )' bound to the promoter of PpMYB10.1 , PpMYB10.1 stands for ' Prunus persica MYB ( v-myb avian myeloblastosis viral ) 10.1 ' and activated its expression along with those of PpGST and PpUFGT. These results provide insights into the mechanisms responsible for the development of red color in peach leaves in autumn.","PeriodicalId":491988,"journal":{"name":"Horticulture Advances","volume":"44 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136280346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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