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Effect of Herbal Additives on the Microbiological Profile of Dairy Yoghurts 草药添加剂对乳酪微生物概况的影响
Pub Date : 2024-02-19 DOI: 10.21603/1019-8946-2024-1-2
Igor Bakin, Anna Korchuganova, Dmitry Bychkov, Anna Mustafina
Increasing the survival time and count of viable lactic acid bacteria during storage is an important factor in the probiotic industry. Vegetable powders combined with Lactobacillus bulgaricus and Streptococcus thermophilus can inhibit the growth and reproduction of harmful bacteria in yogurt. This research featured the survival rate of lactic acid bacteria and the microbiological safety of dairy yoghurts fortified with vegetable powders. The research objective was to establish the effect of the additive on the total lactic acid bacterial count in yogurts in order to compare the microbiological indicators with the standards for fortified yogurts. The yoghurts were made by tank method at the municipal dairy plant in Yurga, Kemerovo region. A thermophilic YoFlex culture with Lactobacillus bulgaricus and Streptococcus thermophilus entered the product at the fermentation stage together with dry tomato, fenugreek, and basil powders. The quality tests were conducted at the production laboratory in line with GOST State Standard 31981-2013. All the measurements were done in triplicates, with a significance level of 95 % (p < 0.05). The combined effect of the lactic acid bacteria and the vegetable powders inhibited the growth of E. coli bacteria. The biggest count of viable lactic acid bacteria belonged to the samples fortified with tomato and basil powder. On storage day 1, this sample showed a 62 % higher quantity of mesophilic aerobic and facultative anaerobic microorganisms. Fenugreek and basil leaf powders also increased the viability of yogurt starter cultures compared to the control sample. The number of viable probiotic cells decreased on storage day 8 at 4 ± 2 °C, the recommended shelf life for the new fortified yogurt being 5 days.
在益生菌行业中,提高乳酸菌在储存过程中的存活时间和数量是一个重要因素。添加了保加利亚乳杆菌和嗜热链球菌的蔬菜粉可以抑制酸奶中有害细菌的生长和繁殖。这项研究的特点是乳酸菌的存活率和添加了蔬菜粉的乳制品酸奶的微生物安全性。研究目的是确定添加剂对酸奶中乳酸菌总数的影响,以便将微生物指标与强化酸奶的标准进行比较。酸奶是在克麦罗沃州尤尔加的市乳品厂用罐装法制作的。在发酵阶段,含有保加利亚乳杆菌和嗜热链球菌的嗜热 YoFlex 培养物与番茄干粉、葫芦巴粉和罗勒粉一起进入产品中。质量检测在生产实验室进行,符合 GOST 31981-2013 国家标准。所有测量均采用三重法,显著性水平为 95 %(p < 0.05)。乳酸菌和蔬菜粉的共同作用抑制了大肠杆菌的生长。添加了番茄和罗勒粉的样品中乳酸菌的数量最多。在贮藏第 1 天,该样品中的嗜中性需氧和兼性厌氧微生物数量增加了 62%。与对照样品相比,葫芦巴粉和罗勒叶粉还能提高酸奶启动培养物的活力。在 4±2 °C的条件下储存到第 8 天时,益生菌细胞的存活数量有所减少,而新强化酸奶的建议保质期为 5 天。
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引用次数: 0
Freeze-Dried Mare’s Colostrum 冻干母马牛初乳
Pub Date : 2024-02-19 DOI: 10.21603/1019-8946-2024-1-5
Roza T. Timakova, Yu.V. Iliukhina, V. Startsev
People do not consume raw mare’s colostrum. However, colostrum powder can be used as a biologically active additive to fortify functional foods. Mare’s colostrum is a unique natural multicomponent and multifunctional product with valuable immunomodulatory and regenerating properties that can be used therapeutically. This research featured optimal technological parameters of freeze-drying in the production of mare’s colostrum powder at a field-to-spoon full-cycle farm that breeds Vyatka horses. Low-pressure freeze-drying affects the quality of colostrum powder and its nutritional profile. No State Standard has been developed for mare’s colostrums so far. In fact, the only currently standardized colostrum in Russia is that of cows. In the absence of normative documents, the research had to rely on Technical Regulations of Customs Union TR CU 033/2013, State Standard GOST R 52975-2008, and Technical Specifications TS 10.51.56-001-0133214532-2021. Domestic sublimators with automatic control proved quite efficient in sublimation and drying of mare’s colostrum. The new technology requires no additional purification stage because mare’s colostrum possesses antimicrobial properties. The resulting colostrum powder appeared to have a remarkably long shelf-life. A further in-depth study is needed to reveal the nutrient composition of mare’s colostrum powder in connection with its immunomodulatory protein complex.
人们不会食用未加工的母马初乳。但是,牛初乳粉可以作为一种生物活性添加剂,用于强化功能性食品。母马初乳是一种独特的天然多成分多功能产品,具有宝贵的免疫调节和再生特性,可用于治疗。这项研究的特点是在一个饲养维亚特卡马的从田间到勺子全周期农场生产母马初乳粉时采用最佳的冷冻干燥技术参数。低压冷冻干燥会影响牛初乳粉的质量及其营养成分。迄今为止,尚未制定母马初乳的国家标准。事实上,俄罗斯目前唯一标准化的牛初乳是奶牛的牛初乳。在没有规范性文件的情况下,研究只能依靠《关税同盟技术条例》(TR CU 033/2013)、国家标准《GOST R 52975-2008》和《技术规范》(TS 10.51.56-001-0133214532-2021)。事实证明,具有自动控制功能的国产升华机在升华和干燥母马初乳方面非常有效。由于母马初乳具有抗菌特性,因此新技术不需要额外的净化阶段。制成的牛初乳粉末似乎具有很长的保质期。要揭示母马初乳粉的营养成分及其免疫调节蛋白复合物,还需要进一步深入研究。
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引用次数: 0
Domestic Self-Sufficiency in Milk-Converting Enzymes 国内牛奶转化酶的自给自足
Pub Date : 2024-02-19 DOI: 10.21603/1019-8946-2024-1-10
Olga Musina, Anastasiya Grishkova, A. Prosekov
Food security is part of national security, self-sufficiency in basic foodstuffs being its important indicator. The situation with cheese production raw materials in the Russian Federation is gradually improving. According to the Ministry of Agriculture, the production of raw milk is growing, which means that the level of self-sufficiency is gradually increasing. However, milk-converting enzymes can create a bottleneck situation since the entire rennet cheese production depends on them. The share of imported milk-coagulating enzyme preparations on the Russian market is 84.3 %. Recombinant milk-converting enzymes dominate both domestic and global cheese-making. However, Russia has neither microbial recombinant chymosins nor coagulants of its own. The domestic commercial production of milk-converting enzymes, especially of modern recombinant preparations, is one of the most relevant issues the domestic food industry will have to tackle in the nearest future.
粮食安全是国家安全的一部分,基本食品的自给自足是其重要指标。俄罗斯联邦奶酪生产原料的情况正在逐步改善。据农业部称,原料奶的产量正在增长,这意味着自给自足的水平正在逐步提高。然而,牛奶转化酶会造成瓶颈状况,因为整个凝乳酶奶酪生产都依赖于它们。进口牛奶凝固酶制剂在俄罗斯市场上的份额为 84.3%。重组牛奶转化酶在国内和全球奶酪生产中都占主导地位。然而,俄罗斯既没有微生物重组糜蛋白酶,也没有自己的凝固剂。国内商业化生产牛奶转化酶,特别是现代重组制剂,是国内食品工业在不久的将来必须解决的最相关问题之一。
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引用次数: 0
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