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Silicoflagellates and other siliceous micro- and nannofossils from Rupelian diatomites, southeastern Poland 波兰东南部鲁佩尔硅藻土中的硅鞭毛虫及其他硅质微化石和化石
Q2 Agricultural and Biological Sciences Pub Date : 2024-07-01 DOI: 10.35535/pfsyst-2024-0009
I. Kaczmarska, J. Ehrman
The Eocene-Oligocene Transition is one of the major stages of the Cenozoic reshaping of the world oceans with associated changes in climate, of ocean coastlines and circulation patterns. This altogether created challenging environments for fossilization and preservation of native biota. Consequently, well preserved Oligocene fossils are infrequent worldwide and just as rare in the Central Paratethys. The well-preserved Rupelian (Early Oligocene) diatomites from the Skole Nappe, the external unit of the Outer Carpathians in southeastern Poland were deposited in the Central Paratethys. In some samples, in addition to diatoms, sediments also contain a great diversity of other silicified micro- and nannofossils. Here we report our findings of silicoflagellates (fifteen taxa from five genera), two rotosphaeridians, and several morphotypes of fossil scales, some of uncertain taxonomic affinity. Among silicoflagellates, the most common are species from genera Corbisema (C. triacantha, C. hastata, and C. apiculata) and Dictyocha (D. clinata and D. fibula). We also recovered scales of rotosphaeridians (Pinaciophora and possibly Rabdiophrys) and remains of organisms of uncertain affinity (Clathropyxidella and Macrora) often reported together with silicoflagellates. All of these taxa are the first reports for the Central Paratethys. Silicoflagellate species composition is consistent with the datums derived from other fossils. Silicoflagellates, marine diatoms and archaeomonads suggest a neritic depositional environment.
始新世-渐新世过渡是新生代重塑世界海洋的主要阶段之一,伴随着气候、海洋海岸线和环流模式的变化。这为本地生物群的化石化和保存创造了极具挑战性的环境。因此,保存完好的渐新世化石在全球范围内并不多见,在中帕拉特提斯地区也同样罕见。波兰东南部外喀尔巴阡山脉外部单元 Skole Nappe 中保存完好的 Rupelian(始新世早期)硅藻土沉积于中帕拉泰西。在一些样本中,除了硅藻之外,沉积物还含有种类繁多的其他硅化微小化石和化石。在此,我们报告了硅鞭毛虫(5 个属 15 个类群)、2 个轮虫和几种形态的鳞片化石,其中一些化石的分类亲缘关系尚不确定。在硅鞭毛虫中,最常见的是 Corbisema 属(C. triacantha、C. hastata 和 C. apiculata)和 Dictyocha 属(D. clinata 和 D. fibula)的物种。我们还发现了轮虫(Pinaciophora,可能还有 Rabdiophrys)的鳞片,以及通常与硅鞭毛虫一起报道的亲缘关系不明的生物(Clathropyxidella 和 Macrora)的残骸。所有这些类群都是中帕拉特提斯地区的首次报道。硅鞭毛虫的物种组成与其他化石的数据一致。硅鞭毛虫、海洋硅藻和古单胞菌表明这里是一个海相沉积环境。
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引用次数: 0
Colour range of euglenoid (Euglenophyceae) blooms 类八仙花(八仙花科)花朵的颜色范围
Q2 Agricultural and Biological Sciences Pub Date : 2024-07-01 DOI: 10.35535/pfsyst-2024-0010
Konrad Wołowski, Kritsana Duangjan, Thomas Dempster, P. Tsarenko, M. Poniewozik, J. Koreivienė
The presence of autotrophs, heterotrophs and mixotrophs among euglenoid taxa, multiple means of nutrition, and high tolerance to a wide range of thermal living conditions contribute to their colonization of various types of water bodies in different climatic zones. The formation of water “blooms” by euglenoids occurs during accelerated development and intensive cell division of these organisms. Euglenoids outcompete other microalgae and produce significant biomass under favorable conditions. The ability to produce temporal, reproductive and protective cysts also contributes to rapid bloom formations. Our observations suggest that their features probably make them model organisms for observing environmental changes in small water bodies. So far, little attention has been given to the species-dependent color variation of euglenoid blooms. Long-time research on euglenoid taxonomy, biogeography and habitat observations allow observers to recognize different blooms depending on the species causing the phenomenon: Euglena sanguinea, E. mutabilis, E. clara, E. hemichromata, E. geniculata, E. agilis, Euglenaria anabaena, Euglenaformis chlorophoenica, Monomorphina pyrum, Phacus pleuronectes, Ph. orbicularis, Lepocinclis ovum, Trachelomonas volvocina, T. volvocinopsis and T. caudata. The color range of euglenoid blooms are presented here for the first time.
藻类类群中存在自养型、异养型和混养型生物,它们有多种营养方式,对各种热生活条件有很强的耐受性,这有助于它们在不同气候带的各类水体中定居。藻类在加速发育和密集的细胞分裂过程中形成水体 "藻华"。在有利的条件下,藻类会超越其他微藻,产生大量生物量。产生时间性、生殖性和保护性包囊的能力也有助于快速形成藻华。我们的观察结果表明,它们的特征很可能使其成为观察小水体环境变化的模式生物。迄今为止,人们很少关注藻华的颜色变化与物种有关。通过长期对褐优藻类分类学、生物地理学和栖息地观察的研究,观察者可以根据引起藻华现象的物种识别不同的藻华:Euglena sanguinea、E. mutabilis、E. clara、E. hemichromata、E. geniculata、E. agilis、Euglenaria anabaena、Euglenaformis chlorophoenica、Monomorphina pyrum、Phacus pleuronectes、Ph. orbicularis、Lepocinclis ovum、Trachelomonas volvocina、T. volvocinopsis 和 T. caudata。这里首次介绍了鳗鲡花的颜色范围。
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引用次数: 0
The lichen genera Gondwania and Transdrakea gen. nov. (Teloschistaceae) - speciation in three southern continents 地衣属 Gondwania 和 Transdrakea gen.
Q2 Agricultural and Biological Sciences Pub Date : 2023-12-29 DOI: 10.35535/pfsyst-2023-0015
Ulrik Søchting, L. G. Sancho, U. Arup
Within the lichen family Teloschistaceae, a high number of genera are restricted to the extreme southern part of the Southern Hemisphere. The taxonomy and phylogeny of the southern genus Gondwania have been analyzed based upon fieldwork in Antarctica, Patagonia, New Zealand and Tasmania and subsequent studies including molecular methods. Five species are accepted in the genus Gondwanea including G. inclinans and G. joannae that are combined into the genus. Two species are included in the neighboring new genus Transdrakea, T. alacalufes, a saxicolous species from Patagonia, which is described as new to science and T. schofieldii. Based on molecular data, Austroplaca imperialis is described as a new species to accommodate Patagonian specimens previously named G. regalis. Even though morphologically very similar, the two species are distinct based on molecular and chemical characters and distribution. Gondwania and Transdrakea are chemically homogenous with parietin as the very dominant compound; Transdrakea has a very reduced thallus, whereas Gondwania includes species with crustose thalli, as well as species with fruticulose growth habits. G. inclinans and G. cribrosa are only known from Australia/New Zealand. G. regalis, G. joannae and T. schofieldii are only known from Antarctica. G. sublobulata is confirmed from Patagonia and the Falkland Islands, but Antarctic specimens previously named G. sublobulata belong to G. joannae. G. sejongensis is shown to be a synonym to G. joannae. A key to Gondwania and Transdrakea species is presented.
在地衣科(Teloschistaceae)地衣属中,有很多属只分布在南半球的最南端。根据在南极洲、巴塔哥尼亚、新西兰和塔斯马尼亚进行的实地考察以及随后进行的包括分子方法在内的研究,我们分析了南部冈瓦纳属(Gondwania)的分类和系统发育。Gondwanea 属中有五个物种被接受,其中包括 G. inclinans 和 G. joannae,它们被合并到该属中。两个物种被归入邻近的新属 Transdrakea,即 T. alacalufes(一种产自巴塔哥尼亚的穴居物种)和 T. schofieldii。根据分子数据,Austroplaca imperialis 被描述为一个新物种,以适应以前被命名为 G. regalis 的巴塔哥尼亚标本。尽管在形态上非常相似,但根据分子和化学特征以及分布,这两个物种是不同的。Gondwania 和 Transdrakea 的化学成分相同,叶绿素是最主要的化合物;Transdrakea 的菌柄非常细小,而 Gondwania 包括具有壳质菌柄的物种,以及具有纤毛生长习性的物种。G. inclinans 和 G. cribrosa 只产于澳大利亚/新西兰。G. regalis、G. joannae 和 T. schofieldii 只产于南极洲。sublobulata 证实产自巴塔哥尼亚和福克兰群岛,但之前命名为 G. sublobulata 的南极标本属于 G. joannae。joannae 的异名。Gondwania 和 Transdrakea 物种的检索表。
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引用次数: 0
The lichenized and lichenicolous fungi of Afghanistan 阿富汗的地衣和地衣真菌
Q2 Agricultural and Biological Sciences Pub Date : 2023-12-29 DOI: 10.35535/pfsyst-2023-0026
Helmut Mayrhofer, Karin Plattner, O. Breuss, Kerry Knudsen, Mohammad Sohrabi, M. D. Rafiqpoor, S. Breckle
The study is based on a comprehensive evaluation of 87 publications concerning reports from Afghanistan and a critical revision of herbarium specimens. Two hundred and thirty eight taxa (230 species with 8 varieties) of lichenized fungi, and 16 species of lichenicolous fungi are listed with their citations or localities in the case of new records for the country or for provinces. New for Afghanistan are Acarospora bicolor, A. interrupta, Caloplaca monacensis, C. stillicidiorum var. muscorum, Collema furfuraceum, Endocarpon pusillum, Gloeoheppia turgida, Gyalolechia bracteata, Heteroplacidium fusculum, Lecanora horiza, Lecidea laboriosa, Melanohalea elegantula, Myriolecis semipallida, Peltula bolanderi, P. obscurans var. obscurans, P. obscurans var. deserticola, P. polyspora, P. radicata, Physconia americana, P. perisidiosa, Phaeophyscia hirsuta, Placidium pilosellum, P. rufescens, Sarcogyne praetermissa, S. pusilla, S. urceolata, Scytinium fragrans, Staurothele areolata, Umbilicaria virginis, Xalocoa ocellata, Xanthoparmelia neodelisei, and the lichenicolous fungus Acarospora subfuscescens.
该研究基于对 87 份有关阿富汗报告的出版物的全面评估,以及对标本馆标本的严格修订。其中列出了 238 个地衣化真菌类群(230 个种,8 个变种)和 16 个地衣真菌种,如果是该国或各省的新记录,还列出了它们的引文或地点。阿富汗的新记录有 Acarospora bicolor、A. interrupta、Caloplaca monacensis、C. stillicidiorum var.muscorum, Collema furfuraceum, Endocarpon pusillum, Gloeoheppia turgida, Gyalolechia bracteata, Heteroplacidium fusculum, Lecanora horiza, Lecidea laboriosa, Melanohalea elegantula, Myriolecis semipallida, Peltula bolanderi, P. obscurans var.polyspora、P. radicata、Physconia americana、P. perisidiosa、Phaeophyscia hirsuta、Placidium pilosellum、P. rufescens、Sarcogyne praetermissa、S.pusilla, S. urceolata, Scytinium fragrans, Staurothele areolata, Umbilicaria virginis, Xalocoa ocellata, Xanthoparmelia neodelisei, 以及地衣真菌 Acarospora subfuscescens。
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引用次数: 0
New species, new records, and a checklist of Coenogonium (Ostropales: Coenogoniaceae) from Brazil 来自巴西的 Coenogonium(Ostropales: Coenogoniaceae)的新种、新记录和核对表
Q2 Agricultural and Biological Sciences Pub Date : 2023-12-29 DOI: 10.35535/pfsyst-2023-0027
Dayane de Oliveira Lima, Lidiane Alves dos Santos, Isaias Oliveira Junior, A. Aptroot, Robert Lücking, M. Cáceres
The genus Coenogonium, the only genus in the family Coenogoniaceae, is mostly found in tropical and subtropical regions. Approximately 100 species of Coenogonium are known worldwide, and about 52 species have been previously recorded from Brazil. Here, we describe four species as new to science from the Atlantic Forest in the states of Bahia, Minas Gerais, and Sergipe, and present a few new state records. The new species are C. carassense, C. itabaianense, C. pilosum, and C. subtomentosum. An annotated checklist of the species of Coenogonium from Brazil is also included.
Coenogonium 属是 Coenogoniaceae 科中唯一的属,主要分布在热带和亚热带地区。全世界已知的 Coenogonium 属植物约有 100 种,之前在巴西记录到的约有 52 种。在这里,我们描述了来自巴伊亚州、米纳斯吉拉斯州和塞尔希培州大西洋森林的四个新种,并介绍了一些新的州记录。这四个新种分别是 C. carassense、C. itabaianense、C. pilosum 和 C. subtomentosum。此外还包括一份巴西 Coenogonium 物种的注释核对表。
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引用次数: 0
Discovering neglected lichen diversity with DNA-based inventories: metabarcoding lichen-forming fungi in Bryce Canyon National Park, Utah, USA 利用基于 DNA 的清单发现被忽视的地衣多样性:对美国犹他州布莱斯峡谷国家公园的地衣形成真菌进行代谢标本编码
Q2 Agricultural and Biological Sciences Pub Date : 2023-12-29 DOI: 10.35535/pfsyst-2023-0007
Abigail Robinson, Pierce Adams, S. Leavitt
National parks and other federally designated natural areas play critical roles in preserving unique habitats, communities, and biodiversity. However, in the United States, it is estimated that 80–90% of species diversity in national parks is presently unknown. Therefore, contemporary biodiversity inventories are critical for conservation, management and establishing baselines for future comparisons. Ongoing efforts to characterize lichen diversity highlight diverse and robust communities in a number of national parks in the USA. In arid regions of the western USA facing ecological transformations, lichens can play a pivotal role for monitoring these changes. Lichen diversity in Bryce Canyon National Park (BRCA) in southern Utah, USA remains nearly completely uncharacterized, despite nearly 100 years as a federally protected area. Our study aims to provide a critical perspective into the lichen diversity of BRCA. Using a metabarcoding community sampling approach, we documented 215 candidate lichen-forming fungal species distributed across ecologically distinct sites in BRCA. At each sampled site, species richness ranged from 104 to 133 species, with no more than 20% shared species among the three sites. The limited overlap between collection sites suggests that BRCA harbors greater diversity than initially thought. We document a number of sensitive lichens, particularly Usnea spp. and Ramalina sinensis, that should be monitored as air pollution, land use, and impacts of climate change affect biological communities in the park. The inventory also includes unknown species and other species that have not been documented in the western USA. While our DNA-based inventory highlights strikingly rich lichen diversity, future voucher-based collections will be essential for robust taxonomic determinations.
国家公园和其他联邦指定的自然区域在保护独特的栖息地、群落和生物多样性方面发挥着至关重要的作用。然而,据估计,美国国家公园中 80-90% 的物种多样性目前尚不为人知。因此,当代生物多样性调查对于保护、管理和建立未来比较基准至关重要。目前正在进行的地衣多样性特征描述工作凸显了美国一些国家公园中多样而强大的群落。美国西部干旱地区正面临生态转型,地衣在监测这些变化方面可以发挥关键作用。尽管美国犹他州南部的布莱斯峡谷国家公园(BRCA)作为联邦保护区已有近 100 年的历史,但该公园的地衣多样性几乎完全未被描述。我们的研究旨在为布莱斯峡谷国家公园的地衣多样性提供一个重要的视角。利用代谢条码群落采样方法,我们记录了 215 个候选地衣形成真菌物种,它们分布在 BRCA 生态独特的地点。在每个采样点,物种丰富度从 104 种到 133 种不等,三个采样点之间的物种共享度不超过 20%。采集点之间有限的重叠表明,BRCA蕴藏的多样性比最初想象的要多。我们记录了一些敏感地衣,尤其是 Usnea spp.该清单还包括未知物种和其他在美国西部没有记录的物种。虽然我们基于 DNA 的清单突显了丰富的地衣多样性,但未来基于凭证的采集对于确定强大的分类学至关重要。
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引用次数: 0
Notes on the genus Usnea (lichenized Ascomycota, Parmeliaceae). V. 关于 Usnea 属(地衣化 Ascomycota,Parmeliaceae)的说明。V.
Q2 Agricultural and Biological Sciences Pub Date : 2023-12-29 DOI: 10.35535/pfsyst-2023-0018
P. Clerc, Yoshihito Ohmura
Type material of twelve species of Usnea related to Asia and South Africa were studied and one species from North America is newly described. Holotype specimens of eight Chinese taxa described in 1975 were synonymized as follows: U. mengyangensis is lectotypified and synonymized with U. aciculifera. Usnea recurvata and U. subrectangulata are synonymized with U. baileyi. Usnea kirinensis is synonymized with U. barbata s.str. Usnea crassiuscula and U. yunnanensis (= U. australis J.D. Zhao et al., nom. illeg. non Fr.) are synonymized with U. bismolliuscula. Usnea iteratocarpa is synonymized with U. cristatula (new to Asia, China). Usnea decumbens is synonymized with U. intumescens (new to Asia, China). Usnea entoviolata and U. roseola are synonymized with U. fragilis (new to Asia, China, India, Japan). The lectotype of Usnea trichoideoides was found to belong to the Usnea pectinata aggr. and consequently U. montis-fuji was resurrected for U. trichodeoides sensu Ohmura. Usnea ceratina and U. trichodeoides are excluded from the Japan lichen flora. Usnea macaronesica a recently described Macaronesian and South American species is synonymized with the Asian taxa U. pycnoclada. Usnea chicitae is described as a new species endemic of the Southern Appalachian Mountains. It is characterized by stipitate efflorescent soralia, a tenuicorticata-type of CMA and the presence of salazinic and psoromic acid in the medulla.
研究了与亚洲和南非有关的 12 种 Usnea 的模式标本,新描述了来自北美洲的 1 种。1975 年描述的 8 个中国分类群的原型标本被同化如下:mengyangensis 与 U. aciculifera 同名。Usnea recurvata 和 U. subrectangulata 与 U. baileyi 同名。Usnea kirinensis 与 U. barbata s.str.同名。Usnea crassiuscula 和 U. yunnanensis (= U. australis J.D. Zhao et al., nom. illeg. non Fr.) 与 U. bismolliuscula 同义。cristatula (new to Asia, China)同义。Usnea decumbens 与 U. intumescens(亚洲新发现,中国)同义。Usnea entoviolata 和 U. roseola 与 U. fragilis(亚洲新种,中国、印度、日本)同名。因此,U. montis-fuji 被恢复为 U. trichodeoides sensu Ohmura。trichodeoides 被排除在日本地衣植物区系之外。Usnea macaronesica 与亚洲类群 U. pycnoclada 同义。Usnea chicitae 被描述为阿巴拉契亚山脉南部特有的一个新物种。它的特征是具柄的流苏状小叶、tenuicorticata 类型的 CMA 以及髓质中含有 salazinic 和 psoromic 酸。
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引用次数: 0
The typification of Variolaria discoidea Pers. Variolaria discoidea Pers.
Q2 Agricultural and Biological Sciences Pub Date : 2023-12-29 DOI: 10.35535/pfsyst-2023-0021
Per M. Jørgensen, Imke Schmitt
The type species of the lichen genus Variolaria is V. discoidea. The name is solely based on a Hoffmann (1784) illustration. To stabilize the nomenclature, we have designated a lectotype and an epitype from Germany, for which we generated molecular data. The species was formerly placed in the genus Pertusaria as P. albescens, and is presently correctly named Lepra albescens.
地衣属 Variolaria 的模式种是 V. discoidea。该名称完全基于霍夫曼(1784 年)的一幅插图。为了稳定该命名法,我们指定了来自德国的一个标准模式和一个附生模式,并为其生成了分子数据。该物种以前被归入 Pertusaria 属,命名为 P. albescens,目前正确的命名为 Lepra albescens。
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引用次数: 0
A new species of Agonimia (Ascomycota, Eurotiomycetes, Verrucariaceae) from Morane atoll (Tuāmotu-Gambier Islands, French Polynesia) 来自莫拉内环礁(法属波利尼西亚图阿莫图-甘比尔群岛)的 Agonimia(子囊菌目,欧洲真菌纲,疣柄菌科)新种
Q2 Agricultural and Biological Sciences Pub Date : 2023-12-29 DOI: 10.35535/pfsyst-2023-0023
R. Poncet, Jean-Yves Meyer, Anne-Hélène Paradis, Sébastien Leblond, Lionel Kervran
Agonimia gargominyi (Verrucariaceae, Verrucariales) is described and illustrated from bark samples collected from Morane atoll (Tuāmotu-Gambier Islands, French Polynesia). It is characterized by the large superficial perithecia having a pale brown-colored ostiolar region, the 8-spored asci, and the relatively large lobed squamules. Micromorphology of the ascomata and the thalline squamules matches the genus Agonimia. Additionally, to accommodate the inclusion of this newly identified species, the existing comprehensive key encompassing all known Agonimia species worldwide has been modified and updated accordingly. Detailed illustrations supplement the description of Agonimia gargominyi.
本文描述并展示了从莫拉内环礁(法属波利尼西亚图阿莫图-甘比尔群岛)采集的树皮样本中发现的 Agonimia gargominyi(疣癣菌科,Verrucariaceae, Verrucariales)。它的特征是具有淡褐色外壳区的大型表层珠孔、8 个孢子囊和相对较大的浅裂鳞片。菌丝体和叶状鳞片的微观形态与 Agonimia 属相符。此外,为了将这一新鉴定的物种纳入其中,对包含全球所有已知 Agonimia 物种的现有综合检索表进行了相应的修改和更新。详细的插图对 Agonimia gargominyi 的描述进行了补充。
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引用次数: 0
Thalloidima squamatum comb. nov. - a distinct and common but overlooked lichen in Europe Thalloidima squamatum comb.
Q2 Agricultural and Biological Sciences Pub Date : 2023-12-29 DOI: 10.35535/pfsyst-2023-0019
M. Westberg, S. Ekman, Laura Briegel-Williams, S. Fernández-Brime, M. Wedin, E. Timdal
The new combination Thalloidima squamatum is proposed for a morphologically and phylogenetically distinct species, until now included within the variation of T. sedifolium. Compared to T. sedifolium, the species is characterized by its larger squamules and apothecia, shorter ascospores, the pale color of the excipulum, and its habitat growing on moss cushions over calcareous rock or directly on rock rather than on soil. Most of the material studied originates from Norway and Sweden, but specimens are also reported from Austria, Croatia, Germany, Greenland, Italy, Poland, Russia, Scotland and Switzerland.
新的组合Thalloidima squamatum是为一个形态上和系统发育上独特的物种而提出的,直到现在该物种还被归入T. sedifolium的变种中。与 T. sedifolium 相比,该物种的特点是鳞片和皮孔较大,无托叶较短,外生孢子颜色较浅,栖息地生长在石灰质岩石上的苔藓垫上或直接生长在岩石上而非土壤中。研究的大部分材料来自挪威和瑞典,但也有来自奥地利、克罗地亚、德国、格陵兰岛、意大利、波兰、俄罗斯、苏格兰和瑞士的标本报道。
{"title":"Thalloidima squamatum comb. nov. - a distinct and common but overlooked lichen in Europe","authors":"M. Westberg, S. Ekman, Laura Briegel-Williams, S. Fernández-Brime, M. Wedin, E. Timdal","doi":"10.35535/pfsyst-2023-0019","DOIUrl":"https://doi.org/10.35535/pfsyst-2023-0019","url":null,"abstract":"The new combination Thalloidima squamatum is proposed for a morphologically and phylogenetically distinct species, until now included within the variation of T. sedifolium. Compared to T. sedifolium, the species is characterized by its larger squamules and apothecia, shorter ascospores, the pale color of the excipulum, and its habitat growing on moss cushions over calcareous rock or directly on rock rather than on soil. Most of the material studied originates from Norway and Sweden, but specimens are also reported from Austria, Croatia, Germany, Greenland, Italy, Poland, Russia, Scotland and Switzerland.","PeriodicalId":52151,"journal":{"name":"Plant and Fungal Systematics","volume":" 10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139144265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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