Infection Genetics and Evolution最新文献

{"title":"High detection rate and genetic diversity of feline caliciviruses in an island population of feral cats with/without oral lesions","authors":"Jessica L. Kulberg ,&nbsp;Felix N. Toka ,&nbsp;Yashpal S. Malik ,&nbsp;Souvik Ghosh","doi":"10.1016/j.meegid.2026.105892","DOIUrl":"10.1016/j.meegid.2026.105892","url":null,"abstract":"<div><div>Although feline caliciviruses (FCVs) (Family <em>Caliciviridae</em>) have been studied extensively in household and shelter cats, limited information is available on FCVs in feral cat (<em>Felis catus</em>) populations. We report here high FCV detection rates (27.77%, 30/108) in oral samples from feral cats on the Caribbean island of St. Kitts. Fourteen (46.66%) of the FCV positive cats exhibited oral lesions that were characteristic of FCV infection. Based on analysis of VP1 sequences, the FCV strains from St. Kitts (FCP strains) formed two phylogenetically distinct clusters within FCV genotype-I, and exhibited significant genetic diversity between themselves, and with those of other FCVs (including the FCV vaccine strains). We identified genetically distinct FCV variants co-circulating in certain feral cat colonies, and hypothesized multiple-independent introductions of FCVs into the island feline population (especially from North America). Based on analysis of the putative VP1 E region, the FCP strains retained the conserved amino acid (aa) residues that are crucial for FCV binding to host receptor, whilst aa mismatches at the neutralizing and non-neutralizing epitopes, and at the virulence-related aa positions were observed between the FCP strains (and between the FCP strains and FCV vaccine strains). Taken together, our findings highlighted the complex molecular epidemiology of FCVs in the St. Kitts feral cat population, which might facilitate emergence of virulent pathotypes and/or antigenic variants. Considering these observations, and the potential risk of FCV transmission between feral and household cats, large-scale molecular epidemiological studies on FCVs in feral cat populations from different geographical regions are of utmost importance. To our knowledge, this is the first report on molecular prevalence and genetic diversity of FCVs from the Caribbean region.</div></div>","PeriodicalId":54986,"journal":{"name":"Infection Genetics and Evolution","volume":"138 ","pages":"Article 105892"},"PeriodicalIF":2.6,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146133639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptome integration analysis of shared biomarkers and common immune mechanisms in SLE and PSO","authors":"Meijia Cheng ,&nbsp;Yue Pei ,&nbsp;Baoyue Li ,&nbsp;Yanhui Yu ,&nbsp;Jiangning Li ,&nbsp;Jingyan Zhang ,&nbsp;Xiaodong Sun ,&nbsp;Dan Zou ,&nbsp;Yunen Liu ,&nbsp;Yichen Wang","doi":"10.1016/j.meegid.2026.105886","DOIUrl":"10.1016/j.meegid.2026.105886","url":null,"abstract":"<div><div>This study aimed to identify shared diagnostic biomarkers and common immune mechanisms between systemic lupus erythematosus (SLE) and psoriasis (PSO) via integrated transcriptomic analysis, and to elucidate the role of genetic susceptibility in driving disease pathogenesis following viral infection. GEO datasets of SLE and PSO were analyzed. Shared genes were screened using differential expression analysis and WGCNA. 92 DEGs were identified, and 7 key shared genes (<em>OASL, SAMD9, IFI6, OAS3, NMI, UBE2L6, MX1</em>) were determined after WGCNA and intersection analysis. Among 8 machine learning models, LASSO performed best: for SLE, training set AUC was 0.935, external validation AUCs were 0.764 (accuracy 0.745) and 0.844 (accuracy 0.896); for PSO, training set AUC was 0.841, internal validation AUC 0.910 (accuracy 0.989), external validation AUCs 0.941 (accuracy 0.968) and 0.869 (accuracy 0.815). Immune infiltration analysis showed significant correlations between key genes and specific immune cell subsets.Immunofluorescence analysis confirmed elevated protein expression levels of UBE2L6 and SAMD9 in both diseases.Single-cell analysis revealed that most key genes were differentially expressed in dendritic cells and monocytes in SLE, but in T cells in PSO. SLE and PSO share 7 susceptibility genes that are significantly enriched in immune response pathways related to viral infections. The genetic susceptibility of these genes can lead to imbalance or excessive activation of the body's antiviral defense, and through dysregulation of innate and adaptive immunity, promote the occurrence and development of the diseases. The LASSO model further supports the reliability of these genes as potential diagnostic biomarkers for SLE and PSO.</div></div>","PeriodicalId":54986,"journal":{"name":"Infection Genetics and Evolution","volume":"138 ","pages":"Article 105886"},"PeriodicalIF":2.6,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146025080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of non-destructive DNA extraction for the molecular and morphological identification of tick species and their pathogens","authors":"Camille Lorang ,&nbsp;Clémence Galon ,&nbsp;Olivier Plantard ,&nbsp;Denis Augot","doi":"10.1016/j.meegid.2026.105880","DOIUrl":"10.1016/j.meegid.2026.105880","url":null,"abstract":"<div><div>Tick-borne diseases remain a major concern in both human and animal health in most biogeographical regions. Since certain tick-borne pathogens are transmitted by specific tick species, tick identification through morphological and biomolecular examination of the species is highly recommended for investigation of tick-borne diseases. In this study, we assessed a non-destructive DNA protocol for identifying tick species and screening of pathogens and evaluate the effects of this protocol on the tick body, using geometric morphometric (based on coxa 1). Overall, 80 <em>Ixodes</em> spp. specimens (<em>I. ricinus</em>, <em>I. hexagonus</em>, and <em>I. acuminatus</em>) conserved in alcohol for more than 15 years were tested here, including all stages (male, female, nymph and larva). Molecular investigation using 16S rRNA enabled 92% of ticks to be assigned to a species. The microfluidic chip demonstrates the presence of <em>Bartonella</em> sp. (13%), <em>Rickettsia helvetica</em> (63%) and <em>Hepatozoon</em> sp. (13%) in tested engorged females. Comparison of the coxa 1 shape before and after extraction showed no changes in morphology. We demonstrated that DNA can be extracted from old specimens of hard ticks using non-destructive methods, allowing for molecular identification of ticks and pathogens without altering their morphology. As a result, this technique makes it possible to preserve specimens from laboratory or museum collections. Additionally, non-destructive DNA extraction could be useful in medical entomology for monitoring arrivals of alien species and emergence of associated tick-borne diseases affecting humans, domestic animals, or wildlife.</div></div>","PeriodicalId":54986,"journal":{"name":"Infection Genetics and Evolution","volume":"138 ","pages":"Article 105880"},"PeriodicalIF":2.6,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145981925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional annotation of HBV-associated host genetic variants in the Saudi population: A bioinformatic analysis for precision hepatitis B research","authors":"Saira Sarfraz Khalid ,&nbsp;Khalid Alswat","doi":"10.1016/j.meegid.2026.105878","DOIUrl":"10.1016/j.meegid.2026.105878","url":null,"abstract":"<div><div>Chronic hepatitis B virus (HBV) infection poses a global public health challenge, for which an effective cure remains elusive. A substantial amount of data has shown that single nucleotide polymorphisms (SNPs) within host genes can affect the regulation and expression of proteins, thereby influencing the susceptibility to HBV infection as well as disease progression and response to treatment. HBV-related SNPs have been identified in the population of the Kingdom of Saudi Arabia (KSA), however, there is a lack of in-depth characterization of the translational and functional impact of these SNPs. This narrative review summarizes and functionally contextualizes the twenty-three reported SNPs across ten genes that showed significant association with HBV-related complications within the Saudi population. These genetic variants are primarily involved in immune signaling, interferon response, and inflammatory regulation pathways, indicating their potential influence on host-virus interactions and disease progression. Using bioinformatic prediction tools, their possible functional impacts were evaluated, providing insights into their biological relevance. The review also highlights future research directions for HBV genomics in Saudi Arabia. Collectively, these findings could contribute to the development of more effective preventive and therapeutic strategies through personalized management of HBV infection.</div></div>","PeriodicalId":54986,"journal":{"name":"Infection Genetics and Evolution","volume":"138 ","pages":"Article 105878"},"PeriodicalIF":2.6,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146020871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Population structure of G. pallidipes tsetse flies in Northwestern Kenya - Towards effective vector control","authors":"Winnie A. Okeyo ,&nbsp;Sylvia Korir ,&nbsp;Daniel Ochieng’ Gamba ,&nbsp;Miriam Jemutai ,&nbsp;Samuel Onyoyo Guya ,&nbsp;David N. Ndung'u ,&nbsp;Paul O. Mireji ,&nbsp;Rosemary Bateta","doi":"10.1016/j.meegid.2026.105881","DOIUrl":"10.1016/j.meegid.2026.105881","url":null,"abstract":"<div><h3>Background</h3><div>Tsetse flies are vectors of trypanosome parasites responsible for Human and Animal African Trypanosomiasis (HAT and AAT), diseases that greatly affect health and economic growth across sub-Saharan Africa. Vector control, one of the possible strategies to tackle these diseases, is most effective on genetically isolated tsetse populations. While the population genetic structure of <em>Glossina pallidipes</em> tsetse flies has been well studied in southern Kenya, little is known about populations in northwestern Kenya. This study investigated the presence, genetic diversity, and population structure of <em>G. pallidipes</em> in Turkana County in northwestern Kenya and surrounding regions.</div></div><div><h3>Methods</h3><div>Tsetse fly surveys were carried out in Turkana, West Pokot, Samburu and Baringo Counties in northwestern Kenya. DNA was extracted from the legs of flies for genotyping using 11 microsatellite markers for <em>G. pallidipes</em>, followed by analysis using various population genetics software to evaluate the genetic diversity and population structure. DNA from gut and mouthparts was screened for trypanosomes.</div></div><div><h3>Results and discussion</h3><div><em>G. pallidipes</em> were only detected in Turkana despite similar ecological conditions in adjacent counties, which is mostly semi-arid. This suggests that anthropogenic factors like habitat clearing and vector control may limit their distribution. Genetic analysis revealed high genetic diversity in Turkana populations, similar to those from the previously described cluster in eastern Kenya, indicating demographic stability in this ecosystem. Clustering analysis showed that while most Turkana samples grouped with eastern Kenyan populations, those from Northern Turkana (Oropoi) formed a distinct cluster, pointing to possible genetic isolation. Trypanosome screening identified <em>T. vivax</em> in 3.3% of the samples, though not indicating active transmission.</div></div><div><h3>Recommendations</h3><div>The possibly genetically distinct tsetse population in Oropoi suggests limited gene flow, possibly due to geographic or ecological barriers. Further studies with expanded sampling are needed to confirm these findings and assess the boundaries of this genetic cluster beyond the Kenya-Uganda border. Integrating genetic data into surveillance and control programs will enhance the effectiveness of tsetse management strategies.</div></div>","PeriodicalId":54986,"journal":{"name":"Infection Genetics and Evolution","volume":"138 ","pages":"Article 105881"},"PeriodicalIF":2.6,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145991854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic insights into Mycobacterium orygis in wild ungulates in Chennai, India","authors":"Harini Ramanujam ,&nbsp;Mahaprabhu Ramalingam ,&nbsp;Ahmed Kabir Refaya ,&nbsp;Priya Rajendran ,&nbsp;M. Baskar ,&nbsp;Natesan Palanivel ,&nbsp;Saraswathi Devarajan ,&nbsp;Angayarkanni Balasubramaniam ,&nbsp;R. Nithya ,&nbsp;Sivakumar Shanmugam ,&nbsp;Kannan Palaniyandi","doi":"10.1016/j.meegid.2025.105869","DOIUrl":"10.1016/j.meegid.2025.105869","url":null,"abstract":"<div><div>Bovine tuberculosis (bTB) represents a significant global threat to livestock, wildlife, and human health, yet wildlife tuberculosis (wTB) remains underreported in India. This study aimed to investigate the presence of <em>Mycobacterium tuberculosis</em> complex (MTBC) species in wild ungulates at Guindy National Park, Chennai. Postmortem tissue samples and fecal pellets were collected from spotted deer, sambar deer, and blackbuck, and analyzed using culture, molecular diagnostics, histopathology, and whole genome sequencing (WGS). <em>Mycobacterium orygis</em> was isolated from tissue samples of four animals, while molecular assays detected MTBC DNA in nine fecal samples. Drug resistance was identified in three fecal samples. Histopathology revealed characteristic granulomatous lesions, and WGS confirmed <em>M. orygis</em> in all tissue-derived isolates. Pangenome analysis identified 4222 genes with an estimated γ value of 0.0086, suggesting an open pangenome with novel genes accumulating at a slow rate. Comparative genomic analysis, including insertion sequence (IS) profiling and SNP analysis, revealed limited overall diversity but unique SNPs in Chennai isolates, suggesting local genomic differentiation. These findings highlight the dynamic nature of <em>M. orygis</em> within MTBC and highlight the importance of functional studies to understand host specificity and adaptability and the urgent need for systematic wTB surveillance and One Health-based interventions in India.</div></div>","PeriodicalId":54986,"journal":{"name":"Infection Genetics and Evolution","volume":"137 ","pages":"Article 105869"},"PeriodicalIF":2.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145806429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic dynamics of the Duffy antigen receptor for chemokines gene and Plasmodium vivax circulation within sub-Saharan Africa","authors":"Favour Adeloye ,&nbsp;Kaothar O. Lambe ,&nbsp;Jennifer A. Oboh ,&nbsp;Halima Abdulsalam ,&nbsp;Chinatu Enyinnnaya ,&nbsp;Roseangela Nwuba ,&nbsp;Olumide Ogundahunsi ,&nbsp;Umberto D'Alessandro ,&nbsp;Alfred Amambua-Ngwa ,&nbsp;Martin M. Meremikwu ,&nbsp;Grant Hughes ,&nbsp;Eva Heinz ,&nbsp;Mary A. Oboh","doi":"10.1016/j.meegid.2025.105863","DOIUrl":"10.1016/j.meegid.2025.105863","url":null,"abstract":"<div><h3>Introduction</h3><div>Duffy antigen receptor for chemokines (DARC) is a transmembrane receptor (glycoprotein) expressed on human red blood cells. Sub-Saharan Africa (sSA) individuals, who suffer the most of the global malaria burden, predominantly carry a Duffy negative phenotype. Expression of this gene (found among Duffy-positive individuals) is known to be essential for <em>P. vivax</em> invasion of RBCs. While <em>P. falciparum</em> is the predominant <em>Plasmodium</em> in sSA, the upward trend in <em>P. vivax</em> infection is a major threat to the malaria eradication programme in the region. Since Duffy null individuals (homozygous negative) lack DARC expression, we investigated the DARC gene dynamics in relation to the emerging presence of <em>P. vivax</em> infections in a previously predominant <em>P. falciparum</em> endemic region.</div></div><div><h3>Methods</h3><div>A total of 223 DARC genes were retrieved from the NCBI database across various countries, Nigeria, Cameroon, Ethiopia, Madagascar and South Africa and were used for population dynamic analysis using different population genetic metrics.</div></div><div><h3>Findings</h3><div>Among these sSA countries, South Africa showed the most haplotype and nucleotide diversity compared to other parts of sSA. Various selection pressures were observed in Western Africa and the Central African Republic. Population structure analysis revealed DARC population clustering of Cameroon, Nigeria and Ethiopia (despite Ethiopia's geographic distance), suggestive of shared ancestry and minimal DARC locus divergence. Conversely, South Africa and Madagascar showed a distinct genetic lineage reflecting differences in evolutionary pressures.</div></div><div><h3>Conclusion</h3><div>Our analysis suggests minimal genetic diversity within sSA with evidence of selection potentially attributed to the recent emergence of <em>P. vivax</em> infections. However, greater diversity was observed in South Africa. Evidence of selection of this gene and detection of <em>P. vivax</em> among Duffy-null individuals in the other regions is truly a public health concern.</div></div>","PeriodicalId":54986,"journal":{"name":"Infection Genetics and Evolution","volume":"137 ","pages":"Article 105863"},"PeriodicalIF":2.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145727241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gaining insights into novel pathogen hosts: Characterisation of Actinomyces weissii strains isolated from companion animals","authors":"Beyza Cirak ,&nbsp;Antonia Kreitlow ,&nbsp;Maria Borowiak ,&nbsp;Christiane Hoffmann ,&nbsp;Christoph Lämmler ,&nbsp;Carsten Heydel ,&nbsp;Christa Ewers ,&nbsp;Burkhard Malorny ,&nbsp;Madeleine Plötz ,&nbsp;Amir Abdulmawjood","doi":"10.1016/j.meegid.2025.105867","DOIUrl":"10.1016/j.meegid.2025.105867","url":null,"abstract":"<div><div><em>Actinomyces weissii</em> was first isolated from the oral cavity of dogs in 2012. This study characterised the phenotypic and genotypic features of 11 additional strains obtained from diverse pathological lesions in companion animals. A multi-faceted approach was employed, combining culture techniques, biochemical profiling, MALDI-TOF MS, real-time PCR, sequencing of genetic markers, and whole-genome analysis. Despite minor phenotypic variation, MALDI-TOF MS and genotypic analyses consistently confirmed species identity and revealed intraspecies diversity. Whole-genome sequencing revealed four haemolysin family protein genes, and all isolates exhibited complete β-haemolysis. Pan-genome analysis defined a conserved core genome of 1559 genes, shared by all isolates and the reference strain, including haemolysin-related genes. Phylogenetic comparisons placed <em>A. urogenitalis</em> and <em>A. trachealis</em> as closest relatives.</div><div>These findings broaden the host spectrum of <em>A. weissii</em>, with the first isolation from a cat, suggesting that this species may also occur in felines and merits consideration in veterinary diagnostics. Its presence in companion animals raises the possibility of zoonotic risk, and together with the detection of haemolysin family protein genes, underscores the need for further investigation to clarify its pathogenic significance and potential impact on veterinary practice and public health.</div></div>","PeriodicalId":54986,"journal":{"name":"Infection Genetics and Evolution","volume":"137 ","pages":"Article 105867"},"PeriodicalIF":2.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145795587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic diversity and evolutionary relationships of porcine astroviruses from wild boars and domestic pigs in Japan","authors":"Shuntaro Mizuno ,&nbsp;Natsuko Teshima ,&nbsp;Tomoko Yokota ,&nbsp;Shwe Thiri Maung Maung Khin ,&nbsp;Hitoshi Takemae ,&nbsp;Toru Oi ,&nbsp;Fujiko Fukuda ,&nbsp;Tsuneyuki Masuda ,&nbsp;Yasuhiro Kikkawa ,&nbsp;Takanori Shiga ,&nbsp;Naoyuki Aihara ,&nbsp;Junichi Kamiie ,&nbsp;Hiroho Ishida ,&nbsp;Hironobu Murakami ,&nbsp;Tetsuya Mizutani ,&nbsp;Mami Oba ,&nbsp;Makoto Nagai","doi":"10.1016/j.meegid.2025.105875","DOIUrl":"10.1016/j.meegid.2025.105875","url":null,"abstract":"<div><div>Porcine astrovirus (PoAstV) is a viral pathogen associated with diarrhea and neurological disorders in piglets. In this study, we performed metatranscriptome analysis of fecal samples, and compared the full-length genomes of PoAstVs detected in Japanese wild boars and domestic pigs collected between 2017 and 2023 with astrovirus sequences from the GenBank database. Phylogenetic analyses of near-complete genomes from 12 wild boar and 67 pig PoAstVs, together with sequences from our previous study and the GenBank database, revealed that six, four, and two wild boar PoAstV sequences clustered with PoAstV-2, PoAstV-4, and PoAstV-5, respectively. Pig-derived PoAstVs comprised all five known genotypes, with PoAstV-2 (31/67) being predominant, followed by PoAstV-4 (18/67) and PoAstV-5 (12/67), similar to the pattern observed in wild boars. Some wild boar PoAstV strains showed high nucleotide identity with domestic Japanese strains, whereas others exhibited greater similarity to foreign porcine strains. Notably, two wild boar PoAstV-4 strains showed high identity with Chinese and Vietnamese PoAstV-4 in ORF1a and ORF1b, forming a distinct cluster, while no clustering was observed in ORF2, indicating possible recombination events. Intragenotype recombination was also detected among wild boar and domestic pig strains of PoAstV-2, −4, and − 5, implying wildlife-domestic interface. These findings suggest that PoAstVs are exchanged between wild boars and domestic pigs in Japan, promoting genetic diversification within each genotype through recombination. To our knowledge, this is the first detection and genomic characterization of PoAstV in Japanese wild boars.</div></div>","PeriodicalId":54986,"journal":{"name":"Infection Genetics and Evolution","volume":"137 ","pages":"Article 105875"},"PeriodicalIF":2.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145893037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Distribution of voltage-gated sodium channel and acetylcholinesterase mutations in Bemisia tabaci species from the Democratic Republic of the Congo","authors":"Olivier Likiti Kola ,&nbsp;Christophe Simiand ,&nbsp;Hadija Mussa Ally ,&nbsp;Daniel Otron ,&nbsp;Sharon L. van Brunschot ,&nbsp;Justin S. Pita ,&nbsp;John Colvin ,&nbsp;Godefroid Monde Te-Kazangba ,&nbsp;Hélène Delatte","doi":"10.1016/j.meegid.2025.105866","DOIUrl":"10.1016/j.meegid.2025.105866","url":null,"abstract":"<div><div>The intensive use of insecticides can result in the emergence of resistant genotypes, as observed globally in the <em>B. tabaci</em> species complex. Two well-studied/known cases of resistance mechanisms in <em>B. tabaci</em> involve mutations in the voltage-gated sodium channel (Kdr) and acetylcholinesterase (Ace 1) genes. However, no studies have surveyed these resistance mechanisms in whitefly populations from Central Africa. To study the frequency of these mutations in whitefly species on cassava, 1315 individuals were collected from 60 fields across 12 provinces of the Democratic Republic of the Congo. Species were identified molecularly, and then the frequencies of Kdr and Ace 1 mutations were assessed. Four species were found: SSA1-SG1 ∪ SG2 (71.48 %); SSA1-SG3 (0.31 %); SSA2 ∪ SSA3 (15.51 %), and MED ASL (12.70 %). For the Kdr mutation, 9 % of individuals of SSA1-SG1 ∪ SG2, 6 % of MED ASL, and 3 % of SSA2USSA3 carried at least one resistant allele. For the Ace 1 mutation, resistance alleles were found in 0.1 % of SSA1-SG1 ∪ SG2, 6 % of MED ASL, and 1 % of SSA2 ∪ SSA3 individuals. The geographical distribution of resistant genotypes remains limited to certain regions. However, there is a long-term risk that resistance becomes established around areas where insecticides are used on cotton and vegetable crops. Appropriate strategies for the management of these vectors of phytoviruses are discussed.</div></div>","PeriodicalId":54986,"journal":{"name":"Infection Genetics and Evolution","volume":"137 ","pages":"Article 105866"},"PeriodicalIF":2.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145795578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}