To evaluate the antidepressant-like effect of pearl powder in rats and explore its potential mechanism based on network pharmacology analysis and experimental validation.The chronic multiple mild stress (CMMS) rat model was applied to evaluate the effects of pearl powder on depression-like behaviors in rats through the sucrose preference test, forced swimming test, and tail suspension test.In addition, based on the identification of the major amino acid components of pearl powder, a network pharmacology analysis was performed to identify potential molecular targets of pearl powder.Subsequently, we compared the identified targets of pearl powder with depression-related targets by a Venn diagram and obtained the intersecting key genes.These genes were further used to construct PPI network and KEGG pathway analysis.Finally, Western blotting assay was applied to verify the protein levels of the relevant targets regulated by pearl powder to explore the underlying mechanisms.The behavioral tests showed that different doses of pearl powder (16.7 mg/kg, 83.3 mg/kg, and 166.7 mg/kg, administered once daily for one week by gavage) significantly improved the sucrose preference and alleviated depression-like behaviors in CMMS rats (FST: immobility time decreased by 12.34% 2.34%; TST: immobility time decreased by 12.03% 7.75%).After pretreatment of pearl powder with hydrochloric acid, the major amino acids in pearl powder were detected by an automatic amino acid analyzer, with top 5 (Gly, Ala, Glu, Asp, and Arg) accounting for more than 80% of the total amount.Network pharmacology analysis revealed that the top 5 active amino acids in pearl powder could regulate several targets such as CASP3, BDNF, MTOR, GluN2B, and MAPK1.The 84 key genes were also obtained after compared with depression-related target genes.In the following GO enrichment and KEGG pathway analysis, the top 10 items in the bubble plot indicated that pearl powder might exert anti-depression effects by regulating neuronal synaptic membranes and signal transmission.The next Western blot analysis verified that pearl powder could upregulate GluN2Aand synaptic-related proteins PSD-95 and BDNF, thereby improving the synaptic structure and function in the hippocampus.This study first clearly demonstrated that pearl powder significantly improved depressive-like behavior in CMMS rats.By combining network pharmacology and confirmatory experiments, it elucidated that the major active amino acids in pearl powder might participate in the repair of synaptic damage by regulating the expression of synaptic-related proteins, ultimately exerting its pharmacological activity against depression.
{"title":"Pearl Powder Exerts Antidepressant Effects by Modulating the GluN2A/BDNF/PSD-95 Signaling Axis","authors":"Hao Liu, Haohui Sun, Yizhou Zhang, Huijun Zheng, Feng Gao","doi":"10.25135/rnp.551.2506.3569","DOIUrl":"https://doi.org/10.25135/rnp.551.2506.3569","url":null,"abstract":"To evaluate the antidepressant-like effect of pearl powder in rats and explore its potential mechanism based on network pharmacology analysis and experimental validation.The chronic multiple mild stress (CMMS) rat model was applied to evaluate the effects of pearl powder on depression-like behaviors in rats through the sucrose preference test, forced swimming test, and tail suspension test.In addition, based on the identification of the major amino acid components of pearl powder, a network pharmacology analysis was performed to identify potential molecular targets of pearl powder.Subsequently, we compared the identified targets of pearl powder with depression-related targets by a Venn diagram and obtained the intersecting key genes.These genes were further used to construct PPI network and KEGG pathway analysis.Finally, Western blotting assay was applied to verify the protein levels of the relevant targets regulated by pearl powder to explore the underlying mechanisms.The behavioral tests showed that different doses of pearl powder (16.7 mg/kg, 83.3 mg/kg, and 166.7 mg/kg, administered once daily for one week by gavage) significantly improved the sucrose preference and alleviated depression-like behaviors in CMMS rats (FST: immobility time decreased by 12.34% 2.34%; TST: immobility time decreased by 12.03% 7.75%).After pretreatment of pearl powder with hydrochloric acid, the major amino acids in pearl powder were detected by an automatic amino acid analyzer, with top 5 (Gly, Ala, Glu, Asp, and Arg) accounting for more than 80% of the total amount.Network pharmacology analysis revealed that the top 5 active amino acids in pearl powder could regulate several targets such as CASP3, BDNF, MTOR, GluN2B, and MAPK1.The 84 key genes were also obtained after compared with depression-related target genes.In the following GO enrichment and KEGG pathway analysis, the top 10 items in the bubble plot indicated that pearl powder might exert anti-depression effects by regulating neuronal synaptic membranes and signal transmission.The next Western blot analysis verified that pearl powder could upregulate GluN2Aand synaptic-related proteins PSD-95 and BDNF, thereby improving the synaptic structure and function in the hippocampus.This study first clearly demonstrated that pearl powder significantly improved depressive-like behavior in CMMS rats.By combining network pharmacology and confirmatory experiments, it elucidated that the major active amino acids in pearl powder might participate in the repair of synaptic damage by regulating the expression of synaptic-related proteins, ultimately exerting its pharmacological activity against depression.","PeriodicalId":21053,"journal":{"name":"Records of Natural Products","volume":"1 6","pages":"702-716"},"PeriodicalIF":0.0,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://doi.org/10.25135/rnp.551.2506.3569","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147917657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-09-07DOI: 10.25135/rnp.545.2507.3597
Damla Kırcı, Nurhayat Tabanca, Eugene K. Blythe, Betül Demırcı
This study investigates the phytochemical variations among 34 cultivars of Ocimum basilicum to evaluate their essential oil composition and identify chemotaxonomic patterns.Understanding this variation is important for cultivar authentication, selection, and potential industrial or pharmacological applications.Essential oils were extracted via hydrodistillation and analyzed using gas chromatography-mass spectrometry (GC/MS).To assess chemical variation and group cultivars based on their volatile profiles, principal component analysis (PCA) and hierarchical cluster analysis (HCA) were applied.A total of 48 volatile compounds were identified across the cultivars.Linalool was the most abundant constituent, dominating in 28 cultivars with concentrations ranging from 24.2% to 81.1%.Methyl chavicol exhibited strikingly high levels in certain cultivars, reaching up to 82.0% in some samples.Cluster analysis revealed two major chemotype clades, with chemical similarity indices ranging from -33.07% to 90.84%, indicating substantial intra-specific variation and the potential for clear chemotaxonomic discrimination.The essential oil composition of O. basilicum cultivars exhibited marked variability, supporting classification based on chemotypic profiles.These findings enhance the understanding of chemotaxonomy within the genus and provide practical insights for cultivar selection in medicinal, aromatic, and commercial applications.
{"title":"Phytochemical Variations in Ocimum basilicum L. Cultivars: Essential Oil Composition and Multivariate Chemotype Differentiation","authors":"Damla Kırcı, Nurhayat Tabanca, Eugene K. Blythe, Betül Demırcı","doi":"10.25135/rnp.545.2507.3597","DOIUrl":"https://doi.org/10.25135/rnp.545.2507.3597","url":null,"abstract":"This study investigates the phytochemical variations among 34 cultivars of Ocimum basilicum to evaluate their essential oil composition and identify chemotaxonomic patterns.Understanding this variation is important for cultivar authentication, selection, and potential industrial or pharmacological applications.Essential oils were extracted via hydrodistillation and analyzed using gas chromatography-mass spectrometry (GC/MS).To assess chemical variation and group cultivars based on their volatile profiles, principal component analysis (PCA) and hierarchical cluster analysis (HCA) were applied.A total of 48 volatile compounds were identified across the cultivars.Linalool was the most abundant constituent, dominating in 28 cultivars with concentrations ranging from 24.2% to 81.1%.Methyl chavicol exhibited strikingly high levels in certain cultivars, reaching up to 82.0% in some samples.Cluster analysis revealed two major chemotype clades, with chemical similarity indices ranging from -33.07% to 90.84%, indicating substantial intra-specific variation and the potential for clear chemotaxonomic discrimination.The essential oil composition of O. basilicum cultivars exhibited marked variability, supporting classification based on chemotypic profiles.These findings enhance the understanding of chemotaxonomy within the genus and provide practical insights for cultivar selection in medicinal, aromatic, and commercial applications.","PeriodicalId":21053,"journal":{"name":"Records of Natural Products","volume":"1 6","pages":"688-701"},"PeriodicalIF":0.0,"publicationDate":"2025-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://doi.org/10.25135/rnp.545.2507.3597","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147911920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Three crude polysaccharides of RCP-A, B, and C were derived from the rhizome of Rohdea chinensis by means of hot water extraction, gradient ethanol precipitation and dialysis.Three different polysaccharides of RCP-C1-1, RCP-C1-2, and RCP-C1-3 were isolated using cellulose DEAE-52 and Sephadex G-200 chromatography from RCP-C.The average molecular weights of RCP-C1-1, RCP-C1-2, and RCP-C1-3 were measured as 1.5110 4 , 1.0610 4 , and 4.8610 3 by means of MALDI-TOF MS and UHPGC, respectively.All three polysaccharides were found to consist of D-fructose and D-glucose following hydrolysis and comparison with literature data.Based on FT-IR and NMR analysis, the polysaccharides were identified as inulin-type fructans, with their backbone composed of -D-glucopyranosyl-(12)-(-D-fructofuranosyl)n-(12)--Dfructofuranoside (nRCP-C1-1=79, nRCP-C1-2=58, nRCP-C1-3=27).The anti-tumor activity of the polysaccharides (RCP-A, B, and C) was evaluated in H22 tumor-bearing mice.The results suggested that the polysaccharides (RCP-A, RCP-B, and RCP-C) inhibited the growth of H22 hepatocellular.Further, the treated groups of RCP-A, RCP-B, and RCP-C exhibited improvements in body weight as well as spleen/thymus indexes in H22 tumor-bearing mice.
采用热水提取、梯度乙醇沉淀和透析等方法，从中药根茎中分离得到RCP-A、B和C三种粗多糖。采用DEAE-52纤维素层析和Sephadex G-200层析分离RCP-C1-1、RCP-C1-2和RCP-C1-3三种不同的多糖。采用MALDI-TOF质谱法和UHPGC法测得RCP-C1-1、RCP-C1-2和RCP-C1-3的平均分子量分别为1.5110 4、1.0610 4和4.8610 3。这三种多糖均由d -果糖和d -葡萄糖组成，并与文献数据进行了比较。通过FT-IR和NMR分析，鉴定该多糖为菊糖型果聚糖，其骨架结构为- d -葡萄糖吡喃基-(12)-（- d -聚呋喃基）n-(12)-聚呋喃苷（nRCP-C1-1=79, nRCP-C1-2=58, nRCP-C1-3=27）。对RCP-A、B、C三种多糖在H22荷瘤小鼠体内的抗肿瘤活性进行了评价。结果表明，多糖（RCP-A、RCP-B和RCP-C）对H22肝细胞的生长有抑制作用。此外，RCP-A、RCP-B和RCP-C处理组对H22荷瘤小鼠的体重和脾脏/胸腺指数均有改善。
{"title":"Structure Characterization and Anti-tumor Activity of Polysaccharides from Rohdea chinensis","authors":"Shihao Zhang, Sicheng Liu, Minghua Ma, Cheng‐Xiong Liu, Kun Zou, Wencong Li, Yanyu Li, Yu Zhao, Xueyan Li, Guoce Jin, Zixuan Zhu, Zhaoxia Liu, Xiaocong Li","doi":"10.25135/rnp.454.2403.3165","DOIUrl":"https://doi.org/10.25135/rnp.454.2403.3165","url":null,"abstract":"Three crude polysaccharides of RCP-A, B, and C were derived from the rhizome of Rohdea chinensis by means of hot water extraction, gradient ethanol precipitation and dialysis.Three different polysaccharides of RCP-C1-1, RCP-C1-2, and RCP-C1-3 were isolated using cellulose DEAE-52 and Sephadex G-200 chromatography from RCP-C.The average molecular weights of RCP-C1-1, RCP-C1-2, and RCP-C1-3 were measured as 1.5110 4 , 1.0610 4 , and 4.8610 3 by means of MALDI-TOF MS and UHPGC, respectively.All three polysaccharides were found to consist of D-fructose and D-glucose following hydrolysis and comparison with literature data.Based on FT-IR and NMR analysis, the polysaccharides were identified as inulin-type fructans, with their backbone composed of -D-glucopyranosyl-(12)-(-D-fructofuranosyl)n-(12)--Dfructofuranoside (nRCP-C1-1=79, nRCP-C1-2=58, nRCP-C1-3=27).The anti-tumor activity of the polysaccharides (RCP-A, B, and C) was evaluated in H22 tumor-bearing mice.The results suggested that the polysaccharides (RCP-A, RCP-B, and RCP-C) inhibited the growth of H22 hepatocellular.Further, the treated groups of RCP-A, RCP-B, and RCP-C exhibited improvements in body weight as well as spleen/thymus indexes in H22 tumor-bearing mice.","PeriodicalId":21053,"journal":{"name":"Records of Natural Products","volume":"1 3","pages":"339-346"},"PeriodicalIF":0.0,"publicationDate":"2024-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://doi.org/10.25135/rnp.454.2403.3165","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147901726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-03DOI: 10.25135/rnp.430.2320.2935
Nen-ling Zhang, Mengjia Guo, Bo Zhou, Xinman Zhao, Manqin Fu, Xiangchun Shen, Ling Tao
{"title":"Chemical Components and Their α-Glucosidase Inhibitory Activity from the Leaves of Ficus carica Linn.","authors":"Nen-ling Zhang, Mengjia Guo, Bo Zhou, Xinman Zhao, Manqin Fu, Xiangchun Shen, Ling Tao","doi":"10.25135/rnp.430.2320.2935","DOIUrl":"https://doi.org/10.25135/rnp.430.2320.2935","url":null,"abstract":"","PeriodicalId":21053,"journal":{"name":"Records of Natural Products","volume":"5 9","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139388692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-03DOI: 10.25135/rnp.433.2310.1929
Kee K. Kim, Da-min Jung, Sangsoo Lee, Jin P. Kim
{"title":"Allium hookeri Enhances Muscular Endurance of Mice by Increasing Muscle Cross-Sectional Area","authors":"Kee K. Kim, Da-min Jung, Sangsoo Lee, Jin P. Kim","doi":"10.25135/rnp.433.2310.1929","DOIUrl":"https://doi.org/10.25135/rnp.433.2310.1929","url":null,"abstract":"","PeriodicalId":21053,"journal":{"name":"Records of Natural Products","volume":"45 7","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139389066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-01DOI: 10.25135/rnp.428.2308.2861
J. Matejić, Anđela Dragićević, Miloš Jovanović, Lazar D. Žarković, Ana M. Džamić, Snežana Hinić, Dragana S. Pavlović
{"title":"Plant Products for Musculoskeletal, Respiratory, Circulatory, and Genitourinary Disorders in Eastern and South-Eastern Serbia – Folk Uses Comparison with Official Recommendations","authors":"J. Matejić, Anđela Dragićević, Miloš Jovanović, Lazar D. Žarković, Ana M. Džamić, Snežana Hinić, Dragana S. Pavlović","doi":"10.25135/rnp.428.2308.2861","DOIUrl":"https://doi.org/10.25135/rnp.428.2308.2861","url":null,"abstract":"","PeriodicalId":21053,"journal":{"name":"Records of Natural Products","volume":"37 15","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139128032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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