Pub Date : 2026-10-16DOI: 10.1021/acs.jafc.6c05419
Mei Li,Guangjian Li,Yutao Wang,Wei Xie,Xin Zhang,Canyu Zhang,Yufei Cao,Wen-Yong Lou
Abstract Acetate is a sustainable C2 feedstock derived from CO2 electroreduction, but its conversion to complex natural products is constrained by impaired cell growth and poor metabolic compatibility. Adaptive laboratory evolution generated an evolved Saccharomyces cerevisiae strain capable of producing 536.32 mg/L of p-coumaric acid from 15 g/L acetate as the sole carbon source. Genome- and transcriptome-level analyses subsequently revealed genetic factors associated with an enhanced acetate utilization. To evaluate acetate-supported flavonoid biosynthesis, a downstream flavonoid pathway was constructed in the parental chassis, yielding 19.55 mg/L of phloretin from acetate. Co-overexpression of EFG1 and ACS1, followed by fermentation optimization, increased the phloretin titer to 35.71 mg/L, reaching 78.71% of the glucose-based level. This work establishes S. cerevisiae as a viable platform for converting renewable C2 feedstocks into complex flavonoids and provides a practical strategy for the low-carbon bioproduction of high-value natural products.
{"title":"Metabolic Engineering\u0000of\u0000 Saccharomyces cerevisiae\u0000 for Flavonoid Synthesis from Acetate","authors":"Mei Li,Guangjian Li,Yutao Wang,Wei Xie,Xin Zhang,Canyu Zhang,Yufei Cao,Wen-Yong Lou","doi":"10.1021/acs.jafc.6c05419","DOIUrl":"https://doi.org/10.1021/acs.jafc.6c05419","url":null,"abstract":"Abstract Acetate is a sustainable C2 feedstock derived from CO2 electroreduction, but its conversion to complex natural products is constrained by impaired cell growth and poor metabolic compatibility. Adaptive laboratory evolution generated an evolved Saccharomyces cerevisiae strain capable of producing 536.32 mg/L of p-coumaric acid from 15 g/L acetate as the sole carbon source. Genome- and transcriptome-level analyses subsequently revealed genetic factors associated with an enhanced acetate utilization. To evaluate acetate-supported flavonoid biosynthesis, a downstream flavonoid pathway was constructed in the parental chassis, yielding 19.55 mg/L of phloretin from acetate. Co-overexpression of EFG1 and ACS1, followed by fermentation optimization, increased the phloretin titer to 35.71 mg/L, reaching 78.71% of the glucose-based level. This work establishes S. cerevisiae as a viable platform for converting renewable C2 feedstocks into complex flavonoids and provides a practical strategy for the low-carbon bioproduction of high-value natural products.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"68 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149209813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract Limited research on the sublethal effects and mechanisms of chiral pesticides, particularly ethiprole, on honeybee neurobehavior at the enantiomeric level has obscured our understanding of the survival risks facing honeybees. We used transcriptomic sequencing, behavioral assay, correlation analysis, and molecular modeling to investigate the enantioselective neurotoxic risk of ethiprole on honeybees and predict potential molecular mechanisms. Our findings indicate that S-ethiprole poses a significant neurotoxicity hazard by markedly disrupting multiple signaling pathways and altering the expression of more genes, primarily by targeting the Rho subfamily of the small GTPase superfamily. This disruption likely accounts for the impairment of the gustatory response behavior in honeybees. Furthermore, Rac protein emerges as a potential molecular target through which S-ethiprole influences signal transduction in honeybees, correlating with decreased Snodgrassella abundance and the accumulation of N-benzoyl-N′-(2-chlorophenyl)thiourea induced by S-ethiprole. These results contribute to understanding the enantioselective neurotoxic risks and mechanisms of chiral ethiprole on honeybees.
手性农药尤其是乙虫腈对蜜蜂对映体神经行为的亚致死效应及其机制的研究有限，这使得我们对蜜蜂面临的生存风险的理解变得模糊。我们使用转录组测序、行为分析、相关分析和分子模型来研究乙硫普罗对蜜蜂的对映选择性神经毒性风险，并预测潜在的分子机制。我们的研究结果表明，s -乙酯通过显著破坏多种信号通路和改变更多基因的表达，主要是通过靶向GTPase小超家族的Rho亚家族，具有显著的神经毒性危害。这种破坏可能是蜜蜂味觉反应行为受损的原因。此外，Rac蛋白作为S-ethiprole影响蜜蜂信号转导的潜在分子靶点，与S-ethiprole诱导的雪草丰度下降和n -苯甲酰- n ' -（2-氯苯基）硫脲积累有关。这些结果有助于了解手性乙虫腈对蜜蜂的对端选择性神经毒性风险和机制。
{"title":"Enantioselective\u0000Neurotoxic Risk of Ethiprole Exposure\u0000to Honeybees: Prediction of Multidimensional Mechanisms","authors":"Yonghong Zhang,Ziyu Chen,Yujia Wang,Keyi Ouyang,Xiaobo Wu","doi":"10.1021/acs.jafc.6c07615","DOIUrl":"https://doi.org/10.1021/acs.jafc.6c07615","url":null,"abstract":"Abstract Limited research on the sublethal effects and mechanisms of chiral pesticides, particularly ethiprole, on honeybee neurobehavior at the enantiomeric level has obscured our understanding of the survival risks facing honeybees. We used transcriptomic sequencing, behavioral assay, correlation analysis, and molecular modeling to investigate the enantioselective neurotoxic risk of ethiprole on honeybees and predict potential molecular mechanisms. Our findings indicate that S-ethiprole poses a significant neurotoxicity hazard by markedly disrupting multiple signaling pathways and altering the expression of more genes, primarily by targeting the Rho subfamily of the small GTPase superfamily. This disruption likely accounts for the impairment of the gustatory response behavior in honeybees. Furthermore, Rac protein emerges as a potential molecular target through which S-ethiprole influences signal transduction in honeybees, correlating with decreased Snodgrassella abundance and the accumulation of N-benzoyl-N′-(2-chlorophenyl)thiourea induced by S-ethiprole. These results contribute to understanding the enantioselective neurotoxic risks and mechanisms of chiral ethiprole on honeybees.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"73 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149210232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-10-16DOI: 10.1021/acs.jafc.6c11041
Hanyu Zhu,Longzheng Huang,Ximeng Liu,Yiyang Zhang,Wei-Bing Liu,Bang-Ce Ye
Abstract Vanillin is a high-value natural aromatic compound widely used in food, flavor, and pharmaceutical fields. Carboxylic acid reductase (CAR)-catalyzed vanillic acid bioreduction offers a green vanillin biosynthesis route, yet low catalytic activity and severe substrate inhibition limit its industrial application. Herein, we screened 10 bacterial CAR homologues and constructed superior chimeric CAR templates via domain recombination. Combined with structure-guided directed evolution and substrate tunnel remodeling, the optimized quadruple mutant M4 was obtained, exhibiting 4-fold higher catalytic efficiency compared with the wild-type MavCAR (and 1.6-fold higher than the parental chimera M0) and partially relieved activity decline at elevated substrate concentrations. Structural simulations clarified that tunnel expansion and hydrogen bond remodeling synergistically stabilized enzyme–substrate binding. The M4-based whole-cell system achieved 95% vanillin conversion (8.7 g·L–1) from 10 g·L–1 vanillic acid, and a two-step cascade system realized efficient de novo vanillin synthesis from glucose. This work provides a robust engineered CAR biocatalyst for green vanillin preparative-scale biosynthesis.
{"title":"Rational Engineering of Carboxylic Acid Reductase\u0000for Improved Catalytic Efficiency and Substrate Tolerance Enabling\u0000Green Vanillin Biosynthesis","authors":"Hanyu Zhu,Longzheng Huang,Ximeng Liu,Yiyang Zhang,Wei-Bing Liu,Bang-Ce Ye","doi":"10.1021/acs.jafc.6c11041","DOIUrl":"https://doi.org/10.1021/acs.jafc.6c11041","url":null,"abstract":"Abstract Vanillin is a high-value natural aromatic compound widely used in food, flavor, and pharmaceutical fields. Carboxylic acid reductase (CAR)-catalyzed vanillic acid bioreduction offers a green vanillin biosynthesis route, yet low catalytic activity and severe substrate inhibition limit its industrial application. Herein, we screened 10 bacterial CAR homologues and constructed superior chimeric CAR templates via domain recombination. Combined with structure-guided directed evolution and substrate tunnel remodeling, the optimized quadruple mutant M4 was obtained, exhibiting 4-fold higher catalytic efficiency compared with the wild-type MavCAR (and 1.6-fold higher than the parental chimera M0) and partially relieved activity decline at elevated substrate concentrations. Structural simulations clarified that tunnel expansion and hydrogen bond remodeling synergistically stabilized enzyme–substrate binding. The M4-based whole-cell system achieved 95% vanillin conversion (8.7 g·L–1) from 10 g·L–1 vanillic acid, and a two-step cascade system realized efficient de novo vanillin synthesis from glucose. This work provides a robust engineered CAR biocatalyst for green vanillin preparative-scale biosynthesis.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"13 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149210233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-10-16DOI: 10.1021/acs.jafc.6c10019
Qile Zhang,Zhengming Zhou,Hoi Wa Yeung,Yiu Ming Cheung,Yuen Lam Wong,Chufeng He,Shouhe Huang,Lu Jiang,Xiao Yu Tian,Zhen-Yu Chen,Wing Tak Wong
Abstract Aging-induced endothelial dysfunction is a core driver of age-related cardiovascular diseases (CVDs). In 20-month-old naturally aged mice, 12-week soybean polysaccharide (SP) supplementation restored endothelium-dependent relaxation, reduced aortic reactive oxygen species and VCAM-1 in the aortic endothelium, and attenuated colon senescence, improved gut barrier integrity, and reduced systemic inflammation, as shown by decreased serum LPS, IL-1β, TNF-α, and fecal and serum TMA/TMAO levels. SP enriched butyrate-producing gut bacteria, including Lachnospiraceae_NK4A136_group, and increased gut and serum butyrate, consistent with enhanced microbial short-chain fatty acid biosynthesis capacity predicted by PICRUSt2. Direct butyrate supplementation reproduced these vascular and colonic benefits. It activated PPARγ signaling and downregulated p21, ICAM-1, VCAM-1, and p16, whereas PPARα and PPARδ remained unchanged. These findings support a gut microbiota–butyrate–PPARγ/p21 axis through which SP protects against aging-related endothelial dysfunction, providing a nutritional basis for targeting the gut–vascular axis in cardiovascular aging.
{"title":"PPARγ/P21\u0000Signaling Mediates Butyrate-Driven\u0000Protection against Aging-Related Endothelial Dysfunction: A Novel\u0000Effect of Soybean Polysaccharide via the Gut-Vascular Axis","authors":"Qile Zhang,Zhengming Zhou,Hoi Wa Yeung,Yiu Ming Cheung,Yuen Lam Wong,Chufeng He,Shouhe Huang,Lu Jiang,Xiao Yu Tian,Zhen-Yu Chen,Wing Tak Wong","doi":"10.1021/acs.jafc.6c10019","DOIUrl":"https://doi.org/10.1021/acs.jafc.6c10019","url":null,"abstract":"Abstract Aging-induced endothelial dysfunction is a core driver of age-related cardiovascular diseases (CVDs). In 20-month-old naturally aged mice, 12-week soybean polysaccharide (SP) supplementation restored endothelium-dependent relaxation, reduced aortic reactive oxygen species and VCAM-1 in the aortic endothelium, and attenuated colon senescence, improved gut barrier integrity, and reduced systemic inflammation, as shown by decreased serum LPS, IL-1β, TNF-α, and fecal and serum TMA/TMAO levels. SP enriched butyrate-producing gut bacteria, including Lachnospiraceae_NK4A136_group, and increased gut and serum butyrate, consistent with enhanced microbial short-chain fatty acid biosynthesis capacity predicted by PICRUSt2. Direct butyrate supplementation reproduced these vascular and colonic benefits. It activated PPARγ signaling and downregulated p21, ICAM-1, VCAM-1, and p16, whereas PPARα and PPARδ remained unchanged. These findings support a gut microbiota–butyrate–PPARγ/p21 axis through which SP protects against aging-related endothelial dysfunction, providing a nutritional basis for targeting the gut–vascular axis in cardiovascular aging.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"17 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149209858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-10-16DOI: 10.1021/acs.jafc.6c11421
Fei Xia,Mengsi Li,Qingmei Liu,Liqiang Zhong,Yubao Cui,Chih-Jung Chang,Feng Lin,Yixiang Liu,Guangming Liu
Abstract Scylla paramamosain and Dermatophagoides farinae are dominant allergen sources in Asia, yet the molecular basis of their cross-reactivity remains poorly defined. Among 385 HDM-positive sera, 13.5% exhibited IgE reactivity to S. paramamosain extract, demonstrating serological cosensitization between crustacean and mite in southeastern China. Four homologous allergen pairs, namely, tropomyosin (TM), arginine kinase (AK), troponin C (TnC), and triosephosphate isomerase (TIM), shared >50% sequence identity and were all cross-recognized by IgG on Western blot. Basophil activation testing nevertheless distinguished the pairs functionally, as TM and AK activated basophils in 95% and 88% of cross-reactive patients, whereas TnC and TIM did not reach statistical significance at the group level. AK, conventionally ranked below TM in crustacean allergy, therefore, contributes almost equally to cross-reactivity. Cross-inhibition ELISA further demonstrated directional asymmetry in IgE cross-reactivity, and eight and nine conserved B-cell epitope regions were mapped on TM and AK. These findings clarify the allergen basis of crab-mite cosensitization and support AK-inclusive component-resolved diagnosis.
{"title":"Cross-Reactivity\u0000between\u0000 Scylla paramamosain\u0000 and\u0000 Dermatophagoides farinae\u0000 : Identification\u0000of Tropomyosin and Arginine Kinase as Major Cross-Reactive Allergens","authors":"Fei Xia,Mengsi Li,Qingmei Liu,Liqiang Zhong,Yubao Cui,Chih-Jung Chang,Feng Lin,Yixiang Liu,Guangming Liu","doi":"10.1021/acs.jafc.6c11421","DOIUrl":"https://doi.org/10.1021/acs.jafc.6c11421","url":null,"abstract":"Abstract Scylla paramamosain and Dermatophagoides farinae are dominant allergen sources in Asia, yet the molecular basis of their cross-reactivity remains poorly defined. Among 385 HDM-positive sera, 13.5% exhibited IgE reactivity to S. paramamosain extract, demonstrating serological cosensitization between crustacean and mite in southeastern China. Four homologous allergen pairs, namely, tropomyosin (TM), arginine kinase (AK), troponin C (TnC), and triosephosphate isomerase (TIM), shared >50% sequence identity and were all cross-recognized by IgG on Western blot. Basophil activation testing nevertheless distinguished the pairs functionally, as TM and AK activated basophils in 95% and 88% of cross-reactive patients, whereas TnC and TIM did not reach statistical significance at the group level. AK, conventionally ranked below TM in crustacean allergy, therefore, contributes almost equally to cross-reactivity. Cross-inhibition ELISA further demonstrated directional asymmetry in IgE cross-reactivity, and eight and nine conserved B-cell epitope regions were mapped on TM and AK. These findings clarify the allergen basis of crab-mite cosensitization and support AK-inclusive component-resolved diagnosis.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"22 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149209857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-10-15DOI: 10.1021/acs.jafc.6c08095
Minghao Sun, Sai Fan, Wei Liu, Jiaying Liu, Jing Zhan, Zhixiong Shi
Organophosphate esters (OPEs) are used as flame retardants and plasticizers and may enter preprepared dishes through raw materials, processing, and migration from food contact materials (FCMs). In this study, 28 OPEs and 13 mOPEs were determined in 347 commercially available preprepared dish samples purchased in Beijing, China, and migration experiments were conducted using 42 independent FCM samples. OPEs were widely detected in preprepared dishes (median: 9.04 ng/g wet weight (ww)), while mOPEs had much lower levels (median of 1.40 ng/g ww). Compared with fresh animal-origin foods, the dishes showed significantly higher OPE concentrations. Independent migration experiments further showed that several major OPEs could be released from the tested FCMs, demonstrating release potential consistent with a possible packaging contribution. A scenario-based exposure assessment indicated that OPE intake via preprepared dish consumption was below available health-based guidance values, while uncertainties remain because consumption data specific to preprepared dishes and toxicological thresholds for many OPEs/mOPEs are lacking.
{"title":"Organophosphate Esters and Their Metabolites in Commercially Available Preprepared Dishes: Occurrence, Migration from Food Contact Materials, and Dietary Exposure.","authors":"Minghao Sun, Sai Fan, Wei Liu, Jiaying Liu, Jing Zhan, Zhixiong Shi","doi":"10.1021/acs.jafc.6c08095","DOIUrl":"https://doi.org/10.1021/acs.jafc.6c08095","url":null,"abstract":"<p><p>Organophosphate esters (OPEs) are used as flame retardants and plasticizers and may enter preprepared dishes through raw materials, processing, and migration from food contact materials (FCMs). In this study, 28 OPEs and 13 mOPEs were determined in 347 commercially available preprepared dish samples purchased in Beijing, China, and migration experiments were conducted using 42 independent FCM samples. OPEs were widely detected in preprepared dishes (median: 9.04 ng/g wet weight (ww)), while mOPEs had much lower levels (median of 1.40 ng/g ww). Compared with fresh animal-origin foods, the dishes showed significantly higher OPE concentrations. Independent migration experiments further showed that several major OPEs could be released from the tested FCMs, demonstrating release potential consistent with a possible packaging contribution. A scenario-based exposure assessment indicated that OPE intake via preprepared dish consumption was below available health-based guidance values, while uncertainties remain because consumption data specific to preprepared dishes and toxicological thresholds for many OPEs/mOPEs are lacking.</p>","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":" ","pages":""},"PeriodicalIF":6.7,"publicationDate":"2026-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149206392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract Shrimp shell waste represents an abundant marine biomass containing chitin, proteins, minerals, and carotenoids that can be valorized through integrated biorefinery processing. Shrimp shell waste was bioprocessed to obtain chitin and bioactive coproducts using colactic acid fermentation (Co-LAF) and alkaline protease post-treatment. Mature coconut water was used as the sole nutrient source during Co-LAF, achieving demineralization (DM) efficiency >96% and deproteination (DP) efficiency >60%. Enzymatic post-treatment further increased DM to >99% and DP to >98%. Scale-up in a stirred-tank bioreactor produced comparable DM and DP efficiencies with a chitin yield of 25.1 ± 0.04%. The extracted chitin exhibited higher crystallinity (80.3%) and degree of acetylation (∼100%) than chemically extracted chitin. Liquid fractions containing calcium, protein hydrolysates, and carotenoids were characterized for antioxidant-related properties and GC–MS metabolites, indicating their potential for food, feed, and biobased material applications.
{"title":"Bioprocessing of\u0000Shrimp Shell Waste into Chitin and\u0000Functional Ingredients with High Bioactive Activity","authors":"Jariya Ruangwicha,Benjamas Cheirsilp,Wasana Suyotha","doi":"10.1021/acs.jafc.6c09636","DOIUrl":"https://doi.org/10.1021/acs.jafc.6c09636","url":null,"abstract":"Abstract Shrimp shell waste represents an abundant marine biomass containing chitin, proteins, minerals, and carotenoids that can be valorized through integrated biorefinery processing. Shrimp shell waste was bioprocessed to obtain chitin and bioactive coproducts using colactic acid fermentation (Co-LAF) and alkaline protease post-treatment. Mature coconut water was used as the sole nutrient source during Co-LAF, achieving demineralization (DM) efficiency &gt;96% and deproteination (DP) efficiency &gt;60%. Enzymatic post-treatment further increased DM to &gt;99% and DP to &gt;98%. Scale-up in a stirred-tank bioreactor produced comparable DM and DP efficiencies with a chitin yield of 25.1 ± 0.04%. The extracted chitin exhibited higher crystallinity (80.3%) and degree of acetylation (∼100%) than chemically extracted chitin. Liquid fractions containing calcium, protein hydrolysates, and carotenoids were characterized for antioxidant-related properties and GC–MS metabolites, indicating their potential for food, feed, and biobased material applications.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"17 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149209864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract This study investigated the effects of casein peptides on constipation-predominant irritable bowel syndrome (IBS-C) in mice. Results revealed that oral administration of casein hydrolyzate significantly attenuated constipation symptoms, reduced visceral hypersensitivity, improved intestinal barrier integrity, suppressed inflammatory responses, and restored serum neurotransmitter balance in IBS-C mice. Casein hydrolyzate did not alter guanylyl cyclase C (GC-C) mRNA levels but significantly increased cyclic guanosine-3‘,5′-monophosphate (cGMP) levels in the colon of IBS-C mice, suggesting the possible involvement of the GC-C/cGMP signaling pathway in the above effects. Additionally, nine casein peptides with potential GC-C agonistic activity were identified through an integrated in silico screening and ex vivo functional evaluation, among which PFPGPIPN showed the highest activity. Moreover, in vivo experiments confirmed that PFPGPIPN significantly ameliorated IBS-C symptoms and elevated colonic cGMP levels in mice. These findings highlight the potential of casein peptides as bioactive ingredients in functional foods for the management of IBS-C.
{"title":"Ameliorative Effects\u0000of Casein Peptides on Constipation-Predominant\u0000Irritable Bowel Syndrome: Involvement of the GC-C/cGMP Signaling Pathway","authors":"Zhiqing Lin,Yingjie Wang,Ying Su,Jiayi Li,Jian He,Sufang Duan,Wenyi Zhang,Xuebo Liu,Long Ding","doi":"10.1021/acs.jafc.6c00778","DOIUrl":"https://doi.org/10.1021/acs.jafc.6c00778","url":null,"abstract":"Abstract This study investigated the effects of casein peptides on constipation-predominant irritable bowel syndrome (IBS-C) in mice. Results revealed that oral administration of casein hydrolyzate significantly attenuated constipation symptoms, reduced visceral hypersensitivity, improved intestinal barrier integrity, suppressed inflammatory responses, and restored serum neurotransmitter balance in IBS-C mice. Casein hydrolyzate did not alter guanylyl cyclase C (GC-C) mRNA levels but significantly increased cyclic guanosine-3‘,5′-monophosphate (cGMP) levels in the colon of IBS-C mice, suggesting the possible involvement of the GC-C/cGMP signaling pathway in the above effects. Additionally, nine casein peptides with potential GC-C agonistic activity were identified through an integrated in silico screening and ex vivo functional evaluation, among which PFPGPIPN showed the highest activity. Moreover, in vivo experiments confirmed that PFPGPIPN significantly ameliorated IBS-C symptoms and elevated colonic cGMP levels in mice. These findings highlight the potential of casein peptides as bioactive ingredients in functional foods for the management of IBS-C.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"14 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149209865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract Paeoniae Radix Alba (PRA) polysaccharides are promising food-derived bioactives, yet their gut-mediated effects on rheumatoid arthritis (RA) remain unclear. Here, BP-1 was purified, structurally characterized, and evaluated in collagen-induced arthritis (CIA) rats and lipopolysaccharide (LPS)-injured Caco-2 cells. BP-1 was a 14.124 kDa neutral polysaccharide mainly composed of glucose and mannose. NMR analysis identified →4)-α-D-Glcp-(1→, →4,6)-α-D-Glcp-(1→, α-T-D-Glcp-(1→, →4,6)-β-D-Manp-(1→, and α-T-D-Galp-(1→ residues. BP-1 alleviated arthritis severity, joint pathology, systemic inflammation, and colonic injury, while improving intestinal barrier integrity in vivo and in vitro. These effects were potentially associated with suppression of TLR4/MyD88/NF-κB signaling, restoration of immune homeostasis, and modulation of gut microbiota and fecal metabolites. These findings support BP-1 as a potential functional food ingredient for inflammation-associated intestinal and immune disorders.
{"title":"A Paeoniae Radix\u0000Alba Polysaccharide Fraction BP-1\u0000Restores Intestinal Barrier Function and Gut Microbiota–Metabolic\u0000Homeostasis in Collagen-Induced Arthritis Rats","authors":"Yongchun Wang,Yongxi Wu,Yuhan Xie,Qiulu Zhao,Jianing Liu,Meihua Jiang,Yinlu Zhang,Songyang Xia,Wenshuo Zhang,Hao Yue,Zifeng Pi","doi":"10.1021/acs.jafc.6c06645","DOIUrl":"https://doi.org/10.1021/acs.jafc.6c06645","url":null,"abstract":"Abstract Paeoniae Radix Alba (PRA) polysaccharides are promising food-derived bioactives, yet their gut-mediated effects on rheumatoid arthritis (RA) remain unclear. Here, BP-1 was purified, structurally characterized, and evaluated in collagen-induced arthritis (CIA) rats and lipopolysaccharide (LPS)-injured Caco-2 cells. BP-1 was a 14.124 kDa neutral polysaccharide mainly composed of glucose and mannose. NMR analysis identified →4)-α-D-Glcp-(1→, →4,6)-α-D-Glcp-(1→, α-T-D-Glcp-(1→, →4,6)-β-D-Manp-(1→, and α-T-D-Galp-(1→ residues. BP-1 alleviated arthritis severity, joint pathology, systemic inflammation, and colonic injury, while improving intestinal barrier integrity in vivo and in vitro. These effects were potentially associated with suppression of TLR4/MyD88/NF-κB signaling, restoration of immune homeostasis, and modulation of gut microbiota and fecal metabolites. These findings support BP-1 as a potential functional food ingredient for inflammation-associated intestinal and immune disorders.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"20 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149209860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract Type III effector proteins (T3Es) are pivotal orchestrators of host–microbe interactions and essential for establishing symbiosis, thereby influencing soybean growth and productivity through modulation of symbiotic nitrogen fixation (SNF). While the central role of the T3E NopD in soybean SNF is recognized, its mechanistic underpinnings have remained elusive. In this study, we determined that GmPP2C, whose expression is regulated by NopD, serves as a regulator of nodulation. Overexpression of GmPP2C suppressed infection-thread formation and markedly reduced nodule number, whereas CRISPR/Cas9-mediated disruption enhanced infection events and partially rescued nodulation defects of NopD deficiency. Transcriptome profiling and complementation assays indicate GmPP2C inhibits nodulation by repressing ENOD93. Loss of GmPP2C increased per-plant yield without altering plant architecture or seed quality. These findings delineate the NopD–GmPP2C–ENOD93 regulatory module governing soybean nodulation and pinpoint GmPP2C as a compelling target for molecular breeding to enhance nitrogen-fixation efficiency and bolster crop yields.
III型效应蛋白（Type III effector protein, T3Es）是宿主与微生物相互作用的关键协调者，是建立共生关系的必要条件，从而通过调节共生固氮（symbiotic nitrogen fixation， SNF）影响大豆的生长和产量。虽然T3E NopD在大豆SNF中的核心作用已被认识到，但其机制基础仍然难以捉摸。在这项研究中，我们确定GmPP2C的表达受NopD的调节，作为结瘤的调节剂。过表达GmPP2C抑制了感染线的形成，显著减少了结节数量，而CRISPR/ cas9介导的破坏增强了感染事件，部分挽救了NopD缺乏症的结节缺陷。转录组分析和互补分析表明，GmPP2C通过抑制ENOD93来抑制结瘤。损失GmPP2C可提高单株产量，但不会改变植株结构或种子质量。这些发现描述了控制大豆结瘤的NopD-GmPP2C-ENOD93调控模块，并指出GmPP2C是提高固氮效率和提高作物产量的分子育种的一个引人注目的目标。
{"title":"The Rhizobial Type\u0000III Effector NopD Regulates the\u0000 GmPP2C-ENOD93\u0000 Axis\u0000to Control Symbiotic Nodulation and Yield\u0000in Soybean","authors":"Liang Mi,Liying Lu,Jiake Sun,Yansong Li,Chao Ma,Shengnan Ma,Mingliang Yang,Siyan Hong,Miao Wang,Lin Chen,Chunyan Liu,Dawei Xin,Qingshan Chen,Jinhui Wang","doi":"10.1021/acs.jafc.6c07326","DOIUrl":"https://doi.org/10.1021/acs.jafc.6c07326","url":null,"abstract":"Abstract Type III effector proteins (T3Es) are pivotal orchestrators of host–microbe interactions and essential for establishing symbiosis, thereby influencing soybean growth and productivity through modulation of symbiotic nitrogen fixation (SNF). While the central role of the T3E NopD in soybean SNF is recognized, its mechanistic underpinnings have remained elusive. In this study, we determined that GmPP2C, whose expression is regulated by NopD, serves as a regulator of nodulation. Overexpression of GmPP2C suppressed infection-thread formation and markedly reduced nodule number, whereas CRISPR/Cas9-mediated disruption enhanced infection events and partially rescued nodulation defects of NopD deficiency. Transcriptome profiling and complementation assays indicate GmPP2C inhibits nodulation by repressing ENOD93. Loss of GmPP2C increased per-plant yield without altering plant architecture or seed quality. These findings delineate the NopD–GmPP2C–ENOD93 regulatory module governing soybean nodulation and pinpoint GmPP2C as a compelling target for molecular breeding to enhance nitrogen-fixation efficiency and bolster crop yields.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"68 1 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149209861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：