Pub Date : 2021-06-30DOI: 10.30476/MEJC.2021.89770.1549
M. Latif, S. Tirmazy, Dalia M El-Shourbagy, A. Basit, Kaltar Das, M. Omara, Dina Hamza, Susheel Kumar, N. Salim, S. Sajan
Dear Editor-in-Chief, In March 2020, the World Health Organization (WHO) characterized corona virus disease-19 (COVID-19) as a pandemic. 1 Over 80 million people have been infected and 1.5 million confirmed deaths have been reported globally. It is not entirely clear whether cancer diagnosis and treatments increase the risk of complication in the COVID-19infection. Several studies have reported higher rates of intensive care unit admission, invasive ventilation, and deaths due to the COVID-19 infection in cancer patients. 2
{"title":"Provision of Oncology Services in Dubai Health Authority during First Wave of COVID-19 Pandemic: A Pragmatic Approach","authors":"M. Latif, S. Tirmazy, Dalia M El-Shourbagy, A. Basit, Kaltar Das, M. Omara, Dina Hamza, Susheel Kumar, N. Salim, S. Sajan","doi":"10.30476/MEJC.2021.89770.1549","DOIUrl":"https://doi.org/10.30476/MEJC.2021.89770.1549","url":null,"abstract":"Dear Editor-in-Chief, In March 2020, the World Health Organization (WHO) characterized corona virus disease-19 (COVID-19) as a pandemic. 1 Over 80 million people have been infected and 1.5 million confirmed deaths have been reported globally. It is not entirely clear whether cancer diagnosis and treatments increase the risk of complication in the COVID-19infection. Several studies have reported higher rates of intensive care unit admission, invasive ventilation, and deaths due to the COVID-19 infection in cancer patients. 2","PeriodicalId":44005,"journal":{"name":"Middle East Journal of Cancer","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2021-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46758060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-28DOI: 10.30476/MEJC.2021.86476.1346
E. Moustafa, E. Hassan, Mohamed Mahmoud, A. Mahmoud, Mohamed A. A. Ghaliony
Background: The increasing incidence of hepatocellular carcinoma (HCC) is a challenging health problem worldwide with poor prognosis and limited treatment options. The association between metabolic factors and HCC has been documented, however, there is a shortage of data about this association in our locality. Therefore, we aimed to determine the pattern of lipid profile in cirrhotic patients with HCC and investigate the association between dyslipidemia and HCC. Method: In this case-control hospital-based study, serum lipid profile [total cholesterol, triglycerides, low-density lipoprotein (LDL), high-density lipoprotein (HDL), and very-low-density lipoprotein (VLDL)] was determined in 100 patients with cirrhosis and 100 patients with cirrhosis and HCC. Multivariate analysis of HCC risk factors was done. Results: Metabolic syndrome, hypertriglyceridemia, hypercholesterolemia, high LDL, and combined dyslipidemia were significantly more frequent in HCC patients than non-HCC patients. Low HDL and dyslipidemia were significantly associated with the late HCC stages and LDL levels were significantly correlated with α-fetoprotein levels. There was a tendency towards increasing the values of the other lipid parameters in advanced stages. Metabolic syndrome and combined dyslipidemia were associated with HCC risk. Conclusion: Deranged lipid profiles were common in HCC patients. Metabolic syndrome and combined dyslipidemia could be potential risk factors for HCC and may offer a useful strategy for risk stratification; thus, their control can reduce the HCC burden.
{"title":"Deranged Lipid Profiles and Hepatocellular carcinoma: Clinical Significance and Association","authors":"E. Moustafa, E. Hassan, Mohamed Mahmoud, A. Mahmoud, Mohamed A. A. Ghaliony","doi":"10.30476/MEJC.2021.86476.1346","DOIUrl":"https://doi.org/10.30476/MEJC.2021.86476.1346","url":null,"abstract":"Background: The increasing incidence of hepatocellular carcinoma (HCC) is a challenging health problem worldwide with poor prognosis and limited treatment options. The association between metabolic factors and HCC has been documented, however, there is a shortage of data about this association in our locality. Therefore, we aimed to determine the pattern of lipid profile in cirrhotic patients with HCC and investigate the association between dyslipidemia and HCC. \u0000Method: In this case-control hospital-based study, serum lipid profile [total cholesterol, triglycerides, low-density lipoprotein (LDL), high-density lipoprotein (HDL), and very-low-density lipoprotein (VLDL)] was determined in 100 patients with cirrhosis and 100 patients with cirrhosis and HCC. Multivariate analysis of HCC risk factors was done. \u0000Results: Metabolic syndrome, hypertriglyceridemia, hypercholesterolemia, high LDL, and combined dyslipidemia were significantly more frequent in HCC patients than non-HCC patients. Low HDL and dyslipidemia were significantly associated with the late HCC stages and LDL levels were significantly correlated with α-fetoprotein levels. There was a tendency towards increasing the values of the other lipid parameters in advanced stages. Metabolic syndrome and combined dyslipidemia were associated with HCC risk. \u0000Conclusion: Deranged lipid profiles were common in HCC patients. Metabolic syndrome and combined dyslipidemia could be potential risk factors for HCC and may offer a useful strategy for risk stratification; thus, their control can reduce the HCC burden.","PeriodicalId":44005,"journal":{"name":"Middle East Journal of Cancer","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2021-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43435629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-26DOI: 10.30476/MEJC.2021.86467.1345
Soroosh Shahryarhesami, M. Heidari, Masoud Heidari, N. Sadighi
Background: Homeodomain transcriptional regulatory proteins, which are encoded by Homeobox (HOX) genes, play critical roles in both normal development and carcinogenesis. Previous studies have shown that the expression of HOX genes is deregulated in numerous tumors and this expression is specific to each cancer based on the arising embryonic origin tissue and the site of tumor. Method: In this in vitro study, the expression levels of HOXA10, CDX1, CDX2, TGIFLX, TGIFLY, and OCT1 genes were compared across 10 different human colorectal cancer cell lines with different differentiation stages. Subsequently, the effect of TGIFLX siRNA-mediated knockdown on the expression levels of CDX1, CDX2, and OCT1 genes was analyzed in SW948 cell line. Results: The obtained results revealed that these homeobox genes were differentially expressed in different colorectal cancer cell lines. Furthermore, the siRNA-mediated knockdown of TGIFLX led to higher levels of CDX1, CDX2, and OCT1 expression. Conclusion: Our data suggested that TGIFLX plays an important role in the upstream regulation of CDX1, CDX2, and OCT1 genes.
{"title":"Human Homeobox TGIFLX Regulates CDX1, CDX2, and OCT1 genes Expression in Colorectal Cancer Cell Lines","authors":"Soroosh Shahryarhesami, M. Heidari, Masoud Heidari, N. Sadighi","doi":"10.30476/MEJC.2021.86467.1345","DOIUrl":"https://doi.org/10.30476/MEJC.2021.86467.1345","url":null,"abstract":"Background: Homeodomain transcriptional regulatory proteins, which are encoded by Homeobox (HOX) genes, play critical roles in both normal development and carcinogenesis. Previous studies have shown that the expression of HOX genes is deregulated in numerous tumors and this expression is specific to each cancer based on the arising embryonic origin tissue and the site of tumor. \u0000Method: In this in vitro study, the expression levels of HOXA10, CDX1, CDX2, TGIFLX, TGIFLY, and OCT1 genes were compared across 10 different human colorectal cancer cell lines with different differentiation stages. Subsequently, the effect of TGIFLX siRNA-mediated knockdown on the expression levels of CDX1, CDX2, and OCT1 genes was analyzed in SW948 cell line. \u0000Results: The obtained results revealed that these homeobox genes were differentially expressed in different colorectal cancer cell lines. Furthermore, the siRNA-mediated knockdown of TGIFLX led to higher levels of CDX1, CDX2, and OCT1 expression. \u0000Conclusion: Our data suggested that TGIFLX plays an important role in the upstream regulation of CDX1, CDX2, and OCT1 genes.","PeriodicalId":44005,"journal":{"name":"Middle East Journal of Cancer","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2021-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48266526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-26DOI: 10.30476/MEJC.2021.86529.1348
N. Namdari, M. Anbardar, M. Dehghani
Primary mediastinal germ cell tumors accounts for about 3-15% of the mediastinal malignancies. Nonseminomatous tumors make a small percentage of germ cell tumors. Treatment of mediastinal choriocarcinoma includes initial systemic chemotherapy, followed by complete resection of all residual tumors. However, patients with non-seminomatous tumors have very poor prognosis.
{"title":"Primary Mediastinal Choriocarcinoma in a Female Patient with Vaginal Bleeding: A Case Report","authors":"N. Namdari, M. Anbardar, M. Dehghani","doi":"10.30476/MEJC.2021.86529.1348","DOIUrl":"https://doi.org/10.30476/MEJC.2021.86529.1348","url":null,"abstract":"Primary mediastinal germ cell tumors accounts for about 3-15% of the mediastinal malignancies. Nonseminomatous tumors make a small percentage of germ cell tumors. Treatment of mediastinal choriocarcinoma includes initial systemic chemotherapy, followed by complete resection of all residual tumors. However, patients with non-seminomatous tumors have very poor prognosis.","PeriodicalId":44005,"journal":{"name":"Middle East Journal of Cancer","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2021-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44774740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-22DOI: 10.30476/MEJC.2021.86492.1347
Somayeh Mahmoodi Khatonabadi, S. Salami, R. Mirfakhraie, Roya Atabakhshian, M. Sirati-Sabet, B. Yaghmaei, Shiva Ghafghazi, S. Ziai
Background: Breast cancer is known to be one of the most prevalent malignancies in women worldwide. Umbelliprenin is a naturally-occurring component derived from plant species, which has shown anti-cancer properties. The present study aimed to evaluate the effect of umbelliprenin on the PI3K / Akt / ERK signaling pathway and their products HIF-1α / VEGF in the MDA-MB-231 cell line. Method: In this experimental study, the cytotoxic effect of umbelliprenin on MDA-MB-231 cells was evaluated using the MTT assay and the umbelliprenin concentrations of IC5 and IC10 were selected for the signaling pathway study. MDA-MB-231 cells were stimulated with EGF and CoCl2 and umbelliprenin IC5 and IC10 effects on gene expression and translation was studied. PI3K / Akt / mTOR / S6K / Erk1 and 2 / 4E-BP1 / HIF-1α / HIF-1β / EGFR / VEGFR and VEGF mRNA expression, and VEGF / HIF-1α proteins were evaluated employing real time PCR and western blot analysis, respectively. Results: The concentrations of umbelliprenin in IC10 and IC5 were 20 and 10 μM, respectively. Umbelliprenin, specifically IC10, significantly inhibited PI3K, ERK1, ERK2, Akt, mTOR, HIF1-, HIF1- mRNA, as well as HIF-1 and VEGF protein expression. Conclusion: Our results suggested that UMB, a cytotoxic agent, inhibits PI3K / Akt / ERK signal pathway in the CoCl2 or EGF-stimulated MDA-MB-231 cells.
{"title":"Umbelliprenin Inhibited Angiogenesis and Metastasis of MDA-MB-231 Cell Line through Downregulation of CoCl2 / EGF-Mediated PI3K / AKT / ERK Signaling","authors":"Somayeh Mahmoodi Khatonabadi, S. Salami, R. Mirfakhraie, Roya Atabakhshian, M. Sirati-Sabet, B. Yaghmaei, Shiva Ghafghazi, S. Ziai","doi":"10.30476/MEJC.2021.86492.1347","DOIUrl":"https://doi.org/10.30476/MEJC.2021.86492.1347","url":null,"abstract":"Background: Breast cancer is known to be one of the most prevalent malignancies in women worldwide. Umbelliprenin is a naturally-occurring component derived from plant species, which has shown anti-cancer properties. The present study aimed to evaluate the effect of umbelliprenin on the PI3K / Akt / ERK signaling pathway and their products HIF-1α / VEGF in the MDA-MB-231 cell line. \u0000Method: In this experimental study, the cytotoxic effect of umbelliprenin on MDA-MB-231 cells was evaluated using the MTT assay and the umbelliprenin concentrations of IC5 and IC10 were selected for the signaling pathway study. MDA-MB-231 cells were stimulated with EGF and CoCl2 and umbelliprenin IC5 and IC10 effects on gene expression and translation was studied. PI3K / Akt / mTOR / S6K / Erk1 and 2 / 4E-BP1 / HIF-1α / HIF-1β / EGFR / VEGFR and VEGF mRNA expression, and VEGF / HIF-1α proteins were evaluated employing real time PCR and western blot analysis, respectively. \u0000Results: The concentrations of umbelliprenin in IC10 and IC5 were 20 and 10 μM, respectively. Umbelliprenin, specifically IC10, significantly inhibited PI3K, ERK1, ERK2, Akt, mTOR, HIF1-, HIF1- mRNA, as well as HIF-1 and VEGF protein expression. \u0000Conclusion: Our results suggested that UMB, a cytotoxic agent, inhibits PI3K / Akt / ERK signal pathway in the CoCl2 or EGF-stimulated MDA-MB-231 cells.","PeriodicalId":44005,"journal":{"name":"Middle East Journal of Cancer","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2021-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48333975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-22DOI: 10.30476/MEJC.2021.86421.1344
A. Krishna, D. Fernandes, M. Athiyamaan, S. Shankar, Sandesh Rao, A. Hasib
Background: Testes being in close proximity to radiation fields in patients of rectal cancer treated with radiotherapy, inadvertently receive a part of the radiation dose. This study was conducted to evaluate the variation of male sex hormones during the course of radiotherapy in patients with rectal cancer. Method: In this single-institution prospective study, 20 patients with carcinoma rectum were included. The patients were treated with 3-dimensional conformal radiation therapy or intensity modulated radiation therapy technique to a dose of 50.4 Gy for five weeks. Serum testosterone, follicle stimulating hormone (FSH), and luteinizing hormone (LH) levels were obtained prior to and in the meantime of radiotherapy. The post treatment hormone levels were compared to the baseline values. The mean, percentage, standard deviation, and Paired t-test were used for statistical analysis. Results: The mean dose received by the testes was 2.65 Gy (1.96 Gy to 4.96 Gy), which accounted for 5.25% of the total dose. The baseline values of serum testosterone, FSH, and LH were 4.65±0.7 ng/ml, 7.57±1.2 mIU/ml, and 7.93±1.1 mIU/ml, respectively. There was a 32.1% drop in the post treatment testosterone levels compared to the baseline. There was a 77% rise in the post treatment FSH and 40.2% rise in the post treatment LH levels compared to the baseline. There was a significant difference in the rise of LH levels in the patients who received a testicular dose more than 2Gy compared to those who received a dose less than 2 Gy. Conclusion: Radiation therapy was found to have a significant acute impact on male sex hormones in patients receiving radiotherapy for rectal cancer.
{"title":"Assessment of Variations in Serum Testosterone, Follicle Stimulating Hormone, and Luteinizing Hormone Levels in Patients Receiving Radiotherapy for Rectal Cancer","authors":"A. Krishna, D. Fernandes, M. Athiyamaan, S. Shankar, Sandesh Rao, A. Hasib","doi":"10.30476/MEJC.2021.86421.1344","DOIUrl":"https://doi.org/10.30476/MEJC.2021.86421.1344","url":null,"abstract":"Background: Testes being in close proximity to radiation fields in patients of rectal cancer treated with radiotherapy, inadvertently receive a part of the radiation dose. This study was conducted to evaluate the variation of male sex hormones during the course of radiotherapy in patients with rectal cancer. \u0000Method: In this single-institution prospective study, 20 patients with carcinoma rectum were included. The patients were treated with 3-dimensional conformal radiation therapy or intensity modulated radiation therapy technique to a dose of 50.4 Gy for five weeks. Serum testosterone, follicle stimulating hormone (FSH), and luteinizing hormone (LH) levels were obtained prior to and in the meantime of radiotherapy. The post treatment hormone levels were compared to the baseline values. The mean, percentage, standard deviation, and Paired t-test were used for statistical analysis. \u0000Results: The mean dose received by the testes was 2.65 Gy (1.96 Gy to 4.96 Gy), which accounted for 5.25% of the total dose. The baseline values of serum testosterone, FSH, and LH were 4.65±0.7 ng/ml, 7.57±1.2 mIU/ml, and 7.93±1.1 mIU/ml, respectively. There was a 32.1% drop in the post treatment testosterone levels compared to the baseline. There was a 77% rise in the post treatment FSH and 40.2% rise in the post treatment LH levels compared to the baseline. There was a significant difference in the rise of LH levels in the patients who received a testicular dose more than 2Gy compared to those who received a dose less than 2 Gy. \u0000Conclusion: Radiation therapy was found to have a significant acute impact on male sex hormones in patients receiving radiotherapy for rectal cancer.","PeriodicalId":44005,"journal":{"name":"Middle East Journal of Cancer","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2021-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44545807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-22DOI: 10.30476/MEJC.2021.84775.1232
F. Jowkar, F. Aslani, Leila Samipour, Fatemeh Mohebpour, M. Hadibarhaghtalab, Negin Fazelzadeh Haghighi, K. Taghipour
Background: Basal cell carcinoma (BCC) is the most prevalent type of skin cancer in Iran. The determination of subtype of BCC plays an essential role in the diagnosis, recurrence rate, and outcome of patients. The present study was conducted to investigate the relationship between histopathologic subtypes and demographic data, history of radiation exposure, and past medical history in the Iranian population. Method: This retrospective cross-sectional study evaluated the patients with BCC referred to Faghihi hospital Shiraz Iran from 2012 to 2017. We examined all the patients with definite histologically diagnosed scalp BCC. The prevalence of different subtypes and its association with other variables were compared between the patients with and without chronic radio-dermatitis. A P-value of less than 0.05 was considered to be significant. Results: A total number of 161 patients with a cumulative of 439 BCC lesions participated in the study. The mean age of the patients was 64.2 (± 12.38) years old. Among the patients, 113 (70.2%) were men and 48 (29.8%) were women. The total prevalence of macro-nodular, micro-nodular, and mixed aggressive was 70.2%, 49.1%, and 41.6%, respectively. Multivariate logistic regression analysis showed that excessive sun exposure increased the chance of developing micronodular and mixed aggressive lesions by 3.21 (P=0.006) and 4.88 (P<0.001) times, respectively. Conclusion: BCC was more aggressive in chronic radio-dermatitis patients than that in non-radio-dermatitis patients. Moreover, it was significantly different regarding age, gender, appearance, and job distribution compared to non-radio-dermatitis patients. Thus, we could suggest that BCC in chronic radio-dermatitis should be regarded as a high-risk disease unless proven otherwise.
{"title":"Determination of the Frequency of Scalp Basal Cell Carcinoma Subtypes Based on Histopathology and Other Related Variables and its Comparison between Patients with and without Chronic Radio-Dermatitis","authors":"F. Jowkar, F. Aslani, Leila Samipour, Fatemeh Mohebpour, M. Hadibarhaghtalab, Negin Fazelzadeh Haghighi, K. Taghipour","doi":"10.30476/MEJC.2021.84775.1232","DOIUrl":"https://doi.org/10.30476/MEJC.2021.84775.1232","url":null,"abstract":"Background: Basal cell carcinoma (BCC) is the most prevalent type of skin cancer in Iran. The determination of subtype of BCC plays an essential role in the diagnosis, recurrence rate, and outcome of patients. The present study was conducted to investigate the relationship between histopathologic subtypes and demographic data, history of radiation exposure, and past medical history in the Iranian population. \u0000Method: This retrospective cross-sectional study evaluated the patients with BCC referred to Faghihi hospital Shiraz Iran from 2012 to 2017. We examined all the patients with definite histologically diagnosed scalp BCC. The prevalence of different subtypes and its association with other variables were compared between the patients with and without chronic radio-dermatitis. A P-value of less than 0.05 was considered to be significant. \u0000Results: A total number of 161 patients with a cumulative of 439 BCC lesions participated in the study. The mean age of the patients was 64.2 (± 12.38) years old. Among the patients, 113 (70.2%) were men and 48 (29.8%) were women. The total prevalence of macro-nodular, micro-nodular, and mixed aggressive was 70.2%, 49.1%, and 41.6%, respectively. Multivariate logistic regression analysis showed that excessive sun exposure increased the chance of developing micronodular and mixed aggressive lesions by 3.21 (P=0.006) and 4.88 (P<0.001) times, respectively. \u0000Conclusion: BCC was more aggressive in chronic radio-dermatitis patients than that in non-radio-dermatitis patients. Moreover, it was significantly different regarding age, gender, appearance, and job distribution compared to non-radio-dermatitis patients. Thus, we could suggest that BCC in chronic radio-dermatitis should be regarded as a high-risk disease unless proven otherwise.","PeriodicalId":44005,"journal":{"name":"Middle East Journal of Cancer","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2021-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45790253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-20DOI: 10.30476/MEJC.2021.86545.1352
Bahareh Yazdani, Z. Mousavi, Mohsen Ehsan, Behjat Kalantary Khandany, G. Hassanshahi
Background: The CXCR4 receptor along with CXCL12 is believed to have an effect on the onset, progression, migration, and treatment complications and improve AML (acute myeloid leukemia) treatment outcomes. In this study, we investigated the impact of (7+3) chemotherapy protocol on the expression of CXCR4 and its related ligand CXCL12. Method: In this case-control study, specimens were collected before and after the first cycle of chemotherapy of AML-M4 and AML-M5 patients. RT-PCR and flow cytometry techniques tested the CXCR4 expression. ELISA was used for measuring the serum level of CXCL12. Two samples, t-Test and Paired t-Test, were utilized for data analysis. Results: We found that CXCR4 expression by Lymphocyte cells after chemotherapy was approximately similar to the CXCR4 expression in the healthy subjects. Moreover, CXCR4 expression was high prior to chemotherapy. The serum level of CXCL12 considerably increased in the patients before chemotherapy. However, after chemotherapy, CXCL12 was found to reach the baseline level in comparison to the healthy control group. Conclusion: The (7+3) current chemotherapy inhibited CXCL12. Therefore, controlling chemokines along with chemotherapy in AML patients might be conducive to the treatment process or even prevent the relapse of the disease.
{"title":"Does the Chemotherapy Protocol Affect CXCL12/CXCR4 Axes in Acute Myeloid Leukemia Patients with Monocytic Differentiation?","authors":"Bahareh Yazdani, Z. Mousavi, Mohsen Ehsan, Behjat Kalantary Khandany, G. Hassanshahi","doi":"10.30476/MEJC.2021.86545.1352","DOIUrl":"https://doi.org/10.30476/MEJC.2021.86545.1352","url":null,"abstract":"Background: The CXCR4 receptor along with CXCL12 is believed to have an effect on the onset, progression, migration, and treatment complications and improve AML (acute myeloid leukemia) treatment outcomes. In this study, we investigated the impact of (7+3) chemotherapy protocol on the expression of CXCR4 and its related ligand CXCL12. Method: In this case-control study, specimens were collected before and after the first cycle of chemotherapy of AML-M4 and AML-M5 patients. RT-PCR and flow cytometry techniques tested the CXCR4 expression. ELISA was used for measuring the serum level of CXCL12. Two samples, t-Test and Paired t-Test, were utilized for data analysis. Results: We found that CXCR4 expression by Lymphocyte cells after chemotherapy was approximately similar to the CXCR4 expression in the healthy subjects. Moreover, CXCR4 expression was high prior to chemotherapy. The serum level of CXCL12 considerably increased in the patients before chemotherapy. However, after chemotherapy, CXCL12 was found to reach the baseline level in comparison to the healthy control group. Conclusion: The (7+3) current chemotherapy inhibited CXCL12. Therefore, controlling chemokines along with chemotherapy in AML patients might be conducive to the treatment process or even prevent the relapse of the disease.","PeriodicalId":44005,"journal":{"name":"Middle East Journal of Cancer","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2021-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45337172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-20DOI: 10.30476/MEJC.2021.87469.1421
M. Dabbaghmanesh, Bahar Rezaei, M. Haghshenas, N. Montazeri-Najafabady, Rajeeh Mohammadian Amiri, N. Erfani
Background: The aim of this study was to investigate the association between thyroid cancer and 16C/A single nucleotide polymorphism (SNP) in C-C motif chemokine 22 (CCL22) as well as 1014C/T SNP in C-C chemokine receptor type 4 (CCR4). Method: In this case-control study, Polymerase Chain Reaction Restriction-Fragment Length Polymorphism (PCR-RFLP) was performed for 113 thyroid cancer patients and 112 age-sex matched healthy controls to investigate the genotype distribution. Results: At position 16C/A in CCL22, 95 patients (84.3%) were found to have CC genotype while 17 individuals (14.8%) inherited CA genotype and 1 (0.9%) had AA genotype. In the control group, 92 volunteers (82.1%) inherited CC genotype, 18 individuals (16.1%) had CA genotype, and 2 (1.8%) had AA genotype. The frequency of CC, CT, and TT genotypes of 1014C/T SNP in CCR4 gene was 60 (53.1%), 43 (38.1%), and 10 (8.8%) in the patients, and 57 (53.3%), 43 (40.2%), and 7 (6.5%) in the control group, respectively. There were no statistically significant differences between the patients and controls in terms of 16C/A polymorphism in CCL22 (P= 0.816) and 1014C/T SNP in CCR4 1014C/T gene position (P=0.801). Nevertheless, the study of their association indicated that inheriting the CC genotype of CCR4 is significantly associated with higher stages (stages 3 and 4) in thyroid cancer. Conclusion: 1014C/T genetic variation in CCR4 and 16C/A polymorphism in CCL22 were not found to have a role in the incidence of thyroid cancer. Inheriting CC genotype at 1014 locus in CCR4 may, however, affect cancer progression in patients with thyroid cancer.
{"title":"CCR4 1014C/T and CCL22 16C/A Genetic Variations in the Iranian Patients with Thyroid Cancer","authors":"M. Dabbaghmanesh, Bahar Rezaei, M. Haghshenas, N. Montazeri-Najafabady, Rajeeh Mohammadian Amiri, N. Erfani","doi":"10.30476/MEJC.2021.87469.1421","DOIUrl":"https://doi.org/10.30476/MEJC.2021.87469.1421","url":null,"abstract":"Background: The aim of this study was to investigate the association between thyroid cancer and 16C/A single nucleotide polymorphism (SNP) in C-C motif chemokine 22 (CCL22) as well as 1014C/T SNP in C-C chemokine receptor type 4 (CCR4). \u0000Method: In this case-control study, Polymerase Chain Reaction Restriction-Fragment Length Polymorphism (PCR-RFLP) was performed for 113 thyroid cancer patients and 112 age-sex matched healthy controls to investigate the genotype distribution. \u0000Results: At position 16C/A in CCL22, 95 patients (84.3%) were found to have CC genotype while 17 individuals (14.8%) inherited CA genotype and 1 (0.9%) had AA genotype. In the control group, 92 volunteers (82.1%) inherited CC genotype, 18 individuals (16.1%) had CA genotype, and 2 (1.8%) had AA genotype. The frequency of CC, CT, and TT genotypes of 1014C/T SNP in CCR4 gene was 60 (53.1%), 43 (38.1%), and 10 (8.8%) in the patients, and 57 (53.3%), 43 (40.2%), and 7 (6.5%) in the control group, respectively. There were no statistically significant differences between the patients and controls in terms of 16C/A polymorphism in CCL22 (P= 0.816) and 1014C/T SNP in CCR4 1014C/T gene position (P=0.801). Nevertheless, the study of their association indicated that inheriting the CC genotype of CCR4 is significantly associated with higher stages (stages 3 and 4) in thyroid cancer. \u0000Conclusion: 1014C/T genetic variation in CCR4 and 16C/A polymorphism in CCL22 were not found to have a role in the incidence of thyroid cancer. Inheriting CC genotype at 1014 locus in CCR4 may, however, affect cancer progression in patients with thyroid cancer.","PeriodicalId":44005,"journal":{"name":"Middle East Journal of Cancer","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2021-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49668174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-12DOI: 10.30476/MEJC.2021.85875.1312
Y. D. Cakil, Zeynep Akbulut, Hatice Maras, D. Kayalı, R. Aktas
Backgroundː The importance of extracellular matrix (ECM) components in the progression of hepatocellular carcinoma (HCC) has been shown in many studies. Although restoring or activating apoptosis in tumors is an active area of cancer research, little is known regarding the effects of collagen type I, the main ECM component in the liver, on apoptosis of HCC cells. Here, we investigated the apoptotic profiles of HCC cells in a microenvironment with collagen type I. Methodː In this in vitro study, we assessed the effects of collagen type I on HepG2 cells in pre-confluent and confluent states. We determined the mRNA levels of 25 genes, which are the key players of apoptosis. Flow cytometry-based apoptosis detection was performed by use ofAnnexin V/PI staining. Confocal laser scanning microscopy was used to assess P53 immunofluorescence in the cells. Resultsː The microenvironment with collagen type I and the confluency state of HepG2 cells affected the expression of 13 genes involved in apoptosis. We observed no significant change in the number of cells undergoing apoptosis depending on the confluency state or the presence of collagen type I. P53 immunofluorescence demonstrated no significant changes. Conclusionː We propose an apoptotic balance concerning overall cell survival which might be caused by the counteraction of positive and negative mediators of apoptosis. This study might provide data for the involvement of collagen type I in apoptotic responses of HCC, and contribute to a better understanding of cancer microenvironment.
{"title":"Collagen Type IInduces a Balance in the Expression of Anti- and Pro-Apoptotic Genes in Hepatocellular Carcinoma Cells","authors":"Y. D. Cakil, Zeynep Akbulut, Hatice Maras, D. Kayalı, R. Aktas","doi":"10.30476/MEJC.2021.85875.1312","DOIUrl":"https://doi.org/10.30476/MEJC.2021.85875.1312","url":null,"abstract":"Backgroundː The importance of extracellular matrix (ECM) components in the progression of hepatocellular carcinoma (HCC) has been shown in many studies. Although restoring or activating apoptosis in tumors is an active area of cancer research, little is known regarding the effects of collagen type I, the main ECM component in the liver, on apoptosis of HCC cells. Here, we investigated the apoptotic profiles of HCC cells in a microenvironment with collagen type I. \u0000Methodː In this in vitro study, we assessed the effects of collagen type I on HepG2 cells in pre-confluent and confluent states. We determined the mRNA levels of 25 genes, which are the key players of apoptosis. Flow cytometry-based apoptosis detection was performed by use ofAnnexin V/PI staining. Confocal laser scanning microscopy was used to assess P53 immunofluorescence in the cells. \u0000Resultsː The microenvironment with collagen type I and the confluency state of HepG2 cells affected the expression of 13 genes involved in apoptosis. We observed no significant change in the number of cells undergoing apoptosis depending on the confluency state or the presence of collagen type I. P53 immunofluorescence demonstrated no significant changes. \u0000Conclusionː We propose an apoptotic balance concerning overall cell survival which might be caused by the counteraction of positive and negative mediators of apoptosis. This study might provide data for the involvement of collagen type I in apoptotic responses of HCC, and contribute to a better understanding of cancer microenvironment.","PeriodicalId":44005,"journal":{"name":"Middle East Journal of Cancer","volume":"1 1","pages":""},"PeriodicalIF":0.4,"publicationDate":"2021-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70076711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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